WO2017200340A2 - Peptide de collagène de faible poids moléculaire et procédé de préparation d'une composition alimentaire à l'aide de celui-ci - Google Patents

Peptide de collagène de faible poids moléculaire et procédé de préparation d'une composition alimentaire à l'aide de celui-ci Download PDF

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WO2017200340A2
WO2017200340A2 PCT/KR2017/005232 KR2017005232W WO2017200340A2 WO 2017200340 A2 WO2017200340 A2 WO 2017200340A2 KR 2017005232 W KR2017005232 W KR 2017005232W WO 2017200340 A2 WO2017200340 A2 WO 2017200340A2
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collagen
low molecular
preparing
peptide
low
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WO2017200340A3 (fr
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김구환
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Missarkbio Co Ltd
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Missarkbio Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/20—Shaping or working-up of animal feeding-stuffs by moulding, e.g. making cakes or briquettes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
    • A23K50/42—Dry feed

Definitions

  • the present invention relates to a low molecular collagen peptide and a method for producing a food composition using the same, and more particularly, a method for producing a low molecular collagen peptide utilizing a weak acid containing hydrogen calcium and a companion containing the low molecular collagen peptide prepared by the method It relates to a method for producing a composition for animal nutrition.
  • Collagen is a protein found in most animals and is a major protein that makes up the flesh and connective tissue of mammals in particular. It is rich in 25% to 35% of the proteins that make up the body.
  • the increased fibrous collagen is mainly present in fibrous tissues such as tendons, ligaments and skin, and also exists in the cornea, cartilage, bone, blood vessels, digestive tract, and spinal disc.
  • Collagen acts as a major component of the endocardium.
  • Collagen has a molecular weight of 300,000 and three ⁇ -molecules with a molecular weight of 100,000 form a ⁇ -ray. It is a triple helix structure of 1.5 nm in diameter and 300 nm in length. ⁇ chains are strongly bonded by some hydrogen bonds, and when microfibrils are gathered, they become collagen fibrils by covalent cross-linking, and they become fibers.
  • Collagen like other proteins, the structure, synthesis amount, etc. are determined according to the genetic information. Molecular biology and biochemistry studies on collagen have recently made significant advances and their structure has been elucidated in detail. Searching the literature on the topic of collagen reveals that more than 10,000 publications are published annually. To date, more than 13 types have been reported, depending on the type of molecule, and more than 22 types of polypeptide chains. In general, the difference with other proteins is that collagen protein does not function as a single molecule, and only plays a role in the living body when many molecules are combined to form a higher structure.
  • Collagen is also a protein that is the largest quantitative and functional part of the human body, meaning the extracellular matrix, more specifically the major constituent of connective tissue. do.
  • Human tissues containing large amounts of collagen include bone tissue such as skin, bone and cartilage, tendons, ligaments, and the like. Aging of such tissues causes human aging diseases such as wrinkles and sagging skin, and osteoporosis, which is known to be due to a decrease in the amount and function of collagen in the body due to a decrease in collagen synthesis of tissues.
  • a feature of the collagen amino acid composition is that glycine makes up about one third of the total.
  • 2/9 of the whole is composed of proline or hydroxyproline (Hydroxy-proline). Hydroxyproline is produced when proline on the translational peptide of the collagen gene is hydroxylated at position 4 carbon by molecular oxygen.
  • the content of amino acids depends on the type of animal, especially the upper limit of the body's body temperature, and 2/9 is the case of constant temperature animals such as birds and mammals. That is, the thermal stability of the collagen structure depends on the imino acid content, in particular on the amount of hydroxyproline.
  • Hydroxyproline is rarely found in proteins other than collagen (somewhat present in elastin), and the amount of collagen can be measured by quantifying hydroxyproline, since only proline contained in the peptide is hydrated to form hydroxyproline. (Hyproproline content is about 10%, so 100 ⁇ g of hydroproline, about 1 mg as collagen).
  • hydroxyproline is used as an indicator of the total amount of collagen protein synthesis, the amount of degradation and the like.
  • the hydroxyproline content in collagen varies not only with the form (gene) of collagen, but also with the conditions (environmental conditions) when animal tissues and cells synthesize.
