WO2025009748A1 - Composition antioxydante et anti-inflammatoire comprenant un extrait de feuille de carotte à l'eau de mer volcanique en tant que principe actif - Google Patents

Composition antioxydante et anti-inflammatoire comprenant un extrait de feuille de carotte à l'eau de mer volcanique en tant que principe actif Download PDF

Info

Publication number
WO2025009748A1
WO2025009748A1 PCT/KR2024/007198 KR2024007198W WO2025009748A1 WO 2025009748 A1 WO2025009748 A1 WO 2025009748A1 KR 2024007198 W KR2024007198 W KR 2024007198W WO 2025009748 A1 WO2025009748 A1 WO 2025009748A1
Authority
WO
WIPO (PCT)
Prior art keywords
lava seawater
extract
carrot
inflammatory
carrot leaf
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2024/007198
Other languages
English (en)
Korean (ko)
Inventor
김소미
양선희
윤태현
이태백
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Industry Academic Cooperation Foundation of Jeju National University
Original Assignee
Industry Academic Cooperation Foundation of Jeju National University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Industry Academic Cooperation Foundation of Jeju National University filed Critical Industry Academic Cooperation Foundation of Jeju National University
Publication of WO2025009748A1 publication Critical patent/WO2025009748A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/02—Medicinal preparations containing materials or reaction products thereof with undetermined constitution from inanimate materials
    • A61K35/08—Mineral waters; Sea water
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/965—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of inanimate origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying

