WO2017141063A1 - Microscope holographique numérique automatique muni d'une cellule à débit traversier - Google Patents

Microscope holographique numérique automatique muni d'une cellule à débit traversier Download PDF

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Publication number
WO2017141063A1
WO2017141063A1 PCT/HU2017/000009 HU2017000009W WO2017141063A1 WO 2017141063 A1 WO2017141063 A1 WO 2017141063A1 HU 2017000009 W HU2017000009 W HU 2017000009W WO 2017141063 A1 WO2017141063 A1 WO 2017141063A1
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WIPO (PCT)
Prior art keywords
measuring cell
sensor
control panel
cell
object space
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PCT/HU2017/000009
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English (en)
Inventor
Csaba ILCSIK
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Waterscope International Zrt
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Waterscope International Zrt
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Publication of WO2017141063A1 publication Critical patent/WO2017141063A1/fr
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G01N15/0227—Investigating particle size or size distribution by optical means using imaging; using holography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
    • G01N21/453—Holographic interferometry
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G01N15/0227—Investigating particle size or size distribution by optical means using imaging; using holography
    • G01N2015/0233—Investigating particle size or size distribution by optical means using imaging; using holography using holography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/144—Imaging characterised by its optical setup
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/0005—Adaptation of holography to specific applications
    • G03H2001/005—Adaptation of holography to specific applications in microscopy, e.g. digital holographic microscope [DHM]
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
    • G03H2001/0447—In-line recording arrangement

Definitions

  • the subject of the invention is a digital holographic automatic microscope mounted through flowing cell, which automatically produces three dimensional images about the liquid flowing through the cell, and by using image creating, image processing and particle sorting software it is capable of quasi-continuous sampling of the liquid flowing through it, or can be used for remote supervision or as a technological intervention element.
  • the fields of its application primarily include monitoring of water quality, performing food processing and other microbiological tests.
  • the detection or the control of the quantity of microscopic organisms in water is often required during the use of drinking water and other water or during sewage water treatment.
  • the samples are observed with traditional microscope visually.
  • This procedure requires lots of time and professional knowledge, however it is inaccurate especially in the case of rare samples, if, for instance, the limit in 1 litre water is maximum 5 algae or worms, then the procedure could be regarded as accurate only after 200,000 measurements in case of the usual sample volume of 3 - 5 ⁇ .
  • the situation could be improved by a method, when nutrient solution is added to the sample, and so the much higher concentration can be measured more conveniently after several days long propagation.
  • the Hungarian patent document HU 227 108 entitled "Procedure to testing microorganisms in solid, liquid and gaseous materials” makes known a solution, which allows an automatic measuring process by measuring the redox potential of the microorganisms in the sample, but the test requires a cumbersome preparation, also addition nutrient solution, and many hours of waiting is necessary.
  • the chlorophyll and other pigments of organisms in the water are measured based on the principle, that the chlorophyll starts to emit fluorescent light at 633 nm right after it is irradiated with 430 nm light, which can be measured easily with electronic instruments.
  • An illuminating light source is created after passing the beams of these light sources through optical fibres, and placing the light emitting ends close to one another.
  • the emitted light touches the object in the object space, and it gets divided into a reference beam and an object beam, and the interference image produced by them is recorded by a digital image detector, then the image can be divided into separate images for each focus plane with the help of know algorithms.
  • the equipment allows a high resolution, but its dimensional range is too narrow for measurements of the water quality, and the flow of sample in the object space is not really continuous, because it has to be stopped for the duration of the measurements.
  • the invention is based on the recognition, that the testing of the rare samples are frequent for checking the water quality, where the requirement could be 10 or even 1 pc/lit concentration regarding worms and algae. Actually, this cannot be checked in a proper manner in case of a single tested volume of 5 ⁇ on the average used with the traditional microscopy, but the volumetric measurement with holographic microscope can increase the speed of the measurement by magnitudes, if the formations found on the obtained images are compared to samples stored in database with the help of classicisation software, and a chart is made about their occurrence.
