WO2026035233A2 - Biostimulant dérivé d'acide aminé animal à partir de fibre de laine et son procédé de production - Google Patents

Biostimulant dérivé d'acide aminé animal à partir de fibre de laine et son procédé de production

Info

Publication number
WO2026035233A2
WO2026035233A2 PCT/TR2025/050918 TR2025050918W WO2026035233A2 WO 2026035233 A2 WO2026035233 A2 WO 2026035233A2 TR 2025050918 W TR2025050918 W TR 2025050918W WO 2026035233 A2 WO2026035233 A2 WO 2026035233A2
Authority
WO
WIPO (PCT)
Prior art keywords
biostimulant
plant
hydrolyzate
filtering
preparing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/TR2025/050918
Other languages
English (en)
Other versions
WO2026035233A3 (fr
Inventor
Arife Candaş ADIGÜZEL ZENGİN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ege Universitesi
Original Assignee
Ege Universitesi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ege Universitesi filed Critical Ege Universitesi
Publication of WO2026035233A2 publication Critical patent/WO2026035233A2/fr
Publication of WO2026035233A3 publication Critical patent/WO2026035233A3/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F1/00—Fertilisers made from animal corpses, or parts thereof
    • C05F1/005—Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/10—Fertilisers containing plant vitamins or hormones

