WO2017051258A2 - Procédé de préparation d'engrais et composition de bactéries favorisant la croissance de plantes à ajouter à un engrais de base - Google Patents

Procédé de préparation d'engrais et composition de bactéries favorisant la croissance de plantes à ajouter à un engrais de base Download PDF

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Publication number
WO2017051258A2
WO2017051258A2 PCT/IB2016/001875 IB2016001875W WO2017051258A2 WO 2017051258 A2 WO2017051258 A2 WO 2017051258A2 IB 2016001875 W IB2016001875 W IB 2016001875W WO 2017051258 A2 WO2017051258 A2 WO 2017051258A2
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WO
WIPO (PCT)
Prior art keywords
bacteria
fertilizer
latent
pgpb
plant
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2016/001875
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English (en)
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WO2017051258A4 (fr
WO2017051258A3 (fr
Inventor
Francis COUTURE
Muhammad Arshad
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Doverfield Exports-Uae
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Doverfield Exports-Uae
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Publication date
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Priority to BR112017023724A priority Critical patent/BR112017023724A2/pt
Priority to MA41881A priority patent/MA41881B1/fr
Priority to CN201680029130.5A priority patent/CN107709274A/zh
Publication of WO2017051258A2 publication Critical patent/WO2017051258A2/fr
Publication of WO2017051258A3 publication Critical patent/WO2017051258A3/fr
Publication of WO2017051258A4 publication Critical patent/WO2017051258A4/fr
Anticipated expiration legal-status Critical
Priority to ZA2018/00768A priority patent/ZA201800768B/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02—Superphosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
    • C05C3/005—Post-treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C9/00—Fertilisers containing urea or urea compounds
    • C05C9/005—Post-treatment

