WO2017188669A3 - 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물 - Google Patents

절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물 Download PDF

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WO2017188669A3
WO2017188669A3 PCT/KR2017/004297 KR2017004297W WO2017188669A3 WO 2017188669 A3 WO2017188669 A3 WO 2017188669A3 KR 2017004297 W KR2017004297 W KR 2017004297W WO 2017188669 A3 WO2017188669 A3 WO 2017188669A3
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sequence
nucleic acid
cleaved
complementary
primer
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WO2017188669A2 (ko
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김수옥
김석준
홍선표
정현재
조우재
김재일
양승민
조애리
홍성수
김정우
정선영
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Genematrix Inc
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Genematrix Inc
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Priority to CN201780025886.7A priority Critical patent/CN109072305A/zh
Priority to JP2018556900A priority patent/JP6796660B2/ja
Priority to EP17789853.3A priority patent/EP3453768B1/en
Priority to US16/095,695 priority patent/US11193161B2/en
Publication of WO2017188669A2 publication Critical patent/WO2017188669A2/ko
Publication of WO2017188669A3 publication Critical patent/WO2017188669A3/ko
Anticipated expiration legal-status Critical
Priority to US17/115,740 priority patent/US20210189456A1/en
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Abstract

본 발명은 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물에 관한 것으로, 구체적으로 PCR 프라이머에 표지 상보적 서열을 연결하여 PCR 반응 중 제한효소에 의해 표식이 생성되도록 하며, 각 프라이머에 연결하는 표지 상보적 서열을 길이 및 핵산의 조합등의 요소를 활용하여 다양화하고, 이를 이용해 표적 서열을 구분하는 방법에 관한 것이다. 본 발명에 따르면 엄격한 조건하에서 Cleaved Complementary Tag Fragment (CCTF)는 프라이머에 연결된 5' 말단의 임의 서열과 상보적 서열로서 PCR 반응과 제한효소 반응이 일어나지 않으면 형성되지 않으며 표적 서열에 혼성화 및 표적 서열과 상보적인 프라이머 신장 산물을 형성한 경우에서만 절단된 단일가닥이 형성되어 이를 판독함으로서 보다 높은 정확성을 확보할 수 있다. 또한 CCTF는 다양한 분석기법 및 분석장비를 사용자 의도에 맞게 선택하여 다수의 표적 핵산 서열을 동정하는데 활용 할 수 있다. 이를테면 유전자 검사, 샘플 내의 생물체 동정, 미생물 또는 바이러스의 감염 여부 진단 등에서 신속하고 정확하게 결과를 확인할 수 있다.
PCT/KR2017/004297 2016-04-25 2017-04-21 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물 Ceased WO2017188669A2 (ko)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN201780025886.7A CN109072305A (zh) 2016-04-25 2017-04-21 利用切割的互补标签片段的靶核酸序列检测方法及其组合物
JP2018556900A JP6796660B2 (ja) 2016-04-25 2017-04-21 切断された相補的なタグ切片を用いた標的核酸配列の検出方法及びその組成物
EP17789853.3A EP3453768B1 (en) 2016-04-25 2017-04-21 Method for detecting target nucleic acid sequence using cleaved complementary tag fragment and composition thereof
US16/095,695 US11193161B2 (en) 2016-04-25 2017-04-21 Method for detecting target nucleic acid sequence using cleaved complementary tag fragment and a composition therefor
US17/115,740 US20210189456A1 (en) 2016-04-25 2020-12-08 Method for detecting target nucleic acid sequence using cleaved complementary tag fragment and a composition therefor

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KR10-2016-0050313 2016-04-25
KR20160050313 2016-04-25

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US16/095,695 A-371-Of-International US11193161B2 (en) 2016-04-25 2017-04-21 Method for detecting target nucleic acid sequence using cleaved complementary tag fragment and a composition therefor
US17/115,740 Division US20210189456A1 (en) 2016-04-25 2020-12-08 Method for detecting target nucleic acid sequence using cleaved complementary tag fragment and a composition therefor

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WO2017188669A2 WO2017188669A2 (ko) 2017-11-02
WO2017188669A3 true WO2017188669A3 (ko) 2018-08-09

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US (2) US11193161B2 (ko)
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KR (1) KR101961642B1 (ko)
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KR102380264B1 (ko) 2017-08-31 2022-03-29 주식회사 씨젠 다이머-형성 프라이머 쌍을 이용한 구성요소의 성능 평가
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
EP3735476B1 (en) 2018-01-05 2023-08-23 Seegene, Inc. Method for determining the presence or absence of m. tuberculosis, m. bovis and m. bovis bcg in a sample
EP3781708B1 (en) 2018-04-20 2025-01-15 Seegene, Inc. Method for detecting a plurality of target nucleic acid sequences in sample
CN110702813B (zh) * 2019-10-23 2022-03-11 贵州中医药大学 一种苗药黑骨藤hplc指纹图谱研究及多成分含量测定方法
CN111041127A (zh) * 2020-01-10 2020-04-21 上海润达榕嘉生物科技有限公司 一种hsv1病毒的检测引物及其试剂盒
ES3034761T3 (en) * 2020-10-29 2025-08-22 Biocartis Nv Generic cartridge and method for multiplex nucleic acid detection
JP7585843B2 (ja) * 2021-02-10 2024-11-19 株式会社島津製作所 オリゴヌクレオチドプローブ、検査液および検出方法
CN115873946A (zh) * 2022-08-04 2023-03-31 山东鲁抗好丽友生物技术开发有限公司 基于Blocker的基因突变检测试剂盒及其应用

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