  • the measuring process can be automated easily, and it also allows an unattended remote measurement, and it offers the possibility of automatic technological intervention.
  • the high computational capacity can be located at other geographical location, because an internet link with suitable speed can be established at most of the measuring locations, and a higher calculating capacity can also be reached safely in cloud applications.
  • the invention is a digital holographic automatic microscope with through flowing cell, which has at least one power supply unit, a laser light source, a measuring cell, a digital camera.
  • An object space is formed within the measuring cell.
  • the object space, the camera and the laser are arranged along a single line.
  • the object space is bordered by at least two parallel transparent plates, the measuring cell has at least two liquid connectors, the liquid connectors are connected to the object space.
  • the measuring cell is supported by a cell console by means of detachable joint.
  • a measurement control panel and the power supply unit are connected to the camera, and to the light source.
  • the measurement control panel has a communication port and/or a communication unit.
  • a local computer is connected to the communication port / unit of the measurement control panel, and a display is connected to it, the computers has control and communication outlets also, image processing, classification and depicting programs run on the computer.
  • the measurement control panel is connected to a remote computer centre through its communication port / unit via wired or wireless link, where the image processing and classification programs run, and preferably at least one part of the computer centre is in cloud.
  • the material of the measuring cell is transparent.
  • the camera , the measuring cell and the light sources are mounted on a common, or more rails aligned in the same axis, or on console that can be adjusted linearly.
  • connection between the light source and the light source console is provide by at lest two elements such as screw-nut pair, the length of which can be adjusted.
  • a precision pump (31) is connected to at least one of the liquid connectors (20), which is connected to the measurement control panel (19).
  • an enlarger lens is connected to the camera, the light source is created by closely holding the light emitting ends of three optical fibres, and each optical fibre is connected to respective laser unit having different wave length.
  • the equipment according to the invention can be shaped so, that before or after the measuring cell in line or parallel according to the water flow with the measuring cell, there is one or more further a water quality monitoring sensors.
  • sensors can be e.g. pH sensor, temperature sensor, turbidity meter, conductivity sensor, dissolved oxygen sensor, ammonium sensor, nitrite sensor, nitrate sensor, permanganate index (Kolps) meter, dissolved/bound chlorine sensor, hydrogen sulphide sensor, orthophosphate and total phosphate sensor, UV conductivity sensor.
  • the sensors are connected to a common interface unit via serial communication, Mbus, RS485 channel.
  • Fig. 1 shows of the digital holographic automatic microscope with through flowing cell, which is applicable for automatic measurement in the field, when its door is open.
  • the Fig. 2 showes the equipment suitable for field measurement in also with axonometric view and while its door is open.
  • the Fig. 3 shows in cross section the measuring cells shown on Fig. 1-2, in side wiev.
  • the Fig. 4 shows in cross section the measuring cells shown on Fig. 1-2, in front wiev.
  • Fig. 5 shows the axonometric view of the measuring group of the equipment with increased resolution, including the transparent measuring cell.
  • Fig. 6 shows the axonometric view of the measuring group of the equipment with increased resolution, having a measuring cell with cover plate.
  • FIG. 7 The front view and a partial cross section of the measuring group of the equipment with increased resolution can be seen in Fig. 7 together with the measuring cell.
  • Fig. 8 shows the schematic diagram of the electronic units of the equipment.
  • Fig. 9 shows the measuring unit which is provided with sensors for monitoring the water quality.
  • Fig. 1 shows of the digital holographic automatic microscope with through flowing cell, which is applicable for automatic measurement in the field, when its door is open.
  • the shape of the housing, or the provision of doors or removable cover or other practical design is not prescribed strictly, but these items can ensure protection against the occurring environmental effects.
  • the display of the equipment 11 is installed in the door, which can be touch screen too.
  • the lower measuring section of the equipment and the upper computer section are separated from each other.
  • the computer section contains all the usual components of a general computer configuration: video card 13 (in the given case ASUS GTX660-DC2OCPH-2GD5 nVidia), power supply unit 14 (Chieftec GPS-400A8 400W PFC), hard disk 17, motherboard (GIGABYTE H61M-S2PV), processor (Intel 13), cooling fan 12.
