AR040593A1 - Metodos para usar polinucleotidos artificiales y composiciones de los mismos para reducir el silenciamiento de transgenes - Google Patents

Metodos para usar polinucleotidos artificiales y composiciones de los mismos para reducir el silenciamiento de transgenes

Info

Publication number
AR040593A1
AR040593A1 AR20030102566A ARP030102566A AR040593A1 AR 040593 A1 AR040593 A1 AR 040593A1 AR 20030102566 A AR20030102566 A AR 20030102566A AR P030102566 A ARP030102566 A AR P030102566A AR 040593 A1 AR040593 A1 AR 040593A1
Authority
AR
Argentina
Prior art keywords
polynucleotide
methods
plants
plant
divergent
Prior art date
Application number
AR20030102566A
Other languages
English (en)
Inventor
Stanislaw Flasinski
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=30770933&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR040593(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR040593A1 publication Critical patent/AR040593A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Los materiales y métodos descriptos proveen moléculas de polinucleótidos suficientemente divergentes de polinucleótidos de ocurrencia natural en plantas, o polinucleótidos introducidos previamente en plantas como transgenes, como para permitir la acumulación de caracteres en métodos de cruzamiento de plantas o métodos de transformación de plantas. La descripción también provee métodos y composiciones para detectar los polinucleótidos de la invención en plantas. Reivindicación 1: Un método para reducir el silenciamiento de transgenes en plantas transgénicas caracterizado porque comprende los pasos de: a) construir un polinucleótido artificial que es divergente de un polinucleótido conocido que codifica una proteína sustancialmente idéntica, y b) ensamblar una construcción de ADN que contiene dicha molécula de polinucleótido artificial; y c)transformar dicha construcción de ADN en una célula vegetal; y d) regenerar dicha célula vegetal en una planta transgénica fértil; donde dicho polinucleótido artificial y dicho polinucleótido conocido son divergentes si son menos de 85% idénticos y no tienen extensiones de secuencia de polinucleótidos de más de 23 nucleótidos con 100% de identidad.
AR20030102566A 2002-07-18 2003-07-16 Metodos para usar polinucleotidos artificiales y composiciones de los mismos para reducir el silenciamiento de transgenes AR040593A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US39666502P 2002-07-18 2002-07-18

Publications (1)

Publication Number Publication Date
AR040593A1 true AR040593A1 (es) 2005-04-13

Family

ID=30770933

Family Applications (1)

Application Number Title Priority Date Filing Date
AR20030102566A AR040593A1 (es) 2002-07-18 2003-07-16 Metodos para usar polinucleotidos artificiales y composiciones de los mismos para reducir el silenciamiento de transgenes

Country Status (11)

