WO2025007130A2 - Réseau de microélectrodes souple, biorésorbable et transparent pour applications cardiaques - Google Patents

Réseau de microélectrodes souple, biorésorbable et transparent pour applications cardiaques Download PDF

Info

Publication number
WO2025007130A2
WO2025007130A2 PCT/US2024/036401 US2024036401W WO2025007130A2 WO 2025007130 A2 WO2025007130 A2 WO 2025007130A2 US 2024036401 W US2024036401 W US 2024036401W WO 2025007130 A2 WO2025007130 A2 WO 2025007130A2
Authority
WO
WIPO (PCT)
Prior art keywords
cardiac
mea
bioresorbable material
bioresorbable
cardiac device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2024/036401
Other languages
English (en)
Other versions
WO2025007130A3 (fr
Inventor
Luyao Lu
Igor R. Efimov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
George Washington University
Original Assignee
George Washington University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by George Washington University filed Critical George Washington University
Publication of WO2025007130A2 publication Critical patent/WO2025007130A2/fr
Publication of WO2025007130A3 publication Critical patent/WO2025007130A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N5/0613—Apparatus adapted for a specific treatment
    • A61N5/0622—Optical stimulation for exciting neural tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/24—Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
    • A61B5/25—Bioelectric electrodes therefor
    • A61B5/279—Bioelectric electrodes therefor specially adapted for particular uses
    • A61B5/28—Bioelectric electrodes therefor specially adapted for particular uses for electrocardiography [ECG]
    • A61B5/283—Invasive
    • A61B5/287—Holders for multiple electrodes, e.g. electrode catheters for electrophysiological study [EPS]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/24—Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
    • A61B5/25—Bioelectric electrodes therefor
    • A61B5/279—Bioelectric electrodes therefor specially adapted for particular uses
    • A61B5/28—Bioelectric electrodes therefor specially adapted for particular uses for electrocardiography [ECG]
    • A61B5/283—Invasive
    • A61B5/29—Invasive for permanent or long-term implantation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/04—Electrodes
    • A61N1/05—Electrodes for implantation or insertion into the body, e.g. heart electrode
    • A61N1/0587—Epicardial electrode systems; Endocardial electrodes piercing the pericardium
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N5/0601—Apparatus for use inside the body

