WO2026039155A2 - Systèmes et procédés de détection rapide et autonome de particules d'aérosol - Google Patents

Systèmes et procédés de détection rapide et autonome de particules d'aérosol

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Publication number
WO2026039155A2
WO2026039155A2 PCT/US2025/038725 US2025038725W WO2026039155A2 WO 2026039155 A2 WO2026039155 A2 WO 2026039155A2 US 2025038725 W US2025038725 W US 2025038725W WO 2026039155 A2 WO2026039155 A2 WO 2026039155A2
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WO
WIPO (PCT)
Prior art keywords
sample
station
disk
sample disk
aerosol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2025/038725
Other languages
English (en)
Inventor
Michael Mcloughlin
Nathaniel K. JONES
Daniel Jeffrey Gussin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zeteo Tech Inc
Original Assignee
Zeteo Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US18/780,251 external-priority patent/US20240379337A1/en
Application filed by Zeteo Tech Inc filed Critical Zeteo Tech Inc
Publication of WO2026039155A2 publication Critical patent/WO2026039155A2/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/161—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission using photoionisation, e.g. by laser
    • H01J49/164—Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI]

Definitions

  • This disclosure relates to systems and methods that use mass spectrometry and optionaly one or more optical techniques to provide high accuracy, rapid and autonomous identification of aerosol analyte particles. More particularly, but not by way of limitation, the present disclosure relates to systems and and methods for identifying biological and chemical aerosol analytes using matrix assisted laser desorption mass spectrometry (“MALDI-MS”) using time-of-flight mass spectrometry (TOFMS).
  • MALDI-MS matrix assisted laser desorption mass spectrometry
  • TOFMS time-of-flight mass spectrometry
  • an autonomous aerosol threat identifier should collect and analyze a sample and determine if the aerosol is hazardous in less than about five minutes. If aerosol threats can be identified in less than about five minutes, remedial actions can be taken to limit the spread of aerosol within a building such as an airport terminal building. As more time lapses, the aerosol is spread by the movement of people and, more significantly, by the normal functioning of a building’s ventilation system. In tall buildings, elevators can also move aerosol throughout the building in a matter of minutes.
  • Another solution is target specific and works only for bacterial analytes at the expense of analyzing viruses, toxins, or particulate chemicals.
  • This method requires a sample, for example from a patient, to be applied to a bacterial culture plate and incubated for 8 to 24 hours. After the bacterial colonies have grown, individual amplified and purified colonies are collected and measured by whole cell “matrix assisted” laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry.
  • MALDI matrix assisted laser desorption ionization
  • TOF time-of-flight
  • U.S. Pat. No. 8,441,632 titled “BIOLOGICAL AND CHEMICAL MICROSCOPIC TARGETING” discloses a technique that is potentially both sensitive, specific and rapid (approximately five minutes or less), but does not employ target-specific reagents, and is based on RAMAN spectroscopy.
  • Raman spectroscopy is an analysis technique used to determine vibrational modes of molecules, although rotational and other low-frequency modes may also be observed. It is based upon the interaction of light with the chemical bonds within a material.
  • RAMAN spectroscopy when applied to microbial samples does not sufficiently resolve near neighbors in a genus or species where some members of the group are pathogenic to humans and some are not.
  • the target particle (analyte) is coated by or mixed with a matrix chemical.
  • the sample mixture is then allowed to dry prior to being loaded into the mass spectrometer.
  • the matrix chemical preferentially absorbs light (often ultraviolet wavelengths) from a short, intense laser pulse.
  • the biological molecules would have a propensity to decompose by pyrolysis when exposed to intense ultraviolet light.
  • the laser energy is preferentially absorbed by the matrix chemical, causing the matrix and the analyte to be vaporized.
  • the matrix chemical also transfers charge to the vaporized molecules, creating ions that are then accelerated down a flight tube by the electric field.
  • the coated analyte particles which are often intact microbes, are then analyzed using MALDI Time of Flight (TOF) mass spectrometry (MS).
  • TOF Time of Flight
  • a liquid usually included an acid, such as tri-fluoro-acetic acid (TFA), and a MALDI matrix chemical such as alpha-cyano-4-hydroxycinnamic acid, is dissolved in a solvent and added to the analyte.
  • Solvents include acetonitrile, water, methanol, ethanol, and acetone.
  • TFA is normally added to suppress the influence of salt impurities on the mass spectrum of the analyte and to leach acid-soluble proteins from within the analyte.
  • the acid partially degrades the cell membrane of the analyte making the proteins available for ionization and analysis in a TOF mass spectrometer.
  • Water enables hydrophilic proteins to dissolve, and methanol enables at least some hydrophobic proteins to dissolve.
  • the MALDI matrix solution is spotted on to the analyte on a MALDI plate to yield a uniform homogenous layer of MALDI matrix material on the analyte.
  • the solvents vaporize, leaving only the recrystallized matrix with the analyte spread through the matrix crystals.
  • the coated plate containing the analyte mixed with acid and matrix is then analyzed in a TOF mass spectrometer.
  • Other MALDI matrix materials include 3,5-dimethoxy-4-hydroxycinnamic acid (sinapinic acid), a-cyano-4- hydroxycinnamic acid (a-cyano or a-matrix) and 2,5-dihydroxybenzoic acid (DHB) as described in U.S. Pat. No. 8,409,870.
  • the MALDI technique coupled with high- mass-range time-of- flight (TOF) mass spectrometry may also permit direct analysis of large peptide components, and complete proteins enabling “whole cell” biological
  • U.S. Pat. Pub. No. 2003/0020011 titled “SAMPLE COLLECTION PREPARATION METHODS FOR TIME-OF FLIGHT MINIATURE MASS SPECTROMETER,” discloses a method and device for collecting ambient aerosols such as biological hazards and identifying the composition of the aerosol particles.
  • a MALDI matrix chemical is nebulized and injected into an ambient sample aerosol.
  • the matrix aerosol particles and sample aerosol particles are co-deposited on medium such as a VCR (video cassette recorder) tape.
  • the tape is then moved into a MALDI time-of-flight mass spectrometer for analysis.
  • the matrix particle and ambient aerosol particle interactions occur on the tape because the particles do not collide with each other prior to deposition on the tape.
  • U.S. Pat No. 6,841,773 titled “PORTABLE TIME OF FLIGHT MASS SPECTROMETER SYSTEM” discloses a field portable mass spectrometer system including a sample collector and a sample transporter in the form of a video cassette tape. The sample transporter interfaces with the sample collector to receive sample deposits thereon.
  • the system includes a time of flight (TOF) mass spectrometer.
  • TOF time of flight
  • U.S. Pat. Pub. No. 2005/0017102 titled “ELECTROSTATIC ATOMIZER AND METHOD OF PRODUCING ATOMIZED FLUID SPRAYS,” discloses contacting an analyte sample stream with one or more spray fluids contained in separate reservoirs including a MALDI matrix, depositing the analyte on a substrate such as a MALDI plate and analyzing using MALDI MS.
  • a first microinjector is fed with isopropanol.
  • a second injector is fed with a mixture of 70% acetonitrile, 30% water and a third injector 0.1% trifluoroacetic acid.
  • a third injector is fed sequentially with various process fluids, including water, water/glycerine, acetic acid, formic acid, and ethanol.
  • the controlled microinjectors e.g., 100
  • the injectors are oriented such that each injector sprays into the same region of the sample preparation zone.
  • the sample preparation zone is a channel, perpendicular to the orientation of the microinjectors.
  • a concentrated gas stream containing the sample to be analyzed such as bacteria spores or other biological materials, is passed through the sample preparation zone, contacted with the sprayed fluids, and then directed onto a sample slide for MALDI analysis.
  • U.S. Pat. No. 7,125,437 titled “METHOD AND APPARATUS FOR ENHANCED PARTICLE COLLECTION EFFICIENCY,” discloses a method for particle collection including the steps of guiding an air stream containing particles to be collected and analyzed toward an impaction surface and introducing an aerosol containing aqueous droplets into the air stream upstream from the impaction surface to coagulate the particles with the aqueous droplets and to increase a size of the particles enhancing a collection efficiency of the coagulated particles on the impaction surface.
  • the aerosol containing the aqueous droplets may be produced by directing a pressurized stream of carrier gas against a reservoir containing a liquid, thereby aspirating the liquid out of the reservoir while shearing liquid particulates apart to aerosolize the aspirated liquid before co-aerosolizing the particles in the air stream.
  • the aerosol containing the aqueous droplets may be produced by driving a selected liquid through a piezo-electric based nebulizer including a piezoelectric element and vibrating the piezo-electric element at a desired frequency sufficient to shear apart liquid particulates to generate the aerosol as the liquid particulates traverse an outlet of the piezo-electric based element.
  • the disclosed method may further include the steps of wetting the impaction surface as the coagulated particles impinge against the impaction surface to form a pool of liquid on the impaction surface to minimize bouncing of the coagulated particle off the impaction surface to enhance the collection efficiency, and selecting additives and coaerosolizing the selected additives to create an environment in the air stream or in the pool of liquid on the impaction surface to mechanically, chemically or biologically modify the coagulated particles to enhance identification of the collected coagulated particles.
  • the additives are selected that the environment created in the air stream or in the pool of liquid allows accelerated growth of organisms to be cultured.
  • Formation of the pool of standing liquid droplets can also be realized by introducing a metered volume of liquid directly in the vicinity of the impaction surface, for example, at the base of an impaction nozzle.
  • a metered volume of liquid directly in the vicinity of the impaction surface, for example, at the base of an impaction nozzle.
  • the pool of standing droplets can be created right on this surface.
  • the methods and apparatus disclosed in U.S. Pat. No. 7,125,437 and U.S. Pat. No. 6,841,773 do not result in a reliable aerosol sampling and matrix mixing apparatus for MALDI-MS analysis because aerosolizing the MALDI matrix using the methods described tends to crystallize a fraction of the matrix chemical and clog the nebulizer.
  • an example sample capture and analysis system for analyzing aerosol analyte particles in air may include a fresh sample disk station or substrate loader station configured to receive a fresh disk cartridge having one or more fresh sample disks, a spent sample disk unloader station, an aerosol sample collection station, and a sample disk holder including a stub.
  • the sample disk holder may be configured to removably engage with the fresh disk cartridge to receive a fresh sample disk, removably engage with the spent sample disk unloader station to return a spent sample disk, hold a fresh sample disk or a spent sample disk, and move in at least two directions (X-Y-Z) orthogonal to each other using one or more of a stepper motor or actuator using a predetermined analysis sequence, and one or more analysis stations.
  • the aerosol sample collection station may be configured to produce a sample spot on a fresh sample disk when the sample disk holder is positioned at the aerosol sample collection station.
  • the example system may further include a microcontroller configured to run the predetermined analysis sequence.
  • the aerosol sample collection station may include an impactor nozzle having a nozzle tip disposed at a predetermined spacing above the fresh sample disk. Air including the analyte particles may be drawn through the impactor nozzle at a predetermined air flow rate to produce a sample spot on the fresh sample disk.
  • the spent sample disk unloader station may include a spent sample disk storage container and means to disengage the spent sample disk from the sample disk holder and transfer it to the storage container.
  • the spent sample disk unloader station may include a spent sample disk cartridge configured to removably engage with the spent disk cartridge and receive a spent sample disk.
  • an example sample capture and analysis system for analyzing aerosol analyte particles in air may further include a particle counts sensor configured to determine an anomaly in the composition of ambient air.
  • the particle counts sensor may be configured to output one or more of particle size distribution, mean particle diameter, total particle count per unit volume, fluorescence of particles, depolarization properties of the aerosol particles, particle velocity distribution, or target analyte to clutter particle ratio.
  • the stub may be made of an electrically conductive material.
  • the sample disks may be made of one or more of nickel or nickel alloys. The sample disks may be pre-coated with a MALDI matrix chemical.
  • an example sample capture and analysis system for analyzing aerosol analyte particles in air may further include one or more of a camera station, a liquid chemical dispensing station, or a drying station.
  • Each sample disk may include a unique identifier including one or more of a bar code, QR code (2- dimensional bar code), a numeric string, an alphanumeric string, or micro-identifier dots. The unique identifier of each disk may be read using a camera or a unique identifier reader.
  • the dispensing station may be configured to dispense between about 0.5 pl and about 2 pl of a liquid.
  • the liquid dispensed may include one or more of a MALDI matrix chemical, TFA, acetic acid, formic acid, acetonitrile, methanol, ethanol, or water.
  • the one or more analysis stations may include one or more of a TOFMS, LDI-MS, MALDLTOFMS, LIBS, Raman spectroscopy, fluorescence microscopy, surface enhanced RAMAN spectroscopy, scanning electron microscopy IR spectroscopy, or an optical detector.
  • the camera station may be configured to receive at least one of a microscope camera and a digital camera.
  • the drying station may be configured to substantially dry the sample using one or more of inductive heating, resistive heating, flow of air, or vacuum, or combinations thereof.
  • an example method for collecting and analyzing aerosol analyte sample particles in air may include providing any one of the example sample capture and analysis systems previously disclosed herein, loading a fresh sample disk onto the sample disk holder at the fresh sample disk loader station, moving the sample disk holder having a fresh disk to the aerosol sample collection station, wherein aerosol particles are impacted onto the sample disk to produce a sample spot size on the sample disk.
  • the sample spot may be about 1 mm in diameter.
  • the example method may further include moving the sample disk holder to the camera station and examining the sample spot, moving the sample disk holder to the liquid chemical dispensing station for treating the deposited aerosol particles with chemicals, and substantially drying the sample.
  • the example method may include moving the sample disk holder to the one or more analysis stations and analyzing the sample.
  • Each sample disk may include a unique identifier including one or more of a bar code, QR code (2-dimensional bar code), a numeric string, an alphanumeric string, or micro-identifier dots.
