WO2018002955A1 - Formulation de bio-inoculants destinée à l'agriculture à durée de conservation améliorée - Google Patents

Formulation de bio-inoculants destinée à l'agriculture à durée de conservation améliorée Download PDF

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Publication number
WO2018002955A1
WO2018002955A1 PCT/IN2017/050263 IN2017050263W WO2018002955A1 WO 2018002955 A1 WO2018002955 A1 WO 2018002955A1 IN 2017050263 W IN2017050263 W IN 2017050263W WO 2018002955 A1 WO2018002955 A1 WO 2018002955A1
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WO
WIPO (PCT)
Prior art keywords
micro
slurry
range
organism
emulsion
Prior art date
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Ceased
Application number
PCT/IN2017/050263
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English (en)
Inventor
Preethi MARIMUTHU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tropical Biosciences Pvt Ltd
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Tropical Biosciences Pvt Ltd
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Filing date
Publication date
Application filed by Tropical Biosciences Pvt Ltd filed Critical Tropical Biosciences Pvt Ltd
Publication of WO2018002955A1 publication Critical patent/WO2018002955A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom

Definitions

  • Figure 3(a) depicts the plant growth promoting effect observed in soyabean plant with the use of beads comprising encapsulated micro-organisms (B) vs when no treatment with encapsulated micro-organisms is provided to soyabean seeds (A), in accordance with an embodiment of present disclosure.
  • Figure 3(b) depicts the plant growth promoting effect observed in groundnut plant with the use of beads comprising encapsulated micro-organisms (B) vs when no treatment with encapsulated micro-organisms is provided to groundnut seeds(A), in accordance with an embodiment of present disclosure.
  • a method of encapsulation of at least one micro-organism as described herein wherein said method further comprises of drying said hardened microbeads to obtain beads.
  • a method of encapsulation of at least one micro-organism comprises the steps of: (a) preparing a slurry comprising 10 gm casein, 15gm lactose in 100ml water by heating the ingredients at a temperature in the range of 85 °C; (b) cooling the slurry to room temperature (25-35°C), and adjusting the pH with gluconic acid of said slurry in the range of 5.5-6.5 to obtain slurry II; (c) obtaining Arbuscular Mycorrhizal spores; (d) washing said spores with a buffer such that the pH of micro-organism is in the range of 5.6-6.5; (e) dispersing said micro-organisms in said slurry II and stirring at 500- 1500rpm to obtain a mixture; (f) adding 200ml of vegetable oil to said mixture to obtain an emulsion; (g) stirring said emulsion at a speed
  • a method of encapsulation of at least one micro-organism comprises the steps of: (a) preparing a slurry comprising 2 gm casein, 20gm sucrose in 100ml water by heating the ingredients at a temperature of 85°C; (b) cooling the slurry to room temperature (25-35°C), and adjusting the pH with gluconic acid of said slurry in the range of 5.5- 6.5 to obtain slurry II; (c) obtaining Arbuscular Mycorrhizal spores; (d) washing said spores with a buffer such that the pH of micro-organism is in the range of 5.6-6.5; (e) dispersing said micro-organisms in said slurry II and stirring at 500-1500rpm to obtain a mixture; (f) adding 200ml of vegetable oil to said mixture to obtain an emulsion; (g) stirring said emulsion at a speed in the range of 200
  • a method of encapsulation of at least one micro-organism comprises the steps of: (a) preparing a slurry comprising 3 gm casein, lOgm lactose in 100ml water by heating the ingredients at a temperature of 85°C; (b) cooling the slurry to room temperature (25- 35°C), and adjusting the pH with gluconic acid of said slurry in the range of 5.5-6.5 to obtain slurry II; (c) obtaining Arbuscular Mycorrhizal spores; (d) washing said spores with a buffer such that the pH of micro-organism is in the range of 5.6-6.5; (e) dispersing said micro-organisms in said slurry II and stirring at 500-1500rpm to obtain a mixture; (f) adding 200ml of vegetable oil to said mixture to obtain an emulsion; (g) stirring said emulsion at a speed in the
  • an encapsulated micro-organism(s) obtained by a method as described herein, wherein said molarity of inorganic salts is 0.1-0.3M.
  • an encapsulated micro-organism(s) obtained by a method comprises the steps of: (a) preparing a slurry comprising 2 gm casein, 20gm sucrose in 100ml water by heating the ingredients at a temperature in the range of 85°C; (b) cooling the slurry to room temperature (25-35°C), and adjusting the pH with gluconic acid of said slurry in the range of 5.5-6.5 to obtain slurry II; (c) obtaining Arbuscular Mycorrhizal spores; (d) washing said spores with a buffer such that the pH of micro-organism is in the range of 5.6-6.5; (e) dispersing said micro-organisms in said slurry II and stirring at 500- 1500rpm to obtain a mixture; (f) adding 200ml of vegetable oil to said mixture to obtain an emulsion; (g) stirring said emulsion at a speed in the
  • an encapsulated micro-organism(s) obtained by a method comprises the steps of: (a) preparing a slurry comprising 2 gm casein, lOgm lactose in 100ml water by heating the ingredients at a temperature in the range of 70 °C; (b) cooling the slurry to room temperature (25-35°C), and adjusting the pH of said slurry in the range of 5.5-6.5 to obtain slurry II; (c) obtaining Arbuscular Mycorrhizal spores; (d) washing said spores with a buffer such that the pH of micro-organism is in the range of 5.6-6.5; (e) dispersing said micro-organisms in said slurry II and stirring at 500-1500rpm to obtain a mixture; (f) adding 100ml of seasame oil, and 100ml groundnut oil to said mixture to obtain an emulsion; (g) stirring said
  • pH of the microbial suspension upon wash with the buffer should be between 5.5-7 (d) microbial suspension having a pH in the range of 5.5-6.5 was added to the slurry to obtain a mixture (e) mixture and oil having a v/v ratio in the range of 1:2-1:3 are mixed to obtain an emulsion and stirred, the continuous slow stirring causes the separation of oil beads from oil phase.
  • Protein source Sodium caesinate 2% in water w/v 0.66% in emulsion w/v Sugar lactose 15% in water w/v 5% in emulsion w/v
  • sugars which can be used is selected from the group consisting of but not limited to lactose, sucrose, trehalose, starch, dextrin, cellulose.
  • Proteins compatible for use are casein, sodium caesinate, and whey protein isolate.
  • Coating agent that can be used are xanthan gum, gellam gum, arabic gum.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne des micro-organismes micro-encapsulés, et des procédés de production des micro-organismes micro-encapsulés. Les micro-organismes micro-encapsulés peuvent être enduits sur la surface de semences, améliorant ainsi le potentiel de croissance de la plante, puisque les micro-organismes colonisent les parties végétales. Les organismes vivants sont encapsulés dans un polymère perméable, qui permet la perméation des contenus cellulaires et nutritionnels au niveau du noyau et permet la libération contrôlée (10) des endophytes, et la micro-encapsulation protège les micro-organismes des effets toxiques des pesticides/fongicides. Les semences enduites d'endophytes micro-encapsulés agissent comme véhicule d'administration efficace d'application ciblée de microbes.
PCT/IN2017/050263 2016-06-30 2017-06-26 Formulation de bio-inoculants destinée à l'agriculture à durée de conservation améliorée Ceased WO2018002955A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN201641022620 2016-06-30
IN201641022620 2016-06-30

