WO2024112643A1 - Identification basée sur la fragmentomique d'états de modification du nombre de copies spécifiques à une tumeur dans une biopsie liquide - Google Patents

Identification basée sur la fragmentomique d'états de modification du nombre de copies spécifiques à une tumeur dans une biopsie liquide Download PDF

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WO2024112643A1
WO2024112643A1 PCT/US2023/080494 US2023080494W WO2024112643A1 WO 2024112643 A1 WO2024112643 A1 WO 2024112643A1 US 2023080494 W US2023080494 W US 2023080494W WO 2024112643 A1 WO2024112643 A1 WO 2024112643A1
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nucleic acid
loci
copy number
genomic loci
fragmentomic
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Alexander D. FINE
Brennan DECKER
Zheng KUANG
Chang Xu
Daokun SUN
Yanmei HUANG
Jason D. HUGHES
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Foundation Medicine Inc
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Foundation Medicine Inc
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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/10—Ploidy or copy number detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/20—Supervised data analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Definitions

  • the present disclosure relates generally to fragmentomics, and, more particularly, to methods and systems for identifying fragmentomics based tumor-specific copy number alteration states in liquid biopsy.
  • cfDNA Cell-free DNA in bodily fluids has precipitated the rapid development of cancer liquid biopsy.
  • plasma cfDNA may consist of a mixture of molecules originating from various bodily tissues, and the study and analysis of the fragmentation patterns of these cfDNA may generally be referred to as “fragmentomics.” Indeed, such fragmentation patterns of these cfDNA may be characterized by one or more fragmentomic metrics including, for example, fragment sizes, preferred ends, end motifs, single- stranded jagged ends, or nucleosomal footprints.
  • Genomic variant calling may generally include identifying single nucleotide polymorphisms (SNPs) and one or more base substitutions (e.g., subs), insertions or deletions (e.g., indels), copy number alterations (CNAs), and gene rearrangements, for example, by way of comprehensive genomic profiling (CGP) platform utilizing next generation sequencing (NGS) genomic data.
  • SNPs single nucleotide polymorphisms
  • CGP next generation sequencing
  • NGS next generation sequencing
  • one or more processors of a comprehensive genomic profiling (CGP) system may determine copy number alteration state for one or more genomic loci based on a liquid biopsy sample from a subject.
  • the one or more processors of the CGP system may receive sequence read data for a plurality of sequence reads derived from the liquid biopsy sample from the subject.
  • the one or more processors of the CGP system may then determine a normalized sequencing coverage depth for each of the one or more genomic loci based on the sequence read data.
  • the one or more processors of the CGP system may then determine a variant allele fraction for each of the one or more genomic loci based on the sequence read data.
  • the one or more processors of the CGP system may then determine one or more nucleic acid fragmentomic metrics for each of the one or more genomic loci based on the sequence read data. In certain embodiments, the one or more processors of the CGP system may then input the one or more nucleic acid fragmentomic metrics, the sequencing coverage depth, and the variant allele fraction determined for each of the one or more genomic loci into a copy number model trained to determine a copy number alteration state for each of the one or more genomic loci, and may then output the copy number alteration state determined for each of the one or more genomic loci.
  • the one or more nucleic acid fragmentomic metrics determined for each of the one or more genomic loci may be identified as being clinically -relevant based on an analysis of patient cohort data that includes clinical data, tissue sample sequencing data, liquid biopsy sample sequencing data, or any combination thereof, for patients that have been diagnosed with a disease.
  • a given nucleic acid fragmentomic metric of the one or more nucleic acid fragmentomic metrics may be identified as being clinically-relevant based on a comparison to a corresponding threshold that discriminates between clinically- relevant copy number alteration states and non-clinically-relevant copy number alteration states in the patient cohort data.
  • the disease may include a cancer.
  • the one or more genomic loci may include one or more single gene loci. In some embodiments, the one or more genomic loci comprise one or more genomic intervals. In some embodiments, the one or more genomic loci comprise one or more chromosomes, or portions thereof. In some embodiments, the normalized sequencing coverage depth comprises a log2 sequencing coverage depth.
  • the variant allele fraction determined for each of the one or more genomic loci may include a variant allele fraction determined for a plurality of germline single nucleotide polymorphisms (SNPs) located within the one or more genomic loci.
  • SNPs germline single nucleotide polymorphisms
  • the one or more nucleic acid fragmentomic metrics may include a median nucleic acid fragment length, a mean nucleic acid fragment length, a mode of nucleic acid fragment lengths, a weighted average of nucleic acid fragment lengths, a 25 th percentile of nucleic acid fragment lengths, a 75 th percentile of nucleic acid fragment lengths, a nucleic acid fragment start location, a nucleic acid fragment end location, a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a nucleic acid fragment end feature, or any combination thereof.
  • the one or more nucleic acid fragmentomic metrics may include a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • the one or more nucleic acid fragmentomic metrics comprise a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is determined based on an analysis of sequence read data for a control sample.
  • the control sample may include a process matched control.
  • the control sample may include a panel-of-normals control.
  • the one or more nucleic acid fragmentomic metrics may a nucleic acid fragment end metric and a nucleic acid fragment end-adjacent sequence metric, in which the nucleic acid fragment end metric may include a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at each end of the nucleic acid fragment.
  • the nucleic acid fragment end-adjacent sequence metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at points adjacent to each end of the nucleic acid fragment.
  • the copy number model may include a statistical model or other prediction-based model.
  • the copy number model may be trained on one or more training data sets including clinical data, normalized sequencing coverage depth data, variant allele fraction data, nucleic acid fragmentomic metric data, or any combination thereof, for a cohort of patients diagnosed with a disease.
  • the plurality of sequence reads may have been enriched for sequence reads derived from tumor cells by selecting sequence reads that are shorter than a second predetermined fragment length threshold.
  • the second predetermined fragment length threshold may be 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • the determination of copy number alteration state may include a qualitative determination of copy number gain, copy number loss, or copy number normal for the one or more genomic loci relative to reference genome.
  • the determination of copy number alteration state may include a quantitative determination of copy number for the one or more genomic loci.
  • the determination of copy number alteration state for each of the one or more genomic loci may be used to diagnose or confirm a diagnosis of disease in the subject.
  • the disease may include a cancer.
  • the one or more processors of the CGP system may select an anti-cancer therapy to administer to the subject based on the determination of copy number alteration state for each of the one or more genomic loci. In certain embodiments, the one or more processors of the CGP system may generate a prediction of a prognosis of the disease in the subject based on the determination of copy number alteration state for each of the one or more genomic loci. In certain embodiments, the one or more processors of the CGP system may determine an effective amount of the anti-cancer therapy to administer to the subject based on the determination of copy number alteration state for each of the one or more genomic loci.
  • the one or more processors of the CGP system may administer the anticancer therapy to the subject based on the determination of copy number alteration state for each of the one or more genomic loci.
  • the anti-cancer therapy may include chemotherapy, radiation therapy, immunotherapy, a targeted therapy, or surgery.
  • the one or more processors of the CGP system may further determine a variant allele frequency for each of a plurality of genomic loci based on the sequence read data. In certain embodiments, the one or more processors of the CGP system may further determine one or more nucleic acid fragmentomic metrics for each of the plurality of genomic loci based on the sequence read data. In certain embodiments, the one or more processors of the CGP system may further compare a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in the liquid biopsy sample to a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in a control sample.
  • the one or more processors of the CGP system may further determine a tumor fraction for the liquid biopsy sample based on the variant allele frequencies determined for the plurality of genomic loci and the comparison of the distributions of the one or more nucleic acid fragmentomic metrics. In certain embodiments, the one or more processors of the CGP system may further determine a germline allele frequency for each of a plurality of genomic loci based on the sequence read data, in which the germline allele frequency for each of the plurality of genomic loci comprises a determination of variant allele frequency for each of a plurality of single nucleotide polymorphisms (SNPs).
  • SNPs single nucleotide polymorphisms
  • the one or more nucleic acid fragmentomic metrics may include a median nucleic acid fragment length, a mean nucleic acid fragment length, a mode of nucleic acid fragment lengths, a weighted average of nucleic acid fragment lengths, a 25 th percentile of nucleic acid fragment lengths, a 75 th percentile of nucleic acid fragment lengths, a nucleic acid fragment start location, a nucleic acid fragment end location, a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a nucleic acid fragment end motif or feature, nucleic acid fragment end-adjacent sequence motifs of features, or any combination thereof.
  • the one or more nucleic acid fragmentomic metrics may include a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • the one or more nucleic acid fragmentomic metrics comprise a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, in which the first predetermined fragment length threshold is determined based on an analysis of sequence read data for a control sample.
  • the control sample comprises a panel-of-normals control.
  • the one or more nucleic acid fragmentomic metrics may include a nucleic acid fragment end metric and a nucleic acid fragment end-adjacent sequence metric, wherein the nucleic acid fragment end metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at each end of the nucleic acid fragment, and wherein the nucleic acid fragment end-adjacent sequence metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at points adjacent to each end of the nucleic acid fragment.
  • the comparison of the distributions of the one or more nucleic acid fragmentomic metrics for the liquid biopsy sample and the control sample may include determining a shift in a median nucleic acid fragment length, a shift in a mean nucleic acid fragment length, a shift in a mode of nucleic acid fragment lengths, a shift in a weighted average of nucleic acid fragment lengths, a shift in a 25 th percentile of nucleic acid fragment lengths, a shift in a 75 th percentile of nucleic acid fragment lengths, a shift in a nucleic acid fragment start location, a shift in a nucleic acid fragment end location, a shift in a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a shift in a nucleic acid fragment end metric, or any combination thereof.
  • the determination of tumor fraction for the liquid biopsy sample is used to diagnose or confirm a diagnosis of disease in the subject.
  • FIG. 1 illustrates an illustrative example for identifying fragmentomics based tumorspecific copy number alteration states in a liquid biopsy sample, according to some embodiments.
  • FIG. 2 illustrates an example CGP system that may be utilized for determining copy number alteration states for one or more genomic loci based on a liquid biopsy sample, according to some embodiments.
  • FIG. 3 illustrates a flow diagram of a method for determining copy number alteration states for one or more genomic loci based on a liquid biopsy sample, according to some embodiments.
  • FIG. 4 illustrates a flow diagram of a method for determining a tumor fraction for a liquid biopsy sample, according to some embodiments.
  • FIG. 5A illustrates a flow diagram of a method for monitoring cancer progression or recurrence in a subject, according to some embodiments.
  • FIG. 5B illustrates a flow diagram of a method for monitoring cancer progression or recurrence in a subject, according to some embodiments.
  • FIG. 6 illustrates an example computing system, according to some embodiments.
  • one or more processors of a comprehensive genomic profiling (CGP) system may determine copy number alteration state for one or more genomic loci based on a liquid biopsy sample from a subject.
  • the one or more processors of the CGP system may receive sequence read data for a plurality of sequence reads derived from the liquid biopsy sample from the subject.
  • the one or more processors of the CGP system may then determine a normalized sequencing coverage depth for each of the one or more genomic loci based on the sequence read data.
  • the one or more processors of the CGP system may then determine a variant allele fraction for each of the one or more genomic loci based on the sequence read data.
  • the one or more processors of the CGP system may then determine one or more nucleic acid fragmentomic metrics for each of the one or more genomic loci based on the sequence read data. In certain embodiments, the one or more processors of the CGP system may then input the one or more nucleic acid fragmentomic metrics, the sequencing coverage depth, and the variant allele fraction determined for each of the one or more genomic loci into a copy number model trained to determine a copy number alteration state for each of the one or more genomic loci, and may then output the copy number alteration state determined for each of the one or more genomic loci.
  • the one or more nucleic acid fragmentomic metrics determined for each of the one or more genomic loci may be identified as being clinically-relevant based on an analysis of patient cohort data that includes clinical data, tissue sample sequencing data, liquid biopsy sample sequencing data, or any combination thereof, for patients that have been diagnosed with a disease.
  • the one or more gene loci comprises between 10 and 20 loci, between 10 and 40 loci, between 10 and 60 loci, between 10 and 80 loci, between 10 and 100 loci, between 10 and 150 loci, between 10 and 200 loci, between 10 and 250 loci, between 10 and 300 loci, between 10 and 350 loci, between 10 and 400 loci, between 10 and 450 loci, between 10 and 500 loci, between 20 and 40 loci, between 20 and 60 loci, between 20 and 80 loci, between 20 and 100 loci, between 20 and 150 loci, between 20 and 200 loci, between 20 and 250 loci, between 20 and 300 loci, between 20 and 350 loci, between 20 and 400 loci, between 20 and 500 loci, between 40 and 60 loci, between 40 and 80 loci, between 40 and 100 loci, between 40 and 150 loci, between 40 and 200 loci, between 40 and 250 loci, between 40 and 300 loci, between 40 and 350 loci, between 40 and 400 loci, between 40 and 500 loci, between 40 and 60 loci,
  • the one or more gene loci may include ABL1, ACVR1B, AKT1, AKT2, AKT3, ALK, ALOX12B, AMER1, APC, AR, ARAF, ARFRP1, ARID1A, ASXL1, ATM, ATR, ATRX, AURKA, AURKB, AXIN1, AXL, BAP1, BARD1, BCL2, BCL2L1, BCL2L2, BCL6, BCOR, BCORL1, BCR, BRAF, BRCA1, BRCA2, BRD4, BRIP1, BTG1, BTG2, BTK, CALR, CARD11, CASP8, CBFB, CBL, CCND1, CCND2, CCND3, CCNE1, CD22, CD274, CD70, CD74, CD79A, CD79B, CDC73, CDH1, CDK12, CDK4, CDK6, CDK8, CDKN1A, CDKN1B, CDKN2A, CDKN2B, CDKN2C, CEBPA
  • the one or more gene loci may include ABL, ALK, ALL, B4GALNT1, BAFF, BCL2, BRAF, BRCA, BTK, CD19, CD20, CD3, CD30, CD319, CD38, CD52, CDK4, CDK6, CML, CRACC, CS1, CTLA-4, dMMR, EGFR, ERBB1, ERBB2, FGFR1- 3, FLT3, GD2, HDAC, HER1, HER2, HR, IDH2, IL-ip, IL-6, IL-6R, JAK1, JAK2, JAK3, KIT, KRAS, MEK, MET, MSLH, mTOR, PARP, PD-1, PDGFR, PDGFRa, PDGFRp, PD-L1, PI3K5, PIGF, PTCH, RAF, RANKL, RET, ROS1, SLAMF7, VEGF, VEGFA, VEGFB, or any combination thereof.
  • a given nucleic acid fragmentomic metric of the one or more nucleic acid fragmentomic metrics may be identified as being clinically-relevant based on a comparison to a corresponding threshold that discriminates between clinically- relevant copy number alteration states and non-clinically-relevant copy number alteration states in the patient cohort data.
  • the disease may include a cancer.
  • the cancer may include acute lymphoblastic leukemia (Philadelphia chromosome positive), acute lymphoblastic leukemia (precursor B-cell), acute myeloid leukemia (FLT3+), acute myeloid leukemia (with an IDH2 mutation), anaplastic large cell lymphoma, basal cell carcinoma, B-cell chronic lymphocytic leukemia, bladder cancer, breast cancer (HER2 overexpressed/amplified), breast cancer (HER2+), breast cancer (HR+, HER2-), cervical cancer, cholangiocarcinoma, chronic lymphocytic leukemia, chronic lymphocytic leukemia (with 17p deletion), chronic myelogenous leukemia, chronic myelogenous leukemia (Philadelphia chromosome positive), classical Hodgkin lymphoma, colorectal cancer, colorectal cancer (dMMR/MSI-H), colorectal cancer (KRA), acute lymphoblastic leukemia (
  • the one or more genomic loci may include one or more single gene loci. In some embodiments, the one or more genomic loci comprise one or more genomic intervals. In some embodiments, the one or more genomic loci comprise one or more chromosomes, or portions thereof. In some embodiments, the normalized sequencing coverage depth comprises a log2 sequencing coverage depth.
  • the variant allele fraction determined for each of the one or more genomic loci may include a variant allele fraction determined for a plurality of germline single nucleotide polymorphisms (SNPs) located within the one or more genomic loci.
  • SNPs germline single nucleotide polymorphisms
  • the one or more nucleic acid fragmentomic metrics may include a median nucleic acid fragment length, a mean nucleic acid fragment length, a mode of nucleic acid fragment lengths, a weighted average of nucleic acid fragment lengths, a 25 th percentile of nucleic acid fragment lengths, a 75 th percentile of nucleic acid fragment lengths, a nucleic acid fragment start location, a nucleic acid fragment end location, a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a nucleic acid fragment end feature, or any combination thereof.
