WO2020177059A1 - Système et procédé de détection de métaux lourds et de bactéries dans un analyte - Google Patents

Système et procédé de détection de métaux lourds et de bactéries dans un analyte Download PDF

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Publication number
WO2020177059A1
WO2020177059A1 PCT/CN2019/076886 CN2019076886W WO2020177059A1 WO 2020177059 A1 WO2020177059 A1 WO 2020177059A1 CN 2019076886 W CN2019076886 W CN 2019076886W WO 2020177059 A1 WO2020177059 A1 WO 2020177059A1
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WIPO (PCT)
Prior art keywords
kit according
strip
lateral flow
bacteria
conjugated
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Ceased
Application number
PCT/CN2019/076886
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English (en)
Inventor
Ho Wang Tong
Yu Hang LEUNG
Yu-Jen CHIANG
Wing Sze Chan
Yinghua Zhang
Connie Sau Kuen Kwok
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Nano and Advanced Materials Institute Ltd
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Nano and Advanced Materials Institute Ltd
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Priority to PCT/CN2019/076886 priority Critical patent/WO2020177059A1/fr
Publication of WO2020177059A1 publication Critical patent/WO2020177059A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

Definitions

  • the lateral flow strip of the present system further comprises at least a detection pad and an absorbent pad.
  • Each of the DNAzymes used in the present invention comprises at least one substrate strand and one enzyme strand which are specific for detecting one or more heavy metals.
  • they are partially or fully complementary to each other in terms of the nucleotide sequence thereof, and they bind in a way that a central catalytic core or domain is flanked on the enzyme strand and a single ribonucleotide is resulted on the substrate strand such that the substrate strand is cleavable at the ribonucleotide site when the DNAzyme is exposed to the respective heavy metal ions.
  • the lateral flow strip of the present invention further comprises at least one test line and one control line.
  • the signal amplifier comprises tyramide, horseradish peroxidase (HRP) and hydrogen peroxide.
  • FIG. 9A depicts a lateral flow strip for heavy metal detection
  • FIG. 9B shows a design of lateral flow strip for bacteria detection
  • the present system includes a lateral flow strip as a test platform which is configured to carry the essential chemical reagents for labeling and detecting the corresponding health-threatening contaminant (s) in water.
  • the lateral flow strip can include at least a detection pad and absorbent pad.
  • the DNAzyme specific for lead detection comprises an enzyme strand with a nucleotide sequence of 5’ -ACA GAC ATC ATC TCT GAA GTA GCG CCG CCG TAT AGT GAG-3’ (SEQ ID NO: 1) , and a substrate strand with a nucleotide sequence of 5’ -CTC ACT AT rA GGA AGA GAT GAT GTC TGT-3’ (SEQ ID NO: 4) , wherein a biotin-TEG is conjugated at the 5’ end of the nucleotide sequence of the substrate strand which can be represented by 5’ -/5BiotinTEG/-CTC ACT AT rA GGA AGA GAT GAT GTC TGT-3’ .

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Nanotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un kit de détection de métaux lourds et de bactéries dans un analyte. Le kit comprend au moins une bandelette de test à écoulement latéral de métal lourd comprenant une molécule de détection de désoxyribozyme. La molécule de détection de désoxyribozyme comprend un brin d'enzyme possédant un noyau catalytique central permettant une liaison à un métal lourd, et un brin de substrat, le brin de substrat étant conjugué à une biomolécule qui se lie à un indicateur fluorescent de point quantique conjugué à une biomolécule complémentaire. La bandelette de test à écoulement latéral comprend une ligne de test possédant une séquence complémentaire permettant de se lier au brin de substrat afin d'indiquer qu'un métal lourd est présent dans l'analyte ; il comprend également une ligne de contrôle permettant d'indiquer qu'un test a été correctement effectué. Le kit comprend en outre une bandelette de test de bactéries comprenant un anticorps qui se lie à au moins une bactérie dans l'analyte.
PCT/CN2019/076886 2019-03-04 2019-03-04 Système et procédé de détection de métaux lourds et de bactéries dans un analyte Ceased WO2020177059A1 (fr)

Priority Applications (1)

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PCT/CN2019/076886 WO2020177059A1 (fr) 2019-03-04 2019-03-04 Système et procédé de détection de métaux lourds et de bactéries dans un analyte

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PCT/CN2019/076886 WO2020177059A1 (fr) 2019-03-04 2019-03-04 Système et procédé de détection de métaux lourds et de bactéries dans un analyte

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113341149A (zh) * 2021-06-09 2021-09-03 军事科学院军事医学研究院环境医学与作业医学研究所 一种用于检测氟喹诺酮类抗生素的试剂盒和检测方法
CN114047166A (zh) * 2021-10-14 2022-02-15 上海市农产品质量安全中心 一种基于dna水凝胶的重金属离子快速检测方法

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JP2003052373A (ja) * 2001-08-10 2003-02-25 Nippon Laser & Electronics Lab カルシウムイオン依存性短鎖デオキシリボザイムを用いたセンサーチップ及び塩基配列検出方法
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US20120315621A1 (en) * 2010-05-26 2012-12-13 The Board Of Trustees Of The University Of Illinois Personal glucose meters for detection and quantification of a broad range of analytes
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WO2015164957A1 (fr) * 2014-04-28 2015-11-05 Juewen Liu Complexes phosphorothioate-dnazyme et leur utilisation
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CN107860765A (zh) * 2017-11-06 2018-03-30 首都医科大学 一种金属离子检测用探针、试剂盒、制备方法、应用
WO2018187829A1 (fr) * 2017-04-11 2018-10-18 Speedx Pty Ltd Cascades de détection

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US20120315621A1 (en) * 2010-05-26 2012-12-13 The Board Of Trustees Of The University Of Illinois Personal glucose meters for detection and quantification of a broad range of analytes
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CN105259314A (zh) * 2015-10-26 2016-01-20 广东省生态环境与土壤研究所 一种铅离子可视化检测方法及检测试剂盒
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WO2018187829A1 (fr) * 2017-04-11 2018-10-18 Speedx Pty Ltd Cascades de détection
CN107860765A (zh) * 2017-11-06 2018-03-30 首都医科大学 一种金属离子检测用探针、试剂盒、制备方法、应用

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113341149A (zh) * 2021-06-09 2021-09-03 军事科学院军事医学研究院环境医学与作业医学研究所 一种用于检测氟喹诺酮类抗生素的试剂盒和检测方法
CN113341149B (zh) * 2021-06-09 2023-08-22 军事科学院军事医学研究院环境医学与作业医学研究所 一种用于检测氟喹诺酮类抗生素的试剂盒和检测方法
CN114047166A (zh) * 2021-10-14 2022-02-15 上海市农产品质量安全中心 一种基于dna水凝胶的重金属离子快速检测方法

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