WO2024204178A1 - Composition herbicide et procédé de lutte contre les mauvaises herbes - Google Patents

Composition herbicide et procédé de lutte contre les mauvaises herbes Download PDF

Info

Publication number
WO2024204178A1
WO2024204178A1 PCT/JP2024/011918 JP2024011918W WO2024204178A1 WO 2024204178 A1 WO2024204178 A1 WO 2024204178A1 JP 2024011918 W JP2024011918 W JP 2024011918W WO 2024204178 A1 WO2024204178 A1 WO 2024204178A1
Authority
WO
WIPO (PCT)
Prior art keywords
compound
salt
weeds
trolamine salt
trolamine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2024/011918
Other languages
English (en)
Japanese (ja)
Inventor
義伸 神
由直 定
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sumitomo Chemical Co Ltd
Original Assignee
Sumitomo Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sumitomo Chemical Co Ltd filed Critical Sumitomo Chemical Co Ltd
Publication of WO2024204178A1 publication Critical patent/WO2024204178A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
    • A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
    • A01N39/04—Aryloxy-acetic acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/84—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P13/00—Herbicides; Algicides

Definitions

  • the present invention relates to a herbicide composition and a method for controlling weeds.
  • Non-Patent Document 1 herbicide compositions containing a certain compound and 2,4-D or its salt (triisopropanol ammonium salt or choline salt) are known (see Patent Documents 1 to 3).
  • the objective of the present invention is to provide a herbicide composition and a weed control method that have excellent weed control effects.
  • the present inventors have found that an excellent weed control effect can be achieved by using a specific compound in combination with a 2,4-D-trolamine salt.
  • the present invention includes the following aspects.
  • a herbicide composition comprising at least one compound selected from the following compound group X and a 2,4-D trolamine salt: Compound group X: flumioxazin, trifludimoxazin, saflufenacil, thiafenacil, flufenoximacil, and a compound represented by the following formula (II): [2] The herbicidal composition according to [1], wherein the weight ratio of at least one compound selected from compound group X to the acid equivalent of the 2,4-D trolamine salt is in the range of 1:1 to 1:100. [3] The herbicidal composition according to [1], wherein at least one compound selected from the compound group X is flumioxazin.
  • a method for controlling weeds comprising the step of applying at least one compound selected from the following compound group X and a 2,4-D trolamine salt, together or separately in any order: Compound group X: flumioxazin, trifludimoxazin, saflufenacil, thiafenacil, flufenoximacil, and a compound represented by the following formula (II): [18] The method according to [17], wherein the weight ratio of at least one compound selected from compound group X to the acid equivalent of the 2,4-D trolamine salt is in the range of 1:1 to 1:100.
  • the present invention makes it possible to control weeds with high effectiveness.
  • the herbicide composition of the present invention contains at least one compound selected from the compound group X (hereinafter referred to as compound X) and 2,4-D trolamine salt.
  • Flumioxazin is a compound described on page 541 of The Pesticide Manual Nineteenth Edition (hereinafter referred to as the Pesticide Manual) published by the British Crop Production Council (BCPC).
  • Trifludimoxazine is a compound described on page 1204 of the Pesticide Manual.
  • Saflufenacil is a compound described on page 1065 of the Pesticide Manual.
  • Thiafenacil is a compound described on page 1164 of the Pesticide Manual.
  • Flufenoximacil is a compound described in WO2021/139482.
  • the compound represented by the formula (I) (hereinafter sometimes referred to as compound 1) is a compound described in WO2016/095768.
  • the compound represented by the formula (II) (hereinafter sometimes referred to as compound 2) is a compound described in WO2017/202768.
  • 2,4-D trolamine salt is a salt of 2,4-D described on page 304 of the Pesticide Manual, and is the compound described as "2,4-D-trolamine” on page 305 of the Pesticide Manual. Both of these compounds can be manufactured by known methods.
  • the composition of the present invention is usually a formulation prepared by mixing Compound X and 2,4-D trolamine salt with a carrier such as a solid carrier or a liquid carrier, and adding formulation auxiliary agents such as surfactants as necessary.
  • Preferred dosage forms of such formulations are aqueous suspension concentrates, oil dispersions, wettable powders, water dispersible granules, granules, aqueous emulsions, oil-in-water emulsions, and emulsifiable concentrates.
  • the total content of compound X and 2,4-D trolamine salt in the composition of the present invention is usually in the range of 0.01 to 99% by weight, preferably 1 to 80% by weight.
  • the amount of 2,4-D trolamine salt is expressed in acid equivalent. That is, in this specification, the amount of 2,4-D trolamine salt is the amount of 2,4-D (2,4-dichlorophenoxyacetic acid).
  • the weight ratio of compound X to 2,4-D trolamine salt in the composition of the present invention is typically in the range of 1:1 to 1:100, and may be, for example, in the range of 1:1 to 1:50, 1:1 to 1:20, 1:5 to 1:100, 1:5 to 1:50, 1:5 to 1:20, 1:10 to 1:100, 1:10 to 1:50, 1:15 to 1:100 or 1:15 to 1:50.
  • Preferred examples of the weight ratio of compound X to 2,4-D trolamine salt in the composition of the present invention include 1:1, 1:1.2, 1:1.5, 1:1.7, 1:2, 1:2.5, 1:3, 1:4, 1:5, 1:7, 1:10, 1:15, 1:20, 1:30, 1:40, 1:50, 1:75, and 1:100.
  • compound X in the composition of the present invention is flumioxazin
  • the weight ratio of flumioxazin to 2,4-D-trolamine salt in the composition of the present invention is typically in the range of 1:5 to 1:20.
  • Preferred examples of weight ratios of flumioxazin to 2,4-D trolamine salt in the composition of the present invention include 1:5, 1:7, 1:10, 1:15, and 1:20.
  • the weight ratio of trifludimoxadine to 2,4-D-trolamine salt in the composition of the present invention is usually in the range of 1:15 to 1:100.
  • Preferred examples of the weight ratio of trifludimoxazine to 2,4-D-trolamine salt in the composition of the present invention include 1:15, 1:20, 1:30, 1:40, 1:50, 1:75, and 1:100.
  • the weight ratio of saflufenacil to 2,4-D-trolamine salt in the composition of the present invention is usually in the range of 1:15 to 1:50.
  • weight ratios of saflufenacil to 2,4-D-trolamine salt in the composition of the present invention include 1:15, 1:20, 1:30, 1:40, and 1:50.
  • the weight ratio of thiafenacil to 2,4-D-trolamine salt in the composition of the present invention is typically in the range of 1:10 to 1:50.
  • weight ratios of thiafenacil to 2,4-D-trolamine salt in the composition of the present invention include 1:10, 1:15, 1:20, 1:30, 1:40, and 1:50.
  • compound X in the composition of the present invention is flufenoximacil
  • the weight ratio of flufenoximacil to 2,4-D-trolamine salt in the composition of the present invention is usually in the range of 1:10 to 1:50.
  • Preferred examples of the weight ratio of flufenoximacil to 2,4-D-trolamine salt in the composition of the present invention include 1:10, 1:15, 1:20, 1:30, 1:40, and 1:50.
  • the weight ratio of compound 1 to 2,4-D trolamine salt in the composition of the present invention is usually in the range of 1:10 to 1:50.
  • Preferred examples of the weight ratio of compound 1 to 2,4-D trolamine salt in the composition of the present invention include 1:10, 1:15, 1:20, 1:30, 1:40, and 1:50.
  • the weight ratio of compound 2 to 2,4-D trolamine salt in the composition of the present invention is usually in the range of 1:15 to 1:100.
  • Preferred examples of the weight ratio of compound 2 to 2,4-D trolamine salt in the composition of the present invention include 1:15, 1:20, 1:30, 1:40, 1:50, 1:75, and 1:100.
  • the composition of the present invention is used to control weeds.
  • the method of using the composition of the present invention is similar to the method of controlling weeds of the present invention described below.
  • the composition of the present invention exerts a more synergistic herbicidal effect on a wide range of weeds than would be expected from the herbicidal effects of compound X and 2,4-D trolamine salt when used alone, and can effectively control a wide range of weeds in crop and vegetable fields, orchards, and non-agricultural land where normal tillage and no-tillage cultivation is performed, without causing any problematic chemical damage to useful plants.
  • composition of the present invention may further contain other pesticide active ingredients, such as insecticides, nematicides and fungicides.
  • insecticides, nematicides and fungicides include neonicotinoid compounds, diamide compounds, carbamate compounds, organophosphorus compounds, biological nematicides and other insecticides, as well as azole compounds, strobilurin compounds, metalaxyl compounds, SDHI compounds, other fungicides and plant growth regulators.
  • the weed control method of the present invention comprises a step of applying compound X and a 2,4-D trolamine salt together or separately in any order (hereinafter referred to as the application step).
  • the application step is carried out in an agricultural crop field, a vegetable field, an orchard or a non-agricultural land, and in the application step, compound X and a 2,4-D trolamine salt are applied to a place where weeds are growing or will grow.
  • the composition of the present invention may be used, or a composition containing compound X and a composition containing a 2,4-D trolamine salt may be used in combination.
  • a composition containing compound X is applied first, and then a composition containing a 2,4-D trolamine salt is applied, or they are applied in the reverse order.
  • composition containing compound X and the composition containing 2,4-D trolamine salt are usually preparations prepared by mixing compound X and 2,4-D trolamine salt with a carrier such as a solid carrier and a liquid carrier, respectively, and adding formulation auxiliary agents such as surfactants as necessary.
  • Methods for applying compound X and 2,4-D trolamine salt include, for example, spraying compound X and 2,4-D trolamine salt on soil (soil treatment) and spraying on emerged weeds (foliage treatment).
  • Soil treatment and foliage treatment are usually carried out by mixing the composition of the present invention with water to obtain a spray solution, which is sprayed on soil or weeds using a sprayer.
  • the amount of spray solution is usually in the range of 50 to 1000 L/ha, and may be, for example, in the range of 100 to 500 L/ha or 140 to 300 L/ha.
  • composition containing compound X and a composition containing 2,4-D trolamine salt are each sprayed in accordance with the method for spraying the composition of the present invention.
  • the application rate of compound X and 2,4-D trolamine salt is usually in the range of 1 to 10,000 g/ha, as the total amount of compound X and 2,4-D trolamine salt, and may be, for example, in the range of 2 to 5,000 g/ha or 5 to 2,000 g/ha.
  • the weight ratio of compound X to 2,4-D trolamine salt in the method of the present invention is typically in the range of 1:1 to 1:100, and may be, for example, in the range of 1:1 to 1:50, 1:1 to 1:20, 1:5 to 1:100, 1:5 to 1:50, 1:5 to 1:20, 1:10 to 1:100, 1:10 to 1:50, 1:15 to 1:100 or 1:15 to 1:50.
  • Preferred examples of the weight ratio of compound X to 2,4-D trolamine salt in the method of the present invention include 1:1, 1:1.2, 1:1.5, 1:1.7, 1:2, 1:2.5, 1:3, 1:4, 1:5, 1:7, 1:10, 1:15, 1:20, 1:30, 1:40, 1:50, 1:75, and 1:100.
  • the weight ratio of flumioxazin to 2,4-D trolamine salt in the method of the present invention is typically in the range of 1:5 to 1:20.
  • the weight ratio of trifludimoxazine to 2,4-D-trolamine salt in the method of the present invention is typically in the range of 1:15 to 1:100.
  • Preferred examples of weight ratios of trifludimoxazine to 2,4-D-trolamine salt in the method of the present invention include 1:15, 1:20, 1:30, 1:40, 1:50, 1:75, and 1:100.
