WO2019015937A1 - Procédé et appareil de commande destiné à détecter des bulles dans une chambre à fluide d'un système fluidique et système fluidique - Google Patents

Procédé et appareil de commande destiné à détecter des bulles dans une chambre à fluide d'un système fluidique et système fluidique Download PDF

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Publication number
WO2019015937A1
WO2019015937A1 PCT/EP2018/067651 EP2018067651W WO2019015937A1 WO 2019015937 A1 WO2019015937 A1 WO 2019015937A1 EP 2018067651 W EP2018067651 W EP 2018067651W WO 2019015937 A1 WO2019015937 A1 WO 2019015937A1
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Prior art keywords
measuring
intensity
fluid chamber
detection signal
section
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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PCT/EP2018/067651
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German (de)
English (en)
Inventor
Tino Frank
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Robert Bosch GmbH
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Robert Bosch GmbH
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Filing date
Publication date
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Publication of WO2019015937A1 publication Critical patent/WO2019015937A1/fr
Anticipated expiration legal-status Critical
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/15—Preventing contamination of the components of the optical system or obstruction of the light path
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G01N2021/054—Bubble trap; Debubbling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/15—Preventing contamination of the components of the optical system or obstruction of the light path
    • G01N2021/155—Monitoring cleanness of window, lens, or other parts
    • G01N2021/157—Monitoring by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/128—Alternating sample and standard or reference part in one path
    • G01N2201/1281—Reflecting part, i.e. for autocollimation

Definitions

  • the invention relates to a device or a method according to the preamble of the independent claims.
  • the subject of the present invention is also a computer program.
  • Microfluidic systems allow the analysis of small quantities of samples with high sensitivity. Automation, miniaturization and parallelization also allow the reduction of manual steps and errors.
  • a fluorescence, a chemiluminescence or an optical density can be used as a readout signal.
  • the evaluation of such data may be hampered by air bubbles that may arise in the microfluidic channels under various circumstances. Bubbles should therefore be avoided as far as possible by adapting the hardware or the process flows.
  • a method for detecting bubbles in a fluid chamber of a fluidic system comprising the following steps:
  • the intensity gradient represents a course of an intensity measured in the measuring section using an optical sensor
  • a fluidic system for example, a microfluidic system for analyzing sample quantities based on a fluorescence, a
  • Chemiluminescence or an optical density can be understood.
  • the fluidic system may be a lab-on-a-chip system.
  • Under a bubble for example, an air bubble or other gas bubble can be understood.
  • a fluid chamber can be understood, for example, to mean a depression in a substrate layer of the fluidic system, wherein the depression can be covered by an approximately pneumatically or hydraulically deflectable membrane layer.
  • the measuring field can be, for example, latticed with a plurality of rectangular or
  • a measuring section can be understood as a subfield of the measuring field.
  • the measuring field can extend at least in sections beyond an edge of the fluid chamber and thus, for example, also comprise a measuring section located outside the fluid chamber.
  • the measuring field may be larger or smaller in area than the fluid chamber.
  • the measuring field and the fluid chamber can be the same size in terms of area.
  • An optical sensor can be understood as meaning a sensor for measuring a radiation intensity of an electromagnetic radiation, in particular of light. The intensity can thus be understood to mean a radiation intensity or a light intensity. Under an intensity course can
  • Intensity Verl on by a determined using intensity average cross-section or by a profile derived from the cross profile to be represented.
  • the measured intensity may drop abruptly when a bubble in the fluid chamber is scanned.
  • the detection signal by comparing the numerical value with a
  • Threshold value are generated.
  • a slope change may be understood to mean a transition from a positive to a negative slope, a transition from a negative to a positive slope, a transition from a zero slope to a positive slope, or a transition from a zero slope to a negative slope.
  • the slope change can also be referred to as zero or zero crossing.
  • a detection signal can be understood to mean an output signal which indicates the presence of at least one bubble in the measuring section.
  • the detection signal is generated if the numerical value represents at least two changes in slope of the intensity profile in the measuring section associated with a sign change.
