WO2020062001A1 - Procédé de détection de gène et système de détection de gène - Google Patents

Procédé de détection de gène et système de détection de gène Download PDF

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Publication number
WO2020062001A1
WO2020062001A1 PCT/CN2018/108252 CN2018108252W WO2020062001A1 WO 2020062001 A1 WO2020062001 A1 WO 2020062001A1 CN 2018108252 W CN2018108252 W CN 2018108252W WO 2020062001 A1 WO2020062001 A1 WO 2020062001A1
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WO
WIPO (PCT)
Prior art keywords
gene
data
detected
detection
gold standard
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2018/108252
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English (en)
Chinese (zh)
Inventor
辛泉预
杨政
贺立文
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen Bainuo International Life Science And Technology Co Ltd
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Shenzhen Bainuo International Life Science And Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by Shenzhen Bainuo International Life Science And Technology Co Ltd filed Critical Shenzhen Bainuo International Life Science And Technology Co Ltd
Priority to PCT/CN2018/108252 priority Critical patent/WO2020062001A1/fr
Publication of WO2020062001A1 publication Critical patent/WO2020062001A1/fr
Anticipated expiration legal-status Critical
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations

Definitions

  • the invention belongs to the field of gene detection, and particularly relates to a gene detection method and a gene detection system.
  • a gene is the basic unit of heredity. DNA or RNA sequences that carry hereditary information are copied to transfer the hereditary information to the next generation, direct the synthesis of proteins to express hereditary information, and thus control the expression of traits of individual organisms.
  • Gene detection is a technique for detecting DNA through blood, other body fluids, or cells. It takes the peripheral venous blood or other tissue cells of the subject, amplifies the gene information, and then uses specific equipment to detect the DNA molecules in the cells.
  • the technical problem to be solved by the present invention is to provide a gene detection method and a gene detection system, which aim to solve the problem that the current technology cannot effectively determine whether the detection result is correct when performing a genetic test.
  • the present invention is implemented as such, and a method for gene detection includes:
  • the gene data to be detected and the gold standard gene data are sent to a gene detection program at the same time, so that the gene detection program performs gene detection on the gene to be detected and the gold standard gene data to obtain the gene to be detected
  • the test results and the test results of the gold standard genetic data are sent to a gene detection program at the same time, so that the gene detection program performs gene detection on the gene to be detected and the gold standard gene data to obtain the gene to be detected
  • the step of simultaneously sending the gene data to be detected and the gold standard gene data to the gene detection program includes:
  • step of packaging the gene data to be detected with the gold standard gene data to obtain the data package to be detected includes:
  • the gene detection program performing the gene detection on the gene data to be detected and the gold standard gene data includes:
  • the gene detection program decrypts the data packet to be detected according to a decryption key to obtain the initial data packet
  • comparing the detection result of the gold standard gene data with a preset standard detection result includes:
  • a comparison result is obtained by comparing the detection result of the gold standard gene data with the standard detection result obtained by the search.
  • step of determining whether the comparison result is valid includes:
  • the gold standard genetic data includes at least one genetic test result data that is subjected to genetic test verification in advance.
  • An embodiment of the present invention further provides a gene detection system, including:
  • a detection unit configured to simultaneously send the gene data to be detected and the gold standard gene data to a gene detection program, so that the gene detection program performs gene detection on the gene to be detected and the gold standard gene data, respectively, to obtain the Describe the detection results of the genetic data to be detected and the detection results of the gold standard genetic data;
  • a comparison unit configured to compare the detection result of the gold standard genetic data with a preset standard detection result, and if the comparison result is determined to be valid, determine that the gene detection program detects the genetic data to be detected The result is correct. If the comparison result is determined to be invalid, it is determined that the detection result of the genetic data to be detected is incorrect.
  • the detection unit is specifically configured to:
  • the detection unit is further configured to:
  • Encrypt the initial data packet and send the data packet to be detected to the gene detection program, so that the gene detection program decrypts the data packet to be detected according to a decryption key to obtain the initial data packet, and Decompress the initial data packet to obtain the gene data to be detected and the gold standard gene data, and perform gene detection according to the gene data to be detected and the gold standard gene data.
  • comparison unit is further configured to:
  • the present invention has the beneficial effect that, when performing gene detection, the embodiment of the present invention sends the gene data to be detected and the gold standard gene data to the gene detection program, so that the gene detection program performs the gene data to be detected.
  • the gold standard gene data is also genetically tested to obtain the test result of the gold standard gene data and the gold standard gene data.
  • the gold standard gene data detection result is compared with a preset standard test result. Yes, if the obtained comparison result is determined to be valid, it is determined that the detection result of the gene data to be detected is correct, and if it is not valid, it is determined that the detection result of the gene data to be detected is incorrect.