  • the amount of lysine and arginine is higher than that of glutamic acid or aspartic acid, and the charge of the whole collagen peptide has a positive charge in neutral pH. That is, the isoelectric point can be seen as basic. More glycine and less hydrophobic amino acids result in a smaller volume of molecules (0.75 for globular proteins, about 0.70 for collagen), and an average amino acid molecular weight of less than 100 (for globular proteins, usually 110).
  • the amount of collagen produced decreases with age. Collagen production is the highest in 20s, and 70% is known to decrease rapidly afterwards. Therefore, collagen is not a substance produced in the body, so it is necessary to take it as food.
  • Collagen can be divided into high molecular collagen and low molecular collagen according to the molecular weight.
  • Polymer collagen generally has a molecular weight of 5,000 Da.
  • polymer collagen with a molecular weight of 5,000 or more is used for general foods and the like.
  • low molecular weight collagen has a molecular weight of 1,500 Da. The following things can be expected to have high body absorption and high functionality such as skin elasticity. In general, low molecular weight collagen is used in health food, cosmetics and general medicine.
  • Collagen is a component of the human body but is also present in large quantities in meat and fish.
  • pig shells, chicken wings, doggantang, chicken feet are known to be present a lot of people are eating a lot.
  • collagen is ingested through meat products of animal foods, but a small amount of collagen in plant foods is present.
  • Mainly dried persimmons, tofu chungcha, persimmon leaf tea, etc. There is a side that is difficult to expect collagen effect through the intake of such foods.
  • the connective tissue of the dermis which is closely related to skin aging, is mainly composed of collagen and elastin, and collagen is the main protein of connective tissue, accounting for 70-80% of the dry weight of the dermis, giving elasticity and strength to the skin. It plays a large role in maintaining moisturizing. Degradation and content of collagen due to photoaging and natural aging will adversely affect the skin such as wrinkle formation, rough skin, reduced elasticity and dryness. As such, collagen plays an important role in maintaining the elasticity and moisturizing of the skin, which can be attributed to proteins essential for cell composition and production. Therefore, in terms of nutrition, high quality protein intake is closely related to skin health.
  • Eating cosmetics are mainly made up of collagen products extracted from fish, meat and vegetables.
  • Collagen is a type of fibrous protein, which is the main component of connective tissue that forms the skeleton such as muscle, skin, cartilage, cornea, and bone of the human body.
  • the amino acids hydroxyproline, glycine, and serine affect the elasticity and moisturizing of the skin.
  • some pharmaceutical companies and cosmetics manufacturers are importing collagen raw materials from Japan and the United States. Cheong Contract 'Collagen Plus 3000', Hanmi Pharm's New Collagen, Cheil Pharm's Collagen 100, Seed Tree's Collagen 1000, etc. Products.
  • Foreign products such as 'collagen gold' and 'pure collagen SD' are also made from pure collagen. As such, a product with a high content of collagen in the domestic and foreign market was launched. The problem is that there is a lack of research on whether eating collagen can increase the body's collagen content.
  • the collagen crystal 100 of Salom cosmetics is made from collagen extracted from pork skin, and the Jeju horse placenta collagen is literally concentrated in the placenta of horses. Used.
  • collagen products extracted from shark fins, pig placenta or skin and sheep placenta are also included.
  • the ancestors consumed animals or fish, which are known as collagen sources, for a long time or cut them at a suitable temperature to make them gelatinous.
  • collagen When collagen is boiled, it melts into a sticky gelatinous state.
  • Gyoro is made by eating donkey skin shell glue, rust shell rust shell bridge, turtle shell shell ear bridge bridge, Zara shell shell, etc.
  • Donkey shells for making glue, nocturnal bridge, earpan bridge, tortoise bridge, nocturnal shells, turtles and growing shells are not easy to obtain and are expensive, so chicken feet, which can be easily obtained in large quantities, were eaten and eaten like jelly.
  • the skates, or doggantang and the like can be said to be a method of manufacturing a conventional food designed to effectively ingest the collagen component of the animal.
  • Nutrient Enhancement Extract and Jelly Zero Method using Chicken Feet consists of putting chicken feet and soda pastes such as spawning and signing together and boiling them at 100 ⁇ 130 °C high temperature and high pressure for 2 ⁇ 3 days to make products.
  • As an overseas patent there is a product made using imported collagen raw material with molecular weight of 30,000 ⁇ 50,000 manufactured by low temperature and low molecular patent process.