  • the measurement control panel 19 can also be found here, which harmonizes the computer 15 and the operation of the digital camera 2, which is used with a source of coherent light, a laser 1 in the actual case, for producing the images.
  • the measuring section contains the units that directly participate in the measurement.
  • the figure shows the two water pipe connections or pipe outlet, the intake pipe 6 and the delivery pipe 10.
  • the intake pipe 6 is directly connected to a local water outlet point, which essentially provides continuous flow of water under pressure.
  • a local water outlet point which essentially provides continuous flow of water under pressure.
  • the flow rate below maximum 10 1/h in case of the equipment mentioned in the example, because the images could be blurred in case of higher speed.
  • the equipment not having a pump is installed, then the flow with suitable for proper velocity shall be ensured with the use of choke valves and pipe connections considering the local supply pressure.
  • an outlet for obtaining water with pressure is usually available. If no suitable outlet is available, then the sample still could be obtained if a small pump is installed.
  • the metered water is discharged through the delivery pipe 10.
  • the light source console 8 can be seen in Fig. 1, the distanced of which from the rear mounting plate can be adjusted with the screws 7.
  • the location of the laser 1 can be adjusted relative to the light source consol 8 in two directions perpendicularly to the axis of the laser 1 with the help of three position screws 9.
  • the possibility of adjusting the laser 1 is necessary, because the tolerance range is rather narrow regarding the image and position of the image produced at the sensor surface of the camera 2.
  • the Fig. 2 showes the equipment suitable for field measurement in also with axonometric view and while its door is open.
  • the cooling of the computer section is ensured by the fan 12.
  • the picture of the light source console 8 is partly broken out, thus the measuring cell 3 located behind it could be seen more conveniently.
  • the measuring cell 3 is a metal prism, which has a liquid connector 20 to the intake pipes, and one to the delivery pipe, wherein the two liquid connectors 20 are connected at the middle of the prism to the drilled object space 4 which is preferably aligned perpendicularly to the liquid connectors 20.
  • the measuring cell 3 is held by the counter plate 5 fixed to the rear mounting plate by means of screw in this case.
  • the front cover plate 26 is squeezed between the counter plate 5 and the measuring cell 3, or it is placed in the recess of the measuring cell 3, which ensures the transparency of the cell and the freedom from distortion necessary for the measurement.
  • the microscope cover plates can be ideally used for this purpose because of the parallel nature of their planes, their material and surfaces.
  • the type Epix SV10C6 can be ideally used for the purpose of the camera 2, which provides an image surface of 6.5 x 4.12 and a maximum resolution of 3840 x 2764.
  • the laser 1 is a 650 - 660 nm, 30 mW red laser, therefore, the image of the camera 2 is also monochrome.
  • This machine configuration allows the simultaneous production of interference images from significantly larger volumes (e.g. 1 cm3), as well as the detection and classification of particles and micro-organisms in the size range of 15 - 300 ⁇ .
  • the volume that can be tested in an hour may be up to 2 - 2.5 litres, and its limit is primarily determined by the computational capacity.
  • Fig. 3 and 4 show the sections in two directions of the measuring cell 3 introduced in the previous figures.
  • the section Fig. 3 showes is produced at one of the ends of the prism with lowered slicing.
  • the connecting passage of liquid connector 20 has a circular shape.
  • the threaded holes for the screw for supporting the counter plates 5 can be seen here.
  • the fixing of counter plates 5 with detachable joints can be accomplished with other solutions by professional people, the main function of the joints is to fix the measuring cell 3, and in the given case to hold the cover plates 26 as squeezed between the counter plate 5 and the measuring cell 3.
  • Figure 4 shows the object space 4, which preferably has a cylindrical shape, and it is linked to the liquid connector 20.
  • the cover plates could be formed so as to cover a side of the measuring cell 3 at a larger area, but they could also be held by the recesses aligned with circular symmetry in this case along the perimeter of the object space 4.