  • the gold standard gene data that has been tested and verified in advance is also detected, and whether the result of the gene data to be tested is correct is determined according to the obtained gold standard gene data detection result.
  • the present invention can determine whether the detection result of the genetic data to be detected is correct based on the detection result of the gold standard genetic data. At the same time, because the gold standard genetic data is involved in the detection of each item, it can know which test item is the specific one. When problems occur, the incidence of errors is effectively avoided.
  • FIG. 1 is a flowchart of a gene detection method according to an embodiment of the present invention
  • FIG. 2 is a schematic structural diagram of a gene detection system according to an embodiment of the present invention.
  • FIG. 1 illustrates a gene detection method provided by an embodiment of the present invention, including:
  • step S101 the gene detection system sends the gene data to be detected and the gold standard gene data to the gene detection program at the same time.
  • the gene detection program After receiving the gene data to be detected and the gold standard gene data, the gene detection program will follow normal genes.
  • the detection process detects the gene data to be detected and the gold standard gene data, and obtains a detection result of the gene to be detected and a detection result of the gold standard gene data.
  • the gene detection program has the ability to simultaneously process two or more sets of data according to a normal detection process.
  • the genetic test is performed on both the gene data to be detected and the gold standard gene data as a detection standard, when determining whether the detection result of the gene data to be detected is correct, only the gold standard gene needs to be judged. Whether the test result of the data is consistent with the standard test result of the test verification in advance can determine whether the test result of the gene data to be tested is correct. Through this embodiment, the accuracy of the test result can be quickly known. If the test result is incorrect, it can be determined according to the test results of the gold standard genetic data at which step an error occurred, or which test item has a parameter change, etc. ,Convenient.
  • step S101 the genetic data to be detected and the gold standard gene data are detected in a multi-task and concurrent manner after entering the gene detection program, and do not interfere with each other. Both use the common gene detection in the gene detection process. Program and configuration parameters.
  • the gene detection program can perform gene detection on the data packet.
  • step S101 the gene data to be detected and the gold standard gene data are packaged to obtain a data packet to be detected, and the data packet to be detected is sent to Gene detection program.
  • the gene detection program will decompress the data packet to be tested to obtain the data to be tested and the gold standard gene data. More specifically, in order to ensure the integrity of the data and Confidentiality.
  • the packaged initial data package is encrypted to obtain the data package to be tested.
  • the specific encryption method can be set in advance. The key is stored in the genetic testing program in advance.
  • the genetic testing program After receiving the encrypted to-be-tested data packet, the genetic testing program will decrypt the to-be-tested data packet according to the decryption key to obtain the initial data packet, and then continue to the initial data packet.
  • the data package is decompressed to obtain the genetic data to be detected and the gold standard genetic data for genetic testing. More specifically, before the genetic detection system encrypts the initial data packet, a verification code is added, and the verification code and the initial data packet are encrypted to obtain a data packet to be detected.
  • the verification code is valid for a single time, that is, the data packet to be detected is decrypted to obtain the initial data packet and the verification code. If the verification code is read, the verification code is immediately invalidated.
  • the genetic testing program decrypts the data packet to be tested, and verifies the decoded verification code. If the verification code is invalid or wrong, the genetic testing program immediately stops the genetic test, and at the same time, it sends a prompt message to remind the current gene to be tested. data error.
  • the data to be tested and the gold standard gene data are packaged and encrypted, which can implement the entire process of genetic testing, that is, the package is encrypted only once from beginning to end, and the gene detection program is also performed only once. Decryption and verification code judgment can also be packed and encrypted during each project.
  • the package encryption is performed, which can well prevent the non-program change or the detection error of the genetic data to be detected due to the attack or tampering.
  • step S102 adopts a list comparison method, that is, using the gold standard gene data to perform genetic testing under standard conditions and standard conditions in advance, and saving the obtained data in the form of a list, and performing specific gene testing
  • the detection result of the gold standard genetic data can be compared with the result in the list. If the comparison result is valid, it is determined that the test results have not occurred in the test conditions or the test equipment has not changed.
  • one or more gold standard gene data may be set, a single gold standard gene data may run through the entire detection process, or different gold standard gene data may be used for different projects.
  • the test results of the gold standard genetic data include one or more. If the gene test only performs a certain aspect of the test, the test result of the gold standard genetic data is only one. If the gene test includes several items, the gold test The test results of standard genetic data include several results, which represent the results of each project and each process. By comparing each result, you can clearly understand which link or item has changed during the genetic testing process. Specifically, a method for judging whether the comparison result is valid may set a range of similarity in advance. In specific applications, it is determined whether the similarity between the detection result of the gold standard genetic data and the standard detection result is within a preset similarity range.
