WO2024254462A3 - Methods and kits configured for high-throughput interrogation of nucleic acid-containing samples with high dynamic range - Google Patents

Methods and kits configured for high-throughput interrogation of nucleic acid-containing samples with high dynamic range Download PDF

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Publication number
WO2024254462A3
WO2024254462A3 PCT/US2024/033027 US2024033027W WO2024254462A3 WO 2024254462 A3 WO2024254462 A3 WO 2024254462A3 US 2024033027 W US2024033027 W US 2024033027W WO 2024254462 A3 WO2024254462 A3 WO 2024254462A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
methods
dynamic range
containing samples
kits
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2024/033027
Other languages
French (fr)
Other versions
WO2024254462A2 (en
Inventor
Mingjie DAI
Yan Yan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rice University
Original Assignee
Rice University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rice University filed Critical Rice University
Priority to CN202480040982.9A priority Critical patent/CN121464225A/en
Priority to EP24820136.0A priority patent/EP4724600A2/en
Priority to JP2025571180A priority patent/JP2026519816A/en
Publication of WO2024254462A2 publication Critical patent/WO2024254462A2/en
Publication of WO2024254462A3 publication Critical patent/WO2024254462A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This application describes compositions, methods, and kits for simultaneous, multiplexed detection and quantification of nucleic acid molecules. In particular, compression PCR is disclosed for compressing the dynamic range of highly complex biological samples, and improve the accuracy and efficiency of high-throughput sequencing analysis.
PCT/US2024/033027 2023-06-08 2024-06-07 Methods and kits configured for high-throughput interrogation of nucleic acid-containing samples with high dynamic range Ceased WO2024254462A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN202480040982.9A CN121464225A (en) 2023-06-08 2024-06-07 Methods and kits configured for high throughput interrogation of nucleic acid-containing samples with high dynamic range
EP24820136.0A EP4724600A2 (en) 2023-06-08 2024-06-07 Methods and kits configured for high-throughput interrogation of nucleic acid-containing samples with high dynamic range
JP2025571180A JP2026519816A (en) 2023-06-08 2024-06-07 Methods and kits designed for high-throughput testing of nucleic acid-containing samples with a high dynamic range.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363506995P 2023-06-08 2023-06-08
US63/506,995 2023-06-08

Publications (2)

Publication Number Publication Date
WO2024254462A2 WO2024254462A2 (en) 2024-12-12
WO2024254462A3 true WO2024254462A3 (en) 2025-05-08

Family

ID=93794616

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/033027 Ceased WO2024254462A2 (en) 2023-06-08 2024-06-07 Methods and kits configured for high-throughput interrogation of nucleic acid-containing samples with high dynamic range

Country Status (4)

Country Link
EP (1) EP4724600A2 (en)
JP (1) JP2026519816A (en)
CN (1) CN121464225A (en)
WO (1) WO2024254462A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2769007B1 (en) * 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
US20190153508A1 (en) * 2017-11-22 2019-05-23 Safeguard Biosystems Holdings Ltd. Asymmetric pcr methods, primers and kits

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2769007B1 (en) * 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
US20190153508A1 (en) * 2017-11-22 2019-05-23 Safeguard Biosystems Holdings Ltd. Asymmetric pcr methods, primers and kits

Also Published As

Publication number Publication date
CN121464225A (en) 2026-02-03
EP4724600A2 (en) 2026-04-15
WO2024254462A2 (en) 2024-12-12
JP2026519816A (en) 2026-06-18

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