WO2021206573A1 - A method for processing raw plant material, especially legumes into protein having a nutritional and feed value, bioethanol, biogas and fertiliser materials - Google Patents

A method for processing raw plant material, especially legumes into protein having a nutritional and feed value, bioethanol, biogas and fertiliser materials Download PDF

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WO2021206573A1
WO2021206573A1 PCT/PL2021/050022 PL2021050022W WO2021206573A1 WO 2021206573 A1 WO2021206573 A1 WO 2021206573A1 PL 2021050022 W PL2021050022 W PL 2021050022W WO 2021206573 A1 WO2021206573 A1 WO 2021206573A1
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protein
fraction
fertiliser
subjected
solid
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French (fr)
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Tomasz Kapela
Krzysztof Makowski
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Biotechnika Tomasz Kapela
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Biotechnika Tomasz Kapela
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Priority to EP21784546.0A priority Critical patent/EP4132296A4/en
Priority to US17/917,932 priority patent/US20230133658A1/en
Publication of WO2021206573A1 publication Critical patent/WO2021206573A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12F—RECOVERY OF BY-PRODUCTS OF FERMENTED SOLUTIONS; DENATURED ALCOHOL; PREPARATION THEREOF
    • C12F3/00—Recovery of by-products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
    • A23J1/148—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by treatment involving enzymes or microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/023—Methane
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00—Preparation of other alcoholic beverages
    • C12G3/02—Preparation of other alcoholic beverages by fermentation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Definitions

  • the invention relates to a method for processing raw plant material, especially legumes into protein having a nutritional and feed value, bioethanol, biogas and fertiliser materials.
  • Document W02005079190 discloses a process for providing conversion of biomass materials with a high level of solids, in particular grains of cereals and legumes, in order to obtain a conversion with a high level of solids to produce ethanol and high protein by-products, such as dried stillage with solutes, comprising: treating biomass material with an acid, heating the acid-treated biomass material at a temperature sufficient for pasting and solubilisation of starch and breakdown of carbohydrates other than starch and lignin, adding stillage, cooling the suspension to temperatures which allow the use of liquefying enzymes, adding saccharification enzymes to hydrolyse the starch and non-starch carbohydrates into respective sugars, cooling the hydrolysate and transferring it to an ethanol fermentor, concentrating the ethanol and obtaining the fraction containing the biomass residue with an addition of with protein from the dried stillage with solubles.
  • Document ATA56991 discloses a method of producing ethanol from protein-rich starch-containing raw materials, e.g. from legumes, wherein the raw materials are milled, the starch content undergoes enzymatic degradation or acid hydrolysis to fer mentable sugars, and the sugars are converted into ethanol by way of fermentation, wherein the milled raw materials are converted into mash in a liquid receptacle, and proteins are washed away prior to starch degradation, followed by solid particles containing most of the starch being separated from the mash, and the protein content is precipitated from the washing liquid by raising the temperature to 50 to 90 degrees C, preferably 60 to 70 degrees C, and lowering the pH to 1.0 to 5.0, preferably 2.5 to 4.5.
  • the essence of the invention is a method for processing raw vegetable material, es pecially legumes, and in particular peas, field beans, lentils, soya beans, lupin beans, chickpeas, winged peas, Arachis, pigeon peas, jack beans, earth peas, lablab beans, peavines, winter peas, vetches, serradella, into protein having a nutritional and feed value, bioethanol, biogas and fertiliser materials, consisting in separating the raw material in the form of husks, milling seed residues, extracting the protein to a liquid phase, separating the solid phase and submitting it to enzymatic hydrolysis, microbial fermentation and biogasification, characterised in that the plant material undergoes a dehusking process, and the resulting husks are hydrolysed and biogasified using the CSTR method.
  • the dehusked raw material is milled to a fraction with a size of 0,1 to 3 mm and extracted with stirring for 1 to 12 h at a temperature of 20 ° to 80 °C, pH 6 to 10 in excess extraction buffer in the range of 3 to 15 times the plant material feed by weight.
  • the insoluble solid fraction is separated and subjected to enzymatic hydrolysis using liquefaction and saccharification enzymes, then subjected to ethanol fer mentation at a temperature of 25 ° to 40 °C for 24 to 72 h at a pH of 4 to 8, where the ethanol formed undergoes distillation, and the digestate is transferred as a substrate for biogas production.
