WO2017196077A1 - Additif cosmétique et nutritionnel utilisant un aptamère et procédé pour le produire - Google Patents

Additif cosmétique et nutritionnel utilisant un aptamère et procédé pour le produire Download PDF

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Publication number
WO2017196077A1
WO2017196077A1 PCT/KR2017/004823 KR2017004823W WO2017196077A1 WO 2017196077 A1 WO2017196077 A1 WO 2017196077A1 KR 2017004823 W KR2017004823 W KR 2017004823W WO 2017196077 A1 WO2017196077 A1 WO 2017196077A1
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WO
WIPO (PCT)
Prior art keywords
aptamer
vitamin
antioxidant
extract
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2017/004823
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English (en)
Korean (ko)
Inventor
손인식
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nexmos Co Ltd
Original Assignee
Nexmos Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nexmos Co Ltd filed Critical Nexmos Co Ltd
Publication of WO2017196077A1 publication Critical patent/WO2017196077A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/13—Nucleic acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides

Definitions

  • the present invention relates to cosmetics and nutritional supplements using aptamers and to a method of manufacturing the same, and more particularly, to cosmetics and nutritional supplements using long-term maintenance and antioxidant properties of antioxidants such as reduced vitamin C by aptamers. Is to apply.
  • ROS active oxygen species
  • Antioxidants can be divided into those that exist naturally in the human body and those that are administered externally.
  • Antioxidants that are naturally present in the human body include enzymes such as superoxide dismutase (SOD), glutathione, peroxidase and catalase. phytochemicals such as kaempferol, catechin and genistein; Vitamin E, vitamin C and beta carotene; And minerals such as selenium.
  • SOD superoxide dismutase
  • glutathione glutathione
  • peroxidase and catalase
  • phytochemicals such as kaempferol, catechin and genistein
  • Vitamin E Vitamin C and beta carotene
  • minerals such as selenium.
  • Vitamin C L-ascorbic acid, ascorbate
  • Vitamin C deficiency will cause diseases such as scurvy (Scurvy).
  • Vitamin C in animals is an important factor in preventing wound healing and capillary bleeding, and is an important antioxidant in the body along with Vitamin A and Vitamin E.
  • UVA UVA
  • UVB ultraviolet B
  • pollutants pollutants
  • stress smoking, drinking, fatty foods, etc.
  • cells may age or die.
  • these substances reduce or degenerate the production of collagen and elastin, causing the skin to lose elasticity and wrinkles.
  • antioxidants such as vitamin A, C, E
  • the present invention has been made in view of the above necessity, and an object of the present invention is to provide a substance which acts as an antioxidant for antioxidant substances.
  • the present invention provides an aptamer for preventing oxidation of an antioxidant substance.
  • the present invention also provides an aptamer and an antioxidant complex obtained by combining an antioxidant with the aptamer of the present invention.
  • one example of the aptamer is preferably composed of the nucleotide sequence set forth in SEQ ID NO: 1, in addition to the aptamer has a different sequence as demonstrated by the examples of the present invention, etc. All aptamers that achieve the desired effect of are included in the scope of protection of the present invention.
  • the antioxidant is preferably a substance selected from the group consisting of vitamin C, vitamin A, retinol, vitamin E, astaxanthin, resveratinol, polyphenols, coenzyme Q10, peptides and oils.
  • the antioxidant is not limited thereto.
  • the present invention provides a cosmetic composition comprising the aptamer and antioxidant complex of the present invention as an active ingredient.
  • the cosmetic composition preferably further comprises one or more of collagen, elastin, hyaluronic acid, and a peptide, but is not limited thereto.
  • the cosmetic composition is to release the bound antioxidant (eg, vitamin C) when the structure of the aptamer changes according to the ultraviolet irradiation of the skin, or ATP according to the change in the temperature or acidity of the skin It is preferable to release vitamin C as the structure of the aptamer which binds thereto when the amount is changed, but is not limited thereto.
  • the bound antioxidant eg, vitamin C
  • the cosmetic composition of the present invention can use any raw material used in cosmetics regardless of any kind of extracts, active substances.
  • green tea extract, licorice extract, medicinal extract, lettuce extract, golden extract, pueraria extract, red ginseng extract and apricot extract oil extract, orange extract, lemon extract, bamboo extract guava extract Rosemary Extract Cornus Extract, Ganoderma Lucidum Extract Ginkgo Extract Seoxiok-Yuksan Extract Consonant Extract, Moisturizing Chinese Quince Extract, White Lotus Extract, Paprika Extract, Aloe Extract, Scrubber Extract, Seaweed Extract, Antioxidant Carrot Extract, Soybean Extract, Grapefruit Extract Grape Seed Extract, Portulaca Extract, Caviar to Improve Wrinkles, Pomegranate, Ginseng Extract, Peach Extract for Skin Regeneration, Cheongung Extract, Centella asiatic Extract, Chamomile Extract, Licorice Extract, Ginseng Extract, Angelica Extract, Acne Good peppermint extract, triticale extract, eosungcho extract , Pe
  • the present invention provides a nutritional supplement composition comprising the aptamer and antioxidant complex of the present invention as an active ingredient.
  • vitamins for the nutritional supplements of the present invention include vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, vitamin B 6, vitamin B 12, thiamine, riboflavin, biotin, folic acid, niacin, pantothenic acid, mixtures thereof, and the like. to be.