Definitions

  • the present disclosure relates generally to fortified fertilizers and preparation methods thereof, especially mineral fertilizers fortified with plant growth promoting bacteria (PGPB).
  • PGPB plant growth promoting bacteria
  • Plant growth needs nutrients such as phosphorus (P), nitrogen (N), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), boron (B), copper (Cu), iron (Fe), manganese (Mn), molybdenum (Mo), and zinc (Zn).
  • these nutrients are typically introduced to the soil in form of synthetic/mineral fertilizers.
  • mineral fertilizers suffer from the problem of poor utilization by plant upon application to soil. For example, up to 60% of nitrogenous fertilizers applied to the soil could be lost through volatilization as NH 3 , leaching as NO 3 , or through denitrification.
  • up to 70-80%) of the applied amount could be fixed or precipitated in the soil matrix and cannot be readily absorbed by the crops.
  • PGPB plant growth-promoting bacteria
  • PGPB enhance plant growth through a variety of mechanisms, including fixation of atmospheric nitrogen, solubilization of minerals containing nutrient such as phosphorus, production of phytohormones, and siderophores, ACC deaminase, chitinase, and/or disease suppression.
  • bacteria in the groups of Bacilllus, and Pseudomonas are effective in solubilizing phosphorus.
  • Certain bacteria provide atmospheric N 2 to plant through symbiotic e.g. (rhizobia-legume) or asymbiotic (e.g. Azotobacter, Azospirillum & Clostridium) relationships.
  • Biofertilizers can be prepared by using previously tested effective microbes isolated from natural habitat, purified and multiplied in the presence of particular substrate to sustain high density of population. These biofertilizers are used as seed treatment, root inoculation or applied to soil to enhance the population of selected bacteria in the root zone which subsequently improves plant growth.
  • biofertilizers have not been widely used due to several limitations or constraints.
  • One of the constraints is that PGPB often need to be introduced via seed inoculation or fertigation, which could be both capital intensive and labor intensive.
  • Another constraint is that PGPB or biofertilizers often have limited shelf life and may lose potency shortly after being prepared.
  • storing PGPB or biofertilizers often requires special conditions, which limit their usage.
  • the present disclosure relates to process of preparation of high efficiency mineral fertilizers through fortification with specific plant growth promoting bacteria (PGPB), wherein the presence of novel PGPB on mineral fertilizers enhances fertilizer use efficiency.
  • PGPB plant growth promoting bacteria
  • the current disclosure also teaches a method for making a hybrid of mineral and biofertilizer for efficient use of both.
  • the method comprises the steps of culturing the selective bacterial strains, adjusting the microbiological culture to a temperature of about 25° C to about 45° C and a pH value of about 3.8 to about 9, and impregnating the microbiological culture onto a base fertilizer, e.g., a mineral fertilizer.
  • a base fertilizer e.g., a mineral fertilizer.
  • the microbiological culture is obtained by identifying bacteria to be included in the microbiological culture, purifying the bacteria, and fermenting the bacteria in the microbiological culture to reach a pre-determined density.
  • bacteria may contain enzyme 1-aminocyclopropane-l-carboxylate (ACC) deaminase, or to enhance phosphorous solubilization (i.e., P-solubilization), or enhance the use efficiency of nutrients released from mineral fertilizers.
  • ACC 1-aminocyclopropane-l-carboxylate
  • P-solubilization phosphorous solubilization
  • the bacteria in the microbiological culture is transformed into a latent (i.e., passive) state.
  • the latent bacteria can be reactivated upon provision of suitable conditions, either naturally such as in presence of root exudates from plant or by human manipulation such as in a lab or a factory.
  • the latent bacteria can be produced by exposing the bacteria to a controlled temperature at a certain acidity.
  • the medium where the bacteria grow can be adjusted to a temperature range of about 13° C to about 18° C and a pH range of about 5 to about 7.
  • the PGPB-fortified fertilizer comprises a base fertilizer and latent bacteria that increase the uptake of nutrients by a plant through mechanisms such as P-solubilization, ACC deaminase activity and/or slow release of nutrients resulting in less losses or relatively low insolubilization /fixation.
  • the base fertilizer may be a mineral fertilizer that comprises phosphorus, nitrogen, potassium, calcium, magnesium, sulfur, boron, copper, iron, manganese, molybdenum, zinc, and a mixture thereof.
  • the latent bacteria can be introduced to the base fertilizer by impregnation of PGPB onto a base fertilizer. Once the impregnated fertilizer is applied to the soil, the latent PGPB are reactivated in presence of exudates from a plant root. Alternatively, the PGPB-fortified fertilizer having the latent PGPB bacteria can be reactivated under controlled conditions in a processing facility such as a laboratory.
  • the current disclosure provides embodiments of fortifying mineral fertilizers with the latent phase PGPB carrying growth promoting traits such as P-solubilization and/or ACC deaminase activity.
  • PGPB-fortified fertilizers having applied to soil through standard methods, improve plant growth by enhancing nutrient bioavailability as well as promoting extensive root growth.
  • FIG. 1 illustrates a method of producing the PGPB-fortified fertilizer according to one embodiment of the current disclosure.
  • R RL+k*(RU-RL), wherein k is a variable ranging from 1% to 100% with a 1% increment, i.e., k is 1%, 2%, 3%, 4%, 5%, . . . , 50%, 51%, 52%, . . . , 95%, 96%, 97%, 98%, 99%, or 100%.
  • k is a variable ranging from 1% to 100% with a 1% increment, i.e., k is 1%, 2%, 3%, 4%, 5%, . . . , 50%, 51%, 52%, . . . , 95%, 96%, 97%, 98%, 99%, or 100%.
  • any numerical range defined by two R numbers as defined above is also specifically disclosed.
  • the drawing depicts a method of producing a high efficiency fertilizer through fortification by PGPB according to one embodiment of the current disclosure.
  • Candidate bacterial strains beneficial to plant growth are identified through rigorous screening and testing.
  • Candidate bacterial strains are selected from PGPB.
  • bacteria in the groups of Bacilllus and Pseudomonas are effective in solubilizing phosphorus.