  • the liquid fraction is precipitated for 1 to 24 h at a temperature of 0 to 30 °C at pH 2 to 6 into a solid in the form of a protein precipitate, which is washed with an extraction buffer with a pH of 4 to 8 in a mass ratio of solid fraction : buffer 1:2.
  • the liquid residue is then removed and subjected to a CSTR biogasification process, and the wet precipitate obtained during the biogasi fication is used as a fertiliser or is formed into dry pellets and used as an organic fertiliser.
  • the solid form in the form of a protein precipitate after the precipitation process is washed several times with an extraction buffer of pH 4 to 8 in a mass ratio of solid fraction: buffer 1:2.
  • the solid form in the form of a protein precipitate after the precipitation process is washed with an aqueous solution with an organic solvent and dried to obtain the dry fraction.
  • the solid form in the form of a protein precipitate after the precipitation process is washed with an aqueous solution with ethanol at a concentration of 30 to 90%, pH 4 to 8, at a mass ratio of solid fraction: solution of 1:2.
  • the solid form in the form of a protein precipitate after the precipitation process is washed with an aqueous solution with 30 to 90% isopropanol at pH 4 to 8 in a mass ratio of solid fraction: solution of 1:2.
  • the method according to the invention allows for processing raw plant material, es pecially legumes, into pure forms of protein having a nutritional and feed value while making use of the resulting by-product streams.
  • the method according to the invention is zero-waste, as it is carried out in a closed cycle, where the waste produced in the process is used to produce bioethanol, biogas and fertiliser materials.
  • the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification.
  • the raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR (Continuous Stirred Tank Reactor) method.
  • CSTR Continuous Stirred Tank Reactor
  • the CSTR method involves a continuous process of residue biogasification in a bioreactor to which there is added a medium in the form of waste and a consortium of anaerobic bacteria that transform the available carbon into methane (biogas).
  • the dehusked material is mechanically milled to a fraction with a size of 0,1 mm, suspended in ex traction buffer pH 9 at a feedstock to buffer ratio of 1:10 by weight and is extracted for 1 h at 40 °C with stirring.
  • the insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fer mentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production.
  • the pH is adjusted to 4.0, the temperature is lowered to 4 °C, and the precipitation process is carried out for 4 h.
  • the protein precipitate formed during the process is separated from the liquid phase and washed once with water pH 4 in a wet protein : water ratio of 1:2, washed sub sequently with a 30% ethanol solution pH 4 in a wet protein : water ratio of 1:2 and dried.
  • the liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
  • the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein, drying the protein and subjecting the residue to ethanol fermentation and biogasification.
  • the raw material in the form of legumes is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasification using the CSTR (Continuous Stirred Tank Reactor) method.
  • CSTR Continuous Stirred Tank Reactor
  • the CSTR method involves a continuous process of residue biogasification in a bioreactor to which there is added a medium in the form of waste and a consortium of anaerobic bacteria that transform the available carbon into methane (biogas).
  • the dehusked material is mechanically milled to a fraction with a size of 2 mm, suspended in extraction buffer pH 8 at a feedstock to buffer ratio of 1:10 by weight and is extracted for 1 h at 40 °C with stirring.
  • the insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production.
  • the pH is adjusted to 4.0, the temperature is lowered to 4 °C, and the precipitation process is carried out for 4 h.
  • the resulting protein precipitate is separated from the liquid phase and washed with water pH 7 in a wet protein : water ratio of 1:2 and dried.
  • the liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
  • the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification.
  • the raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method.
  • the dehusked material is mechanically milled to a fraction with a size of 0,5 mm, suspended in extraction buffer pH 8 at a feedstock to buffer ratio of 1:15 by weight and is extracted for 12 h at 20 °C with stirring.
  • the insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production.
  • the pH is adjusted to 5,0, the temperature is lowered to 10 °C and the precipitation process is conducted for 24 h, the protein precipitate formed during the process is separated from the liquid phase and washed twice with water pH 6 in a wet protein : water ratio of 1: 3, washed subsequently with a 10% isopropanol solution pH 7 in a wet protein : water ratio of 1 : 1 and dried.
  • the liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
  • the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification.
  • the raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method.
  • the dehusked material is mechanically milled to a fraction with a size of 3 mm, suspended in extraction buffer pH 6 at a feedstock to buffer ratio of 1: 4 by weight and is extracted for 1 h at 80 °C with stirring.
