WO2024255812A1 - Chip positioning apparatus, chip outer frame apparatus for gene sequencer, and gene sequencer - Google Patents

Abstract

Provided are a chip positioning apparatus, a chip outer frame apparatus for a gene sequencer, and a gene sequencer. The chip positioning apparatus is used for positioning a chip, and comprises: a base used for carrying said chip; a first limiting structure fixedly connected to the base and used for abutting against the chip so as to limit the movement of the chip in a first direction and a second direction, the first direction and the second direction being perpendicular to one another; a second limiting structure movably connected to the base and used for abutting against the chip so as to limit the movement of the chip in the direction opposite the first direction and the direction opposite the second direction; and an execution structure connected to the base and abutting against and holding the second limiting structure, used for pushing the second limiting structure to move in the first direction and the second direction such that the second limiting structure abuts against the chip. By using the execution structure, the position of the chip in the first direction on the horizontal plane and the position of the chip in the second direction on the horizontal plane can be determined simultaneously, thus effectively improving chip positioning efficiency.

Description

Chip positioning device, chip outer frame device for gene sequencer and gene sequencer

This application claims the priority of the Chinese patent filed with the Chinese Patent Office on July 28, 2023, with application number 202322038680.3 and application name “Chip positioning device and chip processing device”; the Chinese patent filed with the Chinese Patent Office on November 22, 2023, with application number 202323165477.9 and application name “A chip holder for a gene sequencer”; and the Chinese patent filed with the Chinese Patent Office on June 13, 2023, with application number 202310699027.3 and application name “A chip frame device for a gene sequencer”, all of which are incorporated by reference in this application.

Technical Field

The present application relates to the field of chip processing gene sequencing, and specifically, to a chip positioning device, a chip frame device for a gene sequencer, and a gene sequencer.

Background Art

When a biochip is in use, it is necessary to fix the chip at a preset position. The accuracy of its position is directly related to the accuracy of the experimental results. In the related art, the process of fixing the position of the biochip is relatively cumbersome and needs to be fixed separately from different directions, which is not easy to operate.

Summary of the invention

The purpose of the present application is to provide a chip positioning device, a chip frame device for a gene sequencer, and a gene sequencer, which can quickly position the chip.

The embodiment of the present application is implemented as follows:

In a first aspect, a chip positioning device is provided for positioning a chip in a gene sequencer, the device comprising:

A base, used for carrying the chip;

A first limiting structure, fixedly connected to the base, used to abut against the chip to limit the chip from moving along a first direction and a second direction; wherein the first direction and the second direction are perpendicular to each other;

a second limiting structure, movably connected to the base, and used to abut against the chip to limit the movement of the chip in a direction opposite to the first direction and in a direction opposite to the second direction;

The execution structure is connected to the base and abuts against the second limiting structure, and is used to push the second limiting structure to move along the first direction and the second direction to abut against the chip.

In a specific possible implementation manner, the second limiting structure forms an abutment surface, and the abutment surface is inclined from a direction opposite to the first direction toward a direction opposite to the second direction;

The execution structure includes an abutment member and an elastic member; the elastic member is connected to the base and the abutment member to push the abutment member to move; the abutment member abuts against the abutment surface to push the second limiting structure to move along the first direction and the second direction under the action of the elastic member.

In a specific possible implementation manner, the abutment member is rotatably connected to the base, and a rotation axis of the abutment member is parallel to the second direction.

In a specific possible implementation manner, one end of the elastic member is fixedly connected to the base, and the other end of the elastic member is fixedly connected to one end of the abutment member, and the elastic member is used to push the abutment member to rotate toward a direction close to the second limiting structure.

In a specific possible implementation scheme, a pressing portion is formed on the abutment member, and the pressing portion is arranged on a side of the abutment member away from the elastic member. The pressing portion is used to receive external force and rotate in a direction away from the second limiting structure under the action of the external force.

In a specific implementation scheme, the first limiting structure includes:

A first limiting member, fixedly connected to the base, and used to abut against the chip to limit the movement of the chip along the first direction;

The second limiting member is fixedly connected to the base and is used to abut against the chip to limit the chip from moving along the The second direction of movement.

In a specific possible implementation scheme, the second limiting structure is annular, and a cavity for accommodating the chip is formed inside the second limiting structure; the second limiting structure has a first inner wall and a second inner wall, the first inner wall is used to abut against the chip to limit the movement of the chip in the opposite direction of the first direction; the second inner wall is used to abut against the chip to limit the movement of the chip in the opposite direction of the second direction.

In a specific possible implementation manner, the second limiting structure forms an opening, the opening is connected to the cavity, and the opening is used to accommodate the first limiting member and the second limiting member.

In a specific embodiment, it also includes:

The cover body is rotatably connected to the base and is used for pressing the chip when the cover body is close to the base.

In a specific possible implementation manner, at least two groups of pressing claw assemblies are symmetrically installed on the lower surface of the cover body, and the at least two groups of pressing claw assemblies abut against the chip when the cover body is close to the base, so as to press and fix the chip.

In a specific possible implementation manner, the pressure claw assembly includes a pressure claw frame, a rotating shaft and a pressure claw, the pressure claw frame is rotatably connected to the rotating shaft, the pressure claw abuts between the pressure claw frame and the rotating shaft, and the pressure claw is used to press the chip.

In a specific possible implementation scheme, the pressure claw assembly also includes a spring, one end of the pressure claw is used to press the chip, and the other end of the pressure claw is fixedly connected to the pressure claw frame through the spring.

In a specific possible implementation manner, the pressure jaw assembly further includes a supporting member, the pressure jaw is disposed between the supporting member and the rotating shaft, and a middle portion of the pressure jaw abuts against the supporting member.

In a specific embodiment, the contact point between the pressing claw and the supporting member has an arc-shaped convex surface, and the pressing claw can rotate along the arc-shaped convex surface to adapt to the angle of the chip.

In a specific possible implementation mode, a limiting groove is provided on the top of the pressing claw, and the rotating shaft passes through the limiting groove and is rotatably connected to the pressing claw frame.

In a specific possible implementation manner, the contact surface between the pressure claw and the chip is a flexible structure, and the flexible structure is one of a rubber pad, a sponge pad and a polyurethane foam.

In a specific implementation scheme, the chip is provided with a plurality of flow channels arranged along its length direction, and fluid ports are respectively provided on both sides of the flow channels along the thickness direction of the chip, and the fluid ports are connected to the flow channel ports in the base.

In a second aspect, a chip frame device for a gene sequencer is provided, the device comprising a chip frame, a chip and a sealing gasket, the chip frame is provided with a chip slot, the chip is arranged in the chip slot, and the chip slot is provided with a first fixing mechanism for fixing the chip;

The sealing gasket is detachably mounted on the bottom of the chip frame;

The chip is provided with a plurality of transverse and mutually parallel fluid grooves inside, the lower end surface of the chip is provided with a fluid port connected with both ends of the fluid grooves, the sealing gasket is provided with a fluid circular hole, and the fluid port and the fluid circular hole are connected in sequence.

In a specific possible implementation manner, the sealing gasket is provided with a plug-in groove for engaging with the plug-in boss of the gene sequencer.

In a specific implementation scheme, the first fixing mechanism includes an upper limit plate and a lower limit plate, and there are at least one pair of the upper limit plate and the lower limit plate respectively. The at least one pair of upper and lower limit plates are respectively fixedly mounted on the inner walls on opposite sides of the chip frame, and the chip is accommodated in a groove formed between the upper limit plate and the lower limit plate.

In a specific implementation scheme, the first fixing mechanism also includes a limiting boss, which is fixedly mounted on the inner side wall of the chip frame, and at least one limiting boss is arranged on each inner side wall of the chip frame, and the limiting boss has a contact surface that conflicts with the side wall of the chip.