  • the example method may further include the step of moving the sample disk holder to the spent sample disk unloader station to unload the spent sample disk from the sample disk holder.
  • the step of moving the sample disk holder having a fresh disk to the aerosol collection station step may be triggered by an anomaly in ambient air determined by the output of a particle counts sensor. In some implementations, the step of moving the sample disk holder having a fresh disk to the aerosol collection station step may be triggered by one or more of a predetermined sampling schedule, or a manual command.
  • an example method for collecting and analyzing aerosol analyte sample particles in air may further include the step of tuning one or more of a sample collection period or a flow rate of air through an impactor disposed at the sample collection station based on the output of a particle counts sensor.
  • the flow rate of air drawn through the impactor nozzle may be inversely proportional to the mean particle diameter of the analyte particles in air.
  • the sample collection period may be inversely proportional to the total particle count of the analyte particles per unit volume of air measured using a particle counts sensor.
  • an example method for collecting and analyzing aerosol analyte sample particles in air may further include the step of reading the fresh sample disk unique identifier using one or more of a camera or a unique identifier reader to associate the unique identifier of the fresh disk with the step of analyzing the sample corresponding to that disk.
  • the analyzing the sample step may include analyzing the sample using TOFMS.
  • an example method for collecting and analyzing aerosol analyte sample particles in air may further include the steps of generating TOFMS raw spectral data unique to the aerosol analyte particles, performing one or more of filtering, baseline subtraction, signal to noise ratio estimation, peak detection, or feature extraction to generate processed spectral data corresponding to the first set and second set of raw spectral data, and identifying the composition of the aerosol analyte particle by comparing the processed spectral data with a reference library including one or more of processed spectral data of several biological or chemical analytes.
  • the step of generating TOFMS raw spectral data unique to the aerosol analyte particles may include generating a first set of raw spectral data by subjecting the sample to a first batch of laser ionization pulses, the first batch of laser pulses characterized by a first laser pulse energy (microjoules per pulse), and generating a second set of raw spectral data by subjecting the sample to a second batch of laser ionization pulses, the second batch of laser pulses characterized by second laser pulse energy (microjoules per pulse).
  • the second laser pulse energy may be greater than the first laser pulse energy.
  • the first batch of laser ionization pulses may be characterized by a laser pulse energy of about 5 microjoules per pulse.
  • the second batch of laser ionization pulses may be characterized by a laser pulse energy of between about 15 microjoules per pulse and 25 microjoules per pulse.
  • the number of laser ionization pulses in one or more of the first batch or second batch is between about 1 and about 200.
  • generating TOFMS raw spectral data unique to the aerosol analyte particles may include generating a set of raw spectral data associated with ionization of the aerosol analyte particles in the sample spot using a plurality of batches of ionization pulses, wherein each batch includes a predetermined number of laser ionization pulses, and wherein each batch of ionization pulses is associated with a respective parameter set including one or more of ionization energy, pulse delay, applied voltage, or calibration data.
  • the ionization energy associated with a batch of laser ionization pulses may progressively increases with each subsequent batch of ionization pulses.
  • an example method for collecting and analyzing aerosol analyte sample particles in air may further include the step of generating one or more data files corresponding to the raw spectral data or processed spectral data and associating the one or more data files with one or more of a date stamp of the analyzing step and the unique identifier of the sample disk.
  • An example method for collecting and analyzing aerosol analyte sample particles in air may further include the step of transferring the one or more data files corresponding to the raw spectral data or processed spectral data to one or more of a local data server, a local data analysis server, a cloud-based data server, or a cloud-based data analysis server.
  • an example method for collecting and analyzing aerosol analyte sample particles in air may further include the steps of generating raw spectral data unique to the aerosol analyte particles, performing one or more of filtering, baseline subtraction, signal to noise ratio estimation, peak detection, or feature extraction to generate processed spectral data and identifying the composition of the aerosol analyte particle by comparing the processed spectral data with a reference library including one or more of processed spectral data of several biological or chemical analytes.
  • an example sample capture and analysis system for analyzing analyte particles in a liquid sample may include a fresh sample disk station or substrate loader station configured to receive a fresh disk cartridge having one or more fresh sample disks, a spent sample disk unloader station, a liquid sample acceptance station configured to receive the liquid sample, a liquid chemical dispensing station, a sample disk holder including a stub.
  • the sample disk holder may be configured to removably engage with the fresh sample disk cartridge to receive a fresh sample disk, removably engage with the spent sample disk unloader station to return a spent sample disk, hold a fresh sample disk or a spent sample disk, and move in at least two directions (X-Y-Z) orthogonal to each other using one or more of a stepper motor or actuator using a predetermined analysis sequence.
  • An example sample capture and analysis system for analyzing analyte particles in a liquid sample may include one or more analysis stations.
  • the liquid sample acceptance station may be configured to produce a sample spot on a fresh sample disk.
  • the operation of the system may be controlled using a microcontroller configured to run the predetermined analysis sequence.
  • the liquid sample acceptance station may be configured to receive a liquid sample from an aerosol collection device.
  • the liquid sample acceptance station may be configured to receive a liquid sample including exhaled breath.
  • the liquid sample acceptance station may be configured to receive a liquid sample obtained from a liquid sample processing device capable of at least one of purifying, digesting, and concentrating target analytes.
  • the aerosol collection device may include one or more of an impactor or a liquid impinger.
  • the liquid sample acceptance station may be configured to receive one or more of a solution or suspension of a powder or a pill.
  • a sample capture and analysis system for analyzing aerosol analyte particles may further include a rotation motor configured to rotate the stub about an aim point of an ionization laser beam associated with the TOFMS.
  • the sample disk holder may be configured to support the rotation motor.
  • the sample disk holder may be offset relative to an aim point of an ionization laser beam associated with the TOFMS, and additionally, the stub may be rotated about the aim point of the ionization laser beam using a rotation motor to expose a larger area of the sample spot to the ionization laser pulses.
  • the sample stub may include a heating element configured to dry the sample according to a predetermined drying protocol.
  • the stub may be made of electrically insulative material, wherein a high voltage contact plate disposed in the TOFMS station is configured to make electrical contact with the sample disk.
  • a high voltage may be applied under vacuum directly to the sample disk through the high voltage contact plate when the sample disk holder is positioned at the one or more analysis stations.
  • a sample capture and analysis system for analyzing aerosol analyte particles may include a fresh sample disk station configured to receive a fresh disk cartridge having one or more fresh sample disks, a spent sample disk unloader station, an aerosol sample collection station, a rotatable sample disk holder including a stub, the sample disk holder configured to move in at least two directions (9-Z) using one or more of a stepper motor or rotary actuator using a predetermined analysis sequence to engage with each station, and one or more analysis stations.
  • an example sample capture and analysis system may further include a circular gantry plate configured to support each station and engage with the rotatable sample holder, wherein each station may be arranged in a circle.
  • a sample capture and analysis system for analyzing aerosol analyte particles may include a fresh sample disk station configured to receive a fresh disk cartridge having one or more fresh sample disks, a spent sample disk unloader station, an aerosol sample collection station, at least two rotatable sample disk holders, each including a stub.
  • each sample disk holder may include one or more of a stepper motor or rotary actuator and may be configured to move in at least two directions (9-Z) and may be configured to engage with one or more stations according to a predetermined analysis sequence and one or more analysis stations.
  • an example sample capture and analysis system may further include a first semi-circular gantry plate and a second semi-circular gantry plate.
  • the first semi-circular gantry plate may be configured to support a pre-determined first set of stations and engage a first sample holder with each station in the first set of stations, wherein the first set of stations is arranged in a circle.
  • the second semi-circular gantry plate may be configured to support a pre-determined second set of stations and engage a second sample holder with each station in the second set of stations, wherein the second set of stations is arranged in a circle.
  • the first set of stations may include the fresh sample disk loader station, a solvent dispense station, the aerosol sample collection stations, and a camera station.
  • the second set of stations may include the one or more analysis stations and the spent sample disk unloader station.
  • a drying station may be further configured as a disk transfer station and be engaged by both the first sample disk holder and the second sample disk holder.
  • FIG. 1 A shows a perspective view of an example aerosol sample capture and analysis system, according to some implementations.
  • FIG. IB shows a perspective view of sample disk holder for use in the aerosol sample capture and analysis system, according to some implementations.
  • FIG. 1C shows a cross-sectional view of a sample collection station, according to some implementations.
  • FIG. 2A shows a perspective view of an example fresh sample disk cartridge for holding fresh sample disks, according to some implementations.
  • FIG. 2B shows a perspective view of a stack of fresh sample disks in a fresh sample disk cartridge, according to some implementations.
  • FIG. 2C shows a cross-sectional view of a stack of disks in a fresh sample disk cartridge prior to loading a sample disk on a sample disk holder, according to some implementations.
  • FIG. 2D shows a cross-sectional view of a stack of disks in a fresh sample disk cartridge after loading a sample disk on a sample disk holder, according to some implementations.
  • FIG. 2E shows a cross-sectional view of a fresh sample disk cartridge, according to some implementations.
  • FIG. 3 shows a schematic diagram of an example method for aerosol particle sample capture and analysis, according to some implementations.
  • FIG. 4 shows a schematic diagram of an example system resetting method related to method for aerosol particle sample capture and analysis, according to some implementations .
  • FIG. 5 shows raw mass spectra of biological aerosol particles obtained using an example sample capture and analysis system, according to some implementations .
  • FIG. 6 shows processed mass spectrum (bottom) of Bg (Bacillus subtilis var niger) aerosol spores obtained using an example sample capture and analysis system and compared with library Bg spectra (top), according to some implementations .
  • Bg Bacillus subtilis var niger
  • FIG. 7 shows processed mass spectrum (bottom) of Bt (Bacillus thuringiensis al Hakam) aerosol spores obtained using an example sample capture and analysis system and compared with library Bt spectra (top), according to some implementations.
  • Bt Bacillus thuringiensis al Hakam
  • FIG. 9 shows sensitivity of analysis using example aerosol sample capture and analysis system to varying concentrations of Bg particles in air including building dust and laboratory air, according to some implementations.
  • FIG. 10A shows a perspective view of an example solvent dispensing pump and a flow schematic for solvent flow, according to some implementations.
  • FIG. 10B shows an example sequence for dispensing a bolus of solvent on sample disk, according to some implementations.
  • FIGS. 1 1 A and 1 IB show schematic diagrams of example nebulizers for use in an example aerosol sample capture and analysis system, according to some implementations .
  • FIG. 12 shows an example schematic diagram showing data management related to an aerosol sample capture and analysis system, according to some implementations .
  • FIG. 13 shows an example schematic diagram showing software design for an aerosol sample capture and analysis system, according to some implementations.
  • FIG. 14 shows processed mass spectrum of bioaerosol particles including E. coli using an example sample capture and analysis system, according to some implementations .
  • FIG. 15 shows processed mass spectrum of bioaerosol particles including Y. rohdei using an example sample capture and analysis system, according to some implementations .
  • FIG. 16 shows processed mass spectrum of bioaerosol particles including E. coli Bacteriophage MS2 virus using an example sample capture and analysis system, according to some implementations.
  • FIGS. 17A-17B show cross sectional views of a spent sample disk cartridge, according to some implementations.
  • FIG. 17C shows a perspective view of a spent sample disk cartridge component that engages with the stub of a sample disk holder of an example sample capture and analysis system, according to some implementations.
  • FIG. 18 shows a perspective view of an example high-capacity coiled sample disk cartridge, according to some implementations.
  • Figure 19A shows a schematic diagram of a method for collecting and analyzing aerosol analyte particles in air, according to some implementations.
  • FIG. 19B shows a schematic diagram of an example method for dual-stage sample analysis using TOFMS and mass spectra processing, according to some implementations.
  • FIG. 20A shows TOFMS mass spectrum of MALDI matrix coated on a sample disk and analyzed using an example sample capture and analysis system, according to some implementations.
  • FIG. 20B shows TOFMS mass spectrum of aerosolized fentanyl captured from air using an example sample capture and analysis system, according to some implementations.
  • Figure 21 A shows a schematic diagram for identifying an unknown analyte in a sample using an example sample capture and analysis system, according to some implementations.
  • Figure 21 B shows a schematic diagram for generating reference spectra and a runtime machine learning model predicting unknown agents using an example sample capture and analysis system, according to some implementations.
  • FIG. 22 shows a perspective view of an example drying station associated with an aerosol analyte sample capture and analysis system, according to some implementations.
  • FIG. 23 shows a perspective view of a sample disk holder including a stub made of electrically insulative material, according to some implementations.
  • FIG. 24A shows a perspective view of a sample disk holder configured to rotate the sample stub, according to some implementations.
  • FIG. 24B shows a perspective view of an example sample disk, according to some implementations.
  • FIG. 24C shows a perspective view of another example sample disk, according to some implementations.
  • FIG. 25A shows a perspective view of an example aerosol sample capture and analysis system, according to some implementations.
  • FIG. 25B shows a schematic top view of an arrangement of one or more stations in an example aerosol sample capture and analysis system, according to some implementations.
  • FIG. 25C shows a schematic top view of an arrangement of one or more stations in an example aerosol sample capture and analysis system, according to some implementations.
  • aerosol generally means a suspension of particles dispersed in air or gas.
  • sample disk or “sample substrate” means a solid (typically metal) surface upon which a sample may be deposited.
  • “Real-time” or “near real-time” analysis of aerosols generally means analytical methods and devices that identify the aerosol analyte within a matter of several minutes (e.g., less than about five minutes) after the aerosol sample to be analyzed is collected.
  • the terms “a” or “an” are used to include one or more than one, and the term “or” is used to refer to a nonexclusive “or” unless otherwise indicated.
  • FIG. 1 A shows a perspective view of an example autonomous aerosol sample capture and analysis system, according to some implementations.
