WO2025123647A1 - Bacillus toyonensis ayant pour effet de prévenir et de lutter contre les pucerons cendrés du chou - Google Patents

Bacillus toyonensis ayant pour effet de prévenir et de lutter contre les pucerons cendrés du chou Download PDF

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Publication number
WO2025123647A1
WO2025123647A1 PCT/CN2024/101846 CN2024101846W WO2025123647A1 WO 2025123647 A1 WO2025123647 A1 WO 2025123647A1 CN 2024101846 W CN2024101846 W CN 2024101846W WO 2025123647 A1 WO2025123647 A1 WO 2025123647A1
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WIPO (PCT)
Prior art keywords
aphids
hxbw
bacillus
preventing
strain
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Pending
Application number
PCT/CN2024/101846
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English (en)
Chinese (zh)
Inventor
倪海平
宗睿
刘金
刘鲁民
何月秋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Hexie Biotechnology Co Ltd
Shandong Baiwo Biotechnology Co Ltd
Original Assignee
Qingdao Hexie Biotechnology Co Ltd
Shandong Baiwo Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Hexie Biotechnology Co Ltd, Shandong Baiwo Biotechnology Co Ltd filed Critical Qingdao Hexie Biotechnology Co Ltd
Publication of WO2025123647A1 publication Critical patent/WO2025123647A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protection of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Definitions

  • Aphids are a general term for the superfamily Aphidoidea of the order Hemiptera, including all members of the superfamily Aphidoidea.
  • the present invention also provides a use of the Bacillus toyota HXBW-2 strain, which is the use in preparing products for preventing and controlling plant pests.
  • the plant pests are caused by cabbage aphids.
  • the present invention also provides a product for alleviating plant insect pests, wherein the product contains live bacteria and/or fermentation products of the HXBW-2 strain of Bacillus sp.
  • the product is a bacterial liquid product.
  • Figure 1 Colony morphology of strains
  • Figure 5 Aphid situation 3 days after using microbial agents, where A is before use and B is the result 3 days after use.
  • the present invention extracted 76 pure bacterial colonies from the rhizosphere soil of plants that often suffer from aphid diseases, one of which had a strong effect of killing cabbage aphids.
  • the strain was identified as Bacillus toyonensis by 16SrRNA sequencing.
  • the fungus has a good control effect on cabbage aphids and can be widely used in the control of cabbage aphids.
  • Mortality (%) number of dead aphids/total number of aphids ⁇ 100.
  • Corrected mortality rate (%) (% mortality rate of the treatment group - % mortality rate of the control group) / (100 - The mortality rate of the control group (%) ⁇ 100
  • NA aphidicide activity
  • a total of 76 strains were isolated from the rhizosphere soil, of which HXBW-2, HXBW-05, HXBW-27, HXBW-31 and HXBW-53, 5 strains had aphidicide activity.
  • the one with strong aphidicide activity was HXBW-2, whose fermentation liquid had a 96.3% aphid mortality rate at 2-fold dilution concentration.
  • HXBW-27 had moderate aphidicide activity, with a 56.7% aphid mortality rate.
  • HXBW-05, HXBW-31 and HXBW-53 showed weak aphidicide activity.
  • Table 1 Toxicity of bacterial fermentation broth to cabbage aphids
  • the pure cultured HXBW-2 strain was streaked on a NA medium plate and cultured in a 25°C incubator for 48 hours. After obvious colonies grew, the colony morphology, color, transparency, dryness, surface smoothness and edge morphology were observed. Fresh bacteria were taken for Gram staining and microscopic examination.
  • the strain is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, with the deposit number CGMCC No. 28561 and the deposit date September 26, 2023; the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.
  • Cabbage aphids were divided into 7 groups, namely experimental groups (high dose group 2 ⁇ 10 9 cfu/mL, medium-high dose group 1 ⁇ 10 9 cfu /mL, medium dose group 1 ⁇ 10 8 cfu/mL, medium-low dose group 4 ⁇ 10 7 cfu/mL, low dose group 2 ⁇ 10 7 cfu/mL), control group (bio group 2 ⁇ 10 9 cfu/mL) and blank control group (ddH 2 O).
  • experimental groups high dose group 2 ⁇ 10 9 cfu/mL, medium-high dose group 1 ⁇ 10 9 cfu /mL, medium dose group 1 ⁇ 10 8 cfu/mL, medium-low dose group 4 ⁇ 10 7 cfu/mL, low dose group 2 ⁇ 10 7 cfu/mL
  • control group bio group 2 ⁇ 10 9 cfu/mL
  • blank control group ddH 2 O
  • the HXBW-2 strain fermentation liquid was diluted 10 times, 20 times, 50 times, and 100 times, the original bacterial liquid and the diluted liquid were used for indoor quantitative spraying insecticidal test, and 1 ⁇ of Tween 20 was added as a dispersant.
  • a certain number of surviving aphids were placed on the leaves of indoor culture dishes to keep them moist, with 100 aphids per dish.
  • the fermentation liquid of the strains at different concentrations was evenly sprayed on the cabbage aphids until the surface of the insect body was moist.
  • the finished product of Bacillus thuringiensis was used as the control group, and water was used as the blank control group.
  • the test was repeated 3 times. After spraying, the culture dishes were sealed with plastic wrap, and a dozen small holes were poked with a needle to ensure air circulation. The aphid mortality rates at 2h, 8h, 24h, and 48h were recorded.
  • Mortality rate (%) number of dead insects (heads) ⁇ 100 / total number of insects treated (heads)
  • the 48h mortality rate was higher than 75%. The lower the concentration, the lower the mortality rate.
  • the mortality rate of the finished product control group (bio group) at 2h, 8h, 24h, and 48h was lower than that of the low-dose group of the experimental group, and the mortality rate after 48h was only 51%.
  • the HXBW-2 strain was tested for its outdoor efficacy against cabbage aphids.
  • the fermentation liquid was diluted to a concentration of 1 ⁇ 10 8 cfu/mL for outdoor field efficacy testing of aphids.
  • the insect population change was detected for 15 days and the efficacy was calculated.
  • the number of cabbage aphids on the roses was adjusted to be as close as possible.
  • the 24 roses were randomly divided into 3 groups with equal numbers and different plots. Leaves of the same size and age were selected from each pot of seedlings as the test objects, randomly sorted 1 to 24, and marked with labels. The number of aphids on each branch was counted and then treated. The number of aphids was observed and recorded regularly, and the control effect was calculated as follows:
  • Insect population reduction rate (%) (number of live insects before application - number of live insects after application) / number of live insects before application ⁇ 100%

