WO2018088856A3 - 마이크로유체칩, 삼차원 채널 구조물, 이를 이용한 세포 배양 방법 및 이를 이용한 생리활성 물질의 활성평가 방법 - Google Patents

마이크로유체칩, 삼차원 채널 구조물, 이를 이용한 세포 배양 방법 및 이를 이용한 생리활성 물질의 활성평가 방법 Download PDF

Info

Publication number
WO2018088856A3
WO2018088856A3 PCT/KR2017/012763 KR2017012763W WO2018088856A3 WO 2018088856 A3 WO2018088856 A3 WO 2018088856A3 KR 2017012763 W KR2017012763 W KR 2017012763W WO 2018088856 A3 WO2018088856 A3 WO 2018088856A3
Authority
WO
WIPO (PCT)
Prior art keywords
same
microfluidic chip
cell culture
channel structure
plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2017/012763
Other languages
English (en)
French (fr)
Other versions
WO2018088856A2 (ko
Inventor
정기석
황창모
장세진
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asan Foundation
University of Ulsan Foundation for Industry Cooperation
Original Assignee
Asan Foundation
University of Ulsan Foundation for Industry Cooperation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asan Foundation, University of Ulsan Foundation for Industry Cooperation filed Critical Asan Foundation
Priority to EP17870128.0A priority Critical patent/EP3540042A4/en
Priority to US16/349,217 priority patent/US11692160B2/en
Publication of WO2018088856A2 publication Critical patent/WO2018088856A2/ko
Publication of WO2018088856A3 publication Critical patent/WO2018088856A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C12M1/32—Inoculator or sampler multiple field or continuous type
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/06—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22—Settling tanks; Sedimentation by gravity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Oncology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Fluid Mechanics (AREA)
  • Water Supply & Treatment (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

본 발명의 일 실시예에 따른 마이크로유체칩은 플레이트, 플레이트의 일면에 음각으로 형성되는 브릿지 채널, 브릿지 채널의 일측 단부와 연통하도록 플레이트를 관통하여 형성되는 유입부, 브릿지 채널의 타측 단부와 연통하도록 플레이트를 관통하여 형성되는 배출부 및 브릿지 채널로부터 플레이트의 외측 방향으로 연장되어 공간을 제공하는 적어도 하나의 웰을 포함하고, 브릿지 채널은 곡선, 꺽인선, 호형, 원형, 나선형 또는 다각형으로 형성될 수 있다.
PCT/KR2017/012763 2016-11-10 2017-11-10 마이크로유체칩, 삼차원 채널 구조물, 이를 이용한 세포 배양 방법 및 이를 이용한 생리활성 물질의 활성평가 방법 Ceased WO2018088856A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP17870128.0A EP3540042A4 (en) 2016-11-10 2017-11-10 MICROFLUIDIC CHIP, THREE-DIMENSIONAL CHANNEL STRUCTURE, CELL CULTURE METHOD USING THE SAME, AND ACTIVITY ASSESSMENT METHOD OF A BIOACTIVE SUBSTANCE USING THE SAME
US16/349,217 US11692160B2 (en) 2016-11-10 2017-11-10 Microfluidic chip, three-dimensional channel structure, cell culture method using same, and activity evaluation method of bioactive substance using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2016-0149798 2016-11-10
KR20160149798 2016-11-10

Publications (2)

Publication Number Publication Date
WO2018088856A2 WO2018088856A2 (ko) 2018-05-17
WO2018088856A3 true WO2018088856A3 (ko) 2018-08-09

Family

ID=62109978

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2017/012763 Ceased WO2018088856A2 (ko) 2016-11-10 2017-11-10 마이크로유체칩, 삼차원 채널 구조물, 이를 이용한 세포 배양 방법 및 이를 이용한 생리활성 물질의 활성평가 방법

Country Status (4)