  • FIG. IB shows a perspective view of sample disk holder for use in the autonomous system, according to some implementations.
  • An example autonomous sample capture and analysis system 100 may include a fresh sample disk or substrate loader station 101, a spent sample disk or substrate loader station 102, an aerosol sample collection station 103, a camera station 104, a liquid chemical dispensing station 105 and a TOFMS analysis station 106 disposed on a suitable frame 107.
  • a sample disk holder 108 is disposed in frame 107 and is configured to be movably disposed under each station.
  • Sample disk holder 108 is configured to move horizontally (X-Y axis) using linear actuator and stepper motor 1 10. Sample disk holder 108 may also be configured to move vertically (Z direction) using stepper motor and linear actuator 111. That is, sample disk holder 108 may be configured to autonomously (robotically) move between the stations (horizontal movement) and engage with each station using vertical movements.
  • An example disk 112 may be 6 mm in diameter and may be made of one or more of nickel or nickel alloys. The thickness of disk 112 may be between about 0.05 in. and about 0.01 in.
  • Dispensing station 105 is configured to dispense between about 0.5
  • FIG. 10A shows a perspective view of an example solvent dispensing pump and a flow schematic for solvent flow, according to some implementations.
  • Another example dispensing pump is a peristaltic pump 220 (Insetek, Inc., CA) as shown in FIG. 10A.
  • Pump 220 may be suitable for use with chemical-resistant tubing with an internal diameter of 1 mm or less, and an outer diameter of 1mm or less.
  • Sample loader 108 in addition to holding a sample disk 1 12, also holds an adsorbent card or pad.
  • Adsorbent card 221 is disposed adjacent to the sample holder and is used to adsorb liquid dispensed when the tubing is purged.
  • the adsorbent card may include a woven or non-woven hydrophilic porous sheet of about 0.1 mm to about 1 mm thickness.
  • the card material may include one or more of a paper fiber, nylon, or polytetrafluoroethylene (PTFE) woven sheet.
  • PTFE polytetrafluoroethylene
  • An example chemical-resistant tubing type is C-FLEX tubing (Cole-Palmer Vernon Hills, IL).
  • the chemical liquid may include TFA, acetonitrile, methanol, ethanol, and water.
  • the liquid may include about 70 vol.-% acetonitrile, about 15 vol.-% TFA, with the remaining being water.
  • the liquid may include about 70 vol.-% methanol, about 15 vol.-% TFA, with the remaining being water.
  • the volume of liquid dispensed is between about 0.5 pl and about 2 pl to obtain a sample spot of about 1 mm diameter (with a nozzle 165 diameter of about 0.7 mm) on the sample disk. At higher ambient temperatures of greater than about 35 °C, the dispensing volume may need to be higher to compensate for evaporation losses of the solvent.
  • the dispensing volume and nozzle diameter may be varied to achieve a spot of between about 0.5 mm and about 1.5 mm in spot size.
  • system 100 or a portion thereof may be disposed in a space with temperature and humidity control.
  • a preferred enclosure for system 100 is a sealed, temperature-controlled case.
  • One or more desiccants or adsorbents loaded into a suitable adsorber component may be provided within the case to remove water and solvent vapors to ensure that system 100 is warm and dry, and sample drying preferably occurs in a warm and dry ambient condition.
  • “warm” means between about 30 °C and about 45 °C and “dry” means below about 25% relative humidity.
  • the dispense tube tip 240 includes one or more of stainless steel, or a solvent-resistant, acid-resistant polymer such as PTFE.
  • FIG. 10B shows an example sequence 250 for dispensing a bolus of solvent on sample disk 112, according to some implementations.
  • sample adsorbent card 221 is positioned under the dispense tube in step 251.
  • pump 220 is activated for sufficient duration to completely purge any fluid disposed in the dispense tube downstream of pump 220.
  • sample disk holder 108 with disk 112 is position (which may be configured to robotically move between stations as previously described) under dispense tube tip 240, and in step 254, liquid is dispensed onto the sample disk 112. Pump speed and operation may be controlled by the system controller (not shown).
  • disk 112 may be precoated with MALDI chemical including alpha-cyano-4-hydroxycinnamic acid dissolved in a solvent.
  • Solvents may include acetonitrile, water, methanol, ethanol, and acetone.
  • system 100 may include a MALDI matrix coating station to coat disk 112.
  • Fresh disk 112 may be coated prior to being loaded into a fresh sample disk cartridge 200; that is, prior to being loaded into the system 100.
  • MALDI matrix solution may be added from a reservoir to the sample after it has been deposited on a disk.
  • the reservoir may need to be heated and stirred using a magnetic stirring to keep the matrix in solution.
  • system 100 may also include a homing sensor to align the movable sample disk holder 108 with each station.
  • Z-axis homing sensor 118 is shown in FIG. IB.
  • Stepper motors (with linear actuators) 110 (X-Y axis) and 111 (Z-axis) may be controlled using a microcontroller.
  • Stepper motor and linear actuator 111 may be mounted on X-Y axis carriage 109.
  • stub 116 may be isolated from the TOFMS using electrical insulator element 117.
  • Aerosol collection station 103 may include a * ” O.D. SS 304 or SS 316 alloy tubing 113.
  • a flexible tubing may be removably connected to inlet tubing 113 at end 115 and positioned in the area to be sampled.
  • an ambient air sample may be drawn into inlet tubing 113 using an aerosol pump (not shown) that is connected to outlet tube or fitting 114 in station 103.
  • the flexible tubing may be connected to a ventilation duct to collect a sample of the air flowing in the duct.
  • FIG. 1C shows a cross-sectional view of a sample collection station, according to some implementations.
  • the end of inlet tubing 113 that is disposed opposite to end 115 may be configured to feed the aerosol to the impactor nozzle 165 as shown in FIG. 1C.
  • Nozzle 165 may be characterized by a hole diameter at the nozzle tip 167 of between about 0.35 mm and about 1 mm.
  • Spacing 166 disposed between the nozzle tip 167 and sample disk 112 may be about the size of the nozzle diameter; that is, between about 0.35 mm and about 1 mm.
  • tubing 113 and impactor nozzle 165 may be heated to reduce the relative humidity of sampled air, in particular, in instances where the air sample collected by ambient conditions characterized by high humidity.
  • FIG. 2A shows a perspective view of an example cartridge for holding fresh sample disks, according to some implementations.
  • FIG. 2B shows a perspective view of a stack of sample disks in a cartridge, according to some implementations.
  • FIG. 2C shows a cross-sectional view of a stack of disks in a cartridge prior to loading a sample disk in a sample disk holder, according to some implementations.
  • FIG. 2D shows a cross-sectional view of a stack of disks in a cartridge after loading a sample disk in a sample disk holder, according to some implementations.
  • FIG. 2E shows a cross-sectional view of a cartridge, according to some implementations.
  • loader station 101 may include a sample disk loader cartridge or magazine 200.
  • the bottom end of cartridge 200 is configured to mechanically engage with stub 116 of sample disk holder 108.
  • Stub 116 may be made of one or more of aluminum or aluminum alloys.
  • stub 116 When stub 116 is inserted into sample disk cartridge 200 actuated by stepper motor and Z-axis linear actuator, it causes flexible wings 201 (or loader mechanism) to flex outward and forces the release of a sample disk 112 from the stack of sample disks in cartridge 200 onto the stub 116.
  • FIG. 2C the surface of each disk 112 is separated from another disk on account of its geometrically capped shape 204 of each disk 112.
  • the metal sample disk is held in place on the stub using a suitable magnet 202 mounted on the end of stub 116. Withdrawing stub 116 from cartridge 200 causes the flexible arms or wings 201 to return inward, and tabs (or hooks) 203 engage with the next available disk (FIG. 2D). The next available sample disk is now in position to engage with stub 116 for sampling of a subsequent sample. In sample disk cartridge 200, the next available disk 112 is held in place using tabs or hooks 203. The sample disks are positioned in place using loader mechanism retainer 205, push road 206, and weight 207.
  • camera station 104 may include a microscope camera and a digital camera removably affixed to mount bolt 104’ . These cameras may be used to review the image of the disk between stations, for example, to check whether a fresh disk 1 12 has been properly loaded from the loader 200 (whether a fresh disk has been extracted at loader station 101), whether the sample deposited at sample capture station 103 is of a desired spot size, and whether the sample has sufficiently dried.
  • System 100 may also include a data logging system to log the position and sample details (e.g., camera image) collected during the course of the analysis.
  • the running of the suction pump may be initiated when the particle counts in ambient air, as measured using a suitable counter, exceed a predetermined threshold.
  • Other types of cameras that may be used at camera station 104 include fluorescence microscope cameras, hyperspectral imaging cameras, and thermal imaging cameras.
  • hyperspectral and thermal imaging cameras may be able to more accurately measure the mass of sample collected and the degree to which the sample has been dried.
  • Stub 116 may form a vacuum tight seal with the vacuum chamber of a TOF-MS using O-ring seal 119.
  • sample disk holder 108 is configured to return the used or spent disk to spent disk cartridge at station 102.
  • Stub 116 with a spent disk held in place using magnet is raised to engage the disk with the flexible arms or wings 201 of cartridge at station 102.
  • Finite element analysis shows that about 0.1 lb. outward force exerted on the flexible arms by the stub produces a radial displacement of about 0.015 in., which is sufficient to release tabs (or hooks) 203 and move the spent disk into the spent sample disk cartridge at station 102.
  • system 100 may also include a data processing system for acquiring and processing data output from the analysis station.
  • Data processing may include the steps of filter/smoothing, baseline subtraction, signal to noise ratio estimation, peak detection, feature extraction, detection and classification and reporting including reporting via a user interface. Detection and classification may be achieved by comparing with reference spectra to identify the composition of the aerosol particles in the sample (e.g., biohazard particles that include, but are not limited to, ricin).
  • Machine learning (ML) techniques for analyzing collected spectral data obtained using a machine learning engine offers a significant improvement to manual data processing for analyte identification, which is slow and labor intensive.
  • Machine learning is generally a subset of artificial intelligence and include algorithms whose performance improve with data analysis over time.
  • Supervised machine learning methods may be used. Supervised learning includes the task of learning a function that maps an input to an output based on example input-output pairs. It infers a function from labeled training data consisting of a set of training examples.
  • Machine learning also includes deep learning methodologies which are unsupervised learning methods that can identify signatures in complex data sets without the need to a priori identify specific features. Unsupervised machine learning methods and semisupervised (hybrid methods between supervised and unsupervised learning) may also be used.
  • Unsupervised learning methods may include a type learning that helps find previously unknown patterns in data set without pre-existing labels.
  • Two example methods used in unsupervised learning are principal component and cluster analysis.
  • Cluster analysis is used in unsupervised learning to group, or segment, datasets with shared attributes in order to extrapolate algorithmic relationships.
  • Cluster analysis is a branch of machine learning that groups the data that has not been labelled, classified or categorized.
  • Cluster analysis identifies commonalities in the data and reacts based on the presence or absence of such commonalities in each new piece of data. This approach helps detect anomalous data points.
  • Unsupervised learning methods may be used for anomaly detection, which can be helpful in identifying previously unknown hazards.
  • air samples may be analyzed at periodic intervals to measure the composition of particles in air and to identify the properties of the particles (e.g., size, shape, fluorescence) and spectra associated with particles to get a baseline data information of particles in “normal” ambient air.
  • Particles in ambient air after an event such as the release of biological threat agents into the atmosphere would provide particle property data and spectral data that deviate from baseline data and would highlight an anomaly (as evidenced by anomalous spectra) and provide an opportunity to take necessary remedial steps to mitigate the threat.
  • the compiled spectral data may be compared with a training data set including a knowledge base of known biological matter spectra to predict particle composition.
  • An example sample preparation and analysis system may be in data communication with the machine learning engine to allow for updating the training data set knowledge based and improving the prediction of composition over time.
  • Biological matter mass spectra cover a range that is about three orders of magnitude greater than chemical mass spectra, significantly complicating the application of automated techniques.
  • environmental contaminants can reduce signal strength by competing with the target during the ionization process (competitive ionization), a introduce signature components (clutter) that must be deconvolved with the target signature.
  • Current automated methods are mostly limited to searching for very pure targets in samples with no environmental clutter.
  • the disclosed example methods eliminate competitive ionization by physically separating target analyte from clutter and eliminates ambiguities in the signature (each event is assumed to be an either target or clutter). Additional details on integrating machine learning methods for analysis of spectral data related to aerosol samples are disclosed in commonly owned U.S. Prov. Pat. Appl. No. 62/868,906 titled “Methods and Systems for Detection of Aerosol Particles Without Using Complex Organic MALDI Matrices,” which is incorporated by reference herein in its entirety.
  • FIG. 12 shows an example schematic diagram showing data management related to an autonomous aerosol sample capture and analysis system, according to some implementations.
  • Data output from the analysis station may be processed using an external data processing station 1201 in data management system 1200 and data may be transferred from system 100 to the processing station via wired (ethernet, LAN) or wireless bidirectional communication.
  • Data in various formats such as raw data, filtered data, data logs, alarms and operating parameters may be stored in one or more of local storage server 1202 or remote or cloud storage server 1203.
  • the servers are configured to be in bidirectional secure communication with the data processing station 1201.
  • a mobile application software 1204 (“app”) may also be configured to monitor the operating status of example system 100, initiate data processing, and view and report output or results from data processing station 1201 .
  • a mobile application software or “app” is a computer program configured to run on a mobile device such as a smart phone, tablet or watch.
  • An app includes a front-end component or user interface (“UI”) and is designed to provide the user with an easy-to-use and friendly interface.
  • the front end communicates with a back-end component which facilitates data routing, security, authentication, authorization, working off-line, and service orchestration.
  • An app may also communicate with one or more intermediate or middle components including, but not limited to, mobile app servers, message queuing, enterprise service bus (“ESB”) and other service-oriented architecture (“SOA”) infrastructure components.