  • the approach presented here is based on the recognition that bubbles in a fluid chamber of a fluidic system can be detected reliably and automatically by evaluating a slope of a curve of an intensity measured during scanning of the fluid chamber by means of a corresponding software processing. As a result, disruptive or distorting influences due to bubbles in the evaluation of opto-fluidic data with respect to the fluid chamber can be avoided. According to one embodiment, not only bubbles in an evaluation volume can be detected by such a software routine, but also a measuring field, English region of interest, can be adjusted so that no more bubbles are measured, or a corresponding input for
  • a bubble segmentation can be omitted.
  • the segmentation of bubbles in optofluidic evaluations can be technically very complicated and compute-intensive.
  • the method presented here does not require a high one
  • the measurement field can be determined according to an embodiment before the measurement and then the actual Measurement be triggered. This is an advantage because no image data is stored, but is averaged on a readout device and only
  • the local evaluation of a bubble-free measuring field allows images to be evaluated locally and quickly, and only numerical values are transmitted and stored. This allows a quick completion of the entire process.
  • the algorithm can also be integrated into the fluidic process.
  • the algorithm allows the system itself to detect if bubbles are present in chambers.
  • a fluid handling system for example, and it can be carried out as long as appropriate fluid routines until no more air bubbles are present or only a minimum amount of air bubbles is present, an adjustment of the measuring field or a further processing in the fluidic process allowed.
  • a feedback system via bladder control allows a dynamic fluid flow.
  • Non-robust processes such as dissolving lyophilized beads, d. H. dry upstream reagents, so can be driven dynamically.
  • Vent steps can be repeated several times until a
  • control images can be taken repeatedly and, if necessary, steps for
  • Bubble removal be installed before further steps are performed. As an example, the start of a PCR is given. If many bubbles are to be detected before a PCR, for example, a bubble removal routine is run before the actual PCR starts. Through an adapted, bubble-free measuring field, the strength of a
  • Light source and an exposure time when recording in a feedback loop to be adjusted are adjusted.
  • Drive signal for driving at least one actuator of the fluidic system can be output using the detection signal.
  • An actuator can be understood, for example, as a pneumatic or hydraulic control unit, for example in the form of a pump or a valve.
  • the fluidic system can be controlled selectively in response to the presence of bubbles, for example, with the aim of eliminating the bubbles.
  • the steps of measuring, determining and generating may be repeated at least once more after the step of outputting. This can increase the efficiency of the process.
  • the detection signal is generated in the step of generating, if the numerical value represents at least two slope changes of the intensity profile associated with a change of sign. This can be done with high reliability on the presence of a bubble in the
  • Measuring section are closed.
  • the method may comprise a step of comparing the numerical value with at least one reference value assigned to the measuring section.
  • the detection signal may be generated depending on a result of the comparing.
  • a reference value may, for example, be understood as meaning a value of a reference intensity representing a bubble or a fluid to be measured.
  • the measurement field may be reduced to produce the changed measurement field. This can reduce the likelihood that bubbles that have not yet been detected will enter the measuring field when changing the measuring field.
  • the reference measuring section may represent a measuring section of the measuring field located outside the fluid chamber and the reference curve may represent a curve of the intensity measured in the reference measuring section using the optical sensor. Accordingly, in the step of generating, the detection signal using the
  • a reference section may, for example, be understood to mean an edge section adjoining the fluid chamber. Also by this embodiment, the reliability of the
  • the measuring section may be scanned in a first scanning direction and a second scanning direction.
  • the intensity profile can be formed using mean values of an intensity measured in the second scanning direction, each of which is assigned to a scanning position in the first scanning direction.
  • the first scanning direction a scanning direction and a second scanning direction.
  • the intensity profile can be measured quickly, precisely and with little computational effort. It is particularly advantageous if, in the step of measuring, at least one further intensity profile is measured using the measuring field.
  • the measuring field can subdivide the section of the fluid chamber into at least one further measuring section, and the further intensity profile can be measured in the further measuring section using the optical sensor
  • the measuring field can have, in addition to the measuring section, for example, a plurality of further measuring sections, wherein the measuring section and the further measuring sections can be arranged in a grid, for example, a checkerboard, in order to form the measuring field.
  • This method can be implemented, for example, in software or hardware or in a mixed form of software and hardware, for example in a control unit.