Definitions

  • the invention relates to an animal amino acid-derived biostimulant obtained by utilizing wool fiber waste generated from leather production for use in the field of agriculture and the production method thereof.
  • the biostimulant of the invention is used to reduce the stress factor that plants encounter due to environmental factors that influence their growth and development, as well as to address issues caused by drought and salinity, especially in irrigated agriculture.
  • Abiotic stress is a condition that is induced by physical or chemical factors that limit the ability of plants to adapt to environmental conditions. These stress factors adversely affect the physiological and biochemical processes of plants and consequently can have negative effects on growth, development, yield, and general health.
  • Water stress one of the abiotic stress factors, exposes plants to stress in regions with inadequate or uneven water distribution. Over-irrigation also leads to this stress. Another factor is salinity, and high salt concentrations in the soil are also a source of stress for plants. This negatively affects the water and nutrient uptake of plants. In addition, high or low temperatures also cause stress for plants. Temperature changes negatively affect plant metabolism and fundamental processes such as photosynthesis. These abiotic stressors, such as drought, salinity, and temperature extremes, are currently causing massive crop losses globally. Plants face various abiotic stressors throughout their life cycle that negatively affect their growth and production. Globally, these stresses reduce annual crop yields by up to 70% and cause malnutrition as well as food shortages for the growing world population.
  • Biostimulants are defined as any substance or microorganism applied to plants to enhance growth, nutrition, product quality, and yield, and to increase the resistance of plants to abiotic stress while being distinct from the nutritional content of the product and facilitating nutritional processes. Plant biostimulants are also referred to as products containing mixtures of such substances and/or microorganisms [3,4,5].
  • plant biostimulants are classified into following groups: (i) humic and fulvic acids, (ii) animal and plant-based protein hydrolysates, (iii) macroalgae seaweed extracts, and (iv) silicon along with beneficial microorganisms [4],
  • Biostimulants are substances that can be applied to various plant parts including seeds, leaves, and soil, and some biostimulants also possess the ability to regulate soil structure [3].
  • biostimulants are natural products of plant, animal, or microorganism origin that, when applied in small quantities to plants (leaves or rhizosphere), stimulate natural processes that enhance growth, crop quality, nutrient utilization efficiency, and tolerance to abiotic stress.
  • biostimulants allows for a reduced use of agrochemicals (especially fertilizers) in agriculture without compromising crop productivity and quality, while also providing protection against abiotic stress.
  • the use of biostimulants does not have to directly provide nutrients to plants or target pathogens but instead regulates physiological processes, making it possible to increase plant growth and improve tolerance to abiotic stress [6].
  • PBB Protein-based biostimulants
  • PBB products are mainly mixtures of peptides and amino acids and most PBB products are derived from protein-rich plant and animal derived substances that have been enzymatically or chemically treated or subjected to thermal hydrolysis [7], For this reason, these products are often referred to as protein hydrolyzates (PHs).
  • Peptides contain free essential and non-essential amino acids in different amounts depending on the protein source, the processing methods used, and the degree of hydrolysis. These active components (peptides and amino acids) in PHs contribute to increased uptake of beneficial elements into plant tissues through leaves or roots [8].
  • chelating and complexing activities of certain amino acids and peptides enhance the mobility of micronutrients, improve nutrient availability, and facilitate uptake by roots through chelation.
  • some nitrogen compounds including the amino acids glycine, betaine and proline, provide antioxidant activity by scavenging free radicals, which contributes to the reduction of environmental stress [3].
  • PHs are also known to increase microbial biomass and activity, soil respiration, and soil fertility in general.
  • Plant-derived biostimulants are organic or inorganic compounds used to enhance plant growth, development and productivity.
  • biostimulants are usually given to plants as seed, foliar, or root applications.
  • hormones regulating the growth of plants are used as biostimulants.
  • plant growth hormones such as cytokinins, auxins, and gibberellins promote plant growth and increase their ability to cope with stress.
  • Fulvic and humic acid improve soil structure, enhance nutrient absorption and promote plant growth, as well as improving soil fertility by increasing the activity of microorganisms in the soil.
  • Microorganisms such as nitrogen-fixing, phosphate solubilizing bacteria promote plant growth through better absorption of plant nutrients.
  • some plant extracts strengthen the defense system of the plants and increase their resistance to diseases.
  • biostimulants are more expensive than chemical fertilizers, which can create additional costs for agricultural enterprises and increase production costs.
  • biostimulants raise concerns about their environmental impact, as some biostimulants, such as seaweed extracts, are harvested from marine ecosystems and excessive harvesting from such sources can upset the environmental balance.
  • the invention describes an animal amino acid-derived biostimulant obtained by utilizing wool fiber waste generated from leather production for use in the field of agriculture and the production method thereof.
  • the biostimulant of the invention is used to reduce the stress factor that plants encounter due to environmental factors that influence their growth and development, as well as to address issues caused by drought and salinity, especially in irrigated agriculture.
  • the object of the invention is to provide a plant biostimulant that provides a rapid effect on the growth mechanism in the development of plants and provides a response in the shortest time.
  • the biostimulant of the invention has a high content of animal amino acids and thus has a rapid effect on plant growth.
  • Another object of the invention is to obtain a plant biostimulant with an environmentally friendly approach and low cost.
  • the biostimulant of the invention is obtained from wool fiber produced as waste in leather production. In this way, waste materials are recycled by being used as inputs in biostimulant production, and also production can be realized at a low cost.
  • the invention relates to an animal amino acid-derived biostimulant obtained by utilizing wool fiber waste generated from leather production for use in the field of agriculture, and the production method thereof.
  • the biostimulant of the invention can be applied to plants from seed, soil, and leaves. Said biostimulant is used in the elimination of the abiotic stress factors experienced by plants due to environmental factors arising in their growth and development, in the solution of abiotic stress problems of the plants caused by drought and salinity, especially in irrigated agriculture.
  • the biostimulant of the invention comprises 10-15 wt.% animal-derived free amino acids, bound amino acids, macro- and micropeptides of animal-derived keratin protein, and water. In an embodiment of the invention, said biostimulant comprises 11% free amino acids.
  • the biostimulant of the invention containing animal amino acids comprises 14 different free form amino acids (GLU; glutamic acid, SER; sericin, HIS; histamine, GLY; glycine, ALA; alanine, TYR; tyrosine, CYS; cysteine, VAL; valine, MET; methionine, RHE; phenylalanine, ILE; isoleucine, LEU; leucine, LYS; lysine, PRO; proline).
  • GLU free form amino acids
  • the free amino acids with the highest ratios in this composition are PRO (1-5 wt.%, optimum 2.5%), GLY (0.75-4 wt.%, optimum 2.5%), ALA (0.5-3 wt.%, optimum 2.5%) and GLU (0.25-3 wt.%, optimum 1 .5%).
  • the biostimulant of the invention is obtained by hydrolyzing the raw material (wool) in protein structure under high temperature and chemical effect (alkali). After the completion of the hydrolyzation period, the product brought to a neutral pH value is filtered with filter paper and then the number of amino acids contained therein is analyzed using instrumental methods (HPLC device).
  • the production method of the plant biostimulant of the invention comprises the process steps of: i. cleaning, washing, drying, and preparing waste wool fibers for hydrolysis, ii. preparing and delivering sodium hydroxide (NaOH) to the hydrolyzation tanks together with the waste wool fibers, iii. performing the hydrolyzation process of waste wool fiber and bringing it to room temperature, iv. filtering the hydrolyzate with filter paper, v. adjusting the filtrate to a neutral pH value (pH:7), vi. re-filtering the hydrolyzate with filter paper.
  • NaOH sodium hydroxide
  • the production method of the plant biostimulant of the invention comprises the process steps of: i. cleaning, washing, drying, and preparing 5- 50 grams of waste wool fibers for hydrolysis, ii. preparing and delivering 0.5- 2.5 N sodium hydroxide (NaOH) to the hydrolysis tanks together with the waste wool fibers and bringing it to room temperature, iii. performing hydrolyzation process at a process temperature of 70-100°C for a process time of 5-24 hours, iv. filtering the hydrolyzate with filter paper, v. adjusting the filtrate to a neutral pH value (pH:7), vi. re-filtering the hydrolyzate with filter paper.
  • NaOH sodium hydroxide
  • the production method of the plant biostimulant of the invention comprises the process steps of: i. cleaning, washing, drying, and preparing 15 grams of waste wool fibers for hydrolysis, ii. preparing and delivering 1 .5 N sodium hydroxide (NaOH) to the hydrolysis tanks together with the waste wool fibers and bringing it to room temperature, iii. performing hydrolyzation process at a process temperature of 90°C for a process time of 10 hours, iv. filtering the hydrolyzate with filter paper, v. adjusting the filtrate to a neutral pH value (pH:7), vi. re-filtering the hydrolyzate with filter paper.
  • NaOH sodium hydroxide
  • the invention relates to an animal amino acid-derived biostimulant obtained by utilizing wool fiber waste generated from leather production for use in the field of agriculture, particularly dry agriculture, and to a production method thereof, and is industrially applicable.
  • Muge.Misir (Zea mays. L.) bitkisinin gimlenme ve fide dbnemlerinde uygulanan kitosanm fizyolojik ve morfolojik ozellikler uzerine etkisi. 2023. Master's Thesis.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Abstract