  • ESD enterprise service bus
  • SOA service-oriented architecture
  • Data synchronization between the mobile device and a database or cloud and offline (without internet connection) capabilities are key to the seamless functioning of successful mobile apps.
  • Providers of database and cloud services such as Couchbase Mobile (Couchbase), Azure Mobile Services (Microsoft), Cognito (Amazon), Firebase (Google) offer synchronization and offline capabilities with their mobile offerings.
  • the app should preferably provide for secure data access communication with synchronized and decentralized storage, transmission and storage using features such as address authentication, data at rest, which relates to whether the app supports file system encryption and data-level encryption, data in motion, and read/write access that defines what data may be accessed and changed/modified by users.
  • Databases may be relational (SQL databases such as Oracle, mySQL) or NoSQL (e.g., MongoDB, CouchDB).
  • SQL databases such as Oracle, mySQL
  • NoSQL e.g., MongoDB, CouchDB
  • the app should preferably incorporate a mechanism for resolving those conflicts.
  • the conflict resolution mechanism may allow resolution automatically, on the device, in the cloud, or could be manually initiated.
  • FIG. 13 shows an example schematic diagram showing software design 1300 for system 100, according to some implementations.
  • the operation of components 1301 in system 100 may be controlled by controller 1302.
  • a mobile application software (“app”) may be configured to monitor the operating status of example system 100, initiate data processing, and view and report output or results.
  • System 100 may include a plurality of loader stations 101.
  • a first loader station 101 may include disks 112 coated with MALDI matrix and a second loader station 101 may include uncoated disks.
  • Example system 100 may also include a loader station 101 including a stack of alternating coated and uncoated disks.
  • the uncoated sample disk may be pulled from the fresh disk stack (cartridge or magazine) 200 and moved to the spent disk stack to cartridge at station 102, thereby allowing the robotically operated sample holder 108 to access an uncoated sample disk 112 in the fresh disk stack.
  • system 100 may include a plurality of loader and unloader stations that reside on a carousel. As one sample disk cartridge is exhausted, the carousel may rotate to present a fresh sample disk cartridge containing a fresh stack of coated or uncoated sample disks. Alternatively, two carousels may provide one for fresh sample disks and one for spend sample disks.
  • Machine learning techniques may also be used for the analysis of images produced by camera station 104. For example, a neural network and one or mode operational models may be trained to determine if a sample disk 112 has been properly loaded at station 101, or properly unloaded at station 102, or assess if the sample is dry or requires drying.
  • pre-depositing the disks with a MALDI matrix may not be needed.
  • chemical aerosol particles include, but are not limited to ricin, fentanyl, and carfentanyl. These chemical agents are relatively easy to produce and may be more readily available that biological agents such as anthrax and have been recently used as a chemical weapon.
  • the aerosol particles may be directly deposited on the sample disk or substrate that is not precoated with a MALDI matrix, dried if necessary, and analyzed using a TOF-MS that is operated as Laser desorption/ionization mass spectrometer (LDLTOFMS).
  • LDLTOFMS Laser Desorption/Ionization-Time of Flight Mass Spectrometry
  • MALDI TOFMS would be preferable for large organic compounds such as polymers.
  • FIG. 3 shows a schematic diagram of an example autonomous method for aerosol particle sample capture and analysis, according to some implementations.
  • sample disk holder 108 having a fresh disk 112 pre-coated with MALDI matrix is moved from TOFMS station 106 to station 103 for impacting the disk with the aerosol sample.
  • the gate valve of the TOFMS station Prior to moving the sample from station 106, the gate valve of the TOFMS station is closed.
  • the aerosol pump is turned-on for a predetermined time to draw ambient air into tubing 113 and to impact particles on coated disk 112.
  • the pump is turned off and the sample disk holder 108 is moved to station 105 in step 303 for treating the deposited sample with chemicals in step 304.
  • step 305 the sample disk holder having the disk is then moved to camera station 104 for examination using one or more of a microscope camera or imaging using a digital camera.
  • step 306 drying of the sample is initiated.
  • the sample is periodically monitored by taking images of the sample in station 104 (step 307) and drying is continued (step 308), for example by heating the sample using a heater, if images show that the sample is still wet.
  • step 308 the sample holder is moved to station 106 for TOFMS analysis.
  • step 309 the sample stub 116 is sealed with the vacuum chamber of the TOFMS and the chamber is pumped down in step 310. If the sample heater is present and has been turned on, the heater is turned off.
  • the ionization laser of the TOFMS is triggered in step 311, spectra is collected in step 312 and analyzed in step 313.
  • FIG. 4 shows a schematic diagram of an example system resetting method related to autonomous method for aerosol particle sample capture and analysis, according to some implementations.
  • the reset process 400 includes the steps of moving the spent disk to spent disk cartridge at station 102 (step 401) and inserting the disk into cartridge station 102 (step 402), moving the stub to station 104 for imaging (step 403) to check if the spent disk has been removed from the stub in step 405. If the spent disk is still present on the stub 116, a maintenance alert is raised in step 406.
  • sample disk holder 108 is moved to fresh disk cartridge station 101 in step 407, and stub 116 is inserted into the cartridge at station 101 in step 408.
  • the stub is moved to station 104 for imaging in step 409 and images are collected in step 410. If images show that the disk is present on stub 116 in step 411, sample disk holder 108 is moved to the aerosol sampling station 103 for sample collection. If the disk is not present, a maintenance alert is raised. Machine learning methods may be used to automate the review of images taken during the reset process.
  • the sample disk holder is moved to station 106 (the MS station) in step 412 so that the fresh sample disk 112 may be stored under vacuum. The gate valve on the TOFMS system may then be opened, exposing the fresh disk to vacuum.
  • aerosol particles may be collected onto a substrate by impaction as disclosed in U.S. Pat. Pub. No. 2003/0020011, which is incorporated by reference herein in its entirety.
  • a virtual impactor, or multiple stages of virtual impaction may be incorporated into sample collection station 103 to concentrate the aerosol particles prior to impaction on sample disk 112 as described in U.S. Patent No. 7,799,567 “AIR SAMPLER BASED ON VIRTUAL IMPACTION AND ACTUAL IMPACTION,” which is incorporated by reference herein in its entirety.
  • a MALDI matrix solution may then be added to the sample.
  • the MALDI matrix solution may include alpha-cyano-4-hydroxycinnamic acid dissolved in a solvent.
  • Solvents may include acetonitrile, methanol, water, ethanol, and acetone.
  • the MALDI matrix solution is spotted on to the analyte on a MALDI plate to yield a uniform homogenous layer of MALDI matrix material on the analyte.
  • the solvents vaporize, leaving only the recrystallized matrix with the analyte spread through the matrix crystals.
  • the coated plate or substrate dried and analyzed in a TOFMS.
  • “Whole cell” analysis can provide identification in less than about 5 min.
  • the system may further include one or more of an aerodynamic particle sizer, an optical particle counter, or a device that provides for fluorescence or depolarization measurement on each particle.
  • An example aerosol particle sizer is manufactured by Air Techniques International, Inc. (Maryland). Measurement techniques such as fluorescence and depolarization assist in distinguishing threat aerosol particles from normal ambient aerosol particles, as making this determination based solely on size and counts per unit of time is unreliable.
  • This optical detection component is preferentially added in parallel or upstream of the impaction collection step. Information about the size of particles associated with the threat aerosol particles may be used to optimize the collection of these particles.
  • particles with mean particle diameter of about Ip require a higher velocity to drive impaction than particles with mean particle diameter of about 3p.
  • the mass of the about 3p diameter particles is about 27 times greater than that of the about I particles, assuming particle densities are comparable. Since inertial separation is proportional to particle mass and particle velocity, the operation of the pump used in aerosol collection station 103 may be adjusted to cause a higher air flow velocity through the nozzle for the 1 p particles or slowed down to decrease air flow velocity if the particles to be collected were larger in size (e.g., about 3p).
  • the optical detector may be utilized to optimize the collection efficiency of aerosol particles based on the mean particle diameter of the threat aerosol particles.
  • the number density of threat particles may be used to determine the duration of the sampling period. For example, if the threat aerosol is a dense aerosol with a high particle loading of threat particles, a shorter sampling period may be sufficient to obtain a good sample; when the threat aerosol concentration is dilute, a longer sampling period would be required.
  • the aerosol particles may be collected into a liquid by impaction or impingement, for example, using an impactor device as disclosed below.
  • the sample may then be subjected to enzyme or hot acid treatment for several minutes. Digestion may be done at about 140 °C for about 15 min. Hot Acid cleaves proteins at aspartic acid (Asp) residues creating highly specific peptides. Chemical digestion of the protein signature molecules will provide a proteomic peptide map in about 15 minutes. Individual peptides may be micro-sequenced to identify amino acid sequences of known biomarkers.
  • an activity assay may unambiguously determine that “live” toxin is present in the sample. This assay may take between about 1 h and 2 h to complete. MALDI matrix solution is then added to the treated sample. The sample may be dried and analyzed in a TOFMS. [0109] Autonomous sample analysis is not limited to mass spectrometry and optical imaging.
  • fluorescence microscopy In another example method for analyzing a sample collected on the sample disk, fluorescence microscopy, RAMAN spectroscopy, surface enhanced RAMAN spectroscopy, scanning electron microscopy, and other surface-based analyses may be employed at other discrete stations in system 100 and accessible to the sample holder 108 and the sample handling robotic carriage 109.
  • a MALDI matrix chemical in a solvent may be deposited on a substrate that includes, but is not limited to, disks made of a suitable metal, metal alloys and other high electrical conductivity materials.
  • An example sample disk 1 12 may be 6 mm in diameter and may be held in a sample disk holder 108.
  • the MALDI matrix chemical may include alpha-Cyano-4- hydroxycinnamic acid dissolved in a solvent.
  • Solvents may include acetonitrile, water, ethanol, and acetone.
  • the matrix chemical may be substantially dried to form a thin film on the disk.
  • the pre-coated disk may then be placed under a spotting nozzle that is capable of depositing sample aerosol particles onto the disk in a sampling period of less than about 1 min.
  • the sample aerosol may include particles in ambient air.
  • Other chemicals such as tri-fluoro-acetic acid (TFA), alcohol and water or mixtures thereof may be added to the deposited sample.
  • TFA tri-fluoro-acetic acid
  • alcohol alcohol
  • water water
  • Water enables hydrophilic proteins to dissolve, and acetonitrile or organic solvents enable the hydrophobic proteins and lipids to dissolve.
  • one or more images of the sample spot may be captured using a suitable camera. Images of the sample spot may be collected and analyzed after the chemical treatment to monitor the drying process and to determine if the sample has substantially dried. High magnification imaging may be used to provide information about the morphology of the collected particles. Florescence imaging or thermal imaging may be used to provide additional information about the location of material on the disk or the dryness of the sample.
  • FIG. 22 shows a perspective view 2200 of an example drying station associated with an aerosol analyte sample capture and analysis system, according to some implementations.
  • sample disk holder 2205 after receiving an aerosol sample on sample disk 2202 supported on stub 2203 at aerosol sample collection station (reference numeral 103, with reference to FIG. 1 A), and/or after treating the sample with a solvent or other liquid at the liquid chemical dispensing station (reference numeral 105, with reference to FIG.
  • Drying station 2206 may include a conduction oven 2204 including one or more heating elements 2201 that may be energized to dry the sample in a controlled manner in accordance with a predetermined drying protocol.
  • An example drying protocol may include varying one or more of drying time or degree of drying (for example, partially dry, completely dry, 50% dry as other non-limiting drying levels).
  • the degree of drying may be determined by periodically examining the sample at camera station (reference numeral 104, with reference to FIG. 1 A) during the drying process.
  • an example drying time may be about 2 min.
  • the sample disposed on sample disk 2202 may be heated by one or more of convection, induction, or radiation.
  • a heating element may be integrated into stub 2203 (not shown for simplicity) to heat the stub, and subsequently dry the sample.
  • a heated stub may reduce the variability of the drying process, decrease pump-down time (for example, under vacuum) or may reduce the possibility of condensation on the sample disk when drawing in humid air that is warmer than the stub to dry the sample.
  • warm air used for drying may be dehumidified by flowing it through a bed of desiccant material to further enhance the drying process.
  • An infrared emitter element which emits wavelengths that are strongly absorbed by water may also be used, for example, in the mid-infrared region.
  • the sample generated using the example methods and disposed on the disk results in a small, round, substantially dry aerosol spot or deposit, at or near the center of a sample disk.
  • the disk may be precoated with a MALDI matrix chemical solution prior to sample deposition.
  • a MALDI matrix chemical solution may be mixed with the deposited sample prior to drying.
  • System 100 may also include a dedicated drying station in which the sample is dried under vacuum.
  • One or more of the roughing pump or the turbopump associated with the TOFMS in station 106 may be fluidly connected to the drying station.
  • the sample may then be analyzed using a mass spectrometer. For example, between about 2 and about 200 discrete mass peaks (spectra) characteristic of the sample may be generated by exposing the sample to at least one laser ionization pulse. The number of laser ionization pulses may be between about 1 and about 20.
  • Pre-deposition of the MALDI matrix as a thin film prior to adding the aerosol sample avoids the need to nebulize the matrix and/or the aerosol prior to depositing on the disk and provides for aerosol particles that substantially uniformly MALDI- matrix coated aerosol particles.
  • the example methods also improve adhesion of particles, potentially due to the addition of surface roughness or surface stickiness, and thus, avoid or minimize loss of aerosol particles when a high velocity stream impinges on a collection surface creating a tendency of the particles to “bounce-off” the substrate after impaction.
  • An optimal air flow velocity to minimize bounce-off while maximizing the number of particles that impact the surface is about 75 m/s but may vary between about 50 m/s and about 150 m/s, depending on the properties of the impaction surface, the orifice diameter, and particle cut size.