  • the insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production.
  • the pH is adjusted to 2.0, the temperature is lowered to 30 °C, and the precipitation process is carried out for 12 h.
  • the protein precipitate obtained is separated from the liquid phase and washed four times with water pH 5 in a wet protein : water ratio of 1: 5, washed subsequently with a 10% ethanol solution pH 7 in a wet protein : water ratio of 1:2 and dried.
  • the liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
  • the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification.
  • the raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method.
  • the dehusked material is mechanically milled to a fraction with a size of 0,2 mm, suspended in extraction buffer pH 7 at a feedstock to buffer ratio of 1:10 by weight and is extracted for 1 h at 40 °C with stirring.
  • the insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production.
  • the pH is adjusted to 5.0, the temperature is lowered to 10 °C, and the precipitation process is carried out for 24 h.
  • the resulting protein precipitate is separated from the liquid phase and washed twice with water pH 4 in a wet protein : water ratio of 1:2 and dried.
  • the liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
  • the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification.
  • the raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method.
  • the dehusked material is mechanically milled to a fraction with a size of 1 mm, suspended in extraction buffer pH 10 at a feedstock to buffer ratio of 1: 3 by weight and is extracted for 6 h at 50 °C with stirring.
  • the insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production.
  • the pH is adjusted to 2,0, the temperature is lowered to 0 °C and the precipitation process is conducted for 1 h, the protein precipitate formed during the process is separated from the liquid phase and washed once with water pH 8 in a wet protein : water ratio of 1: 5, washed subsequently with a 90% ethanol solution pH 4 in a wet protein : water ratio of 1 : 1 and dried.
  • the liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
  • the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification.
  • the raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method.
  • the dehusked material is mechanically milled to a fraction with a size of 0, 1 mm, suspended in extraction buffer pH 9 at a feedstock to buffer ratio of 1:10 by weight and is extracted for 2 h at 40 °C with stirring.
  • the insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production.
  • the pH is adjusted to 4.0, the temperature is lowered to 0 °C, and the precipitation process is carried out for 24 h.
  • the resulting protein precipitate is separated from the liquid phase and washed with a 40% ethanol solution pH 4 in a wet protein : solution mass ratio 1:2 and dried.
  • the liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
  • the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification.
  • the raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method.
  • the dehusked material is mechanically milled to a fraction with a size of 2 mm, suspended in extraction buffer pH 7 at a feedstock to buffer ratio of 1: 5 by weight and is extracted for 8 h at 20 °C with stirring.
  • the insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production.
  • the pH is adjusted to 2.0, the temperature is lowered to 10 °C, and the precipitation process is carried out for 12 h.
  • the protein precipitate obtained is separated from the liquid phase and washed three times with water pH 5 in a wet protein : water mass ratio of 1: 5, washed subsequently with a 90 % isopropanol solution pH 4 in a wet protein : water mass ratio of 1: 2 and dried.
  • the liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
  • the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification.
  • the raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method.
  • the dehusked material is mechanically milled to a fraction with a size of 0,1 mm, suspended in extraction buffer pH 10 at a feedstock to buffer ratio of 1: 15 by weight and is extracted for 1 h at 80 °C with stirring.
  • the insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production.
  • the pH is adjusted to 6.0, the temperature is lowered to 0 °C, and the precipitation process is carried out for 1 h.
  • the resulting protein precipitate is separated from the liquid phase and washed with a 60% isopropanol solution pH 8 in a wet protein : solution mass ratio 1:2 and dried.
  • the liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
  • the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification.
  • the raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method.
  • the dehusked material is mechanically milled to a fraction with a size of 2 mm, suspended in extraction buffer pH 8 at a feedstock to buffer ratio of 1: 8 by weight and is extracted for 2 h at 30 °C with stirring.
  • the insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production.
  • the pH is adjusted to 3.0, the temperature is lowered to 20 °C, and the precipitation process is carried out for 8 h.
  • the protein precipitate obtained is separated from the liquid phase and washed three times with water pH 5 in a wet protein : water mass ratio of 1: 3, washed subsequently with a 40% isopropanol solution pH 5 in a wet protein : water mass ratio of 1: 2 and dried.
  • the liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.