  • a software routine can be integrated into an optomic-fluidic system by means of a programming language.
  • the approach presented here also provides a control unit which is designed to implement the steps of a variant of a method presented here
  • control unit can have at least one arithmetic unit for processing signals or data, at least one memory unit for storing signals or data, at least one interface to a sensor or an actuator for reading sensor signals from the sensor or for outputting of control signals to the actuator and / or at least one
  • the arithmetic unit may be, for example, a signal processor, a microcontroller or the like, wherein the memory unit is a flash memory, an EPROM or a
  • the magnetic storage unit can be.
  • the communication interface can be designed to read or output data wirelessly and / or by line, wherein a communication interface that can read or output line-bound data, for example, electrically or optically read this data from a corresponding data transmission line or output in a corresponding data transmission line.
  • a control device can be understood as meaning an electrical device which processes sensor signals and outputs control and / or data signals in dependence thereon.
  • the control unit may have an interface, which may be formed in hardware and / or software. In a hardware training, the interfaces may for example be part of a so-called system ASICs, the various functions of the
  • Control unit includes. However, it is also possible that the interfaces are separate, integrated circuits or at least partially discrete
  • the interfaces may be software modules that are present, for example, on a microcontroller in addition to other software modules.
  • the approach presented here also creates a fluidic system with the following
  • Characteristics a fluid chamber; and a controller according to a preceding embodiment.
  • a computer program product or computer program with program code which can be stored on a machine-readable carrier or storage medium such as a semiconductor memory, a hard disk memory or an optical memory and for the implementation, implementation and / or Triggering the steps of the method according to one of the above
  • Fig. 1 is a schematic representation of a fluidic system according to a
  • Fig. 2 is a diagram illustrating an intensity profile in a
  • Fig. 3 is a schematic representation of a fluidic system according to a
  • Fig. 4 is a diagram illustrating an intensity profile in a
  • Fig. 5 is a schematic representation of a fluidic system according to a
  • Fig. 6 is a diagram illustrating an intensity profile in a
  • FIG. 7 is a schematic representation of a measuring field, a transverse profile and a digital profile, created by a control device according to a
  • FIG. 8 shows a schematic representation of a measuring field, a transverse profile and a digital profile, created by a control device according to FIG.
  • Fig. 9 is a schematic representation of a measuring field and a changed
  • Measuring field created by a control unit according to a
  • FIG. 10 is a schematic representation of a control device according to a
  • FIG. 11 is a flowchart of a method according to a
  • the fluidic system 100 includes a
  • Fluid chamber 102 for holding a fluid to be examined, here indicated by hatching, an optical sensor 104 for measuring an intensity in the fluid chamber 102, such as fluorescence, chemiluminescence or optical density of the fluid, and a controller 106 for evaluating the by the optical Sensor 104 measured intensity.
  • the control unit 106 is designed to overlap at least a portion of the fluid chamber 102 with a measuring field 110, which is here rectangular by way of example.
  • Measuring field 110 subdivides the section of fluid chamber 102 into at least one measuring section 112. According to this exemplary embodiment, measuring field 110 is identical in area to measuring section 112. Alternatively, measuring field 110 comprises a plurality of others in addition to measuring section 112
  • Measuring sections which together with the measuring section 112, for example, form a checkered grid, wherein the measuring sections subfields of
  • the optical sensor 104 is designed to scan the fluid chamber 102 in the measuring section 112 in at least one scanning direction and to transmit corresponding scanning signals 114 to the control device 106.
  • the control unit 106 is designed to determine an intensity profile assigned to the measuring section 112 using the scanning signals 114 and the measuring field 110 and to output a corresponding intensity profile
  • the controller 106 Based on the number of slope changes, the controller 106 is able to detect a bubble 116 located in the measuring section 112. In this case, the control unit 106 generates a bubble 116 representing
  • the control unit 106 is designed to scan the measuring field 110 or the measuring section 112 using the optical sensor 104 in a first scanning direction x and a second scanning direction y. In this case, the control unit 106 determines the intensity course on the basis of average values of an intensity measured in the second scanning direction y. The mean values are each assigned to a scanning position in the first scanning direction x.
  • Fig. 1 the basic principle of a bubble detection is shown.