  • the particle cut size is the diameter of particle for which half of the particles of this size are collected by the impactor and half are transmitted through the impactor.
  • the example method described above may be configured to be autonomous.
  • an example aerosol sampling robotic system 100 may be used to perform the steps of:
  • the example method described above may further include capturing a digital image of the disk by moving the sample holder including the disk to camera station 104 between two consecutive steps to examine if the sample holder has extracted a disk from loader station 101 in step (a), whether sample has been deposited in step (b), whether drying is substantially complete in step (d), and the like.
  • Camera station 104 may include one or more light sources (e.g., light emitting diodes or LEDs) to illuminate the surface of the disk.
  • the image of the surface of a disk having a liquid droplet including a sample or treatment solution on it would typically have a glassy smooth surface capable of reflecting light, for example, via specular reflection, which may be considered to be a mirror-like reflection of light from the surface.
  • the image may therefore show the discrete LEDs illuminating the surface.
  • the disk when the disk is substantially dry, that is when effective drying is achieved, the surface tends to scatter light, and the image does not show the discrete LEDs illuminating the surface.
  • Examination of digital images collected during the steps may also be automated by comparing the captured image after each step with a library of standard or baseline digital images to decide if the sample disk holder 108 including the disk should be moved to the next step or whether the disk should be discarded at spent disk cartridge 102.
  • the samples on the post-analysis disks may also be extracted and analyzed using PCR (polymerase chain reaction) and other biomolecular or microbiological techniques to confirm MALDI TOFMS results if needed.
  • the initiating the analysis process step (f) may include the steps of evacuating the MS station 106 with the sample disk holder 108 sealed to it to less than about 10‘ 5 torr, and preferably less than about 5x10‘ 6 torr, applying a voltage to the electrically conductive disk to generate a strong electric field in the region near the surface of the disk, focusing a laser pulse to vaporize the MALDI matrix and analytes and generating ions, and accelerating the ions to the detector by controlling the direction of the electrical field.
  • the example method may further include the step of positioning an uncoated disk under a third nozzle for depositing the MALDI matrix solution and substantially drying the matrix.
  • Sample aerosol particles may not be limited to collection by impaction at station 103.
  • Sample aerosol particles may be also collected using a suitable impaction or liquid impingement device that is used for collecting particulates (e.g., sample aerosol particles) dispersed in gaseous streams (e.g., ambient air). Aerosol particles usually concentrate in a small volume of water or a liquid and may be spotted on a disk using a dispensing station similar to that of station 105.
  • An impact device forces a large volume of air into the device, and concentrates any particulates collected to provide a high-quality representative sample.
  • 6,267,0166 which is incorporated by reference herein in its entirety, discloses a rotary collector including a combined impact collector and fan, which draws in air or other gaseous fluid in which particulates are entrained into a cavity, and then separating the particulates from the gaseous fluid by providing a rotating surface that impacts the particulates.
  • the particulates also impact on other surfaces within the cavity, including its inner surface, and are washed from these surfaces, which are wetted with water or other liquid injected into the cavity.
  • the water or other liquid is either continuously or intermittently injected into the cavity to wash the particulates from the impeller vanes and other surfaces on which they have impacted.
  • the particulates are carried by the liquid through a threaded drain port, into a receiver that includes an exhaust port for the air or gaseous fluid.
  • a pump recirculates the liquid from the receiver through a conduit that sprays the liquid into the cavity through the inlet port.
  • the particulates collected in the receiver provide a specimen that can be analyzed to detect or identify the particulates that were entrained in the air or other gaseous fluid.
  • U.S. Pat. No. 6,695,146 discloses a virtual impactor to separate a flow of fluid into a major flow and a minor flow, such that the minor flow contains a higher concentration of particulates of a desired size.
  • the minor flow is directed toward an archival surface, causing the particulates to impact against and be deposited on the archival surface.
  • the archival surface and the virtual impactor are moved relative to one another such that particulates collected at different times are deposited as spaced-apart spots on different portions of the archival surface.
  • the particulates are stored on the archival surface until analysis of the particulates is required.
  • the archival surface can be coated with a material that enhances the deposition and retention of the particulates and can further be coated with materials that sustain the life of biological organism particulates deposited on the archival surface.
  • the disclosures of U.S. Pat. No. 6,695,146, U.S. Pat. No. 6,290,065, U.S. Pat. No. 6,062,392, and U.S. Pat. No. 6,363,800 are incorporated by reference herein in each of their entirety.
  • U.S. Pat. No. 7,799,567 discloses a method for removing particles from a fluid stream, such as air, and depositing the particles on one or more collection surfaces.
  • a pre-filter is employed to reject oversized particles and contaminants, such as rain and insects.
  • a concentrator is employed to increase a concentration of particles larger than a pre-defined particle size in at least a portion of the fluid, producing a concentrated volume of fluid.
  • the concentrator and pre-filter can, for example, be virtual impactors.
  • U.S. Pat. No. 7,759,123 discloses a method and apparatus for removing concentrated spots of collected particulates from an impact collection surface and transferring those particulates into a container suitable for preparing a liquid sample. A jet of fluid is utilized to remove and transfer the particulates. If a liquid jet is employed, the quantity of liquid is minimized to avoid unnecessarily diluting the sample.
  • the aerosol particles to be analyzed need not be limited to particles found in ambient air.
  • analyte aerosol could include exhaled breath particles (EBP) found in exhaled air of humans or animals.
  • EBP exhaled breath particles
  • the volume of air exhaled during breathing in healthy adults is typically between 1-2 liters, which includes a normal tidal volume of about 0.5 liters.
  • Humans produce exhaled breath particles (EBPs) during various breath activities, such as normal breathing, coughing, talking, and sneezing.
  • EBP concentrations from mechanically ventilated patients during normal breathing may be about 0.4 to about 2000 particles/breath or 0.001 to 5 particles/mL.
  • the size of the EBP’s may be below 5 micrometers, and 80% of them may range from 0.3 to 1.0 micrometers. Exhaled particle size distribution has also been reported to fall between 0.3 and 2.0 micrometers.
  • the mean particle sizes of EBPs may be less than 1 micrometer during normal breathing, and 1 to 125 micrometers during coughing.
  • the sample collection subsystem may include a sample extraction component configured to receive an individual’s face for extracting one or more of breath aerosol (EBA) particles or lipids biomarker characteristic of Mycobacterium tuberculosis (Mtb) expelled from the individual during predetermined breath maneuvers into a flow of air fed into the extraction component, a sample capture component fluidly connected to the sample extraction component by an interface tubing and configured to separate and collect EBA particles and lipids from exhaled breath and air as a collected sample.
  • EBA breath aerosol
  • Mtb Mycobacterium tuberculosis
  • One or more chilling devices may be configured to be in thermal communication with the walls of at least one of the interface tubing.
  • An example of a thermoelectric cooling device is manufactured by Marlow Industries (Dallas, TX).
  • the sample capture component and a sample analysis subsystem are fluidly connected.
  • the collected sample may be concentrated on a sample plate using a station that is similar to station 105 used for dispensing chemicals.
  • the volume of the collected sample may be less than about 1 ml.
  • the volume of the collected sample may be less than 100 pL and may be less than 2 pL.
  • Methods and devices, that include, but are not limited to membrane-based separation or evaporation may be used to concentrate the liquid sample from milliliter volumes to microliter volumes.
  • Microbial cell walls or spore coats may be opened or “lysed” to improve analysis of microbes using example system 100 by exposing more of the microbe’s characteristic cell contents to MALDI analysis.
  • U.S. Pat. No. 5,989,824 titled “APPARATUS AND METHOD FOR LYSING BACTERIAL SPOES TO FACILITATE THEIR IDENTIFCATION” discloses use of a plasma or electric discharge to break open cells or spores resting on a metal substrate and is hereby incorporated by reference herein in its entirety. Lysing may also be achieved using chemical, focused acoustic, and focused electromagnetic treatments.
  • the methods previously described may be modified when the sample includes both biological and chemical particles.
  • a bare disk or substrate (without any MALDI matrix coating) may be positioned under the spot sampler and an aerosol sample collected using any one of the previously disclosed collection systems 100 may be deposited on the disk.
  • the disk may then be transported to an LDI-TOFMS instrument for analysis of the chemical composition and identification.
  • the same sample disk may then be transported to a station in the example systems 100 where chemicals are added to the sample spot.
  • the chemical mixture may include alpha-Cyano-4-hydroxycinnamic acid, methanol, TFA, and water.
  • the sample may be dried and analyzed in a MALDLTOFMS for biological composition and identification. Subsequently, both chemical and biological threat aerosol particles may be rapidly identified from a single sample.
  • fresh sample disks may be stored in two separate locations, namely, one location at which disks coated with MALDI matrix are stored, and another location at which disks that are uncoated are stored.
  • alternating sample disks could be coated and if a coated sample disk is needed when an uncoated sample disk is at the bottom of the fresh sample disk stack, the uncoated sample disk could be pulled from the fresh disk stack moved to the spent disk stack, thereby allowing the robot to access an uncoated sample disk in the fresh disk stack. These movements may be accomplished in a matter of several seconds.
  • air samples may be collected at predetermined time intervals and analyzed using the example methods disclosed above to generate a historical data set (training data set) of background/baseline information. Analysis may be improved by time using machine learning algorithms. Variations in background information may be modeled to map out normal behavior of the atmosphere in a protected area. When a release of biological, biochemical, or chemical aerosol particles is suspected, sampling of air using the example methods described above will result in information that deviates from historical background information. The first signature of the presence of such a threat will be a sharp deviation from the normal background. At this stage, algorithmic decisions may be made as to the composition of each individual particle. Remedial actions can therefore be taken quickly to protect human life and to prevent loss of life. For example, a building’s heating, ventilation, and air conditioning (HVAC) system may be shut down to limit the spread of the aerosol and the fire alarm can be activated to evacuate the building.
  • HVAC heating, ventilation, and air conditioning
  • Liquid samples may also include chemical liquids that are at the beginning of the semiconductor fabrication process or front-end-of-line (FEOL) and chemicals that are used at the back-end-of- line (BEOL) stage of the fabrication process.
  • Ultrapure water in the semiconductor industry refers to water has been treated to the remove almost all contaminants including biological (for example, bacterial matter), metals and metal cations (that include, but are not limited to sodium, boron, barium, iron, silicon and calcium) organo-metallic compounds, organic compounds such as polymers that leach from plastic components into water, anionic compounds, in particular those including chlorine and nitrate ions, and inorganic chemical compounds (e.g., ammonium nitrate), dissolved and particulate matter, and dissolved gases including dissolved oxygen.
  • biological for example, bacterial matter
  • metals and metal cations that include, but are not limited to sodium, boron, barium, iron, silicon and calcium
  • organo-metallic compounds organic compounds such as polymers that leach from
  • the chemical compounds may include volatile and non-volatile chemicals and could further include reactive and inert chemicals, hydrophilic and hydrophobic chemicals and the like.
  • the requirements of UPW quality are outlined in standards that include, but are not limited to, as the ASTM D5127 “Standard Guide for Ultra- Pure Water Used in the Electronics and Semiconductor Industries” and SEMI F63 “Guide for ultrapure water used in semiconductor processing.”
  • UPW may also be used in the pharmaceutical and biotechnology industries. UPW may be used for cleaning semiconductor parts and components and for sterilization.
  • Analysis of liquid sample including nanoparticle contaminants (analytes) using example system 100 may preferably include nebulizing the liquid sample to generate an aerosol including nanoparticles and water vapor and condensing water vapor onto the nanoparticles to increase or enlarge the size of the particles in condensation growth tubes for analysis using LDLMS or other surface -based analytic methods.
  • the aerosol including the large “grown” particles may then be deposited onto sample disk 112 at station 103 or concentrated by means of a virtual impactor as discussed above.
  • Sample disk 112 may be fabricated from or coated with a metal that is not considered a contaminant, thereby eliminating interference from the sample disk itself.
  • FIGS. 11 A and 1 IB show schematic diagrams of example nebulizers for use in an example autonomous aerosol sample capture and analysis system, according to some implementations.
  • the carrier gas flow may be perpendicular to the liquid sample flow as shown in FIG. 11 A.
  • the nebulizer may be in the form of an annular tube including an orifice at the exit as shown in FIG. 11B. In this case, the liquid sample flows through the annulus and is forced out as an aerosol through the orifice by the carrier gas.
  • TSI, Inc. markets a range of products for nebulizing liquids.
  • the target nanoparticles in the aerosol exiting the nebulizer are condensed or grown as described below to at least about 3 pm in size to enable detection TOF-MS and other optical detectors.
  • Any method for concentrating the nanoparticles may be used.
  • the nanoparticles may be subjected to condensation to grow particles to a size that can be detected optically.
  • condensational growth of ultrafine particles may be done using (1) adiabatic expansion, (2) turbulent mixing, or (3) cold-walled condenser tubes. Each of these methods creates a region of super-saturation, wherein the concentration of the condensing vapor is greater than its equilibrium vapor pressure at the local gas temperature.
  • U.S. Pat. No. 6,712,881 “CONTINUOUS, LAMINAR FLOW WATERBASED PARTICLE CONDENSATION DEVICE AND METHOD,” discloses a method wherein an aerosol, or aerosol plus particle-free sheath air, flows in a laminar manner through a device or tube whose walls are wetted and held at a temperature warmer than the entering flow. Because the mass diffusivity of water vapor is larger than the thermal diffusivity of air, the transport of water vapor from the warm, wetted walls is faster than the rate at which the flow warms.
  • the relatively faster diffusion of water vapor from the warm, wet walls as compared to the warming of the flow creates a region of supersaturation, particle activation and condensational growth.
  • a particle-free sheath air may be used to surround the particle-laden aerosol flow so as to confine the particles to the centerline, where the highest super-saturation is achieved.