Definitions

  • MAAs Microelectrode arrays
  • optically transparent MEAs are highly desired during clinical processes to allow 7 direct observation of areas of interest under the microelectrode sites for concurrent optical diagnostics/therapies (e.g.. endoscopy) and guiding other procedures (e.g., catheters) on the hearts.
  • all current transparent MEAs are designed to exhibit long-term reliable performance for chronic biointerfacing.
  • Bioresorbable is any material that can be absorbed by the body. Bioresorbable materials are used in medical applications, such as sutures, so that they do not need to be manually removed from the body. They typically degrade over a period of time.
  • Bioresorbable electronics offer unique opportunities to investigate, monitor, and treat short-lived cardiac complications, such as postoperative arrhythmias and heart failure on the order of a few days to weeks following ischemic events or surgery, which account for at least one-third of postoperative deaths.
  • Those devices can subsequently dissolve into benign products via natural metabolic mechanisms to avoid complications from surgical retrieval of the implants, lower infection risks, and eliminate additional financial burdens to patients. For example, removal of the temporary pacing devices following completion of therapy can cause laceration and perforation of the myocardium.
  • soft bioresorbable electronic devices There has been much recent work on soft bioresorbable electronic devices.
  • Examples include cardiac pacemakers to treat cardiac arrhythmias, peripheral nerve stimulators for pain block, electrical sensors to map activity from the cerebral cortex or record physiological signals, electrotherapy systems to provide electrostimulation and impedance sensing, chemical sensors to monitor critical biomarkers in various organs, etc. See Y. S. Choi. R. T. Yin, A. Pfenniger, J. Koo, R. Avila, K. Benjamin Lee, S. W. Chen, G. Lee, G. Li, Y. Qiao, A. Murillo-Berlioz, A. Kiss, S. Han, S. M. Lee, C. Li, Z. Xie, Y.-Y. Chen, A. Burrell, B. GmbH, H. Jeong, J.
  • the present disclosure provides matenals, device designs, fabrication strategies, performance characteristics, ex vivo and in vivo demonstrations, and systematic biocompatibility assessments of a fully bioresorbable, implantable, flexible, and transparent MEA technology that can provide organ conformal cardiac interfacing over clinically relevant temporary timescales.
  • the devices integrate entirely Food and Drug Administration approved materials that will completely disappear through natural biological processes in the body after a desired period of use.
  • FIGS. 1A-1H show a soft, bioresorbable, and transparent MEA for multimodal electrical and optical cardiac interrogation.
  • FIG. 1A is a schematic illustration of a MEA on a heart.
  • the device comprises Mo nanogrid microelectrodes and interconnects.
  • FIG. IB are optical images of the MEA to show transparency (left) and flexibility (right).
  • FIG. 1C is a scanning electron microscopy (SEM) image of the Mo nanogrid structure. Grid width: 500 nm. Grid thickness: 1000 nm.
  • FIG. ID is a graph of a transmission spectra of the Mo nanogrids (pitch: 6.75 pm) at various thicknesses.
  • FIG. IE is a graph of a transmission spectra of the Mo nanogrids (thickness: 1000 nm) at various pitch values.
  • FIG. IF shows dissolution kinetics of 1000 nm-thick Mo layers in lx PBS at 37, 60, and 90 °C, evaluated as decreases in thicknesses.
  • FIGS. 1G, 1H are optical images of the MEA device at various dissolution stages in l x PBS at 37 °C.
  • FIGS. 2A-2I show electrochemical characterization of Mo nanogrid microelectrodes and ME As.
  • FIG. 2A shows impedance spectra of Mo nanogrid microelectrodes (pitch: 6.75 pm) at various thicknesses.
  • FIG. 2B shows impedance spectra of Mo nanogrid microelectrodes (thickness: 1000 nm) at various pitch values.
  • FIG. 2C shows 1 kHz impedance values of Mo nanogrid microelectrodes as a function of microelectrode sizes.
  • FIG. 2D is a phase plot of the Mo nanogrid microelectrode.
  • FIG. 2E shows normalized impedance versus transmittance comparison of different transparent microelectrode materials for electrophysiological studies where the Mo nanogrid microelectrode is the only bioresorbable one.
  • FIG. 2F shows CV curves of a Mo nanogrid microelectrode at various scan rates.
  • FIG. 2G shows impedance spectra of all 16 Mo nanogrid microelectrodes in a MEA. Inset: 1 kHz impedance colormap of the microelectrodes relative to the actual microelectrode position in the MEA.
  • FIG. 2H is a CSC c histogram of 16 Mo nanogrid microelectrodes in a MEA at the scan rate of 50 mV/s. Inset: CSCc colormap of the microelectrodes relative to the actual microelectrode position in the MEA.
  • FIG. 21 shows impedance of a Mo nanogrid MEA as a function of bending cycles at 1 cm radius. Z and Zo represent the impedance value at a specific bending cycle and the initial impedance, respectively.
  • FIGS. 3A-3F show benchtop measurements of Mo nanogrid microelectrodes and MEAs.
  • FIG. 3A is a representative output recording of a Mo nanogrid microelectrode during a 10 Hz, 20 mV peak-to-peak amplitude sine wave input delivered by a Pt electrode in PBS.
  • FIG. 3B is a representative PSD curve of the recorded signals in (A).
  • FIG. 3C is a histogram of calculated SNRs of 16 microelectrodes in a MEA. Inset: SNR colormap of the microelectrodes relative to the actual microelectrode position in the MEA.
  • FIG. 3D is a histogram of calculated RMS noises of 16 microelectrodes in a MEA. Inset: RMS noise colormap of the microelectrodes relative to the actual microelectrode position in the MEA.
  • FIG. 3E shows changes in impedance (black line) and CSCc (red line) during dissolution in PBS at 37 °C.
  • Z and CSC c represent the impedance and CSCc value at a specific day while Zo and (CSCc)o represent the initial impedance and CSCc, respectively.
  • FIG. 3F are impedance curves and equivalent circuit model fittings of the MEAs at different dissolution stages on Day 0 and 4, respectively.
  • FIGS. 4A-G are an Ex vivo demonstration of the MEA for simultaneous co-localized electrical/optical interrogation of rat hearts and human ventricular slices.
  • FIG. 4A Top is a schematic illustration of EG mapping (from the MEAs) and optical dual mapping (Vm and Ca 2+ ) system. Bottom is a schematic illustration of simultaneous multi-level and multiparametric mapping in the same field of view.
  • FIG. 4B is a synchronous and co-localized EG, Vm, and Ca 2+ traces recorded from different cardiac tissue regions beneath the transparent microelectrodes of the MEA during sinus rhythm.
  • Green box Zoomed in view of three overlapping traces at each microelectrode of the MEA.
  • FIG. 4C are concurrent Vm (left), EG (middle) and Ca 2+ (right) activation maps during sinus rhythm.
  • the white square represents the region covered by the transparent MEA.
  • FIG. 4D are concurrent Vm (left), EG (middle) and Ca 2+ (right) activation maps during MEA pacing.
  • the pacing site is marked with yellow lightning bolts.
  • FIG. 4E are QRS complexes (black line) recorded by the MEAs at various dissolution stages in PBS at 37 °C, and correlated SNRs (red symbol). The QRS complexes are recorded from the same rat heart.
  • FIG. 4F Left is a schematic illustration of the region where the human ventricular slices are collected. Right is an optical image of the MEA on a human cardiac slice to show transparency.
  • FIG. 4G is a camera view of the MEA on a human cardiac slice (left), V m (middle) and EG (right) activation maps.
  • FIGS. 5A-5F show an In vivo demonstration of the MEA for simultaneous electrical monitoring and pacing for arrhythmias management.
  • FIG. 5A is an optical image of the MEA contacting the left ventricle of a blood- perfused rat heart to show transparency and flexibility.
  • FIG. 5B Left is a schematic illustration of the electrical mapping location of the MEA on a rat heart (front-lateral side of the left ventricle). Right is an electrical activation map generated by the MEA during sinus rhythm.
  • FIG. 5C are electrical activation maps from the MEA during unipolar pacing at different sites.
  • the pacing sites are marked with yellow lightning bolts.
  • the red arrow 7 represents the direction of cardiac wave propagation.
  • FIG. 5D is an electrical activation map from the MEA during bipolar pacing with two neighboring microelectrodes in the MEA.
  • FIG. 5E is a demonstration of MEA multi-channel sensing and pacing capabilities for arrhy thmias monitoring and treatment.
  • Orange box Simultaneous EG recording and bipolar pacing from the MEA during 3: 1 AV block.
  • Green box Zoomed in view of the 3: 1 AV block recorded by far-field ECG. The location of AV block (cartoon).
  • FIG. 5F show QRS complexes (black line) recorded by the bioresorbable MEAs at various dissolution stages in PBS at 37 °C, and correlated SNRs (red symbol).
  • the QRS complexes are recorded from the same rat heart.
  • FIGS. 6A-6J show an In vivo bioresorption, histology, biocompatibility, and serology test.
  • FIG. 6A are optical images of the rat hearts in situ (square box) and after explantation (circular box).
  • FIG. 6B are representative histology images of Masson’s trichrome staining of cardiac tissues. Red: Cardiomyocytes staining. Blue: Fibrosis staining. White: Interstitial space staining. Devices are stitched over the epicardium (the green triangle indicates the epicardial side).
  • FIG. 6C are quantified volume percentage of the cardiomyocytes, fibrosis, and interstitial space in FIG. 6B. No statistically significant difference is found among different groups.
