WO2025133003A3 - Lactoglobuline bêta bovine non animale - Google Patents

Lactoglobuline bêta bovine non animale

Info

Publication number
WO2025133003A3
WO2025133003A3 PCT/EP2024/087733 EP2024087733W WO2025133003A3 WO 2025133003 A3 WO2025133003 A3 WO 2025133003A3 EP 2024087733 W EP2024087733 W EP 2024087733W WO 2025133003 A3 WO2025133003 A3 WO 2025133003A3
Authority
WO
WIPO (PCT)
Prior art keywords
blg
modified
beta lactoglobulin
bovine beta
essential
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2024/087733
Other languages
English (en)
Other versions
WO2025133003A2 (fr
Inventor
José Arnau MARTINEZ
Jan Lehmbeck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
21stBio AS
Original Assignee
21stBio AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 21stBio AS filed Critical 21stBio AS
Publication of WO2025133003A2 publication Critical patent/WO2025133003A2/fr
Publication of WO2025133003A3 publication Critical patent/WO2025133003A3/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4717—Plasma globulins, lactoglobulin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/69—Aspergillus oryzae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente divulgation concerne une cellule hôte microbienne génétiquement modifiée exprimant un ou plusieurs gènes hétérologues codant pour une bêta-lactoglobuline modifiée (BLG), produisant ainsi ladite BLG modifiée, la BLG modifiée comprenant au moins une substitution d'un acide aminé non essentiel et/ou non ramifié avec un acide aminé essentiel et/ou ramifié par rapport à une BLG non modifiée parente.
PCT/EP2024/087733 2023-12-19 2024-12-19 Lactoglobuline bêta bovine non animale Pending WO2025133003A2 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP23218277.4 2023-12-19
EP23218277 2023-12-19
EP24163561 2024-03-14
EP24163561.4 2024-03-14
EP24198998.7 2024-09-06
EP24198998 2024-09-06

Publications (2)

Publication Number Publication Date
WO2025133003A2 WO2025133003A2 (fr) 2025-06-26
WO2025133003A3 true WO2025133003A3 (fr) 2025-10-02

Family

ID=94227696

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2024/087733 Pending WO2025133003A2 (fr) 2023-12-19 2024-12-19 Lactoglobuline bêta bovine non animale

Country Status (1)

Country Link
WO (1) WO2025133003A2 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021168343A2 (fr) * 2020-02-19 2021-08-26 Perfect Day, Inc. Protéines de lait recombinantes hypoallergéniques et compositions les comprenant
WO2023122280A2 (fr) * 2021-12-23 2023-06-29 Perfect Day, Inc. Compositions et procédés de traitement de défauts cutanés
WO2023150696A2 (fr) * 2022-02-03 2023-08-10 Perfect Day, Inc. Compositions et procédés d'administration d'agents bioactifs hydrophobes

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
AU1774000A (en) 1998-12-23 2000-07-31 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells
EP1756273B1 (fr) 2004-06-14 2009-10-07 Novozymes A/S Peptide signal pour production d'un polypeptide
EP2059590B1 (fr) 2006-07-14 2014-12-31 Novozymes, Inc. Procédés de production de polypeptides secrétés présentant une activité biologique
EP2527448A1 (fr) 2011-05-23 2012-11-28 Novozymes A/S Intégrations simultanées spécifiques au site de copies de plusieurs gènes dans des champignons filamenteux
JP6449763B2 (ja) 2012-05-31 2019-01-09 ノボザイムス アクティーゼルスカブ 真菌における改善された選択
US10619144B2 (en) 2013-08-23 2020-04-14 Novozymes A/S Regulated PepC expression
US12516362B2 (en) 2018-11-28 2026-01-06 Novozymes A/S Filamentous fungal host cells
KR20220010499A (ko) 2019-04-22 2022-01-25 퍼펙트 데이, 인크. 재조합 우유 단백질 및 그를 포함하는 조성물

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021168343A2 (fr) * 2020-02-19 2021-08-26 Perfect Day, Inc. Protéines de lait recombinantes hypoallergéniques et compositions les comprenant
WO2023122280A2 (fr) * 2021-12-23 2023-06-29 Perfect Day, Inc. Compositions et procédés de traitement de défauts cutanés
WO2023150696A2 (fr) * 2022-02-03 2023-08-10 Perfect Day, Inc. Compositions et procédés d'administration d'agents bioactifs hydrophobes

Also Published As

Publication number Publication date
WO2025133003A2 (fr) 2025-06-26

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