  • Aerosol Devices (Fort Collins, Colorado) provides a condensation growth tube system that could be integrated into system 100.
  • the ‘421 U.S. Patent discloses a method including the steps of: providing a growth chamber having walls at a first temperature, providing a warm sheath flow containing a high concentration of a condensable vapor (water vapor in a carrier gas such as air) at a second temperature lower than the first temperature and introducing an laminar aerosol flow with the sheath flow, wherein the aerosol flow temperature is at a third temperature lower than the first temperature and the second temperature.
  • This sheath flow is introduced in a laminar manner to surround the colder aerosol flow.
  • a region of vapor supersaturation is created as a result of the differential rates of thermal and mass transport.
  • the vapor condenses on the particles, enlarging them into droplets.
  • the sheath flow may be temperature conditioned prior to entering the condensation growth region. This method may be used to grow particles as small as 3.2 nm in diameter.
  • the condensable vapor may include methanol in a carrier gas including one or more of air, carbon dioxide, or argon.
  • U.S. Pat. No. 9,821,263 titled “ADVANCED LAMINAR FLOW WATER CONDENSATION TECHNOLOGY FOR ULTRAFINE PARTICLES,” discloses a method for growing aerosol particle size including introducing an airstream in a laminar flow into a wet- walled condenser having an inlet and an outlet, the airstream having an inlet temperature at the inlet of the condenser, controlling a first portion of the condenser adjacent to the inlet to a first temperature greater than the inlet temperature by at least 5° C, and controlling a second portion of the condenser between the first portion and the outlet at a second temperature lower than the first temperature.
  • the first portion and the second portion of the condenser define a volume, wherein introducing the airstream into the condenser creates a volumetric air flow rate within the volume, the volume having a cylindrical geometry or a multiple plate geometry wherein each plate has a width and a separation between plates.
  • the length of the first portion divided by the volumetric flow rate is less than 0.5 s/cm 2 and for a multiple plates geometry a quantity of the length of the first portion divided by the volumetric flow rate multiplied a quantity of the width divided by the separation is less than 0.5 s/cm 2 .
  • the walls of the condensation growth tubes may be wetted using a wick.
  • a siphoned wick may use a siphon-like method to maintain a water-filled gap behind the wick on a side of the wick opposite the air flow.
  • This approach may be supplemented with active pumping to accommodate large systems.
  • more than one condensation growth tube may be used to grow target aerosol particles of different sizes or diameters by controlling the temperature profiles in the growth tubes.
  • the aerosol exiting the nebulizer may be routed to the condensation growth tube.
  • the design (length, diameter and operating temperature) of the condensation growth tube may be optimized based on the particle load (counts) in the aerosol exiting the nebulizer, and the flow rate into the condensation growth tube and the flow rate of the carrier gas, which is typically filtered air.
  • the Aerosol Devices condensation growth tube system provides growth tubes that are about 4.5 in. in length and can process flow rates between about 1 LPM and 1.5 LPM per tube.
  • the BioSpot VIVAS (Aerosol Devices) can handle a flow of 8 LPM through 8 tubes.
  • the aerosol exiting the condensation growth tube may be deposited on to sample disk 112 as dry or wet particles.
  • Nozzle 1101 may be designed to generate the optimal impaction velocity of the target droplet.
  • the optimal velocity for nozzle diameters ranging from 0.35 - 0.7 mm is in the range of 50 - 100 m/s.
  • liquid water may be removed by drying the disk 112 as previously disclosed.
  • An important aspect of the nebulization is to entrain the aerosol in a low volume of filter air.
  • station 106 may include in addition to TOF-MS mass spectral analysis, one or more optical detection tools and methods, because when the analyte deposited sample on disk 112 absorbs sufficient light energy from a laser pulse, it emits characteristic photons as they transition from a high-energy state to a lower energy state and generate transient optical signatures such as high-order fluorescence, laser-induced breakdown spectroscopy (LIBS), Raman spectra, and infrared spectra. Therefore, in addition to mass spectrometry, optical sensors/detectors may be used to identify the composition of the sample particles.
  • LIBS laser-induced breakdown spectroscopy
  • Raman spectra Raman spectra
  • infrared spectra infrared spectra
  • Measured data collected using both TOF-MS and optical sensors may be processed using data fusion techniques to provide information on the composition of the sample analytes.
  • data fusion techniques By collecting information from a variety of detectors that include one or more optical methods and mass spectrometry, it is possible to filter and analyze the data associated with the sample using data fusion protocols to rapidly (close to real-time) identify the composition and type of particles with a high accuracy, sensitivity, and specificity.
  • Data from each of the measurements including one or more of TOF-MS, LIBS, Raman spectroscopy, or infrared spectroscopy may be transferred to a sensor data fusion engine where artificial intelligence tools including machine learning and deep learning may be employed to fully characterize the particles.
  • Raman spectroscopy does not employ target-specific reagents and has a potential to be rapid (approximately five minutes or less).
  • Raman spectroscopy provides information about molecular vibrations that can be used for sample identification and quantitation.
  • the technique involves focusing a laser beam (e.g., a UV laser source with wavelength between about 330 and about 360 nm) on a sample and detecting inelastic scattered light.
  • the majority of the scattered light is of the same frequency as the excitation source and is known as Rayleigh or elastic scattering.
  • a very small amount of the scattered light is shifted in energy from the laser frequency, due to interactions between the incident electromagnetic waves and the vibrational energy levels of the molecules in the sample.
  • a laser pulse e.g., from a high energy Nd:YAG laser with a wavelength of about 1064 nm
  • a laser pulse is focused on the particle to ablate a small amount of the particle to generate a plasma.
  • the analyte particle breakdown (dissociate) into ionic and atomic species.
  • characteristic atomic emission lines of the elements may be observed using an optical detector such as a CCD detector.
  • fluorescence spectroscopy the sample molecules are excited by irradiation at a certain wavelength and emit radiation of a different wavelength. The emission spectrum provides information for both qualitative and quantitative analysis.
  • station 106 may include in addition to TOF-MS mass spectral analysis, one or more optical detection tools and methods, because when the analyte deposited sample on disk 112 absorbs sufficient light energy from a laser pulse, it emits characteristic photons as they transition from a high-energy state to a lower energy state and generate transient optical signatures such as high-order fluorescence, laser-induced breakdown spectroscopy (LIBS), Raman spectra, and infrared spectra. Therefore, in addition to mass spectrometry, optical sensors/detectors may be used to identify the composition of the sample particles.
  • LIBS laser-induced breakdown spectroscopy
  • Raman spectra Raman spectra
  • infrared spectra infrared spectra
  • Measured data collected using both TOF-MS and optical sensors may be processed using data fusion techniques to provide information on the composition of the sample analytes.
  • data fusion techniques By collecting information from a variety of detectors that include one or more optical methods and mass spectrometry, it is possible to filter and analyze the data associated with the sample using data fusion protocols to rapidly (close to real-time) identify the composition and type of particles with a high accuracy, sensitivity, and specificity.
  • Data from each of the measurements including one or more of TOF-MS, LIBS, Raman spectroscopy or infrared spectroscopy may be transferred to a sensor data fusion engine where artificial intelligence tools including machine learning and deep learning may be employed to fully characterize the particles.
  • loader station 101 may include a sample disk loader cartridge or magazine 200 as shown in FIGS. 2A-2E. It may be necessary to apply a small compressive force on the stack of disks using a spring or push rod 206, and weight 207. The compressive force ensures that sample disk 108 will drop to the stub when the flexible arms or wings 201 are flexed outward. The magnetic force created by the permanent magnet at the end of stub 116 assists in dropping the sample disk 112 to stub 116, and ensures that as it mates with stub 116, it is positioned flat on stub 116.
  • an electrically conductive path between stub 116 and sample disk 112 is necessary for some analysis methods, such as MALDI TOFMS, such that electric current may be supplied to or withdrawn from sample disk 112 during the analysis.
  • stub 116 and sample disk 112 are made of conductive materials.
  • Stub 116 should be conductive in the MS (ion source). In the analysis station including MALDI TOFMS, stub 116 pushes the disk inside the ion source.
  • stub 116 may be made of an electrically insulative material.
  • stub 116 may be made of a ceramic material.
  • FIG. 23 shows a perspective view 2300 of a sample disk holder including a stub made of electrically insulative material, according to some implementations.
  • TOFMS station 106 (with reference to FIG. 1A) may include a high voltage contact plate 2302 that is configured to contact sample disk 2301.
  • stub 116 holding sample disk 112 and actuated by stepper motor and Z-axis linear actuator When stub 116 holding sample disk 112 and actuated by stepper motor and Z-axis linear actuator is inserted into component 1701 of sample disk unloader cartridge 1705, it causes flexible elements 1702 to flex outward, allowing sample disk 1712 to travel past retention hooks or tabs (not shown, see reference numeral 203 in FIGS. 2C-2D). As the stub is withdrawn from component 1701, the elements 1702 retract, and the retention hooks/tabs grab and retain sample disk 1712. Flexible elements 1702 of spent sample cartridge 1705 may be viewed as structurally distinct of the wings 201 in fresh sample disk cartridge 200 (FIG. 2B).
  • Wings 1705 include hooks that are configured to grab a spent sample disk and remove it from a stub after the disk has been inserted into the holder.
  • wings 201 are configured to remove as single disk (per stub insertion) from a holder or cartridge including one or more disks in a stacked arrangement. If additional spent sample disks 1712 are already in spent sample disk cartridge 1705, they are pushed up and into the cartridge when sample stub, holding disk 116, is inserted into bottom end 1701. The surface of each disk 1712 is separated from another disk on account of the geometrically capped shape of each disk. Brass ring 1707 may provide the compressive force (weight) to the stack of disks in the spent disk cartridge 1705 via the push road 1706 and dowel 1715.
  • FIG. 18 shows a perspective view of an example high-capacity coiled cartridge 1805 for use in the sample capture and analysis system, according to some implementations.
  • Cartridge 1805 may include a fresh sample disk cartridge as shown in FIGS. 2A-2E or a spent sample disk cartridge as shown in FIGS 17A-17C.
  • cartridge 1805 may include a sample disk loader component or compartment 1821 disposed at one end of a coiled tube.
  • the length of the coil tube may be changed depending on the desired sample disk capacity of the cartridge. For example, a coiled tube of about 18 in. to about 20 in. in length may hold about 100 sample disks.
  • Bottom end 1820 of cartridge 1805 may be configured to include a component (not shown) similar to component 1701 cartridge 1705 (FIGS. 17A-17C), that is configured to mechanically engage with stub 116 of sample disk holder 108.
  • the surface of each sample disk in cartridge 1805 may be separated from another disk on account of the geometrically capped shape of each disk.
  • the coiled tube may be extruded from a polymeric material such as Teflon (“PTFE”).
  • the bottom end 1820 of spent sample disk cartridge 1805 may be identical to that of spent sample disk cartridge 1705.
  • the bottom end 1820 of spent fresh disk cartridge 1805 may be identical to that of fresh sample disk cartridge 200.
  • a control mechanism may include an actuator, for example, a worm drive with a flexible/bendable “worm” configured to engage with a spring disposed between the “worm” and the disk loader compartment 1821.
  • the worm drive may be being driven by a stepper motor or by stored mechanical energy (e.g., a coiled rubber band).
  • sample disks may be moved through cartridge 1805 under the influence of a magnetic field.
  • the spent sample disk cartridge may be configured as a coiled tube of similar length to receive and hold the spent disks.
  • the spent sample cartridge configured as a coiled tube may include a cartridge end similar to 1720 to remove a spent sample disk from the stub.
  • system 100 may include a fresh sample disk station or substrate loader station 101 configured to receive a fresh disk cartridge having one or more fresh sample disks, a spent sample disk unloader station 102, an aerosol sample collection station 103, and a sample disk holder 108 including a stub 116.
  • the sample disk holder 108 may be configured to removably engage with the fresh sample disk cartridge to receive a fresh sample disk, engage with the spent sample disk unloader station to return a spent sample disk, hold a fresh sample disk or a spent sample disk, and move in at least two directions (X-Y-Z) orthogonal to each other using one or more of a stepper motor or actuator using a predetermined analysis sequence, and one or more analysis stations 106.
  • the aerosol sample collection station 103 may be configured to produce a sample spot on a fresh sample disk when the sample disk holder 108 is positioned at the aerosol sample collection station.
  • System 100’ may further include a microcontroller configured to run the predetermined analysis sequence.
  • the aerosol sample collection station may include an impactor nozzle 165 having a nozzle tip 167 disposed at a predetermined spacing above the fresh sample disk. Air to be sampled including the analyte particles may be drawn through the impactor nozzle at a predetermined air flow rate to produce a sample spot on the fresh sample disk 112 (FIG. 1C).
  • the spent sample disk unloader station may include a spent sample disk storage container and means to disengage the spent sample disk from the sample disk holder and transfer it to the storage container.
  • a spent sample disk may be disengaged from the sample disk holder using a mechanical “pick and place” arm mechanism (not shown) disposed in frame 107 of system 100,’ and may be placed in a suitable container.
  • the spent sample disk may be manually removed from the sample disk holder and placed in a suitable container or other storage vessel.
  • Each spent sample disk may be individually sealed.
  • the spent sample disk unloader station may include a spent sample disk cartridge that is configured to removably engage with the spent disk cartridge to receive and store a spent sample disk.
  • system 100’ may include a particle count sensor.
  • the particle counts sensor may be configured to determine an anomaly in the composition of ambient air. That is, the example particle counts sensor may be configured to determine an anomaly in any background environment including ambient air inside an enclosed space or partially enclosed space, and the like.
  • the composition and type of the threat agent or analyte hazardous substance may not be known prior to capture of a sample and analysis using system 100’.
