WO2017201331A3 - Oligonucleotide sequences for detection of low abundance target sequences and kits thereof - Google Patents

Oligonucleotide sequences for detection of low abundance target sequences and kits thereof Download PDF

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Publication number
WO2017201331A3
WO2017201331A3 PCT/US2017/033401 US2017033401W WO2017201331A3 WO 2017201331 A3 WO2017201331 A3 WO 2017201331A3 US 2017033401 W US2017033401 W US 2017033401W WO 2017201331 A3 WO2017201331 A3 WO 2017201331A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
sequences
kits
low abundance
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2017/033401
Other languages
French (fr)
Other versions
WO2017201331A2 (en
Inventor
Vlada MELNIKOVA
Mark Erlander
Peter CROUCHER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cardiff Oncology Inc
Original Assignee
Trovagene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trovagene Inc filed Critical Trovagene Inc
Publication of WO2017201331A2 publication Critical patent/WO2017201331A2/en
Publication of WO2017201331A3 publication Critical patent/WO2017201331A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure relates to the detection of target nucleic acid sequences present in low-abundance relative to corresponding non-target or wild-type nucleic acid sequence in a sample. In particular, compositions comprising oligonucleotide sequences suitable for use in enrichment and amplification methods allow for a substantially greater level of detection sensitivity of mutant or target sequence within a high background of wild type sequence.
PCT/US2017/033401 2016-05-20 2017-05-18 Oligonucleotide sequences for detection of low abundance target sequences and kits thereof Ceased WO2017201331A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662339537P 2016-05-20 2016-05-20
US62/339,537 2016-05-20
US201662370924P 2016-08-04 2016-08-04
US62/370,924 2016-08-04

Publications (2)

Publication Number Publication Date
WO2017201331A2 WO2017201331A2 (en) 2017-11-23
WO2017201331A3 true WO2017201331A3 (en) 2017-12-21

Family

ID=60326213

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/033401 Ceased WO2017201331A2 (en) 2016-05-20 2017-05-18 Oligonucleotide sequences for detection of low abundance target sequences and kits thereof

Country Status (1)

Country Link
WO (1) WO2017201331A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3814500A4 (en) * 2018-06-27 2022-03-16 The Trustees of Indiana University PROCEDURE FOR ANALYZING DNA IN URINE
CN116064753B (en) * 2018-08-28 2026-04-14 深圳联合医学科技有限公司 High-efficiency sequencing library construction method, primer set and kit
US20250340934A1 (en) * 2024-05-06 2025-11-06 Gt Molecular, Inc. Selective blocking to detect and amplify low abundant template

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130005585A1 (en) * 2011-05-20 2013-01-03 Fluidigm Corporation Nucleic acid encoding reactions

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130005585A1 (en) * 2011-05-20 2013-01-03 Fluidigm Corporation Nucleic acid encoding reactions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ASCIERTO, PA ET AL.: "The Role of BRAF V600 Mutation in Melanoma", JOURNAL OF TRANSLATIONAL MEDICINE, vol. 10, no. 85, 9 July 2012 (2012-07-09), pages 1 - 9, XP021126777 *
YO, J ET AL.: "Detection of BRAF Mutations from Solid Tumors using TumorplexTM Technology", METHODSX, vol. 2, 12 June 2015 (2015-06-12), pages 316 - 322, XP055450361 *

Also Published As

Publication number Publication date
WO2017201331A2 (en) 2017-11-23

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