  • the weight ratio of saflufenacil to 2,4-D-trolamine salt in the method of the present invention is typically in the range of 1:15 to 1:50.
  • weight ratios of thiafenacil to 2,4-D-trolamine salt in the method of the present invention include 1:10, 1:15, 1:20, 1:30, 1:40, and 1:50.
  • compound X in the method of the present invention is flufenoximacil
  • the weight ratio of flufenoximacil to 2,4-D-trolamine salt in the method of the present invention is typically in the range of 1:10 to 1:50.
  • Preferred examples of weight ratios of flufenoximacil to 2,4-D-trolamine salt in the method of the present invention include 1:10, 1:15, 1:20, 1:30, 1:40, and 1:50.
  • compound X in the method of the present invention is compound 1
  • the weight ratio of compound 1 to 2,4-D trolamine salt in the method of the present invention is usually in the range of 1:10 to 1:50.
  • Preferred examples of the weight ratio of compound 1 to 2,4-D trolamine salt in the method of the present invention include 1:10, 1:15, 1:20, 1:30, 1:40, and 1:50.
  • the weight ratio of compound 2 to 2,4-D trolamine salt in the method of the present invention is usually in the range of 1:15 to 1:100.
  • Preferred examples of the weight ratio of compound 2 to 2,4-D trolamine salt in the method of the present invention include 1:15, 1:20, 1:30, 1:40, 1:50, 1:75, and 1:100.
  • the spray liquid sprayed in the method of the present invention may contain an adjuvant.
  • the type of adjuvant is not particularly limited, and when an oil-based adjuvant such as Agri-Dex or MSO (mineral oil such as paraffinic hydrocarbons, naphthenic hydrocarbons, aromatic hydrocarbons, or vegetable oil (soybean oil or rapeseed oil) esterified) is contained in the spray liquid, the adjuvant concentration in the spray liquid is 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, or 6% (volume/volume).
  • Agri-Dex or MSO mineral oil such as paraffinic hydrocarbons, naphthenic hydrocarbons, aromatic hydrocarbons, or vegetable oil (soybean oil or rapeseed oil) esterified
  • the adjuvant concentration in the spray liquid is 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, or 6% (volume/volume).
  • a nonionic adjuvant such as Induce (polyoxyalkylene alkyl ether, polyoxyalkylene fatty acid ester, alkylaryl alkoxylate, or alkylaryl polyoxyalkylene glycol)
  • the adjuvant concentration in the spray liquid is 0.05%, 0.1%, 0.25%, or 0.5% (volume/volume).
  • Other adjuvants include, for example, anionic adjuvants (substituted sulfonates) such as Gramin S, cationic adjuvants (polyoxyethyleneamines) such as Genamin T 200BM, and organosilicon adjuvants such as Silwet L 77.
  • the spray solution sprayed in the method of the present invention may further contain a drift reducing agent such as Intact (polyethylene glycol).
  • the pH and hardness of the spray solution prepared in the method of the present invention are not particularly limited, but the pH is usually in the range of 5 to 9, and the hardness is usually in the range of 0 to 500 ppm on the American hardness scale.
  • the time period during which compound X and 2,4-D trolamine salt are applied is not particularly limited, but is usually between 5:00 a.m. and 9:00 p.m., the photon flux density at the application site is usually in the range of 10 to 2500 micromoles/m2/sec, the temperature at the application site is usually in the range of 0 to 35 degrees Celsius, and the wind speed is usually 3 MPH or less.
  • the spray pressure when applying compound X and 2,4-D trolamine salt is not particularly limited, but is usually in the range of 30 to 120 PSI, preferably 40 to 80 PSI.
  • the vegetable fields where the application process is carried out include fields where Solanaceae vegetables (eggplant, tomato, green pepper, chilli, potato, etc.) are grown, fields where Cucurbitaceae vegetables (cucumber, pumpkin, zucchini, watermelon, melon, etc.) are grown, fields where Brassicaceae vegetables (radish, turnip, horseradish, kohlrabi, Chinese cabbage, cabbage, mustard, broccoli, cauliflower, etc.) are grown, fields where Asteraceae vegetables (burdock, chrysanthemum, artichoke, etc.) are grown, and fields where Asteraceae vegetables (burdock, chrysanthemum, artichoke, etc.) are grown.
  • fields for cultivating lily family vegetables include fields for cultivating lily family vegetables (leeks, onions, garlic, asparagus, etc.), fields for cultivating parsley family vegetables (carrots, parsley, celery, parsley, etc.), fields for cultivating chenopodiaceae vegetables (spinach, Swiss chard, etc.), fields for cultivating lamiaceae vegetables (perilla, mint, basil, lavender, etc.), strawberry fields, sweet potato fields, Chinese yam fields, taro fields, etc.
  • the orchards where the application process is carried out include orchards, tea gardens, mulberry gardens, coffee gardens, banana gardens, palm gardens, flower gardens, flower tree fields, nursery fields, nurseries, forests, gardens, etc.
  • Fruit trees include pome fruits (apples, European pears, Japanese pears, quince, quince, etc.), stone fruits (peaches, plums, nectarines, plums, cherries, apricots, prunes, etc.), citrus fruits (Satsuma mandarins, oranges, lemons, limes, grapefruits, etc.), nuts (chestnuts, walnuts, hazels, almonds, pistachios, cashew nuts, macadamia nuts, etc.), berries (grapes, blueberries, cranberries, blackberries, raspberries, etc.), persimmons, olives, loquats, etc.
  • Non-agricultural land where the application process is carried out includes sports fields, vacant lots, railroad tracks, parks, parking lots, roadsides, riverbeds, areas under power lines, residential areas, factory sites, etc.
  • the crops grown in the agricultural fields where the application process is carried out are not limited as long as they are varieties that are commonly grown as crops.
  • the above-mentioned plant varieties may be plants that can be produced by natural crossbreeding, plants that can arise through mutation, F1 hybrid plants, or transgenic plants (also called genetically modified plants). These plants generally have characteristics such as resistance to herbicides, accumulation of toxic substances against pests, suppression of susceptibility to diseases, increased yield potential, improved resistance to biotic and abiotic stress factors, accumulation of substances, and improved storage and processability.
  • An F1 hybrid plant is a first generation hybrid obtained by crossing two varieties of different lineages, and generally has hybrid vigor characteristics that are superior to those of either parent.
  • a transgenic plant is a plant obtained by introducing a foreign gene from another organism such as a microorganism, and has characteristics that cannot be easily obtained by cross breeding, mutagenesis, or natural recombination in a natural environment.
  • the techniques for producing the above-mentioned plants include, for example, conventional breeding techniques; recombinant gene technology; genomic breeding techniques; new breeding techniques; and genome editing techniques.
  • Conventional breeding techniques are techniques for obtaining plants with desired properties through mutation or crossbreeding.
  • Recombinant gene technology is a technique for giving new properties to a certain organism (e.g., a microorganism) by extracting a desired gene (DNA) from the organism and introducing it into the genome of another target organism, and an antisense technique or an RNA interference technique for giving new or improved characteristics by silencing other genes present in the plant.
  • Genomic breeding techniques are techniques for using genome information to make breeding more efficient, and include DNA marker (also called genome marker or gene marker) breeding techniques and genomic selection.
  • DNA marker breeding is a method for selecting progeny having a desired useful trait gene from a large number of progeny by using a DNA marker, which is a DNA sequence that marks the location of a specific useful trait gene on the genome.
  • a DNA marker which is a DNA sequence that marks the location of a specific useful trait gene on the genome.
  • Genomic selection is a method of creating a prediction formula from phenotype and genome information obtained in advance, and predicting characteristics from the prediction formula and genome information without evaluating the phenotype, which can contribute to the efficiency of breeding.
  • New breeding techniques are a general term for breeding techniques that combine molecular biological techniques.
  • Genome editing technology is a technology that converts genetic information in a sequence-specific manner, and allows for deletion of base sequences, replacement of amino acid sequences, introduction of foreign genes, etc.
  • such tools include zinc finger nucleases (ZFN), TALEN, CRISPR/Cas9, CRISPR/Cpf1, and Meganuclease, which are capable of sequence-specific DNA cleavage, as well as sequence-specific genome modification technologies such as CAS9 nickase and Target-AID, which are created by modifying the aforementioned tools.
  • ZFN zinc finger nucleases
  • TALEN zinc finger nucleases
  • CRISPR/Cas9 CRISPR/Cpf1
  • Meganuclease Meganuclease, which are capable of sequence-specific DNA cleavage, as well as sequence-specific genome modification technologies such as CAS9 nickase and Target-AID, which are created by modifying the aforementioned tools.
  • Examples of the above-mentioned plants include those listed in the GM APPROVAL DATABASE on the electronic information site (http://www.isaaa.org/) of the International Service for the Acquisition of Agri-Biotech Applications (ISAAA). More specifically, these include herbicide-resistant plants, pest-resistant plants, disease-resistant plants, plants with modified quality (e.g., increase or decrease in content or change in composition) of products (e.g., starch, amino acids, fatty acids, etc.), plants with modified fertility traits, abiotic stress-resistant plants, and plants with modified traits related to growth or yield.
  • modified quality e.g., increase or decrease in content or change in composition
  • products e.g., starch, amino acids, fatty acids, etc.
  • plants with modified fertility traits e.g., abiotic stress-resistant plants, and plants with modified traits related to growth or yield.
  • Plants that have been given herbicide resistance through genetic engineering technology also include plants that have been given resistance through genetic engineering technology to 4-hydroxyphenylpyruvate dioxygenase (hereinafter abbreviated as HPPD) inhibitors such as isoxaflutole, mesotrione, etc., acetolactate synthase (hereinafter abbreviated as ALS) inhibitors such as imidazolinone herbicides including imazethapyr and sulfonylurea herbicides including thifensulfuron methyl, 5-enolpyruvylshikimate-3-phosphate synthase (hereinafter abbreviated as EPSPS) inhibitors such as glyphosate, glutamine synthase inhibitors such as glufosinate, auxin-type herbicides such as 2,4-D, dicamba, etc., oxynil herbicides including bromoxynil, and protoporphyrinogen oxidase (hereinafter abbre
  • Preferred herbicide-resistant transgenic plants are cereals such as wheat, barley, rye, and oats, canola, sorghum, soybean, rice, rapeseed, sugar beet, sugarcane, grape, lentil, sunflower, alfalfa, pome fruits, stone fruits, coffee, tea, strawberry, turfgrass, tomato, potato, cucumber, lettuce, and other vegetables, more preferably cereals such as wheat, barley, rye, and oats, soybean, rice, vine, tomato, potato, and pome fruits.
  • Glyphosate herbicide-resistant plant obtained by introducing one or more of the glyphosate-resistant EPSPS gene (CP4 epsps) derived from Agrobacterium tumefaciens strain CP4, the glyphosate metabolic enzyme gene (gat4601, gat4621) derived from Bacillus licheniformis with enhanced metabolic activity of the glyphosate metabolic enzyme (glyphosate N-acetyltransferase) gene by shuffling technology, the glyphosate metabolic enzyme (glyphosate oxidase gene, goxv247) derived from Ochrobacterum anthropi strain LBAA, or the EPSPS gene (mepsps, 2mepsps) having a glyphosate-resistant mutation derived from maize.
  • CP4 epsps derived from Agrobacterium tumefaciens strain CP4
  • the glyphosate metabolic enzyme gene gat4601, gat4621