Definitions

  • the present invention relates to an antioxidant and anti-inflammatory composition containing carrot leaf lava seawater extract as an effective ingredient.
  • Carrots are not only a good source of minerals such as calcium, phosphorus, iron, and magnesium, but also one of the root vegetables rich in various physiologically active compounds such as carotenoids and dietary fiber. Due to the various nutritional benefits of carrots, the consumption of carrots has been steadily increasing worldwide. However, the aerial parts of carrots are not yet widely used for food. Therefore, if high value-added products are developed through functional research on the aerial parts of carrots, they can be used as a new source of income for farmers.
  • lava seawater is groundwater that has been naturally filtered by volcanic bedrock.
  • Jeju Island it has been used as breeding water for flounder farms since the 1980s, taking advantage of its low temperature and cleanliness characteristics. Its industrial utility value in beauty, food, spas, etc. is highly evaluated and is receiving much attention.
  • the lava seawater distributed in the eastern part of Jeju Island (Gujwa-eup, Seongsan-eup, and Pyoseon-myeon) is clean and has a constant water temperature throughout the year. It also contains a large amount of beneficial minerals, including essential minerals such as sodium, calcium, and magnesium, as well as rare minerals such as vanadium, germanium, and selenium that are not found in general groundwater and seawater. Accordingly, research on the stability and various physiological activities of the lava seawater itself has been actively conducted, but there has been little research on the production of extracts using the lava seawater.
  • Lava seawater has similar mineral components to deep seawater, but it has the advantage of being economical as it is cheaper to obtain than deep seawater. Therefore, it is possible to develop high value-added products through various functional studies of extracts using lava seawater.
  • Korean Patent Publication No. 2023-0081972 discloses a hair loss prevention/hair growth promotion composition containing lava seawater and natural extracts and a method for producing the same
  • Korean Registration Patent No. 2104861 discloses a method for producing lava seawater-aged Zelkova leaf powder and a composition for improving skin wrinkles using an extract of the powder.
  • an antioxidant and anti-inflammatory composition containing the carrot leaf lava seawater extract of the present invention as an effective ingredient.
  • the present invention was derived from the above-mentioned needs, and provides an antioxidant and anti-inflammatory composition containing a carrot leaf lava seawater extract as an effective ingredient, and completes the present invention by confirming that the total polyphenol, rutin and caffeic acid contents of the carrot leaf lava seawater extract of the present invention are increased and the antioxidant and anti-inflammatory activities are enhanced compared to the carrot leaf water extract.
  • the present invention provides an antioxidant and anti-inflammatory cosmetic composition containing carrot leaf lava seawater extract as an effective ingredient.
  • the present invention provides an antioxidant and anti-inflammatory health functional food composition containing carrot leaf lava seawater extract as an effective ingredient.
  • the present invention provides an anti-inflammatory pharmaceutical composition containing carrot leaf lava seawater extract as an effective ingredient.
  • the present invention provides an antioxidant and anti-inflammatory feed additive containing carrot leaf lava seawater extract as an effective ingredient.
  • the present invention provides a method for producing a carrot extract having increased contents of rutin, caffeic acid and total polyphenols and enhanced antioxidant and anti-inflammatory effects, the method comprising the step of adding water containing 10 to 20% (v/v) of demineralized lava seawater to pulverized carrot leaves and extracting the same.
  • the present invention relates to an antioxidant and anti-inflammatory composition containing a carrot leaf lava seawater extract as an effective ingredient.
  • the carrot leaf lava seawater extract of the present invention has increased total polyphenol, rutin, and caffeic acid contents, has excellent DPPH radical scavenging activity, and has a remarkable effect of inhibiting NO production compared to a carrot leaf water extract. Therefore, the carrot leaf lava seawater extract of the present invention can be usefully used as an antioxidant and anti-inflammatory composition.
  • Figure 1 shows the results of confirming the total polyphenol content of carrot leaf extracts according to the content of desalinated lava seawater in the solvent. **** means that the total polyphenol content of 15% (v/v) and 30% (v/v) desalinated lava seawater extracts of carrot leaves increased statistically significantly compared to the carrot leaf water extract, and p ⁇ 0.001.
  • Figure 2 shows the results of confirming the DPPH radical scavenging activity of carrot leaf extracts according to the content of demineralized lava seawater in the solvent. **** means that the DPPH radical scavenging activity of 15% (v/v) and 30% (v/v) demineralized lava seawater extracts of carrot leaves statistically significantly increased compared to the carrot leaf water extract, and p ⁇ 0.001.
  • Figure 3 shows the results of confirming the NO production ability of carrot leaf extracts according to the content of desalinated lava seawater in the solvent.
  • LPS is a substance that induces NO production. * means that the NO production inhibition ability of 15% (v/v) and 30% (v/v) desalinated lava seawater extracts of carrot leaves increased statistically significantly compared to the carrot leaf water extract, and p ⁇ 0.05.
  • Figure 4 shows the results of confirming the rutin and caffeic acid contents of carrot leaf extracts according to the content of desalinated lava seawater in the solvent. ***, **** mean that the rutin or caffeic acid content of the 15% (v/v) desalinated lava seawater extract of carrot leaves statistically significantly increased compared to the carrot leaf water extract, and *** means p ⁇ 0.005, and **** means p ⁇ 0.001.
  • the present invention provides an antioxidant and anti-inflammatory cosmetic composition containing carrot leaf lava seawater extract as an effective ingredient.
  • the above carrot leaf lava seawater extract may be extracted using water containing 10 to 20% (v/v) of desalinated lava seawater as a solvent, preferably using water containing 13 to 17% (v/v) of desalinated lava seawater as a solvent, and more preferably using water containing 15% (v/v) of desalinated lava seawater as a solvent, but is not limited thereto.