  • the one or more nucleic acid fragmentomic metrics may include a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • the one or more nucleic acid fragmentomic metrics comprise a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is determined based on an analysis of sequence read data for a control sample.
  • the control sample may include a process matched control.
  • the control sample may include a panel-of-normals control.
  • the one or more nucleic acid fragmentomic metrics may a nucleic acid fragment end metric and a nucleic acid fragment end-adjacent sequence metric, in which the nucleic acid fragment end metric may include a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at each end of the nucleic acid fragment.
  • the nucleic acid fragment end-adjacent sequence metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at points adjacent to each end of the nucleic acid fragment.
  • the copy number model may be trained on one or more training data sets including clinical data, normalized sequencing coverage depth data, variant allele fraction data, nucleic acid fragmentomic metric data, or any combination thereof, for a cohort of patients diagnosed with a disease.
  • the plurality of sequence reads may have been enriched for sequence reads derived from tumor cells by selecting sequence reads that are shorter than a second predetermined fragment length threshold.
  • the second predetermined fragment length threshold may be 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • the determination of copy number alteration state may include a qualitative determination of copy number gain, copy number loss, or copy number normal for the one or more genomic loci relative to reference genome.
  • the determination of copy number alteration state may include a quantitative determination of copy number for the one or more genomic loci.
  • the determination of copy number alteration state for each of the one or more genomic loci may be used to diagnose or confirm a diagnosis of disease in the subject.
  • the disease may include a cancer.
  • the one or more processors of the CGP system may select an anti-cancer therapy to administer to the subject based on the determination of copy number alteration state for each of the one or more genomic loci. In certain embodiments, the one or more processors of the CGP system may generate a prediction of a prognosis of the disease in the subject based on the determination of copy number alteration state for each of the one or more genomic loci. In certain embodiments, the one or more processors of the CGP system may determine an effective amount of the anti-cancer therapy to administer to the subject based on the determination of copy number alteration state for each of the one or more genomic loci.
  • the one or more processors of the CGP system may administer the anticancer therapy to the subject based on the determination of copy number alteration state for each of the one or more genomic loci.
  • the anti-cancer therapy may include chemotherapy, radiation therapy, immunotherapy, a targeted therapy, or surgery.
  • the targeted anti-cancer therapy may include abemaciclib (Verzenio), abiraterone acetate (Zytiga), acalabrutinib (Calquence), ado-trastuzumab emtansine (Kadcyla), afatinib dimaleate (Gilotrif), aldesleukin (Proleukin), alectinib (Alecensa), alemtuzumab (Campath), alitretinoin (Panretin), alpelisib (Piqray), amivantamab-vmjw (Rybrevant), anastrozole (Arimidex), apalutamide (Erleada), asciminib hydrochloride (Scemblix), atezolizumab (Tecentriq), avapritinib (Ayvakit), avelumab (Bavencio), axicabtagen
  • the one or more processors of the CGP system may generate a report indicating the copy number alteration state determined for each of the one or more genomic loci. In some embodiments, the one or more processors of the CGP system may transmit the report to a healthcare provider. In some embodiments, the one or more processors of the CGP system may transmit the report via a computer network or a peer-to-peer connection.
  • the one or more processors of the CGP system may further determine a variant allele frequency for each of a plurality of genomic loci based on the sequence read data. In certain embodiments, the one or more processors of the CGP system may further determine one or more nucleic acid fragmentomic metrics for each of the plurality of genomic loci based on the sequence read data. In certain embodiments, the one or more processors of the CGP system may further compare a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in the liquid biopsy sample to a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in a control sample.
  • the one or more processors of the CGP system may further determine a tumor fraction for the liquid biopsy sample based on the variant allele frequencies determined for the plurality of genomic loci and the comparison of the distributions of the one or more nucleic acid fragmentomic metrics. In certain embodiments, the one or more processors of the CGP system may further determine a germline allele frequency for each of a plurality of genomic loci based on the sequence read data, in which the germline allele frequency for each of the plurality of genomic loci comprises a determination of variant allele frequency for each of a plurality of single nucleotide polymorphisms (SNPs).
  • SNPs single nucleotide polymorphisms
  • the one or more nucleic acid fragmentomic metrics may include a median nucleic acid fragment length, a mean nucleic acid fragment length, a mode of nucleic acid fragment lengths, a weighted average of nucleic acid fragment lengths, a 25 th percentile of nucleic acid fragment lengths, a 75 th percentile of nucleic acid fragment lengths, a nucleic acid fragment start location, a nucleic acid fragment end location, a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a nucleic acid fragment end motif or feature, nucleic acid fragment end-adjacent sequence motifs of features, or any combination thereof.
  • the one or more nucleic acid fragmentomic metrics may include a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • the one or more nucleic acid fragmentomic metrics comprise a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, in which the first predetermined fragment length threshold is determined based on an analysis of sequence read data for a control sample.
  • the control sample comprises a panel-of-normals control.
  • the one or more nucleic acid fragmentomic metrics may include a nucleic acid fragment end metric and a nucleic acid fragment end-adjacent sequence metric, wherein the nucleic acid fragment end metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at each end of the nucleic acid fragment, and wherein the nucleic acid fragment end-adjacent sequence metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at points adjacent to each end of the nucleic acid fragment.
  • the comparison of the distributions of the one or more nucleic acid fragmentomic metrics for the liquid biopsy sample and the control sample may include determining a shift in a median nucleic acid fragment length, a shift in a mean nucleic acid fragment length, a shift in a mode of nucleic acid fragment lengths, a shift in a weighted average of nucleic acid fragment lengths, a shift in a 25 th percentile of nucleic acid fragment lengths, a shift in a 75 th percentile of nucleic acid fragment lengths, a shift in a nucleic acid fragment start location, a shift in a nucleic acid fragment end location, a shift in a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a shift in a nucleic acid fragment end metric, or any combination thereof.
  • the determination of tumor fraction for the liquid biopsy sample is used to diagnose or confirm a diagnosis of disease in the subject.
  • the terms “comprising” (and any form or variant of comprising, such as “comprise” and “comprises”), “having” (and any form or variant of having, such as “have” and “has”), “including” (and any form or variant of including, such as “includes” and “include”), or “containing” (and any form or variant of containing, such as “contains” and “contain”), are inclusive or open-ended and do not exclude additional, un-recited additives, components, integers, elements, or method steps.
  • subgenomic interval refers to a portion of a genomic sequence.
  • subject interval refers to a subgenomic interval or an expressed subgenomic interval (e.g., the transcribed sequence of a subgenomic interval).
  • variant sequence As used herein, the terms “variant sequence” or “variant” are used interchangeably and refer to a modified nucleic acid sequence relative to a corresponding “normal” or “wild-type” sequence. In some instances, a variant sequence may be a “short variant sequence” (or “short variant”), i.e., a variant sequence of less than about 50 base pairs in length.
  • allele frequency and “allele fraction” are used interchangeably herein and refer to the fraction of sequence reads corresponding to a particular allele relative to the total number of sequence reads for a genomic locus.
  • variant allele frequency and “variant allele fraction” are used interchangeably herein and refer to the fraction of sequence reads corresponding to a particular variant allele relative to the total number of sequence reads for a genomic locus.
  • clinical relevant and “clinically significant” are used interchangeably and refer to any information relevant to diagnosis and/or treatment of a disease (e.g., cancer) of a patient, including but not limited to information that may be of a highest priority or relevance to the diagnosis and/or treatment of the disease based on a disease state of the patient.
  • a disease e.g., cancer
  • medical information refers to one or more therapeutic, diagnostic, prognostic, potential germline, potential clonal hematopoiesis, or other related information based, at least in part, on a patient’s medical information.
  • one or more processors of a comprehensive genomic profiling (CGP) system may determine copy number alteration state for one or more genomic loci based on a liquid biopsy sample from a subject.
  • the one or more processors of the CGP system may receive sequence read data for a plurality of sequence reads derived from the liquid biopsy sample from the subject.
  • the one or more processors of the CGP system may then determine a normalized sequencing coverage depth for each of the one or more genomic loci based on the sequence read data.
  • the one or more processors of the CGP system may then determine a variant allele fraction for each of the one or more genomic loci based on the sequence read data.
  • the one or more processors of the CGP system may then determine one or more nucleic acid fragmentomic metrics for each of the one or more genomic loci based on the sequence read data. In certain embodiments, the one or more processors of the CGP system may then input the one or more nucleic acid fragmentomic metrics, the sequencing coverage depth, and the variant allele fraction determined for each of the one or more genomic loci into a copy number model trained to determine a copy number alteration state for each of the one or more genomic loci, and may then output the copy number alteration state determined for each of the one or more genomic loci.
  • the one or more nucleic acid fragmentomic metrics determined for each of the one or more genomic loci may be identified as being clinically -relevant based on an analysis of patient cohort data that includes clinical data, tissue sample sequencing data, liquid biopsy sample sequencing data, or any combination thereof, for patients that have been diagnosed with a disease.
  • a given nucleic acid fragmentomic metric of the one or more nucleic acid fragmentomic metrics may be identified as being clinically-relevant based on a comparison to a corresponding threshold that discriminates between clinically- relevant copy number alteration states and non-clinically-relevant copy number alteration states in the patient cohort data.
  • the disease may include a cancer.
  • the one or more genomic loci may include one or more single gene loci. In some embodiments, the one or more genomic loci comprise one or more genomic intervals. In some embodiments, the one or more genomic loci comprise one or more chromosomes, or portions thereof. In some embodiments, the normalized sequencing coverage depth comprises a log2 sequencing coverage depth.
  • the variant allele fraction determined for each of the one or more genomic loci may include a variant allele fraction determined for a plurality of germline single nucleotide polymorphisms (SNPs) located within the one or more genomic loci.
  • SNPs germline single nucleotide polymorphisms
  • the one or more nucleic acid fragmentomic metrics may include a median nucleic acid fragment length, a mean nucleic acid fragment length, a mode of nucleic acid fragment lengths, a weighted average of nucleic acid fragment lengths, a 25 th percentile of nucleic acid fragment lengths, a 75 th percentile of nucleic acid fragment lengths, a nucleic acid fragment start location, a nucleic acid fragment end location, a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a nucleic acid fragment end feature, or any combination thereof.
  • the one or more nucleic acid fragmentomic metrics may include a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • the one or more nucleic acid fragmentomic metrics comprise a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is determined based on an analysis of sequence read data for a control sample.
  • the control sample may include a process matched control.
  • the control sample may include a panel-of-normals control.
  • the one or more nucleic acid fragmentomic metrics may a nucleic acid fragment end metric and a nucleic acid fragment end-adjacent sequence metric, in which the nucleic acid fragment end metric may include a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at each end of the nucleic acid fragment.
  • the nucleic acid fragment end-adjacent sequence metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at points adjacent to each end of the nucleic acid fragment.
  • the copy number model may be trained on one or more training data sets including clinical data, normalized sequencing coverage depth data, variant allele fraction data, nucleic acid fragmentomic metric data, or any combination thereof, for a cohort of patients diagnosed with a disease.
  • the disclosed methods may be used to identify variants in the ABL1, ACVR1B, AKT1, AKT2, AKT3, ALK, ALOX12B, AMER1, APC, AR, ARAF, ARFRP1, ARID1A, ASXL1, ATM, ATR, ATRX, AURKA, AURKB, AXIN1, AXL, BAP1, BARD1, BCL2, BCL2L1, BCL2L2, BCL6, BCOR, BCORL1, BCR, BRAF, BRCA1, BRCA2, BRD4, BRIP1, BTG1, BTG2, BTK, CALR, CARD11, CASP8, CBFB, CBL, CCND1, CCND2, CCND3, CCNE1, CD22, CD274, CD70, CD74, CD79A, CD79B, CDC73, CDH1, CDK12, CDK4, CDK6, CDK8, CDKN1A, CDKN1B, CDKN2A, CDKN2B, CDKN2C,
  • the disclosed methods may be used to identify variants in the ABL, ALK, ALL, B4GALNT1, BAFF, BCL2, BRAF, BRCA, BTK, CD19, CD20, CD3, CD30, CD319, CD38, CD52, CDK4, CDK6, CML, CRACC, CS1, CTLA-4, dMMR, EGFR, ERBB1, ERBB2, FGFR1-3, FLT3, GD2, HDAC, HER1, HER2, HR, IDH2, IL-ip, IL-6, IL-6R, JAK1, JAK2, JAK3, KIT, KRAS, MEK, MET, MSLH, mTOR, PARP, PD-1, PDGFR, PDGFRa, PDGFRP, PD-L1, PI3K5, PIGF, PTCH, RAF, RANKL, RET, ROS1, SLAMF7, VEGF, VEGFA, or VEGFB gene locus, or any combination
  • the disclosed methods may be used to identify variants other than ABL, ALK, ALL, B4GALNT1, BAFF, BCL2, BRAF, BRCA, BTK, CD19, CD20, CD3, CD30, CD319, CD38, CD52, CDK4, CDK6, CML, CRACC, CS1, CTLA-4, dMMR, EGFR, ERBB1, ERBB2, FGFR1-3, FLT3, GD2, HDAC, HER1, HER2, HR, IDH2, IL-ip, IL-6, IL-6R, JAK1, JAK2, JAK3, KIT, KRAS, MEK, MET, MSLH, mTOR, PARP, PD-1, PDGFR, PDGFRa, PDGFRp, PD-L1, PI3K5, PIGF, PTCH, RAF, RANKL, RET, ROS1, SLAMF7, VEGF, VEGFA, or VEGFB gene locus, or any combination thereof.
  • mTOR PAR
  • the plurality of sequence reads may have been enriched for sequence reads derived from tumor cells by selecting sequence reads that are shorter than a second predetermined fragment length threshold.
  • the second predetermined fragment length threshold may be 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • the determination of copy number alteration state may include a qualitative determination of copy number gain, copy number loss, or copy number normal for the one or more genomic loci relative to reference genome.
  • the determination of copy number alteration state may include a quantitative determination of copy number for the one or more genomic loci.
  • the determination of copy number alteration state for each of the one or more genomic loci may be used to diagnose or confirm a diagnosis of disease in the subject.
  • the disease may include a cancer.
  • the one or more processors of the CGP system may select an anti-cancer therapy to administer to the subject based on the determination of copy number alteration state for each of the one or more genomic loci. In certain embodiments, the one or more processors of the CGP system may generate a prediction of a prognosis of the disease in the subject based on the determination of copy number alteration state for each of the one or more genomic loci. In certain embodiments, the one or more processors of the CGP system may determine an effective amount of the anti-cancer therapy to administer to the subject based on the determination of copy number alteration state for each of the one or more genomic loci.
  • the one or more processors of the CGP system may administer the anticancer therapy to the subject based on the determination of copy number alteration state for each of the one or more genomic loci.
  • the anti-cancer therapy may include chemotherapy, radiation therapy, immunotherapy, a targeted therapy, or surgery.
  • the one or more processors of the CGP system may further determine a variant allele frequency for each of a plurality of genomic loci based on the sequence read data. In certain embodiments, the one or more processors of the CGP system may further determine one or more nucleic acid fragmentomic metrics for each of the plurality of genomic loci based on the sequence read data. In certain embodiments, the one or more processors of the CGP system may further compare a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in the liquid biopsy sample to a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in a control sample.
  • the one or more processors of the CGP system may further determine a tumor fraction for the liquid biopsy sample based on the variant allele frequencies determined for the plurality of genomic loci and the comparison of the distributions of the one or more nucleic acid fragmentomic metrics. In certain embodiments, the one or more processors of the CGP system may further determine a germline allele frequency for each of a plurality of genomic loci based on the sequence read data, in which the germline allele frequency for each of the plurality of genomic loci comprises a determination of variant allele frequency for each of a plurality of single nucleotide polymorphisms (SNPs).
  • SNPs single nucleotide polymorphisms
  • the one or more nucleic acid fragmentomic metrics may include a median nucleic acid fragment length, a mean nucleic acid fragment length, a mode of nucleic acid fragment lengths, a weighted average of nucleic acid fragment lengths, a 25 th percentile of nucleic acid fragment lengths, a 75 th percentile of nucleic acid fragment lengths, a nucleic acid fragment start location, a nucleic acid fragment end location, a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a nucleic acid fragment end motif or feature, nucleic acid fragment end-adjacent sequence motifs of features, or any combination thereof.
  • the one or more nucleic acid fragmentomic metrics may include a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • the one or more nucleic acid fragmentomic metrics comprise a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, in which the first predetermined fragment length threshold is determined based on an analysis of sequence read data for a control sample.
  • the control sample comprises a panel-of-normals control.
  • the one or more nucleic acid fragmentomic metrics may include a nucleic acid fragment end metric and a nucleic acid fragment end-adjacent sequence metric, wherein the nucleic acid fragment end metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at each end of the nucleic acid fragment, and wherein the nucleic acid fragment end-adjacent sequence metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at points adjacent to each end of the nucleic acid fragment.