Country Link
US (1) US11692160B2 (ko)
EP (1) EP3540042A4 (ko)
KR (4) KR102007376B1 (ko)
WO (1) WO2018088856A2 (ko)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020005150A1 (en) 2018-06-29 2020-01-02 Johan Kreuger Biofilm culturing
CN112023990B (zh) * 2019-06-03 2023-06-23 利多(香港)有限公司 一种微流控检测芯片及制造方法
CN110283724B (zh) * 2019-07-09 2022-07-29 汇芯(北京)科技有限公司 一种高通量类器官表型筛查系统和方法
CN111774108B (zh) * 2020-06-19 2021-11-12 东南大学 一种壁面分离式螺旋微流控芯片
KR102578640B1 (ko) * 2020-08-14 2023-09-15 주식회사 넥스트앤바이오 미세유체 칩을 활용한 오가노이드의 제조 방법
KR102475225B1 (ko) 2020-08-27 2022-12-07 성균관대학교산학협력단 R2r 공정을 이용한 오가노이드 실시간 모니터링 장치 제조
KR102438496B1 (ko) 2020-09-07 2022-09-02 포항공과대학교 산학협력단 생체외 인공장기 모델 및 세포 치료제로의 적용이 가능한 관류형 이중 근위세뇨관 관상 세포 구조체 및 그 생성 방법
WO2022112968A1 (en) 2020-11-25 2022-06-02 Association For The Advancement Of Tissue Engineering And Cell Based Technologies & Therapies (A4Tec) - Associação Bioreactor for tissue engineering of multi-tissue structure and manufacturing method thereof
CA3197769A1 (en) * 2020-12-04 2022-06-09 Dongeun Huh Engineering of organoid culture for enhanced organogenesis in a dish
TWI793671B (zh) * 2021-07-09 2023-02-21 中國醫藥大學 細胞治療用生物晶片及其製造方法
KR102602884B1 (ko) * 2021-07-15 2023-11-15 중앙대학교 산학협력단 이중층 구조의 세포 배양 칩 및 이를 이용한 세포 배양장치
KR102678639B1 (ko) * 2021-09-06 2024-06-27 서울대학교산학협력단 스페로이드 또는 오가노이드 미세 유체 칩 및 이를 이용한 스페로이드 또는 오가노이드 체외 모델 형성 방법
CN114950581B (zh) * 2022-04-06 2024-02-13 东南大学 一种多轨道螺旋微流控芯片及其制造方法
KR102942137B1 (ko) * 2022-08-08 2026-03-20 주식회사 한국동물대체실험 약물의 지속적인 항암 활성 및 약물의 나노입자제형의 epr 효과를 확인할 수 있는 미세유체 장치
CN115322904B (zh) * 2022-10-12 2023-01-31 成都诺医德医学检验实验室有限公司 一种模拟膀胱结构的类器官培养装置及其应用
KR102771050B1 (ko) 2023-01-04 2025-02-24 한국공학대학교산학협력단 인공 조직의 제조방법
KR102866760B1 (ko) * 2024-01-09 2025-10-01 서울대학교산학협력단 다수의 채널을 포함하는 세포 배양용 미세유체칩
KR102866068B1 (ko) 2024-12-17 2025-09-29 농업회사법인 주식회사 팜우보에 이동식 카메라를 활용한 농작물 모니터링 기반 스마트 수경 재배 시스템

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020106786A1 (en) * 2000-05-15 2002-08-08 Carvalho Bruce L. Microfluidics devices and methods for performing cell based assays
KR20070033685A (ko) * 2005-09-22 2007-03-27 한국과학기술원 미세유체 기술을 이용한 3차원 세포배양 시스템
KR20130011101A (ko) * 2011-07-20 2013-01-30 울산대학교 산학협력단 세포 배양용 마이크로 유동성 플랫폼 및 이를 이용한 세포 배양방법
US20150284668A1 (en) * 2014-04-08 2015-10-08 National Tsing Hua University Cyclic Microfluidic Chip and Method of Using the Same
KR20160018200A (ko) * 2014-08-08 2016-02-17 삼성전자주식회사 미세유동장치