  • the main plants include alfalfa (Medicago sativa), Argentine canola (Brassica napus), cotton (Gossypium hirsutum L.), creeping bentgrass (Agrostis stolonifera), corn (Zea mays L.), Polish canola (Brassica rapa), potato (Solanum tuberosum L.), soybean (Glycine max L.), sugar beet (Beta vulgaris), and wheat (Triticum aestivum).
  • alfalfa Medicago sativa
  • Argentine canola Brassica napus
  • cotton Gossypium hirsutum L.
  • creeping bentgrass Agrostis stolonifera
  • corn Zea mays L.
  • Polish canola Brassica rapa
  • potato Solanum tuberosum L.
  • soybean Glycine max L.
  • sugar beet Beta vulgaris
  • wheat Tritic
  • genetically modified plants expressing glyphosate-resistant EPSPS derived from Agrobacterium are sold under trade names including "Roundup Ready (registered trademark)", genetically modified plants expressing glyphosate-metabolizing enzymes derived from Bacillus bacteria whose metabolic activity has been enhanced by shuffling technology are sold under trade names such as "Optimum (registered trademark) GAT (trademark)” and "Optimum (registered trademark) Gly canola”, and genetically modified plants expressing EPSPS having a glyphosate-resistant mutation derived from corn are sold under the trade name "GlyTol (trademark)”.
  • Glufosinate herbicide-resistant plants obtained by introducing one or more of the phosphinothricin N-acetyltransferase (PAT) gene (bar), a glufosinate-metabolizing enzyme derived from Streptomyces hygroscopicus, the phosphinothricin N-acetyltransferase (PAT) enzyme gene (pat), a glufosinate-metabolizing enzyme derived from Streptomyces viridochromogenes, or the synthetic pat gene (pat syn) derived from Streptomyces viridochromogenes strain Tu494.
  • PAT phosphinothricin N-acetyltransferase
  • the main plants include Argentine canola (Brassica napus), chicory (Cichorium intybus), cotton (Gossypium hirsutum L.), corn (Zea mays L.), Polish canola (Brassica rapa), rice (Oryza sativa L.), soybean (Glycine max L.), and sugar beet (Beta vulgaris).
  • Argentine canola Brainssica napus
  • chicory Ceichorium intybus
  • cotton Gossypium hirsutum L.
  • corn Zea mays L.
  • Polish canola Brassica rapa
  • rice Oryza sativa L.
  • soybean Glycine max L.
  • sugar beet Beta vulgaris
  • Transgenic plants derived from Streptomyces hygroscopicus containing glufosinate-metabolizing enzymes (bar) and Streptomyces viridochromogenes are sold under trade names including LibertyLinkTM, InVigorTM, and WideStrikeTM.
  • Oxynil herbicide (e.g. bromoxynil) resistant plants Transgenic plants that are resistant to oxynil herbicides, such as bromoxynil, are introduced with the nitrilase gene (bxn), an oxynil herbicide (e.g. bromoxynil) metabolizing enzyme derived from Klebsiella pneumoniae subsp. Ozaenae.
  • Major plants include Argentine canola (Brassica napus), cotton (Gossypium hirsutum L.), and tobacco (Nicotiana tabacum L.), and are sold under brand names including "Navigator(TM) canola" or "BXN(TM)".
  • ALS inhibitor-resistant plants Carnation (Dianthus caryophyllus) "Moondust(trademark)", “Moonshadow(trademark)”, “Moonshade(trademark)”, “Moonlite(trademark)”, “Moonaqua(trademark)”, “Moonvista(trademark)”, “Moonique(trademark)”, “Moonpearl(trademark)”, “Moonberry(trademark)”, “Moonvelvet(trademark)” into which the ALS gene (surB) derived from tobacco (Nicotiana tabacum) for resistance to ALS inhibitors has been introduced as a selection marker; Flax (Linum us poster) into which the ALS gene (als) derived from Arabidopsis thaliana for resistance to ALS inhibitors has been introduced These include corn (Zea mays L.) "CDC Triffid Flax”; corn (Zea mays L.) “Optimum(TM) GAT(TM)” that is resistant to sulfonylurea
  • HPPD inhibitor-resistant plants Soybeans that have both the HPPD gene (avhppd-03) for resistance to mesotrione derived from oat (Avena sativa) and the phosphinothricin N-acetyltransferase (PAT) gene (pat), a glufosinate-metabolizing enzyme derived from Streptomyces viridochromogenes, are sold under the brand name "Herbicide-tolerant Soybean line.”
  • 2,4-D resistant plants Corn introduced with the aryloxyalkanoate dioxygenase gene (aad-1), a 2,4-D metabolic enzyme derived from Sphingobium herbicidovorans, is sold under the brand name Enlist(TM) Maize. Soybeans and cotton introduced with the aryloxyalkanoate dioxygenase gene (aad-12), a 2,4-D metabolic enzyme derived from Delftia acidovorans, are sold under the brand name Enlist(TM) Soybean.
  • Dicamba-resistant plants Soybeans and cotton that have been introduced with the dicamba monooxygenase gene (dmo), a dicamba-metabolizing enzyme derived from Stenotrophomonas maltophilia strain DI-6.
  • Soybeans (Glycine max L.) that have been introduced with the glyphosate-resistant EPSPS gene (CP4 epsps) derived from Agrobacterium tumefaciens strain CP4 as well as the above genes are sold as "Genuity (registered trademark) Roundup Ready (trademark) 2 Xtend (trademark)".
  • Examples of PPO inhibitor-resistant plants include plants to which the ability to produce PPO with reduced affinity for PPO inhibitors has been imparted by genetic engineering technology, and plants to which the ability to detoxify and degrade PPO inhibitors by cytochrome P450 monooxygenase has been imparted by genetic engineering technology.
  • crops may be those to which both the ability to produce PPO with reduced affinity for PPO inhibitors and the ability to detoxify and degrade PPO inhibitors by cytochrome P450 monooxygenase have been imparted by genetic engineering technology.
  • Examples of commercially available transgenic plants that have been conferred herbicide tolerance include glyphosate-tolerant corn (Roundup Ready Corn), (Roundup Ready 2), (Agrisure GT), (Agrisure GT/CB/LL), (Agrisure GT/RW), (Agrisure 3000GT), (YieldGard VT Rootworm/RR2), and (YieldGard VT Triple); glyphosate-tolerant soybean (Roundup Ready Soybean) and (Optimum GAT); glyphosate-tolerant cotton (Roundup Ready Cotton) and (Roundup Ready Flex); glyphosate-tolerant canola (Roundup Ready Canola); glyphosate-tolerant These include: Roundup Ready Alfalfa, glyphosate-tolerant rice (Roundup Ready Rice); glufosinate-tolerant corn (Roundup Ready 2, Liberty Link, Herculex 1, Herculex RW, Herculex Xtra, Agrisure GT/CB/LL, Agri
  • soybean sugar beet, potato, tomato and tobacco with glufosinate tolerance (see, e.g., US6376754, US5646024, US5561236); cotton, peppers, apple, tomato, sunflower, tobacco, potato, corn, cucumber, wheat, soybean, sorghum and millet with 2,4-D tolerance (see, e.g., US6153401, US6100446, WO2005107437, US5608147 and US5670454); canola, corn, millet, barnyard millet, oats with ALS inhibitor (e.g., sulfonylurea and imidazolinone herbicides) tolerance.
  • ALS inhibitor e.g., sulfonylurea and imidazolinone herbicides
  • S653N, S654K, A122T, S653(At)N, S654(At) K, A122(At)T are known in rice and the like (see, for example, US2003/0217381, WO200520673); barley, sugarcane, rice, corn, tobacco, soybean, cotton, rapeseed, sugar beet, wheat and potato that are resistant to HPPD inhibitors (e.g., isoxazole herbicides such as isoxaflutole, triketone herbicides such as sulcotrione and mesotrione, pyrazole herbicides such as pyrazolinates, and diketonitrile decomposition products of isoxaflutole) (see, for example, WO2004/055191, WO199638567, WO1997049816 and US6791014).
  • HPPD inhibitors e.g., isoxazole herbicides such as isoxaflutole, triketone herbicide
  • Plants that have been conferred herbicide resistance through classical or genomic breeding techniques include, for example, Clearfield Rice, Clearfield Wheat, Clearfield Sunflower, Clearfield lentils, and Clearfield canola (BASF products) that are resistant to imidazolinone ALS inhibitors such as imazethapyr and imazamox; STS soybean that is resistant to sulfonylurea ALS inhibitors such as thifensulfuron-methyl; Examples include sethoxydim-resistant corn “SRcorn” and "Poast Protected(R) corn” which are resistant to acetyl-CoA carboxylase inhibitors such as oxime and aryloxyphenoxypropionic acid herbicides; sunflower “ExpressSun(R)” which is resistant to sulfonylurea herbicides such as tribenuron; rice “Provisia(TM) Rice” which is resistant to acetyl-CoA carboxylase inhibitors such as quizalofop; and canola "Tria
  • RTDS Rapid Trait Development System
  • GRON Gene Repair Oligonucleotide
  • Other examples include corn with herbicide resistance and reduced phytic acid content by deleting the endogenous gene IPK1 using zinc finger nuclease (see, for example, Nature 459, 437-441, 2009); and rice with herbicide resistance created using CRISPR-Cas9 (see, for example, Rice, 7, 5, 2014).
  • New breeding techniques that confer herbicide resistance include grafting, and an example of imparting the properties of a GM rootstock to a scion is the use of glyphosate-resistant Roundup Ready (registered trademark) soybean as a rootstock to confer glyphosate resistance to non-transgenic soybean scion (see Weed Technology 27:412-416, 2013).
  • the above-mentioned plants include lines that have been given two or more of the aforementioned abiotic stress resistance, disease resistance, herbicide resistance, pest resistance, growth or yield traits, nutrient uptake, product quality, fertility traits, etc., using recombinant gene technology, classical breeding technology, genomic breeding technology, new breeding technology, genome editing technology, etc., as well as lines that have been given two or more traits of parent lines by crossing plants with similar or different traits.
  • plants that have been made tolerant to two or more herbicides include, for example, glyphosate- and glufosinate-tolerant cotton (GlyTol(TM) LibertyLink(TM)), GlyTol(TM) LibertyLink(TM) ; glyphosate- and glufosinate-tolerant corn (Roundup Ready(TM) LibertyLink(TM) Maize); glufosinate- and 2,4-D-tolerant soybean (Enlist(TM) Soybean); glyphosate- and dicamba-tolerant soybean (Genuity(R) Roundup Ready(TM) 2 Xtend(TM) ); glyphosate- and ALS inhibitor-tolerant corn and soybean (OptimumGAT(TM) ); and genetically modified soybean (Enlist(TM) Soybean) that is tolerant to three herbicides, glyphosate, glufosinate, and 2,4-D.
  • GlyTol(TM) LibertyLink(TM) GlyTol(TM) LibertyLink(TM
  • FOPs aryloxyphenoxypropionic acids
  • Other genetically modified varieties that have been developed include cotton that is resistant to glufosinate and 2,4-D, cotton that is resistant to both glufosinate and dicamba, corn that is resistant to both glyphosate and 2,4-D, soybeans that are resistant to both glyphosate and HPPD herbicides, and genetically modified corn that is resistant to glyphosate, glufosinate, 2,4-D, aryloxyphenoxypropionic acid (FOPs) herbicides, and cyclohexadione (DIMs) herbicides.
  • FOPs aryloxyphenoxypropionic acid
  • DIMs cyclohexadione
  • YieldGard Roundup Ready and YieldGard Roundup Ready 2 corn which are tolerant to glyphosate and resistant to corn borer
  • Agrisure CB/LL corn which is tolerant to glufosinate and resistant to corn borer
  • Yield Gard VT Rootworm/RR2 corn which is tolerant to glyphosate and resistant to corn rootworm and corn borer
  • Yield Gard VT Triple corn which is tolerant to glyphosate and resistant to lepidopteran pests (Cry1F) (e.g., resistance to western bean cutworm, corn borer, black cutworm and fall armyworm);
  • Herculex I corn which is tolerant to glufosinate and resistant to lepidopteran pests (Cry1F) (e.g., resistance to western bean cutworm, corn borer, black cutworm and fall armyworm); Yield Gard VT Triple corn, which is tolerant to glyphosate and resistant to corn root
  • the nozzle used when applying compound X and 2,4-D trolamine salt in the method of the present invention may be a flat fan nozzle or a drift-reducing nozzle.
  • Flat fan nozzles include Teejet's Teejt110 series and XR Teejet110 series. When these are used at normal spray pressures, typically in the range of 30 to 120 PSI, the volume median diameter of droplets discharged from the nozzle is typically less than 430 microns.
  • a drift-reducing nozzle is a nozzle in which drift is reduced compared to a flat fan nozzle, and is called an air induction nozzle or a pre-orifice nozzle.
  • the volume median diameter of droplets discharged from a drift-reducing nozzle is typically 430 microns or more.
  • An air induction nozzle has an air introduction section between the nozzle inlet (chemical introduction section) and outlet (chemical discharge section), and forms droplets filled with air by mixing air into the chemical.