  • suitable mineral nutrients to be included in the dietary supplement composition are selected from sodium, potassium, calcium, magnesium, phosphorus, sulfur, chlorine, iron, copper, iodine, zinc, selenium, manganese, chromium, molybdenum, fluorine, cobalt, and compounds thereof They hold more than one element.
  • herbs can also be used as nutritional supplements.
  • herbs are selected from those having various medicinal or dietary supplement properties.
  • herbs are aromatic plants or parts of plants that can be used for medicinal or flavoring purposes.
  • the present invention provides a method for binding an aptamer to an antioxidant to maintain the reduced state of the antioxidant and prevent oxidation.
  • the present invention like Vitamin C, vitamin A (Retinol), Vitamin E, Astaxanthin, Resveratinol, Polyphenol, Coenzyme Q10, Peptide, Oil, etc. Can be used to manufacture. This prevents the oxidation (corrosion) of the materials to ensure that they maintain the maximum effect they require. In addition, by releasing it under a target condition through apta-sensing, the effect can be increased as much as possible.
  • Aptamer is a method of detecting a specific substance by using the three-dimensional structure of single strand DNA or RNA, which is similar to an antigen-antibody reaction, but has a much smaller substance, and can be controlled in various ways. This is an easy advantage. In addition, unlike antibodies, it is possible to synthesize aptamers that bind to very small chemicals (vitamins) and the like.
  • Vitamin C is a water-soluble six carbon compound, which includes the 3-, 4- and 3-hydrocarbons of the furan ring and the semidehydroascorbic acid and dehydroascorbic acid, which are oxidized in the reduced form and biparticulate, respectively.
  • Vitamin C which is combined with aptamers and maintains a reduced state, may be used in skin care compositions and nutritional supplements of various types of cream or hydrogel types including collagen, elastin, hyaluronic acid and peptides.
  • the present invention also includes a method of slowly releasing Vitamin C according to various conditions of the skin (Aptasensing) through aptamers that react differently depending on the condition of the skin or external stimuli (eg ultraviolet rays or the temperature or acidity of the skin). For example, when the structure of the aptamer is changed by UV irradiation of the skin, the bound antioxidant (e.g., vitamin C) is released or the structure of the aptamer that binds when the amount of ATP changes according to the temperature or acidity of the skin is changed. And a method of releasing Vitamin C as a substitute.
  • aptamers that react differently depending on the condition of the skin or external stimuli (eg ultraviolet rays or the temperature or acidity of the skin). For example, when the structure of the aptamer is changed by UV irradiation of the skin, the bound antioxidant (e.g., vitamin C) is released or the structure of the aptamer that binds when the amount of ATP changes according to the temperature or
  • the aptamer of the present invention has an antioxidant effect of an antioxidant substance such as vitamin C, and the complex of the aptamer and antioxidant of the present invention may be, for example, contained in Vitamin C (ascorbic acid).
  • Vitamin C ascorbic acid
  • Aptamers that selectively bind to maintain the reduced state of Vitamin C to maintain its antioxidant function for a long time can be used in functional cosmetics and oral dietary supplements of various formulations.
  • RNA or DNA RNA
  • Figures 2 to 4 show that the three aptamers of the present invention prevent vitamin C oxidation by hydrogen peroxide solution
  • 5 to 10 are graphs of dissociation constants (KD) for AA (ascorbic acid) and DHA (dehydroascorbic acid) of the aptamer of the present invention.
  • 11 is a structure and sequence information of the aptamer used in the present invention.
  • Example 1 bound to reduced vitamin C Aptamer Military building
  • SELEX systematic evolution of ligands by exponential enrichment
  • aptamers were selected and subjected to the anti-oxidation experiment of vitamin C. After heating the aptamer dissolved in the annealing buffer to 95 o C and slowly lowering the temperature to room temperature, the secondary structure of the aptamer is mixed. The reaction was carried out for about 30 minutes. After oxidizing conditions by adding hydrogen peroxide solution, oxidation of L-ascorbic acid was measured by adding OPDA (o-phenylenediamine) as a fluorescent dye. DHA, the oxide of L-ascorbic acid, can be quantitatively analyzed by measuring the amount of fluorescence from DHA-OPDA produced by reaction with OPDA. Under the above conditions, the amount of fluorescence of DHA-OPDA was measured every 34 seconds for 25 minutes.
  • OPDA o-phenylenediamine
  • Example 3 of the present invention Aptamer ascorbic acid (AA) and dehydroascorbic acid ( DHA Steady-state solution dissociation constant (KD)
  • Dissociation constants were determined using microscale thermophoresis (MST).
  • This example includes the MST data and the calculated KD for the three aptamers in the assay buffer for both targets.
  • All reagents containing ascorbic acid and dehydroascorbic acid were purchased from Sigma-Aldrich (St. Louis, MO) and deionized water was treated with Chelex-100 resin for 1 hour to remove accidental metals prior to buffer preparation. After Chelex treatment, the water was sprayed with nitrogen gas for 10 minutes to minimize oxygen and then sealed and stored. This water was used for all aqueous solutions.
  • Final buffer is 50 mM sodium acetate, pH 5.5, 1 mM MgCl 2, and 0.05% Tween-20, both AA and DHA were 153 pM (final) at e5 uM in buffer (for aptamers # 10 &# 12) Or at 1: 1 passage dilution at 1.53 nM (final) (Aptamer # 8) at 50 uM.
  • the final concentration of each Cy5-conjugated aptamer is 20 nM.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Cosmetics (AREA)