  • Inner beauty products using collagen developed as the above-mentioned conventional technology as raw materials are the academic ordinances of the academic world that have been found so far that collagen cannot be produced in the human body as expected when human ingested, and is the official view of the Food and Drug Administration. .
  • the existing published inventions are used at a level to consume active ingredients by using a strong acid or adding a herbal medicine to help hydrolysis or digestion and boiling together or fermenting. This is a problem that must go through a complex manufacturing process, there is a problem that a large amount of harmful industrial waste is generated using a strong acid during the process of separating collagen from animal tissue.
  • the biggest problem is that the theoretical basis for the absorption and biosynthesis of collagen to eat is scarce.
  • the present inventors are studying a method for producing a collagen low molecular weight peptide without using a strong acid, and utilizing calcium (hydrogen calcium carbonate) obtained from shells in chicken feet, collagen rich in chicken feet as a low molecular peptide
  • the present invention has been completed by developing a method of degrading and developing a product in which the obtained collagen low molecular peptide is mixed with various functional herbal ingredients, for example, a product that satisfies the palatability and nutrition of pets.
  • Another object of the present invention is a product utilizing the collagen low-molecular weight peptide prepared by the above method, for example, a nutritious snack product of the highest quality (more than holistic grade) without preservatives, antibiotics, artificial additives, etc.
  • a nutritious snack product of the highest quality more than holistic grade
  • it provides a collagen supply of low molecular weight peptides to help the hair, fracture and bone health, and herbal medicines to help collagen biosynthesis.
  • the present invention comprises the steps of 1) washing the chicken feet with drinking water and putting them in a water bath to add high pressure steam at 100 to 125 ° C. and 1.5 to 2.5 atm for 10 to 30 minutes; 2) decomposing the sterilized chicken feet with a weak acid containing calcium rather than a strong acid; 3) removing fat from the degraded chicken feet and concentrating; It provides a method for producing a collagen low molecular peptide comprising a; and 4) the fat removed chicken feet fermented for 2 to 3 days by adding a mixed lactic acid strain.
  • the present invention comprises the steps of 1) preparing a collagen low molecular peptide by the above method; And 2) mixing with the medicinal herb comprising the collagen low molecular weight peptide and rat eye bean, black bean, soft meat and nutmeg and concentrating by low-temperature pressure reduction method to prepare a hot water solution. It provides a method for producing.
  • the present invention comprises the steps of 1) preparing a collagen low molecular peptide by the above method; 2) preparing the extracted solution by mixing the collagen low-molecular weight peptide and herbal medicine including hyssop, tofu, safflower seed, Chinese quince, cinnamon and agar; And 3) kneading the lyophilized powder with powder to prepare a formulation selected from the group consisting of pill or tablet powder, jelly (high), and capsule (soft hard). Provide a method.
  • a feature of the present invention is a method for producing a collagen low molecular peptide and a degenerative musculoskeletal system such as an animal nutrition food or bone joint having excellent palatability and efficacy by selecting a natural product that helps to promote the health of a companion animal using the collagen low molecular peptide. It is to provide a method for producing a food that helps to treat or prevent a disease.
  • the present invention comprises the steps of 1) washing the chicken feet with drinking water and putting it in a hot water pot to apply high pressure steam at 100-125 ° C. and 1.5-2.5 atm for 10-30 minutes; 2) decomposing the sterilized chicken feet with a weak acid containing calcium rather than a strong acid; 3) removing fat from the degraded chicken feet and concentrating; It provides a method for producing a collagen low molecular peptide comprising a; and 4) the fat removed chicken feet fermented for 2 to 3 days by adding a mixed lactic acid strain.
  • the hydrogen calcium is preferably obtained from shells or corals, and more preferably calcium hydrogen carbonate.
  • the mixed lactic acid strains preferably include Bacillus Subtillus, Saccaromyces Cerevisiae, Lactobacillus Acidophillus, Lactobacillus Brevis, and Lactobacillus Plantarun.
  • the present invention comprises the steps of preparing a collagen low molecular peptide; And 2) mixing the low-molecular-weight peptides with the herb including black eye beans, black beans, soft muscles, and nutmeg to meet the palatability of the companion animal and conducive to health. It provides a method for producing a companion animal nutrition supplement composition using a collagen low molecular peptide comprising.