In a specific implementation manner, the contact surface is a plane, a curved surface or a spherical surface.

In a specific embodiment, the limiting boss located on the front side is an elastic boss, which is fixedly mounted on the inner wall of the chip outer frame through an elastic sheet and has a pressing force toward the inner side of the chip slot. Elasticity.

In a specific possible implementation manner, the limiting boss located on the left side has an inclined surface that is inclined from top to bottom toward the inside, and the lower end of the inclined surface is transitionally connected to the contact surface on the limiting boss.

In a specific implementation scheme, a hook is fixedly provided at the bottom of the chip frame, a hanging hole for the hook to be inserted is opened on the sealing gasket, and the sealing gasket is connected to the hook at the bottom of the chip frame through the hanging hole.

In a specific possible implementation scheme, a limiting notch is provided on the chip outer frame and corresponds to a positioning reference on the gene sequencer, and the positioning reference is embedded in the limiting notch.

In a specific possible implementation scheme, the outer side wall of the chip frame has a positioning groove for positioning and cooperating with a positioning element on the gene sequencer, and when the positioning element is in a retracted position, the positioning element is separated from the positioning groove.

In a third aspect, a gene sequencer is provided, the gene sequencer comprising a chip platform for carrying a chip;

A first limiting structure, fixedly connected to the chip platform, used to abut against the chip to limit the movement of the chip along a first direction and a second direction; wherein the first direction and the second direction are perpendicular to each other;

A chip outer frame, movably connected to the chip platform, the chip outer frame is provided with a chip slot, the chip is arranged in the chip slot, so as to limit the movement of the chip in a direction opposite to the first direction and a direction opposite to the second direction;

An execution structure, connected to the chip platform and abutting against the chip outer frame, used for pushing the chip outer frame to move along the first direction and the second direction to abut against the chip;

A sealing gasket, detachably mounted on the bottom of the chip frame;

A sealing block corresponding to the position of the sealing pad is arranged on the chip platform;

The chip is provided with a plurality of transverse and mutually parallel fluid grooves inside, the lower end surface of the chip is provided with a fluid port connected with the two ends of the fluid groove, the sealing gasket is provided with a fluid circular hole, the sealing block is provided with a flow channel opening, and the fluid port, the fluid circular hole and the flow channel opening are connected in sequence.

In a specific possible implementation manner, the chip outer frame forms an abutting surface, and the abutting surface is inclined from a direction opposite to the first direction toward a direction opposite to the second direction;

The execution structure includes an abutment member and an elastic member; the elastic member is connected to the chip platform and the abutment member to push the abutment member to move; the abutment member abuts against the abutment surface to push the chip frame to move along the first direction and the second direction under the action of the elastic member.

In a specific possible implementation manner, the abutment member is rotatably connected to the chip platform, and a rotation axis of the abutment member is parallel to the second direction.

In a specific implementation scheme, one end of the elastic member is fixedly connected to the chip platform, and the other end of the elastic member is fixedly connected to one end of the abutment member, and the elastic member is used to push the abutment member to rotate toward the direction close to the chip frame.

In a specific implementation scheme, a pressing portion is formed on the abutment member, and the pressing portion is arranged on a side of the abutment member away from the elastic member. The pressing portion is used to receive external force and rotate in a direction away from the chip frame under the action of the external force.

In a specific implementation scheme, the first limiting structure includes:

A first limiting member, fixedly connected to the chip platform, and used to abut against the chip to limit the movement of the chip along the first direction;

The second limiting member is fixedly connected to the chip platform and is used for abutting against the chip to limit the movement of the chip along the second direction.

In a specific possible implementation scheme, the chip frame is annular, and a cavity for accommodating the chip is formed inside the chip frame; the chip frame has a first inner wall and a second inner wall, the first inner wall is used to abut against the chip to limit the movement of the chip in the opposite direction of the first direction; the second inner wall is used to abut against the chip to limit the movement of the chip in the opposite direction of the second direction.

In a specific embodiment, the chip frame forms an opening, and the opening is connected to the cavity. The opening is used to accommodate the first limiting member and the second limiting member.

In a specific embodiment, it also includes:

The cover body is rotatably connected to the chip platform and is used for pressing the chip when the cover body is close to the chip platform.

In a specific possible implementation manner, at least two groups of pressing claw assemblies are symmetrically installed on the lower surface of the cover body, and the at least two groups of pressing claw assemblies abut against the chip when the cover body is in a closed state, so as to press and fix the chip.

In a specific possible implementation manner, the pressure claw assembly includes a pressure claw frame, a rotating shaft and a pressure claw, the pressure claw frame is rotatably connected to the rotating shaft, the pressure claw abuts between the pressure claw frame and the rotating shaft, and the pressure claw is used to press the chip.

In a specific possible implementation scheme, the pressure claw assembly also includes a spring, one end of the pressure claw is used to press the chip, and the other end of the pressure claw is fixedly connected to the pressure claw frame through the spring.

In a specific possible implementation manner, the pressure jaw assembly further includes a supporting member, the pressure jaw is disposed between the supporting member and the rotating shaft, and a middle portion of the pressure jaw abuts against the supporting member.

In a specific embodiment, the contact point between the pressing claw and the supporting member has an arc-shaped convex surface, and the pressing claw can rotate along the arc-shaped convex surface to adapt to the angle of the chip.

In a specific possible implementation mode, a limiting groove is provided on the top of the pressing claw, and the rotating shaft passes through the limiting groove and is rotatably connected to the pressing claw frame.

In a specific feasible implementation scheme, a first fixing mechanism for fixing the chip is provided on the chip slot; the first fixing mechanism includes an upper limit plate and a lower limit plate, and there are at least one pair of upper limit plates and lower limit plates respectively, and at least one pair of upper and lower limit plates are respectively fixedly installed on the inner walls on opposite sides of the chip outer frame, and the chip is accommodated in the groove formed between the upper limit plate and the lower limit plate.

In a specific implementation scheme, the first fixing mechanism also includes a limiting boss, which is fixedly mounted on the inner side wall of the chip frame, and at least one limiting boss is arranged on each inner side wall of the chip frame, and the limiting boss has a contact surface that conflicts with the side wall of the chip.

In a specific implementation manner, the contact surface is a plane, a curved surface or a spherical surface.

In a specific possible implementation manner, the limiting boss located on the front side is an elastic boss, which is fixedly mounted on the inner wall of the chip outer frame through an elastic sheet and has an elastic force that presses toward the inner side of the chip slot.

In a specific possible implementation manner, the limiting boss located on the left side has an inclined surface that is inclined from top to bottom toward the inside, and the lower end of the inclined surface is transitionally connected to the contact surface on the limiting boss.

The beneficial effects of this application are:

The embodiment of the present application positions the chip at different positions through the cooperation of various mechanisms, which can effectively improve the positioning efficiency of the chip.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only illustrate certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in the field of gene sequencing, other relevant drawings can be obtained based on these drawings without creative work.

FIG1 is a schematic diagram of the structure of a chip positioning device provided in an embodiment of the present application;

FIG2 is a cross-sectional view of a chip positioning device provided in an embodiment of the present application;

FIG3 is a schematic diagram of a chip base structure used in a gene sequencer of the present application;

FIG4 is a schematic diagram showing the disassembled structure of the sequencing chip of FIG3 of the present application;

FIG5 is a schematic diagram showing the structure of the pressure claw assembly in FIG3 of the present application;

FIG6 is a schematic diagram of a side cross-sectional structure of FIG5 of the present application;

FIG. 7 is a schematic diagram of the front view structure of FIG. 5 of the present application.