  • FIG. 6D is a transthoracic echocardiogram test on the stroke volume and ejection function of the heart at different time points after implantation. No statistically significant difference is found.
  • FIG. 6E is a weight measurement of the animals at different time points after implantation. A normal weight loss is observed immediately after the surgery and is followed by a gradual weight gain resulting from a natural increase in age.
  • Serology test of biomarkers for the evaluation of the function of heart (FIG. 6F), liver (FIG. 6G), kidney (FIG. 6H), and the level of inflammation (FIG. 61) and electrolyte (FIG. 6J). No statistically significant difference is found among different groups. Data are presented with error bars as means ⁇ S.D. The statistical comparison among different groups is calculated with a nonparametric Kruskal -Wallis test in conjunction with Dunn’s multiple comparison tests at a significance level of P ⁇ 0.05. n 3 animals per group.
  • FIG. 7 is a schematic illustration of the fabrication process for the soft, bioresorbable, transparent Mo nanogrid MEA.
  • FIGS. 8A-8C show a soft, bioresorbable, transparent Mo microgrid microelectrodes via photolithography.
  • FIG. 8A is an optical image of Mo microgrids on PLGA with a grid width of 8 pm and pitch of 144 pm.
  • FIG. 8B is a representative transmittance spectrum for FIG. 8A.
  • FIG. 8C is an impedance curve of the Mo microgrid microelectrodes in FIG. 8A.
  • FIG. 9 are SEM images of the Mo nanogrids on PLGA substrates at different dissolution stages (Week 0, 2 and 5).
  • FIGS. 10A. 10B are HRTEM images.
  • FIG. 10A show EDS spectra of Mo.
  • FIG. 10B show at Day 0, 1, and 2, respectively.
  • FIGS. 11A-11D are a representative benchtop electrical output recording of 10 Hz sine waves at various amplitudes in PBS by the Mo nanogrid microelectrodes.
  • the output recording from sine wave inputs at the peak-to-peak amplitude of (FIG. 11 A) 2 mV, (FIG. 1 IB) 1 mV, (FIG. 11C) 200 pV, (FIG. 1 ID) 100 pV.
  • FIG. 12 is a 10 Hz impedance histogram of 16 Mo nanogrid microelectrodes in a MEA. Inset: 10 Hz impedance colormap of the microelectrodes relative to the actual microelectrode position in the MEA.
  • FIGS. 13A-13H show SNRs and RMS noises of 4 additional MEAs.
  • FIGS. 13A- 13D Histograms of calculated SNRs of 64 microelectrodes in 4 additional MEAs. Inset: SNR colormap of the microelectrodes relative to the actual microelectrode position in the MEA.
  • FIGS. 13E-13H Histograms of calculated RMS noises of 64 microelectrodes in 4 additional MEAs. Inset: RMS noise colormap of the microelectrodes relative to the actual microelectrode position in the MEA.
  • FIG. 14 is an equivalent circuit model for Mo nanogrid microelectrode EIS simulation.
  • FIG. 15 shows MEA activation maps during AV block without and with bipolar pacing using the MEA.
  • FIGS. 16A-16D are representative QRS complexes recorded by gold microelectrodes on Langendorff perfused rat hearts during sinus rhythm.
  • FIGS. 17A-17F are an echocardiogram assessment of cardiac relaxation and contraction function of animals following the MEA implantation.
  • FIG. 17A shows diastolic volume is the volume of blood in the left ventricle at the end of ventricular filling.
  • FIG. 17B shows systolic volume is the volume of blood in the left ventricle at the end of ventricular contraction.
  • FIG. 17C shows cardiac output is the volume of blood pumped out by the left ventricle per minute.
  • FIG. 17D shows diastolic diameter is the diameter across the left ventricle at the end of diastole.
  • FIG. 17E shows systolic diameter is the diameter across the left ventricle at the end of systole.
  • fractional shortening (diastolic diameter - systolic diameter) / diastolic diameter).
  • FIGS. 18A-18B show a serology test for liver function assessment.
  • FIG. 18A shows analysis of total bilirubin and
  • FIGS. 20A, 20B show a mechanical bending test system.
  • FIG. 20A is an optical image of the motorized test stand.
  • FIG. 20B is a zoomed in image of a device on the test machine.
  • FIG. 21 is a system for electrochemical characterizations.
  • FIG. 22 is a system for optical mapping of the human cardiac tissue slices.
  • FIG. 1A is a non-limiting illustrative embodiment of a medical detection and treatment device 10. and more specifically a microelectrode array (MEA) device or assembly 10, in accordance with an example embodiment of the present disclosure.
  • the assembly 10 has a substrate layer 100. an active layer 200, and an encapsulation layer 300.
  • the entire assembly 10 is bioresorbable, wherein the substrate layer 100, active layer 200, and encapsulation layer 300 are each made of a bioresorbable material, so that the assembly 10 is dissolved or absorbed by the body after a period of time, and need not be manually removed from the body.
  • the entire assembly 10 is optically transparent.
  • the bioresorbable material for each of the substrate layer 100, active layer 200, and encapsulation layer 300 is transparent. And, the entire assembly 10 is flexible so that the assembly 10 can bend in half so that opposite ends come together. Accordingly, the bioresorbable material for each of the substrate layer 100, active layer 200, and encapsulation layer 300 is flexible.
  • the substrate layer 100 is optically transparent and flexible, and is made of a bioresorbable material.
  • the substrate 100 retains and provides structural support to the active layer 200, and so can be thicker (thickness: 90 pm) than the encapsulation layer 300.
  • the bioresorbable substrate material is polylactic-co-gly colic acid (PLGA).
  • the active layer 200 includes the electronic components of the assembly 10, and is coupled to the substrate layer 100.
  • the electronic components include a metal grid 210, and an array of one or more electrodes 220.
  • the grid can optionally have a structural purpose to hold the electrodes in position, and an electrical purpose to communicate signals to/from the electrodes.
  • the electrodes 220 can form an integral part of the metal grid 210, and in other embodiments the electrodes 220 can be separate components and coupled (e.g., a fastener) or affixed (e.g., adhered) to the grid 210.
  • the grid can have support bars that are arranged in columns and rows with spaces/voids therebetween and form a square, rectangular, or other shape.
  • One or more interconnects 230 are provided, which can be serpentine or other shape.
  • the interconnects 230 can electrically couple the electrodes 220 and/or grid 210 to an external electronic component, such as a signal regulator or signal analyzer, and extends from the grid 210 or electrode 220 to an electrical pad on the substrate 100 or at the edge of the substrate 100.
  • the interconnects 230 carry electrical signals used to apply a therapy or treatment to the electrode 220 and/or to detect an electrical signal sensed by the electrode 220.
  • the interconnects 230 can optionally be flexible, transparent, bioresorbtive, and can be metal.
  • the interconnect 230 can be sufficiently thin to dissolve or pass out of the body once the other layers 100, 300 dissolve.
  • One or more electrical pads are provided.
  • the active layer 200 can be formed in any suitable manner, such as an electron-beam lithography (EBL) patterned transparent molybdenum (Mo) nanogrid MEA, and a photolithography determined interconnect layer.
  • EBL
  • the encapsulation layer 300 is formed over the active layer 200, so that the active layer 200 is fixedly positioned between the substrate 100 and the encapsulation layer 300.
  • the encapsulation layer 300 can be formed by a soft lithography defined transparent PLGA and can be thinner (thickness: 40 pm) than the substrate layer 100.
  • the substrate 100 can be the same or smaller thickness than the encapsulation layer 300, and the substrate 100 and encapsulation layer 300 need not both be made of the same material.
  • the encapsulation layer 300 and substrate 100 provide a liquid-proof and touch-proof seal for the active layer 200, so that liquid does not interfere with operation of the active layer 200 and the active layer does not come into contact with the body.
  • the substrate 100 and encapsulation layer 300 do not interfere with the operation of the active layer 200, so that for example the electrodes 200 can detect signals from the body and apply therapeutic signals to the body.
  • the assembly 10 can be prepared in any suitable manner, including that the nanoscale Mo structures can be based on metal grid approaches for preparing high-performance soft transparent MEAs, such as shown for example, in S. N. Obaid, R. T. Yin, J. Tian, Z. Chen, S. W. Chen, K. B. Lee, N. Boyajian, A. N. Miniovich, I. R. Efimov, L. Lu, Multifunctional Flexible Biointerfaces for Simultaneous Colocalized Optophysiology and Electrophysiology. Adv. Fund. Mater. 30, 1910027 (2020); S. N. Obaid, Z. Chen, M. Madrid, Z. Lin, J. Tian, C. Humphreys, J. Adams, N.
  • the MEA assembly 10 enables both single site and multisite pacing with simultaneous electrical cardiac mapping in detecting and treating cardiac complications, require no genetic labels, and avoid the potential optical crosstalk with co-localized optical mapping investigations, which are more suitable for future temporary non-toxic safe use in humans.
  • the MEAs are stable for several days when immersed in phosphate-buffered saline (PBS), which is on par with many postoperative care cycles, followed by complete bioresorption via hydrolysis within 6 weeks in vivo.
  • PBS phosphate-buffered saline
  • FIG. 1A shows one non-limiting illustrative example embodiment of the assembly 10.