  • the second step it is preferable to further include the step of mixing an excipient in the sugar solution, wherein the excipient is rice bran, ganggang And it is more preferable to include soft meat, and moreover, after mixing the excipients, it is more preferable to prepare the composition as a dry powder by a method such as lyophilization, heat drying, microwave device drying and the like.
  • the present invention comprises the steps of 1) preparing a collagen low molecular peptide by the above method; 2) preparing the extracted solution by mixing the collagen low-molecular weight peptide and herbal medicine including hyssop, tofu, safflower seed, Chinese quince, cinnamon and agar; And 3) kneading the lyophilized powder with powder to prepare a formulation selected from the group consisting of pill or tablet powder, jelly (high), and capsule (soft hard). Provide a method.
  • Food prepared using the collagen low molecular weight peptide is a composition that helps degenerative musculoskeletal disorders.
  • the concentrate is kneaded with powdered powder, pill or tablet powder, jelly (coated) and It is desirable to prepare a formulation selected from the group consisting of capsules (soft hard).
  • the step 2) it is preferable to additionally include the step of mixing an excipient in the appetizer, wherein the excipient comprises a gangan fine powder More preferably, after the excipients are mixed, it is more preferable to prepare the composition in a dry powder or jelly form by a method such as lyophilization, thermal drying, or microwave device drying.
  • the present invention is to prepare a low-molecular collagen peptide proved to be effective in chicken feet with a weak acid containing hydrogen calcium rather than a strong acid such as sulfuric acid or hydrochloric acid conventionally used, using the low-molecular collagen peptide, etc. as a raw material for nutrition of pets It can effectively improve the deficiency situation.
  • the composition of the present invention can significantly improve the health of companion animals by using animal protein as a main raw material and the existing nutritional supplement products focusing on conventional vegetable protein.
  • composition of the present invention has the effect of drastically improving degenerative musculoskeletal disorders and dramatically improving degenerative diseases that may help with collagen supply.
  • Collagen 25% of the total protein that makes up the human body is collagen.
  • Collagen the main component of connective tissue such as skin and bone ligament muscles, is a form in which three strands of polymer proteins are tightly bound into a trust structure.
  • Collagen is insoluble in water, dilute acid and dilute alkali, but dissolves in gelatin state when boiled.
  • Collagen has been shown to be effective in osteoporosis, knee arthritis, brain development, skin care and moisturizing effect, enhancement of immunity and growth, arteriosclerosis, Hemostasis, vision recovery and the like are known.
  • the amino acids that make up collagen include proline, oxyproline, [hydroxyproline] glycine, and glutamic acid.
  • Collagen is added to foods made of bone, joint, and skin such as jokbal, beef bone broth, chicken feet, pork shell, and crucible. Contains a lot.
  • Chicken feet are a by-product of slaughter. Chicken feet are composed of bones, joints, cartilage, tendons, and muscles, and are rich in calcium, bone marrow, protein, collagen and other trace elements.
  • Collagen which is found in chicken feet, is the main protein of all connective tissues that make up the human body, including skin, blood vessels, bones, teeth, and muscles. About 16% of the average human body weight is composed of protein, of which about one third is composed of collagen.
  • Collagen is shaped like a long thin rod and supports our body. Collagen is made from chondrocytes, myofibers, osteoblasts, dermal fibroblasts, and the like. Collagen content in the skin is about 70%, the dermis accounts for more than 90%. Keeps your joints healthy and keeps your skin elastic.
  • Collagen is a fibrous protein composed of amino acids, which are mainly present in the membranes, cartilage, teeth, hair, muscles, bones and skin that surround organs and strengthen joints.
  • Chicken foot bones are not thick, cartilage, and chondroitin is present in the cartilage, and glycoproteins such as glycine and connective tissue proteins such as collagen and elastin are contained.
  • Connective tissue has a large amount of extracellular material in the space between tissue cells, and can be divided into native connective tissue, cartilage and bone blood, depending on the type and arrangement of the material.
  • the dermis of the skin of the intrinsic connective tissue is a loose connective tissue in which collagen, a fibrous protein, is interspersed, and the tendon that connects muscles to bone is densely or irregularly packed with collagen. Dense connective tissue in an array.
  • Chicken feet have long been known to be good for degenerative arthritis, and recently, it is said that it is helpful for skin beauty and lowering blood pressure.
  • Induced protein obtained by boiling animal skin with hot water is gelatin.
