WO2017160118A3 - 목적 단백질의 발현 효율을 증진시키기 위한 신규한 펩타이드 및 이를 포함하는 융합 단백질 - Google Patents

목적 단백질의 발현 효율을 증진시키기 위한 신규한 펩타이드 및 이를 포함하는 융합 단백질 Download PDF

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Publication number
WO2017160118A3
WO2017160118A3 PCT/KR2017/002902 KR2017002902W WO2017160118A3 WO 2017160118 A3 WO2017160118 A3 WO 2017160118A3 KR 2017002902 W KR2017002902 W KR 2017002902W WO 2017160118 A3 WO2017160118 A3 WO 2017160118A3
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WO
WIPO (PCT)
Prior art keywords
expression efficiency
target protein
novel peptide
including same
fusion protein
Prior art date
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Ceased
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PCT/KR2017/002902
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English (en)
French (fr)
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WO2017160118A2 (ko
Inventor
성백린
권순빈
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Inthera Inc
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Inthera Inc
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Publication date
Application filed by Inthera Inc filed Critical Inthera Inc
Priority claimed from KR1020170033692A external-priority patent/KR101915740B1/ko
Publication of WO2017160118A2 publication Critical patent/WO2017160118A2/ko
Publication of WO2017160118A3 publication Critical patent/WO2017160118A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 목적 단백질의 발현 효율을 증진시키기 위한 신규한 펩타이드 및 이를 포함하는 융합 단백질에 관한 것이다. 본 발명에 따른 신규한 펩타이드는 목적 단백질의 발현 효율을 향상시킬 수 있으며, 또한 상기 펩타이드를 포함하는 융합 단백질은 목적 단백질의 발현 효율뿐만 아니라 수용성을 향상시킴으로써, 재조합 목적 단백질의 생산에 유용하게 사용될 수 있다.
PCT/KR2017/002902 2016-03-18 2017-03-17 목적 단백질의 발현 효율을 증진시키기 위한 신규한 펩타이드 및 이를 포함하는 융합 단백질 Ceased WO2017160118A2 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20160032451 2016-03-18
KR10-2016-0032451 2016-03-18
KR10-2017-0033692 2017-03-17
KR1020170033692A KR101915740B1 (ko) 2016-03-18 2017-03-17 목적 단백질의 발현 효율을 증진시키기 위한 신규한 펩타이드 및 이를 포함하는 융합 단백질

Publications (2)

Publication Number Publication Date
WO2017160118A2 WO2017160118A2 (ko) 2017-09-21
WO2017160118A3 true WO2017160118A3 (ko) 2018-08-02

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PCT/KR2017/002902 Ceased WO2017160118A2 (ko) 2016-03-18 2017-03-17 목적 단백질의 발현 효율을 증진시키기 위한 신규한 펩타이드 및 이를 포함하는 융합 단백질

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WO (1) WO2017160118A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3696189A1 (en) * 2019-02-14 2020-08-19 European Molecular Biology Laboratory Means and methods for preparing engineered target proteins by genetic code expansion in a target protein selective manner

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040016654A (ko) * 2002-08-19 2004-02-25 프로테온 주식회사 Ｒｎａ 결합 단백질의 유전자를 융합파트너로 이용한재조합 단백질의 제조방법
KR20080094766A (ko) * 2007-12-24 2008-10-24 주식회사 벡손 새로운 융합파트너를 이용한 재조합 단백질의 제조방법
KR20130020206A (ko) * 2011-08-19 2013-02-27 연세대학교 산학협력단 수용성 ｂｍｐ-2를 생산하는 재조합 대장균 및 이를 이용한 수용성 ｂｍｐ-2의 제조방법

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040016654A (ko) * 2002-08-19 2004-02-25 프로테온 주식회사 Ｒｎａ 결합 단백질의 유전자를 융합파트너로 이용한재조합 단백질의 제조방법
KR20080094766A (ko) * 2007-12-24 2008-10-24 주식회사 벡손 새로운 융합파트너를 이용한 재조합 단백질의 제조방법
KR20130020206A (ko) * 2011-08-19 2013-02-27 연세대학교 산학협력단 수용성 ｂｍｐ-2를 생산하는 재조합 대장균 및 이를 이용한 수용성 ｂｍｐ-2의 제조방법

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHOI ET AL.: "Protein Solubility and Folding Enhancement by Interaction with RNA", PLOS ONE, vol. 3, no. 7, 16 July 2008 (2008-07-16), pages 1 - 10, XP055106439 *
PANG ET AL.: "tRNA Synthetase: tRNA Aminoacylation and Beyond", WILEY INTERDISCIPLINARY REVIEWS: RNA, vol. 5, July 2014 (2014-07-01), pages 461 - 480, XP055522725 *

Also Published As

Publication number Publication date
WO2017160118A2 (ko) 2017-09-21

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