FIG8 is a schematic diagram of the structure of a chip frame device for a gene sequencer;

FIG9 is a schematic diagram of the back structure of a chip frame device for a gene sequencer;

FIG10 is a top view of a chip frame device for a gene sequencer;

FIG11 is a cross-sectional view of a hook in a chip frame device for a gene sequencer;

FIG12 is a schematic diagram of the installation structure of a chip frame and a chip platform in a chip frame device for a gene sequencer;

FIG13 is a schematic diagram of the structure of a chip platform in a chip frame device for a gene sequencer;

icon:
100, chip split positioning device; 110, base; 120, first limiting structure; 121,
First limiting member; 122, second limiting member; 130, second limiting structure; 131, abutting surface; 132, first inner wall; 133, second inner wall; 140, execution structure; 141, abutting member; 142, elastic member; 150, cover body; 200, chip. 1. Sequencing chip; 2. Sequencing chip platform; 3. Pressure cover; 4. Pressure claw assembly; 41. Pressure claw frame; 42. Rotating shaft; 43. Pressure claw; 44. Spring; 45. Support member; 46. Limiting groove; 5. Chip positioning block; 6. Chip frame; 7. Flow channel; 8. Fluid port; 9. Flow channel port; 11. Sealing structure; Chip outer frame 10, hook 101, elastic boss 102, limiting boss 103, upper limiting plate 104, lower limiting plate 105, positioning groove 106, limiting notch 107; Sequencing chip 20, fluid port 201, fluid groove 202; Sealing gasket 30, fluid circular hole 301, hanging hole 302, plug-in groove 303; Chip platform 40, positioning reference 401, positioning element 402, positioning head 402a; Sealing block 50, plug-in boss 501, flow channel port 502.

DETAILED DESCRIPTION

In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the present application for which protection is sought, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field of gene sequencing without creative work are within the scope of protection of the present application.

It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

In the description of the embodiments of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or are the positions or positional relationships in which the product of the application is usually placed when in use. They are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

In addition, the terms "horizontal", "vertical", "overhanging" and the like do not mean that the components are required to be absolutely horizontal or overhanging, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

In the description of the embodiments of the present application, it should also be noted that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal connection of two elements. For ordinary technicians in the field of gene sequencing, the specific meanings of the above terms in the embodiments of the present application can be understood according to specific circumstances.

Referring to FIG. 1 and FIG. 2 , an embodiment of the present application provides a chip split positioning device 100, the chip split positioning device 100 comprises: a base 110, a first limiting structure 120, a second limiting structure 130 and an execution structure 140.

The base 110 is used to carry the chip 200. Specifically, a plane may be formed on the base 110, and the plane may be parallel to the horizontal plane. When the chip 200 is carried on the base 110, the chip 200 is on the horizontal plane, and the position of the chip 200 can be determined by controlling the position of the chip 200 on the horizontal plane.

The first limiting structure 120 is fixedly connected to the base 110, and the first limiting structure 120 is used to abut against the chip 200. When the first limiting structure 120 abuts against the chip 200, the first limiting structure 120 can limit the movement of the chip 200 along the first direction and the second direction. The first direction and the second direction can both be horizontal directions, and the first direction and the second direction are perpendicular to each other.

Specifically, the first limiting structure 120 may include: a first limiting member 121 and a second limiting member 122, wherein the first limiting member 121 is fixedly connected to the base 110 and is used to abut against the chip 200 to limit the movement of the chip 200 in the first direction. That is, the first limiting member 121 is arranged on the path of the chip 200 moving in the first direction to prevent the chip 200 from continuing to move in the first direction. The second limiting member 122 is fixedly connected to the base 110 and is used to abut against the chip 200 to limit the movement of the chip 200 in the second direction. That is, the second limiting member 122 is arranged on the path of the chip 200 moving in the second direction to prevent the chip 200 from continuing to move in the second direction. Specifically, the first limiting member 121 and the second limiting member 122 can both be set as columnar structures, and the side walls of the columnar structures can abut against the chip 200 to prevent the chip 200 from moving.

The second limiting structure 130 is movably connected to the base 110 and is used to abut against the chip 200. When the second limiting structure 130 abuts against the chip 200, the second limiting structure 130 can limit the chip 200 from moving in the opposite direction of the first direction and the opposite direction of the second direction.

The chip positioning device provided in the embodiment of the present application includes: a base, a first limiting structure, a second limiting structure and an execution structure. The base is used to carry the chip. The base can be used to place the chip on a horizontal plane to determine the position of the chip in the vertical direction; the first limiting structure is fixedly connected to the base, the first limiting structure is used to abut against the chip, and the first limiting structure is used to limit the movement of the chip along the first direction and the second direction. Both the first direction and the second direction can be horizontal directions. In the horizontal direction, the chip only needs to be tightly fitted to the first limiting structure to fix the position of the chip on the horizontal plane; the second limiting structure is movably connected to the base, the second limiting structure is used to abut against the chip, and the second limiting structure is used to limit the movement of the chip in the opposite direction of the first direction and the opposite direction of the second direction. The second limiting structure can be used to abut the first limiting structure to fix the position of the chip; the execution structure is connected to the base and abuts against the second limiting structure. The execution structure is used to push the second limiting structure to move along the first direction and the second direction to abut against the chip; the execution structure can be used to push the second limiting structure to move along the first direction and the second direction, so that the chip is tightly fitted to the first limiting structure and the second limiting structure, thereby determining the position of the chip.

Therefore, the execution structure can be used to simultaneously determine the position of the chip in the first direction and the position of the chip in the second direction on the horizontal plane, which can effectively improve the positioning efficiency of the chip.

Specifically, the second limiting structure 130 can be set to be annular, and the interior of the second limiting structure 130 forms a cavity for accommodating the chip 200. When the chip 200 is placed in the cavity, the two inner walls of the second limiting structure 130, namely the first inner wall 132 and the second inner wall 133, can be closely fitted to the chip 200. The first inner wall 132 is used to abut against the chip 200 to limit the movement of the chip 200 in the opposite direction of the first direction; the second inner wall 133 is used to abut against the chip 200 to limit the movement of the chip 200 in the opposite direction of the second direction. The first inner wall 132 and the second inner wall 133 can be perpendicular to each other. Since the second limiting structure 130 is an annular structure, the relative position relationship between the first inner wall 132 and the second inner wall 133 remains fixed. Therefore, by controlling the second limiting structure 130, the first inner wall 132 and the second inner wall 133 can be controlled simultaneously, so as to facilitate the determination of the position of the chip 200.

The execution structure 140 is connected to the base 110 and abuts against the second limiting structure 130. The execution structure 140 is used to push the second limiting structure 130 to move along the first direction and the second direction so that the second limiting structure 130 abuts against the chip 200. The execution structure 140 can be used to push the second limiting structure 130 to move along the first direction and the second direction so as to abut against the chip 200, and push the chip 200 to abut against the first limiting structure 120, so as to determine the position of the chip 200.

Specifically, the second limit structure 130 forms an abutting surface 131, and the abutting surface 131 is inclined from the opposite direction of the first direction to the opposite direction of the second direction. The structure 130 moves in the first direction and the second direction simultaneously.

The execution structure 140 includes an abutment member 141 and an elastic member 142. The abutment member 141 abuts against the abutment surface 131 to push the second position-limiting structure 130 to move together in the first direction and the second direction. The elastic member 142 is connected to the base 110 and the abutment member 141. The elastic member 142 is used to push the abutment member 141 to move. Under the action of the elastic member 142, the abutment member 141 can be kept in a state of abutting against the second position-limiting member 122 structure, thereby ensuring the stability of the position of the chip 200.

In some embodiments of the present application, the abutment 141 is rotatably connected to the base 110, and the rotation axis of the abutment 141 is parallel to the second direction. Specifically, by using the abutment 141 to be rotatably connected to the base 110, not only can the abutment 141 be moved closer to or farther from the second position-limiting structure 130 by rotation, but the position of the abutment 141 can also be kept stable to prevent the abutment 141 from separating from the base 110.