  • Free-living bacteria that have nitrogen-fixation abilities include the Cyanobacteria (i.e., the blue-green algae) and some in the genus Clostridium, Azotobacter, or Azosparillum.
  • the selected PGPB strains are purified using standard procedures and transferred to a mother culture.
  • the mother culture is then subject to one or more steps of fermentation to increase the bacterial population density. Fermentation can be carried out in a bioreactor.
  • the conditions under which fermentation is carried out include the type of nutrients added to the culture, its temperature, its pH value, the speed of agitation, the time of incubation, the aeration rate, etc.
  • the microbial culture obtained after the fermentation step has a high bacterial density, e.g., about 10 7 cells to about 10 9 cells per milliliter. This fermentation step does not compromise the growth promoting traits, including P-solubilizing and ACC deaminase activities of the mother culture.
  • the bacteria in the microbial culture are converted into a latent state by adjusting the temperature (i.e., the temperature shock) and/or the pH value of the microbial culture (i.e., the pH shock).
  • the temperature of the microbial culture is adjusted to a value in the range of about 13° C to about 25° C, preferably at about 13° C to about 18°C.
  • the pH value of the microbial culture is adjusted to a value in the range of about 3.8 to about 9, or preferably about 5 to about 7. Consequently, the bacteria in the microbial culture become latent or inactive/passive temporarily.
  • the mother microbial culture experiences a controlled fermentation process to increase the population density and to enhance the growth promoting traits, including P-solubilizing and ACC deaminase activities.
  • the controlled fermentation process involving appropriate variables such as nutrients, temperature, pH, agitation, incubation, and aeration conditions, entail a series of fermentation steps.
  • Such a PGPB-fortified fertilizer can be easily transported and stored.
  • the latent PGPB it carries can be reactivated by the exudate released by plant roots.
  • the latent PGPB can be reactivated in a processing facility (e.g., a laboratory) under conducive culturing conditions.
  • the microbial culture containing latent PGPB is then added to a base fertilizer.
  • Suitable base fertilizers can be mineral fertilizers including, but not limited to, diammonium phosphate (DAP), single super phosphate (SSP), urea, calcium ammonium nitrate (CAN), etc.
  • DAP diammonium phosphate
  • SSP single super phosphate
  • CAN calcium ammonium nitrate
  • the microbial culture can be sprayed onto (or otherwise mixed with) the base fertilizer so that the surface of fertilizer is coated with the microbial culture.
  • the microbial culture can be added to the base fertilizer in a mixer. Accordingly, mixing a base fertilizer with the latent PGPB using any known methods produces a PGPB-fortified fertilizer of the current disclosure.
  • the PGPB-fortified fertilizer thus produced may contain both the mineral fertilizer as well as PGPB, which has a synergistic effect on plant growth and crop yields. Furthermore, the temperature shock and the pH shock can be carried out simultaneously or in sequence to convert the high density bacterial culture into a latent phase.
  • PGPB in the fortified fertilizer are in a latent phase, they may survive long term storage and harsh conditions encountered during transportation. Accordingly, the fortified fertilizers are suitable for long range transportation and long term storage.
  • the latent PGPB can be reactivated to recover ACC deaminase and P-solubilizing activity by exposure to root exudate or by processing under favorable controlled conditions, such as in a laboratory.
  • the fortified fertilizer of the current disclosure helps in achieving the food security by improving fertilizer use efficiency as well as by promoting crop yields due to the synergistic positive effects of specific PGPB and mineral fertilizer.
  • Example 1 Determining the PGPB in PGPB fortified fertilizer.
  • PGPB was isolated from the PGPB-fortified fertilizer and the result was compared with a control sample (i.e., a base mineral fertilizer without PGPB).
  • a control sample i.e., a base mineral fertilizer without PGPB.
  • PBS phosphate buffered saline
  • TLB Tryptic Soy Broth
  • inventive sample cultures and comparative sample cultures for testing were prepared.
  • inventive samples and comparative samples were added into separate phosphate buffered saline and incubated for 72 hours.
  • the resulting solutions were then poured on TSB agar plates, respectively. These plates were incubated at 30° C. After 24-48 hours, the bacterial growth in each plate was observed and the number of the bacteria was determined.
  • the sample from PGPB fortified fertilizer resulted in a bacterial density ranges from 10 to 10 cells per ml.
  • Bioassay Bioassays are used to qualitatively determine the growth promoting traits. Bioassays are considered the simplest and the most efficient methods to document the impact of PGPB on plant growth promotion under controlled conditions. Certain bioassays are highly specific and can be completed within few days.
  • ACC deaminase activities were determined quantitatively by measuring the amount of a-ketobutyrate produced when the enzyme ACC deaminase cleaves ammonia for ACC.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un procédé de préparation d'un engrais enrichi. Selon ce procédé, une forme latente de bactéries favorisant la croissance de plantes est supportée sur l'engrais de base. L'invention concerne également une composition de bactéries favorisant la croissance de plantes.
PCT/IB2016/001875 2015-07-07 2016-07-05 Procédé de préparation d'engrais et composition de bactéries favorisant la croissance de plantes à ajouter à un engrais de base Ceased WO2017051258A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
BR112017023724A BR112017023724A2 (pt) 2015-07-07 2016-07-05 método de preparação de um fertilizante e uma composição de bactérias promotora do crescimento de plantas para adicionar a um fertilizante base
MA41881A MA41881B1 (fr) 2015-07-07 2016-07-05 Procédé de préparation d'engrais et composition de bactéries favorisant la croissance de plantes à ajouter à un engrais de base
CN201680029130.5A CN107709274A (zh) 2015-07-07 2016-07-05 制备肥料的方法以及一种用于添加到基肥中的促进植物生长的细菌组合物
ZA2018/00768A ZA201800768B (en) 2015-07-07 2018-02-06 Method of preparing a fertilizer and a plant growth promoting bacteria composition for adding to a base fertilizer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
VN1-2015-02457 2015-07-07
VN1201502457 2015-07-07