Abstract

La présente invention concerne un additif cosmétique et nutritionnel utilisant un aptamère et un procédé pour le produire. Plus spécifiquement, l'aptamère selon la présente invention a pour effet de prévenir l'oxydation d'un antioxydant, tel que la vitamine C. Le composite d'un aptamère et d'un antioxydant selon la présente invention utilise un aptamère se liant sélectivement à la vitamine C (acide ascorbique) par exemple, de façon à maintenir l'état réduit de la vitamine C et à maintenir ainsi sa fonction anti-oxydante pendant une longue période de temps, et peut donc être utilisée dans des cosmétiques fonctionnels de diverses formulations, des additifs nutritionnels oraux (compléments alimentaires) et des produits similaires.
PCT/KR2017/004823 2016-05-12 2017-05-10 Additif cosmétique et nutritionnel utilisant un aptamère et procédé pour le produire Ceased WO2017196077A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2016-0058022 2016-05-12
KR20160058022 2016-05-12
KRPCT/KR2016/011741 2016-10-19
PCT/KR2016/011741 WO2017195951A1 (fr) 2016-05-12 2016-10-19 Supplément cosmétique et nutritionnel utilisant un aptamère et procédé de production associé

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PCT/KR2017/004823 Ceased WO2017196077A1 (fr) 2016-05-12 2017-05-10 Additif cosmétique et nutritionnel utilisant un aptamère et procédé pour le produire

