WO2020056808A1 - Dispositif et système de détection de vert d'indocyanine dans le sang - Google Patents

Dispositif et système de détection de vert d'indocyanine dans le sang Download PDF

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Publication number
WO2020056808A1
WO2020056808A1 PCT/CN2018/109558 CN2018109558W WO2020056808A1 WO 2020056808 A1 WO2020056808 A1 WO 2020056808A1 CN 2018109558 W CN2018109558 W CN 2018109558W WO 2020056808 A1 WO2020056808 A1 WO 2020056808A1
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WIPO (PCT)
Prior art keywords
blood
green
shunt tube
detecting
photodetector
Prior art date
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Ceased
Application number
PCT/CN2018/109558
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English (en)
Chinese (zh)
Inventor
刘旭
刘吉奎
黄茜
姚思宇
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Peking University Shenzhen Hospital
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Peking University Shenzhen Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201821554495.2U external-priority patent/CN209416918U/zh
Priority claimed from CN201811107298.0A external-priority patent/CN109100342A/zh
Application filed by Peking University Shenzhen Hospital filed Critical Peking University Shenzhen Hospital
Publication of WO2020056808A1 publication Critical patent/WO2020056808A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence

Definitions

  • the invention relates to the technical field of biomedical in vitro detection, and in particular, to a device and system for detecting indocyanine green in blood.
  • Indocyanine green (Indocyanine green) is immediately injected into the body through the vein, and immediately binds to plasma proteins. It is quickly distributed in the blood vessels of the whole body with the blood circulation. In the middle, enter the intestine through the biliary tract, and excrete with the feces. Due to rapid excretion, about 97% of normal people are excluded from the blood after 20 minutes of intravenous injection, do not participate in in vivo chemical reactions, no enterohepatic circulation, no lymphatic reflux, and are not excreted from the kidney and other extrahepatic organs. The retention of indocyanine green in the blood after entering the body can well reflect the liver reserve function of the body. Therefore, the determination of the residual amount of indocyanine green in the blood after injection into the body will detect the liver's metabolic capacity and related diseases. Diagnosis is important.
  • a pulse photometry method is disclosed in the related technology (Wu Jing. (2015). Noninvasive PDD to detect liver effective blood flow in the short-term and prognostic value of patients with chronic acute liver failure. Master's thesis, Guiyang Medical College), in vitro The density of two different light-absorbing substances in the blood was measured and compared, and the indocyanine green concentration injected into the body was analyzed in real time for the diagnosis of liver function reserve and related levels.
  • individual differences between organisms for example: differences in the skin and muscles of the organism cause different degrees of fluorescence attenuation when passing through these tissues; and although this difference can be calibrated, it is difficult to achieve accuracy, And time consuming
  • the related equipment needs to be frequently calibrated and parameter modified.
  • the present invention aims to solve at least one of the technical problems in the related technology. Therefore, the object of the present invention is to provide an indole cyanide green detection device and system.
  • a device for detecting indolocyanine green in blood includes:
  • a blood shunt tube is a transparent material, and is used to connect the organism to be tested, so that the blood in the blood vessel of the organism to be tested is shunted through the blood shunt tube;
  • a photodetector the photodetector is located at a predetermined distance from the blood shunt tube, and is used to receive fluorescence from indole cyanide green in the blood and convert it into an electrical signal;
  • a data acquisition device which is connected to the photodetector and is used to acquire the electrical signal.
  • the device for detecting indocyanine green in blood may also have the following additional technical features:
  • one end of the blood shunt tube is adapted to be connected to a first position of a blood vessel of the organism to be detected, and the other end of the blood shunt tube is adapted to be connected to a first position of a blood vessel of the organism to be detected. Two positions, so that the blood flowing out of the first position returns to the blood vessel from the second position after passing through the blood shunt tube.
  • a peristaltic pump is further provided.
  • the peristaltic pump is provided on the blood shunt tube and is used for conveying blood in the blood shunt tube.
  • a dark room is further included, and the photodetector is disposed in the dark room to reduce interference of external ambient light.
  • a first needle is provided on one end of the blood shunt tube, and a second needle is provided on the other end of the blood shunt tube.
  • the photodetector is any one of a photomultiplier tube, a silicon photomultiplier, a photodiode, and an avalanche photodiode.
  • the predetermined distance is 0.01 mm to 10 cm.
  • a system for detecting indocyanine green in blood includes:
  • a processing device the processing device is connected to the data acquisition device, and is configured to determine the content of the indole cyanide green according to the electrical signal.
  • a display device is further included, and the display device is connected to the data acquisition device.
  • the electrical signal is a pulse signal
  • the data acquisition device is specifically configured to periodically collect the number of the pulse signals output by the photodetector in a unit time according to a collection time interval.
  • the processing device is specifically configured to calculate the fluorescence intensity according to the number of the pulse signals, and determine the content of the indole cyan green according to the fluorescence intensity.