  • the comparison of the distributions of the one or more nucleic acid fragmentomic metrics for the liquid biopsy sample and the control sample may include determining a shift in a median nucleic acid fragment length, a shift in a mean nucleic acid fragment length, a shift in a mode of nucleic acid fragment lengths, a shift in a weighted average of nucleic acid fragment lengths, a shift in a 25 th percentile of nucleic acid fragment lengths, a shift in a 75 th percentile of nucleic acid fragment lengths, a shift in a nucleic acid fragment start location, a shift in a nucleic acid fragment end location, a shift in a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a shift in a nucleic acid fragment end metric, or any combination thereof.
  • the determination of tumor fraction for the liquid biopsy sample is used to diagnose or confirm a diagnosis of disease in the subject.
  • FIG. 1 illustrates an illustrative example 100 for identifying fragmentomics based tumorspecific copy number alteration states in liquid biopsy, in accordance with the disclosed embodiments.
  • the illustrative example 100 may generally depict a single-locus copy number calling embodiment or genome- wide copy number calling embodiment.
  • fragmentomic metrics from a genomic region e.g., PTEN locus
  • flanking regions with no copy number changes is compared to flanking regions with no copy number changes.
  • the fragmentomic metrics may include, for example, median fragment length, mean fragment length, mode of the fragment lengths, 25 th percentile of fragment lengths, 75 th percentile of fragment lengths, fragment start and end genomic location, or percentage of fragments shorter than a given length threshold.
  • genomic regions that are deleted in the tumor may not be present, and thus may not shed short fragments from lost genomic regions. These regions may be expected to exhibit longer fragment length metrics than regions without copy number losses.
  • genomic regions with copy gains may be expected to shed an excess of short fragments and these genomic regions may be expected to have shorter fragment length metrics than regions without copy number gains.
  • FIG. 2 illustrates an example CGP system 200 that may be utilized for determining copy number alteration states for one or more genomic loci based on a liquid biopsy sample, in accordance with the disclosed embodiments.
  • single-locus copy number calling embodiments or genome- wide copy number calling embodiments for including fragmentomics metrics in single-locus copy number calls or genomewide copy number calls may include a process of, for example, collecting normalized depth of coverage and variant allele fraction metrics, and extracting sample-level fragmentomic metrics from a binary alignment map (BAM) file for intra-sample and/or cross-sample comparisons.
  • BAM binary alignment map
  • the process may further include, for example, generating a sample-level genomic map of fragment length metrics, selecting sequence reads from the BAM file which overlap with a gene or genomic interval of interest, and comparing fragmentomic metrics for the region of interest with intra-sample and cross-sample points of reference.
  • the process may further include quantifying key features for calling clinically-significant copy gains and losses, and inputting the collected normalized depth of coverage and variant allele fraction metrics and sample-level fragmentomic metrics into a copy number model trained to generate one or more predictions of copy number states.
  • the CGP system 200 may include, for example, one or more laboratory processes 202 and a computing process 204, respectively.
  • the one or more laboratory processes 202 may include a process, in which a cfDNA sequence may be prepared to be sequenced.
  • a liquid biopsy sample from a subject may be obtained, and cfDNA may be isolated or extracted from the liquid biopsy sample.
  • the one or more laboratory processes 202 may further include a process, in which, for example, one or more sequence reads 206 may be extracted from the cfDNA sequence.
  • the one or more sequence reads 206 may be included in a BAM file or sorted BAM file, in which the BAM file or sorted BAM file may include, for example, any targeted or baited sequencing reads extracted from the liquid biopsy sample.
  • the computing process 204 may begin by accessing the one or more sequence reads 206 included in the BAM file or sorted BAM file, and utilizing the one or more sequence reads 206 to determine, for example, a normalized sequencing coverage depth 208 for each of one or more genomic loci, a variant allele fraction 210 for each of the one or more genomic loci, and one or more nucleic acid fragmentomic metrics 212 for each of the one or more genomic loci.
  • the normalized sequencing coverage depth 208, the variant allele fraction 210, and the one or more nucleic acid fragmentomic metrics 212 may be then inputted to a copy number model 214, which may be trained to generate one or more predictions of copy number alteration states 216 in accordance with the presently disclosed embodiments.
  • the copy number model 214 may include, for example, any statistical model or other similar prediction-based model that may be suitable for generating predictions of any number of copy number states in accordance with the presently disclosed embodiments.
  • the copy number model 214 may be trained to generate the one or more predictions of copy number alteration states 216 for each of the one or more genomic loci. In this way, the present embodiments may predict copy number alteration states for one or more genomic loci based on a liquid biopsy sample for both singlelocus copy number calling and genome- wide copy number calling.
  • FIG. 3 illustrates a flow diagram of a method 300 for determining copy number alteration states for one or more genomic loci based on a liquid biopsy sample, in accordance with the disclosed embodiments.
  • the method 300 may, at least in part, be performed utilizing one or more processing devices (e.g., computing device(s) to be discussed below with respect to FIG.
  • 6) that may include hardware (e.g., a general purpose processor, a graphic processing unit (GPU), an application-specific integrated circuit (ASIC), a system-on- chip (SoC), a microcontroller, a field-programmable gate array (FPGA), a central processing unit (CPU), an application processor (AP), a visual processing unit (VPU), a neural processing unit (NPU), a neural decision processor (NDP), a deep learning processor (DLP), a tensor processing unit (TPU), a neuromorphic processing unit (NPU), or any other artificial-intelligence (Al) accelerator processing device(s) that may be suitable for instructions running/executing on one or more processors), firmware (e.g., microcode), or some combination thereof.
  • hardware e.g., a general purpose processor, a graphic processing unit (GPU), an application- specific integrated circuit (ASIC), a system-on- chip (SoC), a microcontroller, a field-programmable gate array (FPGA), a central processing
  • the method 300 may begin at block 302 with one or more processing devices receiving sequence read data for the plurality of sequence reads.
  • the method 300 may continue at block 304 with the one or more processing devices determining a normalized sequencing coverage depth for each of the one or more genomic loci based on the sequence read data.
  • the method 300 may continue at block 306 with the one or more processing devices determining a variant allele fraction for each of the one or more genomic loci based on the sequence read data.
  • the method 300 may continue at block 308 with the one or more processing devices determining one or more nucleic acid fragmentomic metrics for each of the one or more genomic loci based on the sequence read data.
  • the method 300 may continue at block 310 with the one or more processing devices inputting the one or more nucleic acid fragmentomic metrics, the sequencing coverage depth, and the variant allele fraction determined for each of the one or more genomic loci into a copy number model trained to determine a copy number alteration state for each of the one or more genomic loci.
  • the method 300 may conclude at block 312 with the one or more processing devices outputting the copy number alteration state determined for each of the one or more genomic loci.
  • FIG. 4 illustrates a flow diagram of a method 400 for determining a tumor fraction for a liquid biopsy sample, in accordance with the disclosed embodiments.
  • the method 400 may, at least in part, be performed utilizing one or more processing devices (e.g., computing device(s) to be discussed below with respect to FIG.
  • 6) that may include hardware (e.g., a general purpose processor, a graphic processing unit (GPU), an application-specific integrated circuit (ASIC), a system-on-chip (SoC), a microcontroller, a field-programmable gate array (FPGA), a central processing unit (CPU), an application processor (AP), a visual processing unit (VPU), a neural processing unit (NPU), a neural decision processor (NDP), a deep learning processor (DLP), a tensor processing unit (TPU), a neuromorphic processing unit (NPU), or any other artificial-intelligence (Al) accelerator processing device(s) that may be suitable for instructions running/executing on one or more processors), firmware (e.g., microcode), or some combination thereof.
  • hardware e.g., a general purpose processor, a graphic processing unit (GPU), an application- specific integrated circuit (ASIC), a system-on-chip (SoC), a microcontroller, a field-programmable gate array (FPGA), a central processing
  • the method 400 may begin at block 402 with one or more processing devices receiving sequence read data for a plurality of sequence reads derived from the liquid biopsy sample.
  • the method 400 may continue at block 404 with the one or more processing devices determining a variant allele frequency for each of a plurality of genomic loci based on the sequence read data.
  • the method 400 may continue at block 406 with the one or more processing devices determining one or more nucleic acid fragmentomic metrics for each of the plurality of genomic loci based on the sequence read data.
  • the method 400 may continue at block 408 with the one or more processing devices comparing distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in the liquid biopsy sample to a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in a control sample.
  • the method 400 may continue at block 410 with the one or more processing devices determining a tumor fraction for the liquid biopsy sample based on the variant allele frequencies determined for the plurality of genomic loci and the comparison of the distributions of the one or more nucleic acid fragmentomic metrics.
  • FIG. 5A illustrates a flow diagram of a method 500A for monitoring cancer progression or recurrence in a subject, in accordance with the disclosed embodiments.
  • the method 500A may, at least in part, be performed utilizing one or more processing devices (e.g., computing device(s) to be discussed below with respect to FIG.
  • 6) that may include hardware (e.g., a general purpose processor, a graphic processing unit (GPU), an application-specific integrated circuit (ASIC), a system-on-chip (SoC), a microcontroller, a field- programmable gate array (FPGA), a central processing unit (CPU), an application processor (AP), a visual processing unit (VPU), a neural processing unit (NPU), a neural decision processor (NDP), a deep learning processor (DLP), a tensor processing unit (TPU), a neuromorphic processing unit (NPU), or any other artificial-intelligence (Al) accelerator processing device(s) that may be suitable for instructions running/executing on one or more processors), firmware (e.g., microcode), or some combination thereof.
  • hardware e.g., a general purpose processor, a graphic processing unit (GPU), an application-specific integrated circuit (ASIC), a system-on-chip (SoC), a microcontroller, a field- programmable gate array (FPGA), a
  • the method 500A may begin at block 502 with one or more processing devices determining a first copy number alteration state for each of one or more genomic loci in a first liquid biopsy sample obtained from the subject at a first time point according to the method of any one of claims 31 to 57 below.
  • the method 500A may continue at block 504 with the one or more processing devices determining a second copy number alteration state for each of the one or more genomic loci in a second liquid biopsy sample obtained from the subject at a second time point.
  • the method 500A may conclude at block 506 with the one or more processing devices comparing the first determination of copy number alteration state to the second determination of copy number alteration state for each of the one or more genomic loci, thereby monitoring the cancer progression or recurrence.
  • FIG. 5B illustrates a flow diagram of a method 500B for monitoring cancer progression or recurrence in a subject, in accordance with the disclosed embodiments.
  • the method 500B may, at least in part, be performed utilizing one or more processing devices (e.g., computing device(s) to be discussed below with respect to FIG.
  • 6) that may include hardware (e.g., a general purpose processor, a graphic processing unit (GPU), an application-specific integrated circuit (ASIC), a system-on-chip (SoC), a microcontroller, a field- programmable gate array (FPGA), a central processing unit (CPU), an application processor (AP), a visual processing unit (VPU), a neural processing unit (NPU), a neural decision processor (NDP), a deep learning processor (DLP), a tensor processing unit (TPU), a neuromorphic processing unit (NPU), or any other artificial-intelligence (Al) accelerator processing device(s) that may be suitable for instructions running/executing on one or more processors), firmware (e.g., microcode), or some combination thereof.
  • hardware e.g., a general purpose processor, a graphic processing unit (GPU), an application-specific integrated circuit (ASIC), a system-on-chip (SoC), a microcontroller, a field- programmable gate array (FPGA), a
  • the method 500B may begin at block 508 with one or more processing devices determining a first tumor fraction in a first liquid biopsy sample obtained from the subject at a first time point according to the method of any one of claims 58 to 72 below.
  • the method 500B may continue at block 510 with the one or more processing devices determining a second tumor fraction in a second liquid biopsy sample obtained from the subject at a second time point.
  • the method 500B may conclude at block 512 with the one or more processing devices comparing the first determination of tumor fraction to the second determination of tumor fraction, thereby monitoring the cancer progression or recurrence.
  • the gene panel may comprise at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 20, at least 30, at least 40, or more than 40 genes.
  • the disclosed methods may be used to generate a report of genomic and medical information associated with a patient by assessing genomic and medical information in at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 20, at least 30, at least 40, or more than 40 gene loci.
  • the disclosed methods may further comprise one or more of the steps of: (i) obtaining the sample from the subject (e.g., a subject suspected of having or determined to have cancer), (ii) extracting nucleic acid molecules (e.g., a mixture of tumor nucleic acid molecules and non-tumor nucleic acid molecules) from the sample, (iii) ligating one or more adapters to the nucleic acid molecules extracted from the sample (e.g., one or more amplification primers, flow cell adaptor sequences, substrate adapter sequences, or sample index sequences), (iv) performing a methylation conversion reaction to convert, e.g., non-methylated cytosine to uracil, (v) amplifying the nucleic acid molecules (e.g., using a polymerase chain reaction (PCR) amplification technique, a non-PCR amplification technique, or an isothermal amplification technique), (vi) capturing nucleic acid molecules from the amplified
  • PCR polymerase
  • the report comprises output from the methods described herein. In some instances, all or a portion of the report may be displayed in the graphical user interface of an online or web-based healthcare portal. In some instances, the report is transmitted via a computer network or peer-to-peer connection.
  • the disclosed methods may be used with any of a variety of samples.
  • the sample may comprise a tissue biopsy sample, a liquid biopsy sample, or a normal control.
  • the sample may be a liquid biopsy sample and may comprise blood, plasma, cerebrospinal fluid, sputum, stool, urine, or saliva.
  • the sample may be a liquid biopsy sample and may comprise circulating tumor cells (CTCs).
  • the sample may be a liquid biopsy sample and may comprise cell-free DNA (cfDNA), circulating tumor DNA (ctDNA), or any combination thereof.
  • the nucleic acid molecules extracted from a sample may comprise a mixture of tumor nucleic acid molecules and non-tumor nucleic acid molecules.
  • the tumor nucleic acid molecules may be derived from a tumor portion of a heterogeneous tissue biopsy sample, and the non-tumor nucleic acid molecules may be derived from a normal portion of the heterogeneous tissue biopsy sample.
  • the sample may comprise a liquid biopsy sample, and the tumor nucleic acid molecules may be derived from a circulating tumor DNA (ctDNA) fraction of the liquid biopsy sample while the non-tumor nucleic acid molecules may be derived from a non-tumor, cell-free DNA (cfDNA) fraction of the liquid biopsy sample.
  • ctDNA circulating tumor DNA
  • the disclosed methods for identifying fragmentomics based tumorspecific copy number alteration states in a liquid biopsy sample may be used to diagnose (or as part of a diagnosis of) the presence of disease or other condition (e.g., cancer, genetic disorders (such as Down Syndrome and Fragile X), neurological disorders, or any other disease type where detection of variants, e.g., copy number alternations, are relevant to diagnosing, treating, or predicting said disease) in a subject (e.g., a patient).
  • disease or other condition e.g., cancer, genetic disorders (such as Down Syndrome and Fragile X), neurological disorders, or any other disease type where detection of variants, e.g., copy number alternations, are relevant to diagnosing, treating, or predicting said disease
  • a subject e.g., a patient
  • the disclosed methods may be applicable to diagnosis of any of a variety of cancers as described elsewhere herein.
  • the disclosed methods for identifying fragmentomics based tumorspecific copy number alteration states in a liquid biopsy sample may be used to select a subject (e.g., a patient) for a clinical trial based on the clinically significant genomic and medical information value determined for one or more gene loci.
  • patient selection for clinical trials based on, e.g., identification of clinically significant genomic and medical information at one or more gene loci may accelerate the development of targeted therapies and improve the healthcare outcomes for treatment decisions.
  • the disclosed methods for identifying fragmentomics based tumorspecific copy number alteration states in a liquid biopsy sample may be used to select an appropriate therapy or treatment (e.g., an anti-cancer therapy or anti-cancer treatment) for a subject.
  • an appropriate therapy or treatment e.g., an anti-cancer therapy or anti-cancer treatment
  • the anti-cancer therapy or treatment may comprise use of a poly (ADP-ribose) polymerase inhibitor (PARPi), a platinum compound, chemotherapy, radiation therapy, a targeted therapy (e.g., immunotherapy), surgery, or any combination thereof.
  • PARPi poly (ADP-ribose) polymerase inhibitor
  • the disclosed methods for identifying fragmentomics based tumorspecific copy number alteration states in a liquid biopsy sample may be used in treating a disease (e.g., a cancer) in a subject.
  • a disease e.g., a cancer
  • an effective amount of an anti-cancer therapy or anti-cancer treatment may be administered to the subject.
  • the disclosed methods for identifying fragmentomics based tumorspecific copy number alteration states in a liquid biopsy sample may be used for monitoring disease progression or recurrence (e.g., cancer or tumor progression or recurrence) in a subject.
