WO2026065363A1 - Réseau de détection d'informations spatiales d'acides nucléiques et procédé de détection - Google Patents

Réseau de détection d'informations spatiales d'acides nucléiques et procédé de détection

Info

Publication number
WO2026065363A1
WO2026065363A1 PCT/CN2024/122653 CN2024122653W WO2026065363A1 WO 2026065363 A1 WO2026065363 A1 WO 2026065363A1 CN 2024122653 W CN2024122653 W CN 2024122653W WO 2026065363 A1 WO2026065363 A1 WO 2026065363A1
Authority
WO
WIPO (PCT)
Prior art keywords
sequence
nucleic acid
molecule
probe
positioning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/CN2024/122653
Other languages
English (en)
Chinese (zh)
Inventor
廖莎
陈奥
徐讯
许军强
何颖
江霞
张曼玲
姜南
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stomics Tech Co Ltd
Original Assignee
Stomics Tech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stomics Tech Co Ltd filed Critical Stomics Tech Co Ltd
Priority to PCT/CN2024/122653 priority Critical patent/WO2026065363A1/fr
Publication of WO2026065363A1 publication Critical patent/WO2026065363A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B20/00—Methods specially adapted for identifying library members
    • C40B20/02—Identifying library members by their fixed physical location on a support or substrate
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Sustainable Development (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un réseau d'acides nucléiques pour détecter des informations spatiales d'acides nucléiques dans un échantillon, un procédé de détection d'informations spatiales d'acides nucléiques dans un échantillon sur la base du réseau, et un procédé de production du réseau d'acides nucléiques.
PCT/CN2024/122653 2024-09-30 2024-09-30 Réseau de détection d'informations spatiales d'acides nucléiques et procédé de détection Pending WO2026065363A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2024/122653 WO2026065363A1 (fr) 2024-09-30 2024-09-30 Réseau de détection d'informations spatiales d'acides nucléiques et procédé de détection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2024/122653 WO2026065363A1 (fr) 2024-09-30 2024-09-30 Réseau de détection d'informations spatiales d'acides nucléiques et procédé de détection

Publications (1)

Publication Number Publication Date
WO2026065363A1 true WO2026065363A1 (fr) 2026-04-02

Family

ID=99305876

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2024/122653 Pending WO2026065363A1 (fr) 2024-09-30 2024-09-30 Réseau de détection d'informations spatiales d'acides nucléiques et procédé de détection

Country Status (1)

Country Link
WO (1) WO2026065363A1 (fr)

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150344942A1 (en) * 2012-10-17 2015-12-03 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2018091676A1 (fr) * 2016-11-17 2018-05-24 Spatial Transcriptomics Ab Procédé de marquage spatial et d'analyse d'acides nucléiques dans un échantillon biologique
US20210155982A1 (en) * 2019-11-21 2021-05-27 10X Genomics, Inc. Pipeline for spatial analysis of analytes
CN113767177A (zh) * 2018-12-10 2021-12-07 10X基因组学有限公司 生成用于空间分析的捕获探针
CN114207105A (zh) * 2019-05-15 2022-03-18 深圳华大生命科学研究院 用于检测核酸空间信息的阵列及检测方法
CN114934110A (zh) * 2022-06-15 2022-08-23 江西烈冰生物科技有限公司 用于获取基因表达的原始位置的生物芯片、试剂盒及方法
CN115896252A (zh) * 2011-04-13 2023-04-04 空间转录公司 用于组织样本中核酸的局部或空间检测的方法和产品
WO2023154554A1 (fr) * 2022-02-14 2023-08-17 The University Of Chicago Matériaux et procédés de transcriptomique spatiale à grande échelle
US11761038B1 (en) * 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
WO2024138670A1 (fr) * 2022-12-30 2024-07-04 深圳华大生命科学研究院 Séquence splint et son utilisation dans la capture d'acides nucléiques
WO2024145579A1 (fr) * 2022-12-29 2024-07-04 Illumina, Inc. Matériaux et procédés de préparation de bibliothèque de transcriptomique spatiale

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115896252A (zh) * 2011-04-13 2023-04-04 空间转录公司 用于组织样本中核酸的局部或空间检测的方法和产品
US20150344942A1 (en) * 2012-10-17 2015-12-03 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2018091676A1 (fr) * 2016-11-17 2018-05-24 Spatial Transcriptomics Ab Procédé de marquage spatial et d'analyse d'acides nucléiques dans un échantillon biologique
CN113767177A (zh) * 2018-12-10 2021-12-07 10X基因组学有限公司 生成用于空间分析的捕获探针
CN114207105A (zh) * 2019-05-15 2022-03-18 深圳华大生命科学研究院 用于检测核酸空间信息的阵列及检测方法
US20210155982A1 (en) * 2019-11-21 2021-05-27 10X Genomics, Inc. Pipeline for spatial analysis of analytes
US11761038B1 (en) * 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
WO2023154554A1 (fr) * 2022-02-14 2023-08-17 The University Of Chicago Matériaux et procédés de transcriptomique spatiale à grande échelle
CN114934110A (zh) * 2022-06-15 2022-08-23 江西烈冰生物科技有限公司 用于获取基因表达的原始位置的生物芯片、试剂盒及方法
WO2024145579A1 (fr) * 2022-12-29 2024-07-04 Illumina, Inc. Matériaux et procédés de préparation de bibliothèque de transcriptomique spatiale
WO2024138670A1 (fr) * 2022-12-30 2024-07-04 深圳华大生命科学研究院 Séquence splint et son utilisation dans la capture d'acides nucléiques

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