  • Gelatin cannot be called collagen in a strict sense.
  • Amino acids constituting collagen include proline, oxyproline, glycine, glutamic acid, and the like.
  • Bone quality is also a kind of collagen as a substance that forms the intercellular matrix of bone tissue. Collagen production is lowered in the body from the 20s, less than half in the 40s. Pig's skin, pig's feet, and crucibles contain collagen, especially chicken feet.
  • Collagen has been known so far, such as osteoporosis, knee arthritis, brain development, skin care and moisturizing effect, immunity enhancement and growth development, arteriosclerosis, hemostasis, vision recovery is known.
  • Vitamins b1, b2, b6, c, e and the like, and other trace elements are as follows. Niacin 1.20mg, sodium 30.00mg, zinc 0.1mg, phosphorus 21.0mg, lipid 0.20mg, iron 0.5mg, potassium 65.0mg, calcium 2.0mg, cholesterol 5.40mg, ash 0.20mg.
  • the present invention constituted as described above increases the safety when used in foods, medicines, cosmetics, etc. by using hydrogen calcium rather than strong acid such as sulfuric acid or hydrochloric acid used in the production of conventional collagen low molecular weight peptide, the collagen low molecular weight
  • the nutritional supplement composition prepared by using peptides is a prescription nutritional formula that reflects the habits and constitutions of carnivorous pets while satisfying the preference of pets. Knee and hip dislocations and fractures caused by weakening of dermatitis, fungal skin and musculoskeletal It can be applied to a wide range of diseases, etc., and can also help excellent recovery from viral diseases or diseases.
  • the companion animal nutrition supplement composition of the present invention can be used as a premium prescription nutritional product for health promotion of pets. It is a prescription nutrient that reflects the habits and constitutions of carnivorous pets. It can be widely applied to knee and hip dislocations and fracture diseases caused by weakening of dermatitis, fungal skin, and musculoskeletal. In addition, it can be an excellent help in the recovery of viral diseases and conditions.
  • FIG. 1 is a process flow diagram illustrating a method of preparing a composition for nutritional supplements for pets according to the present invention.
  • FIG. 2 is a graph measuring the content of low molecular weight collagen produced by various fermentations as hydroxyproline (Hydroxyproline) content, which is an indicator component present in collagen.
  • sample A is a general fermentation process application
  • sample B is the case of using conventional rice wine lactic acid bacteria
  • sample C is the case of using the fermentation method of the present invention.
  • Figure 3 is a graph measuring the skin moisture content and elasticity using the collagen low molecular weight peptide contained in the composition for nutritional supplements of the present invention as an anti-aging raw material.
  • Figure 4 is a table showing the components and content of the collagen low molecular weight peptide prepared in the present invention.
  • collagen a polymer protein compound that is not absorbable to the human body, was fermented and developed by the following method to prepare a low molecular peptide.
  • chicken feet were autoclaved. Specifically, chicken feet were purchased from the market and washed with drinking water, and then claws and scales were removed and washed clean with a high-pressure hot water jet. 10Kg of washed chicken feet were placed in a water bath and sterilized with steam for 10-30 minutes by applying pressure of 110-125 ° C and 1.5-2.5 atmospheres.
  • Collagen low molecular weight peptides were prepared from the sterilized chicken feet. Specifically, 10 kg of sterilized chicken feet and drinking water was put in a water bath and boiled, and then boiled by heating over medium heat for 24 to 72 hours without applying pressure at 60 to 99.9 ° C. At this time, the drinking water is preferably about 1.2 to 2 times the chicken feet. More preferably, 1.5 times of drinking water was put in and boiled, but supplemented from time to time.
  • hydrochloric acid or sulfuric acid is used to make collagen into a low-molecular peptide, but in the present invention, when boiling high-molecular collagen, it is boiled with hydrogen calcium, which is mainly used to remove food additives or active oxygen.
  • the hydrogen calcium was used as a commercial product in which a high temperature was applied to shells or corals to deposit hydrogen, and precisely, a large amount of calcium hydrogen carbonate was contained.
  • a product in which hydrogen is bonded in a state in which the bark shell is heated to a high temperature was used.
  • Hydrogen calcium has been used as a food additive to control the acidity, so there is no limit on the capacity, but it is suitable to be around 10g, showing a pH of about 4.5.