Specifically, in some embodiments of the present application, one end of the elastic member 142 is fixedly connected to the base 110, and the other end of the elastic member 142 is fixedly connected to one end of the abutting member 141, and the elastic member 142 is used to push the abutting member 141 to rotate in a direction close to the second limiting structure 130. Specifically, in the process of the abutting member 141 rotating toward the second limiting structure 130, the end of the abutting member 141 abuts against the abutting surface 131 and pushes the second limiting structure 130 to move, thereby pushing the second limiting structure 130 to move in the first direction and the second direction at the same time, so as to determine the position of the chip 200.

In some embodiments of the present application, a pressing portion is formed on the abutment 141, and the pressing portion is arranged on the side of the abutment 141 away from the elastic member 142. Using the pressing portion, the user can apply pressure to the pressing portion so that the abutment 141 can overcome the elastic force of the elastic member 142 and rotate in a direction away from the second limiting structure 130, thereby increasing the activity space of the second limiting structure 130, which is conducive to the placement of the chip 200.

In other embodiments, the abutting member 141 may also be slidably connected to the base 110, and the elastic member 142 may allow the abutting member 141 to elastically abut against the abutting surface 131 of the second position-limiting structure 130. Under the elastic force of the elastic member 142, the abutting member 141 may slide along the first direction or the second direction and abut against the abutting surface 131, and then under the action of the abutting surface 131, the second position-limiting structure 130 is pushed to move toward the first direction and the second direction at the same time, so as to determine the position of the chip 200.

In some embodiments of the present application, the second position-limiting structure 130 forms an opening, and the opening is connected to the cavity, and the opening is used to accommodate the first position-limiting member 121 and the second position-limiting member 122. Specifically, the opening can be set to three, wherein the first position-limiting member 121 is set to two, and the second position-limiting member 122 is set to one, two openings are used to place the first position-limiting member 121, and the other opening is used to place the second position-limiting member 122. By using the mutual cooperation between the opening and the first position-limiting member 121 and the second position-limiting member 122, the chip 200 can be initially positioned, which is conducive to the smooth progress of subsequent positioning work.

In some embodiments of the present application, the chip separation and positioning device 100 further includes: a cover body 150 .

The cover 150 is rotatably connected to the base 110 . The cover 150 is used to press the chip 200 when the cover 150 and the base 110 are close to each other, so that the vertical position of the chip 200 is further determined, and the position stability of the chip 200 is further ensured.

In some embodiments of the present application, the cover 150 includes a pressing block, which is an elastic structure. When the cover 150 is in a closed state, the pressing block presses the chip 200 to determine the position of the chip 200 in the vertical direction.

The present application also provides a chip processing device, which includes the above-mentioned chip split positioning device. The above-mentioned chip split positioning device can be used to quickly position the chip, thereby improving work efficiency.

Please refer to Figures 3 to 7. The present application provides a chip seat (i.e., the chip positioning device in Figure 1) applied to a gene sequencer, including a sequencing chip platform 2 (i.e., the base 110 in Figure 1) with a built-in sequencing chip 1 (i.e., the chip 200 in Figure 1), and also includes: a pressure cover 3 (i.e., the cover body 150 in Figure 1), which is rotatably installed on the upper surface of the sequencing chip platform 2 through an axle seat or a hinge.

The chip seat includes at least two groups of pressing claw assemblies 4, which are symmetrically mounted on the lower surface of the pressing cover 3 and abut against the sequencing chip 1 to press and fix the sequencing chip 1.

Specifically, as shown in Figures 5 to 7, the pressure claw assembly 4 includes a pressure claw frame 41, a rotating shaft 42 and a pressure claw 43. Through grooves are provided on both sides of the pressure claw frame 41 to be rotatably connected to the rotating shaft 42. The pressure claw 43 abuts between the pressure claw frame 41 and the rotating shaft 42, and the pressure claw 43 is arranged in a "Z"-shaped structure. The pressure claw 43 is used to press the sequencing chip 1.

Furthermore, the pressure claw assembly 4 also includes a spring 44. One end of the pressure claw 43 is used to press the sequencing chip 1. The other end of the pressure claw 43 is fixedly connected to the pressure claw frame 41 through the spring 44 to provide pressure for the pressure claw 43 to stably press the sequencing chip 1.

Furthermore, the pressing claw assembly 4 also includes a support member 45, and the pressing claw 43 is placed between the support member 45 and the rotating shaft 42. The middle part of the pressing claw 43 abuts against the support member 45, which can make the rotation of the pressing claw 43 more stable, thereby improving the stability of the pressing claw 43.

As shown in FIG7 , the contact point between the pressure claw 43 and the support member 45 has an arc-shaped convex surface, and the pressure claw 43 can rotate along the arc-shaped convex surface to adapt to the angle of the sequencing chip 1 and can move slightly, especially when the pressure claw assembly 4 is located on the pressure cover 3 and has two or more pressure claws 43. When the sequencing chip 1 is pressed, multiple pressure claws 43 can contact the upper surface of the sequencing chip 1 at the same time, which is more compliant.

As shown in Figures 5 and 6, a limiting groove 46 is opened on the top of the pressing claw 43, and the rotating shaft 42 passes through the limiting groove 46 and is rotatably connected to the pressing claw frame 41, so as to limit the pressing claw 3 and ensure the stability during rotation, thereby improving the stability of the pressing claw 43 in pressing the sequencing chip 1.

Furthermore, the contact surface between the pressure claw 43 and the sequencing chip 1 is a flexible structure, and the flexible structure is one of a rubber pad, a sponge pad and a polyurethane foam, so as to avoid scratches between the pressure claw 43 and the sequencing chip 1, thereby protecting the sequencing chip 1.

As shown in Figures 3 and 4, the chip seat also includes at least two groups of chip positioning blocks 5 and a chip frame 6 (i.e., the second limiting structure 130 in Figure 1). The chip positioning blocks 5 are installed on the periphery of the top of the sequencing chip platform 2, and the chip frame 6 is arranged on the periphery of the sequencing chip 1 and is located in the installation area formed between the chip positioning blocks 5, which can position the sequencing chip 1, thereby improving the accuracy of detection.

As shown in FIG3 , a plurality of flow channels 7 arranged along the length direction are provided on the top of the sequencing chip 1 , and fluid ports 8 are respectively provided on both sides of the flow channels 7 along the thickness direction of the sequencing chip 1 , and the fluid ports 8 are connected to the flow channel ports 9 in the sequencing chip platform 2 to facilitate the flow of the test liquid.

As shown in FIG4 , the sequencing chip platform 2 further includes a sealing structure 11, which is located between the flow channel port 9 and the fluid port 8 and is used to seal and connect the flow channel port 9 and the fluid port 8. The sealing structure 11 is a sealing gasket or a filling sealant to prevent leakage when the test liquid circulates and improve the sealing degree.

The working principle of this embodiment is as follows: when in use, first assemble the sequencing chip 1 and the chip frame 6 and place them inside the upper surface of the sequencing chip platform 2, and fix them by the chip positioning blocks 5 located at the four corners to ensure the accuracy of the placement and the accuracy of the detection. Then, the pressure cover 3 is turned over on the upper surface of the sequencing chip platform 2, and the pressure cover 3 drives the pressure claw assembly 4 to move synchronously until the pressure claw 43 is tightly attached to the upper surface of the sequencing chip 1 to clamp and fix the sequencing chip 1. The elastic force of the spring 44 cooperates with the rotating shaft 42 and the support 45 to form a lever principle, so that one end of the pressure claw 43 gives a certain clamping force to the sequencing chip 1, keeps the entire clamping mechanism working smoothly, clamps and positions the edge of the sequencing chip 1, and the bottom surface of the pressure claw 3 corresponding to the contact with the sequencing chip 1 is prevented from scratching the sequencing chip 1 through the sealing cushion when pressing down, thereby providing protection.

