ZA896490B - One-step in situ hybridization assay - Google Patents

One-step in situ hybridization assay

Info

Publication number
ZA896490B
ZA896490B ZA896490A ZA896490A ZA896490B ZA 896490 B ZA896490 B ZA 896490B ZA 896490 A ZA896490 A ZA 896490A ZA 896490 A ZA896490 A ZA 896490A ZA 896490 B ZA896490 B ZA 896490B
Authority
ZA
South Africa
Prior art keywords
situ hybridization
hybridization assay
assay
copies
quantitative
Prior art date
Application number
ZA896490A
Other languages
English (en)
Inventor
Joel Bresser
Bresser Joel
Mary Jean Evinger-Hodges
Jean Evinger-Hodges Mary
Original Assignee
Res Dev Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Dev Foundation filed Critical Res Dev Foundation
Publication of ZA896490B publication Critical patent/ZA896490B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • AIDS & HIV (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Materials For Medical Uses (AREA)
  • Inorganic Insulating Materials (AREA)
  • Television Systems (AREA)
  • Stereo-Broadcasting Methods (AREA)
  • Valve Device For Special Equipments (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
ZA896490A 1988-08-31 1989-08-25 One-step in situ hybridization assay ZA896490B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23910688A 1988-08-31 1988-08-31

Publications (1)

Publication Number Publication Date
ZA896490B true ZA896490B (en) 1990-06-27

Family

ID=22900643

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA896490A ZA896490B (en) 1988-08-31 1989-08-25 One-step in situ hybridization assay

Country Status (12)

Country Link
EP (2) EP0432221B1 (da)
JP (1) JPH04502552A (da)
AT (1) ATE153705T1 (da)
AU (1) AU636854B2 (da)
DE (1) DE68928080T2 (da)
DK (1) DK34191A (da)
FI (1) FI910957A0 (da)
IL (1) IL91405A (da)
NZ (1) NZ230389A (da)
PT (1) PT91589B (da)
WO (1) WO1990002173A1 (da)
ZA (1) ZA896490B (da)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5985549A (en) * 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
WO1990002173A1 (en) * 1988-08-31 1990-03-08 Research Development Foundation One-step in situ hybridization assay
US6242184B1 (en) 1988-10-18 2001-06-05 University Of Massachusetts In-situ hybridization of single-copy and multiple-copy nucleic acid sequences
US6203977B1 (en) 1988-11-15 2001-03-20 Yale University Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization
AU646635B2 (en) * 1989-03-07 1994-03-03 Syngene, Inc. In-situ hybridization in suspension for detection or separation of cells
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
IL99025A0 (en) * 1990-08-15 1992-07-15 Astra Ab Novel process for the detection of pathogens using dna probes
US5629147A (en) * 1992-07-17 1997-05-13 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
BR9306867A (pt) * 1992-07-17 1998-12-08 Aprogenex Inc Enriquecimento e identificação de células fetais do sangue materno para a hibridização in situ
US5521061A (en) * 1992-07-17 1996-05-28 Aprogenex, Inc. Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies
EP0842298B1 (en) * 1995-07-28 2006-05-10 Rijksuniversiteit Groningen Methods and materials for determining relative abundance of microorganisms in mixed populations
JP2001509662A (ja) * 1996-04-19 2001-07-24 ファルマシア・アンド・アップジョン・カンパニー 疾患特異的遺伝子の定量および発見のための高容量ｉｎ―ｓｉｔｕ ｍＲＮＡ ハイブリダイゼーション法
EP1080228B1 (en) * 1998-05-18 2010-04-28 Igenex, Inc. In situ hybridization method for detecting target nucleic acid
JP4557347B2 (ja) * 2000-01-30 2010-10-06 株式会社カイノス 特定の塩基配列を有する標的核酸の検出またはその核酸量の測定方法
US20040115692A1 (en) * 2000-04-03 2004-06-17 Cytyc Corporation Methods, compositions and apparatuses for detecting a target in a preservative solution
ES2465948T3 (es) 2010-04-30 2014-06-09 Exiqon A/S Procedimiento de hibridación in situ y tampón
JP2023521356A (ja) * 2020-04-07 2023-05-24 アドヴァンスド セル ダイアグノスティクス，インコーポレイテッド ｉｎ ｓｉｔｕハイブリダイゼーションによって標的核酸を検出する方法及びそのキット
CN120948678B (zh) * 2025-10-17 2026-02-03 苏州药明康德新药开发有限公司 一种适用于质谱和荧光检测的rna药物稳定剂及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002173A1 (en) * 1988-08-31 1990-03-08 Research Development Foundation One-step in situ hybridization assay
AU646635B2 (en) * 1989-03-07 1994-03-03 Syngene, Inc. In-situ hybridization in suspension for detection or separation of cells

Also Published As

Publication number Publication date
IL91405A (en) 1996-03-31
IL91405A0 (en) 1990-04-29
PT91589A (pt) 1990-03-08
WO1990002173A1 (en) 1990-03-08
EP0432221A4 (en) 1992-01-15
FI910957A7 (fi) 1991-02-27
JPH04502552A (ja) 1992-05-14
ATE153705T1 (de) 1997-06-15
EP0357436A3 (en) 1991-06-12
AU636854B2 (en) 1993-05-13
AU4343989A (en) 1990-03-23
EP0357436A2 (en) 1990-03-07
DE68928080D1 (de) 1997-07-03
NZ230389A (en) 1991-11-26
DK34191D0 (da) 1991-02-27
EP0432221A1 (en) 1991-06-19
DK34191A (da) 1991-04-30
PT91589B (pt) 1995-08-09
DE68928080T2 (de) 1998-01-15
FI910957A0 (fi) 1991-02-27
EP0432221B1 (en) 1997-05-28

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