  • air induction nozzles include Green Leaf Technology's TDXL11003-D, TDXL11004-D1, TDXL11005-D1, and TDXL11006-D, Teejet's TTI110025, TTI11003, TTI11004, TTI11005, TTI11006, and TTI11008, and Pentair's ULD120-041, ULD120-051, and ULD120-061.
  • TTI11004 is particularly preferred.
  • a pre-orifice nozzle is a nozzle in which the inlet (chemical introduction part) is a metering orifice, which restricts the flow rate into the nozzle and reduces the pressure inside the nozzle, forming large droplets. This reduces the pressure at the time of discharge by roughly half compared to before introduction.
  • pre-orifice nozzles include Wilger's DR110-10, UR110-05, UR110-06, UR110-08, and UR110-10, and Teejet's 1/4TTJ08 Turf Jet and 1/4TTJ04 Turf Jet.
  • the sprayer used to apply compound X and 2,4-D-trolamine salt in the method of the present invention may be a hooded sprayer certified by the U.S. Environmental Protection Agency (EPA) for drift reduction technology (DRT).
  • hooded sprayers certified by DRT include REDBALL 642, REDBALL 642E, REDBALL SPK645, REDBALL 645, REDBALL 645T, REDBALL SP645, and REDBALL ATV642 from Willmar Fabrication LLC.
  • compound X and the 2,4-D trolamine salt may be applied before sowing the crops, or compound X and the 2,4-D trolamine salt may be applied simultaneously with and/or after sowing the crops. That is, the application frequency of compound X and 2,4-D trolamine salt is once before sowing, simultaneously with sowing, or once after sowing of the crop, twice excluding before sowing, twice excluding simultaneously with sowing, or twice excluding after sowing, or three times by applying at all of the timings before sowing, simultaneously with sowing, and after sowing.
  • compound X and the 2,4-D trolamine salt are usually applied from 50 days before sowing to just before sowing, preferably from 30 days before sowing to just before sowing, more preferably from 20 days before sowing to just before sowing, and even more preferably from 10 days before sowing to just before sowing.
  • compound X and 2,4-D trolamine salt are usually applied after sowing of a crop, compound X and 2,4-D trolamine salt are usually applied from immediately after sowing to before flowering, preferably from immediately after sowing to before emergence, or during the 1st to 6th true leaf stage of the crop.
  • a machine having a combination of a sower and a sprayer is used.
  • the method of applying compound X and 2,4-D trolamine salt includes soil treatment, in which compound X and 2,4-D trolamine salt are applied to soil where weeds grow, and foliage treatment, in which compound X and 2,4-D trolamine salt are applied to weeds that have grown and are growing.
  • the treatment can be both foliage treatment and soil treatment.
  • the application time can be determined independently of the above-mentioned growth stage of the crop. For example, foliage treatment can be performed on growing weeds before sowing the crop, or soil treatment can be performed while the crop is growing. These treatments can be uniformly applied to the land, or spot treatments.
  • spot treatment is a concept that is opposed to uniform, area-wide application of a herbicide, and means selective application of a herbicide to areas where weeds are growing or where weeds will occur.
  • “Apply to area” means application to the weeds or soil if weeds are growing, and application to the soil of areas where weeds are expected to occur in the future if weeds have not yet occurred.
  • a case in which compound X and 2,4-D trolamine salt are sprayed to an area where weeds have not occurred or will not occur in the future due to scattering, transpiration, etc., is also included in spot treatment, as long as it is not a uniform area-wide application.
  • spot treatment only a case in which all areas where weeds are growing or where weeds occur in a continuous cultivation area of a crop are selectively treated is not considered to be spot treatment. In other words, even if a part of the cultivation area is treated area-wide, or a part of the areas where weeds are growing or where weeds occur is not treated, it is included in spot treatment as long as there is a spot-treated area in a continuous cultivation area of a crop. Spot treatments can be performed while avoiding the crop, or can be performed based only on the location of the weeds, regardless of the location of the crop.
  • spot treatment methods are given below.
  • the applicator applies compound X and 2,4-D trolamine salt visually using a handheld nozzle or robot arm nozzle while walking or while riding on a ground-running or flying device.
  • the spot treatment may be performed by mapping the area where weeds grow or occur in advance and applying compound X and 2,4-D trolamine salt based on the map information.
  • spot treatment may be performed by automatically or manually opening and closing the nozzle on the boom or robot arm nozzle based on the position information of the applicator (obtained by GPS or the like) and the map information while the applicator is running or flying.
  • the map information may be created based on image information taken by a manned or unmanned flying object, or may be created visually by an observer walking on the ground, an observer riding on a ground-running device, or an observer riding on a flying device.
  • a running or flying sprayer may be equipped with a function for detecting where weeds are growing or occurring, and spot treatment may be performed using the boom or robot arm while performing real-time mapping.
  • Such technologies are described in patent literature (e.g., WO2018001893, WO2018036909) and non-patent literature (e.g., Crop Protection 26, 270-277, Weed Technology 17, 711-717, Applied Engineering in Agriculture. 30, 143-152).
  • the location of weed occurrence may be estimated based on the fact that the weed in question formed a vegetation patch in a past growing season, or it may be estimated from the distribution of buried seeds in the soil.
  • the distribution of buried seeds in the soil may be investigated by soil sampling or by remote sensing.
  • the method of the present invention exerts a more synergistic herbicidal effect against a wide range of weeds than would be expected from the herbicidal effects of compound X and 2,4-D-trolamine salt when used alone, and can effectively control a wide range of weeds in crop and vegetable fields, orchards, and non-agricultural land where normal tillage and no-tillage cultivation is practiced, without causing any problematic chemical damage to useful plants.
  • insecticide active ingredients such as insecticides, nematicides, and fungicides
  • insecticides, nematicides, and fungicides include neonicotinoid compounds, diamide compounds, carbamate compounds, organophosphorus compounds, biological nematicides, and other insecticides, as well as azole compounds, strobilurin compounds, metalaxyl compounds, SDHI compounds, other fungicides, and plant growth regulators.
  • Legume weeds (Fabaceae): Aeschynomene indica, Aeschynomene rudis, Sesbania exaltata, Cassia obtusifolia, Cassia occidentalis, Desmodium tortuosum, Desmodium adscendens, Desmodium illinoense, Trifolium repens, Pueraria lobata, Vicia angustifolia, Indigofera hirsuta, Indigofera truxillensis, Vigna sinensis Oxalidaceae: Wood sorrel (Oxalis corniculata), wood sorrel (Oxalis stricta), Oxalis oxyptera Geraniaceae weeds: Geranium carolinense, Erodium cicutarium Euphorbiaceae: Euphorbia helioscopia, Euphorbia maculata, Euphorbia humistrata, Euphorbia esula, Euphorbia heterophylla, Euphorbia bra
  • Malvaceae Abutilon theophrasti, Sida rhombifolia, Sida cordifolia, Sida spinosa, Sida glaziovii, Sida santaremnensis, Hibiscus trionum, Anoda cristata, Malvastrum coromandelianum Onagraceae: Ludwigia epilobioides, Ludwigia octovalvis, Ludwigia decurrens, Oenothera biennis, Oenothera laciniata Sterculiaceae: Waltheria indica Violaceae: Viola arvensis, Viola tricolor Cucurbitaceae: Sichyos angulatus, Echinocystis lobata, Momordica charantia Weeds of the Lythraceae family: Ammannia multiflora, Ammannia auriculata, Ammannia coccinea, Lythrum salicaria, Rotala indica E
  • Apiaceae Oenanthe javanica, Daucus carota, Conium maculatum Araliaceae: Hydrocotyle sibthorpioides, Hydrocotyle ranunculoides Ceratophyllaceae weeds: Ceratophyllum demersum Cabombaceae: Cabomba caroliniana Haloragaceae: Myriophyllum aquaticum, Myriophyllum verticillatum, watermilfoils (Myriophyllum spicatum, Myriophyllum heterophyllum, etc.) Sapindaceae weeds: Cardiospermum halicacabum Primulaceae weeds: Anagallis arvensis Asclepiadaceae: Giant milkweed (Asclepias syriaca), Honeyvine milkweed (Ampelamus albidus) Rubiaceae: Catchweed bedstraw (Galium aparine), Cleaver grass (Gal
  • Convolvulaceae Ipomoea nil, Ipomoea hederacea, Ipomoea purpurea, Ipomoea hederacea var. integriuscula, Ipomoea lacunosa, Ipomoea triloba, Ipomoea acuminata, Ipomoea hederifolia, Ipomoea coccinea, Ipomoea quamoclit, Ipomoea grandifolia, Ipomoea aristolochiaefolia, Ipomoea cairica, Convolvulus arvensis), Calystegia hederacea, Calystegia japonica, Merremia hederacea, Merremia aegyptia, Merremia cissoides, Jacquemontia tamnifolia Boraginaceae: Forget-me-not (Myosotis arvensis) Lam
  • Solanaceae Datura stramonium, Solanum nigrum, Solanum americanum, Solanum ptycanthum, Solanum sarrachoides, Solanum rostratum, Solanum aculeatissimum, Solanum sisymbriifolium, Solanum carolinense, Physalis angulata, Physalis subglabrata, Nicandra physalodes Scrophulariaceae: Veronica hederaefolia, Veronica persica, Veronica arvensis, Lindernia procumbens, Lindernia dubia, Lindernia angustifolia, Bacopa rotundifolia, Dopatrium junceum, Gratiola japonica Plantaginaceae: Plantago asiatica, Plantago lanceolata, Plantago major, and Callitriche palustris
  • Asteraceae weeds cocklebur (Xanthium pensylvanicum), cocklebur (Xanthium occidentale), burrbur (Xanthium italicum), wild sunflower (Helianthus annuus), chamomile (Matricaria chamomilla), chamomile (Matricaria perforata), corn marigold (Chrysanthemum segetum), orchid (Matricaria matricarioides), mugwort (Artemisia princeps), mugwort (Artemisia vulgaris), Chinese mugwort (Artemisia verlotorum), solidago altissima, dandelion (Taraxacum officinale), and hawkweed Galinsoga ciliata, Galinsoga parviflora, Senecio vulgaris, Senecio brasiliensis, Senecio grisebachii, Conyza bonariensis, Conyza smatrensis, Conyza canadens
  • Alismataceae weeds Sagittaria pygmaea, Sagittaria trifolia, Sagittaria sagittifolia, Sagittaria montevidensis, Sagittaria aginashi, Alisma canaliculatum, Alisma plantago-aquatica
  • Limnocharitaceae Limnocharis flava Hydrocharitaceae: Frogbit (Limnobium spongia), Hydrilla verticillata, Common water nymph (Najas guadalupensis)
  • Araceae weeds Pistia stratiotes Duckweeds (Lemnaceae): Lemna aoukikusa, Lemna paucicostata, Lemna aequinoctialis, Spirodela polyrhiza, Wolffia spp.
  • Potamogetonaceae Potamogeton distinctus, Pondweeds (Potamogeton crispus, Potamogeton illinoensis, Stuckenia pectinata, etc.)
  • Liliaceae Wild onion (Allium canadense), wild garlic (Allium vineale), nobile (Allium macrostemon)
  • Pontederiaceae Water hyacinth (Eichhornia crassipes), American whiteweed (Heteranthera limosa), Common water hyacinth (Monochoria korsakowii), and Common whiteweed (Monochoria vaginalis)
  • Poaceae weeds Barnyard grass (Echinochloa crus-galli), Barnyard grass (Echinochloa oryzicola), Barnyard grass (Echinochloa crus-galli var. formosensis), Latewater grass (Echinochloa oryzoides), Barnyard grass (Echinochloa colona), Gulf cockspur (Echinochloa crus-pavonis), Green foxtail (Setaria viridis), Autumn foxtail (Setaria faberi), Golden foxtail (Setaria glauca), American barnyard grass (Setaria viridis), Common foxtail (Setaria geniculata), crabgrass (Digitaria ciliaris), large crabgrass (Digitaria sanguinalis), Jamaican crabgrass (Digitaria horizontalis), broad crabgrass (Digitaria insularis), goosegrass (Eleusine indica), annual grass (Poa annua), common bluegrass (Poa trivialis), longgrass (