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108543079A (zh) * 2018-05-22 2018-09-18 广东药科大学 一种虾青素-胶原蛋白耦合物及其制备方法和应用
WO2020085805A1 (fr) * 2018-10-25 2020-04-30 주식회사 넥스모스 Composition pour soulager et traiter la perte de cheveux, les lésions des cheveux et la maladie de la peau d'un animal, comprenant un aptamère se liant a la vitamine c en tant que principe actif
WO2020096240A1 (fr) * 2018-11-06 2020-05-14 주식회사 넥스모스 Aptamère d'adn se liant de manière spécifique au glutathion, et utilisation associée
WO2020222506A1 (fr) * 2019-05-02 2020-11-05 주식회사 넥스모스 Composition orale comprenant un aptamère en tant que principe actif

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020096296A1 (fr) * 2018-11-06 2020-05-14 주식회사 넥스모스 Procédé pour empêcher l'oxydation de polyphenol à l'aide d'un aptamère, matériau correspondant et utilisation correspondante

Citations (4)

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Publication number Priority date Publication date Assignee Title
US6235721B1 (en) * 1999-02-17 2001-05-22 Natreon Inc. Stabilization of vitamin C
US20070112064A1 (en) * 2003-07-24 2007-05-17 Lyles Mark B Nucleic acid antioxidant compositions, methods for obtaining such compositions and formulations thereof
WO2009031788A1 (fr) * 2007-09-04 2009-03-12 Cotde, Ltd. Composition cosmétique comprenant de la vitamine c
KR20120043167A (ko) * 2009-04-09 2012-05-03 시므라이즈 아게 피부 자극-완화제로서 트랜스-텔트-부틸 사이클로헥사놀을 포함하는 조성물

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6235721B1 (en) * 1999-02-17 2001-05-22 Natreon Inc. Stabilization of vitamin C
US20070112064A1 (en) * 2003-07-24 2007-05-17 Lyles Mark B Nucleic acid antioxidant compositions, methods for obtaining such compositions and formulations thereof
WO2009031788A1 (fr) * 2007-09-04 2009-03-12 Cotde, Ltd. Composition cosmétique comprenant de la vitamine c
KR20120043167A (ko) * 2009-04-09 2012-05-03 시므라이즈 아게 피부 자극-완화제로서 트랜스-텔트-부틸 사이클로헥사놀을 포함하는 조성물

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HE, XIOAXIAO ET AL.: "ATP-responsive Controlled Release System Using Aptamer-functionalized Mesoporous Silica Nanoparticles", LANGMUIR, vol. 28, no. 35, 14 August 2012 (2012-08-14), pages 12909 - 12915, XP055106436 *
KIM, TAE-HWAN: "Nexmos, Targeting Global Anti-aging with Recombinant DNA Molecule", NEWS, 28 December 2016 (2016-12-28), pages 1 - 2, Retrieved from the Internet <URL:http://news1.kr/articles/?2870593> *
KRYLOVA, SVETLANA M. ET AL.: "Mechanistic Studies on the Application of DNA Aptamers as Inhibitors of 2-oxoglularate-dependent Oxygenases", JOURNAL OF MEDICINAL CHEMISTRY, vol. 55, no. 7, 3 April 2012 (2012-04-03), pages 3546 - 3552, XP055414197 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108543079A (zh) * 2018-05-22 2018-09-18 广东药科大学 一种虾青素-胶原蛋白耦合物及其制备方法和应用
WO2020085805A1 (fr) * 2018-10-25 2020-04-30 주식회사 넥스모스 Composition pour soulager et traiter la perte de cheveux, les lésions des cheveux et la maladie de la peau d'un animal, comprenant un aptamère se liant a la vitamine c en tant que principe actif
WO2020096240A1 (fr) * 2018-11-06 2020-05-14 주식회사 넥스모스 Aptamère d'adn se liant de manière spécifique au glutathion, et utilisation associée
WO2020222506A1 (fr) * 2019-05-02 2020-11-05 주식회사 넥스모스 Composition orale comprenant un aptamère en tant que principe actif

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