  • the assembly 10 can be a plurality of microelectrodes 220 formed as a 4 x 4 array 225 that is attached to a nanogrid 210.
  • the microelectrodes 220 are bidirectional and the array 225 is attached to the heart surface in any suitable manner, such a with bioresorbable sutures.
  • the MEA device is optically transparent (FIG. IB, left) and bendable / flexible (FIG. IB, right).
  • Each microelectrode features a nanogrid network structure with a thickness of 1000 nm, a grid width of 500 nm, and a pitch of 6.75 pm (FIG. 1C).
  • the nanoscale dimensions enable adjusting the physical properties of the nanogrid network when scaling down the microelectrode sizes.
  • the void spaces between the nanogrids allow photons to pass through the microelectrodes 220 for co-localized optical operations.
  • the edge-to-edge distance between two nearby microelectrodes 220 in the MEA 225 is determined by the electrophysiological space constant of cardiac tissue to achieve high spatial resolution electrical mapping and control of the cardiac signal propagation from the epicardium.
  • the overall active MEA area ( ⁇ 5 x 5 mm 2 ) is on par with the sizes of ventricles or atria of small animals.
  • the entire device is thin (thickness -140 pm) and extremely lightweight (-16 mg).
  • One key feature of the MEA of the present disclosure is that it consists entirely of bioresorbable and biocompatible materials that eventually dissolve in the body.
  • the microelectrodes 220 and interconnects 230 rely on Mo metal due to its relatively slow dissolution rate under physiological conditions, which could help to extend the device’s operational lifetime compared to other metals such as magnesium.
  • PLGA lactide:glycolide 65:35
  • PLGA lactide:glycolide 65:35
  • the fabrication process appears in FIG. 7 and is discussed in the Materials and Methods section below, which shows a high yield of 97% (n >40 MEA devices).
  • the fabrication process for the bioresorbable transparent MEA is versatile and also compatible with the standard photolithography process, which can generate microscale grids with programmable parameters if needed (FIGS. 8A-8C).
  • the devices are bioresorbable transparent microelectrode arrays that allow multimodal investigation of the spatiotemporal cardiac characteristics to study and treat heart disease over clinically relevant temporary timescales and then fully dissolve inside the body without the need for additional surgical extraction.
  • Our devices establish the foundations of a soft bioresorbable transparent MEA technology to greatly expand the landscape for bioresorbable transient electronics, with the potential to address the unmet needs in fundamental and translational cardiac research (e.g., ablation and surgical intervention procedures, postoperative recovery monitoring, post-infarction recovery, post-transcatheter aortic valve replacement (TAVR) recovery) where transient mapping and control of cardiac physiological parameters and functions are required.
  • the devices represent the first optically transparent and bioresorbable microelectrode technology for cardiac applications. No devices with such capabilities exist before.
  • the active layer 200 can be one or more conductors, such as electrodes 220.
  • the active layer 200 can communicate by wire to an external device, such as an external processing device.
  • the active layer can communicate wirelessly, such as by the active layer including a first coil and a second coil is placed external to the patient to perform wireless communication and/or inductive power transfer.
  • the active layer detects cardiac conditions and transmits detection signals with the detected information to the external processing device, which can then generate reports or data, including for example electronic and/or optical mapping.
  • the present disclosure can conduct processing and mapping in any suitable manner, such as in U.S. Patent Publication Nos. 2018/0235692 and 2023/0085578, which are hereby incorporated by reference.
  • the external processing device can perform various functions and operations in accordance with the disclosure.
  • the processing device can be, for instance, a computer, personal computer (PC), server or mainframe computer, or more generally a computing device, processor, application specific integrated circuits (ASIC), or controller.
  • the processing device can execute software that can be stored on the storage device.
  • the substrate layer 100 is a thin sheet.
  • the substrate 100 is flat with a planar (though bendable) top surface and planar (though bendable) bottom surface (that faces and optionally touches the body (heart)).
  • the active layer (e.g., nanogrid microelectrode array) 200 is placed over the substrate 100 and adhered to the top surface of the substrate 100 due to their high binding strength.
  • the encapsulation layer 300 is then placed over the active layer and affixed to the substrate so that the encapsulation layer and substrate fully enclose the active layer sandwiched between the substrate layer and the encapsulation layer 300.
  • the encapsulation layer 300 can have the same size and shape as the substrate layer 100.
  • the substrate layer 100 and the encapsulation layer 300 can have a similar shape (or a different shape) as the active layer 200, and are larger than the active layer 200 to fully enclose the active layer 200 and so the combined substrate layer 100 and encapsulation layer 300 can be reliably affixed to the heart, such as by sutures. Or an adhesive can be placed on the bottom surface of the substrate layer 100. It is further noted that the period in which the bioresorbable materials dissolve can vary depending on the thickness of the materials.
  • the microelectrode arrays are transparent. This feature will allow photons to pass through both directions to allow direct observation of areas of interest under the microelectrode sites for concurrent optical diagnostics/therapies (e.g., endoscopy) and guiding other procedures (e.g., catheters) on the hearts. It will also enable optical measurement of important cardiac parameters such as metabolic activity or intracellular calcium dynamics at locations underneath the microelectrodes using microscopes or cameras.
  • the optical signals could be from intrinsic autofluorescent biomarkers in cells or external fluorescent indicators, which are not possible to measure electrically. Accordingly, for example, an optical layer can be placed over the device to transmit and receive optical signals to/from the patient’s heart.
  • microelectrode arrays provide electrical mapping of cardiac dynamics and on-demand electrical pacing to treat cardiac dysfunctions. Together, this will enable crosstalk-free and multiparametric investigation and treatment of heart diseases combining multiple modalities.
  • the microelectrode arrays detect cardiac conditions and transmit those to a remote processing device for diagnostics and analysis, and also are used to apply a treatment pulse to the heart.
  • FIG. ID presents the transmission spectra of Mo nanogrids (grid width: 500 nm, pitch: 6.75 pm) in the visible wavelength range at various thicknesses. As the nanogrid thickness increases from 100 to 500 and 1000 nm, the average transmittance value at 550 nm first decreases from 84.2 ⁇ 1.0% to 80.0 ⁇ 1.5% and then stabilizes at 79.1 ⁇ 1.2%, respectively.
  • FIG. IE shows the effect of nanogrid pitch (grid width: 500 nm, thickness: 1000 nm) on optical transparency.
  • the average 550 nm transmittance value increases from 53.5 ⁇ 2.0% to 79.1 ⁇ 1.2%, and 85.4 ⁇ 1.1% when the pitch increases from 3.5 to 6.75, and 14.75 pm, respectively.
  • the bioresorbable Mo nanogrid microelectrodes exhibit excellent optical transparency to allow light transmission in both directions.
  • FIG. IF demonstrates the dissolution kinetics and thickness profiles of the 1000 nm-thick Mo layers in l x PBS (pH 7.4).
  • the measured dissolution rate is 2.4, 19, and 62 nm/h at 37, 60 and 90 °C, respectively.
  • ⁇ 0.3 pg of Mo dissolves per day, which is within the safe daily intake (-45 pg).
  • FIGS. 1G, 1H demonstrate the different dissolution stages of the MEA device immersed in 1 x PBS (pH 7.4, 37 °C).
  • the PLGA encapsulation and support layers dissolve by hydrolysis into its monomers, lactic acid, and glycolic acid.
  • the results show that the MEA completely dissolves after 25 weeks.
  • SEM measurements further illustrate the changes in surface morphologies at different soaking periods, revealing that the Mo nanogrids on the PLGA substrate gradually develop cracks due to the swelling of the substrate induced by PLGA dissolution, and most of the nanogrid patterns disappear from the MEA region after 5 weeks (FIG. 9).
  • High-resolution transmission electron microscopy (HRTEM) and energy dispersive X-ray spectroscopy (EDS) measurements show that the lattice structures of Mo remain unchanged at initial stages of soaking (the interplanar spacing is 0.21, 0.21, and 0.22 nm at Day 0, 1, and 2) while the oxygen content relative to Mo increases gradually in the devices.
  • Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measurements evaluate the recording and stimulation capability of the Mo nanogrid MEA.
  • EIS Electrochemical impedance spectroscopy
  • CV cyclic voltammetry
  • FIG. 2A displays the impedance curves of Mo nanogrid microelectrodes at various thicknesses (grid width: 500 nm, pitch: 6.75 pm).
  • the 1 kHz impedance decreases from 44.2 ⁇ 2.8 to 23.9 ⁇ 0.9 and 13.8 ⁇ 0.8 kilohms as the Mo nanogrid thickness increases from 100 to 500 and 1000 nm, respectively.
  • FIG. 2B shows the impedance curves of the microelectrodes with various pitch values (grid width: 500 nm, thickness: 1000 nm).