  • the methods may be used to determine clinically significant genomic and medical information in a first sample obtained from the subject at a first time point, and used to determine clinically significant genomic and medical information in a second sample obtained from the subject at a second time point, where comparison of the first determination of clinically significant genomic and medical information and the second determination of clinically significant genomic and medical information allows one to monitor disease progression or recurrence.
  • the first time point is chosen before the subject has been administered a therapy or treatment
  • the second time point is chosen after the subject has been administered the therapy or treatment.
  • the disclosed methods for identifying fragmentomics based tumorspecific copy number alteration states in a liquid biopsy sample may be used for adjusting a therapy or treatment (e.g., an anti-cancer treatment or anti-cancer therapy) for a subject, e.g., by adjusting a treatment dose and/or selecting a different treatment in response to a change in the determination of clinically significant genomic and medical information.
  • a therapy or treatment e.g., an anti-cancer treatment or anti-cancer therapy
  • the value of clinically significant genomic and medical information determined using the disclosed methods may be used as a prognostic or diagnostic indicator associated with the sample.
  • the prognostic or diagnostic indicator may comprise an indicator of the presence of a disease (e.g., cancer) in the sample, an indicator of the probability that a disease (e.g., cancer) is present in the sample, an indicator of the probability that the subject from which the sample was derived will develop a disease (e.g., cancer) (i.e., a risk factor), or an indicator of the likelihood that the subject from which the sample was derived will respond to a particular therapy or treatment.
  • the disclosed methods for identifying fragmentomics based tumorspecific copy number alterations in liquid biopsy may be implemented as part of a genomic profiling process that comprises identification of the presence of variant sequences at one or more gene loci in a sample derived from a subject as part of detecting, monitoring, predicting a risk factor, or selecting a treatment for a particular disease, e.g., cancer.
  • the variant panel selected for genomic profiling may comprise the detection of variant sequences at a selected set of gene loci.
  • the variant panel selected for genomic profiling may comprise detection of variant sequences at a number of gene loci through comprehensive genomic profiling (CGP), a next-generation sequencing (NGS) approach used to assess hundreds of genes (including relevant cancer biomarkers) in a single assay.
  • CGP comprehensive genomic profiling
  • NGS next-generation sequencing
  • Inclusion of the disclosed methods for generating a report of genomic and medical information associated with a patient as part of a genomic profiling process (or inclusion of the output from the disclosed methods for generating a report of genomic and medical information associated with a patient as part of the genomic profile of the subject) can improve the validity of, e.g., disease detection calls and treatment decisions, made on the basis of the genomic profile by, for example, independently confirming the presence of clinically significant genomic and medical information in a given patient sample.
  • a genomic profile may comprise information on the presence of genes (or variant sequences thereof), copy number variations, epigenetic traits, proteins (or modifications thereof), and/or other biomarkers in an individual’s genome and/or proteome, as well as information on the individual’s corresponding phenotypic traits and the interaction between genetic or genomic traits, phenotypic traits, and environmental factors.
  • a genomic profile for the subject may comprise results from a comprehensive genomic profiling (CGP) test, a nucleic acid sequencing-based test, a gene expression profiling test, a cancer hotspot panel test, a DNA methylation test, a DNA fragmentation test, an RNA fragmentation test, or any combination thereof.
  • CGP genomic profiling
  • the method can further include administering or applying a treatment or therapy (e.g., an anti-cancer agent, anti-cancer treatment, or anti-cancer therapy) to the subject based on the generated genomic profile.
  • a treatment or therapy e.g., an anti-cancer agent, anti-cancer treatment, or anti-cancer therapy
  • An anti-cancer agent or anti-cancer treatment may refer to a compound that is effective in the treatment of cancer cells.
  • anti-cancer agents or anti-cancer therapies include, but not limited to, alkylating agents, antimetabolites, natural products, hormones, chemotherapy, radiation therapy, immunotherapy, surgery, or a therapy configured to target a defect in a specific cell signaling pathway, e.g., a defect in a DNA mismatch repair (MMR) pathway.
  • MMR DNA mismatch repair
  • the disclosed methods and systems may be used with any of a variety of samples (also referred to herein as specimens) comprising nucleic acids (e.g., DNA or RNA) that are collected from a subject (e.g., a patient).
  • samples also referred to herein as specimens
  • nucleic acids e.g., DNA or RNA
  • Examples include, but are not limited to, a tumor sample, a tissue sample, a biopsy sample, a blood sample (e.g., a peripheral whole blood sample), a blood plasma sample, a blood serum sample, a lymph sample, a saliva sample, a sputum sample, a urine sample, a gynecological fluid sample, a circulating tumor cell (CTC) sample, a cerebral spinal fluid (CSF) sample, a pericardial fluid sample, a pleural fluid sample, an ascites (peritoneal fluid) sample, a feces (or stool) sample, or other body fluid, secretion, and/or excretion sample (or cell sample derived therefrom).
  • the sample may be frozen sample or a formalin- fixed paraffin-embedded (FFPE) sample.
  • FFPE formalin- fixed paraffin-embedded
  • the sample may be collected by tissue resection (e.g., surgical resection), needle biopsy, bone marrow biopsy, bone marrow aspiration, skin biopsy, endoscopic biopsy, fine needle aspiration, oral swab, nasal swab, vaginal swab or a cytology smear, scrapings, washings or lavages (such as a ductal lavages or bronchoalveolar lavages), etc.
  • tissue resection e.g., surgical resection
  • needle biopsy e.g., bone marrow biopsy, bone marrow aspiration, skin biopsy, endoscopic biopsy, fine needle aspiration, oral swab, nasal swab, vaginal swab or a cytology smear
  • fine needle aspiration e.g., oral swab, nasal swab, vaginal swab or a cytology smear
  • scrapings
  • the sample is a liquid biopsy sample, and may comprise, e.g., whole blood, blood plasma, blood serum, urine, stool, sputum, saliva, or cerebrospinal fluid.
  • the sample may be a liquid biopsy sample and may comprise circulating tumor cells (CTCs).
  • the sample may be a liquid biopsy sample and may comprise cell- free DNA (cfDNA), circulating tumor DNA (ctDNA), or any combination thereof.
  • the sample may comprise one or more premalignant or malignant cells.
  • Premalignant refers to a cell or tissue that is not yet malignant but is poised to become malignant.
  • the sample may be acquired from a solid tumor, a soft tissue tumor, or a metastatic lesion.
  • the sample may be acquired from a hematologic malignancy or pre-malignancy.
  • the sample may comprise a tissue or cells from a surgical margin.
  • the sample may comprise tumor-infiltrating lymphocytes.
  • the sample may comprise one or more non- malignant cells.
  • the sample may be, or is part of, a primary tumor or a metastasis (e.g., a metastasis biopsy sample).
  • the sample may be obtained from a site (e.g., a tumor site) with the highest percentage of tumor (e.g., tumor cells) as compared to adjacent sites (e.g., sites adjacent to the tumor).
  • the sample may be obtained from a site (e.g., a tumor site) with the largest tumor focus (e.g., the largest number of tumor cells as visualized under a microscope) as compared to adjacent sites (e.g., sites adjacent to the tumor).
  • the disclosed methods may further comprise analyzing a primary control (e.g., a normal tissue sample). In some instances, the disclosed methods may further comprise determining if a primary control is available and, if so, isolating a control nucleic acid (e.g., DNA) from said primary control. In some instances, the sample may comprise any normal control (e.g., a normal adjacent tissue (NAT)) if no primary control is available. In some instances, the sample may be or may comprise histologically normal tissue. In some instances, the method includes evaluating a sample, e.g., a histologically normal sample (e.g., from a surgical tissue margin) using the methods described herein.
  • a primary control e.g., a normal tissue sample.
  • the disclosed methods may further comprise determining if a primary control is available and, if so, isolating a control nucleic acid (e.g., DNA) from said primary control.
  • the sample may comprise any normal control (e.g.,
  • the disclosed methods may further comprise acquiring a sub-sample enriched for non-tumor cells, e.g., by macro-dissecting non-tumor tissue from said NAT in a sample not accompanied by a primary control. In some instances, the disclosed methods may further comprise determining that no primary control and no NAT is available, and marking said sample for analysis without a matched control.
  • samples obtained from histologically normal tissues may still comprise a genetic alteration such as a variant sequence as described herein.
  • the methods may thus further comprise re-classifying a sample based on the presence of the detected genetic alteration.
  • multiple samples e.g., from different subjects are processed simultaneously.
  • tissue samples e.g., solid tissue samples, soft tissue samples, metastatic lesions, or liquid biopsy samples.
  • tissues include, but are not limited to, connective tissue, muscle tissue, nervous tissue, epithelial tissue, and blood.
  • Tissue samples may be collected from any of the organs within an animal or human body.
  • human organs include, but are not limited to, the brain, heart, lungs, liver, kidneys, pancreas, spleen, thyroid, mammary glands, uterus, prostate, large intestine, small intestine, bladder, bone, skin, etc.
  • the nucleic acids extracted from the sample may comprise deoxyribonucleic acid (DNA) molecules.
  • DNA DNA that may be suitable for analysis by the disclosed methods include, but are not limited to, genomic DNA or fragments thereof, mitochondrial DNA or fragments thereof, cell-free DNA (cfDNA), and circulating tumor DNA (ctDNA).
  • Cell-free DNA (cfDNA) is comprised of fragments of DNA that are released from normal and/or cancerous cells during apoptosis and necrosis, and circulate in the blood stream and/or accumulate in other bodily fluids.
  • Circulating tumor DNA ctDNA is comprised of fragments of DNA that are released from cancerous cells and tumors that circulate in the blood stream and/or accumulate in other bodily fluids.
  • DNA is extracted from nucleated cells from the sample.
  • a sample may have a low nucleated cellularity, e.g., when the sample is comprised mainly of erythrocytes, lesional cells that contain excessive cytoplasm, or tissue with fibrosis.
  • a sample with low nucleated cellularity may require more, e.g., greater, tissue volume for DNA extraction.
  • the nucleic acids extracted from the sample may comprise ribonucleic acid (RNA) molecules.
  • RNA ribonucleic acid
  • examples of RNA that may be suitable for analysis by the disclosed methods include, but are not limited to, total cellular RNA, total cellular RNA after depletion of certain abundant RNA sequences (e.g., ribosomal RNAs), cell-free RNA (cfRNA), messenger RNA (mRNA) or fragments thereof, the poly(A)-tailed mRNA fraction of the total RNA, ribosomal RNA (rRNA) or fragments thereof, transfer RNA (tRNA) or fragments thereof, and mitochondrial RNA or fragments thereof.
  • ribosomal RNAs e.g., ribosomal RNAs
  • cfRNA cell-free RNA
  • mRNA messenger RNA
  • rRNA transfer RNA
  • tRNA transfer RNA
  • RNA may be extracted from the sample and converted to complementary DNA (cDNA) using, e.g., a reverse transcription reaction.
  • cDNA complementary DNA
  • the cDNA is produced by random-primed cDNA synthesis methods.
  • the cDNA synthesis is initiated at the poly(A) tail of mature mRNAs by priming with oligo(dT)-containing oligonucleotides. Methods for depletion, poly(A) enrichment, and cDNA synthesis are well known to those of skill in the art.
  • the sample may comprise a tumor content, e.g., comprising tumor cells or tumor cell nuclei.
  • the sample may comprise a tumor content of at least 5- 50%, 10-40%, 15-25%, or 20-30% tumor cell nuclei.
  • the sample may comprise a tumor content of at least 5%, at least 10%, at least 20%, at least 30%, at least 40%, or at least 50% tumor cell nuclei.
  • the percent tumor cell nuclei is determined (e.g., calculated) by dividing the number of tumor cells in the sample by the total number of all cells within the sample that have nuclei.
  • a different tumor content calculation may be required due to the presence of hepatocytes having nuclei with twice, or more than twice, the DNA content of other, e.g., non-hepatocyte, somatic cell nuclei.
  • the sensitivity of detection of a genetic alteration e.g., a variant sequence, or a determination of, e.g., microsatellite instability, may depend on the tumor content of the sample. For example, a sample having a lower tumor content can result in lower sensitivity of detection for a given size sample.
  • the sample comprises nucleic acid (e.g., DNA, RNA (or a cDNA derived from the RNA), or both), e.g., from a tumor or from normal tissue.
  • nucleic acid e.g., DNA, RNA (or a cDNA derived from the RNA), or both
  • the sample may further comprise a non-nucleic acid component, e.g., cells, protein, carbohydrate, or lipid, e.g., from the tumor or normal tissue.
  • the sample is obtained (e.g., collected) from a subject (e.g., patient) with a condition or disease (e.g., a hyperproliferative disease or a non-cancer indication) or suspected of having the condition or disease.
  • a condition or disease e.g., a hyperproliferative disease or a non-cancer indication
  • the hyperproliferative disease is a cancer.
  • the cancer is a solid tumor or a metastatic form thereof.
  • the cancer is a hematological cancer, e.g. a leukemia or lymphoma.
  • the subject has a cancer or is at risk of having a cancer.
  • the subject has a genetic predisposition to a cancer (e.g., having a genetic mutation that increases his or her baseline risk for developing a cancer).
  • the subject has been exposed to an environmental perturbation (e.g., radiation or a chemical) that increases his or her risk for developing a cancer.
  • the subject is in need of being monitored for development of a cancer.
  • the subject is in need of being monitored for cancer progression or regression, e.g., after being treated with an anti-cancer therapy (or anti-cancer treatment).
  • the subject is in need of being monitored for relapse of cancer.
  • the subject is in need of being monitored for minimum residual disease (MRD).
  • the subject has been, or is being treated, for cancer.
  • the subject has not been treated with an anti-cancer therapy (or anti-cancer treatment).
  • the subject e.g., a patient
  • a post-targeted therapy sample e.g., specimen
  • the post-targeted therapy sample is a sample obtained after the completion of the targeted therapy.
  • the patient has not been previously treated with a targeted therapy.
  • the sample comprises a resection, e.g., an original resection, or a resection following recurrence (e.g., following a disease recurrence post-therapy).
  • a resection e.g., an original resection
  • a resection following recurrence e.g., following a disease recurrence post-therapy
  • the sample is acquired from a subject having a cancer.
  • exemplary cancers include, but are not limited to, B cell cancer (e.g., multiple myeloma), melanomas, breast cancer, lung cancer (such as non-small cell lung carcinoma or NSCLC), bronchus cancer, colorectal cancer, prostate cancer, pancreatic cancer, stomach cancer, ovarian cancer, urinary bladder cancer, brain or central nervous system cancer, peripheral nervous system cancer, esophageal cancer, cervical cancer, uterine or endometrial cancer, cancer of the oral cavity or pharynx, liver cancer, kidney cancer, testicular cancer, biliary tract cancer, small bowel or appendix cancer, salivary gland cancer, thyroid gland cancer, adrenal gland cancer, osteosarcoma, chondrosarcoma, cancer of hematological tissues, adenocarcinomas, inflammatory myofibroblastic tumors, gastrointestinal stromal tumor (GIST), colon cancer, multiple myeloma (MM),
  • B cell cancer
  • the cancer is a hematologic malignancy (or premaligancy).
  • a hematologic malignancy refers to a tumor of the hematopoietic or lymphoid tissues, e.g., a tumor that affects blood, bone marrow, or lymph nodes.
  • Exemplary hematologic malignancies include, but are not limited to, leukemia (e.g., acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), chronic myelogenous leukemia (CML), hairy cell leukemia, acute monocytic leukemia (AMoL), chronic myelomonocytic leukemia (CMML), juvenile myelomonocytic leukemia (JMML), or large granular lymphocytic leukemia), lymphoma (e.g., AIDS-related lymphoma, cutaneous T-cell lymphoma, Hodgkin lymphoma (e.g., classical Hodgkin lymphoma or nodular lymphocyte- predominant Hodgkin lymphoma), mycosis fungoides, non-Hodgkin lymphoma (e.g., B-cell non-Hodgkin lymphoma (e.g.
  • DNA or RNA may be extracted from tissue samples, biopsy samples, blood samples, or other bodily fluid samples using any of a variety of techniques known to those of skill in the art (see, e.g., Example 1 of International Patent Application Publication No. WO 2012/092426; Tan, et al. (2009), “DNA, RNA, and Protein Extraction: The Past and The Present”, J. Biomed. Biotech. 2009:574398; the technical literature for the Maxwell® 16 LEV Blood DNA Kit (Promega Corporation, Madison, WI); and the Maxwell 16 Buccal Swab LEV DNA Purification Kit Technical Manual (Promega Literature #TM333, January 1, 2011, Promega Corporation, Madison, WI)). Protocols for RNA isolation are disclosed in, e.g., the Maxwell® 16 Total RNA Purification Kit Technical Bulletin (Promega Literature #TB351, August 2009, Promega Corporation, Madison, WI).