  • the lava seawater of the present invention may be a product obtained by desalinating water extracted from a depth of 30 to 150 m (based on average sea level) by excavating 100 to 200 m underground in the eastern region of Jeju Island, preferably a product obtained by desalinating water extracted from a depth of 100 to 150 m (based on average sea level) by excavating 100 to 200 m underground in Handong-ri, Gujwa-eup, Bukjeju-gun, eastern region of Jeju Island, more preferably a product obtained by desalinating water extracted from a depth of 130 m (based on average sea level) by excavating 100 to 200 m underground in Handong-ri, Gujwa-eup, Bukjeju-gun, eastern region of Jeju Island, but is not limited thereto.
  • the lava seawater of the present invention is preferably desalinated lava seawater obtained through one or more processes selected from the group consisting of a flash evaporation method, a seawater freezing method, a reverse osmosis method, an ion exchange resin method, and an electrodialysis method, and more preferably, it is obtained through an electrodialysis process in which ions are separated using an ion exchange membrane and an electric current as a driving force from lava seawater collected from 130 m underground in the eastern part of Jeju Island, but is not limited thereto.
  • the desalinated lava seawater of the present invention has a hardness of 5,000 mg/L or more, preferably a hardness of 5,000 mg/L or more and 20,000 mg/L or less, and more preferably a hardness of 5,000 mg/L or more and 6,000 mg/L or less, but is not limited thereto.
  • the above carrot leaf lava seawater extract has the characteristic of increased content of rutin and caffeic acid and total polyphenol content compared to carrot leaf water extract.
  • the cosmetic composition may be prepared in any one formulation selected from a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion, a foundation, a wax foundation, and a spray, but is not limited thereto.
  • a cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these ingredients can be easily selected by a person skilled in the art.
  • the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component.
  • the cosmetic composition of the present invention is in the form of a powder or spray
  • lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane-butane or dimethyl ether may be additionally included.
  • a solvent, a solvating agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.
  • liquid diluents such as water, ethanol or propylene glycol
  • suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. can be used as carrier components.
  • the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkyl amidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used as a carrier component.
  • the cosmetic composition of the present invention may additionally contain excipients including fluorescent substances, fungicides, hydrotropism inducers, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like.
  • excipients including fluorescent substances, fungicides, hydrotropism inducers, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like.
  • the present invention provides an antioxidant and anti-inflammatory health functional food composition containing carrot leaf lava seawater extract as an effective ingredient.
  • the above health functional food composition may be manufactured in any one formulation selected from powder, granules, pills, tablets, capsules, candy, syrup, and beverage, but is not limited thereto.
  • the health functional food composition of the present invention When used as a food additive, the health functional food composition can be added as it is or used together with other foods or food ingredients, and can be used appropriately according to a conventional method.
  • the amount of the effective ingredient can be used appropriately depending on its intended use (prevention or improvement).
  • the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material.
  • the amount can be below the above range, and since there is no problem in terms of safety, the effective ingredient can be used in an amount greater than the above range.
  • Examples of foods to which the above health functional food composition can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea drinks, alcoholic beverages, and vitamin complexes, and include all health foods in the conventional sense.
  • the health functional food composition of the present invention can be manufactured as a food, particularly a functional food.
  • the functional food of the present invention includes ingredients that are usually added during food manufacturing, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings.
  • ingredients that are usually added during food manufacturing, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings.
  • it may include natural carbohydrates or flavoring agents as additional ingredients in addition to the effective ingredient.
  • the natural carbohydrate is preferably a monosaccharide (e.g., glucose, fructose, etc.), a disaccharide (e.g., maltose, sucrose, etc.), an oligosaccharide, a polysaccharide (e.g., dextrin, cyclodextrin, etc.), or a sugar alcohol (e.g., xylitol, sorbitol, erythritol, etc.).
  • the flavoring agent can use a natural flavoring agent (e.g., thaumatin, stevia extract, etc.) and a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).
  • various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. may be further contained.
  • the ratio of these added components is not particularly important, but is generally selected in the range of 0.01 to 0.1 parts by weight with respect to 100 parts by weight of the health functional food composition of the present invention.
  • the present invention provides an anti-inflammatory pharmaceutical composition containing carrot leaf lava seawater extract as an effective ingredient.
  • the pharmaceutical composition containing the effective ingredient of the present invention may further contain an appropriate carrier, excipient or diluent commonly used in the manufacture of pharmaceutical compositions.
  • the pharmaceutical dosage form of the pharmaceutical composition of the present invention can be used alone or in combination with other pharmaceutically active compounds as well as in appropriate combinations.
  • the pharmaceutical composition according to the present invention can be formulated and used in the form of oral preparations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and injections, respectively, according to conventional methods.
  • Carriers, excipients, and diluents that can be included in the pharmaceutical composition include various compounds or mixtures including lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.
  • Solid preparations for oral administration include tablets, pills, powders, granules, and capsules, and these solid preparations are prepared by mixing the above active ingredient with at least one excipient, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc.
  • excipients such as starch, calcium carbonate, sucrose or lactose, gelatin, etc.
  • lubricants such as magnesium stearate and talc are also used.
  • Liquid preparations for oral administration include suspensions, oral solutions, emulsions, and syrups, and in addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, flavoring agents, and preservatives may be included.
  • Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, and suppositories.
  • Non-aqueous solvents and suspending agents can be used, such as propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate.
  • Suppository bases can be used, such as withepsol, macrogol, Tween 61, cocoa butter, laurin butter, and glycerogelatin.
  • the preferred dosage of the pharmaceutical composition of the present invention varies depending on the patient's condition and weight, the degree of the disease, the drug form, the route of administration, and the period of administration, but can be appropriately selected by those skilled in the art.
  • the pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans by various routes. All modes of administration can be expected, and for example, it can be administered by oral, rectal, or intravenous, intramuscular, subcutaneous, intrauterine, epidural, or intracerebroventricular injection.
  • the present invention provides an antioxidant and anti-inflammatory feed additive containing carrot leaf lava seawater extract as an effective ingredient.
  • the feed additive of the present invention corresponds to supplementary feed under the Feed Management Act.
  • the term 'feed' in the present invention may mean any natural or artificial diet, meal, etc., or a component of the meal, which is suitable for animals to eat, ingest, and digest.
  • the type of the feed is not particularly limited, and feed commonly used in the relevant technical field may be used.
  • Non-limiting examples of the feed include plant feeds such as grains, roots, food processing by-products, algae, fibers, pharmaceutical by-products, fats, starches, meal, or grain by-products; animal feeds such as proteins, inorganic substances, fats, minerals, fats, single-cell proteins, zooplankton, or food. These may be used alone or in combination of two or more.
  • the present invention provides a method for producing a carrot extract having increased contents of rutin, caffeic acid and total polyphenols and enhanced antioxidant and anti-inflammatory effects, the method comprising the step of adding water containing 10 to 20% (v/v) of demineralized lava seawater to pulverized carrot leaves and extracting the same.
  • the above extraction may be high-temperature and pressure extraction at 110 to 130°C for 5 to 15 minutes, preferably high-temperature and pressure extraction at 120 to 125°C for 8 to 12 minutes, and more preferably high-temperature and pressure extraction at 121°C for 10 minutes, but is not limited thereto.
  • the carrot leaf powder of the present invention is preferably a powdered product of freeze-dried carrot leaves, but is not limited thereto.
  • the method for producing a carrot extract with increased content of the above routine and enhanced antioxidant and anti-inflammatory effects may include, but is not limited to, adding a step of filtering through a filter paper, concentrating under reduced pressure, and then freeze-drying after the above step.
  • Carrot leaves were collected from Gujwa-eup, Jeju Island, freeze-dried, and then used.
  • the desalinated lava seawater of the present invention was provided and used from the Jeju Lava Seawater Center. Specifically, the desalinated lava seawater of the present invention was produced by using the principle of separating ions that are ionized in lava seawater extracted from 130 m underground in the eastern part of Jeju Island using an ion exchange membrane with an electric current as a driving force, and the desalinated lava seawater of the present invention obtained through the electrodialysis process is a mineral water rich in calcium and magnesium and has the characteristic of a hardness of 5000 mg/L or higher.
  • the solvent used was water not containing demineralized lava seawater (0%), water containing 15% (v/v) of demineralized lava seawater, or water containing 30% (v/v) of demineralized lava seawater.
  • the total polyphenol content of the carrot leaf lava seawater extract manufactured in Manufacturing Example 1 was analyzed.
  • the total polyphenol content was determined by adding 125 ⁇ l of sample to 1,375 ⁇ l of distilled water, adding 0.5 mL of Folin-Ciocalteu's reagent, adding 1 mL of 10% (w/v) Na2CO3 after 3 minutes, and leaving to stand for 30 minutes in the dark at room temperature.
  • the absorbance was measured at 700 nm using a spectrophotometer (Sunrise, Tecan, Salzburg, Austria).
  • Gallic acid was used as a standard, and after creating a standard calibration curve, the total polyphenol content of the sample was expressed as gallic acid equivalents (mg GAE/g).
  • Radical scavenging activity (%) (absorbance of control group - absorbance of extract treatment group) / absorbance of control group ⁇ 100
  • the DPPH radical scavenging activity of the carrot leaf extract treatment group increased in a concentration-dependent manner, and the DPPH radical scavenging activity of the carrot leaf lava seawater extract treatment group significantly increased at all concentrations compared to the carrot leaf water extract treatment group.
  • the DPPH radical scavenging activity was the best.
  • the EC 50 value of the carrot leaf lava seawater extract treatment group decreased compared to the carrot leaf water extract treatment group, and in particular, the EC 50 value of the carrot leaf 15% (v/v) desalinated lava seawater extract treatment group was the lowest.
  • the anti-inflammatory effect of the carrot leaf extract of Manufacturing Example 1 was evaluated by analyzing the inhibition ability of nitric oxide (NO) production in Raw 264.7 cells activated with lipopolysaccharide (LPS).
  • NO nitric oxide
  • LPS lipopolysaccharide
  • the mouse macrophage cell line Raw 264.7 was purchased from the Korean Cell Line Bank and cultured in a medium containing 10% (v/v) FBS, 10,000 units/mL penicillin, 10,000 ⁇ g/mL streptomycin, and 25 ⁇ g/mL amphotericin in DMEM (Gibco, USA) in a completely humidified cell incubator at 37°C and 5% carbon dioxide. All cells were tested at 80–90% confluence, and only cells that had not passed 20 passages were used.
  • the NO production inhibition ability was measured using the Griess analysis method after culturing Raw 264.7 cells (control group) that were not treated with the extract and Raw 264.7 cells (experimental group) that were pretreated with 800 ⁇ g/mL of the sample of Manufacturing Example 1 for 2 hours and stimulated with 1 ⁇ g/mL of LPS for 24 hours.
  • the NO production in the carrot leaf lava seawater extract treatment group decreased compared to the carrot leaf water extract treatment group, and in particular, the NO production reduction effect of the carrot leaf 15% (v/v) desalinated lava seawater extract treatment group was the best.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Birds (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Physiology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medical Informatics (AREA)
  • Pain & Pain Management (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Rheumatology (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