  • the 1 kHz impedance increases from 6.2 ⁇ 0.4 to 13.8 ⁇ 0.8 and 33.9 ⁇ 1.5 kilohms as the pitch increases from 3.5 to 6.75, and 14.75 pm, respectively.
  • 2C depicts the linear dependence of 1 kHz impedance on the microelectrode sizes from 500 x 500 to 100 x 100 pm 2 , indicating the presence of a capacitive interface between the Mo nanogrid microelectrode and the electrolyte solution.
  • phase angles of the microelectrode (-82.1° to -71.5°) at physiologically relevant low frequencies (10 Hz to 1 kHz) further confirm the capacitive electrode/electrolyte interface (FIG. 2D).
  • the Mo nanogrid MEAs with a grid width of 500 nm, a pitch of 6.75 pm, and a thickness of 1000 nm are used in subsequent characterizations.
  • FIG. 2E compares the normalized 1 kHz impedance of the microelectrodes to state-of-the-art non-bioresorbable transparent microelectrode material candidates with comparable optical transparency (-80%) for electrophysiological studies, including indium tin oxide (ITO), Au nanogrid, CNTs, graphene, and poly(3,4- ethylenedioxythiophene):polystyrene sulfonate (PEDOT PSS).
  • ITO indium tin oxide
  • Au nanogrid Au nanogrid
  • CNTs carbon nanogrid
  • graphene graphene
  • PEDOT PSS poly(3,4- ethylenedioxythiophene):polystyrene sulfonate
  • the Mo nanogrid microelectrodes possess one of the most competitive electrochemical performances.
  • FIG. 2F shows the CV curves of Mo nanogrid microelectrodes from -1.0 to -0.1 V at various scan rates from 20 to 200
  • CSCc Cathodic charge storage capacity
  • FIG. 2G presents the impedance responses of the 16 microelectrodes in a Mo nanogrid MEA. exhibiting an average 1 kHz impedance value of 14.2 ⁇ 2.7 kilohms.
  • the inset impedance colormap in FIG. 2G displays the spatial distribution of the 1 kHz impedance from the MEA to better visualize the uniform performance.
  • FIG. 2H demonstrates the highly uniform CSCc values of all 16 microelectrode channels.
  • FIG. 21 shows that the MEAs maintain a stable electrochemical performance after 5000 bends against a small radius of 1 cm, suggesting the excellent mechanical compliance of the devices.
  • the flexible mechanics is crucial to form a conformal contact with the curvilinear heart surfaces.
  • the MEAs demonstrate highly uniform SNR and root mean square (RMS) noise, with an average SNR and RMS noise of 43.7 ⁇ 1.7 dB and 36.3 ⁇ 2.0 pV. respectively (FIGS. 3C, 3D). More SNR and RMS noise results from 64 other microelectrodes in 4 additional MEAs are available in FIGS. 13A-13H. Together, those measurements highlight the uniform performance for the microelectrodes both within the same MEA and across different MEAs in recording physiologically relevant signals.
  • RMS root mean square
  • FIG. 3E presents average changes in the impedance (black line) and CSCc (red line) from the microelectrodes over time. As expected, the impedance gradually increases, and CSCc gradually decreases due to the dissolution of Mo in PBS. The microelectrodes still exhibit moderate 1 kHz impedance (223.4 kilohms) and CSCc (0.71 mC/cm 2 ) on Day 4.
  • FIG. 14 Fitting the EIS results before and after resorption to an equivalent circuit model (FIG. 14) provides additional insights into the electrochemical behavior at the microelectrode-tissue interface.
  • the model comprises solution resistance (R s ), the resistance of the oxide layer (Roxtde , constant phase element (CPE), charge-transfer resistance (Ret) of the electrochemical corrosion process, and Warburg impedance (Zw) for ion diffusion.
  • a parallel capacitance (CL) and resistance (RL) are added in series to fit the impedance data at high-frequency domains due to the charge transfer process at the exterior surface of the microelectrode.
  • the data from Day 0 and Day 4 closely match the fitting model (FIG. 3F).
  • Yo, J. (o and n values represent the magnitude of CPE, unit imaginary number, angular frequency, and a constant that determines the nature of the capacitance, respectively.
  • FIG. 4A illustrates the system for interfacing with rat hearts, where the MEA is attached to the left ventricle of a Langendorff-perfused heart for electrogram (EG) mapping, synchronized with a two complementary metal-oxide-semiconductor (CMOS) cameras-based optical mapping system for concurrent transmembrane potential (Vm) and intracellular calcium (Ca 2+ ) fluorescence mapping.
  • EG Langendorff-perfused heart for electrogram
  • CMOS complementary metal-oxide-semiconductor
  • the high transparency (between 77.6% and 80.3%) of the device from 520 to 780 nm enables efficient passage of the excitation and emission photons from the light source and voltage- and calcium-sensitive dyes (RH237 and Rhod-2 AM), allowing for multiparametric high-content assessment of cardiac tissue electrophysiological function from the same field of view (FIG. 4A bottom).
  • FIG. 4B demonstrates the time-aligned ECG reference (blue line) recorded by Ag/AgCl electrodes, EG signals from all 16 MEA channels (black lines), and simultaneous and co-localized Vm (red lines) and Ca 2+ (purple lines) fluorescence signals from the area beneath each transparent microelectrode during sinus rhythm.
  • the average heart rate recorded by the Mo nanogrid MEA EG results is 176 ⁇ 1.1 beats per minute (BPM), which is consistent with that (176 ⁇ 1.6 BPM) from the far-field ECG.
  • the Mo nanogrid MEA collects cardiac wave propagation profiles from the epicardium, while the voltage fluorescence signals present the dynamic Vm of cardiomyocytes located approximately 0.5-1 mm in depth of the cardiac tissue.
  • the inward calcium channels open during the membrane depolarization allowing an influx of calcium ions into the cell.
  • These calcium ions trigger the opening of the calcium-release channel of the sarcoplasmic reticulum membrane, resulting in the massive release of calcium into the cytosol to activate mechanical contractions.
  • This process of calcium-induced calcium release causes a time delay between membrane depolarization and intracellular calcium transient, which is critically important in synchronizing cardiac contraction.
  • the calculated EG-Ca 2+ activation delay (5.49 ⁇ 0.26 ms) matches well with the optical Vm-Ca 2+ activation delay (5.33 ⁇ 0.87 ms), demonstrating the high-fidelity recording performance of the MEA to investigate cardiac excitation-contraction coupling.
  • FIG. 4C displays the electrical activation map (middle) constructed from the depolarization (activation) times of the MEA recorded EG signals, optical V m (left) and Ca 2+ (right) activation maps. All three maps are synchronized, with white squares highlighting the region covered by the transparent MEA.
  • the total epicardial activation time obtained from the same region via the MEA and optical mapping is 1.6 and 1.5 ms, respectively.
  • the extracted apparent conduction velocity by the MEA is 257.7 cm/s during sinus rhythm.
  • Apparent conductivity characterizes the propagation speed of depolarization waves from the epicardial surface, while the electrical waves originate from the sinoatrial node (e.g., sinus rhythm) and spread out through a complex three-dimensional architecture.
  • the comparison between the EG, Vm, and Ca 2+ activation maps visualizes the spatial-temporal distribution of the EG-Ca 2+ and Vm-Ca 2+ activation delays described previously.
  • the high correlation between the EG and optical Vm maps of the same region further emphasizes the high-fidelity mapping capabilities of the MEA.
  • the above results demonstrate that the Mo nanogrid MEA enables seamless interrogation of three notable cardiac parameters (EG, Vm and Ca 2+ ) via different imaging modalities to accurately detect cardiac wave propagation patterns as well as electrical and mechanical dynamics.
  • the Mo nanogrid MEA is capable of electrical pacing using one or more microelectrodes in the array to modulate cardiac activity, treat abnormalities in the electromechanical properties, and simultaneously assess the pacing effects by electrical mapping using the rest microelectrodes.
  • FIG. 4D demonstrates the EG, Vm, and Ca 2+ activation maps upon electrical stimulation (400 BPM) delivered from the top right comer microelectrode in the MEA.
  • the extracted longitudinal (transverse) conduction velocities from the MEA and optical mapping during pacing are 56.9 (32.4) and 57.6 (32.6) cm/s, respectively.
  • FIG. 4E summarizes representative QRS complexes recorded by the Mo nanogrid MEA across a relevant therapeutic period (Day 0 to Day 3) during sinus rhythm. As the device gradually dissolves in PBS, the amplitude of the recorded QRS complexes becomes smaller. Gradually, the calculated SNR decreases from 35.7 ⁇ 0.74 to 33.9 ⁇ 0.68, 30.2 ⁇ 0.71, and 26.8 ⁇ 0.80 dB after 0, 1, 2, and 3 days in PBS, respectively.
  • FIG. 5A displays the system, where the MEA is placed on the left ventricle of an actively contracting rat heart.
  • FIG. 5B shows the cardiac wave propagation recorded by the MEA during sinus rhythm with an average heart rate of 378 ⁇ 1.9 BPM, which is faster than the ex vivo rat heart excitation in FIG. 4C (177 ⁇ 1.1 BPM) due to autonomic innervation of the cardiac conduction system and various circulating humoral factors and metabolic substrates absent from a Langendorff perfused heart.
  • FIG. 5B shows the cardiac wave propagation recorded by the MEA during sinus rhythm with an average heart rate of 378 ⁇ 1.9 BPM, which is faster than the ex vivo rat heart excitation in FIG. 4C (177 ⁇ 1.1 BPM) due to autonomic innervation of the cardiac conduction system and various circulating humoral factors and metabolic substrates absent from a Langendorff perfused heart.