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2575293B1 (fr) * 1984-12-21 1987-03-20 Inovelf Sa Rotor a pipetage dynamique pour dispositif d'analyse a centrifugation
AU4047493A (en) * 1992-04-02 1993-11-08 Abaxis, Inc. Analytical rotor with dye mixing chamber
US5942443A (en) * 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US8276760B2 (en) * 2006-11-30 2012-10-02 Palo Alto Research Center Incorporated Serpentine structures for continuous flow particle separations
WO2009146911A2 (en) * 2008-06-04 2009-12-10 Uwe Marx Organ-on-a-chip-device
KR100997144B1 (ko) * 2008-09-23 2010-11-30 삼성전자주식회사 미세유동장치
LT2970890T (lt) 2013-03-14 2020-07-10 The Brigham And Women`S Hospital, Inc. Kompozicijos ir būdai, skirti epitelinių kamieninių ląstelių padauginimui ir kultivavimui
KR101621211B1 (ko) * 2014-07-15 2016-05-16 고려대학교 산학협력단 항암제 검증용 마이크로 유체칩 및 이의 제조방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020106786A1 (en) * 2000-05-15 2002-08-08 Carvalho Bruce L. Microfluidics devices and methods for performing cell based assays
KR20070033685A (ko) * 2005-09-22 2007-03-27 한국과학기술원 미세유체 기술을 이용한 3차원 세포배양 시스템
KR20130011101A (ko) * 2011-07-20 2013-01-30 울산대학교 산학협력단 세포 배양용 마이크로 유동성 플랫폼 및 이를 이용한 세포 배양방법
US20150284668A1 (en) * 2014-04-08 2015-10-08 National Tsing Hua University Cyclic Microfluidic Chip and Method of Using the Same
KR20160018200A (ko) * 2014-08-08 2016-02-17 삼성전자주식회사 미세유동장치

Also Published As

Publication number Publication date
US20190284517A1 (en) 2019-09-19
KR20180052548A (ko) 2018-05-18
EP3540042A2 (en) 2019-09-18
KR102026329B1 (ko) 2019-09-27
KR20180052547A (ko) 2018-05-18
WO2018088856A2 (ko) 2018-05-17
KR20180052546A (ko) 2018-05-18
EP3540042A4 (en) 2020-07-15
US11692160B2 (en) 2023-07-04
KR102007376B1 (ko) 2019-08-05
KR20180052549A (ko) 2018-05-18
KR102026313B1 (ko) 2019-09-30
KR102026326B1 (ko) 2019-09-27

Similar Documents

Publication Publication Date Title
WO2018088856A3 (ko) 마이크로유체칩, 삼차원 채널 구조물, 이를 이용한 세포 배양 방법 및 이를 이용한 생리활성 물질의 활성평가 방법
USD756808S1 (en) Radar enhanced parking meter
USD756807S1 (en) Radar enhanced parking meter
USD877806S1 (en) Typefaces
WO2016015058A3 (en) Methods of determining tissues and/or cell types giving rise to cell-free dna, and methods of identifying a disease or disorder using same
USD780290S1 (en) Spray lance
WO2016161169A3 (en) Cell culture incubators with integrated cell manipulation systems
WO2017184786A8 (en) Cpf1 complexes with reduced indel activity
CL2016001576S1 (es) Instrumento para secuenciar formado por un cuerpo prismático de cara frontal inclinada, la mitad superior tiene dos porciones de cara superior inclinada, la porción izquierda sobresale hacia adelante y la mitad inferior de la cara frontal inclinada posee una porción resaltada de frente rectangular.
WO2018109170A3 (en) Il-11ra antibodies
GB2556269A (en) Fibrosis model on a chip
USD795465S1 (en) Utility pole
WO2018094356A3 (en) Compositions and methods for target nucleic acid modification
WO2014201015A8 (en) Methods and compositions for target dna modification
CL2009000486S1 (es) Plancha para el cabello, constituida por dos porciones prismaticas rectangulares de vertices redondeados alargadas longitudinalmente, unidas por un extremo mediante un eje y separadas en el extremo opuesto, con paredes frontales convexas y en el lateral derecho de la porcion inferior tres elementos diferentes.
GB2548941A (en) Microfluidic chip with conic bead trapping cavities and fabrication thereof
WO2016073905A8 (en) Microneedle arrays for cancer therapy applications
WO2017087916A3 (en) Thermostable glucose biosensors and uses thereof
WO2015058132A3 (en) Anti-rsp02 and/or anti-rsp03 antibodies and their uses
WO2018048181A3 (ko) 세포 배양 용기
EP4656736A3 (en) Determining cell type origin of circulating cell-free dna with molecular counting
WO2017173354A3 (en) METABOLICALLY STABILIZED DOUBLE STRANDED mRNA
USD754781S1 (en) Sunglasses
WO2018122761A3 (en) Hybrid structures and methods of making the same
USD778022S1 (en) Aerial lift

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17870128

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2017870128

Country of ref document: EP

Effective date: 20190611

WWW Wipo information: withdrawn in national office

Ref document number: 2017870128

Country of ref document: EP