La présente invention concerne une composition antioxydante et anti-inflammatoire comprenant un extrait de feuille de carotte à l'eau de mer volcanique en tant que principe actif. L'extrait de feuille de carotte à l'eau de mer volcanique selon la présente invention a une teneur totale en polyphénol, rutine et acide caféique accrue par rapport à un extrait aqueux de feuille de carotte, a une excellente activité de piégeage de radical de DPPH, et a un effet remarquable d'inhibition de la production de NO. Par conséquent, l'extrait de feuille de carotte à l'eau de mer volcanique selon la présente invention peut être utile en tant que composition antioxydante et anti-inflammatoire.
PCT/KR2024/007198 2023-07-03 2024-05-28 Composition antioxydante et anti-inflammatoire comprenant un extrait de feuille de carotte à l'eau de mer volcanique en tant que principe actif Ceased WO2025009748A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020230085839A KR102847602B1 (ko) 2023-07-03 2023-07-03 당근잎 용암해수 추출물을 유효성분으로 함유하는 항산화 및 항염증용 조성물
KR10-2023-0085839 2023-07-03

Publications (1)

Publication Number Publication Date
WO2025009748A1 true WO2025009748A1 (fr) 2025-01-09

Family

ID=94172340

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2024/007198 Ceased WO2025009748A1 (fr) 2023-07-03 2024-05-28 Composition antioxydante et anti-inflammatoire comprenant un extrait de feuille de carotte à l'eau de mer volcanique en tant que principe actif

Country Status (2)

Country Link
KR (1) KR102847602B1 (fr)
WO (1) WO2025009748A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102885093B1 (ko) * 2025-01-13 2025-11-12 재단법인 제주테크노파크 비건용 식물성 육수의 제조 방법

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060099192A (ko) * 2005-03-10 2006-09-19 (주)바이오뉴트리젠 항산화물질을 함유하는 식물체분말 또는 추출물을 포함하는가축사료첨가제조성물
KR20170001201A (ko) * 2015-06-26 2017-01-04 김선희 당근 잎 추출물을 함유하는 화장료 조성물
KR20190128666A (ko) * 2017-03-01 2019-11-18 더 부츠 캄파니 피엘씨 미용 스킨케어 조성물들의 키트
KR20210072354A (ko) * 2019-12-09 2021-06-17 주식회사 아미코스메틱 추출 수율 및 미네랄 함량이 증진된 화장료 조성물 제조방법 및 이의 제조방법을 이용한 화장료 조성물
KR20220125943A (ko) * 2021-03-08 2022-09-15 오석진 당근잎 분말 및 브로콜리 분말을 함유하는 혈액순환 개선, 항염증, 항비만 및 면역기능 강화용 조성물

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060099192A (ko) * 2005-03-10 2006-09-19 (주)바이오뉴트리젠 항산화물질을 함유하는 식물체분말 또는 추출물을 포함하는가축사료첨가제조성물
KR20170001201A (ko) * 2015-06-26 2017-01-04 김선희 당근 잎 추출물을 함유하는 화장료 조성물
KR20190128666A (ko) * 2017-03-01 2019-11-18 더 부츠 캄파니 피엘씨 미용 스킨케어 조성물들의 키트
KR20210072354A (ko) * 2019-12-09 2021-06-17 주식회사 아미코스메틱 추출 수율 및 미네랄 함량이 증진된 화장료 조성물 제조방법 및 이의 제조방법을 이용한 화장료 조성물
KR20220125943A (ko) * 2021-03-08 2022-09-15 오석진 당근잎 분말 및 브로콜리 분말을 함유하는 혈액순환 개선, 항염증, 항비만 및 면역기능 강화용 조성물