  • FIG. 5C show that the MEA can concurrently manipulate and map the cardiac wave propagation patterns from an in situ heart by unipolar pacing at 400 BPM (voltage: 2 V, pulse width: 2 ms) at different locations.
  • unipolar pacing In the unipolar pacing mode, one Mo nanogrid microelectrode functions as the cathode, and a remote Pt electrode works as the anode.
  • bipolar pacing is generally preferred because it requires a lower pacing threshold that consumes less energy, and minimizes pectoral muscle stimulation, which may occur during unipolar pacing due to current return to the pulse generator.
  • 5D illustrates the control and capture of the cardiac excitation wave activation and propagation by bipolar pacing using the Mo nanogrid MEA (voltage: 1.5 V, pulse width: 2 ms), where two nearby microelectrodes in the MEA serve as the anode and cathode.
  • Cardiac pacing is frequently used in the clinic to treat abnormal heart rhythms such as bradycardia, where the heartbeat is too slow to support the normal cardiovascular circulation. Bradycardia can be caused by several reasons, such as congenital heart defects, postsurgical complications, TAVR, and AV block.
  • AV block is an interruption in transmitting an impulse between the atria and the ventricles, resulting in the desynchronization of atrial and ventricular excitation and a reduced heart rate.
  • Ventricular pacing is widely used to re-synchronize ventricular and atrial contractions, restore normal heart rate and hemodynamics, and treat patients with AV block.
  • FIG. 5E demonstrates the detection and treatment of AV block with the Mo nanogrid MEA.
  • the ECG signals show a 3: 1 AV block with ventricular contractions occurring at the slow rate of 58 BPM, indicated by three consecutive P waves and only one QRS complex.
  • the ECG traces and EG signals recorded by the 14 sensing microelectrodes exhibit clear high-amplitude and wide QRS complexes during bipolar pacing at 400 BPM, showing the successful rhythm capture of the beating heart.
  • Different shapes of the EG traces during bipolar pacing represent different depolarization and repolarization responses of the local cardiac tissue beneath each microelectrode.
  • the MEA maps different propagation patterns of the depolarization waves during AV block and pacing (FIG. 15).
  • the measured longitudinal and transverse conduction velocities of the depolarization wave during MEA-initiated bipolar pacing are 51.1 and 31.6 cm/s, respectively.
  • the AV block resumes with the rate of ventricular contractions of 55 BPM, indicating that the device pacing is responsible for and required to maintain a normal heart rate.
  • FIG. 5F summarizes the QRS complexes recorded by the Mo nanogrid MEAs on Day 0, 1, 2, 3, 4 during sinus rhythm. The dissolution is completed in PBS while the EG mapping is performed on the blood-perfused beating hearts during in vivo open-chest measurements.
  • FIG. 6A displays the optical images of the hearts in situ (square box) and after explantation (circular box).
  • the optical image at Week 0 shows that the MEA is mechanically robust to survive the surgical handling during implantation.
  • the MEA partially dissolves with visible Mo pattern residues.
  • the MEA completely disappears from the heart surface at the implantation region. Fibrotic tissues and non-bioresorbable prolene sutures exist in all rats of the MEA and sham groups, resulting in tissue damage during surgeries (e.g., stitching the epicardium).
  • FIG. 6B demonstrates the representative histology images of Masson’s trichrome staining of cardiac tissues undergoing MEA implantation surgeries, where the red color represents muscle fiber (i.e., cardiomyocytes) staining, the blue color represents collagen (i.e., fibrotic area) staining, and the white color represents interstitial space.
  • the green triangle indicates the epicardial side. Fibrosis occurs over the epicardial region where the MEAs are implanted.
  • the normal myocardium comprises several major cell types (e.g., cardiomyocytes, fibroblasts, neurons, endothelial cells, etc.), forming a complex three- dimensional architectural network including the collagen scaffold and interstitial space.
  • Pathophysiological conditions e.g., cardiac injury', infarction, heart failure, infections
  • myocardial fibrosis and interstitial space expansion which, in turn, increase ventricular stiffness and lead to decreased electrical synchronization and mechanical contractility.
  • FIG. 6C exhibits the quantification of the histology examination, where the percentage volume of cardiomyocytes, fibrosis, and interstitial space reveals no statistically significant difference among all the groups (P ⁇ 0.05), which indicates negligible effects on the myocardial structures upon the MEA implantation and bioresorption.
  • FIG. 6D depicts the transthoracic echocardiogram test on the contractile function of the heart at different time points after implantation. Stroke volume and ejection fraction represent the volume and percentage of oxygen-rich blood pumped out from the left ventricle during each systolic contraction. A very low ejection fraction indicates impaired pumping action of the heart, which affects normal hemodynamics and is a hallmark of heart failure.
  • FIG. 6E demonstrates the weights of the animals at different time points after implantation. An anticipated weight loss immediately after surgery is followed by a normal, gradual weight gain with age.
  • the serology tests provide a comprehensive understanding of the health status of animals at different stages post-surgery (FIGS. 6F-6J and FIGS. 18A, 18B, 19A-19D).
  • the blood levels of lipids, enzymes, metabolic wastes, immune cells, and electrolytes serve as indicators of organ-specific function and impairment as well as foreign body response upon the MEA implantation and bioresorption. Specifically, high cholesterol and triglycerides are associated with a higher risk of stroke or heart attack.
  • Alkaline phosphatase reflects the function of liver to form and release bile while elevated alanine aminotransferase indicates potential hepatocellular injury.
  • the fabrication of the Mo nanogrid MEA 225 starts at step 1, with laminating an aluminum (Al) foil on a handling glass slide with polyimide tapes (e.g., Advanced Polymer Tape Inc.) on the edges.
  • Oxygen plasma 50 W, 180 mTorr, 3 mins
  • PMMA poly(methyl methacrylate) resist
  • E-beam Electron beam evaporation deposited an 8 nm-thick Cr layer onto the PMMA layer to serve as a conductive layer for EBL.
  • EBL defined the nanogrid MEA structure 225 via a beam current of 1 nA.
  • Sputter deposition prepared a 1000 nm-thick Mo on the MEA followed by a lift-off process in acetone.
  • Photolithography with AZ nLOF 2070 photoresist (Integrated Micro Materials) defined the interconnects 230 and bonding pad structures of the MEA.
  • a second sputter deposition of Mo (1000 nm-thick) followed by liftoff in acetone completed the fabrication of the interconnects 230. [00134] Afterwards, at step 3.
  • a 25 wt% PLGA (65:35, Sigma-Aldrich Inc.)/ethyl acetate (anhydrous, 99.8%, Sigma- Aldrich Inc.) solution is spin coated onto the sample, followed by baking at 60 °C for 5 h and 110 °C for 2 h. Then, at step 4, the film is delaminated from the glass slide by gently peeling off the polyimide tapes on the edges without deforming the film. Finally, at step 5, immersing in hydrochloric acid (37%, High Purity Products) released the transparent Mo nanogrid MEA from the Al foil.
  • the encapsulation PLGA layer 300 is prepared. A 15 wt%
  • PLGA/ethyl acetate solution was spin coated on a soft lithography defined polydimethylsiloxane (PDMS) stamp, followed by baking at 60 °C for 2 h. After cooling down, the PLGA encapsulation layer 300 was gently peeled off from the PDMS stamp. The prepared PLGA encapsulation layer 300 and Mo nanogrid MEA 250 were then aligned together under a microscope, baked at 50 °C for 2 mins, and bonded in ethyl acetate vapor for 3 h. Finally, the device was baked at 50 °C for another 5 mins to improve the adhesion.
  • PDMS polydimethylsiloxane
  • a spectrophotometer (e.g., V-770 UV-vis/NIR, Jasco Inc.) measured the transmission spectra of the Mo nanogrid MEAs.
  • SEM e.g., PIONEER EBL, Raith Inc.
  • a FEI TalosTM F200X scanning transmission electron microscope performed HRTEM and EDS measurements.
  • a motorized test stand e.g., ESM 1500, Mark-10) performed mechanical bending tests, in which the electrochemical performance was measured separately after a specific cycle of bending (FIGS. 20A, 20B).
  • EIS and CV measurements were conducted by a Garnry potentiostat (Reference 600+, Garmy 7 Instruments Inc.) via a three-electrode configuration in l x PBS (FIG. 21).
  • an Ag/AgCl electrode, a Pt electrode, and the Mo nanogrid microelectrode served as the reference, counter, and working electrodes, respectively.
  • CSC c was calculated at a 50 mV/s scan rate.
  • a data acquisition system e.g., PowerLab 16/35, ADInstruments Inc.
  • the microelectrodes in the Mo nanogrid MEAs detected the signals.
  • the recorded signals were processed using MATLAB to obtain SNR and RMS noise.
  • the rat was anesthetized using isoflurane (1-3%). Once sedated, an intraperitoneal injection of buprenorphine (0.5-1.0 mg/kg) analgesia was administered. The rat was then placed supine on the intubation stage, and intubation was performed using the standard technique. Once intubated, the rat was placed on pressure control ventilation in the right lateral decubitus position. Animal ventilation was provided by the VentElite small animal ventilator (Harvard Apparatus, Holliston, MA). ECG leads were connected for intraoperative cardiac monitoring (lead II configuration). The left lateral chest was shaved and prepped using a sterile skin prep.