  • the processing device is further configured to generate indole cyan green data information according to the fluorescence intensity and send it to the display device for display.
  • the indole cyan green data information is about Relationship curve between "fluorescence intensity and measurement time”.
  • a blood shunt tube is used to drain blood from an organism to be detected to the outside of the body, and then a photodetector is used to detect the fluorescence emitted by the blood indocyanine green to generate a response.
  • the data acquisition device collects the electrical signal, and the processing device determines the content of indolocyanine green according to the electrical signal.
  • the detection of the concentration of indolocyanine in blood is realized, and the structure is simple and the detection is convenient; and, Because the blood shunt tube is used to drain blood out of the body for detection, the fluorescence passes through the transparent blood shunt tube and is received by the photodetector without the need to pass through human tissue. Therefore, it can be avoided in related technologies when fluorescence requires traditional body tissue. Absorption and loss, and inaccurate detection results due to individual differences between organisms, as well as the need for correction and parameter revision, etc., the detection results are accurate, high sensitivity, stable and reliable.
  • FIG. 1 is a schematic structural diagram of an indocyanine green detection system in blood according to an embodiment of the present invention.
  • first and second are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as “first” and “second” may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality” is two or more, unless specifically defined otherwise.
  • the terms “installation”, “connected”, “connected”, “fixed” and other terms shall be understood in a broad sense unless otherwise specified and defined, for example, they may be fixed connections or removable connections , Or integrally connected; it can be mechanical or electrical; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements.
  • the specific meanings of the above terms in the present invention can be understood according to specific situations.
  • the "first" or “down” of the second feature may include the first and second features in direct contact, and may also include the first and second features. Not directly, but through another characteristic contact between them.
  • the first feature is “above”, “above”, and “above” the second feature, including that the first feature is directly above and obliquely above the second feature, or merely indicates that the first feature is higher in level than the second feature.
  • the first feature is “below”, “below”, and “below” of the second feature, including the fact that the first feature is directly below and obliquely below the second feature, or merely indicates that the first feature is less horizontal than the second feature.
  • Indocyanine green is a fluorescently labeled drug that is metabolized by the liver. After intravenous injection into the body, the change in the concentration of indocyanine green in the blood directly reflects the liver function of the body. Therefore, the determination of the content of indocyanine green in the blood after injection into the body is of great significance for the detection of the metabolic capacity of the liver and the diagnosis of related diseases.
  • An embodiment of the present invention provides a detection system capable of detecting the content of indocyanine green in the blood.
  • a system for detecting indole cyanine green in blood includes an indole cyanide green detection device 100 and a processing device 200 in blood.
  • the indocyanine green detection device 100 in the blood includes a blood shunt tube 10, a photodetector 11, and a data acquisition device 12.
  • the blood shunt tube 10 is made of a transparent material and is used to connect the living body 400 to be tested, so that the blood in the blood vessel of the living body 400 to be shunted through the blood shunt tube 10.
  • the blood shunt tube 10 is connected to a blood vessel on the organism 400 to be tested, such as a venous blood vessel or an arterial blood vessel. In this way, blood in the blood vessel can flow into the blood shunt outside the body.
  • the blood shunt tube 10 is a transparent material, which can ensure that the fluorescence emitted by indole cyanide green in the blood can be emitted through the blood shunt tube 10.
  • the photodetector 11 is located at a predetermined distance from the blood shunt tube 10, and is used to receive the fluorescence from indole cyanide green in the blood and convert it into an electrical signal.
  • the photodetector 11 may be any one of a photomultiplier tube, a silicon photomultiplier, a photodiode, and an avalanche photodiode.
  • the predetermined distance between the photodetector 11 and the blood shunt tube 10 is 0.01 mm to 10 cm. That is, the photodetector 11 is disposed adjacent to the blood shunt tube 10.
  • the fluorescence emitted by indole cyanide green in the blood can be detected by the photodetector 11 and converted into an electrical signal.
  • the intensity of the electrical signal and the intensity of the fluorescence There is a correlation, for example, the stronger the intensity of the fluorescence, the stronger the intensity of the generated electrical signal.
  • a data acquisition device 12 is connected to the photodetector 11 to collect the electrical signal, and a processing device 200 is connected to the data acquisition device 12 to determine the content of the indole cyanide green according to the electrical signal.
  • the data acquisition device 12 can collect the electrical signals output by the photodetector 11. Since the intensity of fluorescence is related to the content of indole cyanide green, for example, the higher the content of indole cyanide green, the stronger the intensity of the fluorescence it emits. Therefore, the content of indocyanine green can be determined by the processing device 200 based on the electrical signal.