  • the particle counts sensor may configured to output one or more of particle size distribution, mean particle diameter, total particle count per unit volume, fluorescence of particles, depolarization properties of the aerosol particles, particle velocity distribution, or target analyte to clutter particle ratio.
  • the particle count sensor may be disposed upstream of system sample collection station 103. Particle properties such as autofluorescence and depolarization may be used to provide information related to anomaly and particle counts. Clutter is a generic term for particles that are not analyte particles.
  • An example particle counts sensor that incorporates simultaneous measurement of optical particle sizing, fluorescence, and depolarization on a particle- by-particle basis is manufactured by Air Techniques International, Inc. (Maryland). Measurement techniques such as fluorescence and depolarization assist in distinguishing threat aerosol particles (analyte) from normal ambient aerosol particles (clutter), as making this determination based solely on size and counts per unit of time is unreliable.
  • the particle counts sensor component may be added in parallel or upstream of the sample collection station 103. Information about the size of particles associated with the threat aerosol particles may be used to optimize the collection of these particles. For example, particles with mean particle diameter of about 1pm require a higher velocity to drive impaction than particles with mean particle diameter of about 3pm. The mass of the about 3pm diameter particles is about 27 times greater than that of the about 1pm particles, assuming particle densities are comparable.
  • the operation of the pump used in aerosol collection station 103 may be adjusted to cause a higher air flow velocity through the nozzle for the 1pm particles or slowed down to decrease air flow velocity if the particles to be collected were larger in size (e.g., about 3pm).
  • the optical detector may be utilized to optimize the collection efficiency of aerosol particles based on the mean particle diameter of the threat aerosol particles.
  • the number density of threat particles, as measured by the optical detector may be used to determine the duration of the sampling period. For example, if the threat aerosol is a dense aerosol with a high particle loading of threat particles, a shorter sampling period may be sufficient to obtain a good sample; when the threat aerosol concentration is dilute, a longer sampling period would be required.
  • stub 116 may be made of an electrically conductive material.
  • the sample disk may be made of one or more of nickel or nickel alloys.
  • the sample disks may be pre-coated with a MALDI matrix chemical.
  • System 100’ may further include one or more of a camera station 104, a liquid chemical dispensing station 105, or a drying station.
  • the sample disks may include a unique identifier including one or more of a bar code, QR code (2-dimensional bar code), a numeric string, an alphanumeric string or micro identifier dots.
  • the micro-identifier dots may be created using metallic inks and may be marked along the perimeter of the disks for easy reading using, for example, a camera or suitable reader.
  • the dispensing station 105 may be configured to dispense between about 0.5 pl and about 2 pl of a liquid.
  • the liquid dispensed may include one or more of a MALDI matrix chemical, tri-fluoroacetic acid (“TFA”), formic acid, acetic acid, acetonitrile, methanol, ethanol, or water.
  • TFA tri-fluoroacetic acid
  • the liquid dispensed at station 105 may include about 70 vol.-% acetonitrile, about 15 vol.-% TFA, with the remaining being water.
  • the liquid may include about 70 vol.-% methanol, about 15 vol.-% TFA, with the remaining being water.
  • the volume of liquid dispensed may be between about 0.5 pl and about 1 pl to obtain a sample spot of about 1 mm diameter (with a nozzle 165 diameter of about 0.7 mm) on the sample disk.
  • the dispensing volume may need to be greater than the volume disclosed above, or the dispensing step may not to be repeated, to compensate for the rapid evaporation of the solvent.
  • the dispensing volume and nozzle diameter may be varied to achieve a spot of between about 0.5 mm and about 1.5 mm in spot size.
  • An example spot size may be characterized by a diameter of between about 0.5 mm and 0.8 mm.
  • system 100’ or a portion thereof may be disposed in a space with temperature and humidity control. Alternatively, temperature and humidity inside system 100’ may be monitored, and the dispense volume may be controlled accordingly.
  • the volume of liquid dispensed for an analysis may be scaled in proportion to the size of the analyte spot. As such, a smaller spot will require less liquid to be dispensed.
  • An example enclosure for system 100’ may include a sealed, temperature, and humidity-controlled case.
  • One or more desiccants or adsorbents loaded into a suitable adsorber component may be provided within the case to remove water and solvent vapors to ensure that system 100’ is warm and dry, and sample drying preferably occurs in a warm and dry ambient condition.
  • a dehumidification device may be incorporated within the case.
  • a heating and cooling system may be disposed within the case to maintain a consistent temperature within the case. In this context, “warm” may indicate between about 30 °C and about 45 °C and “dry” means below about 25% relative humidity.
  • the one or more analysis stations 106 may include one or more of a TOFMS, LDI-MS, MALDI-TOFMS, LIBS, Raman spectroscopy, fluorescence microscopy, surface enhanced RAMAN spectroscopy, scanning electron microscopy IR spectroscopy, or an optical detector.
  • the mass analyzer may be configured for pulsed ion extraction and may use a reflectron time-of-flight tube.
  • the analysis station includes LDLMS or MALDI-MS, the system may be configured such that multiple spectrums may be generated over a range of laser power levels.
  • the camera station 104 may be configured to receive at least one of a microscope camera and a digital camera.
  • the drying station may be configured to substantially dry the sample using one or more of inductive heating, resistive heating, flow of air, or vacuum, or combinations thereof.
  • Camera station 104 may include one or more of a microscope camera, a digital camera, a fluorescence microscope camera, a hyperspectral imaging camera, or a thermal imaging camera.
  • hyperspectral, infrared, and thermal imaging cameras may be able to more accurately measure the mass of sample collected and the degree to which the sample has been dried.
  • Stub 116 may form a vacuum tight seal with the vacuum chamber of a TOF-MS using an O-ring seal 119.
  • system 100’ may be configured to communicate with a remote server using at least one of wired communication and wireless communication wherein the output of the analysis station is transferred to the remote server and then to a data processing station.
  • the system may be configured to communicate with a data processing station using at least one of wired communication and wireless communication wherein the output of the analysis station is transferred to the data processing station for signal processing.
  • a signal processing algorithm may be used to determine if specific analytes are present in the sample at detectable concentration levels.
  • Figure 19A shows a schematic diagram of a method for collecting and analyzing aerosol analyte particles in air, according to some implementations.
  • Example method 1900 for collecting and analyzing aerosol analyte sample particles in air using example system 100 or 100’ may include loading a fresh sample disk onto the sample disk holder at the fresh sample disk loader station 101 in step 1901 and moving the sample disk holder having a fresh disk to the aerosol sample collection station 103 in step 1902.
  • aerosol particles are impacted onto the sample disk and may produce a sample spot size of about 1 mm in diameter on the sample disk in step 1903.
  • Example method may include step 1904 of moving the sample disk holder to the liquid chemical dispensing station 105 for treating the deposited aerosol particles with chemicals.
  • the sample may be substantially dried in step 1905.
  • Step 1906 may include moving the sample disk holder to the one or more analysis stations 106 and analyzing the sample in step 1907.
  • the sample disk holder may be moved to the spent sample disk unloader station in step 1908 to unload the spent sample disk from the sample disk holder.
  • the sample disk includes a unique identifier including one or more of a bar code, QR code (2- dimensional bar code), a numeric string, an alphanumeric string, or micro identifier dots disposed along the perimeter of the sample disk.
  • the unique identifier of each sample disk may be read, for example in step 1901 using one or more of a camera or a suitable reader to associate the unique identifier of a disk with the sample corresponding to that disk or with the step of analyzing the sample corresponding to that disk.
  • the step 1902 including moving the sample disk holder having a fresh disk to the aerosol collection station 103 step may be triggered by an anomaly in ambient air composition determined by one or more of the output of the particle counts sensor, a predetermined sampling schedule, or an event.
  • An event may include, for example, a manual command, for example, manually running the system in a mail room of an enterprise or in a postal office after fresh mail is loaded into a mail sorting machine.
  • the particle counts sensor may be configured to output one or more of particle size distribution, mean particle diameter, total particle count per unit volume, fluorescence of particles, depolarization properties of the aerosol particles, particle velocity distribution, or target analyte to clutter particle ratio.
  • the particle count sensor may be disposed upstream of system sample collection station 103. Particle properties such as autofluorescence and depolarization may be used to provide information related to anomaly and particle counts.
  • example method 1900 may further include a “smart sampling” protocol.
  • the “smart sampling” protocol may include tuning one or more of a sample collection period or a flow rate of air through the impactor based on the output of the particle counts sensor.
  • the flow rate of air through the impactor (see FIG. 1C and related description) may be inversely proportional to the mean particle diameter of the analyte particles in air.
  • the sample collection time period may be inversely proportional to the total particle count of the analyte particles per unit volume of air. In some implementations, the sample collection period may be about 30 s.
  • the flow rate of air through the impactor may be about 3 L/min.
  • FIG. 19B shows a schematic diagram of an example method for dual-stage sample analysis using TOFMS and mass spectra processing, according to some implementations.
  • the analyzing the sample step 1907 in method 1900 may further including the steps of analyzing the sample using TOFMS in step 1909 and generating TOFMS raw spectral data unique to the aerosol analyte particles in step 1910.
  • Step 1910 may include generating a first set of raw spectral data by subjecting the sample to a first batch of laser ionization pulses in step 1911.
  • the first batch of laser pulses may be characterized by a first laser pulse energy (microjoules per pulse).
  • the first laser pulse energy may be about 5 microjoules per pulse.
  • Step 1910 may include the step of and generating a second set of raw spectral data in step 1912 by subjecting the sample to a second batch of laser ionization pulses having a pulse energy that is greater than the first laser pulse energy.
  • the second batch of laser ionization pulses may be characterized by a pulse energy of between about 15 microjoules per pulse and 25 microjoules per pulse.
  • a nitrogen gas laser (for example, supplied by LTB Lasertechnik Berlin Gmbh, Germany) may be used for ionizing samples including the MALDI matrix chemicals as described herein.
  • a nitrogen laser may produce up to about 100 microjoules per pulse at a wavelength of about 337 nm, with pulse lengths below 10 ns.
  • Tuning the energy of the laser ionization pulses as described above may be used to discriminate between chemical analytes particles (smaller molecules) and biological analyte particle (larger particles) and to obtain raw spectral data with increases signal-to-noise (S/N) ratio.
  • the first hatch of laser beam pulser may be used to obtain a chemical spectrum in the molecular weight range of between about 100 Da to 1000 Da, which may then be matched to spectra of chemical threat agents in a chemical threat library.
  • the second batch of laser beam pulses may be used to obtain a second spectrum in the molecular weight range of between about 1000 Da and about 100,000 Da.
  • Biological molecules of interest may be peptides and proteins that have masses in the range from 1,000 to 100,000 Da. Certain polymers and waxes may also have masses in this range.
  • a single sample may be analyzed for both chemical threats such as fentanyl, and other low-volatility chemicals, and for biological hazards such as anthrax.
  • FIG. 24B shows a perspective view 2400B of an example sample disk, according to some implementations.
  • an ionization laser beam characterized by a diameter of between about 100 pm and about 300 pm may be used to strike the sample spot deposited on sample disk 2404 at the TOFMS analysis station.
  • the laser beam aim point 2411 may be characterized by an elliptical shape when the laser beam hits the sample spot at an angle.
  • the laser beam may be characterized by a size of about 500 pm x about 300 pm resulting in a beam area of about 0.12 mm 2 .
  • Sample disk rotation as discussed above, may increase the sample area exposed to the laser beam to about 0.21mm 2 , which represents an increase in sample area exposed to the laser beam by about 1.75x.
  • FIG. 24C shows a perspective view 2400C of another example sample disk, according to some implementations.
  • the sample disk holder 2405 may be offset relative to an aim point 2411 of an ionization laser beam associated with the TOFMS, and additionally, the stub may be rotated about the aim point using a rotation motor to expose a relatively larger sample area 2410 of the sample spot to the ionization laser beam during TOFMS analysis of the analyte sample.
  • the offset may be about 0.5 mm. With an offset of about 0.5 mm, the sample area 2410 exposed to the laser beam may be about 0.9 mm, which represents an increase sample area exposed to the laser beam by about 7.5x (compared to 0.12 mm 2 ).
  • example method 1900 may include the step of transferring the output of the TOFMS analysis station to a signal processing station for signal processing using at least one of wired communication and wireless communication.
  • the method may further include the use of a signal processing algorithm or method (for example, including steps 1913 and 1914 as described above) to determine if specific analytes are present in the sample at detectable concentration levels.
  • the raw spectral data may be processed by the signal processing algorithm.
  • example method 1900 may include the step 1915 of generating one or more data files corresponding to raw spectral data or processed spectral data and associating the one or more data files with one or more of a date stamp of the analyzing step and the unique identifier of the sample disk.
  • example method 1900 may include the step 1916 of transferring the one or more data files corresponding to the raw spectral data or processed spectral data to one or more of a local data server, a local data analysis server, a cloud-based data server, or a cloud-based data analysis server.
  • TOFMS system to a pre-determined value, initiating the analysis process and generating mass spectral data
  • the dispensing step may include positioning the disk under a second dispensing tube at station 105 for depositing the MALDI matrix solution onto the disk and substantially drying the matrix chemical.
  • the initiating the analysis process step (f) may include the steps of evacuating the MS station 106 with the sample disk holder 108 sealed to it to less than about 10‘ 5 torr, and preferably less than about 5x10‘ 6 torr, applying a voltage to the electrically conductive disk to generate a strong electric field in the region near the surface of the disk, focusing a laser pulse to vaporize the MALDI matrix and analytes and generating ions, and accelerating the ions to the detector by controlling the direction of the electrical field.
  • Biological molecules of interest sometimes called “biomarkers,” are usually peptides and proteins that have masses in the range from 1,000 to 100,000 Da.
  • the example methods and systems disclosed above may also be used for analysis of liquid samples and a variety of samples, that include, but are not limited to suspicious or unknown powders or pills dissolved or suspended in a liquid, for example, methanol.