  • the rat was then covered with a sterile drape, exposing the surgical site.
  • the chest was palpated to identify the intercostal space where the point of maximum impulse could best be palpated.
  • Scissors were used to make a curvilinear incision through the skin and subcutaneous tissue across the chest wall over the intercostal space.
  • Metzenbaum scissors were then used to dissect through the chest wall muscles and into the thoracic cavity with care taken not to injure the lung.
  • a rib spreader then opened the intercostal space.
  • a cotton swab gently retracted the lung to expose the heart. Another cotton swab gently removed the pericardium.
  • the MEA was then laid over the left ventricle.
  • n 3 Sprague-Dawley rats for each group (i.e., control, MEA implantation, sham). Control group did not receive any surgery and echocardiogram was performed directly. Echocardiogram of the MEA implantation group was performed at 2-, 4-, 6-, and 9-weeks post the implantation surgery. Echocardiogram of the sham group was performed at 2-weeks post the sham surgery. Rats were anesthetized by inhalation of 1-3% isoflurane vapors at 2 mL/min oxygen flow before and during the echocardiogram imaging with an EZ anesthesia machine (EZ Systems Inc., EZ-SA 800).
  • Rats were transferred to the imaging stage after confirmation of loss of consciousness. Paws were affixed to the ECG electrodes (of the imaging stage) with electrode gel and tape to monitor the heart rate throughout echocardiogram imaging. Heart rate was maintained at 250-300 BPM. The left lateral chest was shaved, and ultrasound gel (Aquasonics) was applied to the skin. M-mode echocardiography of the left ventricle was performed using the Vevo 3100 system (VisualSonics/Fujifilm). The data were analyzed with VevoLAB2.1.0.
  • n 3 Sprague-Dawley rats for each group (i.e., control, MEA implantation, sham). Control group did not receive any surgery and blood was collected directly. Blood from the MEA implantation group was collected at 2-, 4-, 6-, and 9-weeks post the implantation surgery. Blood from the sham group was collected at 2-weeks post the sham surgery. Blood was stored in (1) serum tubes (Sarstedt Inc.. 1.3 mL micro tubes) for serum complete chemistry tests and (2) K3 EDTA tubes (Sarstedt Inc., 1.3 mL micro tubes) for complete blood count tests. A commercial company (Charles River Laboratories) conducted the assays.
  • n 3 Sprague-Dawley rats for each group (i.e., control. MEA implantation, sham). Control group did not receive any surgery and hearts were collected directly. Hearts from the MEA implantation group were collected at 2-, 4-, 6-, and 9-weeks post implantation surgery'. Hearts from the sham group were collected at 2-weeks post the sham surgery 7 . Rats were euthanized using 5% isoflurane vapors at 2 mL/min oxygen flow with an EZ anesthesia machine (EZ Systems Inc.) until loss of consciousness was confirmed via toe pinch.
  • EZ anesthesia machine EZ Systems Inc.
  • Hearts were excised and retrogradely perfused via an aortic cannula with cardioplegic solution (in mM, NaCl 110, KC1 16, MgCb 16, NaHCCh 10, CaCh 1.2, 4 °C) and then 10% neutral- buffered formalin.
  • Hearts were transferred to a 70% ethanol solution after 24 hours of immersion (room temperature) in 10% neutral -buffered formalin.
  • Cross sections of hearts were paraffin embedded, sectioned, and stained with Masson’s tri chrome for identification of myocardium (red color), fibrosis (blue color), and interstitial space (white color).
  • Tissue samples were imaged using an EVOS XL light microscope (Thermo Fisher Scientific).
  • a custom MATLAB code was used to quantify the percent volume of cardiomyocytes, fibrosis, and interstitial space in the images (i.e., calculate the relative number of pixels per color in the selected region of interest).
  • Optical mapping was performed as previously described. 10-15 pM blebbistatin (Cayman Chemicals, catalog number 13186) was added into the perfusion solution to suppress cardiac contractions.
  • the voltage-sensitive dye RH237 (1.25 mg/mL dye stock solution, Biotium, catalog number 61018)
  • calcium-sensitive dye Rhod-2 AM (1 mg/mL dye stock solution, Thermo Fisher Scientific, catalog number R1244) were added via bolus injection through the cannula. Fluorescence dyes were perfused for 15-20 mins (i.e., equilibration period) before optical mapping studies to allow tissue staining and the washout of extra dyes.
  • Excitation light at 520 ⁇ 17 nm wavelength was used (LEX3-G Green LED System).
  • Vm fluorescence was filtered by a 695 nm long-pass filter and calcium fluorescence was filtered by a 572 ⁇ 14 nm bandpass filter.
  • Images of the front-lateral side of the left ventricle were captured at a speed of 2000 frames per second using two CMOS cameras (ULTIMA-L, SciMedia) with a 100 x 100-pixel resolution (15 x 15 mm 2 field of view).
  • the MEA was placed on the front-lateral side of the left ventricle and connected to a data acquisition system (PowerLab 16/35, ADInstruments Inc.) with a sampling frequency of 20 kHz.
  • Customized Pt bipolar electrodes or the Mo MEA were used to pace the heart from the left ventricle at rates higher than the intrinsic sinus rhythm of the heart.
  • Electrical stimulation amplitude i.e., pacing voltage
  • Tests with donor human ventricular tissue slices were approved by the Institutional Review Board at Northwestern University. Left ventricular slices were generated as previously described using a precision vibrating microtome in an ice-cold slicing solution (in mM, NaCl 140. KC1 6, MgCh 1, CaCh 1.8, glucose 10, HEPES 10, 2,3- butanedionemonoxime 10. pH 7.4 at 4 °C). The system is shown in FIG. 22.
  • Slices were then allowed to recover at room temperature in a recovery solution (in mM, NaCl 140, KC1 4.5, MgCh 1, CaCh 1.8, glucose 10, HEPES 10, pH 7.4 at room temperature) for at least 20 mins before transferring to a bath at 37 °C and superfused with a modified Tyrode’s solution (in mM, NaCl 128.2, NaHCCh 20, NaH 2 PO 4 1.19, KC1 4.7, MgCh 1.05. CaCh 1.3, glucose 11.1, blebbistatin 10-15, pH 7.4 at 37 °C). After a 20 mins equilibration period, slices were stained with RH237 for voltage optical mapping.
  • a recovery solution in mM, NaCl 140, KC1 4.5, MgCh 1, CaCh 1.8, glucose 10, HEPES 10, pH 7.4 at room temperature
  • the MEA w as positioned on the slice in the field of view of the camera. Slices were paced using a Pt bipolar electrode placed adjacent to the device at 1.5 x amplitude of the threshold of stimulation. Optical traces were analyzed using a custom MATLAB software. Rhythm 3.0.
  • Sprague-Dawley rats were anesthetized by 1-3% isoflurane inhalation until there was no response to a toe pinch.
  • the rat was intubated and placed on pressure control ventilation in the right lateral decubitus position.
  • Animal ventilation was provided by the VentElite small animal ventilator (Harvard Apparatus, Holliston, MA).
  • ECG leads were connected for cardiac monitoring (lead II configuration), such as for example via the interconnect 230.
  • the left lateral chest was shaved and palpated to identify the intercostal space where the point of maximum impulse could best be palpated.
  • Scissors were used to make an incision through the skin and dissect the chest wall muscles to expose the thoracic cavity with care taken not to injure the lung.
  • a rib spreader was used to open the intercostal space.
  • a cotton swab was used to gently retract the lung to expose the heart.
  • Another cotton swab was used to gently remove the pericardium.
  • AV block was induced as previously reported. Briefly, animals received (intraperitoneally, IP) 120 mg/kg caffeine (Millipore Sigma, catalog number C0750) and 60 mg/kg dobutamine (Cayman, catalog number 15582) sequentially.
  • Fast pacing (cycle length 100 ms) was applied to the left ventricle for about 15 mins after drug treatment until an AV block was induced.
  • the Mo nanogrid MEA was laid over the heart, covering the front-lateral side of the left ventricle.
  • the MEA was connected to a data acquisition system (PowerLab 16/35, ADInstruments Inc.) with a sampling frequency of 20 kHz, such as for example via the interconnect 230.
  • a data acquisition system PowerLab 16/35, ADInstruments Inc.
  • a sampling frequency such as for example via the interconnect 230.
  • Pt bipolar electrodes or the MEA were used to pace the heart from the left ventricle at rates higher than the intrinsic sinus rhythm of the heart.
  • Electrical stimulation amplitude i.e., pacing voltage
  • Rats were euthanized after tests. Cervical dislocation and heart extraction were performed after confirmation of no response to a toe pinch.
  • bioresorbable While the description indicates that certain materials are bioresorbable, they need not be 100% bioresorbable, but rather dissolve to a sufficient extent to pass safely from the body or otherwise avoid harm to the body so that they- do not need to be manually removed from the body, and still be considered to be ‘‘bioresorbable’' or “absorbable” within the spirit and scope of the present disclosure. And, the substrate layer 100, active layer 200, and encapsulation layer 300 need not be 100% transparent, but only sufficiently transparent to let light or signals pass through the respective layer.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Surgery (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medical Informatics (AREA)
  • Physiology (AREA)
  • Neurosurgery (AREA)
  • Prostheses (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)