Furthermore, the sequencing chip 1 presses the sealing structure 11 vertically downward through the cooperation of the spring 44 and the pressure claw 43, so that the sealing structure 11 forms a seal between the fluid port 8 and the flow channel port 9, thereby preventing leakage when the subsequent sequencing reaction working liquid passes through. At the same time, the sequencing chip 1 is positioned, and the sequencing chip 1 and internal components are protected, which is convenient for carrying and use in multiple locations.

In the solution provided in the present application, by providing a plurality of pressure claw assemblies at the bottom of the pressure cover assembly, the sequencing chip can be tightly pressed to ensure the flatness and tightness of the sequencing chip and avoid leakage; further, the sequencing chip can be positioned and the internal sequencing chip and internal components can be protected, making it convenient to carry and use in multiple locations.

Please refer to Figures 8 to 13. This embodiment provides a chip frame device for a gene sequencer, which is used to fix a sequencing chip 20 (i.e., the chip in Figure 1 and the sequencing chip 1 in Figure 3) so that the sequencing chip 20 is stably installed on the gene sequencer to improve the clarity of image acquisition. The chip frame device includes a chip frame 10 (i.e., the second limiting structure 130 in Figure 1 and the chip frame 6 in Figure 3), a sealing gasket 30, a sealing block 50 (i.e., the sealing structure 11 in Figure 4), and a chip platform 40 (i.e., the base 110 in Figure 1 and the sequencing chip 1 in Figure 3). The chip platform 2) includes a chip frame 10 which is a generally rectangular frame. The interior of the chip frame 10 is provided with a chip slot for accommodating and fixing the sequencing chip 20. The chip slot is provided with a first fixing mechanism for fixing the sequencing chip 20. Through the setting of the first fixing mechanism, the sequencing chip 20 can be stably installed in the chip slot, and at the same time, the sequencing chip 20 can be stably limited in the front-to-back, left-to-right, and up-and-down directions, thereby improving its installation fixity.

The sequencing chip 20 is provided with a plurality of transverse and mutually parallel fluid grooves 202 (i.e., the flow channels 7 in FIG. 3 ), and the lower end surface of the sequencing chip 20 is provided with fluid ports 201 connected to both ends of the fluid grooves 202. The sealing gasket 30 is detachably mounted on the bottom of the chip outer frame 10 and corresponds to the position of the fluid ports 201 to improve the sealing performance at the fluid ports 201. The sealing gasket 30 is provided with fluid circular holes 301 corresponding to the fluid ports 201 and connected to each other, so that the fluid in the fluid grooves 202 can flow into the fluid circular holes 301 in accordance with the fluid ports 201.

The chip platform 40 is used as a substrate to carry the chip frame 10, and the chip frame 10 with the sequencing chip 20 installed is fixed on the gene sequencer through the chip platform 40. A second fixing mechanism is provided between the chip frame 10 and the chip platform 40. Through the provision of the second fixing mechanism, the chip frame 10 can be stably installed on the chip platform 40, thereby improving the overall stability. The chip platform 40 is provided with a sealing block 50 corresponding to the position of the sealing gasket 30, and the sealing block 50 is provided with a flow channel opening 502 corresponding to the position of the fluid circular hole 301. When the sequencing chip 20 and the chip frame 10 are fixedly installed on the chip platform 40, the fluid groove 202, the fluid opening 201, the fluid circular hole 301 and the flow channel opening 502 are connected in sequence. When in use, the liquid flows into the chip from the sealing block on one side through the sealing gasket, and after reacting in the chip, it flows out in sequence from the sealing gasket and the sealing block on the other side through the fluid groove.

In this embodiment, preferably, a plug-in groove 303 is opened on the sealing gasket 30, and a plug-in boss 501 matching the plug-in groove 303 is fixedly installed on the sealing block 50. The sealing gasket 30 is fixedly installed at the bottom of the core outer frame and installed together on the chip platform 40. At this time, the plug-in groove 303 and the plug-in boss 501 are engaged with each other, thereby improving the positioning accuracy between the sealing gasket 30 and the sealing block 50, and ensuring the precise alignment of the fluid circular hole 301 and the flow channel opening 502.

In this embodiment, in order to improve the installation stability between the sequencing chip 20 and the chip frame 10, the first fixing mechanism specifically includes an upper limit plate 104, a lower limit plate 105 and a limit boss 103, wherein the upper limit plate 104 and the lower limit plate 105 are respectively at least one pair, and at least one pair of upper and lower limit plates 105 are respectively fixedly installed on the inner walls on the opposite sides of the chip frame 10 and the two pairs of upper and lower limit plates 105 are symmetrically distributed. In this embodiment, one pair of upper and lower limit plates 105 are fixedly installed on the front inner wall of the chip frame 10, and the other pair of upper and lower limit plates 105 are fixedly installed on the rear inner wall of the chip frame 10. A groove for upper and lower limit positioning of the sequencing chip 20 is formed between the upper limit plate 104 and the lower limit plate 105. During installation, the lower end surface of the sequencing chip 20 is supported on the lower limit plate 105, and the upper end surface thereof is in contact with the upper limit plate 104, thereby achieving the upper and lower position fixation of the sequencing chip 20. It should be noted that the upper and lower limit plates 105 may be plates extending continuously along the inner wall of the chip frame 10 , or may be a plurality of plates separated from each other and arranged at intervals, which is not specifically limited here.

The limiting boss 103 is fixedly mounted on the inner side wall of the chip outer frame 10. At least one limiting boss 103 is arranged on each inner side wall of the chip outer frame 10. The limiting boss 103 has a contact surface that conflicts with the side wall of the sequencing chip 20. The contact surface can be a plane, an arc surface or a spherical surface. During installation, the four side walls of the sequencing chip 20 are respectively in conflict with the contact surfaces on the limiting boss 103, thereby achieving multi-point limiting of the side wall of the sequencing chip 20 to achieve its position fixation in the front-back and left-right directions.

In this embodiment, preferably, the limiting boss 103 located on the front side is an elastic boss 102, and the elastic boss 102 is fixedly mounted on the inner wall of the chip outer frame 10 through an elastic sheet. The elastic sheet is roughly parallel to the inner wall of the chip outer frame 10 and has an elastic force that presses toward the inner side of the chip slot. The elastic boss 102 is fixedly mounted on the end of the elastic sheet. Through the above arrangement, the elastic boss 102 has a certain amount of interference in a free state, so that when the sequencing chip 20 is installed, the elastic force of the elastic boss 102 causes the sequencing chip 20 to be squeezed toward the opposite side of the elastic boss 102, ensuring that the sequencing chip 20 is more stable in the front and back directions. In addition, the side of the chip frame located on the elastic boss 102 is elastic. When installed, the elastic side of the chip outer frame 10 is pulled outward and a clearance space is generated. The rear side of the sequencing chip 20 is inserted between the upper and lower limiting plates 105 on the rear side, and then the front side is inserted into the front side through the clearance space. It can be between the upper and lower limit plates 105 on the side.

In this embodiment, preferably, the limiting boss 103 on the left side has an inclined surface that tilts inward from top to bottom, and the lower end of the inclined surface is transitionally connected to the contact surface on the limiting boss 103. After the sequencing chip 20 is inserted forward and backward, the sequencing chip 20 is pushed to the right, and the left side is pressed downward along the inclined surface, so that the sequencing chip 20 is smoothly buckled into the chip slot, further improving the installation convenience of the sequencing chip 20. It should be noted that in this embodiment, the inclined surface set on the limiting boss 103 on the right side can also achieve the same effect, and no specific limitation is made here.

