WO2017160104A3 - 응집형-형성 폴리펩타이드의 응집형을 검출하는 방법 - Google Patents

응집형-형성 폴리펩타이드의 응집형을 검출하는 방법 Download PDF

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Publication number
WO2017160104A3
WO2017160104A3 PCT/KR2017/002858 KR2017002858W WO2017160104A3 WO 2017160104 A3 WO2017160104 A3 WO 2017160104A3 KR 2017002858 W KR2017002858 W KR 2017002858W WO 2017160104 A3 WO2017160104 A3 WO 2017160104A3
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WO
WIPO (PCT)
Prior art keywords
aggregate
forming polypeptide
detecting
binding agent
tag
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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PCT/KR2017/002858
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English (en)
French (fr)
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WO2017160104A2 (ko
Inventor
이병섭
이관수
김신원
임군택
김광제
유지선
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PEOPLEBIO Inc
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PEOPLEBIO Inc
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Publication date
Application filed by PEOPLEBIO Inc filed Critical PEOPLEBIO Inc
Priority to JP2018548776A priority Critical patent/JP6745351B2/ja
Priority to ES17767005T priority patent/ES2956769T3/es
Priority to US16/085,070 priority patent/US11255863B2/en
Priority to CA3017723A priority patent/CA3017723C/en
Priority to EP17767005.6A priority patent/EP3431999B1/en
Priority to CN201780018756.0A priority patent/CN108885216B/zh
Priority to AU2017233132A priority patent/AU2017233132B2/en
Publication of WO2017160104A2 publication Critical patent/WO2017160104A2/ko
Publication of WO2017160104A3 publication Critical patent/WO2017160104A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4709—Amyloid plaque core protein
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2814—Dementia; Cognitive disorders
    • G01N2800/2821—Alzheimer

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 (a) 분석대상의 생시료에 상기 응집형-형성 폴리펩타이드의 다이머형을 스파이킹(spiking)하는 단계; (b) 상기 단계 (a)의 결과물을 인큐베이션 시켜 상기 응집형-형성 폴리펩타이드의 응집형을 추가적으로 형성시키는 단계; (c) 상기 단계 (b)의 결과물에 상기 응집형-형성 폴리펩타이드의 응집형에 결합하는 결합제에 시그널발생 표지가 결합된 결합제-표지를 접촉시키는 단계; 및 (d) 상기 응집형-형성 폴리펩타이드의 응집형에 결합된 결합제-표지로부터 발생되는 시그널을 검출하는 단계를 포함하는 생시료(biosample)의 응집형-형성 폴리펩타이드의 응집형을 검출하는 방법에 관한 것이다.
PCT/KR2017/002858 2016-03-16 2017-03-16 응집형-형성 폴리펩타이드의 응집형을 검출하는 방법 Ceased WO2017160104A2 (ko)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2018548776A JP6745351B2 (ja) 2016-03-16 2017-03-16 凝集型−形成ポリペプチドの凝集型を検出する方法
ES17767005T ES2956769T3 (es) 2016-03-16 2017-03-16 Procedimiento de detección de agregados de polipéptido formador de agregados
US16/085,070 US11255863B2 (en) 2016-03-16 2017-03-16 Method for detecting aggregate of aggregate-forming polypeptide
CA3017723A CA3017723C (en) 2016-03-16 2017-03-16 Method for detecting aggregate of aggregate-forming polypeptide
EP17767005.6A EP3431999B1 (en) 2016-03-16 2017-03-16 Method for detecting aggregate of aggregate-forming polypeptide
CN201780018756.0A CN108885216B (zh) 2016-03-16 2017-03-16 用于形成聚集体的多肽的聚集体检测方法
AU2017233132A AU2017233132B2 (en) 2016-03-16 2017-03-16 Method for detecting aggregate of aggregate-forming polypeptide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2016-0031534 2016-03-16
KR1020160031534A KR20170108203A (ko) 2016-03-16 2016-03-16 응집형-형성 폴리펩타이드의 응집형을 검출하는 방법

Publications (2)

Publication Number Publication Date
WO2017160104A2 WO2017160104A2 (ko) 2017-09-21
WO2017160104A3 true WO2017160104A3 (ko) 2018-08-09

Family

ID=59851066

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2017/002858 Ceased WO2017160104A2 (ko) 2016-03-16 2017-03-16 응집형-형성 폴리펩타이드의 응집형을 검출하는 방법

Country Status (10)

Country Link
US (1) US11255863B2 (ko)
EP (1) EP3431999B1 (ko)
JP (1) JP6745351B2 (ko)
KR (1) KR20170108203A (ko)
CN (1) CN108885216B (ko)
AU (1) AU2017233132B2 (ko)
CA (1) CA3017723C (ko)
ES (1) ES2956769T3 (ko)
TW (1) TWI698642B (ko)
WO (1) WO2017160104A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022098071A1 (ko) * 2020-11-03 2022-05-12 가톨릭대학교 산학협력단 구개편도선에서 알츠하이머병 유발 인자로서 베타아밀로이드 단백질 검출 및 이의 이용