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YANG, S. H. et al. effect of jeju lava seawater on antioxidant and anti-inflammatory activities of daucus carota L. leaves extracts. KSABC international symposium 2023. 18 Jun 2023. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102885093B1 (ko) * 2025-01-13 2025-11-12 재단법인 제주테크노파크 비건용 식물성 육수의 제조 방법

Also Published As

Publication number Publication date
KR102847602B1 (ko) 2025-08-19
KR20250006389A (ko) 2025-01-13

Similar Documents

Publication Publication Date Title
KR102026407B1 (ko) 갈색거저리 발효 추출물을 유효성분으로 포함하는 항산화용 조성물
KR102132655B1 (ko) 구절초 추출물을 포함하는 조성물
WO2020235917A1 (fr) Composition pour soulager ou traiter l'acné, comprenant, en tant que principe actif, un extrait de salvia plebeia r. br. ou une fraction de celui-ci
KR20190041130A (ko) 참당귀 발효 추출물을 유효성분으로 포함하는 면역증진용 조성물
WO2025009748A1 (fr) Composition antioxydante et anti-inflammatoire comprenant un extrait de feuille de carotte à l'eau de mer volcanique en tant que principe actif
KR101125224B1 (ko) 센티페드그라스 추출물 또는 이의 분획물을 유효성분으로 함유하는 피부 노화방지용 조성물
US20240216407A1 (en) Novel polyphenol compound
WO2026071862A1 (fr) Composition pour atténuer l'odeur corporelle gériatrique à l'aide d'un extrait de lysimachia davurica
KR20080109243A (ko) 항당뇨 활성을 나타내는 패과식물의 추출물
KR20170077980A (ko) 큰도꼬마리 어린순 추출물을 함유하는 항산화 또는 항노화용 조성물
KR101692889B1 (ko) 두메닥나무 추출물 또는 이의 분획물을 포함하는 염증성 질환의 예방 또는 치료용 조성물
KR102754013B1 (ko) 멸가치 추출물 또는 네오클로로겐산을 유효성분으로 포함하는 미세먼지에 의한 피부 손상 예방 또는 개선용 조성물
KR101685829B1 (ko) 항산화 활성이 증진된 겨우살이 추출 발효물의 제조방법
KR102076808B1 (ko) 갈조류 추출물을 유효성분으로 포함하는 항산화 또는 항염증 조성물
KR20180013666A (ko) 울다비오시드 a 화합물을 함유하는 항산화 조성물
KR101971986B1 (ko) 참나무겨우살이 추출물을 유효성분으로 포함하는 항암용 조성물
KR102849419B1 (ko) 감귤 신품종 옐로우볼의 과피로부터 분리된 신규 화합물을 유효성분으로 함유하는 항산화 및 항염증용 조성물
KR102847780B1 (ko) 멜로시라 눔물로이드 추출물을 유효성분으로 함유하는 항산화 및 항염증용 조성물
KR100912290B1 (ko) 신규6,8-디(γ,γ-디메틸알릴)-3,5,7,2',4',6'-헥사히드록시플라바논 또는 이의 약학적으로 허용가능한 염, 이의제조방법 및 이를 유효성분으로 함유하는 숙취 해소용조성물
KR100489520B1 (ko) 누리장나무 잎 추출물로부터아피게닌-7-오-베타-디-글루쿠로니드를 분리하는 방법 및이 화합물을 함유하는 위염 및 역류성 식도염 질환 예방및 치료를 위한 조성물
KR102433124B1 (ko) 아세틸쿠마로일슈크로스 유도체 및 이의 용도
KR102274808B1 (ko) 꾸지뽕나무 열매에서 분리된 화합물을 유효성분으로 포함하는 한국형 헬리코박터 파일로리 제균용 조성물
KR101804580B1 (ko) 1,3-디올레인 또는 1,3-디리노레오일-락-글리세롤을 함유하는 피부 과색소성 질환의 개선, 예방 또는 치료용 및 미백용 조성물
KR20200089527A (ko) 석창포 및 황칠 혼합 추출물을 포함하는 항염증용 조성물
KR20200069616A (ko) 올벚나무를 포함하는 항염증용 조성물

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24836197

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE