WO2020198264A8 - Clivages modifiés, utilisations et kits correspondants - Google Patents

Clivages modifiés, utilisations et kits correspondants Download PDF

Info

Publication number
WO2020198264A8
WO2020198264A8 PCT/US2020/024521 US2020024521W WO2020198264A8 WO 2020198264 A8 WO2020198264 A8 WO 2020198264A8 US 2020024521 W US2020024521 W US 2020024521W WO 2020198264 A8 WO2020198264 A8 WO 2020198264A8
Authority
WO
WIPO (PCT)
Prior art keywords
modified
cleavases
kits
related kits
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2020/024521
Other languages
English (en)
Other versions
WO2020198264A1 (fr
Inventor
Kevin Desai
Kevin L. GUNDERSON
Robert C. James
Lei Shi
Stephen VERESPY, III
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Encodia Inc
Original Assignee
Encodia Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU2020247918A priority Critical patent/AU2020247918B2/en
Priority to KR1020217033837A priority patent/KR102567902B1/ko
Priority to MX2021011726A priority patent/MX2021011726A/es
Priority to CA3134776A priority patent/CA3134776C/fr
Priority to EP20779890.1A priority patent/EP3947667A4/fr
Priority to CN202080023620.0A priority patent/CN113993997A/zh
Priority to JP2021557332A priority patent/JP2022526939A/ja
Application filed by Encodia Inc filed Critical Encodia Inc
Priority to SG11202110373WA priority patent/SG11202110373WA/en
Publication of WO2020198264A1 publication Critical patent/WO2020198264A1/fr
Publication of WO2020198264A8 publication Critical patent/WO2020198264A8/fr
Priority to US17/213,169 priority patent/US11427814B2/en
Anticipated expiration legal-status Critical
Priority to US17/850,516 priority patent/US11788080B2/en
Priority to US18/098,647 priority patent/US20230257727A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485—Exopeptidases (3.4.11-3.4.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/14—Dipeptidyl-peptidases and tripeptidyl-peptidases (3.4.14)
    • C12Y304/14004—Dipeptidyl-peptidase III (3.4.14.4)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des compositions et procédés d'inhibition, de traitement et/ou de prévention de la myélopathie dégénérative (DM) ou de la sclérose latérale amyotrophique (SLA). Selon la présente invention, des molécules d'acide nucléique pour inhiber l'expression du gène de superoxyde dismutase 1 (SOD) sont fournies. Dans un mode de réalisation particulier, les molécules d'acide nucléique comprennent un domaine de recuit lié de manière fonctionnelle à au moins un domaine effecteur, le domaine de recuit s'hybridant au pré-ARNm de SOD (par exemple, canin ou humain) et le domaine effecteur s'hybridant au snARN U1 du snRNP U1.
PCT/US2020/024521 2019-03-26 2020-03-24 Clivages modifiés, utilisations et kits correspondants Ceased WO2020198264A1 (fr)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CN202080023620.0A CN113993997A (zh) 2019-03-26 2020-03-24 修饰的切割酶，及其使用和相关试剂盒
KR1020217033837A KR102567902B1 (ko) 2019-03-26 2020-03-24 변형된 클리바아제, 이의 용도 및 관련 키트
MX2021011726A MX2021011726A (es) 2019-03-26 2020-03-24 Escindasas modificadas, usos de las mismas y kits relacionados.
CA3134776A CA3134776C (fr) 2019-03-26 2020-03-24 Clivages modifies, utilisations et kits correspondants
EP20779890.1A EP3947667A4 (fr) 2019-03-26 2020-03-24 Clivages modifiés, utilisations et kits correspondants
JP2021557332A JP2022526939A (ja) 2019-03-26 2020-03-24 修飾された切断酵素、その使用、および関連キット
SG11202110373WA SG11202110373WA (en) 2019-03-26 2020-03-24 Modified cleavases, uses thereof and related kits
AU2020247918A AU2020247918B2 (en) 2019-03-26 2020-03-24 Modified cleavases, uses thereof and related kits
US17/213,169 US11427814B2 (en) 2019-03-26 2021-03-25 Modified cleavases, uses thereof and related kits
US17/850,516 US11788080B2 (en) 2019-03-26 2022-06-27 Modified cleavases, uses thereof and related kits
US18/098,647 US20230257727A1 (en) 2019-03-26 2023-01-18 Modified cleavases, uses thereof and related kits

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201962824157P 2019-03-26 2019-03-26
US201962823927P 2019-03-26 2019-03-26
US62/823,927 2019-03-26
US62/824,157 2019-03-26
US201962931737P 2019-11-06 2019-11-06
US62/931,737 2019-11-06

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
US18/016,665 Continuation-In-Part US20240011009A1 (en) 2020-03-24 2021-03-19 Modified dipeptide cleavases, uses thereof and related kits
PCT/US2021/023347 Continuation-In-Part WO2021194908A1 (fr) 2019-03-26 2021-03-19 Clivases dipeptidiques modifiées, utilisations correspondantes et kits correspondants

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US17/213,169 Continuation-In-Part US11427814B2 (en) 2019-03-26 2021-03-25 Modified cleavases, uses thereof and related kits

Publications (2)

Publication Number Publication Date
WO2020198264A1 WO2020198264A1 (fr) 2020-10-01
WO2020198264A8 true WO2020198264A8 (fr) 2021-01-07

Family

ID=72612078

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2020/024521 Ceased WO2020198264A1 (fr) 2019-03-26 2020-03-24 Clivages modifiés, utilisations et kits correspondants

Country Status (8)

Country Link
EP (1) EP3947667A4 (fr)
JP (1) JP2022526939A (fr)
KR (1) KR102567902B1 (fr)
CN (1) CN113993997A (fr)
AU (1) AU2020247918B2 (fr)
MX (1) MX2021011726A (fr)
SG (1) SG11202110373WA (fr)
WO (1) WO2020198264A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
US11427814B2 (en) 2019-03-26 2022-08-30 Encodia, Inc. Modified cleavases, uses thereof and related kits
WO2021194908A1 (fr) 2020-03-24 2021-09-30 Encodia, Inc. Clivases dipeptidiques modifiées, utilisations correspondantes et kits correspondants
EP3962930A4 (fr) * 2019-04-30 2024-03-27 Encodia, Inc. Procédés et réactifs pour le clivage de l'acide aminé n-terminal d'un polypeptide
WO2021141924A1 (fr) 2020-01-07 2021-07-15 Encodia, Inc. Procédés de formation d'un complexe stable et kits associés
WO2022040098A1 (fr) 2020-08-19 2022-02-24 Encodia, Inc. Procédés de codage séquentiel et kits associés
WO2022147334A1 (fr) * 2020-12-31 2022-07-07 Encodia, Inc. Métalloenzymes pour la reconnaissance biomoléculaire de peptides modifiés en position n-terminale
CN117881691A (zh) * 2021-08-02 2024-04-12 丝芭博株式会社 多孔质体及其制造方法
CN114460052B (zh) * 2022-01-11 2023-06-20 武汉理工大学 一种基于荧光碳量子点直接检测丙酮酸钠浓度的方法
CN116426509B (zh) * 2023-04-27 2024-07-30 湖北佶凯星生物科技有限公司 一种碱性蛋白酶组合突变体及其应用
WO2026030423A1 (fr) * 2024-07-31 2026-02-05 Glyphic Biotechnologies, Inc. Réactifs de séquençage utilisant des agents guanidinyants

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW257792B (fr) * 1992-10-01 1995-09-21 Lilly Co Eli
US6875851B1 (en) * 1999-03-05 2005-04-05 University Of Georgia Research Foundation, Inc. Prolyl tripeptidyl peptidases nucleic acid of Porphyromonas gingivalis
CZ20031301A3 (cs) * 2000-10-12 2003-10-15 Ferring Bv Izolovaná nukleová kyselina, polypeptid, expresní vektor a protilátka
EP1578935A2 (fr) * 2002-10-10 2005-09-28 Diversa Corporation Proteases, acides nucleiques les codant et leurs procedes de fabrication et d'utilisation
WO2005015239A2 (fr) * 2003-08-07 2005-02-17 Cornell Research Foundation, Inc. Procede pour marquer des proteines au terminal n
EP1972349A1 (fr) * 2007-03-21 2008-09-24 Biocompatibles UK Limited Peptides à fusion GLP-1 associée à un/plusieurs polymères, leur production et utilisation
EP1975176A1 (fr) * 2007-03-27 2008-10-01 Biocompatibles UK Limited Nouveaux peptides à fusion GLP-1, leur production et utilisation
EP2034020A1 (fr) * 2007-08-30 2009-03-11 Procomcure Biotech GmbH Procédé pour la fabrication d'un peptide modifié
WO2010065322A1 (fr) * 2008-12-01 2010-06-10 Research Triangle Institute Identification simultanée de multitudes de polypeptides
EP2694089B1 (fr) * 2011-04-05 2024-06-05 Cellectis Nouveaux échafaudages de protéine tale et leurs utilisations
BR112014017919A2 (pt) * 2012-01-26 2017-06-27 Novozymes As uso de um poliptídeo variante, composição, polinucleotídeo isolado, constructo de ácido nucleico ou vetor de expressão, célula hospedeira de expressão recombinante, métodos de produção do pilipeptídeo, para melhoria do valor nutricional de uma ração animal, e para o tratamento de proteínas, planta, parte de planta ou célula de planta transgênica, aditivo de ração animal, ração animal, e, composição de ração animal ou detergente
US9435810B2 (en) * 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
AU2017259794B2 (en) * 2016-05-02 2023-04-13 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding
US11427814B2 (en) * 2019-03-26 2022-08-30 Encodia, Inc. Modified cleavases, uses thereof and related kits
WO2021194908A1 (fr) * 2020-03-24 2021-09-30 Encodia, Inc. Clivases dipeptidiques modifiées, utilisations correspondantes et kits correspondants