Abstract

Des réseaux de microélectrodes transparents (MEA) qui permettent une investigation multimodale des caractéristiques cardiaques spatiotemporelles sont importants dans l'étude et le traitement d'une maladie cardiaque. Cependant, les dispositifs implantables existants sont conçus pour prendre en charge des durées de vie utiles chroniques et exigent une extraction chirurgicale lorsqu'ils présentent un dysfonctionnement ou ne sont plus nécessaires. Par ailleurs, des systèmes biorésorbables qui peuvent s'auto-éliminer après avoir effectué des fonctions provisoires suscitent de plus en plus d'intérêt car ils évitent les coûts/risques d'extraction chirurgicale. L'invention concerne une conception, une fabrication, une caractérisation et une validation d'une plateforme MEA souple, entièrement biorésorbable et transparente pour une interface cardiaque bidirectionnelle sur une période cliniquement pertinente. Le MEA permet un mappage électrique/optique multiparamétrique de dynamique cardiaque et une stimulation sur demande spécifique à un site pour étudier et traiter des dysfonctionnements cardiaques dans des modèles cardiaques de rat et d'humain. La dynamique de biorésorption et la biocompatibilité sont étudiées. Les conceptions de dispositif servent de base à des technologies cardiaques biorésorbables pour la surveillance post-chirurgicale potentielle et le traitement d'états pathologiques temporaires de patient dans certains scénarios cliniques tels que l'infarctus du myocarde, l'ischémie et le remplacement d'une bioprothèse valvulaire aortique par voie transcathéter.
PCT/US2024/036401 2023-06-30 2024-07-01 Réseau de microélectrodes souple, biorésorbable et transparent pour applications cardiaques Ceased WO2025007130A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363524485P 2023-06-30 2023-06-30
US63/524,485 2023-06-30

Publications (2)

Publication Number Publication Date
WO2025007130A2 true WO2025007130A2 (fr) 2025-01-02
WO2025007130A3 WO2025007130A3 (fr) 2025-04-03

Family

ID=93939990

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/036401 Ceased WO2025007130A2 (fr) 2023-06-30 2024-07-01 Réseau de microélectrodes souple, biorésorbable et transparent pour applications cardiaques

Country Status (1)

Country Link
WO (1) WO2025007130A2 (fr)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9936574B2 (en) * 2009-12-16 2018-04-03 The Board Of Trustees Of The University Of Illinois Waterproof stretchable optoelectronics
EP2974673B1 (fr) * 2010-03-17 2017-03-22 The Board of Trustees of the University of Illionis Dispositifs biomédicaux implantables sur substrats biorésorbables
US10292263B2 (en) * 2013-04-12 2019-05-14 The Board Of Trustees Of The University Of Illinois Biodegradable materials for multilayer transient printed circuit boards
EP3052017B1 (fr) * 2013-10-02 2019-12-11 The Board of Trustees of the University of Illionis Dispositif électronique installé sur un organe
US9861288B2 (en) * 2014-07-11 2018-01-09 Wisconsin Alumni Research Foundation Transparent and flexible neural electrode arrays

Also Published As

Publication number Publication date
WO2025007130A3 (fr) 2025-04-03

Similar Documents

Publication Publication Date Title
Chen et al. Soft, bioresorbable, transparent microelectrode arrays for multimodal spatiotemporal mapping and modulation of cardiac physiology
Zhang et al. Millimetre-scale bioresorbable optoelectronic systems for electrotherapy
Choi et al. Adhesive bioelectronics for sutureless epicardial interfacing
Lin et al. Graphene biointerface for cardiac arrhythmia diagnosis and treatment
Sunwoo et al. Stretchable low‐impedance nanocomposite comprised of Ag–Au core–shell nanowires and Pt black for epicardial recording and stimulation
Choi et al. Fully implantable and bioresorbable cardiac pacemakers without leads or batteries
Song et al. Wearable and implantable electronics: moving toward precision therapy
Sunwoo et al. Stretchable low-impedance conductor with Ag–Au–Pt core–shell–shell nanowires and in situ formed Pt nanoparticles for wearable and implantable device
Obaid et al. Multifunctional flexible biointerfaces for simultaneous colocalized optophysiology and electrophysiology
Hwang et al. In situ diagnosis and simultaneous treatment of cardiac diseases using a single-device platform
Kim et al. Three-dimensional electrodes of liquid metals for long-term, wireless cardiac analysis and modulation
Li et al. Long-term in vivo operation of implanted cardiac nanogenerators in swine
Han et al. Next-generation cardiac interfacing technologies using nanomaterial-based soft bioelectronics
Wu et al. Orbit symmetry breaking in MXene implements enhanced soft bioelectronic implants
Xue et al. Flexible multichannel vagus nerve electrode for stimulation and recording for heart failure treatment
Tang et al. Soft bioelectronics for cardiac interfaces
US11419535B2 (en) Nanomesh electrode structures and techniques for the formation thereof
Zhao et al. Robust, stretchable bioelectronic interfaces for cardiac pacing enabled by interfacial transfer of laser-induced graphene via water-response, nonswellable PVA gels
Liu et al. Carbon-based implantable bioelectronics
Lee et al. Engineering implantable bioelectronics for electrophysiological monitoring in preclinical animal models
KR20160056198A (ko) 심장의 재동기화 치료를 위한 그물망 전극 및 이의 제조 방법
Obaid et al. Flexible electro‐optical arrays for simultaneous multi‐site colocalized spatiotemporal cardiac mapping and modulation
Xiang et al. High-density stretchable microelectrode array based on multilayer serpentine interconnections
Yang et al. Novel electroactive therapeutic platforms for cardiac arrhythmia management
Kasavetov et al. PEDOT: PSS conducting eutectogel for enhanced electrical recording and stimulation in implantable neural interfaces

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24833148

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 24833148

Country of ref document: EP

Kind code of ref document: A2