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Abstract

A method for processing raw plant material, especially legumes into protein having a nu-tritional and feed value, bioethanol, biogas and fertiliser materials characterised in that the rawplant material is subjected to a dehusking process, followed by the dehusked raw material beingcrushed and subjected to extraction with stirring, wherein the insoluble solid fraction is separatedand subjected to enzymatic hydrolysis using liquefying and saccharifying enzymes, thensubjected to ethanol fermentation, where the produced ethanol is distilled and the digestate istransferred as a substrate for biogas production, and the liquid fraction after the extractionprocess is subjected to a process of precipitation to a solid form in the form of protein precipitate,which is washed with extraction buffer, after which the liquid residue is removed and subjectedto a biogasification process, and the wet biogas residue is used as a fertiliser or formed into drypellets and used as an organic fertiliser.

Description

Title of Invention: A method for processing raw plant material, especially legumes into protein having a nutritional and feed value, bioethanol, biogas and fertiliser materials
[0001] The invention relates to a method for processing raw plant material, especially legumes into protein having a nutritional and feed value, bioethanol, biogas and fertiliser materials.
[0002] Legumes have been known and cultivated by mankind for thousands of years. Their popularity and share in the diet has varied many times over this period. In countries of Central and South America and Asia as well as in some of the Middle East, plants such as peas, beans, soya beans and chickpeas are a diet staple, while in other parts of the world there an increased consumption of legumes is observed in addition to that of soya beans, which continue to rank first in terms of crops. Due to their composition, rich in protein and carbohydrates, they are an excellent component of the diet. However, unfortunately, they also have some disadvantages, such as the presence of sugars that are not digested by humans and anti-nutrients. These disadvantages do not disqualify legumes as food, but they do involve certain limitations.
[0003] Consumption of legumes is not constant and production volumes depend partly on dietary trends. One of the main trends affecting the increased farming of legumes is the elimination or reduction of gluten in human diet. As it is, legumes are virtually the only alternative to this protein obtained from cereals. Due to growing knowledge and awareness of nutrition, apart from reducing gluten in the diet, people also disfavour excessive amounts of carbohydrates, which are a group of compounds that are abundant in seeds of legumes and can represent as much as more than 50% of the dry weight of the seeds. It is therefore a very interesting challenge to obtain pure forms of protein from legumes while making use of the resulting by-product streams.
[0004] Document W02005079190 discloses a process for providing conversion of biomass materials with a high level of solids, in particular grains of cereals and legumes, in order to obtain a conversion with a high level of solids to produce ethanol and high protein by-products, such as dried stillage with solutes, comprising: treating biomass material with an acid, heating the acid-treated biomass material at a temperature sufficient for pasting and solubilisation of starch and breakdown of carbohydrates other than starch and lignin, adding stillage, cooling the suspension to temperatures which allow the use of liquefying enzymes, adding saccharification enzymes to hydrolyse the starch and non-starch carbohydrates into respective sugars, cooling the hydrolysate and transferring it to an ethanol fermentor, concentrating the ethanol and obtaining the fraction containing the biomass residue with an addition of with protein from the dried stillage with solubles.
[0005] Document ATA56991 discloses a method of producing ethanol from protein-rich starch-containing raw materials, e.g. from legumes, wherein the raw materials are milled, the starch content undergoes enzymatic degradation or acid hydrolysis to fer mentable sugars, and the sugars are converted into ethanol by way of fermentation, wherein the milled raw materials are converted into mash in a liquid receptacle, and proteins are washed away prior to starch degradation, followed by solid particles containing most of the starch being separated from the mash, and the protein content is precipitated from the washing liquid by raising the temperature to 50 to 90 degrees C, preferably 60 to 70 degrees C, and lowering the pH to 1.0 to 5.0, preferably 2.5 to 4.5.