  • the heating time may satisfy a total of 24 to 55 hours.
  • Collagen low molecular weight peptides prepared in this manner are not limited in use. It can be used as a raw material for foods, medicines, cosmetics, etc., which use collagen low molecular weight pipide as a raw material or a product, as well as for pet food.
  • Example 1 In preparing the collagen low molecular peptide of Example 1, it was prepared using 10 g of sulfuric acid instead of calcium hydrogen. In addition, fermentation broths such as lactic acid bacteria or jade jade bacteria were added and fermented during the final fermentation.
  • Fresh rice wine with live lactic acid bacteria was purchased and used.
  • Boiled chicken feet were made into gelatin, and then mixed with powders such as hyssop, dagyeok, songjeol and thawed blood to prepare dry powder.
  • Example 2 10 kg of the collagen low molecular weight peptide prepared in Example 1 was added 1 kg of rat eye beans, 1 kg of black beans, 1 kg of soft meat and 0.3 kg of nutmeg, and mixed with 25 L of drinking water to obtain 18 L of hot water, followed by honey After mixing 1 L, 15 L of a highly concentrated liquid composition was prepared by using a low pressure reduction concentrator.
  • the herb is suitable for individual symptoms and can be appropriately modified depending on the palatability of the dog and the efficacy of the herb. It can be used as a liquid composition in this step.
  • the herbal medicine was used for human consumption, Sukjihwang, earl, cheonggung, donkey, reed, dandelion, etc., but these herbs are not suitable for the pet's preference. Therefore, the present inventors used such a component instead of these.
  • rats and black beans were used in place of the nutrient-rich roots of vegetable nutrient hormones, and also has a high protein content (particularly, plant female hormone content).
  • excipient consisting of rice bran, ganggang, and soft muscle was added to the highly concentrated liquid composition prepared above.
  • the ratio of the amount of excipient is suitably about 100-200 parts by weight of the excipient 100 parts by weight of the liquid composition.
  • Many excipients are less effective and less difficult to formulate. If you use rice bran, ganggang, soft meat as an excipient, there is no need to artificially add a separate binder.
  • the liquid composition mixed with the excipient was a freeze-drying method or hot air drying to prepare a composition of dry granules.
  • the freeze-drying conditions of the freeze dryer should satisfy the conditions of general foods, and during the hot air drying, it may be dried for about 5-10 hours at around 70 ° C ( ⁇ 5 ° C).
  • composition suitable for use in general dietary fiber and raw materials having high physiological activity were developed, and granules, pills, powders, and the like were prepared based on the specialized formulation technology.
  • the preparation of the collagen low molecular weight peptide is the same as in Example 2. However, the same process is used except that the content of the collagen low-molecular peptide and herbal medicine is mixed by adding 2 kg of rat bean, 2 kg of black bean, 2 kg of soft bean meat and 0.6 kg of nutmeg.
  • the preparation of the collagen low molecular weight peptide is the same as in Example 1. However, the same process is used except that the content of the collagen low molecular weight peptide and herbal medicine is mixed with 0.5 kg of black bean, 0.5 kg of black beans, 0.5 kg of soft bean meat and 0.15 kg of nutmeg.
  • the herb is suitable for individual symptoms and can be appropriately modified depending on the palatability of the dog and the efficacy of the herb. It can be used as a liquid composition in this step.
  • the herbal medicine was used for human consumption, Sukjihwang, earl, cheonggung, donkey, reed, dandelion, etc., but these herbs are not suitable for the pet's preference. Therefore, the present inventors used such a component instead of these.
  • rats and black beans were used in place of the nutrient-rich roots of vegetable nutrient hormones, and also has a high protein content (particularly, plant female hormone content).
  • excipient consisting of rice bran, ganggang, and soft muscle was added to the highly concentrated liquid composition prepared above.
  • the ratio of the amount of excipient is suitably about 100-200 parts by weight of the excipient 100 parts by weight of the liquid composition.
  • Many excipients are less effective and less difficult to formulate. If you use rice bran, ganggang, soft meat as an excipient, there is no need to artificially add a separate binder.
  • the liquid composition mixed with the excipient was a freeze-drying method or hot air drying to prepare a composition of dry granules.
  • the freeze-drying conditions of the freeze dryer should satisfy the conditions of general foods, and during the hot air drying, it may be dried for about 5-10 hours at around 70 ° C ( ⁇ 5 ° C).
