WO2019201990A1 - Plaque à orifices multiples pour sac de bioprocédé - Google Patents

Plaque à orifices multiples pour sac de bioprocédé Download PDF

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Publication number
WO2019201990A1
WO2019201990A1 PCT/EP2019/059917 EP2019059917W WO2019201990A1 WO 2019201990 A1 WO2019201990 A1 WO 2019201990A1 EP 2019059917 W EP2019059917 W EP 2019059917W WO 2019201990 A1 WO2019201990 A1 WO 2019201990A1
Authority
WO
WIPO (PCT)
Prior art keywords
bag
bioreactor
plate
multiport
ports
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2019/059917
Other languages
English (en)
Inventor
Michael Jason MILLER
Hanna-Leena SAUKKONEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences Corp filed Critical GE Healthcare Bio Sciences Corp
Priority to US16/980,683 priority Critical patent/US12152227B2/en
Priority to EP19720081.9A priority patent/EP3781667A1/fr
Priority to AU2019254568A priority patent/AU2019254568B2/en
Priority to JP2020558019A priority patent/JP7584842B2/ja
Priority to CN201980026722.5A priority patent/CN112074594A/zh
Publication of WO2019201990A1 publication Critical patent/WO2019201990A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals

Definitions

  • the present invention relates to the field of single-use bioprocess bags, and more specifically to a multiport plate for a single-use bioreactor bag.
  • Disposable systems are flexible and cost-effective and cleaning processes may be reduced or eliminated.
  • Traditional systems comprise re-usable wetted parts in fluid contact which are typically built as stainless steel installations (tubes and vessels), which need to be cleaned or sterilized in between processes or batches. Sterilization is typically done by steam sterilization, which requires technical infrastructure and is a complex and time-consuming process.
  • disposable components in disposable systems provide surfaces in fluid contact that are preferably pre-sterilized and pre-qualified to all regulatory requirements.
  • Disposable or single-use systems are replaced in between processes to eliminate cleaning and sterilization issues. Due to lower complexity in systems and auxiliary systems, disposable systems are therefore easy to adapt to different production purposes and facilities. Further, it is easy and less costly to change a product line compared to traditional equipment. Disposable systems may provide also improved reliability as well as product and operator safety in biopharmaceutical processing.
  • a flexible bioprocess bag refers to a bag or pouch made of walls of similar structure preferably assembled by welding. These walls may be made of a mono or multilayer film including or not a barrier layer based on a barrier polymer like ethylene vinyl alcohol polymer (EVOH). Generally, these films may have an inner layer (in contact with the contents of the bag when filled) based on a polyolefin, preferably an ultra-low density polyethylene, pref. medical grade (ULDPE).
  • the bag may be of cylindrical shape.
  • cylindrical flexible bioprocess bags are difficult to manufacture, the cylindrical shape can be approximated and achieved by welding of multiple film panels of suitable size and shape.
  • the flexible bioprocess bags can equally have cubic or parallelepiped shape.
  • processing and pre-conditioning steps need to be performed within these bags such as for example pre-sterilization.
  • One type of mixing system in which such containers or bags can be used is a bioreactor system in which cells or microorganisms can grow.
  • the bags are provided as closed and pre- sterilized components in order to avoid any contamination or inhibition in the growth of the microorganisms or cells intended for cultivation in the bioreactor.
  • Mixing systems may comprise a support or vessel which supports or houses a disposable bag or container of the above-mentioned type.
  • the vessel may also be a tank-type support which has a substantially cylindrical or parallelepiped form, for example substantially circular cylindrical and is made of rigid material such as stainless steel to provide sufficient support for the flexible bioprocess bag or container, for example of a kind used in Xcellerex XDRTM Single- use bioreactors.
  • the Xcellerex bioreactor is an example of a mixing system comprising an active mixing element, here a rotating impeller.
  • the flexible container or bag is placed inside the vessel in an accurate manner so that for example different pipelines or tubes, mixers and sensors can be connected to the bag properly and accurately.
  • WO 2005/118771 A2 discloses a disposable system of this kind.
  • a bioreactor bag has many ports for connection of various tubing and sensors. These ports are typically located on the top of the bag and on a side wall of the bag, where the connections to the side wall ports pass through an opening in the rigid support vessel.
  • the number of ports required can be different for different applications of the bag and during installation of the bag in the vessel, moving the different ports to a position in front of the opening can be complicated.
  • One aspect of the invention is to provide an easily installed single-use bioreactor bag, which can easily be adapted to different cell cultivation applications during manufacturing.
  • a bioreactor bag comprising a rigid multiport plate sealed to a side wall of the bioreactor bag, wherein the multiport plate comprises a plurality of ports.
  • different ports can either be fluidically connected to an interior volume of the bag or they can be sealed off from the interior volume.
  • a further advantage of the invention is that multiple ports can be placed at closer distance to each other than when they are separately welded to the bag film.
  • a further aspect of the invention provides a method of manufacturing the bag discussed above.