  • the blood shunt tube 10 is used to drain blood from the organism 400 to be detected to the outside, and then the photodetector 11 is used to detect the fluorescence generated by the blood indocyanine green.
  • the data acquisition device 12 collects the electrical signal, and the processing device 200 determines the content of indocyanine green according to the electrical signal. In this way, the detection of the concentration of indocyanine green in the blood is realized, and its structure is simple and convenient.
  • the fluorescence passes through the transparent blood shunt tube 10 and is received by the photodetector 11 without the need to pass through human tissue, so fluorescence in the related art can be avoided Absorption and loss when traditional tissues are needed, and inaccurate detection results due to individual differences between organisms, as well as the need for correction and parameter revision, etc.
  • the detection results are accurate, highly sensitive, stable and reliable.
  • one end of the blood shunt tube 10 is adapted to be connected to a first position of a blood vessel of the organism 400 to be tested, and the other end of the blood shunt tube 10 is adapted to be connected to a blood vessel of the organism 400 to be tested.
  • the second position is such that the blood flowing out of the first position is returned to the blood vessel from the second position after passing through the blood shunt tube 10.
  • one end of the blood shunt tube 10 may be connected to a first position (proximal end) of a blood vessel of the organism 400 to be tested, and the other end of the blood shunt tube 10 may be connected to the The second position (distal end) of the blood vessel.
  • the blood in the blood vessel can flow from the first position into the blood shunt tube 10.
  • it After passing through the blood shunt tube 10, it returns to the blood vessel from the second position. In this way, the blood can be ensured. Flow back into the organism 400 to be detected to avoid damage to the organism 400 to be detected.
  • the one end of the blood shunt tube 10 is provided with a first needle (not shown), and the other end of the blood shunt tube 10 is provided with a second needle (not shown).
  • the first and second needles can be arterial or venous needles.
  • the first needle is used to penetrate into the first position of the artery or vein
  • the second needle is used to penetrate into the second position of the artery or vein.
  • a peristaltic pump 13 is further provided.
  • the peristaltic pump 13 is disposed on the blood shunt tube 10 and is used for conveying blood in the blood shunt tube 10.
  • the peristaltic pump 13 provides power to transport the blood in the blood shunt tube 10 back into the blood vessel.
  • the use of the peristaltic pump 13 can control the blood flow, flow velocity and flow direction in the blood shunt tube 10 to prevent blood backflow. When this happens, the system is more stable.
  • the peristaltic pump 13 is optional. In some embodiments, the peristaltic pump 13 may not be used, and the blood pressure in the blood vessel may also be used to enable the blood to flow in the blood shunt tube 10.
  • a flow control valve (not shown) may be provided on the blood shunt tube 10, and the flow rate of the blood in the blood shunt tube 10 can be precisely adjusted and controlled by the flow control valve.
  • a dark room (not shown) is further included, and the photodetector 11 is disposed in the dark room to reduce interference from external ambient light.
  • the dark room can screen the ambient light outside, avoiding the external ambient light from causing interference to the photodetector 11, and ensuring higher detection accuracy of the photodetector 11.
  • the data acquisition device 12 may be installed in the dark room to form a whole with the photodetector 11, of course, it may also be installed separately in the dark room.
  • the data collector can be connected to the photodetector 11 through a standard digital interface, such as a USB interface or a serial port.
  • the processing device 200 may be a processor (such as an MCU or a CPU), which may be integrated into the data acquisition device 12.
  • the processing device 200 may also be a host computer, and the host computer communicates with the data acquisition device 12.
  • a display device 300 is further included.
  • the display device 300 is connected to the data acquisition device 12. In this way, the display device 300 can display information such as detection results.
  • the electric signal is a pulse signal
  • the data acquisition device 12 is specifically configured to periodically collect the number of the pulse signals output by the photodetector 11 per unit time according to the acquisition time interval
  • the processing device 200 is specifically It is used for calculating the fluorescence intensity according to the number of the pulse signals, and determining the content of the indole cyanine green according to the fluorescence intensity.
  • the collection time interval is 0.1us to 200min.
  • the photodetector 11 outputs pulse signals, and the data acquisition device 12 collects these pulse signals, and then the processing device 200 calculates the fluorescence intensity according to the number of pulse signals. The stronger the fluorescence intensity, the photodetector 11 unit time The greater the number of output pulses, finally, the processing device 200 determines the content of indole cyanine green by the intensity of the fluorescence. In this way, the content of indole cyanide green can be accurately detected.
  • the processing device 200 is further configured to generate indole cyan green data information according to the fluorescence intensity and send it to the display device 300 for display.
  • the indole cyan green data information is about "fluorescence intensity and measurement Time "relationship curve. In this way, the display device 300 can intuitively know the change process of the content of indocyanine green with time.