  • composition suitable for use in general dietary fiber and raw materials having high physiological activity were developed, and granules, pills, powders, and the like were prepared based on the specialized formulation technology.
  • 25 kg of drinking water was added to 10 kg of the collagen low molecular weight peptide prepared in Example 1 by adding 1 kg of hyssop, 1 kg of worms, 1 kg of quince, 1 kg of safflower seed, 1 kg of ginseng, 1 kg of ogapi and 1 kg of dandelion, and 1 kg of vigor.
  • 8 L of a highly concentrated liquid composition was prepared by using a low temperature vacuum concentrator.
  • the filtrate was prepared in a pouch to prepare a food having a function of helping the degenerative musculoskeletal inflammation of the liquid.
  • Esoteric, tofu, quince, safflower seed, gyeji, agapi and the like used in the above examples are medicinal herbs to help musculoskeletal disorders
  • ⁇ is a treasure trove of calcium and contains a lot of natural female hormone components that help collagen biosynthesis in the body It is widely known as a food that helps bone health.
  • Magnesium is not only contains a large amount of dietary fiber required by modern people, but also has high viscosity, so it is excellent in formulating it into pills, acids, tablets, etc. without using a separate binder.
  • the content of the low molecular weight collagen produced in the fermentation of Example 1 and Comparative Examples 1, 2 and 3 of the present invention was measured by the content of hydroxyproline (Hydroxyproline) which is an indicator component present in collagen.
  • the measurement of collagen peptides at the low molecular level was verified by checking the hydroxyproline content, which is a component of collagen.
  • Sample A is a general fermentation process (Comparative Example 1)
  • Sample B is a case using the conventional rice wine lactic acid bacteria (Comparative Example 2)
  • Sample C is a case using the fermentation method of Example 1 of the present invention.
  • the results of FIG. 2 show that Sample A has an average value of 0.539111, Standard Deviation 0.008328, Sample B has an average value of 0.383147, Standard Deviation 0.006864, Example 1 (Sample C) has an average value of 0.692833, Standard Deviation of 0.010135, and Step Mountain has an average value of 0.303793.
  • a deviation of 0.00595 was measured.
  • Hydroxyproline is the only amino acid constituting collagen, and the hydroxyproline content can be used to determine the presence of collagen.
  • Samples A, B, and C were prepared using the same materials and preparation conditions to determine the hydroxyproline content.
  • Sample C was confirmed to have a high hydroxyproline content. It was confirmed that the hydroxyproline content of about 2 times higher than cinnamic acid product using chicken feet as a raw material was very excellent in the preparation of collagen low molecular weight peptide.
  • Example 1 of the present invention As a result of using the collagen low molecular weight peptide prepared in Example 1 of the present invention as a raw material of an anti-aging product, it was confirmed that it showed excellent efficacy in promoting skin health (FIG. 3).
  • the moisture and elasticity of the skin are related to the dermal layer.
  • the dermal layer contains collagen fibers, elastin fibers, and substrates, which are highly related to the moisturizing and elasticity of the skin. Decreased skin activity or low collagen protein content due to nutritional imbalance, UV exposure and aging.
  • the components of the dermis are closely related to protein nutrients, which play an important role in body composition. In particular, the increase of protein in the body is a factor that can affect the skin elasticity and pore change, the change of intracellular moisture is a factor that affects the skin moisture state.
  • the collagen low molecular weight peptide prepared by the advanced bio method of the present invention had a high protein content and a high rate of absorption in the body after ingestion.
  • Collagen low-molecular inner beauty foods are highly effective in promoting collagen biosynthesis in middle-aged and post-menopausal women by using herbs containing high amounts of plant female hormones that help biosynthesis.
  • the composition of the present invention can contribute to improving skin nutrition of pets and health of hair.
  • a Chinese medicine made from the same collagen low molecular weight peptide as a raw material for musculoskeletal disorders has been shown to be effective in treating knee arthritis (paper; clinical study on 30 cases of knee arthritis patients taking KJ 27, Korea Korean Journal of Oriental Physiology, 201, 2, pp. 69-75). Therefore, it is possible to prevent various diseases as well as to help treat diseases caused by aging of pets and breeding in restricted spaces and the weakening of organs constituting the fracture or musculoskeletal system.