In this embodiment, the sealing gasket 30 and the chip frame 10 are detachably connected. Specifically, a hook 101 is fixedly provided at the bottom of the chip frame 10, and a hanging hole 302 is opened on the sealing gasket 30 for the hook 101 to be inserted. The sealing gasket 30 is connected to the hook 101 at the bottom of the chip frame 10 through the hanging hole 302, thereby fixing the two.

In this embodiment, a second fixing mechanism is arranged between the chip frame 10 and the chip platform 40. Through the arrangement of the second fixing mechanism, the chip frame 10 can be stably installed on the chip platform 40, thereby improving the overall stability. Specifically, the second fixing mechanism includes at least one front-to-back positioning reference 401 (i.e., the first limiting member 121 in FIG. 1 ) and at least one left-to-right positioning reference 401 (i.e., the second limiting member 122 in FIG. 1 ) and a positioning element 402 (i.e., the execution structure 140 in FIG. 1 ). The positioning reference 401 is fixedly installed on the chip platform 40. A limiting notch 107 corresponding to the positioning reference 401 is provided on the chip frame 10. When the chip frame 10 is installed, its limiting notch 107 is aligned with the positioning reference 401, and the positioning reference 401 is embedded in the limiting notch 107, thereby realizing the positioning between the chip frame 10 and the chip platform 40 on the horizontal plane. In order to further fix the chip frame 10, the positioning element 402 and the chip platform 40 can be connected by sliding or pressing, and can be moved and switched between the retracted position and the positioning position. Taking the sliding connection of the positioning element 402 as an example, the positioning element 402 is slidably installed on the chip platform 40, and a positioning head 402a is provided on the positioning element 402. The positioning head 402a can be a plane, an arc surface or a spherical surface. When the positioning element 402 slides toward the inside of the chip slot, it can reach the positioning position, and when it slides outward, it can reach the retracted position. The outer wall of the chip frame 10 has a positioning groove 106 (that is, the groove body where the abutment surface 131 in Figure 1 is located) for the positioning head 402a to be inserted into. When the positioning element 402 slides to the positioning position, the positioning head 402a is inserted into the positioning groove 106, thereby further fixing the chip frame 10.

The chip frame assembly of the present application has a compact structure. The chip frame and the sequencing chip are fixed by a first fixing mechanism, thereby ensuring that the sequencing chip has a good stability effect in all directions of up and down, left and right, and front and back, and ensuring the accuracy and clarity of the sequencing chip image acquisition; the chip frame and the chip platform are fixed to each other by a second fixing mechanism, thereby ensuring that the overall stability of the chip frame is strong;

The chip frame assembly of the present application realizes upper and lower position limits of the sequencing chip by setting the first fixing mechanism as an upper limit plate, a lower limit plate and a limit boss, and fixing at least two pairs of upper and lower limit plates on the opposite inner walls of the chip frame. At least one limit boss with a contact surface is set on each inner side wall of the chip frame to limit the side of the sequencing chip, thereby ensuring that it is fixedly limited in the horizontal direction.

The chip frame assembly of the present application sets one of the limiting bosses as an elastic boss, and the elastic boss is fixed to the side wall of the chip frame by an elastic sheet, so that the elastic boss has a certain interference in a free state. When the sequencing chip is installed, the sequencing chip is squeezed toward the opposite side of the elastic boss under the elastic force of the elastic boss, ensuring that the sequencing chip is more stable in the front and back directions. In addition, the side of the chip frame located on the elastic boss is elastic. When installing, the elastic side of the chip frame is pulled outward and a clearance space is generated. The rear side of the sequencing chip is inserted between the upper and lower limiting plates on the rear side, and then the front side is inserted between the upper and lower limiting plates on the front side through the clearance space, which is convenient for installation.

The chip frame assembly of the present application has an inclined surface that is inclined from top to bottom toward the inside on the limiting boss on the left side, and the lower end of the inclined surface is transitionally connected with the contact surface on the limiting boss. After the sequencing chip is inserted frontward and rearward, the sequencing chip is pushed to the right, and the left side is pressed downward along the inclined surface, so that the sequencing chip is smoothly buckled into the chip slot, further improving the convenience of installing the sequencing chip.

The present application also provides a gene sequencer, which includes a chip platform for carrying chip;

A first limiting structure, fixedly connected to the chip platform, used to abut against the chip to limit the movement of the chip along a first direction and a second direction; wherein the first direction and the second direction are perpendicular to each other;

A chip outer frame, movably connected to the chip platform, the chip outer frame is provided with a chip slot, the chip is arranged in the chip slot, so as to limit the movement of the chip in a direction opposite to the first direction and a direction opposite to the second direction;

An execution structure, connected to the chip platform and abutting against the chip outer frame, used for pushing the chip outer frame to move along the first direction and the second direction to abut against the chip;

A sealing gasket, detachably mounted on the bottom of the chip frame;

The chip is provided with a plurality of transverse and mutually parallel fluid grooves, the lower end surface of the chip is provided with a fluid port connected with both ends of the fluid groove, the sealing gasket is provided with a fluid circular hole, the sealing block is provided with a flow channel port, and the fluid port, the fluid circular hole, and the flow channel port are connected in sequence. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific possible implementation scheme, the chip frame forms an abutment surface, and the abutment surface is inclined from the opposite direction of the first direction to the opposite direction of the second direction; the execution structure includes an abutment member and an elastic member; the elastic member is connected to the chip platform and the abutment member, and is used to push the abutment member to move; the abutment member abuts against the abutment surface to push the chip frame to move along the first direction and the second direction under the action of the elastic member. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific possible implementation method, the abutment member is rotatably connected to the chip platform, and the rotation axis of the abutment member is parallel to the second direction. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation scheme, one end of the elastic member is fixedly connected to the chip platform, and the other end of the elastic member is fixedly connected to one end of the abutment member, and the elastic member is used to push the abutment member to rotate toward the direction close to the chip frame. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific possible implementation scheme, a pressing portion is formed on the abutment member, and the pressing portion is arranged on a side of the abutment member away from the elastic member, and the pressing portion is used to receive an external force and rotate in a direction away from the chip frame under the action of the external force. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation scheme, the first limiting structure includes: a first limiting member fixedly connected to the chip platform and used to abut against the chip to limit the movement of the chip along the first direction; a second limiting member fixedly connected to the chip platform and used to abut against the chip to limit the movement of the chip along the second direction. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific possible implementation, the chip outer frame is annular, and a cavity for accommodating the chip is formed inside the chip outer frame; the chip outer frame has a first inner wall and a second inner wall, the first inner wall is used to abut against the chip to limit the movement of the chip in the opposite direction of the first direction; the second inner wall is used to abut against the chip to limit the movement of the chip in the opposite direction of the second direction. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation scheme, the chip frame forms an opening, the opening is connected to the cavity, and the opening is used to accommodate the first stopper and the second stopper. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation scheme, it further includes: a cover body, rotatably connected to the chip platform, for pressing the chip when close to the chip platform. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation scheme, at least two groups of pressure claw assemblies are symmetrically installed on the lower surface of the cover, and the at least two groups of pressure claw assemblies abut against the chip when the cover is in a closed state, so as to press and fix the chip. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation scheme, the pressure claw assembly includes a pressure claw frame, a rotating shaft and a pressure claw, the pressure claw frame is rotatably connected to the rotating shaft, the pressure claw abuts between the pressure claw frame and the rotating shaft, and the pressure claw is used to press the chip. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation scheme, the pressure claw assembly further includes a spring, one end of the pressure claw is used to press the chip, and the other end of the pressure claw is fixedly connected to the pressure claw frame through the spring. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific embodiment, the pressure claw assembly further includes a support member, the pressure claw is disposed between the support member and the rotating shaft, and the middle portion of the pressure claw abuts against the support member. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific embodiment, the contact point between the pressure claw and the support member has an arc-shaped convex surface, and the pressure claw can rotate along the arc-shaped convex surface to adapt to the angle of the chip. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation method, a limiting groove is provided on the top of the pressing claw, and the rotating shaft passes through the limiting groove and is rotatably connected to the pressing claw frame. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific possible implementation, the chip slot is provided with a first fixing mechanism for fixing the chip; the first fixing mechanism includes an upper limit plate and a lower limit plate, and the upper limit plate and the lower limit plate are at least one pair, and the at least one pair of upper and lower limit plates are respectively fixedly mounted on the inner walls of the opposite sides of the chip outer frame, and the chip is accommodated in the groove formed between the upper limit plate and the lower limit plate. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation scheme, the first fixing mechanism further includes a limiting boss, which is fixedly mounted on the inner side wall of the chip outer frame, and each inner side wall of the chip outer frame is provided with at least one limiting boss, which has a contact surface that conflicts with the side wall of the chip. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific possible implementation, the contact surface is a plane, a curved surface or a spherical surface. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation scheme, the limiting boss located at the front side is an elastic boss, which is fixedly mounted on the inner wall of the chip outer frame through an elastic sheet and has an elastic force to squeeze toward the inner side of the chip slot. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In a specific implementation scheme, the limiting boss on the left side has an inclined surface that tilts inward from top to bottom, and the lower end of the inclined surface is transitionally connected to the contact surface on the limiting boss. For details, please refer to the relevant descriptions in Figures 1 to 13, which will not be repeated here.