  • a typical DNA extraction procedure for example, comprises (i) collection of the fluid sample, cell sample, or tissue sample from which DNA is to be extracted, (ii) disruption of cell membranes (i.e., cell lysis), if necessary, to release DNA and other cytoplasmic components, (iii) treatment of the fluid sample or lysed sample with a concentrated salt solution to precipitate proteins, lipids, and RNA, followed by centrifugation to separate out the precipitated proteins, lipids, and RNA, and (iv) purification of DNA from the supernatant to remove detergents, proteins, salts, or other reagents used during the cell membrane lysis step.
  • Disruption of cell membranes may be performed using a variety of mechanical shear (e.g., by passing through a French press or fine needle) or ultrasonic disruption techniques.
  • the cell lysis step often comprises the use of detergents and surfactants to solubilize lipids the cellular and nuclear membranes.
  • the lysis step may further comprise use of proteases to break down protein, and/or the use of an RNase for digestion of RNA in the sample.
  • Examples of suitable techniques for DNA purification include, but are not limited to, (i) precipitation in ice-cold ethanol or isopropanol, followed by centrifugation (precipitation of DNA may be enhanced by increasing ionic strength, e.g., by addition of sodium acetate), (ii) phenol-chloroform extraction, followed by centrifugation to separate the aqueous phase containing the nucleic acid from the organic phase containing denatured protein, and (iii) solid phase chromatography where the nucleic acids adsorb to the solid phase (e.g., silica or other) depending on the pH and salt concentration of the buffer.
  • the solid phase e.g., silica or other
  • cellular and histone proteins bound to the DNA may be removed either by adding a protease or by having precipitated the proteins with sodium or ammonium acetate, or through extraction with a phenol-chloroform mixture prior to a DNA precipitation step.
  • DNA may be extracted using any of a variety of suitable commercial DNA extraction and purification kits. Examples include, but are not limited to, the QIAamp (for isolation of genomic DNA from human samples) and DNAeasy (for isolation of genomic DNA from animal or plant samples) kits from Qiagen (Germantown, MD) or the Maxwell® and ReliaPrepTM series of kits from Promega (Madison, WI).
  • the sample may comprise a formalin-fixed (also known as formaldehyde-fixed, or paraformaldehyde-fixed), paraffin-embedded (FFPE) tissue preparation.
  • FFPE formalin-fixed
  • the FFPE sample may be a tissue sample embedded in a matrix, e.g., an FFPE block.
  • Methods to isolate nucleic acids (e.g., DNA) from formaldehyde- or paraformaldehyde-fixed, paraffin-embedded (FFPE) tissues are disclosed in, e.g., Cronin, el al., (2004) Am J Pathol.
  • the Maxwell® 16 FFPE Plus LEV DNA Purification Kit is used with the Maxwell® 16 Instrument for purification of genomic DNA from 1 to 10 pm sections of FFPE tissue. DNA is purified using silica-clad paramagnetic particles (PMPs), and eluted in low elution volume.
  • PMPs silica-clad paramagnetic particles
  • the E.Z.N.A.® FFPE DNA Kit uses a spin column and buffer system for isolation of genomic DNA.
  • QIAamp® DNA FFPE Tissue Kit uses QIAamp® DNA Micro technology for purification of genomic and mitochondrial DNA.
  • the disclosed methods may further comprise determining or acquiring a yield value for the nucleic acid extracted from the sample and comparing the determined value to a reference value. For example, if the determined or acquired value is less than the reference value, the nucleic acids may be amplified prior to proceeding with library construction.
  • the disclosed methods may further comprise determining or acquiring a value for the size (or average size) of nucleic acid fragments in the sample, and comparing the determined or acquired value to a reference value, e.g., a size (or average size) of at least 100, 200, 300, 400, 500, 600, 700, 800, 400, or 500 base pairs (bps).
  • a reference value e.g., a size (or average size) of at least 100, 200, 300, 400, 500, 600, 700, 800, 400, or 500 base pairs (bps).
  • one or more parameters described herein may be adjusted or selected in response to this determination.
  • the nucleic acids are typically dissolved in a slightly alkaline buffer, e.g., Tris-EDTA (TE) buffer, or in ultra-pure water.
  • a slightly alkaline buffer e.g., Tris-EDTA (TE) buffer
  • the isolated nucleic acids may be fragmented or sheared by using any of a variety of techniques known to those of skill in the art.
  • genomic DNA can be fragmented by physical shearing methods, enzymatic cleavage methods, chemical cleavage methods, and other methods known to those of skill in the art. Methods for DNA shearing are described in Example 4 in International Patent Application Publication No. WO 2012/092426. In some instances, alternatives to DNA shearing methods can be used to avoid a ligation step during library preparation.
  • the nucleic acids isolated from the sample may be used to construct a library (e.g., a nucleic acid library as described herein).
  • the nucleic acids are fragmented using any of the methods described above, optionally subjected to repair of chain end damage, and optionally ligated to synthetic adapters, primers, and/or barcodes (e.g., amplification primers, sequencing adapters, flow cell adapters, substrate adapters, sample barcodes or indexes, and/or unique molecular identifier sequences), size-selected (e.g., by preparative gel electrophoresis), and/or amplified (e.g., using PCR, a non-PCR amplification technique, or an isothermal amplification technique).
  • synthetic adapters, primers, and/or barcodes e.g., amplification primers, sequencing adapters, flow cell adapters, substrate adapters, sample barcodes or indexes, and/or unique molecular identifier sequences
  • the fragmented and adapter-ligated group of nucleic acids is used without explicit size selection or amplification prior to hybridization-based selection of target sequences.
  • the nucleic acid is amplified by any of a variety of specific or nonspecific nucleic acid amplification methods known to those of skill in the art.
  • the nucleic acids are amplified, e.g., by a whole-genome amplification method such as random-primed strand-displacement amplification. Examples of nucleic acid library preparation techniques for next-generation sequencing are described in, e.g., van Dijk, et al. (2014), Exp. Cell Research 322: 12 - 20, and Illumina’s genomic DNA sample preparation kit.
  • the resulting nucleic acid library may contain all or substantially all of the complexity of the genome.
  • the term “substantially all” in this context refers to the possibility that there can in practice be some unwanted loss of genome complexity during the initial steps of the procedure.
  • the methods described herein also are useful in cases where the nucleic acid library comprises a portion of the genome, e.g., where the complexity of the genome is reduced by design. In some instances, any selected portion of the genome can be used with a method described herein. For example, in certain embodiments, the entire exome or a subset thereof is isolated.
  • the library may include at least 95%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, or 5% of the genomic DNA.
  • the library may consist of cDNA copies of genomic DNA that includes copies of at least 95%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, or 5% of the genomic DNA.
  • the amount of nucleic acid used to generate the nucleic acid library may be less than 5 micrograms, less than 1 microgram, less than 500 ng, less than 200 ng, less than 100 ng, less than 50 ng, less than 10 ng, less than 5 ng, or less than 1 ng.
  • a library e.g., a nucleic acid library
  • the nucleic acid molecules of the library can include a target nucleic acid molecule (e.g., a tumor nucleic acid molecule, a reference nucleic acid molecule and/or a control nucleic acid molecule; also referred to herein as a first, second and/or third nucleic acid molecule, respectively).
  • the nucleic acid molecules of the library can be from a single subject or individual.
  • a library can comprise nucleic acid molecules derived from more than one subject (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30 or more subjects).
  • two or more libraries from different subjects can be combined to form a library having nucleic acid molecules from more than one subject (where the nucleic acid molecules derived from each subject are optionally ligated to a unique sample barcode corresponding to a specific subject).
  • the subject is a human having, or at risk of having, a cancer or tumor.
  • the library may comprise one or more subgenomic intervals.
  • a subgenomic interval can be a single nucleotide position, e.g., a nucleotide position for which a variant at the position is associated (positively or negatively) with a tumor phenotype.
  • a subgenomic interval comprises more than one nucleotide position. Such instances include sequences of at least 2, 5, 10, 50, 100, 150, 250, or more than 250 nucleotide positions in length.
  • Subgenomic intervals can comprise, e.g., one or more entire genes (or portions thereof), one or more exons or coding sequences (or portions thereof), one or more introns (or portion thereof), one or more microsatellite region (or portions thereof), or any combination thereof.
  • a subgenomic interval can comprise all or a part of a fragment of a naturally occurring nucleic acid molecule, e.g., a genomic DNA molecule.
  • a subgenomic interval can correspond to a fragment of genomic DNA which is subjected to a sequencing reaction.
  • a subgenomic interval is a continuous sequence from a genomic source.
  • a subgenomic interval includes sequences that are not contiguous in the genome, e.g., subgenomic intervals in cDNA can include exonexonjunctions formed as a result of splicing.
  • the subgenomic interval comprises a tumor nucleic acid molecule.
  • the subgenomic interval comprises a non-tumor nucleic acid molecule.
  • the methods described herein can be used in combination with, or as part of, a method for evaluating a plurality or set of subject intervals (e.g., target sequences), e.g., from a set of genomic loci (e.g., gene loci or fragments thereof), as described herein.
  • a plurality or set of subject intervals e.g., target sequences
  • genomic loci e.g., gene loci or fragments thereof
  • the set of genomic loci evaluated by the disclosed methods comprises a plurality of, e.g., genes, which in mutant form, are associated with an effect on cell division, growth or survival, or are associated with a cancer, e.g., a cancer described herein.
  • the set of gene loci evaluated by the disclosed methods comprises at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, or more than 100 gene loci.
  • the selected gene loci may include subject intervals comprising non-coding sequences, coding sequences, intragenic regions, or intergenic regions of the subject genome.
  • the subject intervals can include a non-coding sequence or fragment thereof (e.g., a promoter sequence, enhancer sequence, 5’ untranslated region (5’ UTR), 3’ untranslated region (3’ UTR), or a fragment thereof), a coding sequence of fragment thereof, an exon sequence or fragment thereof, an intron sequence or a fragment thereof.
  • the methods described herein may comprise contacting a nucleic acid library with a plurality of target capture reagents in order to select and capture a plurality of specific target sequences (e.g., gene sequences or fragments thereof) for analysis.
  • a target capture reagent i.e., a molecule which can bind to and thereby allow capture of a target molecule
  • a target capture reagent is used to select the subject intervals to be analyzed.
  • a target capture reagent can be a bait molecule, e.g., a nucleic acid molecule (e.g., a DNA molecule or RNA molecule) which can hybridize to (i.e., is complementary to) a target molecule, and thereby allows capture of the target nucleic acid.
  • the target capture reagent e.g., a bait molecule (or bait sequence)
  • the target nucleic acid is a genomic DNA molecule, an RNA molecule, a cDNA molecule derived from an RNA molecule, a microsatellite DNA sequence, and the like.
  • the target capture reagent is suitable for solution-phase hybridization to the target. In some instances, the target capture reagent is suitable for solid-phase hybridization to the target. In some instances, the target capture reagent is suitable for both solution-phase and solid-phase hybridization to the target.
  • the design and construction of target capture reagents is described in more detail in, e.g., International Patent Application Publication No. WO 2020/236941, the entire content of which is incorporated herein by reference.
  • the methods described herein provide for optimized sequencing of a large number of genomic loci (e.g., genes or gene products (e.g., mRNA), micro satellite loci, etc.) from samples (e.g., cancerous tissue specimens, liquid biopsy samples, and the like) from one or more subjects by the appropriate selection of target capture reagents to select the target nucleic acid molecules to be sequenced.
  • a target capture reagent may hybridize to a specific target locus, e.g., a specific target gene locus or fragment thereof.
  • a target capture reagent may hybridize to a specific group of target loci, e.g., a specific group of gene loci or fragments thereof.
  • a plurality of target capture reagents comprising a mix of target- specific and/or group- specific target capture reagents may be used.
  • the number of target capture reagents (e.g., bait molecules) in the plurality of target capture reagents (e.g., a bait set) contacted with a nucleic acid library to capture a plurality of target sequences for nucleic acid sequencing is greater than 10, greater than 50, greater than 100, greater than 200, greater than 300, greater than 400, greater than 500, greater than 600, greater than 700, greater than 800, greater than 400, greater than 1,000, greater than 1,250, greater than 1,500, greater than 1,750, greater than 2,000, greater than 3,000, greater than 4,000, greater than 5,000, greater than 10,000, greater than 25,000, or greater than 50,000.
  • the overall length of the target capture reagent sequence can be between about 70 nucleotides and 500 nucleotides.
  • the target capture reagent length is between about 100 and 300 nucleotides, 110 and 200 nucleotides, or 120 and 170 nucleotides, in length.
  • intermediate oligonucleotide lengths of about 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 300, 400, 500, 600, 700, 800, and 400 nucleotides in length can be used in the methods described herein.
  • each target capture reagent sequence can include: (i) a target-specific capture sequence (e.g., a gene locus or micro satellite locus-specific complementary sequence), (ii) an adapter, primer, barcode, and/or unique molecular identifier sequence, and (iii) universal tails on one or both ends.
  • a target-specific capture sequence e.g., a gene locus or micro satellite locus-specific complementary sequence
  • an adapter, primer, barcode, and/or unique molecular identifier sequence e.g., an adapter, primer, barcode, and/or unique molecular identifier sequence
  • universal tails e.g., universal tails on one or both ends.
  • target capture reagent can refer to the targetspecific target capture sequence or to the entire target capture reagent oligonucleotide including the target- specific target capture sequence.
  • the target-specific capture sequences in the target capture reagents are between about 40 nucleotides and 500 nucleotides in length. In some instances, the targetspecific capture sequence is between about 70 nucleotides and 300 nucleotides in length. In some instances, the target- specific sequence is between about 100 nucleotides and 200 nucleotides in length. In yet other instances, the target- specific sequence is between about 120 nucleotides and 170 nucleotides in length, typically 120 nucleotides in length.
  • target-specific sequences of about 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 300, 400, 500, 600, 700, 800, and 400 nucleotides in length, as well as target-specific sequences of lengths between the above-mentioned lengths.
  • the target capture reagent may be designed to select a subject interval containing one or more rearrangements, e.g., an intron containing a genomic rearrangement.
  • the target capture reagent is designed such that repetitive sequences are masked to increase the selection efficiency.
  • complementary target capture reagents can be designed to recognize the juncture sequence to increase the selection efficiency.
  • the disclosed methods may comprise the use of target capture reagents designed to capture two or more different target categories, each category having a different target capture reagent design strategy.
  • the hybridization-based capture methods and target capture reagent compositions disclosed herein may provide for the capture and homogeneous coverage of a set of target sequences, while minimizing coverage of genomic sequences outside of the targeted set of sequences.
  • the target sequences may include the entire exome of genomic DNA or a selected subset thereof.
  • the target sequences may include, e.g., a large chromosomal region (e.g., a whole chromosome arm).
  • the methods and compositions disclosed herein provide different target capture reagents for achieving different sequencing depths and patterns of coverage for complex sets of target nucleic acid sequences.
  • DNA molecules are used as target capture reagent sequences, although RNA molecules can also be used.
  • a DNA molecule target capture reagent can be single stranded DNA (ssDNA) or double- stranded DNA (dsDNA).
  • ssDNA single stranded DNA
  • dsDNA double- stranded DNA
  • an RNA- DNA duplex is more stable than a DNA-DNA duplex and therefore provides for potentially better capture of nucleic acids.
  • the disclosed methods comprise providing a selected set of nucleic acid molecules (e.g., a library catch) captured from one or more nucleic acid libraries.
  • the method may comprise: providing one or a plurality of nucleic acid libraries, each comprising a plurality of nucleic acid molecules (e.g., a plurality of target nucleic acid molecules and/or reference nucleic acid molecules) extracted from one or more samples from one or more subjects; contacting the one or a plurality of libraries (e.g., in a solution-based hybridization reaction) with one, two, three, four, five, or more than five pluralities of target capture reagents (e.g., oligonucleotide target capture reagents) to form a hybridization mixture comprising a plurality of target capture reagent/nucleic acid molecule hybrids; separating the plurality of target capture reagent/nucleic acid molecule hybrids from said hybridization mixture, e.g., by
  • the disclosed methods may further comprise amplifying the library catch (e.g., by performing PCR). In other instances, the library catch is not amplified.
  • the target capture reagents can be part of a kit which can optionally comprise instructions, standards, buffers or enzymes or other reagents.
  • the methods disclosed herein may include the step of contacting the library (e.g., the nucleic acid library) with a plurality of target capture reagents to provide a selected library target nucleic acid sequences (z.e., the library catch).
  • the contacting step can be effected in, e.g., solution-based hybridization.
  • the method includes repeating the hybridization step for one or more additional rounds of solution-based hybridization.