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Pest Control & Pesticides (AREA)
  • Forests & Forestry (AREA)
  • Ecology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dentistry (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Agronomy & Crop Science (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Insects & Arthropods (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un type de Bacillus toyonensis a pour effet de prévenir et de lutter contre les pucerons cendrés du chou, qui a un numéro de conservation CGMCC No 28561. La présente invention peut atténuer les dommages des organismes nuisibles provoqués par les pucerons cendrés du chou, et présente un bon effet insecticide à la fois en intérieur et en extérieur.
PCT/CN2024/101846 2023-12-13 2024-06-27 Bacillus toyonensis ayant pour effet de prévenir et de lutter contre les pucerons cendrés du chou Pending WO2025123647A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202311707174.7A CN117683678B (zh) 2023-12-13 2023-12-13 一种具有防治甘蓝蚜虫效果的东洋芽孢杆菌
CN202311707174.7 2023-12-13

Publications (1)

Publication Number Publication Date
WO2025123647A1 true WO2025123647A1 (fr) 2025-06-19

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PCT/CN2024/101846 Pending WO2025123647A1 (fr) 2023-12-13 2024-06-27 Bacillus toyonensis ayant pour effet de prévenir et de lutter contre les pucerons cendrés du chou

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WO (1) WO2025123647A1 (fr)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117683678B (zh) * 2023-12-13 2025-01-24 青岛和协生物科技有限公司 一种具有防治甘蓝蚜虫效果的东洋芽孢杆菌

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106854631A (zh) * 2016-12-26 2017-06-16 青岛蔚蓝生物集团有限公司 一种植物病害的微生物防治方法
CN107205404A (zh) * 2014-09-17 2017-09-26 拜耳作物科学有限合伙公司 包含重组芽孢杆菌属细胞和其他生物防治试剂的组合物
CN107794239A (zh) * 2017-11-15 2018-03-13 云南省烟草农业科学研究院 一种东洋芽孢杆菌菌株以及菌剂制备方法和应用
CN114149942A (zh) * 2021-11-10 2022-03-08 四川农业大学 一株东洋芽孢杆菌及其应用
CN117683678A (zh) * 2023-12-13 2024-03-12 青岛和协生物科技有限公司 一种具有防治甘蓝蚜虫效果的东洋芽孢杆菌

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107205404A (zh) * 2014-09-17 2017-09-26 拜耳作物科学有限合伙公司 包含重组芽孢杆菌属细胞和其他生物防治试剂的组合物
CN106854631A (zh) * 2016-12-26 2017-06-16 青岛蔚蓝生物集团有限公司 一种植物病害的微生物防治方法
CN107794239A (zh) * 2017-11-15 2018-03-13 云南省烟草农业科学研究院 一种东洋芽孢杆菌菌株以及菌剂制备方法和应用
CN114149942A (zh) * 2021-11-10 2022-03-08 四川农业大学 一株东洋芽孢杆菌及其应用
CN117683678A (zh) * 2023-12-13 2024-03-12 青岛和协生物科技有限公司 一种具有防治甘蓝蚜虫效果的东洋芽孢杆菌

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SAUKA DIEGO HERMAN; PERALTA CECILIA; PÉREZ MELISA PAULA; ONCO MARÍA INÉS; FIODOR ANGELIKA; CABALLERO JAVIER; CABALLERO PRIMITIVO; : "Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests", BIOLOGICAL CONTROL, SAN DIEGO, CA, US, vol. 167, 21 January 2022 (2022-01-21), US , XP086963975, ISSN: 1049-9644, DOI: 10.1016/j.biocontrol.2022.104838 *

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