  • the function of the cover plate 26 is to border the object space 4 perpendicularly to the path of the laser beam, and also to hold the sample liquid inside.
  • Fig. 5 shows the axonometric view of the measuring group of the equipment with increased resolution, including the transparent measuring cell.
  • a lens 29 LUCPLFLN20x is actually placed on the camera 2 in order to ensure higher resolution, as a result of which the volume that can be measured is dramatically reduced to 1 mm3, however, the size range of the particles that can be measured is shifted to 3-100 ⁇ , with which the automatic classification of algae and fungi also becomes possible.
  • the light source in this case is provided by light emitting ends of three tightly unified optical fibres 30 with the help of three differently coloured lasers (red, green, blue) connected to the optical fibres 30.
  • the union of the three laser beams produces white light in the object space 4, therefore, colour images can also be produced.
  • the colour images combined with the high resolution allow, for example, the distinguishing between blue algae and green algae, as well as the detection of fungi.
  • the light source console 8, together with the cell console 24 and the camera consol, is mounted on a rail 23. At least two of the above consoles shall be fixed in a way, that they could be shifted along the axis of the rail 23. The possibility of shifting is necessary primarily for allowing fine tuning during production.
  • the camera 2, the object space 4 and the light source are located along a single line, which is an important feature in terms of arrangement. The above conditions may also be established by not placing the camera 2, the object space 4 and the light source on common rail, but by placing on other bracket allowing linear movement, or connected to brackets.
  • the camera console is fixed to the rail 23 by means of the camera consol screw 22, and preferably, the other consoles are also fixed to the rail 23 in a detachable manner.
  • the measuring cell 3 shown here has a compact design. Its material is transparent (e.g. silicate glass, borosilicate glass, polyester, etc.), therefore, it is not necessary to use separate cover plate or counter plate, and the measuring cell 3 can be fixed on the cell console 24 even directly, e.g. with the help of wing nuts shown in the figure. The possibility of assembling easily is beneficial because of the required periodic washing and replacement of cells.
  • the measuring cell 3 has two liquid connectors 20 similarly to the embodiment shown in the first two figures, with witch it is connected to a delivery pipe 6 and to an intake pipe 10.
  • the object space 4 is bordered here also necessarily perpendicularly to the light by means of (sheet-like) walls that consist of parallel surfaces.
  • the above requirement can also be met by placing sheet-like walls to the rest of the sides of the measuring cell 3 instead of having a cylindrical object space 4.
  • the measuring section shown in Fig. 5 may be placed in a common housing with the computer section and the measurement control panel 19 similarly to the device sown in Fig. 1 - 2, although the measuring section and the measurement control panel 19 (also including suitable power source or power supply unit) can also form a stand alone measuring unit 32 jointly, which is connected to a remote computer centre through its communication unit (e.g. modem), or through a wireless link, so that only the capture of the interference image takes place locally.
  • a remote computer centre through its communication unit (e.g. modem), or through a wireless link, so that only the capture of the interference image takes place locally.
  • the image processing and classicisation programs runs in a high capacity computer centre, which allows the increase of sampling frequency, and thus a larger sample volume is achieved, that can be actually tested, or even the entire volume flowing through the measuring cell 4 can be tested.
  • the obtained results are shown on the display 12 preferably in a graphic or other form.
  • At least part of the computer centre can run in a cloud as well, which allows the increase of available resources in a practical way, and in addition to that, the image processing and classification software are protected more efficiently against copying and reverse engineering.
  • the sample reservoir 21 which is not shown in the figures.
  • the depth of the object space is 0.8 - 2 mm, and because of the increased resolution caused by the lens 29, the testing of the moving sample is not sufficient any longer, because the image would be blurred, therefore, the flow has to be stopped before taking the pictures with the pump or with other flow control element, and it is necessary to wait a while until the liquid comes to equilibrium condition.
  • the sample reservoir 21 has a small size, and it can have a replaceable design too. As a consequence of the dimensions mentioned above, this high resolution machine using small samples is appropriate primarily among laboratory conditions.