  • the comparison result is valid; if not, the comparison result is determined to be invalid. More specifically, because in the actual detection process, the detection results of the gold standard genetic data may not be completely consistent. When the similarity between the detection results of the gold standard genetic data and the standard detection results reaches a preset percentage, the gold standard is considered this time. The test results of the genetic data are correct.
  • the gene data A to be tested and the gold standard gene data B are sent to the gene testing program.
  • the gene testing program completes the testing of the gene data A and gold standard B according to the detection process.
  • the embodiment of the present invention also provides another way to detect.
  • step S101 insert the gold standard gene data into the genetic data to be detected to obtain a single pre-processed gene, and send the pre-processed gene to the gene detection program, so that the gene detection program performs gene detection on the pre-processed gene.
  • a test result which includes the test result of the gene data to be detected and the test result of the gold standard gene data.
  • the gold standard gene data is inserted into the gene data to be tested, and the head or tail of the gene data to be tested is generally selected to be inserted, so as not to affect the composition of the gene data to be tested and subsequent detection results.
  • the gene detection program performs gene detection on the to-be-detected gene data inserted with gold standard gene data, a single test result of the to-be-detected gene data + gold standard gene data will be obtained.
  • the gene detection system will extract the test results corresponding to the gold standard gene data in the test result, and compare the test results of the gold standard gene data with the standard test results that have been tested and verified in advance, if they are consistent. It means that the test result of the genetic data to be tested is correct.
  • the gene data A to be detected needs to be genetically tested.
  • the gold standard gene data B is inserted into the tail of the gene data A to be detected to obtain a preprocessed gene C.
  • the data format of the preprocessed gene is A + B.
  • Send to the gene detection program which detects the pre-processed gene C to obtain the test result c, extract the test result b corresponding to the gold standard gene data B in the test result, and compare the test result b with the gold standard gene
  • the standard test result b 'of data B is compared, and it is determined that all the values and expressions of the test result b are consistent with the standard test result b'. Then it is determined that the test result a of the genetic data A to be tested is correct, and the test result can be used. a Carry out subsequent processes.
  • FIG. 2 shows a gene detection system provided by an embodiment of the present invention, including:
  • the detecting unit 201 is configured to simultaneously send the gene data to be detected and the gold standard gene data to a gene detection program, so that the gene detection program performs gene detection on the gene to be detected and the gold standard gene data respectively, and A detection result of the gene data to be detected and a detection result of the gold standard gene data;
  • a comparison unit 202 is configured to compare the detection result of the gold standard gene data with a preset standard detection result, and if the comparison result is determined to be valid, determine that the gene detection program has performed on the genetic data to be detected. The detection result is correct. If the comparison result is determined to be invalid, it is determined that the detection result of the genetic data to be detected is incorrect.
  • the detecting unit 201 is specifically configured to:
  • the detecting unit 201 is further configured to:
  • Encrypt the initial data packet and send the data packet to be detected to the gene detection program, so that the gene detection program decrypts the data packet to be detected according to a decryption key to obtain the initial data packet, and Decompress the initial data packet to obtain the gene data to be detected and the gold standard gene data, and perform gene detection according to the gene data to be detected and the gold standard gene data.
  • comparison unit 202 is further configured to:
  • the disclosed methods and devices may be implemented in other ways.
  • the device embodiments described above are only schematic.
  • the division of the modules is only a logical function division.
  • multiple modules or components may be combined or It can be integrated into another device, or some features can be ignored or not implemented.
  • the displayed or discussed mutual coupling or direct coupling or communication connection may be through some interfaces, the indirect coupling or communication connection of the device or module, and may be electrical, mechanical or other forms.
  • the modules described as separate components may or may not be physically separated, and the components displayed as modules may or may not be physical modules, may be located in one place, or may be distributed on multiple network modules. Some or all of the modules may be selected according to actual needs to achieve the objective of the solution of this embodiment.
  • each functional module in each embodiment of the present invention may be integrated into one processing module, or each module may exist separately physically, or two or more modules may be integrated into one module.
  • the above integrated modules may be implemented in the form of hardware or software functional modules.
  • the integrated module When the integrated module is implemented in the form of a software functional module and sold or used as an independent product, it can be stored in a computer-readable storage medium.
  • the technical solution of the present invention essentially or part that contributes to the existing technology or all or part of the technical solution can be embodied in the form of a software product, which is stored in a storage medium , Including a number of instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the method described in each embodiment of the present invention.
  • the aforementioned storage medium includes: a U disk, a mobile hard disk, and a read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), magnetic disks or optical discs, and other media that can store program code.