[0006] The essence of the invention is a method for processing raw vegetable material, es pecially legumes, and in particular peas, field beans, lentils, soya beans, lupin beans, chickpeas, winged peas, Arachis, pigeon peas, jack beans, earth peas, lablab beans, peavines, winter peas, vetches, serradella, into protein having a nutritional and feed value, bioethanol, biogas and fertiliser materials, consisting in separating the raw material in the form of husks, milling seed residues, extracting the protein to a liquid phase, separating the solid phase and submitting it to enzymatic hydrolysis, microbial fermentation and biogasification, characterised in that the plant material undergoes a dehusking process, and the resulting husks are hydrolysed and biogasified using the CSTR method. The dehusked raw material is milled to a fraction with a size of 0,1 to 3 mm and extracted with stirring for 1 to 12 h at a temperature of 20 ° to 80 °C, pH 6 to 10 in excess extraction buffer in the range of 3 to 15 times the plant material feed by weight. The insoluble solid fraction is separated and subjected to enzymatic hydrolysis using liquefaction and saccharification enzymes, then subjected to ethanol fer mentation at a temperature of 25 ° to 40 °C for 24 to 72 h at a pH of 4 to 8, where the ethanol formed undergoes distillation, and the digestate is transferred as a substrate for biogas production. After the extraction process, the liquid fraction is precipitated for 1 to 24 h at a temperature of 0 to 30 °C at pH 2 to 6 into a solid in the form of a protein precipitate, which is washed with an extraction buffer with a pH of 4 to 8 in a mass ratio of solid fraction : buffer 1:2. The liquid residue is then removed and subjected to a CSTR biogasification process, and the wet precipitate obtained during the biogasi fication is used as a fertiliser or is formed into dry pellets and used as an organic fertiliser.
[0007] Preferably, the solid form in the form of a protein precipitate after the precipitation process is washed several times with an extraction buffer of pH 4 to 8 in a mass ratio of solid fraction: buffer 1:2.
[0008] Preferably, the solid form in the form of a protein precipitate after the precipitation process is washed with an aqueous solution with an organic solvent and dried to obtain the dry fraction.
[0009] Preferably, the solid form in the form of a protein precipitate after the precipitation process is washed with an aqueous solution with ethanol at a concentration of 30 to 90%, pH 4 to 8, at a mass ratio of solid fraction: solution of 1:2.
[0010] Preferably, the solid form in the form of a protein precipitate after the precipitation process is washed with an aqueous solution with 30 to 90% isopropanol at pH 4 to 8 in a mass ratio of solid fraction: solution of 1:2.
[0011] The method according to the invention allows for processing raw plant material, es pecially legumes, into pure forms of protein having a nutritional and feed value while making use of the resulting by-product streams. The method according to the invention is zero-waste, as it is carried out in a closed cycle, where the waste produced in the process is used to produce bioethanol, biogas and fertiliser materials.
[0012] Embodiment I
[0013] In the embodiment, the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification. The raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR (Continuous Stirred Tank Reactor) method. The CSTR method involves a continuous process of residue biogasification in a bioreactor to which there is added a medium in the form of waste and a consortium of anaerobic bacteria that transform the available carbon into methane (biogas). The dehusked material is mechanically milled to a fraction with a size of 0,1 mm, suspended in ex traction buffer pH 9 at a feedstock to buffer ratio of 1:10 by weight and is extracted for 1 h at 40 °C with stirring. The insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fer mentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production. In the soluble fraction, the pH is adjusted to 4.0, the temperature is lowered to 4 °C, and the precipitation process is carried out for 4 h. The protein precipitate formed during the process is separated from the liquid phase and washed once with water pH 4 in a wet protein : water ratio of 1:2, washed sub sequently with a 30% ethanol solution pH 4 in a wet protein : water ratio of 1:2 and dried. The liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser. [0014] Embodiment II
[0015] In the embodiment, the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein, drying the protein and subjecting the residue to ethanol fermentation and biogasification. The raw material in the form of legumes is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasification using the CSTR (Continuous Stirred Tank Reactor) method. The CSTR method involves a continuous process of residue biogasification in a bioreactor to which there is added a medium in the form of waste and a consortium of anaerobic bacteria that transform the available carbon into methane (biogas). The dehusked material is mechanically milled to a fraction with a size of 2 mm, suspended in extraction buffer pH 8 at a feedstock to buffer ratio of 1:10 by weight and is extracted for 1 h at 40 °C with stirring. The insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production. In the soluble fraction, the pH is adjusted to 4.0, the temperature is lowered to 4 °C, and the precipitation process is carried out for 4 h. The resulting protein precipitate is separated from the liquid phase and washed with water pH 7 in a wet protein : water ratio of 1:2 and dried. The liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