  • Cyperaceae weeds Cyperus microiria, Cyperus iria, Cyperus compressus, Cyperus difformis, Cyperus flaccidus, Cyperus globosus, Cyperus nipponicus, Cyperus odoratus, Cyperus serotinus, Cyperus rotundus, Cyperus esculentus, Kyllinga gracillima, Kyllinga brevifolia, Fimbristylis miliacea, Fimbristylis dichotoma), Eleocharis acicularis, Eleocharis kuroguwai, Schoenoplectiella hotarui, Schoenoplectiella juncoides, Schoenoplectiella wallichii, Schoenoplectiella mucronatus, Schoenoplectiella triangulatus, Schoenoplectiella nipponicus, Schoenoplectiella triqueter, Bolboschoenus
  • the intraspecific mutations of the above weeds are not particularly limited. In other words, they also include those that have reduced sensitivity (also called resistance) to a particular herbicide.
  • the reduced sensitivity may be due to a mutation at the target site (site of action mutation) or due to a factor other than the site of action (non-site of action mutation).
  • Site of action mutations include those in which an amino acid substitution occurs in the protein that is the target site due to a mutation in the nucleic acid sequence portion (open reading frame) that corresponds to the amino acid sequence of the protein, and those in which the protein at the target site is overexpressed due to a mutation such as deletion of a suppressor sequence in the promoter region, amplification of an enhancer sequence, or an increase in the copy number of a gene.
  • Factors that cause reduced susceptibility due to non-acting site mutations include increased metabolism, insufficient absorption, insufficient transport, and excretion from the system.
  • factors that cause increased metabolism include increased activity of metabolic enzymes such as cytochrome P450 monooxygenase (CYP), aryl acylamidase (AAA), esterase, and glutathione S-transferase (GST).
  • metabolic enzymes such as cytochrome P450 monooxygenase (CYP), aryl acylamidase (AAA), esterase, and glutathione S-transferase (GST).
  • Examples of excretion from the system include transport to the vacuole by ABC transporters.
  • Examples of herbicide resistant weeds include: Glyphosate Resistance: Examples of weed susceptibility reduction due to site mutations include weeds with one or more of the following amino acid substitutions in the EPSPS gene: Thr102Ile, Pro106Ser, Pro106Ala, Pro106Leu, Pro106Thr. In particular, weeds with both Thr102Ile and Pro106Ser, and those with both Thr102Ile and Pro106Thr, are included. Glyphosate-resistant goosegrass, Japanese barley, Japanese ryegrass, Bidens subalternans, etc. with these site mutations are effectively controlled.
  • glyphosate resistance involving the site of action is an increase in the copy number of the EPSPS gene (PNAS, 2018 115 (13) 3332-3337).
  • glyphosate-resistant weeds with an increased copy number of the EPSPS gene such as Amaranth, Waterhemp, and Kochia
  • Examples of weeds with reduced susceptibility due to non-acting point mutations include glyphosate-resistant Artemisia gracilis, Rhaematous sieboldii, and Rhaematous sieboldii, which are resistant to glyphosate due to the involvement of ABC transporters, and these can be effectively controlled by the present invention.
  • Echinochloa japonica is known to have reduced susceptibility to glyphosate due to increased expression of aldo-keto reductase (Plant Physiology 181, 1519-1534), and can be effectively controlled by the present invention.
  • ALS-inhibited herbicide resistance examples include weeds with mutations in the ALS gene that cause one or more of the following amino acid substitutions: Ala122Thr, Ala122Val, Ala122Tyr, Pro197Ser, Pro197His, Pro197Thr, Pro197Arg, Pro197Leu, Pro197Gln, Pro197Ala, Pro197Ile, Ala205Val, Ala205Phe, Asp376Glu, Asp376Gln, Asp376Asn, Arg377His, Trp574Leu, Trp574Gly, Trp574Met, Ser653Thr, Ser653Asn, Ser635Ile, Gly654Glu, and Gly645Asp.
  • ALS inhibitor-resistant weeds having these site-of-action mutations such as Amaranthus retroflexus, Amaranthus retroflexus, Waterhemp, and Kochia japonica, can be effectively controlled.
  • site-of-action mutations such as Amaranthus retroflexus, Amaranthus retroflexus, Waterhemp, and Kochia japonica
  • Examples of weeds with reduced susceptibility due to non-site-of-action mutations include weeds that have become resistant to ALS inhibitors due to the involvement of CYP or GST, and these weeds can be effectively controlled by the present invention.
  • weeds include Boumugi, which overexpresses CYP81A10 or CYP81A1v1, Echinochloa oryzicola, which overexpresses CYP81A12 or CYP81A21, and Black-legged foxtail, which overexpresses GSTF1 or GSTU2.
  • ACCase inhibitor resistance examples include weeds with mutations in the ACCase gene that cause one or more of the following amino acid substitutions: Ile1781Ala, Ile1781Leu, Ile1781Val, Ile1781Thr, Leu1818Phe, Trp1999Cys, Trp1999Leu, Trp1999Ser, Ala2004Val, Trp2027Cys, Trp2027Leu, Ile2041Asn, Ile2041Asp, Ile2041Val, Ile2041Thr, Asp2078Gly, Asp2078Glu, Cys2088Arg, Cys2088Phe, Gly2096Ala, and Gly2096Ser.
  • the present invention effectively controls ACCase-resistant weeds with these site mutations.
  • ACCase inhibitor resistance involving the site of action is increased expression of the ACCase gene (Pest Manag. Sci. 2017 Nov;73(11):2227-2235).
  • An example of reduced susceptibility of weeds due to non-site of action mutations is weeds that have become resistant to ACCase inhibitors due to the involvement of CYP or GST, and these can be effectively controlled by the present invention.
  • Examples of such weeds include Boumugi, which overexpresses CYP81A10 or CYP81A1v1, Echinochloa oryzicola, which overexpresses CYP81A12 or CYP81A21, and Black-legged foxtail, which overexpresses GSTF1 or GSTU2.
  • PPO inhibitor resistance Examples of reduced susceptibility of weeds due to site-of-effect mutations include weeds that have mutations in the PPO gene that result in one or more of the following amino acid substitutions. These mutations are known to be or are predicted to be resistance mutations to carfentrazone-ethyl, fomesafen, or lactofen.
  • the PPO genes of weeds include the PPO1 gene and the PPO2 gene, and the mutation may be present in either the PPO1 gene or the PPO2 gene, or may be present in both.
  • the mutation is preferably present in the PPO2 gene.
  • Arg128Met means that there is a mutation in the 128th amino acid.
  • the mutation corresponds to the 98th amino acid (Weed Science 60, 335-344), and is known to be represented as Arg98Leu, but this Arg98 is the same as Arg128 in this specification.
  • Arg128Met and Arg128Gly are known in Amaranthus retroflexus (Pest Management Science 73, 1559-1563), Arg128Gly is known in the PPO2 of waterhemp (Pest Management Science, 2019; 75: 3235-3244), Arg128Ile and Arg128Lys are known in the PPO2 of waterhemp (Pest Management Science, 2019; 75: 3235-3244), Arg128His is known as Arg132His in the PPO2 of Bouquet (WSSA annual meeting, 2018), and Gly114Glu, Ser149Ile, Val361Ala, and Gly399Ala are known in the PPO2 of Amaranthus retroflexus (Frontiers in Plant Science 10, Article 568 and Plants 2023, 12(9), 1886), and Ala210Thr is known as Ala212Thr in PPO1 of goosegrass (Pest Management Science, doi: 10.1002/ps.570
  • PPO inhibitor-resistant weeds having these site mutations of action can be effectively controlled, but the PPO inhibitor-resistant weeds to be controlled are not limited to these. That is, not only ambrosia having the Arg128Leu, Arg128Met, Arg128Gly, Arg128His, Arg128Ala, Arg128Cys, Arg128Glu, Arg128Ile, Arg128Lys, Arg128Asn, Arg128Gln, Arg128Ser, Arg128Thr, Arg128Val, Arg128Tyr, Gly210 deficiency, Ala210 deficiency, Gly210Thr, Ala210Thr, Gly211 deficiency, Gly114Glu, Ser149Ile or Gly399Ala mutation in PPO1 or PPO2, but also, for example, waterhemp having the same mutation, ragweed having the same mutation, and dayflower having the same mutation, etc.
  • weeds with reduced susceptibility due to non-acting site mutations include waterhemp and ambrosia, which have become resistant to PPO inhibitors due to the involvement of CYP or GST, and waterhemp, which has become resistant to carfentrazone-ethyl (PLOS ONE, doi: 10.1371/journal.pone.0215431). These can be effectively controlled by the present invention.
  • Auxinic herbicide resistance An example of an active point mutation is a mutation that causes Gly-Asn in the degron region of the AUX/IAA gene. Kochia japonica, Amaranthus retroflexus, and waterhemp that have this mutation can be effectively controlled by the present invention.
  • non-active point mutations examples include dicamba-resistant Amaranthus retroflexus and 2,4-D-resistant waterhemp, which are suggested to be involved in CYP, and these can be effectively controlled by the present invention.
  • HPPD inhibitor resistance Examples of weeds with reduced susceptibility due to non-acting site mutations include waterhemp and amplexicaule, which have become resistant to HPPD inhibitors due to the involvement of CYP or GST, and these can be effectively controlled by the present invention.
  • amplexicaule which is known to overexpress CYP72A219, CYP81B, or CYP81E8.
  • Photosystem II inhibitor resistance Examples of weeds with reduced susceptibility due to site mutation include weeds with one or more of the following amino acid substitutions in the psbA gene: Val219Ile, Ser264Gly, Ser264Ala, and Phe274Val.
  • the present invention effectively controls Amaranthus retroflexus and waterhemp, which have these site mutations and are resistant to photosystem II inhibitors.
  • Examples of weeds with reduced susceptibility due to non-site mutation include Amaranthus retroflexus and waterhemp, which are resistant to photosystem II inhibitors due to the involvement of CYP, GST, or AAA, and are effectively controlled by the present invention.
  • One example of such weeds is Boumugi, which has overexpressed CYP71R4.
  • Glutamate synthetase inhibitor resistance An example of reduced susceptibility of weeds due to an active site mutation is a weed with a mutation that causes an amino acid substitution of Asp171Asn in the glutamine synthetase gene.
  • glutamine synthetase inhibitor-resistant weeds such as Amaranthus retroflexus and waterhemp having this mutation can be effectively controlled.
  • An example of reduced susceptibility of weeds due to a non-active site mutation is Amaranthus retroflexus and waterhemp that have become resistant to glufosinate due to the involvement of CYP or GST, and these can be effectively controlled by the present invention.
  • weeds are Amaranthus retroflexus, which has overexpressed CYP72A219, CYP81B, or CYP81E8.
  • Resistant weeds that are "stacked" with resistance to two or more of the above groups (arbitrarily selected group 2, arbitrarily selected group 3, arbitrarily selected group 4, arbitrarily selected group 5, arbitrarily selected group 6, group 7, group 8) are also effectively controlled.
  • An example of a stacked resistant weed is waterhemp, which is resistant to all of photosystem II inhibitors, HPPD inhibitors, 2,4-D, PPO inhibitors, ALS inhibitors, and glyphosate, and is also effectively controlled.
  • the stack may be a combination of active point mutations, a combination of non-active point mutations, or a combination of active and non-active point mutations.
  • herbicides that may be included in the composition of the present invention, or that may be used in combination with compound X and 2,4-D trolamine salt in the method of the present invention, include the following herbicides. These can also be mixed into the composition of the present invention containing only compound X and 2,4-D trolamine salt as active ingredients.
  • Glyphosate and its salts isopropylammonium salt, ammonium salt, potassium salt, guanidine salt, dimethylamine salt, monoethanolamine salt, choline salt, BAPMA (N,N-bis-(aminopropyl)methylamine) salt), 2,4-D and its salts or esters (ammonium salt, butotyl ester, 2-butoxypropyl ester, butyl ester, diethylammonium salt, dimethylammonium salt, diolamine salt, dodecylammonium salt, ethyl ester, 2-ethylhexyl ester, heptylammonium salt, isobutyl ester, isoctyl ester, isopropyl ester, isopropylammonium salt, lithium salt, meptyl ester, methyl ester, octyl ester, pentyl ester, propyl ester, sodium salt, tefury
