WO2024258820A3 - Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase - Google Patents

Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase Download PDF

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Publication number
WO2024258820A3
WO2024258820A3 PCT/US2024/033350 US2024033350W WO2024258820A3 WO 2024258820 A3 WO2024258820 A3 WO 2024258820A3 US 2024033350 W US2024033350 W US 2024033350W WO 2024258820 A3 WO2024258820 A3 WO 2024258820A3
Authority
WO
WIPO (PCT)
Prior art keywords
xylosidase
beta
processes
fermentation products
producing fermentation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2024/033350
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French (fr)
Other versions
WO2024258820A2 (en
Inventor
Aidin BALO
Chee-Leong Soong
Xiangyu CAI
Yueming SUN
Ye Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
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Novozymes AS
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Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Priority to EP24739342.4A priority Critical patent/EP4728086A2/en
Priority to MX2025015096A priority patent/MX2025015096A/en
Priority to AU2024303961A priority patent/AU2024303961A1/en
Publication of WO2024258820A2 publication Critical patent/WO2024258820A2/en
Publication of WO2024258820A3 publication Critical patent/WO2024258820A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to processes of producing fermentation products, such as ethanol from starch-containing material using fermenting organisms that express a GH120 or GH3 beta-xylosidase.
PCT/US2024/033350 2023-06-13 2024-06-11 Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase Ceased WO2024258820A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP24739342.4A EP4728086A2 (en) 2023-06-13 2024-06-11 Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
MX2025015096A MX2025015096A (en) 2023-06-13 2024-06-11 PROCESSES FOR PRODUCING FERMENTATION PRODUCTS USING MODIFIED YEASTS THAT EXPRESS A BETA-XYLOSIDASE
AU2024303961A AU2024303961A1 (en) 2023-06-13 2024-06-11 Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363507960P 2023-06-13 2023-06-13
US63/507,960 2023-06-13

Publications (2)

Publication Number Publication Date
WO2024258820A2 WO2024258820A2 (en) 2024-12-19
WO2024258820A3 true WO2024258820A3 (en) 2025-01-23

Family

ID=91829243

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/033350 Ceased WO2024258820A2 (en) 2023-06-13 2024-06-11 Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase

Country Status (4)

Country Link
EP (1) EP4728086A2 (en)
AU (1) AU2024303961A1 (en)
MX (1) MX2025015096A (en)
WO (1) WO2024258820A2 (en)

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US20220279818A1 (en) * 2019-08-05 2022-09-08 Novozymes A/S Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct

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