[0016] Embodiment III
[0017] In the embodiment, the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification. The raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method. The dehusked material is mechanically milled to a fraction with a size of 0,5 mm, suspended in extraction buffer pH 8 at a feedstock to buffer ratio of 1:15 by weight and is extracted for 12 h at 20 °C with stirring. The insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production. In the soluble fraction, the pH is adjusted to 5,0, the temperature is lowered to 10 °C and the precipitation process is conducted for 24 h, the protein precipitate formed during the process is separated from the liquid phase and washed twice with water pH 6 in a wet protein : water ratio of 1: 3, washed subsequently with a 10% isopropanol solution pH 7 in a wet protein : water ratio of 1 : 1 and dried. The liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
[0018] Embodiment IV
[0019] In the embodiment, the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification. The raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method. The dehusked material is mechanically milled to a fraction with a size of 3 mm, suspended in extraction buffer pH 6 at a feedstock to buffer ratio of 1: 4 by weight and is extracted for 1 h at 80 °C with stirring. The insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production. In the soluble fraction, the pH is adjusted to 2.0, the temperature is lowered to 30 °C, and the precipitation process is carried out for 12 h. The protein precipitate obtained is separated from the liquid phase and washed four times with water pH 5 in a wet protein : water ratio of 1: 5, washed subsequently with a 10% ethanol solution pH 7 in a wet protein : water ratio of 1:2 and dried. The liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
[0020] Embodiment V
[0021] In the embodiment, the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification. The raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method. The dehusked material is mechanically milled to a fraction with a size of 0,2 mm, suspended in extraction buffer pH 7 at a feedstock to buffer ratio of 1:10 by weight and is extracted for 1 h at 40 °C with stirring. The insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production. In the soluble fraction, the pH is adjusted to 5.0, the temperature is lowered to 10 °C, and the precipitation process is carried out for 24 h. The resulting protein precipitate is separated from the liquid phase and washed twice with water pH 4 in a wet protein : water ratio of 1:2 and dried. The liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
[0022] Embodiment VI
[0023] In the embodiment, the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification. The raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method. The dehusked material is mechanically milled to a fraction with a size of 1 mm, suspended in extraction buffer pH 10 at a feedstock to buffer ratio of 1: 3 by weight and is extracted for 6 h at 50 °C with stirring. The insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production. In the soluble fraction, the pH is adjusted to 2,0, the temperature is lowered to 0 °C and the precipitation process is conducted for 1 h, the protein precipitate formed during the process is separated from the liquid phase and washed once with water pH 8 in a wet protein : water ratio of 1: 5, washed subsequently with a 90% ethanol solution pH 4 in a wet protein : water ratio of 1 : 1 and dried. The liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
[0024] Embodiment VII
[0025] In the embodiment, the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification. The raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method. The dehusked material is mechanically milled to a fraction with a size of 0, 1 mm, suspended in extraction buffer pH 9 at a feedstock to buffer ratio of 1:10 by weight and is extracted for 2 h at 40 °C with stirring. The insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production. In the soluble fraction, the pH is adjusted to 4.0, the temperature is lowered to 0 °C, and the precipitation process is carried out for 24 h. The resulting protein precipitate is separated from the liquid phase and washed with a 40% ethanol solution pH 4 in a wet protein : solution mass ratio 1:2 and dried. The liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
[0026] Embodiment VIII
[0027] In the embodiment, the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification. The raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method. The dehusked material is mechanically milled to a fraction with a size of 2 mm, suspended in extraction buffer pH 7 at a feedstock to buffer ratio of 1: 5 by weight and is extracted for 8 h at 20 °C with stirring. The insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production. In the soluble fraction, the pH is adjusted to 2.0, the temperature is lowered to 10 °C, and the precipitation process is carried out for 12 h. The protein precipitate obtained is separated from the liquid phase and washed three times with water pH 5 in a wet protein : water mass ratio of 1: 5, washed subsequently with a 90 % isopropanol solution pH 4 in a wet protein : water mass ratio of 1: 2 and dried. The liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