  • preferred herbicides that can be used in combination with compound X and 2,4-D-trolamine salt include potassium glyphosate, guanidine glyphosate, dimethylamine glyphosate, monoethanolamine glyphosate, ammonium glufosinate, isopropylammonium glyphosate, diflufenican, rimisoxafen, icaforin methyl, pyroxasulfone, dicamba diglycolamine salt, dicamba BAPMA salt, dicamba TBA salt, dicamba TBP salt, flumiclorac pentyl, clethodim, methoproxybicyclon, lactofen, S-metolachlor, metribuzin, flufenacet, nicosulfuron, rimsulfuron, acetochlor, mesotrione, isoxaflutole, chlorimuron ethyl, thifensulfuron
  • the ratio of the herbicide that can be used in combination with compound X and 2,4-D trolamine salt to compound X is usually in the range of 0.01 to 1000 parts by weight, and preferably in the range of 0.1 to 300 parts by weight.
  • the active ingredient of the herbicide is a salt (e.g., glyphosate potassium salt, glyphosate monoethanolamine salt)
  • the weight means the acid equivalent unless otherwise specified.
  • compound X and 2,4-D trolamine salt in combination with one or more herbicides are compound X + 2,4-D trolamine salt + glyphosate potassium salt, and compound X + 2,4-D trolamine salt + glyphosate monoethanolamine salt.
  • plant nutritional management can be carried out as in general crop cultivation.
  • the fertilization system can be based on Precision Agriculture or a uniform system as per the conventional practice.
  • nitrogen fixing bacteria and mycorrhizal fungi can be inoculated in combination with seed treatment.
  • the herbicidal effect is evaluated on a scale from 0 to 100, with a score of "0" indicating that there is no or almost no difference in the emergence or growth of the test weeds at the time of the investigation compared to untreated control, and a score of "100" indicating that the test weeds are completely dead or the emergence or growth of the test weeds is completely suppressed. Damage to crops caused by pesticides is assessed as "harmless” if little damage is observed, “small” if slight damage is observed, “medium” if moderate damage is observed, and “large” if severe damage is observed.
  • Example 1 Weeds (big weed, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom flower, barnyard grass, and foxtail millet) are sown in plastic pots filled with soil. On the same day, 70 g/ha of flumioxazin plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and soybeans are sown 7 days later. After a further 14 days, the herbicidal effect on the weeds and the chemical damage to the soybeans are investigated. A synergistic weed control effect is confirmed by the combined use of flumioxazin and 2,4-D trolamine salt.
  • Example 2 Weeds (big weed, water hemp, common ragweed, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. On the same day, 140 g/ha of flumioxazin plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and after 21 days, the effect on weeds and the chemical damage to soybeans are investigated. A synergistic weed control effect is confirmed when flumioxazin and 2,4-D trolamine salt are used in combination.
  • Example 3 Weeds (big weed, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom flower, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. The plants are then cultivated in a greenhouse, and 21 days after sowing, 70 g/ha of flumioxazin plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the foliage at a rate of 200 L/ha. The plants are further cultivated in a greenhouse, and 14 days after treatment, the effect on weeds and the phytotoxicity to soybeans are investigated. A synergistic weed control effect is confirmed when flumioxazin and 2,4-D trolamine salt are used in combination.
  • Example 4 Weeds (big weed, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom flower, barnyard grass, and foxtail grass) are sown in plastic pots filled with soil. On the same day, 12.5 g/ha of trifludimoxazine plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and soybeans are sown after 7 days. The herbicidal effect on the weeds and the phytotoxicity to the soybeans are investigated after a further 14 days. A synergistic weed control effect is confirmed by the combined use of trifludimoxazine and 2,4-D trolamine salt.
  • Example 5 Weeds (big weed, water hemp, common ragweed, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. On the same day, 25 g/ha of trifludimoxazine plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and after 21 days, the effects on weeds and the chemical damage to soybeans are investigated. A synergistic weed control effect is confirmed by the combined use of trifludimoxazine and 2,4-D trolamine salt.
  • Example 6 Weeds (big weed, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom flower, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. The plants are then cultivated in a greenhouse, and 21 days after sowing, 12.5 g/ha of trifludimoxazine plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the stems and leaves at a rate of 200 L/ha. The plants are further cultivated in a greenhouse, and 14 days after treatment, the effect on weeds and the phytotoxicity to soybeans are investigated. A synergistic weed control effect is confirmed by the combined use of trifludimoxazine and 2,4-D trolamine salt.
  • Example 7 Weeds (big weed, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom flower, barnyard grass, and foxtail grass) are sown in plastic pots filled with soil. On the same day, 25 g/ha of saflufenacil plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and soybeans are sown after 7 days. The herbicidal effect on the weeds and the chemical damage to the soybeans are investigated after a further 14 days. A synergistic weed control effect is confirmed by the combined use of saflufenacil and 2,4-D trolamine salt.
  • Example 8 Weeds (big weed, water hemp, common ragweed, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. On the same day, 50 g/ha of saflufenacil plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed onto the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and after 21 days, the effect on weeds and the phytotoxicity to soybeans are investigated. A synergistic weed control effect is confirmed when saflufenacil and 2,4-D trolamine salt are used in combination.
  • Example 10 Weeds (big weed, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom, barnyard grass, and foxtail grass) are sown in plastic pots filled with soil. On the same day, 25 g/ha of thiafenacil plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and soybeans are sown after 7 days. The herbicidal effect on the weeds and the phytotoxicity to the soybeans are investigated after a further 14 days. A synergistic weed control effect is confirmed by the combined use of thiafenacil and 2,4-D trolamine salt.
  • Example 11 Weeds (big weed, water hemp, common ragweed, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. On the same day, 50 g/ha of thiafenacil plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and after 21 days, the effect on weeds and the phytotoxicity to soybeans are investigated. A synergistic weed control effect is confirmed when thiafenacil and 2,4-D trolamine salt are used in combination.
  • Example 12 Weeds (big weed, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom flower, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. The plants are then cultivated in a greenhouse, and 21 days after sowing, 25 g/ha of thiafenacil plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the foliage at a rate of 200 L/ha. The plants are further cultivated in a greenhouse, and 14 days after treatment, the effect on weeds and the phytotoxicity to soybeans are investigated. A synergistic weed control effect is confirmed when thiafenacil and 2,4-D trolamine salt are used in combination.
  • Example 13 Weeds (big weed, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom flower, barnyard grass, and foxtail grass) are sown in plastic pots filled with soil. On the same day, 25 g/ha of flufenoximacil plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and soybeans are sown after 7 days. The herbicidal effect on the weeds and the phytotoxicity to the soybeans are investigated after a further 14 days. A synergistic weed control effect is confirmed by the combined use of flufenoximacil and 2,4-D trolamine salt.
  • Example 14 Weeds (big-leaf amaranth, waterhemp, ragweed, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. On the same day, 50 g/ha of flufenoximacil plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and after 21 days, the effect on weeds and the phytotoxicity to soybeans are investigated. A synergistic weed control effect is confirmed when flufenoximacil and 2,4-D trolamine salt are used in combination.
  • Example 15 Weeds (big weed, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom flower, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. The plants are then cultivated in a greenhouse, and 21 days after sowing, 25 g/ha of flufenoximacil plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the foliage at a rate of 200 L/ha. The plants are further cultivated in a greenhouse, and 14 days after treatment, the effect on weeds and the phytotoxicity to soybeans are investigated. A synergistic weed control effect is confirmed when flufenoximacil and 2,4-D trolamine salt are used in combination.
  • Example 16 Weeds (big weed, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom, barnyard grass, and foxtail millet) are sown in plastic pots filled with soil. On the same day, 25 g/ha of compound 1 plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and soybeans are sown after 7 days. The herbicidal effect on the weeds and the phytotoxicity to the soybeans are investigated after a further 14 days. A synergistic weed control effect is confirmed by the combined use of compound 1 and 2,4-D trolamine salt.
  • Example 17 Weeds (Amaranthus retroflexus, water hemp, common ragweed, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. On the same day, 50 g/ha of compound 1 plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and after 21 days, the effects on weeds and phytotoxicity to soybeans are investigated. A synergistic weed control effect is confirmed by the combined use of compound 1 and 2,4-D trolamine salt.
  • Example 18 Weeds (big-leaf amaranth, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom flower, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. The plants are then cultivated in a greenhouse, and 21 days after sowing, 25 g/ha of compound 1 plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the stems and leaves at a rate of 200 L/ha. The plants are further cultivated in a greenhouse, and 14 days after treatment, the effect on weeds and the phytotoxicity to soybeans are investigated.
  • a synergistic weed control effect is confirmed by the combined use of compound 1 and 2,4-D trolamine salt.
  • Example 19 Weeds (big-leaf amaranth, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom, barnyard grass, and foxtail millet) are sown in plastic pots filled with soil. On the same day, 25 g/ha of compound 2 plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and soybeans are sown after 7 days. The herbicidal effect on the weeds and the phytotoxicity to the soybeans are investigated after a further 14 days. A synergistic weed control effect is confirmed by the combined use of compound 2 and 2,4-D trolamine salt.
  • Example 20 Weeds (Amaranthus retroflexus, water hemp, common ragweed, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. On the same day, 50 g/ha of compound 2 plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the soil surface at a spray volume of 200 L/ha. The plants are then cultivated in a greenhouse, and after 21 days, the effects on weeds and the phytotoxicity to soybeans are investigated. A synergistic weed control effect is confirmed by the combined use of compound 2 and 2,4-D trolamine salt.
  • Example 21 Weeds (big-leaf amaranth, water hemp, common ragweed, giant ragweed, artemisia, lamb's paw, broom, barnyard grass, and foxtail grass) and soybeans are sown in plastic pots filled with soil. They are then cultivated in a greenhouse, and 21 days after sowing, 25 g/ha of compound 2 plus 600, 900, or 1200 g/ha of 2,4-D trolamine salt are sprayed on the foliage at a rate of 200 L/ha. They are further cultivated in a greenhouse, and 14 days after treatment, the effect on weeds and the phytotoxicity to soybeans are investigated. A synergistic weed control effect is confirmed by the combined use of compound 2 and 2,4-D trolamine salt.