L'invention concerne un biostimulant dérivé d'acide aminé animal obtenu en utilisant des déchets de fibre de laine générés à partir de la production de cuir pour une utilisation dans le domaine de l'agriculture, et son procédé de production. Le biostimulant de l'invention est produit selon un procédé d'hydrolyse alcaline. Ledit biostimulant est utilisé pour soutenir la croissance et le développement de plantes contre des facteurs de stress abiotiques, et pour résoudre des problèmes induits par la sécheresse et la salinité, en particulier dans l'agriculture sèche.
PCT/TR2025/050918 2024-08-07 2025-08-06 Biostimulant dérivé d'acide aminé animal à partir de fibre de laine et son procédé de production Pending WO2026035233A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2024/010295A TR2024010295A2 (tr) 2024-08-07 2024-08-07 Yün li̇fi̇nden hayvansal ami̇noasi̇t kaynakli bi̇yosti̇mülant ve bunun üreti̇m yöntemi̇
TR2024/010295 2024-08-07

Publications (2)

Publication Number Publication Date
WO2026035233A2 true WO2026035233A2 (fr) 2026-02-12
WO2026035233A3 WO2026035233A3 (fr) 2026-04-30

Family

ID=98275098

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/TR2025/050918 Pending WO2026035233A2 (fr) 2024-08-07 2025-08-06 Biostimulant dérivé d'acide aminé animal à partir de fibre de laine et son procédé de production

Country Status (2)

Country Link
TR (1) TR2024010295A2 (fr)
WO (1) WO2026035233A2 (fr)

Also Published As

Publication number Publication date
TR2024010295A2 (tr) 2024-08-21
WO2026035233A3 (fr) 2026-04-30

Similar Documents

Publication Publication Date Title
AU2014101391A4 (en) Multifunctional organic agricultural fertilizer composition and process for preparation thereof
Mady Effect of foliar application with yeast extract and zinc on fruit setting and yield of faba bean (Vicia faba L.)
US11716991B2 (en) Biostimulants for enhancing crop productivity, methods and uses thereof
KR20120099873A (ko) 친환경 맞춤형 작물 근권분해 효소 복합체
TWI865152B (zh) 熱力學水解之羽毛角蛋白水解胜肽液用於提昇小麥抗乾旱逆境之用途
De Lucia et al. Type of bio-stimulant and application method effects on stem quality and root system growth in LA Lily
Salihi et al. Impacts of humic acid on growth and yield of wheat: A review
Wani et al. Role of foliar sprays in sericulture industry
CN118084561A (zh) 一种农用酵母源氨基酸多肽制剂、其制备工艺及其应用
CN103907423A (zh) 小球藻有机大米培育方法
US11040919B2 (en) Fertilizing composition based on soy hydrolysates
Sonkar et al. Role of protein hydrolysates in plants growth and development
Gangaiah et al. Modern crop management practices for pearl millet cultivation in Asia
Saleh et al. An attempt for reducing mineral fertilization in lettuce production by using bio-organic farming system
US20230127060A1 (en) Urea cycle augmenting composition and methods of use
WO2024200873A1 (fr) Composition comprenant un hydrolysat protéique et un extrait de microalgues et son utilisation en tant que biostimulant agricole
CA2473787C (fr) Technique relative a la production d'un produit unique d'addition pour sols pour l'horticulture et l'agronomie
Hongal et al. Bio-Stimulants in Oil Seed Crops-Its Influence on Growth, Yield and Quality.
US11293006B1 (en) Endophytes for organic nitrogen use for sustainable agriculture
TR2024010295A2 (tr) Yün li̇fi̇nden hayvansal ami̇noasi̇t kaynakli bi̇yosti̇mülant ve bunun üreti̇m yöntemi̇
Calinescu et al. Organic fertilization influence on growth and fruiting processes of three apple cultivars grown in the maracineni-arges area
RU2858079C1 (ru) Органоминеральное удобрение
Kołodziejczyk et al. The effect of fertilizers containing free amino acids on the yield of modern and old common wheat cultivars in organic production
Abd El-Rheem et al. Response of lettuce plants to foliar application of amino acids under different rates of vermicompost
CA3248676A1 (fr) Biostimulants biocatalysés stimulant le développement de système de racines de plantes, leurs procédés et leurs utilisations