WO2026073213A1 - Techniques de capture de protéines avec aptamères liés en surface - Google Patents

Techniques de capture de protéines avec aptamères liés en surface

Info

Publication number
WO2026073213A1
WO2026073213A1 PCT/US2025/048685 US2025048685W WO2026073213A1 WO 2026073213 A1 WO2026073213 A1 WO 2026073213A1 US 2025048685 W US2025048685 W US 2025048685W WO 2026073213 A1 WO2026073213 A1 WO 2026073213A1
Authority
WO
WIPO (PCT)
Prior art keywords
aptamers
aptamer
linkers
bound
cognate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2025/048685
Other languages
English (en)
Inventor
David Jones
Nithya Subramanian
Andrew Slatter
Michael DORWART
Michael Riel-Mehan
Gaetano SPECIALE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of WO2026073213A1 publication Critical patent/WO2026073213A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un complexe de capture à base d'aptamères. Le complexe de capture à base d'aptamères peut comprendre une surface solide et une pluralité d'aptamères couplés à une première partie de la surface solide par l'intermédiaire d'une première pluralité de lieurs. La pluralité d'aptamères se lie à des cibles apparentées. Dans certains modes de réalisation, le complexe de capture à base d'aptamères peut comprendre une seconde pluralité de lieurs couplés à une seconde partie de la surface solide. La première pluralité de lieurs comprend des groupes fonctionnels différents de ceux de la seconde pluralité de lieurs.
PCT/US2025/048685 2024-09-30 2025-09-30 Techniques de capture de protéines avec aptamères liés en surface Pending WO2026073213A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202463701176P 2024-09-30 2024-09-30
US63/701,176 2024-09-30

Publications (1)

Publication Number Publication Date
WO2026073213A1 true WO2026073213A1 (fr) 2026-04-02

Family

ID=97600223

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2025/048685 Pending WO2026073213A1 (fr) 2024-09-30 2025-09-30 Techniques de capture de protéines avec aptamères liés en surface

Country Status (1)

Country Link
WO (1) WO2026073213A1 (fr)

Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (fr) 1989-10-26 1991-05-16 Sri International Sequençage d'adn
WO2004018497A2 (fr) 2002-08-23 2004-03-04 Solexa Limited Nucleotides modifies
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2007123744A2 (fr) 2006-03-31 2007-11-01 Solexa, Inc. Systèmes et procédés pour analyse de séquençage par synthèse
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7329492B2 (en) 2000-07-07 2008-02-12 Visigen Biotechnologies, Inc. Methods for real-time single molecule sequence determination
US20080108082A1 (en) 2006-10-23 2008-05-08 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US20090042206A1 (en) * 2007-01-16 2009-02-12 Somalogic, Inc. Multiplexed Analyses of Test Samples
WO2012061832A1 (fr) 2010-11-05 2012-05-10 Illumina, Inc. Liaison entre des lectures de séquences à l'aide de codes marqueurs appariés
US20120208724A1 (en) 2011-02-10 2012-08-16 Steemers Frank J Linking sequence reads using paired code tags
US20120208705A1 (en) 2011-02-10 2012-08-16 Steemers Frank J Linking sequence reads using paired code tags
WO2014142841A1 (fr) 2013-03-13 2014-09-18 Illumina, Inc. Dispositifs fluidiques multicouches et leurs procédés de fabrication
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US20170328909A1 (en) * 2011-12-30 2017-11-16 Quest Diagnostics Investments Incorporated Aptamers and diagnostic methods for detecting the egf receptor
US20180187197A1 (en) * 2013-09-24 2018-07-05 Somalogic, Inc. Multiaptamer Target Detection
WO2020160627A1 (fr) * 2019-02-08 2020-08-13 Newsouth Innovations Pty Limited Composite et dispositif de détection pour bioanalyse de point d'intervention
WO2024191806A1 (fr) * 2023-03-10 2024-09-19 Illumina, Inc. Techniques de détection d'aptamères

Patent Citations (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (fr) 1989-10-26 1991-05-16 Sri International Sequençage d'adn
US7329492B2 (en) 2000-07-07 2008-02-12 Visigen Biotechnologies, Inc. Methods for real-time single molecule sequence determination
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2004018497A2 (fr) 2002-08-23 2004-03-04 Solexa Limited Nucleotides modifies
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US20100111768A1 (en) 2006-03-31 2010-05-06 Solexa, Inc. Systems and devices for sequence by synthesis analysis
WO2007123744A2 (fr) 2006-03-31 2007-11-01 Solexa, Inc. Systèmes et procédés pour analyse de séquençage par synthèse
US20080108082A1 (en) 2006-10-23 2008-05-08 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20090042206A1 (en) * 2007-01-16 2009-02-12 Somalogic, Inc. Multiplexed Analyses of Test Samples
WO2012061832A1 (fr) 2010-11-05 2012-05-10 Illumina, Inc. Liaison entre des lectures de séquences à l'aide de codes marqueurs appariés
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US20120208724A1 (en) 2011-02-10 2012-08-16 Steemers Frank J Linking sequence reads using paired code tags
US20120208705A1 (en) 2011-02-10 2012-08-16 Steemers Frank J Linking sequence reads using paired code tags
US20170328909A1 (en) * 2011-12-30 2017-11-16 Quest Diagnostics Investments Incorporated Aptamers and diagnostic methods for detecting the egf receptor
WO2014142841A1 (fr) 2013-03-13 2014-09-18 Illumina, Inc. Dispositifs fluidiques multicouches et leurs procédés de fabrication
US20180187197A1 (en) * 2013-09-24 2018-07-05 Somalogic, Inc. Multiaptamer Target Detection
WO2020160627A1 (fr) * 2019-02-08 2020-08-13 Newsouth Innovations Pty Limited Composite et dispositif de détection pour bioanalyse de point d'intervention
WO2024191806A1 (fr) * 2023-03-10 2024-09-19 Illumina, Inc. Techniques de détection d'aptamères

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BENTLEY ET AL., NATURE, vol. 456, 2008, pages 53 - 59

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