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  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un dispositif et un système de détection de vert d'indocyanine dans le sang, comprenant un tube de dérivation du sang, un détecteur photoélectrique et un dispositif d'acquisition de données. Le tube de dérivation de sang est constitué d'un matériau transparent et est relié à un organisme à tester, de telle sorte que le sang dans les vaisseaux sanguins de l'organisme à tester est dévié via le tube de dérivation de sang. Le détecteur photoélectrique est disposé à une distance prédéterminée du tube de dérivation du sang et est utilisé pour recevoir la fluorescence émise par le vert d'indocyanine dans le sang et convertir la fluorescence en un signal électrique. Le dispositif d'acquisition de données est connecté au détecteur photoélectrique et est utilisé pour acquérir le signal électrique.
PCT/CN2018/109558 2018-09-21 2018-10-10 Dispositif et système de détection de vert d'indocyanine dans le sang Ceased WO2020056808A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201811107298.0 2018-09-21
CN201821554495.2U CN209416918U (zh) 2018-09-21 2018-09-21 血液中吲哚氰绿的检测装置及系统
CN201811107298.0A CN109100342A (zh) 2018-09-21 2018-09-21 血液中吲哚氰绿的检测装置及系统
CN201821554495.2 2018-09-21

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WO2020056808A1 true WO2020056808A1 (fr) 2020-03-26

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102933140A (zh) * 2010-04-06 2013-02-13 卡迪奥克斯公司 用于循环系统畸形的改进的血液动力学检测的系统
CN103533972A (zh) * 2011-04-11 2014-01-22 弗雷森纽斯医疗护理德国有限责任公司 用于监测患者的治疗优选地用于监测血液透析、血液透滤和/或腹膜透析的方法和设备
CN104363820A (zh) * 2012-06-15 2015-02-18 弗雷森纽斯医疗护理德国有限责任公司 用于监控患者的体外血液处理的方法和装置

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102933140A (zh) * 2010-04-06 2013-02-13 卡迪奥克斯公司 用于循环系统畸形的改进的血液动力学检测的系统
CN103533972A (zh) * 2011-04-11 2014-01-22 弗雷森纽斯医疗护理德国有限责任公司 用于监测患者的治疗优选地用于监测血液透析、血液透滤和/或腹膜透析的方法和设备
CN104363820A (zh) * 2012-06-15 2015-02-18 弗雷森纽斯医疗护理德国有限责任公司 用于监控患者的体外血液处理的方法和装置

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