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  • Life Sciences & Earth Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de détection de gène, comprenant les étapes consistant à : transmettre des données de gène à détecter et des données de gène standard d'or simultanément à un programme de détection de gène de telle sorte que le programme de détection de gène effectue une détection de gène sur les données de gène à détecter et les données de gène standard d'or respectivement pour obtenir un résultat de détection des données de gène à détecter et un résultat de détection des données de gène standard d'or ; comparer le résultat de détection des données de gène standard d'or avec un résultat de détection standard prédéfini ; si le résultat de comparaison est déterminé comme étant valide, déterminer que le programme de détection de gène a produit un résultat de détection correct par rapport aux données de gène à détecter ; et si le résultat de la comparaison est déterminé comme étant invalide, déterminer que le résultat des données de gène à détecter est incorrect.
PCT/CN2018/108252 2018-09-28 2018-09-28 Procédé de détection de gène et système de détection de gène Ceased WO2020062001A1 (fr)

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PCT/CN2018/108252 WO2020062001A1 (fr) 2018-09-28 2018-09-28 Procédé de détection de gène et système de détection de gène

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104520442A (zh) * 2012-05-22 2015-04-15 约翰霍普金斯大学 定量多重甲基化特异性PCR方法-cMethDNA、试剂及其用途
CN107066836A (zh) * 2017-06-15 2017-08-18 上海思路迪生物医学科技有限公司 基因检测管理方法及系统

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104520442A (zh) * 2012-05-22 2015-04-15 约翰霍普金斯大学 定量多重甲基化特异性PCR方法-cMethDNA、试剂及其用途
CN107066836A (zh) * 2017-06-15 2017-08-18 上海思路迪生物医学科技有限公司 基因检测管理方法及系统

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DUAN, MIN ET AL.: "Quality control and approval methods for genetic testing laboratories", CHINESE JOURNAL OF CLINICAL LABORATORY SCIENCE, vol. 36, no. 2, 28 February 2018 (2018-02-28), pages 135 - 138 *
WANG, YANFEI ET AL.: "Using the Standard Internal Quality Control Material for the Results of Evaluation", ADVANCED MEASUREMENT AND LABORATORY MANAGEMENT, no. 1, 31 December 2011 (2011-12-31), pages 30 - 31 *
WANG, ZHENXING: "Research on the Design of Laboratory Information Management System Based on Gene Detection and Comparison", WANFANG DATA KNOWLEDGE SERVICE PLATFORM, 20 May 2015 (2015-05-20), pages entire document *
XU, DUJUAN: ": Gene Testing Project in Hospital", TEXTBOOK FOR 13TH YEAR PLAN OF PHARMACY MAJOR IN GENERAL MEDICAL COLLEGES AND UNIVERSITIES OF CHINA, PHARMACEUTICAL CARE PRACTICE, 31 January 2016 (2016-01-31), pages 169 *

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