  • a yet further aspect of the invention provides a method of installing the bag discussed above in a rigid support vessel.
  • Fig. 1 shows two prior art single ports mounted side by side.
  • Fig. 2 shows a multiport plate of the invention.
  • Fig. 3 shows the mounting of a multiport plate in a bioreactor bag of the invention.
  • Fig. 4 shows a view from the inside of the bag of a multiport plate in a bioreactor bag of the invention, with a) square sealing welds around the ports and b) circular sealing welds.
  • Fig. 5 shows a photograph of a prototype multiport plate, with tubing connected to four of the ports.
  • Fig. 6 shows a rigid support vessel with a bioreactor bag of the invention.
  • the invention discloses a single-use flexible bioreactor bag 1 comprising a rigid multiport plate 2 sealed to a side wall 3 of the bioreactor bag.
  • the multiport plate comprises a plurality of ports 4 wherein at least a portion 5 of the plurality of ports can suitably be fluidically connected to an interior volume 8 of the bioreactor bag.
  • the plurality of ports are suitably fluidically connectable to one or more exterior appliances, which can e.g. include supplies of liquids and/or gases, sampling devices, cell separation devices, sensors etc. These connections can be done via fittings 6, e.g. barbed tubing connectors, protruding from the multiport plate e.g. through apertures 7 in the bag side wall.
  • the multiport plate 2 suitably comprises a base plate 19, with an inside 11 and an outside 20.
  • the base plate can e.g.
  • the plate comprises a plurality of ports 4, each port suitably comprising an opening through the base plate and, extending from the outside 20, a fitting 6 surrounding the opening.
  • the fitting can e.g. be a hose barb connector, but can also be another type of fitting suitable for connecting tubing (e.g. a straight tubular fitting, hose tail or other hose fittings known in the art).
  • a portion 5 of the plurality of ports are fluidically connected to an interior volume 8 of the bioreactor bag.
  • the fluidical connection can suitably be via apertures 9 formed in a back film 10 covering an inside 11 of the multiport plate, i.e. the side facing the bag interior volume, in registry with ports 5 in the multiport plate.
  • no apertures are formed, leaving the back film sealing off these ports.
  • the back film can then be sealed 13 to the multiport plate around each aperture to prevent cross-talk between the ports and to avoid stagnant volumes.
  • the seals may e.g. be rectangular or circular.
  • a perimeter 14 of the back film can be sealed to the bag side wall, forming a pouch containing the multiport plate.
  • the bag may then be assembled by welding together a number of film panels, including the side wall panel 3 comprising the multiport plate.
  • the sealing of the back film to the multiport plate and to the bag side wall can suitably be done by welding to avoid the use of adhesives that may produce undesirable leachables.
  • the multiport plate and both sides of the back film can be made from materials that are weldable to each other and to the inside of the bag film.
  • they can comprise polyethylene or ethylene copolymers such as ethylene- vinyl acetate (EVA), either alone or in the form of blends with e.g. cycloolefin copolymers (COC). All materials preferably meet the USP VI requirements for biocompatibility.
  • the multiport plate may further comprise one or more guides 15 for fitting the plate in a window 16 (also known as an alcove or opening) of a rigid support vessel 17.
  • the guides 15 can e.g. be ridges or ribs extending from an outer surface of the multiport plate and arranged to mate with the frame of the window 16.
  • the guides may further comprise snap fit elements (not shown) for easy attachment to the window.
  • the guides protrude through slits or openings 18 formed in the side wall of the bag.
  • the invention provides a method of manufacturing the bioreactor bag as discussed above. This method comprises the steps of:
  • the multiport plate may comprise one or more guides for fitting the plate in a window of a rigid support vessel.
  • step c) may further comprise forming (e.g. punching or cutting) a set of slits or openings for the guides in the bag wall panel and in step e) the guides protrude through the slits or openings.
  • the invention provides a method of installing the bag as discussed above in a rigid support vessel. This method comprises the steps of: a) providing a bioreactor bag as discussed above and a rigid support vessel comprising a window;
  • step c) may further comprise mating the guides with a frame of the window. If the guides comprise snap fit elements, step c) may also comprise snap-fitting the multiport plate into the window frame.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un sac souple de bioréacteur à usage unique comprenant une plaque rigide à orifices multiples scellée à une paroi latérale dudit sac de bioréacteur, ladite plaque à orifices multiples comprenant une pluralité d'orifices. L'invention concerne en outre un procédé de fabrication du sac et un procédé d'installation du sac dans un récipient de support rigide.
PCT/EP2019/059917 2018-04-20 2019-04-17 Plaque à orifices multiples pour sac de bioprocédé Ceased WO2019201990A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US16/980,683 US12152227B2 (en) 2018-04-20 2019-04-17 Multiport plate for a bioprocess bag
EP19720081.9A EP3781667A1 (fr) 2018-04-20 2019-04-17 Plaque à orifices multiples pour sac de bioprocédé
AU2019254568A AU2019254568B2 (en) 2018-04-20 2019-04-17 Multiport plate for a bioprocess bag
JP2020558019A JP7584842B2 (ja) 2018-04-20 2019-04-17 バイオプロセスバッグ用の多ポートプレート
CN201980026722.5A CN112074594A (zh) 2018-04-20 2019-04-17 用于生物过程袋的多端口板