  • the method further includes subjecting the library catch to one or more additional rounds of solution-based hybridization with the same or a different collection of target capture reagents.
  • the contacting step is effected using a solid support, e.g., an array.
  • a solid support e.g., an array.
  • suitable solid supports for hybridization are described in, e.g., Albert, T.J. et al. (2007) Nat. Methods 4(11):903-5; Hodges, E. et al. (2007) Nat. Genet. 39(12): 1522-7; and Okou, D.T. et al. (2007) Nat. Methods 4(11 ):907-9, the contents of which are incorporated herein by reference in their entireties.
  • Hybridization methods that can be adapted for use in the methods herein are described in the art, e.g., as described in International Patent Application Publication No. WO 2012/092426. Methods for hybridizing target capture reagents to a plurality of target nucleic acids are described in more detail in, e.g., International Patent Application Publication No. WO 2020/236941, the entire content of which is incorporated herein by reference.
  • the methods and systems disclosed herein can be used in combination with, or as part of, a method or system for sequencing nucleic acids (e.g., a next-generation sequencing system) to generate a plurality of sequence reads that overlap one or more gene loci within a subgenomic interval in the sample and thereby determine, e.g., gene allele sequences at a plurality of gene loci.
  • a method or system for sequencing nucleic acids e.g., a next-generation sequencing system
  • next-generation sequencing may also be referred to as “massively parallel sequencing,” and refers to any sequencing method that determines the nucleotide sequence of either individual nucleic acid molecules (e.g., as in single molecule sequencing) or clonally expanded proxies for individual nucleic acid molecules in a high throughput fashion (e.g., wherein greater than 10 3 , 10 4 , 10 5 or more than 10 5 molecules are sequenced simultaneously).
  • next-generation sequencing methods are known in the art, and are described in, e.g., Metzker, M. (2010) Nature Biotechnology Reviews 11:31-46, which is incorporated herein by reference.
  • Other examples of sequencing methods suitable for use when implementing the methods and systems disclosed herein are described in, e.g., International Patent Application Publication No. WO 2012/092426.
  • the sequencing may comprise, for example, whole genome sequencing (WGS), whole exome sequencing, targeted sequencing, or direct sequencing.
  • GGS whole genome sequencing
  • sequencing may be performed using, e.g., Sanger sequencing.
  • the sequencing may comprise a paired-end sequencing technique that allows both ends of a fragment to be sequenced and generates high-quality, alignable sequence data for detection of, e.g., genomic rearrangements, repetitive sequence elements, gene fusions, and novel transcripts.
  • sequencing may comprise Illumina MiSeq sequencing.
  • sequencing may comprise Illumina HiSeq sequencing.
  • sequencing may comprise Illumina NovaSeq sequencing. Optimized methods for sequencing a large number of target genomic loci in nucleic acids extracted from a sample are described in more detail in, e.g., International Patent Application Publication No. WO 2020/236941, the entire content of which is incorporated herein by reference.
  • the disclosed methods comprise one or more of the steps of: (a) acquiring a library comprising a plurality of normal and/or tumor nucleic acid molecules from a sample; (b) simultaneously or sequentially contacting the library with one, two, three, four, five, or more than five pluralities of target capture reagents under conditions that allow hybridization of the target capture reagents to the target nucleic acid molecules, thereby providing a selected set of captured normal and/or tumor nucleic acid molecules (z.e., a library catch); (c) separating the selected subset of the nucleic acid molecules (e.g., the library catch) from the hybridization mixture, e.g., by contacting the hybridization mixture with a binding entity that allows for separation of the target capture reagent/nucleic acid molecule hybrids from the hybridization mixture, (d) sequencing the library catch to acquiring a plurality of reads (e.g., sequence reads) that overlap one or more subject intervals (e.g.
  • acquiring sequence reads for one or more subject intervals may comprise sequencing at least 1, at least 5, at least 10, at least 20, at least 30, at least 40, at least 50, at least 100, at least 150, at least 200, at least 250, at least 300, at least 350, at least 400, at least 450, at least 500, at least 550, at least 600, at least 650, at least 700, at least 750, at least 800, at least 850, at least 400, at least 450, at least 1,000, at least 1,250, at least 1,500, at least 1,750, at least 2,000, at least 2,250, at least 2,500, at least 2,750, at least 3,000, at least 3,500, at least 4,000, at least 4,500, or at least 5,000 loci, e.g., genomic loci, gene loci, microsatellite loci, etc.
  • acquiring a sequence read for one or more subject intervals may comprise sequencing a subject interval for any number of loci within the range described in this paragraph, e
  • acquiring a sequence read for one or more subject intervals comprises sequencing a subject interval with a sequencing method that provides a sequence read length (or average sequence read length) of at least 20 bases, at least 30 bases, at least 40 bases, at least 50 bases, at least 60 bases, at least 70 bases, at least 80 bases, at least 90 bases, at least 100 bases, at least 120 bases, at least 140 bases, at least 160 bases, at least 180 bases, at least 200 bases, at least 220 bases, at least 240 bases, at least 260 bases, at least 280 bases, at least 300 bases, at least 320 bases, at least 340 bases, at least 360 bases, at least 380 bases, or at least 400 bases.
  • a sequencing method that provides a sequence read length (or average sequence read length) of at least 20 bases, at least 30 bases, at least 40 bases, at least 50 bases, at least 60 bases, at least 70 bases, at least 80 bases, at least 90 bases, at least 100 bases, at least 120 bases, at least 140 bases, at least 160 bases, at least 180 bases, at
  • acquiring a sequence read for the one or more subject intervals may comprise sequencing a subject interval with a sequencing method that provides a sequence read length (or average sequence read length) of any number of bases within the range described in this paragraph, e.g., a sequence read length (or average sequence read length) of 56 bases.
  • acquiring a sequence read for one or more subject intervals may comprise sequencing with at least lOOx or more coverage (or depth) on average.
  • acquiring a sequence read for one or more subject intervals may comprise sequencing with at least lOOx, at least 150x, at least 200x, at least 250x, at least 500x, at least 750x, at least l,000x, at least 1,500 x, at least 2,000x, at least 2,500x, at least 3,000x, at least 3,500x, at least 4,000x, at least 4,500x, at least 5,000x, at least 5,500x, or at least 6,000x or more coverage (or depth) on average.
  • acquiring a sequence read for one or more subject intervals may comprise sequencing with an average coverage (or depth) having any value within the range of values described in this paragraph, e.g., at least 160x.
  • acquiring a read for the one or more subject intervals comprises sequencing with an average sequencing depth having any value ranging from at least lOOx to at least 6,000x for greater than about 90%, 92%, 94%, 95%, 96%, 97%, 98%, or 99% of the gene loci sequenced.
  • acquiring a read for the subject interval comprises sequencing with an average sequencing depth of at least 125x for at least 99% of the gene loci sequenced.
  • acquiring a read for the subject interval comprises sequencing with an average sequencing depth of at least 4,100x for at least 95% of the gene loci sequenced.
  • the relative abundance of a nucleic acid species in the library can be estimated by counting the relative number of occurrences of their cognate sequences (e.g., the number of sequence reads for a given cognate sequence) in the data generated by the sequencing experiment.
  • the disclosed methods and systems provide nucleotide sequences for a set of subject intervals (e.g., gene loci), as described herein.
  • the sequences are provided without using a method that includes a matched normal control (e.g., a wild-type control) and/or a matched tumor control (e.g., primary versus metastatic).
  • the level of sequencing depth as used herein refers to the number of reads (e.g., unique reads) obtained after detection and removal of duplicate reads (e.g., PCR duplicate reads).
  • duplicate reads are evaluated, e.g., to support detection of copy number alteration (CNAs).
  • Alignment is the process of matching a read with a location, e.g., a genomic location or locus.
  • NGS reads may be aligned to a known reference sequence (e.g., a wild-type sequence).
  • NGS reads may be assembled de novo. Methods of sequence alignment for NGS reads are described in, e.g., Trapnell, C. and Salzberg, S.L. Nature Biotech., 2009, 27:455-457. Examples of de novo sequence assemblies are described in, e.g., Warren R., et al., Bioinformatics, 2007, 23:500-501; Butler, J.
  • Misalignment e.g., the placement of base-pairs from a short read at incorrect locations in the genome
  • misalignment of reads due to sequence context can lead to reduction in sensitivity of mutation detection
  • sequence context e.g., the presence of repetitive sequence
  • Other examples of sequence context that may cause misalignment include short-tandem repeats, interspersed repeats, low complexity regions, insertions - deletions (indels), and paralogs.
  • misalignment may introduce artifactual reads of “mutated” alleles by placing reads of actual reference genome base sequences at the wrong location. Because mutation-calling algorithms for multigene analysis should be sensitive to even low-abundance mutations, sequence misalignments may increase false positive discovery rates and/or reduce specificity.
  • the methods and systems disclosed herein may integrate the use of multiple, individually-tuned, alignment methods or algorithms to optimize base-calling performance in sequencing methods, particularly in methods that rely on massively parallel sequencing of a large number of diverse genetic events at a large number of diverse genomic loci.
  • the disclosed methods and systems may comprise the use of one or more global alignment algorithms.
  • the disclosed methods and systems may comprise the use of one or more local alignment algorithms. Examples of alignment algorithms that may be used include, but are not limited to, the Burrows-Wheeler Alignment (BWA) software bundle (see, e.g., Li, et al.
  • BWA Burrows-Wheeler Alignment
  • the methods and systems disclosed herein may also comprise the use of a sequence assembly algorithm, e.g., the Arachne sequence assembly algorithm (see, e.g., Batzoglou, et al. (2002), “ARACHNE: A Whole-Genome Shotgun Assembler”, Genome Res. 12: 177-189).
  • a sequence assembly algorithm e.g., the Arachne sequence assembly algorithm (see, e.g., Batzoglou, et al. (2002), “ARACHNE: A Whole-Genome Shotgun Assembler”, Genome Res. 12: 177-189).
  • the alignment method used to analyze sequence reads is not individually customized or tuned for detection of different variants (e.g., point mutations, insertions, deletions, and the like) at different genomic loci.
  • different alignment methods are used to analyze reads that are individually customized or tuned for detection of at least a subset of the different variants detected at different genomic loci.
  • different alignment methods are used to analyze reads that are individually customized or tuned to detect each different variant at different genomic loci.
  • tuning can be a function of one or more of: (i) the genetic locus (e.g., gene loci, micro satellite locus, or other subject interval) being sequenced, (ii) the tumor type associated with the sample, (iii) the variant being sequenced, or (iv) a characteristic of the sample or the subject.
  • the selection or use of alignment conditions that are individually tuned to a number of specific subject intervals to be sequenced allows optimization of speed, sensitivity, and specificity.
  • the method is particularly effective when the alignment of reads for a relatively large number of diverse subject intervals are optimized.
  • the method includes the use of an alignment method optimized for rearrangements in combination with other alignment methods optimized for subject intervals not associated with rearrangements.
  • the methods disclosed herein allow for the rapid and efficient alignment of troublesome reads, e.g., a read having a rearrangement.
  • a read for a subject interval comprises a nucleotide position with a rearrangement, e.g., a translocation
  • the method can comprise using an alignment method that is appropriately tuned and that includes: (i) selecting a rearrangement reference sequence for alignment with a read, wherein said rearrangement reference sequence aligns with a rearrangement (in some instances, the reference sequence is not identical to the genomic rearrangement); and (ii) comparing, e.g., aligning, a read with said rearrangement reference sequence.
  • a method of analyzing a sample can comprise: (i) performing a comparison (e.g., an alignment comparison) of a read using a first set of parameters (e.g., using a first mapping algorithm, or by comparison with a first reference sequence), and determining if said read meets a first alignment criterion (e.g., the read can be aligned with said first reference sequence, e.g., with less than a specific number of mismatches); (ii) if said read fails to meet the first alignment criterion, performing a second alignment comparison using a second set of parameters, (e.g., using a second mapping algorithm, or by comparison with a second reference sequence); and (iii) optionally, determining if said read meets said second criterion (e.g., the read can be
  • the alignment of sequence reads in the disclosed methods may be combined with a mutation calling method as described elsewhere herein.
  • reduced sensitivity for detecting actual mutations may be addressed by evaluating the quality of alignments (manually or in an automated fashion) around expected mutation sites in the genes or genomic loci (e.g., gene loci) being analyzed.
  • the sites to be evaluated can be obtained from databases of the human genome (e.g., the HG19 human reference genome) or cancer mutations (e.g., COSMIC).
  • Regions that are identified as problematic can be remedied with the use of an algorithm selected to give better performance in the relevant sequence context, e.g., by alignment optimization (or re-alignment) using slower, but more accurate alignment algorithms such as Smith- Waterman alignment.
  • customized alignment approaches may be created by, e.g., adjustment of maximum difference mismatch penalty parameters for genes with a high likelihood of containing substitutions; adjusting specific mismatch penalty parameters based on specific mutation types that are common in certain tumor types e.g. C- T in melanoma); or adjusting specific mismatch penalty parameters based on specific mutation types that are common in certain sample types e.g. substitutions that are common in FFPE).
  • Reduced specificity (increased false positive rate) in the evaluated subject intervals due to misalignment can be assessed by manual or automated examination of all mutation calls in the sequencing data. Those regions found to be prone to spurious mutation calls due to misalignment can be subjected to alignment remedies as discussed above. In cases where no algorithmic remedy is found possible, “mutations” from the problem regions can be classified or screened out from the panel of targeted loci.
  • the methods may include the use of an alignment method optimized for aligning sequence reads for DNA that has been converted using, e.g., a bisulfite reaction, to convert unmethylated cytosine residues to uracil (which is interpreted as a thymine in sequencing results).
  • sequence reads may be aligned to two genomes in silico, e.g., converted and unconverted versions of the reference genome, using such alignment tools.
  • Methylation occurs primarily at CpG sites, but may also occur less frequently at non-CpG sites (e.g., CHG or CHH sites).
  • the sequence read data may be obtained using a nucleic acid sequencing method comprising the use of a bisulfite- or enzymatic-conversion reaction (e.g., during library preparation) to convert non-methylated cytosine to uracil (see, e.g., Li, el al. (2011), “DNA Methylation Detection: Bisulfite Genomic Sequencing Analysis”, Methods Mol. Biol. 791: 11-21).
  • sequence read data may be obtained using a nucleic acid sequencing method comprising the use of alternative chemical and/or enzymatic reactions (e.g., during library preparation) to convert non-methylated cytosine to uracil (or to convert methylated cytosine to dihydrouracil).
  • enzymatic deamination of non-methylated cytosine using APOBEC to form uracil can be performed using, e.g., the Enzymatic Methyl-seq Kit from New England BioLabs (Ipswich, MA) which uses prior treatment with ten-eleven translocation methylcytosine dioxygenase 2 (TET2) to oxidize 5-mC and 5-hmC, thereby providing greater protection of the methylated cytosine from deamination by APOBEC).
  • TET2 ten-eleven translocation methylcytosine dioxygenase 2
  • sequence read data may be obtained using a nucleic acid sequencing method comprising the use of Methylated DNA Immunoprecipitation (MeDIP).
  • MeDIP Methylated DNA Immunoprecipitation
  • Examples of alignment tools optimized for aligning sequence reads for converted DNA include, but are not limited to, NovoAlign (Novocraft Technologies, Selangor, Malaysia), and the Bismark tool (Krueger, et al. (2011), “Bismark: A Flexible Aligner and Methylation Caller for Bisulfite-Seq Applications”, Bioinformatics 27(11): 1571-1572).
  • Base calling refers to the raw output of a sequencing device, e.g., the determined sequence of nucleotides in an oligonucleotide molecule.
  • Mutation calling refers to the process of selecting a nucleotide value, e.g., A, G, T, or C, for a given nucleotide position being sequenced. Typically, the sequence reads (or base calling) for a position will provide more than one value, e.g., some reads will indicate a T and some will indicate a G.
  • Mutation calling is the process of assigning a correct nucleotide value, e.g., one of those values, to the sequence.
  • mutant calling it can be applied to assign a nucleotide value to any nucleotide position, e.g., positions corresponding to mutant alleles, wild-type alleles, alleles that have not been characterized as either mutant or wild-type, or to positions not characterized by variability.
  • the disclosed methods may comprise the use of customized or tuned mutation calling algorithms or parameters thereof to optimize performance when applied to sequencing data, particularly in methods that rely on massively parallel sequencing of a large number of diverse genetic events at a large number of diverse genomic loci (e.g., gene loci, micro satellite regions, etc.) in samples, e.g., samples from a subject having cancer. Optimization of mutation calling is described in the art, e.g., as set out in International Patent Application Publication No. WO 2012/092426.