  • An aliquot of this liquid may be dispensed onto a sample disk and analyzed for chemical and biological hazards.
  • a pipettor may be used to dispense the aliquot onto the sample disk manually.
  • a dispensing pump may be used within an automated sample injection module.
  • a signal processing algorithm may be used to determine if specific analytes are present in an unknown sample at detectable concentration levels.
  • Step 21 A shows a schematic diagram 2100A for identifying an unknown analyte in a sample, according to some implementations.
  • Step 2101 includes generating raw spectral data and average (see steps 1912 and 1912’)-
  • step 2102 process averaged spectra is generated using one or more baseline subtraction, signal to noise ratio estimation and filtering/smoothing.
  • step 2103 the processed average spectra is compared with reference spectra in a library or spectra from a machine learning engine.
  • step 21104 Key spectral features may be compared for predicting composition of the biological or chemical agent in step 2104.
  • step 2105 one or more data files associated with a unique identifier of the sample and analytical reports may be transferred and saved to a server, which may be a local computer or cloud-based server.
  • Reference spectra or spectral features of one or more chemical or biological agents for use in step 2103 may be generated using a machine learning engine.
  • Figure 21B shows a schematic diagram 2100B for generating reference spectra and a runtime machine learning model, according to some implementations.
  • training spectral data of reference chemical and biological threat agents which may include critical characteristic features of the agents, for a machine learning (“ML”) engine.
  • FIG. 20B shows a characteristic mass spectral features for fentanyl captured from air.
  • Step 2107 includes identifying the number of laser pulses per batch and pulse energy (microjoules/pulse) for generating the reference spectra of agents.
  • Step 2108 includes generating raw spectral data under different laser operating conditions (number of pulses and energy per pulse) and generating process averaged reference spectra using one or more baseline subtraction, signal to noise ratio estimation and filter/smoothing.
  • Step 2019 includes generating reference spectrum for each agent and identifying key spectral features.
  • Step 2110 may include developing a training model using data measured from unknown samples and samples captured from air using KNN or suitable predictive algorithms.
  • Step 2111 may include generating a runtime model that may be used for predicting an unknown agent in a sample.
  • an example a sample capture and analysis system for analyzing analyte particles in a liquid sample may include a fresh sample disk station or substrate loader station configured to receive a fresh disk cartridge having one or more fresh sample disks, a spent sample disk unloader station, a liquid sample acceptance station configured to receive the liquid sample, a liquid chemical dispensing station, and a sample disk holder including a stub.
  • the sample disk holder may be configured to removably engage with the fresh sample disk cartridge to receive a fresh sample disk, engage with the spent sample disk unloader station to return a spent sample disk, hold a fresh sample disk or a spent sample disk, and move in at least two directions (X-Y-Z) orthogonal to each other using one or more of a stepper motor or actuator using a predetermined analysis sequence.
  • the example sample capture and analysis system may include one or more analysis stations.
  • the liquid sample acceptance station may be configured to produce a sample spot on a fresh sample disk, and wherein the operation of the system is controlled using a microcontroller configured to run the predetermined analysis sequence.
  • the liquid sample acceptance station may be configured to receive a liquid sample from an aerosol collection device.
  • the liquid sample acceptance station may be configured to receive a liquid sample including exhaled breath.
  • the liquid sample acceptance station may be configured to receive a liquid sample from an aerosol collection device or from container including a solid material suspended or dissolved in a liquid.
  • FIG. 25A shows a perspective view 2500A of an example aerosol sample capture and analysis system, according to some implementations.
  • FIG. 25B shows a schematic top view 2500B of an arrangement of one or more stations in an example aerosol sample capture and analysis system, according to some implementations.
  • one or more stations including fresh disk cartridge 2501, camera station 2504, aerosol sample collection station 2505, solvent dispensing station 2506, drying station 2503, analysis station 2502, spent sample disk unloader station 2507 (all stations are not shown in the system of FIG.
  • Sample disk holder 2510 may be coupled to an actuator arm 2509 configured to move in a circular motion (0) and in vertical direction (Z-axis) using a stepper motor and a rotary actuator to engage with the one or more stations through an opening on gantry plate 2508 (not shown for simplicity) associated with each respective station.
  • An example sample capture and analysis system depicted in FIG. 25A may be compact and may be defined by a smaller footprint compared to the sample capture and analysis system shown in FIG. 1 A. In some instances, a footprint of an example sample capture and analysis system may be between about 10 in. and about 13 in. in diameter.
  • an example sample capture and analysis system depicted in FIG. 25A may be configured to improve throughput associated with the capture and analysis of an aerosol sample.
  • FIG. 25C shows a schematic top view 2500C of an arrangement of one or more stations in an example aerosol sample capture and analysis system, according to some implementations.
  • a first set of stations may be supported by a first semi-circular gantry plate 2508A and a second set of stations may be supported by a second semi-circular gantry plate 2508B.
  • first gantry plate 2508 A may support stations including fresh sample disk station 2501, camera station 2504, aerosol sample collection station 2505, and liquid chemical dispensing station 2506.
  • FIG. 25C shows a schematic top view 2500C of an arrangement of one or more stations in an example aerosol sample capture and analysis system, according to some implementations.
  • first set of stations may be supported by a first semi-circular gantry plate 2508A and a second set of stations may be supported by a second semi-circular gantry plate 2508
  • second first gantry plate 2508B may support stations including analysis station 2502 and spent sample disk unloader station 2507.
  • First sample disk holder 2510A may be coupled to an actuator arm (not shown for simplicity) configured to move in a circular motion (0) and in vertical direction (Z-axis) using a stepper motor and a rotary actuator to engage with the one or more stations associated with first gantry plate 2508A through an opening on gantry plate 2508A (not shown for simplicity) associated with each respective station.
  • second sample disk holder 2510B may be coupled to another actuator arm (not shown for simplicity) configured to move in a circular motion (0) and in vertical direction (Z-axis) using another stepper motor and another rotary actuator to engage with the one or more stations associated with second gantry plate 2508B through an opening on gantry plate 2508B (not shown for simplicity) associated with each respective station.
  • another actuator arm not shown for simplicity
  • another stepper motor and another rotary actuator to engage with the one or more stations associated with second gantry plate 2508B through an opening on gantry plate 2508B (not shown for simplicity) associated with each respective station.
  • Drying station 2503 may be accessible by both sample disk holders 2510A and 2510B and may serve as a disk transfer station.
  • an example analysis method may include energizing the rotary and Z-axis actuators work to rotate an actuator arm to move the sample disk holder 2510A to pick up a fresh disk from fresh sample disk station 2501 , engaging with camera station 2504 to inspect the disk for quality control purposes, engaging with aerosol sample collection station 2505 where an aerosol sample is deposited as a sample spot on a the sample plate (as previously described herein), engaging with camera station 2504 to inspect the sample spot for quality control purposes, engaging with liquid chemical dispensing station 2506 for treating the sample spot with a solvent or MALDI matrix, engaging with camera station 2504 to inspect the sample spot for quality control purposes engaging with drying station 2503 to dry the sample in a controlled manner as previously described herein.
  • the sample plate at drying station 2503 may be picked up by sample disk holder 2510B and moved to analysis station 2502 for sample analysis.
  • Analysis station 2502 may include a TOFMS analysis station, as previously described herein. After analysis, the sample disk may be moved to spent sample disk unloader station 2507, which may complete the analysis operation. In the meantime, during sample analysis at station 2502, the first sample disk holder may continue with picking-up a new sample disk at station 2501 for collecting a new sample.
  • additional analysis stations may be associated with second gantry plate 2508B.
  • Example additional analysis stations may be associated with an LDI-MS, LIBS, Raman spectroscopy, fluorescence microscopy, surface enhanced RAMAN spectroscopy, scanning electron microscopy IR spectroscopy, or an optical detector.
  • the fresh sample disk station 2501, the liquid chemical dispensing station 2506, and the spent sample disk unloader station 2507 may be accessible to a user or technician through an access door of an example sample capture and analysis system.
  • EXAMPLE 1 Detection of Biological Aerosol Particles Using Example System 100
  • samples including Bacillus subtilis var niger (“Bg”), Bacillus thuringiensis (“Bt”), Escherichia coli, Enterobacteria phage T2 virus, and an albumin (protein) with molecular weight of 66 kDa were deposited on MALDI coated disks.
  • the samples were then treated with a solution including 70% methanol, 10% TFA and 15% water.
  • the samples were dried and analyzed using example system 100 that included a TOFMS. In each case, the sample was dried, the sample stub including the disk with the sample was moved to MS station 106 and evacuated in the MS system.
  • FIG. 5 shows raw (unprocessed) mass spectra for each of these samples under two conditions: (a) samples that were not substantially dried (or ineffectively dried) and samples that were effectively dried by evacuating at ⁇ about 10 s torr., according to some implementations.
  • effectively dried samples in each case resulted in increased signal intensity of peaks (relative abundance of fragmented ions), peak sharpening, identification of new fragments, and a spectral fingerprint that allowed for better comparison with spectral libraries compared to the samples that were not effectively dried.
  • a sample including biological aerosol particles including spores, vegetative bacteria, and virus was drawn into a chamber of volume of about 250 liters and nebulized using make-up air to achieve a particle concentration of 1000 parts per liter (ppi).
  • the chamber included a mixing fan to stir and mix the sampled air to get a homogeneous sample of aerosol particles in air.
  • a sample from the chamber was then drawn and a flow rate of about 4 liters per min (LPM) aerosol particles were deposited on a MALDI coated disk by impaction.
  • LPM flow rate of about 4 liters per min
  • an inline APS/Fluorescence sensor was used to measure particle size distribution, count the number of bioparticles (fluorescent), count the number of non-biological particles and to determine the target particle to clutter ratio. The sample was then analyzed using TOFMS. Sensitivity of between about 50 and about 100 spores in air was demonstrated.
  • EXAMPLE 3 High Specificity Detection of Biological Aerosol Particles Including Bg Spores Using Example System 100.
  • Aerosol samples including Bg spores were captured and analyzed using example system 100 and compared with library baseline reference Bg mass spectrum.
  • FIG. 6 shows processed mass spectrum (bottom) of Bg (Bacillus subtilis var niger) aerosol spores obtained using an example sample capture and analysis system and compared with library Bg spectra (top), according to some implementations. As shown in FIG. 6, the features of the measured spectrum highly correlated with that of the reference spectrum and demonstrated excellent specificity (mass resolution) capability of the example system and data processing tools.
  • Bg Bacillus subtilis var niger
  • EXAMPLE 4 High Specificity Detection of Biological Aerosol Particles Including Bt Spores Using Example System 100.
  • Aerosol samples including Bt (Bacillus thuringiensis al Hakam) spores were captured and analyzed using example system 100 and compared with library baseline reference Bt mass spectrum.
  • FIG. 7 shows processed mass spectrum (bottom) of Bt (Bacillus thuringiensis al Hakam) aerosol spores obtained using an example sample capture and analysis system and compared with library Bt spectra (top), according to some implementations.
  • Bt Bacillus thuringiensis al Hakam
  • EXAMPLE 5 High Specificity Detection of Biological Aerosol Particles Using Example System 100.
  • Aerosol samples including Bt, Bg, E. coli and albumin (66 kDa) were captured and analyzed using example system 100.
  • EXAMPLE 7 High Sensitivity Detection of Biological Aerosol Particles Including E. coli Using Example System 100.
  • Aerosol samples including E. coli were captured and analyzed using example system 100 and compared with baseline reference mass spectrum.
  • FIG. 14 shows processed mass spectrum of bioaerosol particles including E. coli using an example sample capture and analysis system, according to some implementations. As shown in FIG. 14, the features of the measured spectrum highly correlated with that of the reference spectrum and demonstrated excellent specificity (mass resolution) capability of the example system and data processing tools.
  • EXAMPLE 8 High Sensitivity Detection of Biological Aerosol Particles Including Y. rohdei Using Example System 100.
  • FIG. 15 shows processed mass spectrum of bioaerosol particles including Y. rohdei using an example sample capture and analysis system, according to some implementations. As shown in FIG. 15, the features of the measured spectrum highly correlated with that of the reference spectrum and demonstrated excellent specificity (mass resolution) capability of the example system and data processing tools.
  • FIG. 16 shows processed mass spectrum of bioaerosol particles including E. coli Bacteriophage MS2 virus using an example sample capture and analysis system, according to some implementations. As shown in FIG. 16, the features of the measured spectrum demonstrated excellent specificity (mass resolution) capability of the example system and data processing tools.
  • FIG. 20B shows processed TOFMS mass spectrum 2000 of aerosolized fentanyl captured from air using an example sample capture and analysis system, according to some implementations.
  • Fentanyl has a molecular weight of 337 Da.
  • the mass spectral fingerprint of the measured fentanyl spectrum demonstrated excellent specificity (mass resolution) and signal response.
  • FIG. 20A shows a TOFMS mass spectrum 2000 of MALDI matrix coated on a sample disk and analyzed using an example sample capture and analysis system, according to some implementations.

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Abstract

L'invention concerne des systèmes et des procédés pour assurer une détection rapide et autonome de particules d'analyte biologique et chimique dans des échantillons de gaz et de liquide. Des systèmes et des procédés pour capturer et identifier des particules d'analyte d'aérosol biologique et chimique à l'aide d'une spectrométrie de masse à désorption laser assistée par matrice (MALDI-MS) et à l'aide d'une spectrométrie de masse à temps de vol (TOFMS) sont divulgués. Une spécificité élevée pour la capture et la détection de fentanyl aérosolisé a été démontrée en utilisant un système portable de capture et d'analyse d'échantillon.
PCT/US2025/038725 2024-07-22 2025-07-22 Systèmes et procédés de détection rapide et autonome de particules d'aérosol Pending WO2026039155A2 (fr)

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