  • a transverse profile of the intensity in the defined measuring field 110 is calculated.
  • Cross-section consists, for example, of averaged pixel values in the y-direction for each pixel position in the x-direction. If a bubble is present, the intensity suddenly decreases, where the bubble is located in the x-direction, as can be seen from FIG.
  • FIG. 2 shows a diagram for illustrating an intensity profile in a fluidic system from FIG. 1.
  • the intensity profile is represented by a curve 200, which here has a curve 202, wherein the curve 202 indicates a sudden drop in intensity in the area of the bubble.
  • FIG. 3 shows a schematic representation of a fluidic system 100 according to one exemplary embodiment.
  • the measuring field 110 according to this exemplary embodiment is larger in area than the fluid chamber 102, so that the measuring field 110 projects beyond an edge of the fluid chamber 102 on at least one side of the fluid chamber 102.
  • Section of the measuring field 110 functions as a reference measuring section 300 for measuring a reference curve of the intensity outside the fluid chamber 102. Accordingly, the control device is designed to block bubbles in the
  • Fluid chamber 102 with additional use of the reference curve to detect.
  • FIG. 4 shows a diagram for illustrating an intensity profile in a fluidic system from FIG. 3.
  • the curve 200 is shown with a first curve section 400, which has a reference section, ie in the background, measured and acting as a reference curve intensity curve
  • Figures 3 and 4 show how a distinction can be made between background and signal. A part of the measuring field 110 is also outside the
  • Fluid chamber 102 From the signal rise in the transition from the background into the chamber area, the optical stroke of the signal can be detected.
  • FIG. 5 shows a schematic representation of a fluidic system 100 according to one exemplary embodiment.
  • the fluid chamber 102 has according to this
  • Embodiment a parabolic surface profile.
  • FIG. 6 shows a diagram for illustrating an intensity profile in a fluidic system from FIG. 5.
  • the curve 200 here has a parabolic profile.
  • FIGS. 5 and 6 show how the transverse profile behaves as a function of different chamber heights. The higher the fluid chamber 102, the stronger the signal will be. Therefore, a measurement field that is part of the
  • FIG. 7 shows a schematic representation of a measuring field 110, a
  • the measuring field 110 also called region n-of-interest grid, according to this exemplary embodiment comprises the measuring section 112 and, by way of example, eight further measuring sections 704, which form a grid matrix of three times three equally sized measuring sections, in this case square measuring sections.
  • the measurement sections can also also be used
  • the cross profile 700 represents the intensity profiles measured in the respective measuring sections 112, 704, which for illustration in the form of corresponding curves into the respective ones
  • the digital profile 702 represents the Sign change in the respective measuring sections 112, 704 in the form of rectangular curves.
  • FIG. 8 shows a schematic representation of a measuring field 110, a
  • FIG. 8 shows the transverse profile 700 and the digital profile 702 in the presence of bubbles 116, here by way of example two bubbles, in the fluid chamber 102.
  • Figures 7 and 8 show the technical process and the actual core of the approach presented here.
  • a bubble-free fluid chamber is shown schematically, while in Fig. 8, a case is shown with bubbles.
  • a grid as the measuring field 110 on the
  • Fluid chamber 102 is laid, wherein the measuring field 110, the fluid chamber 102 into a plurality of rectangular measuring sections 112, 704 divided.
  • the exact number of measuring sections depends on the size of the area to be measured and the number of pixels in the resulting measuring sections, also called subfields.
  • a digital profile is a sign of the first derivation of a transverse profile.
  • a positive slope is represented as 1 and a negative slope or slope as -1, as can be seen from the following matrix of numerical values representing the slope to the digital profile 702 shown in FIG.
  • Such a matrix can also be called a quality matrix.
  • a number of zeroes is then determined.
  • a zero is meant a transition from a positive to a negative slope or vice versa.
  • the number of zeros is additionally calculated with the sign of the first point from the respective subprofile to show whether the profile starts with signal or without signal.
  • the number of zeroes is then stored as a numerical value in the quality matrix, which for example has the same dimension as the measuring field 110.