In the above technical solution, accurate fixation of the chip is achieved through the cooperation of the chip, the chip frame and the chip platform.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application. For technicians in the field of gene sequencing, the present application may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims (48)

A chip positioning device, used for positioning a chip in a gene sequencer, characterized by comprising: A base, used for carrying the chip; A first limiting structure, fixedly connected to the base, used to abut against the chip to limit the chip from moving along a first direction and a second direction; wherein the first direction and the second direction are perpendicular to each other; a second limiting structure, movably connected to the base, and used to abut against the chip to limit the movement of the chip in a direction opposite to the first direction and in a direction opposite to the second direction; The execution structure is connected to the base and abuts against the second limiting structure, and is used to push the second limiting structure to move along the first direction and the second direction to abut against the chip. The chip positioning device according to claim 1, characterized in that: the second limiting structure forms an abutment surface, and the abutment surface is inclined from a direction opposite to the first direction toward a direction opposite to the second direction; The execution structure includes an abutment member and an elastic member; the elastic member is connected to the base and the abutment member to push the abutment member to move; the abutment member abuts against the abutment surface to push the second limiting structure to move along the first direction and the second direction under the action of the elastic member. The chip positioning device according to claim 2 is characterized in that: the abutment member is rotatably connected to the base, and the rotation axis of the abutment member is parallel to the second direction. The chip positioning device as described in claim 3 is characterized in that: one end of the elastic member is fixedly connected to the base, the other end of the elastic member is fixedly connected to one end of the abutment member, and the elastic member is used to push the abutment member to rotate in a direction close to the second limiting structure. The chip positioning device as described in claim 4 is characterized in that: a pressing portion is formed on the abutment member, and the pressing portion is arranged on a side of the abutment member away from the elastic member, and the pressing portion is used to receive external force and rotate in a direction away from the second limiting structure under the action of the external force. The chip positioning device according to claim 1, characterized in that: the first limiting structure comprises: a first limiting member fixedly connected to the base and used to abut against the chip to limit the movement of the chip along the first direction; The second limiting member is fixedly connected to the base and is used to abut against the chip to limit the movement of the chip along the second direction. The chip positioning device as described in claim 6 is characterized in that: the second limiting structure is annular, and a cavity for accommodating the chip is formed inside the second limiting structure; the second limiting structure has a first inner wall and a second inner wall, the first inner wall is used to abut against the chip to limit the movement of the chip in the opposite direction of the first direction; the second inner wall is used to abut against the chip to limit the movement of the chip in the opposite direction of the second direction. The chip positioning device according to claim 7 is characterized in that: the second limiting structure forms an opening, the opening is connected to the cavity, and the opening is used to accommodate the first limiting member and the second limiting member. The chip positioning device according to any one of claims 1 to 8 is characterized in that it also includes: a cover body, rotatably connected to the base, and used to press the chip when it is close to the base. The chip positioning device as described in claim 9 is characterized in that: at least two groups of pressure claw assemblies are symmetrically installed on the lower surface of the cover body, and the at least two groups of pressure claw assemblies are in contact with the chip when the cover body is close to the base, so as to press and fix the chip. The chip positioning device as described in claim 10 is characterized in that: the pressure claw assembly includes a pressure claw frame, a rotating shaft and a pressure claw, the pressure claw frame is rotatably connected to the rotating shaft, the pressure claw abuts between the pressure claw frame and the rotating shaft, and the pressure claw is used to press the chip. The chip positioning device as described in claim 11 is characterized in that: the pressure claw assembly also includes a spring, one end of the pressure claw is used to press the chip, and the other end of the pressure claw is fixedly connected to the pressure claw frame through the spring. The chip positioning device as described in claim 12 is characterized in that: the pressure claw assembly also includes a support member, the pressure claw is placed between the support member and the rotating shaft, and the middle part of the pressure claw abuts against the support member. The chip positioning device as described in claim 13 is characterized in that: the contact point between the pressure claw and the support member has an arc-shaped convex surface, and the pressure claw can rotate along the arc-shaped convex surface to adapt to the angle of the chip. The chip positioning device as described in claim 14 is characterized in that: a limiting groove is opened on the top of the pressing claw, and the rotating shaft passes through the limiting groove and is rotatably connected to the pressing claw frame. The chip positioning device according to any one of claims 11 to 15 is characterized in that the contact surface between the pressure claw and the chip is a flexible structure, and the flexible structure is one of a rubber pad, a sponge pad and a polyurethane foam. The chip positioning device as described in claim 10 is characterized in that: a plurality of groups of flow channels arranged along the length direction of the chip are arranged inside the chip, and fluid ports along the thickness direction of the chip are respectively opened on both sides of the flow channels, and the fluid ports are connected to the flow channel ports in the base. A chip frame device for a gene sequencer, characterized in that it comprises a chip frame, a chip and a sealing gasket, wherein the chip frame is provided with a chip slot, the chip is arranged in the chip slot, and the chip slot is provided with a first fixing mechanism for fixing the chip; The sealing gasket is detachably mounted on the bottom of the chip frame; The chip is provided with a plurality of transverse and mutually parallel fluid grooves inside, the lower end surface of the chip is provided with a fluid port connected with both ends of the fluid grooves, the sealing gasket is provided with a fluid circular hole, and the fluid port and the fluid circular hole are connected in sequence. The chip frame device for a gene sequencer as described in claim 18 is characterized in that: the sealing gasket is provided with a plug-in groove for engaging with the plug-in boss of the gene sequencer. The chip frame device for a gene sequencer as described in claim 18 is characterized in that: the first fixing mechanism includes an upper limit plate and a lower limit plate, and there are at least one pair of upper limit plates and lower limit plates respectively, and at least one pair of upper and lower limit plates are respectively fixedly installed on the inner walls on opposite sides of the chip frame, and the chip is accommodated in a groove formed between the upper limit plate and the lower limit plate. The chip frame device for a gene sequencer as described in claim 20 is characterized in that: the first fixing mechanism also includes a limiting boss, and the limiting boss is fixedly mounted on the inner wall of the chip frame, and at least one limiting boss is arranged on each inner wall of the chip frame, and the limiting boss has a contact surface that conflicts with the side wall of the chip. The chip frame device for a gene sequencer as described in claim 21 is characterized in that the contact surface is a plane, a curved surface or a spherical surface. The chip frame device for a gene sequencer as described in claim 21 is characterized in that the limiting boss located on the front side is an elastic boss, which is fixedly mounted on the inner wall of the chip frame by an elastic sheet and has an elastic force that squeezes toward the inner side of the chip slot. The chip frame device for a gene sequencer as described in claim 21 is characterized in that: the limiting boss located on the left side has an inclined surface inclined from top to bottom toward the inside, and the lower end of the inclined surface is transitionally