  • Example 22 to 42 In each of Examples 1 to 21, the spray solution containing Compound X and 2,4-D trolamine salt was similarly treated by adding RoundupWeatherMax (potassium glyphosate 660 g/L, manufactured by Monsanto) to the spray solution so that the application rate was 2.338 L/ha (32 fluid ounces/acre).
  • RoundupWeatherMax potassium glyphosate 660 g/L, manufactured by Monsanto
  • Examples 43 to 63 In each of Examples 22 to 42, the spray solution containing Compound X and 2,4-D trolamine salt was similarly treated by adding XtendiMax (dicamba diglycolamine salt, 350 g/L as dicamba acid, manufactured by Monsanto) at an application rate of 1607 ml/ha (22 fluid ounces/acre).
  • XtendiMax dicamba diglycolamine salt, 350 g/L as dicamba acid, manufactured by Monsanto
  • Examples 64 to 84 In each of Examples 1 to 21, the spray solution containing Compound X and 2,4-D trolamine salt was similarly treated with RoundupExtend (glyphosate monoethanolamine 240 g/L + dicamba diglycolamine 120 g/L, manufactured by Monsanto) at a rate of 4.677 L/ha (64 fluid ounces/acre).
  • RoundupExtend glyphosate monoethanolamine 240 g/L + dicamba diglycolamine 120 g/L, manufactured by Monsanto
  • Examples 85 to 168 Each of Examples 1-84 is run similarly substituting corn or cotton for soybeans.
  • Example 169 NipsIt (clothianidin 600 g/L, Valent) was applied to soybean (Genuity Roundup Ready 2 Yield soybean) seeds at a NipsIt application rate of 206 mL/kg seed (1.28 fluid ounces/100 pounds seed).
  • Valor SX flumioxazin 510 g/kg, Valent
  • formulation Y a formulation containing 2,4-D trolamine salt
  • RoundupWeatherMax potassium glyphosate 660 g/L, manufactured by Monsanto
  • Example 170 NipsIt (clothianidin 600 g/L, Valent) is applied to soybean (Genuity Roundup Ready 2 Yield soybean) seeds at a NipsIt application rate of 206 mL/kg seed (1.28 fluid ounces/100 pounds seed).
  • Vulcarus (trifludimoxazine 415.3 g/kg, BASF) and formulation Y are mixed with water to obtain a spray solution, which is applied to the soybean field before sowing at a rate of 12.5 or 25 g/ha for trifludimoxazine and 600, 900 or 1200 g/ha for 2,4-D trolamine salt.
  • RoundupWeatherMax potassium glyphosate 660 g/L, manufactured by Monsanto
  • Example 171 NipsIt (clothianidin 600 g/L, Valent) is applied to soybean (Genuity Roundup Ready 2 Yield soybean) seeds at a NipsIt application rate of 206 mL/kg seed (1.28 fluid ounces/100 pounds seed). Sharpen (saflufenacil 297.4 g/kg, BASF) and formulation Y are mixed with water to obtain a spray solution, which is sprayed on the soybean field before sowing at a rate of 25 or 50 g/ha for saflufenacil and 600, 900 or 1200 g/ha for 2,4-D trolamine salt.
  • Sharpen saflufenacil 297.4 g/kg, BASF
  • formulation Y are mixed with water to obtain a spray solution, which is sprayed on the soybean field before sowing at a rate of 25 or 50 g/ha for saflufenacil and 600, 900 or 1200 g/ha for 2,4-D trolamine
  • RoundupWeatherMax potassium glyphosate 660 g/L, manufactured by Monsanto
  • Example 172 NipsIt (clothianidin 600 g/L, Valent) is applied to soybean (Genuity Roundup Ready 2 Yield soybean) seeds at a NipsIt application rate of 206 mL/kg seed (1.28 fluid ounces/100 lbs seed).
  • Gamma thiafenacil 700 g/kg, Helm
  • formulation Y are mixed with water to obtain a spray solution, which is sprayed on the soybean field before sowing to obtain a rate of 25 or 50 g/ha of thiafenacil and a rate of 600, 900 or 1200 g/ha of 2,4-D trolamine salt.
  • RoundupWeatherMax potassium glyphosate 660 g/L, manufactured by Monsanto
  • Example 173 Soybean seeds are treated with NipsIt as in Example 169. Flumioxazin is applied at 70 or 140 g/ha, 2,4-D trolamine salt at 600, 900 or 1200 g/ha, and RoundupWeatherMax (potassium glyphosate 660 g/L, Monsanto) at 2.338 L/ha (32 fluid ounces/acre) in a field prior to sowing of the soybeans. Seven days later, the soybeans are sown in the field. When soybeans reach the 3-leaf stage, RoundupWeatherMax (potassium glyphosate 660 g/L, Monsanto) is applied to the field at a rate of 2.338 L/ha (32 fluid ounces/acre).
  • RoundupWeatherMax potassium glyphosate 660 g/L, Monsanto
  • Example 174 Soybean seeds are treated with NipsIt as in Example 169 and sown in a field.
  • Valor SX and Formulation Y are applied to the field at a rate of 70 or 140 g/ha of flumioxazin and 600, 900 or 1200 g/ha of 2,4-D trolamine salt.
  • RoundupWeatherMax 660 g/L potassium glyphosate, Monsanto
  • Valor SX, Formulation Y and RoundupWeatherMax are applied to the field at a rate of 70 or 140 g/ha of flumioxazin, 600, 900 or 1200 g/ha of 2,4-D trolamine salt, and 2.338 L/ha (32 fluid ounces/acre) of RoundupWeatherMax (660 g/L potassium glyphosate, manufactured by Monsanto).
  • RoundupWeatherMax potassium glyphosate 660 g/L, Monsanto
  • 2.338 L/ha 32 fluid ounces/acre
  • Example 176 Soybean seeds are treated with NipsIt as in Example 170.
  • Trifludimoxazine is applied at 12.5 or 25 g/ha
  • 2,4-D trolamine salt is applied at 600, 900 or 1200 g/ha
  • RoundupWeatherMax potassium glyphosate 660 g/L, Monsanto
  • RoundupWeatherMax is applied at 2.338 L/ha (32 fluid ounces/acre) to the field prior to sowing the soybeans, and the field is then sowed 7 days later with Vulcarus, Formulation Y, and RoundupWeatherMax.
  • RoundupWeatherMax is applied to the field at a rate of 2.338 L/ha (32 fluid ounces/acre).
  • Example 177 Soybean seeds are treated with NipsIt and sown in a field as in Example 170.
  • Vulcarus and formulation Y are applied to the field at a rate of 12.5 or 25 g/ha of trifludimoxazine and 600, 900 or 1200 g/ha of 2,4-D trolamine salt.
  • RoundupWeatherMax 660 g/L potassium glyphosate, Monsanto
  • Example 178 Soybean seeds are treated with NipsIt and sown in a field as in Example 170.
  • the day after sowing, Vulcarus, Formulation Y, and RoundupWeatherMax are applied to the field at rates of 12.5 or 25 g/ha trifludimoxazine, 600, 900, or 1200 g/ha 2,4-D trolamine salt, and 2.338 L/ha (32 fluid ounces/acre) RoundupWeatherMax (660 g/L potassium glyphosate, Monsanto).
  • RoundupWeatherMax potassium glyphosate 660 g/L, Monsanto
  • Example 179 Soybean seeds are treated with NipsIt as in Example 171. Saflufenacil is applied at 25 or 50 g/ha, 2,4-D trolamine salt is applied at 600, 900 or 1200 g/ha, and RoundupWeatherMax (potassium glyphosate 660 g/L, Monsanto) is applied at 2.338 L/ha (32 fluid ounces/acre) to the field before sowing the soybeans, and the field is then sown with the soybeans 7 days later. When soybeans reach the 3-leaf stage, RoundupWeatherMax (potassium glyphosate 660 g/L, Monsanto) is applied to the field at a rate of 2.338 L/ha (32 fluid ounces/acre).
  • RoundupWeatherMax potassium glyphosate 660 g/L, Monsanto
  • Example 180 Soybean seeds are treated with NipsIt and sown in a field in the same manner as in Example 171.
  • the day after sowing, Sharpen and formulation Y are applied to the field so that the application rate of saflufenacil is 25 or 50 g/ha and the application rate of 2,4-D trolamine salt is 600, 900 or 1200 g/ha.
  • RoundupWeatherMax 660 g/L potassium glyphosate, manufactured by Monsanto
  • the application rate is 2.338 L/ha (32 fluid ounces/acre).
  • Example 181 Soybean seeds are treated with NipsIt and sown in a field as in Example 171.
  • the day after sowing, Sharpen, Formulation Y, and RoundupWeatherMax are applied to the field at rates of 25 or 50 g/ha of saflufenacil, 600, 900, or 1200 g/ha of 2,4-D trolamine salt, and 2.338 L/ha (32 fluid ounces/acre) of RoundupWeatherMax (660 g/L potassium glyphosate, Monsanto).
  • RoundupWeatherMax potassium glyphosate 660 g/L, Monsanto
  • Example 182 NipsIt is applied to soybean seeds as in Example 172.
  • Thiafenacil is applied at 25 or 50 g/ha
  • 2,4-D trolamine salt is applied at 600, 900 or 1200 g/ha
  • RoundupWeatherMax potassium glyphosate 660 g/L, Monsanto
  • RoundupWeatherMax potassium glyphosate 660 g/L, Monsanto
  • RoundupWeatherMax potassium glyphosate 660 g/L, Monsanto
  • 2.338 L/ha 32 fluid ounces/acre
  • Example 183 Soybean seeds are treated with NipsIt and sown in a field as in Example 172.
  • Gamma and formulation Y are applied to the field at a rate of 25 or 50 g/ha of thiafenacil and 600, 900 or 1200 g/ha of 2,4-D trolamine salt.
  • RoundupWeatherMax 660 g/L potassium glyphosate, Monsanto
  • Example 184 Soybean seeds are treated with NipsIt and sown in a field as in Example 172.
  • the day after sowing, Gamma, Formulation Y, and RoundupWeatherMax are applied to the field at rates of 25 or 50 g/ha of thiafenacil, 600, 900, or 1200 g/ha of 2,4-D trolamine salt, and 2.338 L/ha (32 fluid ounces/acre) of RoundupWeatherMax (660 g/L potassium glyphosate, Monsanto).
  • RoundupWeatherMax potassium glyphosate 660 g/L, Monsanto
  • Examples 185 to 200 In each of Examples 169 to 184, when RoundupWeatherMax is applied the day after sowing or at the soybean 3-leaf stage, XtendiMax (dicamba diglycolamine salt, 350 g/L as dicamba acid, manufactured by Monsanto) is applied in addition to RoundupWeatherMax at a rate of 1607 ml/ha (22 fluid ounces/acre).
  • XtendiMax dicamba diglycolamine salt, 350 g/L as dicamba acid, manufactured by Monsanto
  • Examples 201 to 216 In each of Examples 169-184, the day after sowing or at the soybean 3-leaf stage, instead of applying RoundupWeatherMax, RoundupExtend (glyphosate monoethanolamine 240 g/L + dicamba diglycolamine 120 g/L, Monsanto) is applied at a rate of 4.677 L/ha (64 fluid ounces/acre) of RoundupExtend.
  • RoundupExtend glyphosate monoethanolamine 240 g/L + dicamba diglycolamine 120 g/L, Monsanto
  • Examples 217 to 264 In each of Examples 169-216, INOVATE (clothianidin 160 g/L + metalaxyl 13 g/L + ipconazole 8 g/L, Valent) was used in place of NipsIt, with the INOVATE treatment rate being 309 mL/100 kg seeds (4.74 fluid ounces/100 pounds seeds).
  • Examples 265 to 312 In each of Examples 169 to 216, NipsIt was replaced with CruiserMAXX Vibrance (thiamethoxam 240 g/L + metalaxyl M 36 g/L + fludioxonil 12 g/L + sedaxane 12 g/L, Syngenta) at a treatment rate of 235 mL/100 kg seeds (3.22 fluid ounces/100 pounds seeds).
  • CruiserMAXX Vibrance thiamethoxam 240 g/L + metalaxyl M 36 g/L + fludioxonil 12 g/L + sedaxane 12 g/L, Syngenta
  • Examples 313 to 360 In each of Examples 169-216, instead of treating soybean seeds with NipsIt, they are treated with the Acceleron system (DX-612 (Fluxapyroxad 326 g/L, Monsanto) 31 ml/100 kg seed + DX-309 (Metalaxyl 313 g/L, Monsanto) 242 ml/100 kg seed (1.5 fl oz/100 lb seed) + DX-109 (Pyraclostrobin 200 g/L, Monsanto) 242 ml/100 kg seed (1.5 fl oz/100 lb seed) + IX-104 (Imidacloprid 600 g/L, Monsanto) 515 ml/100 kg seed (3.2 fl oz/100 lb seed)).
  • DX-612 Fluluxapyroxad 326 g/L, Monsanto
  • DX-309 Metalaxyl 313 g/L
  • Examples 361 to 552 In each of Examples 169-360, corn seeds or cotton seeds are substituted for the soybean seeds.
  • Examples 553 to 936 In each of Examples 169 to 552, the same procedure is carried out except that the cultivated crop is replaced with a crop having the Roundup Ready 2 Xtend trait.
  • Examples 937 to 1320 In each of Examples 169 to 552, the same procedure was carried out except that the cultivated crop was replaced with a crop having the Roundup Ready 2 Xtend trait and the PPO inhibitor resistance trait.
  • Examples 1321 to 1704 In each of Examples 169 to 552, the same procedure was carried out except that the cultivated crop was replaced with a crop having the Roundup Ready 2 Xtend trait, the PPO inhibitor resistance trait, and the HPPD inhibitor resistance trait.
  • the present invention allows for highly effective weed control.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne une composition herbicide et un procédé de lutte contre les mauvaises herbes qui ont un excellent effet de lutte contre les mauvaises herbes. L'invention concerne une composition herbicide comprenant un sel de 2,4-D-trolamine et au moins un composé choisi parmi le groupe de composé X. Le groupe de composé X est constitué de flumioxazine, de trifludimoxazine, de saflufénacil, de tiafénacil, de flufénoximacil, de composés représentés par la formule (I), et de composés représentés par la formule (II).
PCT/JP2024/011918 2023-03-31 2024-03-26 Composition herbicide et procédé de lutte contre les mauvaises herbes Ceased WO2024204178A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2023057528 2023-03-31
JP2023-057528 2023-03-31