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110081330A (ko) * 2006-04-21 2011-07-13 주식회사 피플바이오 삼차원적 상호작용을 이용하여 멀티머-형성 폴리펩타이드의 모노머로부터 멀티머를 분별 검출하는 방법
US8026070B2 (en) * 2005-02-19 2011-09-27 Peoplebio, Inc. Differential detection of multimeric and monomeric forms of multimer-forming polypeptides
KR101352849B1 (ko) * 2012-01-03 2014-01-21 주식회사 나노엔텍 멀티머-형성 폴리펩타이드의 멀티머형을 분별 검출하는 방법
KR20140069346A (ko) * 2011-10-04 2014-06-09 아피리스 아게 알츠하이머병 （ａｄ）을 진단하는 방법
KR20160067026A (ko) * 2014-12-02 2016-06-13 주식회사 피플바이오 응집형-형성 폴리펩타이드의 응집형을 검출하는 방법

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
CA1291031C (en) 1985-12-23 1991-10-22 Nikolaas C.J. De Jaeger Method for the detection of specific binding agents and their correspondingbindable substances
US4908305A (en) 1987-06-14 1990-03-13 The University Of Maryland Competitive elisa for determination of neutralizing IBDV antibody
IT1226712B (it) 1988-08-05 1991-02-05 Eniricerche Spa Metodo immunoenzimatico elisa monopiastra con inibizione competitiva per la rivelazione di anticorpi antisporozoitari di plasmodium falciparum.
US5143825A (en) 1990-03-01 1992-09-01 Abbott Laboratories Stabilized substrate for use in an immunoassay
SE9400088D0 (sv) 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US8658374B2 (en) * 2002-02-28 2014-02-25 Microsens Biphage Limited Binding of aggregated forms of proteins
CN101124342A (zh) 2005-02-19 2008-02-13 人民生物公司 示差检测多聚体形成的多肽的单体形式中多聚体形式的方法
WO2008124585A1 (en) * 2007-04-06 2008-10-16 Board Of Governors For Higher Education, State Of Rhode Island And Providence Planations Lowering of proteins associated with alzheimer's disease by interrupting gene transcription with a small molecule
CN101308144A (zh) 2007-05-16 2008-11-19 首都医科大学宣武医院 检测受试者体液内疾病相关蛋白聚合能力的方法
US20140213465A1 (en) * 2011-12-02 2014-07-31 Plaxgen, Inc. Plaque array methods and compositions for forming and detecting plaques
JP2013510297A (ja) * 2009-11-04 2013-03-21 ノバルティス アーゲー モノマーからのタンパク質凝集体の分離における結合試薬としての正に荷電した種
GB201101140D0 (en) * 2011-01-21 2011-03-09 Eisai Ltd Fused aminodihydrothiazine derivatives
EP2701743A4 (en) * 2011-04-27 2015-08-19 Univ Northwestern FOR PATHOLOGICAL TAU-DIMERE AND PREAFIBRILLARY PATHOLOGICAL TAU-OLIGOMER SELECTIVE ANTIBODIES AND THEIR USES IN THE APPLICATION, DIAGNOSIS AND MONITORING OF TAUOPATHIES
RU2514115C2 (ru) * 2011-12-01 2014-04-27 Общество с ограниченной ответственностью "Сенсорные системы" Устройство и способ для определения токсичности жидких сред
US9075069B2 (en) * 2011-12-13 2015-07-07 Baxter International Inc. Measurement of autoantibodies at low conductivity with increased sensitivity
KR102117701B1 (ko) 2013-07-12 2020-06-02 주식회사 피플바이오 혈액시료 내 멀티머-형성 폴리펩타이드의 모노머로부터 멀티머를 분별 검출하는 방법
US9618524B2 (en) * 2014-03-26 2017-04-11 Plaxgen Inc. Method, composition, isolation and identification of a plaque particle and related biomarker

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8026070B2 (en) * 2005-02-19 2011-09-27 Peoplebio, Inc. Differential detection of multimeric and monomeric forms of multimer-forming polypeptides
KR20110081330A (ko) * 2006-04-21 2011-07-13 주식회사 피플바이오 삼차원적 상호작용을 이용하여 멀티머-형성 폴리펩타이드의 모노머로부터 멀티머를 분별 검출하는 방법
KR20140069346A (ko) * 2011-10-04 2014-06-09 아피리스 아게 알츠하이머병 （ａｄ）을 진단하는 방법
KR101352849B1 (ko) * 2012-01-03 2014-01-21 주식회사 나노엔텍 멀티머-형성 폴리펩타이드의 멀티머형을 분별 검출하는 방법
KR20160067026A (ko) * 2014-12-02 2016-06-13 주식회사 피플바이오 응집형-형성 폴리펩타이드의 응집형을 검출하는 방법

Also Published As

Publication number Publication date
EP3431999A4 (en) 2020-01-01
CN108885216A (zh) 2018-11-23
TW201734457A (zh) 2017-10-01
CA3017723C (en) 2022-09-20
US11255863B2 (en) 2022-02-22
JP2019512700A (ja) 2019-05-16
EP3431999A2 (en) 2019-01-23
US20190120859A1 (en) 2019-04-25
EP3431999B1 (en) 2023-07-26
TWI698642B (zh) 2020-07-11
JP6745351B2 (ja) 2020-08-26
ES2956769T3 (es) 2023-12-27
WO2017160104A2 (ko) 2017-09-21
AU2017233132A1 (en) 2018-10-25
CA3017723A1 (en) 2017-09-21
EP3431999C0 (en) 2023-07-26
KR20170108203A (ko) 2017-09-27
CN108885216B (zh) 2022-03-22
AU2017233132B2 (en) 2021-02-25

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