  • Fig. 6 shows the axonometric view of the measuring group of the equipment with increased resolution, having a measuring cell with cover plate. Also, only the detail of the measuring section having larger resolution is shown in Fig. 6.
  • the connecting cable is not shown at the camera connector 25, but this cable is used to connect it to the measurement control panel 19.
  • the conceptual design of measuring cell 3 is close to the measuring cell shown in Fig. 2 - 4, but the one here is much thinner (while the object space is also 0.8 - 2 mm), and separate cover plates 26 are used to cover the opening of the object space 4.
  • the counter plate 5 and the cell console 24 together with the cover plate 26 are compressed by means of wing nuts and threaded bolts.
  • Fig. 7 shows the vertical section of the above arrangement in the axis of the object space 4.
  • the light source here is preferably provided also by three lasers with different colours, and the distance of the cell console 24 from the light source is adjusted in a way, that the union of the three beam with different colours is in the hole of the objects space 4, thus providing a white light illumination inside.
  • the cover plates 26 cover the hole of the object space 4 with overlap.
  • the delivery pipe 10 and the intake pipe 6 are connected to the object space 4 also through the liquid connector 20.
  • the materials of the measuring cell 3 in this case is preferably light metal. It can be seen in the figure, that the lens 29 and the object space 4 are aligned in the same axis, which is preferably parallel with the axis of the rail 23 too.
  • Fig. 8 shows the schematic diagram of the electronic units of the equipment.
  • the measurement control panel 19 consists of a micro controller, a USB I/O coupler, a JTAG I/O coupler and laser supply circuit(s).
  • the microcontroller provides the control signal to the connected camera 2 and laser(s) 1 synchronously.
  • the on time of the lasers 1 and the operation of the camera 2 shall be simultaneous, and even the laser 1 shall operate with suitable intensity during the time of exposure.
  • the measurement control panel 19 functions also as the driver of the laser.
  • the role of the communication port/unit is played by the USB I/O coupler in the given case, and it connects the computer 15 to the measurement control panel 19.
  • the measuring control panel 18 together with the camera 2, the optional pump 31 and laser 1 and communication port 28, and eventually a separate power supply unit, may operate as a stand alone measuring unit 32 also, if the communication port/unit 28 is connected to a remote computer centre via wired or wireless link, and this performs all the functions of the computer 15.
  • the Fig. 8 would be modified in a way, the units connected to the computer 15 as shown at the left, would be replaced with a remote computer centre, or with a cloud based service.
  • the computer 15 has a processor 16, a memory 18, a hard disk 17, and various devices are connected to it, such as the power supply unit 14, the display 11, the cooling fan 12 and the video card 13.
  • a further preferred feature of the equipment could be that the computer 15 is provided with controller and communication port 27.
  • This can be USB, LAN, WAN, VGA, or other standard port, as a result of which, the equipment can also function as an active element that intervenes in the associated technology, and at the same time, the full supervision of the measurement and classification can be accomplished even from large geographical distances.
  • the equipment By means the communication port, the equipment could function also as a command recipient device in the associated technological process.
  • Fig. 9 shows the measuring unit which is provided with sensors for monitoring the water quality. Only the measuring unit 32 is shown in Fig. 9, which is linked too a local computer 15 as mentioned above, or to a remote computer centre through the communication port 28. A version of the equipment is shown in the figure, wherein one or more of the sensors suitable for monitoring water quality are installed in series upstream or downstream to the measuring cell 3, or in a bypassing parallel water line.
  • temperature sensor 33 and turbidity meter 34 are installed in the pipe section between the intake pipe 6 and the measuring cell 3, and there is a nitrate sensor 37 in a branch parallel with the measuring cell 3, and in the water coming from the measuring cell 3 there is a pH sensor 35 and a dissolved oxygen sensor 36.
  • This configuration is only an example, and other devices may also be considered, such as flow meter, conductivity meter, UV conductivity sensor, conductivity sensor, dissolved oxygen sensor, ammonium sensor, nitrite sensor, permanganate index (Kolps) meter, dissolved/bound chlorine meter, hydrogen sulphide, orthophosphate and total phosphate sensor.