  • Methods for mutation calling can include one or more of the following: making independent calls based on the information at each position in the reference sequence (e.g., examining the sequence reads; examining the base calls and quality scores; calculating the probability of observed bases and quality scores given a potential genotype; and assigning genotypes (e.g., using Bayes’ rule)); removing false positives (e.g., using depth thresholds to reject SNPs with read depth much lower or higher than expected; local realignment to remove false positives due to small indels); and performing linkage disequilibrium (LD)/imputation- based analysis to refine the calls.
  • making independent calls based on the information at each position in the reference sequence e.g., examining the sequence reads; examining the base calls and quality scores; calculating the probability of observed bases and quality scores given a potential genotype; and assigning genotypes (e.g., using Bayes’ rule)
  • removing false positives e.g., using depth thresholds to reject SNP
  • Equations used to calculate the genotype likelihood associated with a specific genotype and position are described in, e.g., Li, H. and Durbin, R. Bioinformatics, 2010; 26(5): 589-95.
  • the prior expectation for a particular mutation in a certain cancer type can be used when evaluating samples from that cancer type.
  • Such likelihood can be derived from public databases of cancer mutations, e.g., Catalogue of Somatic Mutation in Cancer (COSMIC), HGMD (Human Gene Mutation Database), The SNP Consortium, Breast Cancer Mutation Data Base (BIC), and Breast Cancer Gene Database (BCGD).
  • Methods for generating indel calls and individual-level genotype likelihoods include, e.g., the Dindel algorithm (Albers, C.A., et al., Genome Res. 2011;21(6):961-73).
  • the Bayesian EM algorithm can be used to analyze the reads, make initial indel calls, and generate genotype likelihoods for each candidate indel, followed by imputation of genotypes using, e.g., QCALL (Le S.Q. and Durbin R. Genome Res. 2011;21(6):952-60).
  • Parameters, such as prior expectations of observing the indel can be adjusted ⁇ e.g., increased or decreased), based on the size or location of the indels.
  • the mutation calling method used to analyze sequence reads is not individually customized or fine-tuned for detection of different mutations at different genomic loci.
  • different mutation calling methods are used that are individually customized or fine-tuned for at least a subset of the different mutations detected at different genomic loci.
  • different mutation calling methods are used that are individually customized or fine-tuned for each different mutant detected at each different genomic loci.
  • the customization or tuning can be based on one or more of the factors described herein, e.g., the type of cancer in a sample, the gene or locus in which the subject interval to be sequenced is located, or the variant to be sequenced. This selection or use of mutation calling methods individually customized or fine-tuned for a number of subject intervals to be sequenced allows for optimization of speed, sensitivity and specificity of mutation calling.
  • a nucleotide value is assigned for a nucleotide position in each of X unique subject intervals using a unique mutation calling method, and X is at least 2, at least 3, at least 4, at least 5, at least 10, at least 15, at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 200, at least 300, at least 400, at least 500, at least 500, at least 1500, at least 2000, at least 2500, at least 3000, at least 3500, at least 4000, at least 4500, at least 5000, or greater.
  • the calling methods can differ, and thereby be unique, e.g., by relying on different Bayesian prior values.
  • assigning said nucleotide value is a function of a value which is or represents the prior (e.g., literature) expectation of observing a read showing a variant, e.g., a mutation, at said nucleotide position in a tumor of type.
  • the method comprises assigning a nucleotide value (e.g., calling a mutation) for at least 10, 20, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 400, or 1,000 nucleotide positions, wherein each assignment is a function of a unique value (as opposed to the value for the other assignments) which is or represents the prior (e.g., literature) expectation of observing a read showing a variant, e.g., a mutation, at said nucleotide position in a tumor of type.
  • a nucleotide value e.g., calling a mutation
  • assigning said nucleotide value is a function of a set of values which represent the probabilities of observing a read showing said variant at said nucleotide position if the variant is present in the sample at a specified frequency (e.g., 1%, 5%, 10%, etc.) and/or if the variant is absent (e.g., observed in the reads due to base-calling error alone).
  • the mutation calling methods described herein can include the following: (a) acquiring, for a nucleotide position in each of said X subject intervals: (i) a first value which is or represents the prior (e.g., literature) expectation of observing a read showing a variant, e.g., a mutation, at said nucleotide position in a tumor of type X; and (ii) a second set of values which represent the probabilities of observing a read showing said variant at said nucleotide position if the variant is present in the sample at a frequency (e.g., 1%, 5%, 10%, etc.) and/or if the variant is absent (e.g., observed in the reads due to base-calling error alone); and (b) responsive to said values, assigning a nucleotide value (e.g., calling a mutation) from said reads for each of said nucleotide positions by weighing, e.g., by a Bay
  • the methods described herein may comprise the use of a methylation status calling method, e.g., to call the methylation status of the CpG sites based on the sequence reads and fragments (complementary pairs of forward and reverse sequence reads) derived from DNA that has been subjected to a chemical or enzymatic conversion reaction, e.g., to convert unmethylated cytosine residues to uracil (which is interpreted as a thymine in sequencing results).
  • a methylation status calling method include, but are not limited to, the Bismark tool (Krueger, et al.
  • the systems may comprise, e.g., one or more processors, and a memory unit communicatively coupled to the one or more processors and configured to store instructions that, when executed by the one or more processors, cause the system perform operations including receiving, at the one or more processors, genomic testing data associated with the patient; based on the genomic testing data, retrieving, at the one or more processors, medical information including one or more potential clinical treatments for the patient; determining, by the one or more processors, that the medical information has at least some clinical significance to the patient; based on at least a portion of the medical information having at least some clinical significance to the patient, generating, by the one or more processors, patient- specific medical data; determining, by the one or more processors, at least one specific position to dispose the medical information on the report based on the patient-specific medical data; and generating, by the one or more processors, the report
  • the disclosed systems may further comprise a sequencer, e.g., a next generation sequencer (also referred to as a massively parallel sequencer).
  • a next generation sequencer also referred to as a massively parallel sequencer.
  • next generation (or massively parallel) sequencing platforms include, but are not limited to, the Roche 454, Illumina Solexa, ABI-SOLiD, ION Torrent, or Pacific Bioscience sequencing platforms.
  • the disclosed systems may be used for generating a report of genomic and medical information associated with a patient in any of a variety of samples as described herein (e.g., a tissue sample, biopsy sample, hematological sample, or liquid biopsy sample derived from the subject).
  • samples e.g., a tissue sample, biopsy sample, hematological sample, or liquid biopsy sample derived from the subject.
  • the plurality of gene loci for which sequencing data is processed to determine clinically significant genomic and medical information may comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more than 10 gene loci.
  • the nucleic acid sequence data is acquired using a next generation sequencing technique (also referred to as a massively parallel sequencing technique) having a read-length of less than 400 bases, less than 300 bases, less than 200 bases, less than 150 bases, less than 100 bases, less than 90 bases, less than 80 bases, less than 70 bases, less than 60 bases, less than 50 bases, less than 40 bases, or less than 30 bases.
  • a next generation sequencing technique also referred to as a massively parallel sequencing technique having a read-length of less than 400 bases, less than 300 bases, less than 200 bases, less than 150 bases, less than 100 bases, less than 90 bases, less than 80 bases, less than 70 bases, less than 60 bases, less than 50 bases, less than 40 bases, or less than 30 bases.
  • the determination of clinically significant genomic and medical information is used to select, initiate, adjust, or terminate a treatment for cancer in the subject (e.g., a patient) from which the sample was derived, as described elsewhere herein.
  • the disclosed systems may further comprise sample processing and library preparation workstations, microplate-handling robotics, fluid dispensing systems, temperature control modules, environmental control chambers, additional data storage modules, data communication modules (e.g., Bluetooth®, WiFi, intranet, or internet communication hardware and associated software), display modules, one or more local and/or cloud-based software packages (e.g., instrument / system control software packages, sequencing data analysis software packages), etc., or any combination thereof.
  • the systems may comprise, or be part of, a computer system or computer network as described elsewhere herein.
  • FIG. 6 illustrates an example of one or more computing device(s) 600 that may be utilized for identifying fragmentomics based tumor- specific copy number alterations in liquid biopsy, in accordance with the disclosed embodiments.
  • the one or more computing device(s) 600 may perform one or more steps of one or more methods described or illustrated herein.
  • the one or more computing device(s) 600 provide functionality described or illustrated herein.
  • software running on the one or more computing device(s) 600 performs one or more steps of one or more methods described or illustrated herein, or provides functionality described or illustrated herein.
  • Certain embodiments include one or more portions of the one or more computing device(s) 600.
  • This disclosure contemplates any suitable number of computing systems 600.
  • This disclosure contemplates one or more computing device(s) 600 taking any suitable physical form.
  • one or more computing device(s) 600 may be an embedded computer system, a system-on-chip (SOC), a single-board computer system (SBC) (e.g., a computer-on-module (COM) or system-on-module (SOM)), a desktop computer system, a laptop or notebook computer system, an interactive kiosk, a mainframe, a mesh of computer systems, a mobile telephone, a personal digital assistant (PDA), a server, a tablet computer system, an augmented/virtual reality device, or a combination of two or more of these.
  • the one or more computing device(s) 600 may be unitary or distributed; span multiple locations; span multiple machines; span multiple data centers; or reside in a cloud, which may include one or more cloud components in one or more networks.
  • the one or more computing device(s) 600 may perform without substantial spatial or temporal limitation one or more steps of one or more methods described or illustrated herein.
  • the one or more computing device(s) 600 may perform, in real-time or in batch mode, one or more steps of one or more methods described or illustrated herein.
  • the one or more computing device(s) 600 may perform, at different times or at different locations, one or more steps of one or more methods described or illustrated herein, where appropriate.
  • the one or more computing device(s) 600 includes a processor 602, memory 604, database 606, an input/output (I/O) interface 608, a communication interface 610, and a bus 612.
  • processor 602 includes hardware for executing instructions, such as those making up a computer program.
  • processor 602 may retrieve (or fetch) the instructions from an internal register, an internal cache, memory 604, or database 606; decode and execute them; and then write one or more results to an internal register, an internal cache, memory 604, or database 606.
  • processor 602 may include one or more internal caches for data, instructions, or addresses. This disclosure contemplates processor 602 including any suitable number of any suitable internal caches, where appropriate.
  • processor 602 may include one or more instruction caches, one or more data caches, and one or more translation lookaside buffers (TLBs). Instructions in the instruction caches may be copies of instructions in memory 604 or database 606, and the instruction caches may speed up retrieval of those instructions by processor 602.
  • TLBs translation lookaside buffers
  • memory 604 includes main memory for storing instructions for processor 602 to execute or data for processor 602 to operate on.
  • the one or more computing device(s) 600 may load instructions from database 606 or another source (such as, for example, another one or more computing device(s) 600) to memory 604.
  • Processor 602 may then load the instructions from memory 604 to an internal register or internal cache.
  • processor 602 may retrieve the instructions from the internal register or internal cache and decode them.
  • processor 602 may write one or more results (which may be intermediate or final results) to the internal register or internal cache.
  • Processor 602 may then write one or more of those results to memory 604.
  • processor 602 executes only instructions in one or more internal registers, internal caches, or memory 604 (as opposed to database 606 or elsewhere) and operates only on data in one or more internal registers, internal caches, or memory 604 (as opposed to database 606 or elsewhere).
  • One or more memory buses (which may each include an address bus and a data bus) may couple processor 602 to memory 604.
  • Bus 612 may include one or more memory buses, as described below.
  • one or more memory management units reside between processor 602 and memory 604 and facilitate accesses to memory 604 requested by processor 602.
  • memory 604 includes random access memory (RAM). This RAM may be volatile memory, where appropriate.
  • this RAM may be dynamic RAM (DRAM) or static RAM (SRAM). Moreover, where appropriate, this RAM may be single-ported or multi-ported RAM. This disclosure contemplates any suitable RAM.
  • Memory 604 may include one or more memory devices 604, where appropriate. Although this disclosure describes and illustrates particular memory, this disclosure contemplates any suitable memory.
  • database 606 includes mass storage for data or instructions.
  • database 606 may include a hard disk drive (HDD), a floppy disk drive, flash memory, an optical disc, a magneto-optical disc, magnetic tape, or a Universal Serial Bus (USB) drive, or a combination of two or more of these.
  • Database 606 may include removable or non-removable (or fixed) media, where appropriate.
  • Database 606 may be internal or external to the one or more computing device(s) 600, where appropriate.
  • database 606 is non-volatile, solid-state memory.
  • database 606 includes read-only memory (ROM).
  • this ROM may be maskprogrammed ROM, programmable ROM (PROM), erasable PROM (EPROM), electrically erasable PROM (EEPROM), electrically alterable ROM (EAROM), flash memory, or a combination of two or more of these.
  • This disclosure contemplates mass database 606 taking any suitable physical form.
  • Database 606 may include one or more storage control units facilitating communication between processor 602 and database 606, where appropriate.
  • database 606 may include one or more databases 606.
  • RO interface 608 includes hardware, software, or both, providing one or more interfaces for communication between the one or more computing device(s) 600 and one or more RO devices.
  • the one or more computing device(s) 600 may include one or more of these RO devices, where appropriate.
  • One or more of these RO devices may enable communication between a person and the one or more computing device(s) 600.
  • an RO device may include a keyboard, keypad, microphone, monitor, mouse, printer, scanner, speaker, still camera, stylus, tablet, touch screen, trackball, video camera, another suitable I/O device, or a combination of two or more of these.
  • An I/O device may include one or more sensors.
  • I/O interface 608 may include one or more device or software drivers enabling processor 602 to drive one or more of these I/O devices.
  • I/O interface 608 may include one or more I/O interfaces 608, where appropriate.
  • communication interface 610 includes hardware, software, or both providing one or more interfaces for communication (such as, for example, packet-based communication) between the one or more computing device(s) 600 and one or more other computing device(s) 600 or one or more networks.
  • communication interface 610 may include a network interface controller (NIC) or network adapter for communicating with an Ethernet or other wire -based network or a wireless NIC (WNIC) or wireless adapter for communicating with a wireless network, such as a WI-FI network.
  • NIC network interface controller
  • WNIC wireless NIC
  • WI-FI network wireless network
  • the one or more computing device(s) 600 may communicate with an ad hoc network, a personal area network (PAN), a local area network (LAN), a wide area network (WAN), a metropolitan area network (MAN), one or more portions of the Internet, or a combination of two or more of these.
  • PAN personal area network
  • LAN local area network
  • WAN wide area network
  • MAN metropolitan area network
  • One or more portions of one or more of these networks may be wired or wireless.
  • the one or more computing device(s) 600 may communicate with a wireless PAN (WPAN) (such as, for example, a BLUETOOTH WPAN), a WI-FI network, a WLMAX network, a cellular telephone network (such as, for example, a Global System for Mobile Communications (GSM) network), other suitable wireless network, or a combination of two or more of these.
  • WPAN wireless PAN
  • the one or more computing device(s) 600 may include any suitable communication interface 610 for any of these networks, where appropriate.
  • Communication interface 610 may include one or more communication interfaces 610, where appropriate.
  • bus 612 includes hardware, software, or both coupling components of the one or more computing device(s) 600 to each other.
  • bus 612 may include an Accelerated Graphics Port (AGP) or other graphics bus, an Enhanced Industry Standard Architecture (EISA) bus, a front-side bus (FSB), a HYPERTRANSPORT (HT) interconnect, an Industry Standard Architecture (ISA) bus, an INFINIBAND interconnect, a low-pin-count (LPC) bus, a memory bus, a Micro Channel Architecture (MCA) bus, a Peripheral Component Interconnect (PCI) bus, a PCI-Express (PCIe) bus, a serial advanced technology attachment (SATA) bus, a Video Electronics Standards Association local (VLB) bus, another suitable bus, or a combination of two or more of these.
  • Bus 612 may include one or more buses 612, where appropriate.
  • a computer-readable non-transitory storage medium or media may include one or more semiconductor-based or other integrated circuits (ICs) (such, as for example, field- programmable gate arrays (FPGAs) or application-specific ICs (ASICs)), hard disk drives (HDDs), hybrid hard drives (HHDs), optical discs, optical disc drives (ODDs), magneto-optical discs, magneto-optical drives, floppy diskettes, floppy disk drives (FDDs), magnetic tapes, solid- state drives (SSDs), RAM-drives, SECURE DIGITAL cards or drives, any other suitable computer-readable non-transitory storage media, or any suitable combination of two or more of these, where appropriate.