[0028] Embodiment IX
[0029] In the embodiment, the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification. The raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method. The dehusked material is mechanically milled to a fraction with a size of 0,1 mm, suspended in extraction buffer pH 10 at a feedstock to buffer ratio of 1: 15 by weight and is extracted for 1 h at 80 °C with stirring. The insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production. In the soluble fraction, the pH is adjusted to 6.0, the temperature is lowered to 0 °C, and the precipitation process is carried out for 1 h. The resulting protein precipitate is separated from the liquid phase and washed with a 60% isopropanol solution pH 8 in a wet protein : solution mass ratio 1:2 and dried. The liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
[0030] Embodiment X
[0031] In the embodiment, the method for processing legumes into protein having a nu tritional and feed value, bioethanol, biogas and fertiliser materials involves removing the husks, milling the dehusked seeds, extracting the protein into an aqueous solvent, precipitating the protein, washing the protein with an aqueous solution, washing the protein with a solvent solution, drying the protein, and subjecting the residue to ethanol fermentation and biogasification. The raw material in the form of pulses is subjected to a dehusking process, where the resulting husks are subjected to hydrolysis and biogasi fication using the CSTR method. The dehusked material is mechanically milled to a fraction with a size of 2 mm, suspended in extraction buffer pH 8 at a feedstock to buffer ratio of 1: 8 by weight and is extracted for 2 h at 30 °C with stirring. The insoluble fraction is separated from the soluble fraction and hydrolysed using enzymes into monosaccharides, followed by ethanol fermentation, where the resulting ethanol is distilled and the digestate is transferred as a substrate for biogas production. In the soluble fraction, the pH is adjusted to 3.0, the temperature is lowered to 20 °C, and the precipitation process is carried out for 8 h. The protein precipitate obtained is separated from the liquid phase and washed three times with water pH 5 in a wet protein : water mass ratio of 1: 3, washed subsequently with a 40% isopropanol solution pH 5 in a wet protein : water mass ratio of 1: 2 and dried. The liquid fraction is biogasified by CSTR, and the wet precipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.

Claims

Claims
[Claim 1] Method of processing raw plant material, especially legumes into protein having a nutritional and feed value, bioethanol, biogas and fertiliser materials, consisting in separating raw material in the form of husks, grinding the seed residues, extracting protein to a liquid phase, separating the solid phase and subjecting it to enzymatic hydrolysis, microbial fermentation and biogasification, characterised in that the raw plant material is dehusked, the resulting husks are subjected to hy drolysis and biogasification using the CSTR method, followed by the dehusked material being milled to a fraction with a size of 0.1 to 3 mm and extracted with stirring for 1 to 12 h at 20 to 80 °C, at pH 6 to 10 in excess extraction buffer at a range of 3 to 15 times the weight of the plant feedstock, wherein the insoluble solid fraction is separated and subjected to enzymatic hydrolysis with liquefying and saccharifying enzymes, followed by ethanol fermentation at a temperature of 25 to 40 °C for 24 to 72 h at pH 4 to 8, where the produced ethanol is distilled and the digestate is transferred as a substrate for biogas production, and the liquid fraction after the extraction process is subjected to a process of precipitation for 1 to 24 h at a temperature of 0 to 30 °C, pH 2 to 6 into a solid in the form of a protein precipitate, which is washed with an extraction buffer with a pH 4 to 8 in a solid fraction : buffer mass ratio of 1:2, after which the liquid residue is removed, which is subjected to a biogasification process using the CSTR method, and the wet pre cipitate produced after biogasification is used as a fertiliser or formed into dry pellets and used as an organic fertiliser.
[Claim 2] The method according to claim 1 characterised in that the solid form in the form of a protein sludge after the precipitation process is washed several times with an extraction buffer of pH 4 to 8 in a mass ratio of solid fraction: buffer 1: 2.
[Claim 3] The method according to claim 1 characterised in that the solid form in the form of a protein sludge after the precipitation process is washed with an aqueous solution with an organic solvent and dried to obtain the dry fraction.
[Claim 4] The method according to claim 3 characterised in that the solid form in the form of a protein sludge after the precipitation process is washed with an aqueous solution with ethanol at a concentration of 30 to 90%, pH 4 to 8, at a mass ratio of solid fraction: solution of 1: 2. [Claim 5] The method according to claim 1 characterised in that the solid form in the form of a protein sludge after the precipitation process is washed with an aqueous solution with 30 to 90% isopropanol at pH 4 to 8 in a mass ratio of solid fraction: solution of 1: 2.
PCT/PL2021/050022 2020-04-08 2021-04-07 A method for processing raw plant material, especially legumes into protein having a nutritional and feed value, bioethanol, biogas and fertiliser materials Ceased WO2021206573A1 (en)

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