  • the sensors work with serial data transmission, and are connected preferably with Mbus, RS485 communication channel to the measurement control panel 19, which monitors their operation, and forwards the measured signals to the computer 15. Those sensors are necessary placed after or parallel with the measuring cell 3, which during the measuring their chemical or electrical effect influence the microscopic microorganisms to be detected.
  • the laser 1 and the camera 2 are operated simultaneously by the measurement control panel 19 with suitable frequency (e.g. after each 7 seconds).
  • the measurement control panel 19 stops the pump 31 before initiating the laser beam, then restarts the pump after the application of the leaser beam.
  • the sensor of the camera 2 takes an interference image, which depicts the entire depth of the object space 4.
  • the image processing software first inspects the image and selects the areas, where distortions indicate a high probability of the presence of some kind of microscopic particles at the given space section.
  • the device with the use of a classification software, is capable of comparing the images with the image database uploaded at the time of production, and to perform the analysis automatically. As a result, it can show, for example, simple diagrams or charts on the display 11 about the species and quantities of the found species.
  • the database uploaded by the factory shall include 50 - 100 images about the organisms and microorganisms to be detected. In order to be able to make the comparison many characteristics of the image are converted to measurable quantities (e.g.
  • the image database can be prepared or even extended by qualified personnel among laboratory conditions.
  • the measuring method is primarily suitable for rare samples, so that no particles covering one another could occur on the hologram, but with a little modification, e.g. by proper rarefaction of the sample it would be possible to test denser samples too.
  • the rarefaction could be made in the sample reservoir manually, but it could be made also by automatically mixing clear water with constant proportion in the delivery pipe 10 in case of an automatic field equipment which is constricted to stay at the water outlet.
  • the recommended equipment is capable of operation in fully automatic mode, while providing speeds exceeding that of the earlier monitoring procedure by magnitudes, and it is significantly more accurate too.
  • An additional outstanding feature is that it is capable of increasing the performance further if its version with remote computer centre is used, and it could be an active element also of the local technological process thanks to the available versatile external communication and control ports, and by using the fast interventions it could be used for operating much more efficient regulations (early warning system) relative to the former manual interventions.
  • Using an internet link it would be possible to poll the measuring results even in case of the version using local computer, so it is not necessary to have a microbiologist present at every measuring point.

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  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Le microscope holographique numérique automatique à cellule à débit traversier de l'invention comprend: au moins un bloc d'alimentation (14); une source de lumière laser (1); une cellule de mesure (3); une caméra numérique (2), un espace-objet (4) étant formé dans la cellule de mesure (3), l'espace-objet (4), la caméra (2) et le laser (1) étant disposés le long d'une ligne unique. Le microscope se caractérise en ce que l'espace-objet (4) est bordé d'au moins deux plaques transparentes parallèles et que la cellule de mesure (3) comporte au moins deux raccords de liquide (20). Les raccords de liquide (20) sont raccordés à l'espace-objet (4); la cellule de mesure (3) est portée par un support de cellule (24) au moyen d'un joint amovible; l'équipement comporte un panneau de commande de mesure (19) raccordé à la caméra (2) et au bloc d'alimentation (14); le panneau de commande de mesure (19) comporte un port de communication et/ou une unité de communication (28). Le microscope produit automatiquement des images tridimensionnelles du liquide s'écoulant à travers la cellule et, par la création d'images, le traitement d'images et un logiciel de tri de particules, il peut effectuer un échantillonnage quasi-continu du liquide s'écoulant à travers lui, ou peut être utilisé pour accomplir une supervision à distance ou comme élément d'intervention technologique. Ses domaines d'application comprennent principalement la surveillance de la qualité de l'eau, la transformation des aliments et d'autres tests microbiologiques.
PCT/HU2017/000009 2016-02-17 2017-02-09 Microscope holographique numérique automatique muni d'une cellule à débit traversier Ceased WO2017141063A1 (fr)

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