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WO2017051258A2 true WO2017051258A2 (fr) 2017-03-30
WO2017051258A3 WO2017051258A3 (fr) 2017-06-08
WO2017051258A4 WO2017051258A4 (fr) 2017-08-24

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021016597A1 (fr) * 2019-07-24 2021-01-28 Shastri Parshuram Nirajan Complément minéral comprenant des microbes destinés à favoriser la croissance en agriculture
WO2023019258A1 (fr) * 2021-08-12 2023-02-16 The Regents Of The University Of California Compositions et procédés d'encapsulation par gel de silice de bactéries favorisant la croissance des plantes

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0768072B2 (ja) * 1985-12-27 1995-07-26 義明 木村 窒素固定菌を使用する有機肥料の製造方法
US8097280B2 (en) * 2003-07-24 2012-01-17 Evl Inc. Fertilizer and method for producing same
AU2006217568B8 (en) * 2005-02-22 2011-07-14 Evl Inc. Enhanced fertilizer and method for producing same
CA2921846C (fr) * 2013-08-19 2022-07-05 The Mosaic Company Systeme et procedes d'addition d'additifs agricoles, biologiques et/ou depoussierants avantageux a des engrais en granules
HUE048666T2 (hu) * 2013-09-04 2020-08-28 Indigo Ag Inc Mezõgazdasági endofita-növény kompozíciók és alkalmazási eljárások

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021016597A1 (fr) * 2019-07-24 2021-01-28 Shastri Parshuram Nirajan Complément minéral comprenant des microbes destinés à favoriser la croissance en agriculture
WO2023019258A1 (fr) * 2021-08-12 2023-02-16 The Regents Of The University Of California Compositions et procédés d'encapsulation par gel de silice de bactéries favorisant la croissance des plantes

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WO2017051258A4 (fr) 2017-08-24
WO2017051258A3 (fr) 2017-06-08

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