Also Published As

Publication number Publication date
JP2022526939A (ja) 2022-05-27
WO2020198264A1 (fr) 2020-10-01
EP3947667A1 (fr) 2022-02-09
SG11202110373WA (en) 2021-10-28
KR102567902B1 (ko) 2023-08-18
CN113993997A (zh) 2022-01-28
KR20210143236A (ko) 2021-11-26
CA3134776A1 (fr) 2020-10-01
EP3947667A4 (fr) 2022-12-28
AU2020247918B2 (en) 2022-06-30
MX2021011726A (es) 2021-10-22
AU2020247918A1 (en) 2021-10-21

Similar Documents

Publication Publication Date Title
WO2020198264A8 (fr) Clivages modifiés, utilisations et kits correspondants
WO2020102741A8 (fr) Procédés et compositions de séquençage de protéines
NZ781143A (en) Anti-vegf protein compositions and methods for producing the same
WO2020214336A3 (fr) Chimie d'échange soufre-hétérocycle et utilisations associées
EP4467650A3 (fr) Protéines d'échafaudage
DE602008005885D1 (de) Aufreinigung von faktor viii unter verwendung eine
WO2021236716A3 (fr) Procédés, systèmes et kits pour le traitement et l'analyse de polypeptides
ATE495447T1 (de) Peptidantikörperdepletion und anwendung zur probenvorbereitung für massenspektrometrie
WO2005046709A3 (fr) Peptides et peptides supportes pour le traitement de troubles dermatologiques
MX2025012685A (es) Anticuerpos para claudin6 y metodos para tratar el cancer
MX2020010806A (es) Peptidos anti-inflamatorios y usos de los mismos.
MX2021012047A (es) Polipeptidos de fusion de serpina y metodos para utilizar los mismos.
WO2021236983A3 (fr) Procédés et compositions de séquençage de protéines
WO2021021964A3 (fr) Procédés de quantification de protéines de fusion de frataxine et de frataxine
IL172729A0 (en) Reagents and methods for the formation of disulfide bonds and the glycosylation of proteins
HK1249092A1 (zh) 用於样品制备尤其是用於质谱分析的方式和方法
PH12019502449A1 (en) Anti-jagged1 antigen binding proteins
WO2004076646A3 (fr) Methodes et compositions destinees a traiter le cancer du col uterin
EP4155734A3 (fr) Procede
WO2004043370A3 (fr) Procedes et compositions pour la modulation de la proteine activatrice 1
MX2021016092A (es) Metodos de purificacion de proteinas.
DE60308176D1 (de) Vorrichtung von peptid- oder proteindarstellung, verfahren zu deren herstellung und deren verwendung
WO2022241212A3 (fr) Peptides de mertk et leurs utilisations
DE69734779D1 (de) Erhöhte ausbeuten eines kristallinen proteins durch verwendung eines festen adsorptionsmaterials

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20779890

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 3134776

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2021557332

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 20217033837

Country of ref document: KR

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 2020247918

Country of ref document: AU

Date of ref document: 20200324

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 2020779890

Country of ref document: EP

Effective date: 20211026

WWW Wipo information: withdrawn in national office

Ref document number: 2020779890

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 11202110373W

Country of ref document: SG

WWP Wipo information: published in national office

Ref document number: 11202110373W

Country of ref document: SG