  • the quality matrix for the case shown in FIG. 8 is, for example:
  • the quality matrix for a bubble-free chamber such as the fluid chamber 102 shown in Fig. 7, has a unique value for each entry. This should only
  • Chamber intensity maximum and have the transition from chamber to background. If bubbles are present, additional node transitions occur, as shown in FIG.
  • the quality matrix is compared, for example, with a desired matrix. In all fluid chambers where the difference between
  • FIG. 9 shows a schematic illustration of a measuring field 110 and a changed measuring field 900, created by a control device according to FIG.
  • Embodiment such as a previously described with reference to Figures 1 to 8 controller.
  • the measuring field 110 is formed similarly to FIGS. 7 and 8 as a grid matrix with three by three measuring sections.
  • the control device is designed to be the measuring field
  • the measuring field 110 can be dynamic.
  • FIG. 10 shows a schematic representation of a control device 106 according to an exemplary embodiment.
  • the controller 106 is
  • the control unit 106 includes a measuring unit 1010, the
  • History information 1012 to determine at least one numerical value 1022, the number of associated with a sign change
  • Generation unit 1030 is configured to receive the numerical value 1022 from the determination unit 1020 and generate the detection signal 118, as long as the numerical value 1022 represents the presence of a bubble in the fluid chamber. This is the case, for example, if the numerical value 1022 has at least two signs associated with a sign change
  • FIG. 11 shows a flowchart of a method 1100 according to FIG.
  • the method 1100 for detecting a bubble in a fluid chamber of a fluidic system can be carried out, for example, by a control device described above with reference to FIGS. 1 to 10.
  • the intensity profile is measured, for example by taking an image of the fluid chamber.
  • the numerical value representing the number of slope changes is determined using the intensity profile. This numerical value is evaluated in a step 1130 to decide whether the measuring field contains bubbles or not. Depending on the result of this evaluation, the detection signal is generated.
  • the measurement field is first formed.
  • Detection signal in step 1130 generates the changed, this time bubble-free measuring field.
  • the image recording for measuring the intensity profile is continued with the changed measuring field.
  • step 1110 is performed again to re-measure the intensity profile. If the bubbles are at least largely eliminated, for example, the normal operation of the fluidic system is continued.
  • Fig. 11 shows a general instruction flow in a microfluidic drive system.
  • the system accordingly comprises an optical unit that records images and a fluidic, pneumatic
  • BubbleCheck starts with an image capture. Then, a default measurement field is divided into a 3x3 grid in which an intensity profile is calculated from pixel values and bubbles are determined based on the sign changes of the slope of the intensity profile.
  • the individual grids are on the
  • BubbleCheck is used to check for bubbles.
  • a program is interposed which can remove bubbles.
  • BubbleCheck will called again until no or very few bubbles are present, or the program is stopped after a certain number of cycles when bubbles multiply or there is too much air in the system
  • an exemplary embodiment comprises a "and / or" link between a first feature and a second feature, then this is to be read so that the embodiment according to one embodiment, both the first feature and the second feature and according to another embodiment either only first feature or only the second feature.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
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Abstract

L'invention concerne un procédé de détection de bulles (116) dans une chambre à fluide (102) d'un système fluidique (100). Dans le procédé, au moins un profil d'intensité est mesuré à l'aide d'un champ de mesure (110) qui subdivise au moins une partie de la chambre à fluide (102) en au moins une partie de mesure (112). Le profil d'intensité représente l'évolution d'une intensité mesurée dans la partie de mesure (112) à l'aide d'un capteur optique (104). Dans une autre étape, au moins une valeur numérique est déterminée qui représente un nombre de changements de pente du profil d'intensité qui est associé à un changement de signe. Enfin, un signal de détection (118) représentant une bulle (116) est généré à l'aide la valeur numérique.
PCT/EP2018/067651 2017-07-17 2018-06-29 Procédé et appareil de commande destiné à détecter des bulles dans une chambre à fluide d'un système fluidique et système fluidique Ceased WO2019015937A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102017212196.3A DE102017212196A1 (de) 2017-07-17 2017-07-17 Verfahren und Steuergerät zum Detektieren von Blasen in einer Fluidkammer eines fluidischen Systems und fluidisches System
DE102017212196.3 2017-07-17

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