  • composition prepared in Examples 2 to 4 was to be ingested in the test animal.
  • test animal was selected as a Maltese species with high preference among companion animals, and the health status was classified according to the FCI standard and examined by the veterinarian.
  • the palatability test selects three or four farms or centers with several cats with a relatively difficult diet and divides them into dogs, adult dogs, and old dogs, and then classifies health as standard and weak. Three kinds of samples prepared in the present invention were made and administered 2-3 times to examine the preference.
  • Evaluation procedure Fill out the questionnaire and explain the tasting instructions to the owner.
  • Tasting procedure Prepare three prototypes and feed them to dogs to observe their preferences.
  • Sample A is a raw material and sample B is a mixture of soft gynecology and Baibok-ryeong for palatability evaluation.
  • Sample C is a mixture of fragrance flat stomach granules. Sample A liked more than 80%, sample B obtained 70%, and sample C obtained about 65%.
  • the program used for statistical analysis is IBM SPSS Statistics 20.
  • the patient's condition was recorded and analyzed by determining the gender, age, body type, housing type, Shenzhen, family history, causes of knee osteoarthritis, treatment status, exercise volume, and occupation. Since knee osteoarthritis occurs more frequently in women than in men, we wanted to see the distribution according to gender differences.
  • the side effects and palatability of the food composition (hereinafter referred to as KJ27) prepared in Example 5 were investigated, and the cure rate according to the arthritis treatment period was considered.
  • the pharmaceutical composition of the food composition is prepared by adding collagen, low molecular weight peptide, ogapi, hyssop, Haedongpi, Sukjihwang, Angelica, Cheongung, Baekjak, etc., but only slightly different from the medicinal herbs of the present invention to prepare extracts for degenerative joint diseases. It was prepared substantially similarly to the preparation of the food composition of Example 5.
  • the customer satisfaction subjective evaluation (question) result of the food composition is shown in Table 1 below.

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Abstract

La présente invention concerne un peptide de collagène de faible poids moléculaire et un procédé de préparation d'une composition alimentaire à l'aide de celui-ci et, plus spécifiquement, un procédé de préparation d'un peptide de collagène de faible poids moléculaire, le procédé consistant à stériliser des pieds de poulet sous vapeur haute pression, à les dégrader avec un acide faible contenant de l'hydrure de calcium, à éliminer la matière grasse des pieds de poulet dégradés et à les concentrer, puis à y mettre des souches bactériennes d'acide lactique mélangées de façon à provoquer une fermentation, ainsi qu'un procédé de préparation d'une composition de supplément nutritionnel pour animal de compagnie, le procédé consistant à préparer un liquide de décoction par la préparation du peptide de collagène de faible poids moléculaire préparé par le procédé associé, à mélanger, avec le peptide, des herbes médicinales comprenant Rhynchosia Nolubilis, du soja noir, Nelumbo nucifera des graines de Gaertner et des graines de Myristica fragrans Houttuyn, et à les concentrer au moyen d'un procédé de décompression à basse température; et une composition alimentaire, le procédé de préparation d'un liquide de décoction extrait par mélange, avec le peptide, d'herbes médicinales comprenant Acyranthes bidentés Blume, Eucommia ulmoide, des graines de Carthami Flos, des coings chinois, Cinnamomi Ramulus et acanthopanax. La présente invention augmente la sécurité lorsqu'elle est utilisée dans des aliments, des médicaments, des cosmétiques et similaires, en utilisant de l'hydrure de calcium, qui n'est pas un acide fort comme l'acide sulfurique ou l'acide chlorhydrique, qui est utilisé dans une préparation classique d'un peptide de collagène de faible poids moléculaire. La composition de supplément nutritionnel pour animal de compagnie préparée à l'aide du peptide de collagène de faible poids moléculaire préparé est un supplément nutritionnel prescrit, qui satisfait la préférence d'un animal de compagnie et reflète les habitudes et les goûts d'un animal de compagnie carnivore, ce qui est largement applicable à la dermatite, aux peaux fongiques, à la luxation du genou et de la hanche provoquée par une faiblesse musculosquelettique, les maladies des fractures et similaires.
PCT/KR2017/005232 2016-05-20 2017-05-19 Peptide de collagène de faible poids moléculaire et procédé de préparation d'une composition alimentaire à l'aide de celui-ci Ceased WO2017200340A2 (fr)

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