connected to the contact surface on the limiting boss. The chip frame device for a gene sequencer as described in claim 18 is characterized in that a hook is fixedly provided at the bottom of the chip frame, and a hanging hole for the hook to be inserted is opened on the sealing gasket, and the sealing gasket is connected to the hook at the bottom of the chip frame through the hanging hole. The chip frame device for a gene sequencer as described in claim 18 is characterized in that: a limiting notch corresponding to a positioning reference located on the gene sequencer is opened on the chip frame, and the positioning reference is embedded in the limiting notch. The chip frame device for a gene sequencer as described in claim 26 is characterized in that: the outer wall of the chip frame has a positioning groove for positioning and cooperating with the positioning element on the gene sequencer, and when the positioning element is in the retracted position, the positioning element is separated from the positioning groove. A gene sequencer, characterized in that it comprises: Chip platform, used to carry the chip; A first limiting structure, fixedly connected to the chip platform, used to abut against the chip to limit the movement of the chip along a first direction and a second direction; wherein the first direction and the second direction are perpendicular to each other; A chip outer frame, movably connected to the chip platform, the chip outer frame is provided with a chip slot, the chip is arranged in the chip slot, so as to limit the movement of the chip in a direction opposite to the first direction and a direction opposite to the second direction; An execution structure, connected to the chip platform and abutting against the chip outer frame, used for pushing the chip outer frame to move along the first direction and the second direction to abut against the chip; A sealing gasket, detachably mounted on the bottom of the chip frame; A sealing block corresponding to the position of the sealing pad is arranged on the chip platform; The chip is provided with a plurality of transverse and mutually parallel fluid grooves inside, the lower end surface of the chip is provided with a fluid port connected with the two ends of the fluid groove, the sealing gasket is provided with a fluid circular hole, the sealing block is provided with a flow channel opening, and the fluid port, the fluid circular hole and the flow channel opening are connected in sequence. The gene sequencer according to claim 28, characterized in that: the chip frame forms a contact surface, and the contact surface is inclined from a direction opposite to the first direction toward a direction opposite to the second direction; The execution structure includes an abutment member and an elastic member; the elastic member is connected to the chip platform and the abutment member to push the abutment member to move; the abutment member abuts against the abutment surface to push the chip frame to move along the first direction and the second direction under the action of the elastic member. The gene sequencer according to claim 29, characterized in that: The abutment member is rotatably connected to the chip platform, and a rotation axis of the abutment member is parallel to the second direction. The gene sequencer according to claim 30, characterized in that: One end of the elastic member is fixedly connected to the chip platform, and the other end of the elastic member is fixedly connected to one end of the abutment member. The elastic member is used to push the abutment member to rotate toward a direction close to the chip outer frame. The gene sequencer according to claim 31, characterized in that: A pressing portion is formed on the abutting member, and the pressing portion is arranged on a side of the abutting member away from the elastic member. The pressing portion is used to receive external force and rotate in a direction away from the chip frame under the action of the external force. The gene sequencer according to claim 28, wherein the first limiting structure comprises: A first limiting member, fixedly connected to the chip platform, and used to abut against the chip to limit the movement of the chip along the first direction; The second limiting member is fixedly connected to the chip platform and is used for abutting against the chip to limit the movement of the chip along the second direction. The gene sequencer according to claim 33, characterized in that: The chip frame is annular, and a cavity for accommodating the chip is formed inside the chip frame; the chip frame has a first inner wall and a second inner wall, the first inner wall is used to abut against the chip to limit the movement of the chip in the opposite direction of the first direction; the second inner wall is used to abut against the chip to limit the movement of the chip in the opposite direction of the second direction. The gene sequencer according to claim 34, characterized in that: The chip frame forms an opening, the opening is connected to the cavity, and the opening is used to accommodate the first limiting member and the second limiting member. The gene sequencer according to any one of claims 28 to 35, characterized in that it also includes: The cover body is rotatably connected to the chip platform and is used for pressing the chip when the cover body is close to the chip platform. The gene sequencer as claimed in claim 36 is characterized in that: at least two groups of pressure claw assemblies are symmetrically installed on the lower surface of the cover body, and the at least two groups of pressure claw assemblies are symmetrically installed on the lower surface of the cover body when the cover body is close to the chip platform. The chip is in contact with the chip in this state to press and fix the chip. The gene sequencer as described in claim 37 is characterized in that: the pressure claw assembly includes a pressure claw frame, a rotating shaft and a pressure claw, the pressure claw frame is rotatably connected to the rotating shaft, the pressure claw abuts between the pressure claw frame and the rotating shaft, and the pressure claw is used to press the chip. The gene sequencer as described in claim 38 is characterized in that: the pressure claw assembly also includes a spring, one end of the pressure claw is used to press the chip, and the other end of the pressure claw is fixedly connected to the pressure claw frame through the spring. The gene sequencer as described in claim 39 is characterized in that: the pressure claw assembly also includes a support member, the pressure claw is placed between the support member and the rotating shaft, and the middle part of the pressure claw abuts against the support member. The gene sequencer as described in claim 40 is characterized in that the contact point between the pressure claw and the support member has an arc-shaped convex surface, and the pressure claw can rotate along the arc-shaped convex surface to adapt to the angle of the chip. The gene sequencer as described in claim 41 is characterized in that a limiting groove is opened on the top of the pressure claw, and the rotating shaft passes through the limiting groove and is rotatably connected to the pressure claw frame. The gene sequencer as described in any one of claims 28 to 42 is characterized in that: a first fixing mechanism for fixing the chip is arranged on the chip slot; the first fixing mechanism includes an upper limit plate and a lower limit plate, and the upper limit plate and the lower limit plate are at least one pair respectively, and the at least one pair of upper and lower limit plates are respectively fixedly installed on the inner walls on both sides of the opposite sides of the chip frame, and the chip is accommodated in the groove formed between the upper limit plate and the lower limit plate. The gene sequencer as described in claim 43 is characterized in that: the first fixing mechanism also includes a limiting boss, and the limiting boss is fixedly installed on the inner side wall of the chip frame, and at least one limiting boss is arranged on each inner side wall of the chip frame, and the limiting boss has a contact surface that conflicts with the side wall of the chip. The gene sequencer as described in claim 44 is characterized in that the contact surface is a plane, a curved surface or a spherical surface. The gene sequencer as described in claim 44 is characterized in that the limiting boss located on the front side is an elastic boss, which is fixedly mounted on the inner wall of the chip frame by an elastic sheet and has an elastic force that squeezes toward the inner side of the chip slot. The gene sequencer as described in claim 44 is characterized in that: the limiting boss located on the left side has an inclined surface inclined inward from top to bottom, and the lower end of the inclined surface is transitionally connected to the contact surface on the limiting boss. The gene sequencer as described in claim 28 is characterized in that a hook is fixedly provided at the bottom of the chip frame, and a hanging hole for the hook to be inserted is opened on the sealing gasket, and the sealing gasket is connected to the hook at the bottom of the chip frame through the hanging hole.