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862660295P 2018-04-20 2018-04-20
US62/660,295 2018-04-20

Publications (1)

Publication Number Publication Date
WO2019201990A1 true WO2019201990A1 (fr) 2019-10-24

Family

ID=66323835

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2019/059917 Ceased WO2019201990A1 (fr) 2018-04-20 2019-04-17 Plaque à orifices multiples pour sac de bioprocédé

Country Status (6)

Country Link
US (1) US12152227B2 (fr)
EP (1) EP3781667A1 (fr)
JP (1) JP7584842B2 (fr)
CN (1) CN112074594A (fr)
AU (1) AU2019254568B2 (fr)
WO (1) WO2019201990A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102019107622B4 (de) * 2019-03-25 2021-02-25 Sartorius Stedim Biotech Gmbh Sensorpositioniersystem für eine bioprozesstechnische Anlage

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005118771A2 (fr) 2004-06-04 2005-12-15 Xcellerex, Inc. Systemes de bioreacteurs jetables et procedes associes
CN201737946U (zh) * 2010-04-30 2011-02-09 惠倪 动物细胞培养用的多边形塑料培养袋
WO2012082974A1 (fr) * 2010-12-17 2012-06-21 Rosemount Analytical, Inc. Intégration d'un capteur de ph à un bioréacteur/mélangeur à usage unique
EP2829598A2 (fr) * 2013-07-24 2015-01-28 Pall Corporation Joint de sonde de capteur
WO2015077663A1 (fr) * 2013-11-21 2015-05-28 Distek Inc. Bioréacteurs jetables et procédés pour leur construction et leur utilisation

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Publication number Priority date Publication date Assignee Title
US5350080A (en) * 1993-02-10 1994-09-27 Hyclone Laboratories Multi-access port for use in a cell culture media system
US6086574A (en) * 1997-11-21 2000-07-11 Hyclone Laboratories, Inc. Fluid delivery systems with diptube connector
US7879599B2 (en) * 2005-04-22 2011-02-01 Hyclone Laboratories, Inc. Tube ports and related container systems
US8075189B2 (en) * 2006-06-21 2011-12-13 Bosch Pouch Systems Ag Method for attaching a spout to a flexible film pouch
JP5405313B2 (ja) * 2006-12-20 2014-02-05 テルモ ビーシーティー、インコーポレーテッド 複合液体を少なくとも２つの成分に分離するための機器および方法
US9090445B2 (en) * 2010-10-22 2015-07-28 Daniel Py Pouch connector and related method
EP2809770A1 (fr) * 2012-02-02 2014-12-10 Corning Incorporated Systèmes de culture cellulaire
CN113046217B (zh) * 2012-04-18 2024-09-06 生命科技股份有限公司 用于气体流与液体质量传递的方法和设备
US10617070B2 (en) * 2014-10-06 2020-04-14 Life Technologies Corporation Methods and systems for culturing microbial and cellular seed cultures
JP6998771B2 (ja) * 2015-04-13 2022-01-18 ローズマウント インコーポレイテッド 複数のセンサを有する単回使用バイオリアクタポート
EP3460036B1 (fr) * 2017-09-22 2020-02-12 Sartorius Stedim Biotech GmbH Échantillonnage de sonde stérile pour un récipient à usage unique

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005118771A2 (fr) 2004-06-04 2005-12-15 Xcellerex, Inc. Systemes de bioreacteurs jetables et procedes associes
CN201737946U (zh) * 2010-04-30 2011-02-09 惠倪 动物细胞培养用的多边形塑料培养袋
WO2012082974A1 (fr) * 2010-12-17 2012-06-21 Rosemount Analytical, Inc. Intégration d'un capteur de ph à un bioréacteur/mélangeur à usage unique
EP2829598A2 (fr) * 2013-07-24 2015-01-28 Pall Corporation Joint de sonde de capteur
WO2015077663A1 (fr) * 2013-11-21 2015-05-28 Distek Inc. Bioréacteurs jetables et procédés pour leur construction et leur utilisation

Non-Patent Citations (1)

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Title
DATABASE WPI Week 201127, Derwent World Patents Index; AN 2011-D52901, XP002792406 *

Also Published As

Publication number Publication date
EP3781667A1 (fr) 2021-02-24
AU2019254568B2 (en) 2024-07-25
JP7584842B2 (ja) 2024-11-18
JP2021521802A (ja) 2021-08-30
AU2019254568A1 (en) 2020-10-08
US20210024864A1 (en) 2021-01-28
US12152227B2 (en) 2024-11-26
CN112074594A (zh) 2020-12-11

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