  • ICs semiconductor-based or other integrated circuits
  • HDDs hard disk drives
  • HHDs hybrid hard drives
  • ODDs optical disc drives
  • magneto-optical discs magneto-optical drives
  • FDDs floppy diskettes
  • FDDs floppy disk drives
  • a method comprising: providing a plurality of nucleic acid molecules obtained from a liquid biopsy sample from a subject; ligating one or more adapters onto one or more nucleic acid molecules from the plurality of nucleic acid molecules; amplifying the one or more ligated nucleic acid molecules from the plurality of nucleic acid molecules; capturing amplified nucleic acid molecules from the amplified nucleic acid molecules; sequencing, by a sequencer, the captured nucleic acid molecules to obtain a plurality of sequence reads that represent the captured nucleic acid molecules; receiving, at one or more processors, sequence read data for the plurality of sequence reads; determining, using the one or more processors, a normalized sequencing coverage depth for each of the one or more genomic loci based on the sequence read data; determining, using the one or more processors, a variant allele fraction for each of the one or more genomic loci based on the sequence read data; determining, using the one or more processors, one or more nucleic acid fragmentomic metrics for each
  • nucleic acid fragmentomic metrics determined for each of the one or more genomic loci are identified as being clinically-relevant based on an analysis of patient cohort data that includes clinical data, tissue sample sequencing data, liquid biopsy sample sequencing data, or any combination thereof, for patients that have been diagnosed with a disease.
  • nucleic acid fragmentomic metric of the one or more nucleic acid fragmentomic metrics is identified as being clinically-relevant based on a comparison to a corresponding threshold that discriminates between clinically-relevant copy number alteration states and non-clinically-relevant copy number alteration states in the patient cohort data.
  • the cancer comprises acute lymphoblastic leukemia (Philadelphia chromosome positive), acute lymphoblastic leukemia (precursor B-cell), acute myeloid leukemia (FLT3+), acute myeloid leukemia (with an IDH2 mutation), anaplastic large cell lymphoma, basal cell carcinoma, B-cell chronic lymphocytic leukemia, bladder cancer, breast cancer (HER2 overexpressed/amplified), breast cancer (HER2+), breast cancer (HR+, HER2-), cervical cancer, cholangiocarcinoma, chronic lymphocytic leukemia, chronic lymphocytic leukemia (with 17p deletion), chronic myelogenous leukemia, chronic myelogenous leukemia (Philadelphia chromosome positive), classical Hodgkin lymphoma, colorectal cancer, colorectal cancer (dMMR/MSI-H), colorectal cancer (KRAS wild type), cryopyrin
  • the targeted anti-cancer therapy comprises abemaciclib (Verzenio), abiraterone acetate (Zytiga), acalabrutinib (Calquence), ado-trastuzumab emtansine (Kadcyla), afatinib dimaleate (Gilotrif), aldesleukin (Proleukin), alectinib (Alecensa), alemtuzumab (Campath), alitretinoin (Panretin), alpelisib (Piqray), amivantamab-vmjw (Rybrevant), anastrozole (Arimidex), apalutamide (Erleada), asciminib hydrochloride (Scemblix), atezolizumab (Tecentriq), avapritinib (Ayvakit), avelumab (Bavencio), axicabtagene
  • liquid biopsy sample comprises circulating tumor cells (CTCs).
  • liquid biopsy sample comprises cell- free DNA (cfDNA), circulating tumor DNA (ctDNA), or any combination thereof.
  • s tumor nucleic acid molecules are derived from a circulating tumor DNA (ctDNA) fraction of the liquid biopsy sample
  • non-tumor nucleic acid molecules are derived from a non-tumor, cell-free DNA (cfDNA) fraction of the liquid biopsy sample.
  • the one or more bait molecules comprise one or more nucleic acid molecules, each comprising a region that is complementary to a region of a captured nucleic acid molecule.
  • amplifying nucleic acid molecules comprises performing a polymerase chain reaction (PCR) amplification technique, a non-PCR amplification technique, or an isothermal amplification technique.
  • PCR polymerase chain reaction
  • the one or more gene loci comprises between 10 and 20 loci, between 10 and 40 loci, between 10 and 60 loci, between 10 and 80 loci, between 10 and 100 loci, between 10 and 150 loci, between 10 and 200 loci, between 10 and 250 loci, between 10 and 300 loci, between 10 and 350 loci, between 10 and 400 loci, between 10 and 450 loci, between 10 and 500 loci, between 20 and 40 loci, between 20 and 60 loci, between 20 and 80 loci, between 20 and 100 loci, between 20 and 150 loci, between 20 and 200 loci, between 20 and 250 loci, between 20 and 300 loci, between 20 and 350 loci, between 20 and 400 loci, between 20 and 500 loci, between 40 and 60 loci, between 40 and 80 loci, between 40 and 100 loci, between 40 and 150 loci, between 40 and 200 loci, between 40 and 250 loci, between 40 and 300 loci, between 40 and 350 loci, between 40 and 400 loci, between 40 and 500 loci, between 40 and 60 loci,
  • the one or more gene loci comprise ABL1, ACVR1B, AKT1, AKT2, AKT3, ALK, ALOX12B, AMER1, APC, AR, ARAF, ARFRP1, ARID1A, ASXL1, ATM, ATR, ATRX, AURKA, AURKB, AXIN1, AXL, BAP1, BARD1, BCL2, BCL2L1, BCL2L2, BCL6, BCOR, BCORL1, BCR, BRAF, BRCA1, BRCA2, BRD4, BRIP1, BTG1, BTG2, BTK, CALR, CARD11, CASP8, CBFB, CBL, CCND1, CCND2, CCND3, CCNE1, CD22, CD274, CD70, CD74, CD79A, CD79B, CDC73, CDH1, CDK12, CDK4, CDK6, CDK8, CDKN1A, CDKN1B, CDKN2A, CDKN2B, CDKN2C, CE
  • a method for determining copy number alteration state for one or more genomic loci based on a liquid biopsy sample from a subject comprising: receiving, at one or more processors, sequence read data for a plurality of sequence reads derived from the liquid biopsy sample from the subject; determining, using the one or more processors, a normalized sequencing coverage depth for each of the one or more genomic loci based on the sequence read data; determining, using the one or more processors, a variant allele fraction for each of the one or more genomic loci based on the sequence read data; determining, using the one or more processors, one or more nucleic acid fragmentomic metrics for each of the one or more genomic loci based on the sequence read data; inputting, using the one or more processors, the one or more nucleic acid fragmentomic metrics, the sequencing coverage depth, and the variant allele fraction determined for each of the one or more genomic loci into a copy number model trained to determine a copy number alteration state for each of the one or more genomic loci; and out
  • nucleic acid fragmentomic metric of the one or more nucleic acid fragmentomic metrics is identified as being clinically-relevant based on a comparison to a corresponding threshold that discriminates between clinically-relevant copy number alteration states and non-clinically-relevant copy number alteration states in the patient cohort data.
  • variant allele fraction determined for each of the one or more genomic loci comprises a variant allele fraction determined for a plurality of germline single nucleotide polymorphisms (SNPs) located within the one or more genomic loci.
  • SNPs germline single nucleotide polymorphisms
  • nucleic acid fragmentomic metrics comprise a median nucleic acid fragment length, a mean nucleic acid fragment length, a mode of nucleic acid fragment lengths, a weighted average of nucleic acid fragment lengths, a 25 th percentile of nucleic acid fragment lengths, a 75 th percentile of nucleic acid fragment lengths, a nucleic acid fragment start location, a nucleic acid fragment end location, a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a nucleic acid fragment end feature, or any combination thereof.
  • nucleic acid fragmentomic metrics comprise a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • nucleic acid fragmentomic metrics comprise a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is determined based on an analysis of sequence read data for a control sample.
  • control sample comprises a process matched control.
  • control sample comprises a panel-of-normals control.
  • nucleic acid fragmentomic metrics comprise a nucleic acid fragment end metric and a nucleic acid fragment end-adjacent sequence metric
  • nucleic acid fragment end metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at each end of the nucleic acid fragment
  • nucleic acid fragment end-adjacent sequence metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at points adjacent to each end of the nucleic acid fragment.
  • a method for determining a tumor fraction for a liquid biopsy sample derived from a subject comprising: receiving, at one or more processors, sequence read data for a plurality of sequence reads derived from the liquid biopsy sample; determining, using the one or more processors, a variant allele frequency for each of a plurality of genomic loci based on the sequence read data; determining, using the one or more processors, one or more nucleic acid fragmentomic metrics for each of the plurality of genomic loci based on the sequence read data; comparing, using the one or more processors, a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in the liquid biopsy sample to a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in a control sample; and determining, using the one or more processors, a tumor fraction for the liquid biopsy sample based on the variant allele frequencies determined for the plurality of genomic loci and the comparison of the distributions of the one or
  • nucleic acid fragmentomic metrics comprise a median nucleic acid fragment length, a mean nucleic acid fragment length, a mode of nucleic acid fragment lengths, a weighted average of nucleic acid fragment lengths, a 25 th percentile of nucleic acid fragment lengths, a 75 th percentile of nucleic acid fragment lengths, a nucleic acid fragment start location, a nucleic acid fragment end location, a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a nucleic acid fragment end motif or feature, nucleic acid fragment end-adjacent sequence motifs of features, or any combination thereof.
  • nucleic acid fragmentomic metrics comprise a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 nucleotides.
  • nucleic acid fragmentomic metrics comprise a percentage of nucleic acid fragments that are shorter than a first predetermined fragment length threshold, and wherein the first predetermined fragment length threshold is determined based on an analysis of sequence read data for a control sample.
  • control sample comprises a panel-of- normals control.
  • nucleic acid fragmentomic metrics comprise a nucleic acid fragment end metric and a nucleic acid fragment end-adjacent sequence metric
  • nucleic acid fragment end metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at each end of the nucleic acid fragment
  • nucleic acid fragment end-adjacent sequence metric comprises a percent GC content or DNA motif within nucleic acid segments comprising 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at points adjacent to each end of the nucleic acid fragment.
  • the comparison of the distributions of the one or more nucleic acid fragmentomic metrics for the liquid biopsy sample and the control sample comprises determining a shift in a median nucleic acid fragment length, a shift in a mean nucleic acid fragment length, a shift in a mode of nucleic acid fragment lengths, a shift in a weighted average of nucleic acid fragment lengths, a shift in a 25 th percentile of nucleic acid fragment lengths, a shift in a 75 th percentile of nucleic acid fragment lengths, a shift in a nucleic acid fragment start location, a shift in a nucleic acid fragment end location, a shift in a percentage of nucleic acid fragments that are shorter than a predetermined fragment length threshold, a shift in a nucleic acid fragment end metric, or any combination thereof.
  • a method for monitoring cancer progression or recurrence in a subject comprising: determining a first copy number alteration state for each of one or more genomic loci in a first liquid biopsy sample obtained from the subject at a first time point according to the method of any one of clauses 31 to 57; determining a second copy number alteration state for each of the one or more genomic loci in a second liquid biopsy sample obtained from the subject at a second time point; and comparing the first determination of copy number alteration state to the second determination of copy number alteration state for each of the one or more genomic loci, thereby monitoring the cancer progression or recurrence.
  • a method for monitoring cancer progression or recurrence in a subject comprising: determining a first tumor fraction in a first liquid biopsy sample obtained from the subject at a first time point according to the method of any one of clauses 58 to 72; and determining a second tumor fraction in a second liquid biopsy sample obtained from the subject at a second time point; and comparing the first determination of tumor fraction to the second determination of tumor fraction, thereby monitoring the cancer progression or recurrence.
  • the genomic profile for the subject further comprises results from a comprehensive genomic profiling (CGP) test, a gene expression profiling test, a cancer hotspot panel test, a DNA methylation test, a DNA fragmentation test, an RNA fragmentation test, or any combination thereof.
  • CGP genomic profiling
  • genomic profile for the subject further comprises results from a comprehensive genomic profiling (CGP) test, a gene expression profiling test, a cancer hotspot panel test, a DNA methylation test, a DNA fragmentation test, an RNA fragmentation test, or any combination thereof.
  • CGP genomic profiling
  • a system comprising: one or more processors; and a memory communicatively coupled to the one or more processors and configured to store instructions that, when executed by the one or more processors, cause the system to: receive sequence read data for a plurality of sequence reads derived from the liquid biopsy sample from the subject; determine a normalized sequencing coverage depth for each of the one or more genomic loci based on the sequence read data; determine a variant allele fraction for each of the one or more genomic loci based on the sequence read data; determine one or more nucleic acid fragmentomic metrics for each of the one or more genomic loci based on the sequence read data; input the one or more nucleic acid fragmentomic metrics, the sequencing coverage depth, and the variant allele fraction determined for each of the one or more genomic loci into a copy number model trained to determine a copy number alteration state for each of the one or more genomic loci; and output the copy number alteration state determined for each of the one or more genomic loci.
  • a system comprising: one or more processors; and a memory communicatively coupled to the one or more processors and configured to store instructions that, when executed by the one or more processors, cause the system to: receive sequence read data for a plurality of sequence reads derived from the liquid biopsy sample; determine a variant allele frequency for each of a plurality of genomic loci based on the sequence read data; determine one or more nucleic acid fragmentomic metrics for each of the plurality of genomic loci based on the sequence read data; compare a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in the liquid biopsy sample to a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in a control sample; and determine a tumor fraction for the liquid biopsy sample based on the variant allele frequencies determined for the plurality of genomic loci and the comparison of the distributions of the one or more nucleic acid fragmentomic metrics.
  • a non-transitory computer-readable storage medium storing one or more programs, the one or more programs comprising instructions, which when executed by one or more processors of a system, cause the system to: receive sequence read data for a plurality of sequence reads derived from the liquid biopsy sample from the subject; determine a normalized sequencing coverage depth for each of the one or more genomic loci based on the sequence read data; determine a variant allele fraction for each of the one or more genomic loci based on the sequence read data; determine one or more nucleic acid fragmentomic metrics for each of the one or more genomic loci based on the sequence read data; input the one or more nucleic acid fragmentomic metrics, the sequencing coverage depth, and the variant allele fraction determined for each of the one or more genomic loci into a machicopy number model trained to determine a copy number alteration state for each of the one or more genomic loci; and output the copy number alteration state determined for each of the one or more genomic loci.
  • a non-transitory computer-readable storage medium storing one or more programs, the one or more programs comprising instructions, which when executed by one or more processors of a system, cause the system to: receive sequence read data for a plurality of sequence reads derived from the liquid biopsy sample; determine a variant allele frequency for each of a plurality of genomic loci based on the sequence read data; determine one or more nucleic acid fragmentomic metrics for each of the plurality of genomic loci based on the sequence read data; compare a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in the liquid biopsy sample to a distribution of the one or more nucleic acid fragmentomic metrics for the plurality of genomic loci in a control sample; and determine a tumor fraction for the liquid biopsy sample based on the variant allele frequencies determined for the plurality of genomic loci and the comparison of the distributions of the one or more nucleic acid fragmentomic metrics.

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Abstract

L'invention concerne des méthodes d'identification basée sur la fragmentomique d'états de modification du nombre de copies spécifiques à une tumeur dans un échantillon de biopsie liquide. La méthode consiste en la réception de données de lecture de séquence pour un échantillon de biopsie liquide pris sur un sujet, la détermination d'une profondeur de couverture de séquençage normalisée, la détermination d'une fraction d'allèle variant, et la détermination d'une ou plusieurs métriques de fragmentomique d'acide nucléique pour chacun d'un ou de plusieurs locus génomiques sur la base des données de lecture de séquence. Le procédé consiste en outre en l'entrée desdites une ou plusieurs métriques de fragmentomique d'acide nucléique, de la profondeur de couverture de séquençage et de la fraction d'allèle variant dans un modèle de nombre de copies entraîné pour déterminer un état de modification de nombre de copies pour chacun desdits un ou plusieurs locus génomiques, puis à produire en sortie les états de modification de nombre de copies déterminés pour chacun desdits un ou plusieurs locus génomiques.
PCT/US2023/080494 2022-11-21 2023-11-20 Identification basée sur la fragmentomique d'états de modification du nombre de copies spécifiques à une tumeur dans une biopsie liquide Ceased WO2024112643A1 (fr)

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Citations (2)

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US20200370129A1 (en) * 2018-07-23 2020-11-26 Guardant Health, Inc. Methods and systems for adjusting tumor mutational burden by tumor fraction and coverage
WO2022046947A1 (fr) * 2020-08-25 2022-03-03 Guardant Health, Inc. Méthodes et systèmes pour prédire l'origine d'un variant

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CA3030038A1 (fr) * 2016-07-06 2018-01-11 Guardant Health, Inc. Procedes de profilage d'un fragmentome d'acides nucleiques sans cellule

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US20200370129A1 (en) * 2018-07-23 2020-11-26 Guardant Health, Inc. Methods and systems for adjusting tumor mutational burden by tumor fraction and coverage
WO2022046947A1 (fr) * 2020-08-25 2022-03-03 Guardant Health, Inc. Méthodes et systèmes pour prédire l'origine d'un variant

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