Publications (1)

Publication Number Publication Date
WO2018002955A1 true WO2018002955A1 (fr) 2018-01-04

Family

ID=59388125

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2017/050263 Ceased WO2018002955A1 (fr) 2016-06-30 2017-06-26 Formulation de bio-inoculants destinée à l'agriculture à durée de conservation améliorée

Country Status (1)

Country Link
WO (1) WO2018002955A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110527628A (zh) * 2019-07-30 2019-12-03 南京农业大学 一种啶虫脒降解菌的保护剂及其制备方法和应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3111407A (en) 1960-02-26 1963-11-19 Ibm Methods for making record materials
US3726804A (en) 1969-09-02 1973-04-10 Fuji Photo Film Co Ltd Process for the production of microcapsules containing an oily liquid
US4755468A (en) * 1981-12-29 1988-07-05 Rhone-Poulenc S.A. Inocula of low water activity with improved resistance to temperature and rehydration, and preparation thereof
US4956129A (en) 1984-03-30 1990-09-11 Ici Americas Inc. Microencapsulation process
US20120266332A1 (en) * 2009-09-03 2012-10-18 Advanced Biological Marketing, Inc. Herbicide-resistant inoculant strains

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3111407A (en) 1960-02-26 1963-11-19 Ibm Methods for making record materials
US3726804A (en) 1969-09-02 1973-04-10 Fuji Photo Film Co Ltd Process for the production of microcapsules containing an oily liquid
US4755468A (en) * 1981-12-29 1988-07-05 Rhone-Poulenc S.A. Inocula of low water activity with improved resistance to temperature and rehydration, and preparation thereof
US4956129A (en) 1984-03-30 1990-09-11 Ici Americas Inc. Microencapsulation process
US20120266332A1 (en) * 2009-09-03 2012-10-18 Advanced Biological Marketing, Inc. Herbicide-resistant inoculant strains

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
P SIVA KUMAR ET AL., INT.J.CURR.MICROBIOL.APP.SCI, vol. 3, no. 6, 2014, pages 415 - 422
ROJAN P. JOHN ET AL: "Bio-encapsulation of microbial cells for targeted agricultural delivery", CRC CRITICAL REVIEWS IN BIOTECHNOLOGY, vol. 31, no. 3, 29 September 2010 (2010-09-29), US, pages 211 - 226, XP055407352, ISSN: 0738-8551, DOI: 10.3109/07388551.2010.513327 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110527628A (zh) * 2019-07-30 2019-12-03 南京农业大学 一种啶虫脒降解菌的保护剂及其制备方法和应用

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