WO2026142995A1 - Procédés de production de bibliothèques de séquençage à cellule unique d'adn provenant de chromatine ouverte - Google Patents

Procédés de production de bibliothèques de séquençage à cellule unique d'adn provenant de chromatine ouverte

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Publication number
WO2026142995A1
WO2026142995A1 PCT/US2025/060882 US2025060882W WO2026142995A1 WO 2026142995 A1 WO2026142995 A1 WO 2026142995A1 US 2025060882 W US2025060882 W US 2025060882W WO 2026142995 A1 WO2026142995 A1 WO 2026142995A1
Authority
WO
WIPO (PCT)
Prior art keywords
dna
exogenous dna
nuclei
cells
attached
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2025/060882
Other languages
English (en)
Inventor
Robert MELTZER
Hannah RICKNER
Kristina FONTANEZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of WO2026142995A1 publication Critical patent/WO2026142995A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente divulgation concerne la production de bibliothèques de séquençage de polynucléotides moléculairement distincts. Sont fournis des procédés et des compositions liés à la production de bibliothèques, y compris des bibliothèques à cellule unique, comprenant de l'ADN provenant de régions de chromatine ouverte. Les procédés et les compositions peuvent être éventuellement liés à la production simultanée de bibliothèques de transcriptome, y compris des bibliothèques de transcriptome à cellule unique.
PCT/US2025/060882 2024-12-26 2025-12-22 Procédés de production de bibliothèques de séquençage à cellule unique d'adn provenant de chromatine ouverte Pending WO2026142995A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202463738876P 2024-12-26 2024-12-26
US63/738,876 2024-12-26

Publications (1)

Publication Number Publication Date
WO2026142995A1 true WO2026142995A1 (fr) 2026-07-02

Family

ID=98732885

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2025/060882 Pending WO2026142995A1 (fr) 2024-12-26 2025-12-22 Procédés de production de bibliothèques de séquençage à cellule unique d'adn provenant de chromatine ouverte

Country Status (1)

Country Link
WO (1) WO2026142995A1 (fr)

Citations (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
WO2005068656A1 (fr) 2004-01-12 2005-07-28 Solexa Limited Caracterisation d'acides nucleiques
US8053192B2 (en) 2007-02-02 2011-11-08 Illumina Cambridge Ltd. Methods for indexing samples and sequencing multiple polynucleotide templates
US20130274117A1 (en) 2010-10-08 2013-10-17 President And Fellows Of Harvard College High-Throughput Single Cell Barcoding
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9309502B2 (en) 2002-02-21 2016-04-12 Alere San Diego Inc. Recombinase polymerase amplification
WO2016130704A2 (fr) 2015-02-10 2016-08-18 Illumina, Inc. Procédés et compositions pour analyser des composants cellulaires
WO2018125982A1 (fr) 2016-12-29 2018-07-05 Illumina, Inc. Système d'analyse destiné à un accès orthogonal à des biomolécules dans des compartiments cellulaires et à leur étiquetage
US20210214721A1 (en) 2020-01-13 2021-07-15 Fluent Biosciences Inc. Reverse transcription during template emulsification
US11155810B2 (en) * 2017-05-26 2021-10-26 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US20210340596A1 (en) 2018-09-28 2021-11-04 Fluent Biosciences Inc. Target capture and barcoding in monodisperse droplets
US20220033810A1 (en) * 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US20220135966A1 (en) 2020-11-03 2022-05-05 Fluent Biosciences Inc. Systems and methods for making sequencing libraries
US11479816B2 (en) * 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
WO2022245868A1 (fr) 2021-05-18 2022-11-24 Fluent Biosciences Inc. Expression génomique perturbée dans des partitions instantanées préstructurées
WO2023196572A1 (fr) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Cytidine désaminases modifiées et méthodes d'utilisation

Patent Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683202B1 (fr) 1985-03-28 1990-11-27 Cetus Corp
US4683195B1 (fr) 1986-01-30 1990-11-27 Cetus Corp
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US9309502B2 (en) 2002-02-21 2016-04-12 Alere San Diego Inc. Recombinase polymerase amplification
WO2005068656A1 (fr) 2004-01-12 2005-07-28 Solexa Limited Caracterisation d'acides nucleiques
US8053192B2 (en) 2007-02-02 2011-11-08 Illumina Cambridge Ltd. Methods for indexing samples and sequencing multiple polynucleotide templates
US20130274117A1 (en) 2010-10-08 2013-10-17 President And Fellows Of Harvard College High-Throughput Single Cell Barcoding
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2016130704A2 (fr) 2015-02-10 2016-08-18 Illumina, Inc. Procédés et compositions pour analyser des composants cellulaires
WO2018125982A1 (fr) 2016-12-29 2018-07-05 Illumina, Inc. Système d'analyse destiné à un accès orthogonal à des biomolécules dans des compartiments cellulaires et à leur étiquetage
US11155810B2 (en) * 2017-05-26 2021-10-26 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US11479816B2 (en) * 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
US20210340596A1 (en) 2018-09-28 2021-11-04 Fluent Biosciences Inc. Target capture and barcoding in monodisperse droplets
US20210214721A1 (en) 2020-01-13 2021-07-15 Fluent Biosciences Inc. Reverse transcription during template emulsification
US20220033810A1 (en) * 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US20220135966A1 (en) 2020-11-03 2022-05-05 Fluent Biosciences Inc. Systems and methods for making sequencing libraries
WO2022245868A1 (fr) 2021-05-18 2022-11-24 Fluent Biosciences Inc. Expression génomique perturbée dans des partitions instantanées préstructurées
WO2023196572A1 (fr) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Cytidine désaminases modifiées et méthodes d'utilisation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FONTANEZ KRISTINA M. ET AL: "Intrinsic molecular identifiers enable robust molecular counting in single-cell sequencing", 5 October 2024 (2024-10-05), XP093387323, Retrieved from the Internet <URL:http://www.biorxiv.org/content/10.1101/2024.10.04.616561v1.full> [retrieved on 20260414], DOI: 10.1101/2024.10.04.616561 *

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