Publications (1)

Publication Number Publication Date
WO2024204178A1 true WO2024204178A1 (fr) 2024-10-03

Family

ID=92906565

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2024/011918 Ceased WO2024204178A1 (fr) 2023-03-31 2024-03-26 Composition herbicide et procédé de lutte contre les mauvaises herbes

Country Status (2)

Country Link
AR (1) AR132214A1 (fr)
WO (1) WO2024204178A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016536289A (ja) * 2013-10-11 2016-11-24 ダウ アグロサイエンシィズ エルエルシー 水性除草剤濃縮物
JP2019516755A (ja) * 2016-05-24 2019-06-20 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ ウラシルピリジン除草剤
US20220322665A1 (en) * 2019-08-09 2022-10-13 Sumitomo Chemical Company, Limited Method for controlling herbicide-resistant weed
US20220322666A1 (en) * 2019-08-29 2022-10-13 Sumitomo Chemical Company, Limited Herbicidal agent composition and weed control method
JP2023500353A (ja) * 2019-11-07 2023-01-05 チンタオ、キングアグルート、ケミカル、コンパウンド、カンパニー、リミテッド 置換イソキサゾリン含有芳香族化合物、その調製方法、その除草剤組成物及びその使用
WO2024013015A1 (fr) * 2022-07-11 2024-01-18 Bayer Aktiengesellschaft Compositions herbicides

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016536289A (ja) * 2013-10-11 2016-11-24 ダウ アグロサイエンシィズ エルエルシー 水性除草剤濃縮物
JP2019516755A (ja) * 2016-05-24 2019-06-20 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ ウラシルピリジン除草剤
US20220322665A1 (en) * 2019-08-09 2022-10-13 Sumitomo Chemical Company, Limited Method for controlling herbicide-resistant weed
US20220322666A1 (en) * 2019-08-29 2022-10-13 Sumitomo Chemical Company, Limited Herbicidal agent composition and weed control method
JP2023500353A (ja) * 2019-11-07 2023-01-05 チンタオ、キングアグルート、ケミカル、コンパウンド、カンパニー、リミテッド 置換イソキサゾリン含有芳香族化合物、その調製方法、その除草剤組成物及びその使用
WO2024013015A1 (fr) * 2022-07-11 2024-01-18 Bayer Aktiengesellschaft Compositions herbicides

Also Published As

Publication number Publication date
AR132214A1 (es) 2025-06-04

Similar Documents

Publication Publication Date Title
US12564192B2 (en) Herbicidal agent composition and weed control method
US12426598B2 (en) Method for controlling herbicide-resistant weed
US12557814B2 (en) Herbicidal composition and method for controlling weeds
WO2022138632A1 (fr) Composition herbicide et procédé de lutte contre les mauvaises herbes
WO2020009249A2 (fr) Procédé de lutte contre les mauvaises herbes
WO2020137868A1 (fr) Procédé de lutte contre les mauvaises herbes
WO2023228935A1 (fr) Composition herbicide et procédé de lutte contre les mauvaises herbes
WO2022138633A1 (fr) Procédé de lutte contre des mauvaises herbes résistantes à des herbicides
JP2023174423A (ja) 除草剤組成物及び雑草防除方法
WO2024204178A1 (fr) Composition herbicide et procédé de lutte contre les mauvaises herbes
WO2025053005A1 (fr) Composition herbicide et procédé de lutte contre les mauvaises herbes
AU2020248763A1 (en) Method for controlling weeds
CA3286371A1 (fr) Composition herbicide et procédé de lutte contre les mauvaises herbes
WO2026004676A1 (fr) Procédé de lutte contre les mauvaises herbes
WO2023162542A1 (fr) Procédé pour terminer la croissance d'une culture de couverture
WO2020137867A1 (fr) Procédé de lutte contre les mauvaises herbes
WO2020196285A1 (fr) Procédé de lutte contre les mauvaises herbes
BR112021008676B1 (pt) Composição herbicida e método de controle de ervas daninhas
BR112021017579B1 (pt) Método para o controle de ervas daninhas, formulação e uso
BR122025017531A2 (pt) Método de controle de ervas daninhas em uma área de cultivo de soja
BR122025017533A2 (pt) Composição herbicida compreendendo trifludimoxazina, saflufenacil e piroxassulfona
WO2020137866A1 (fr) Procédé de lutte contre les mauvaises herbes
BR122025027993A2 (pt) Método para o controle de ervas daninhas
BR122025017534A2 (pt) Composição herbicida compreendendo trifludimoxazina e pelo menos um herbicida

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24780290

Country of ref document: EP

Kind code of ref document: A1

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112025020054

Country of ref document: BR

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 24780290

Country of ref document: EP

Kind code of ref document: A1