Country Link
US (4) US7615678B2 (es)
EP (1) EP1551966B1 (es)
CN (2) CN101508996B (es)
AR (1) AR040593A1 (es)
AU (3) AU2003247962B2 (es)
BR (1) BR0312771A (es)
CA (2) CA2492407C (es)
ES (1) ES2420929T3 (es)
MX (1) MXPA05000758A (es)
WO (1) WO2004009761A2 (es)
ZA (1) ZA200500434B (es)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003247962B2 (en) 2002-07-18 2008-06-12 Monsanto Technology Llc Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
DE60329236D1 (de) * 2003-07-07 2009-10-22 Univ Delhi South Campus Methode zur herstellung verbesserter fruchtbarkeitswiederherstellungslinien für sterile männliche transgene nutzpflanzen und eine dns-konstruktion zur benutzung in dieser methode
PT2308977E (pt) 2004-04-30 2013-05-13 Dow Agrosciences Llc Novo gene de resistência a herbicida
US20090209427A1 (en) * 2004-06-24 2009-08-20 Alibhai Murtaza F Microbial glyphosate resistant epsps
US7217867B2 (en) 2004-08-19 2007-05-15 Monsanto Technology Llc Eukaryotic translation initiation factor gene regulatory elements for use in plants
US20070199095A1 (en) 2005-10-13 2007-08-23 Edwards Allen Methods for producing hybrid seed
US8993846B2 (en) 2005-09-06 2015-03-31 Monsanto Technology Llc Vectors and methods for improved plant transformation efficiency
US8153598B2 (en) * 2005-10-19 2012-04-10 Intrexon Corporation PKD ligands and polynucleotides encoding PKD ligands
CN101553111B (zh) 2005-10-28 2013-06-26 美国陶氏益农公司 新除草剂抗性基因
EP2316957B1 (en) 2006-05-12 2014-09-10 Monsanto Technology LLC Methods and compositions for obtaining marker-free transgenic plants
EP2803728B1 (en) 2006-05-16 2018-11-21 Monsanto Technology LLC Use of non-agrobacterium bacterial species for plant transformation
US7855326B2 (en) 2006-06-06 2010-12-21 Monsanto Technology Llc Methods for weed control using plants having dicamba-degrading enzymatic activity
WO2007146678A1 (en) 2006-06-06 2007-12-21 Monsanto Technology Llc Method for selection of transformed cells
US7939721B2 (en) 2006-10-25 2011-05-10 Monsanto Technology Llc Cropping systems for managing weeds
US7838729B2 (en) * 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
EP2118289B1 (en) 2007-03-09 2013-12-11 Monsanto Technology, LLC Preparation and use of plant embryo explants for transformation
WO2009020824A1 (en) * 2007-08-03 2009-02-12 Pioneer Hi-Bred International, Inc. Polynucleotides encoding stop codons in multiple reading frames and methods of use
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US8158850B2 (en) 2007-12-19 2012-04-17 Monsanto Technology Llc Method to enhance yield and purity of hybrid crops
EP2300617B1 (en) 2008-07-16 2016-03-23 Monsanto Technology LLC Methods and vectors for producing transgenic plants
AR075549A1 (es) 2008-09-29 2011-04-20 Monsanto Technology Llc Evento transgenico de soja mon87705 y metodos para detectar el mismo
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
BR112012012404B1 (pt) 2009-11-23 2019-03-06 Monsanto Technology Llc "molécula de dna reconbinante de amplicon, sonda de dna, par de moléculas de dna mètodo para detectar a presença de uma molècula de dna e kit de detecçao de dna".
ES2641642T3 (es) 2010-03-08 2017-11-10 Monsanto Technology Llc Moléculas de polinucleótido para regulación génica en plantas
UY34014A (es) * 2011-04-15 2012-11-30 Dow Agrosciences Llc Genes sintéticos para expresar proteínas en células de maíz, construcciones, plantas transgénicas, métodos para controlar pestes y composiciones
EP2726493B1 (en) 2011-07-01 2020-10-14 Monsanto Technology LLC Methods and compositions for selective regulation of protein expression
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
CA2848695A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and composition for weed control comprising inhibiting ppg oxidase
EP3382027A3 (en) 2011-09-13 2018-10-31 Monsanto Technology LLC Methods and compositions for weed control
WO2013040057A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control
MX361938B (es) 2011-09-13 2018-12-19 Monsanto Technology Llc Métodos y composiciones para el control de malezas.
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
CN103183730B (zh) * 2011-12-27 2014-12-10 中国农业科学院作物科学研究所 一种与耐草甘膦相关蛋白及其编码基因与应用
AU2013205606B2 (en) * 2012-02-01 2015-05-21 Corteva Agriscience Llc Synthetic Brassica-derived chloroplast transit peptides
MX360866B (es) 2012-05-24 2018-11-09 A B Seeds Ltd Composiciones y métodos para silenciar la expresión genética.
CN102766609B (zh) * 2012-06-14 2013-10-23 重庆市农业科学院 一种抗草甘膦EPSP合成酶GmEPSPS及其编码基因与应用
CA2888264A1 (en) 2012-10-18 2014-04-24 Monsanto Technology Llc Methods and compositions for plant pest control
US9708589B2 (en) 2012-12-18 2017-07-18 Monsanto Technology Llc Compositions and methods for custom site-specific DNA recombinases
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
UY35252A (es) 2013-01-01 2014-07-31 Seeds Ltd Ab MÉTODOS PARA INTRODUCIR dsRNA EN SEMILLAS DE PLANTAS PARA MODULAR LA EXPRESIÓN GENÉTICA
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
BR112015023051A2 (pt) 2013-03-13 2017-11-14 Monsanto Technology Llc método para controle de ervas daninhas, composição herbicida, cassete de expressão microbiano e método de produção de polinucleotídeo
US10612019B2 (en) 2013-03-13 2020-04-07 Monsanto Technology Llc Methods and compositions for weed control
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
US10793867B2 (en) 2013-03-15 2020-10-06 Monsanto Technology, Llc Methods for targeted transgene-integration using custom site-specific DNA recombinases
US11039586B2 (en) 2013-03-15 2021-06-22 Monsanto Technology Llc Creation and transmission of megaloci
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
WO2014178932A1 (en) * 2013-05-02 2014-11-06 Syngenta Participations Ag Glyphosate resistant class i epsps genes
EP3007547B1 (en) * 2013-06-11 2018-07-18 Syngenta Participations AG Methods for generating transgenic plants
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
EP3030663B1 (en) 2013-07-19 2019-09-04 Monsanto Technology LLC Compositions and methods for controlling leptinotarsa
AU2014341879B2 (en) 2013-11-04 2020-07-23 Greenlight Biosciences, Inc. Compositions and methods for controlling arthropod parasite and pest infestations
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
WO2015108982A2 (en) 2014-01-15 2015-07-23 Monsanto Technology Llc Methods and compositions for weed control using epsps polynucleotides
WO2015153339A2 (en) 2014-04-01 2015-10-08 Monsanto Technology Llc Compositions and methods for controlling insect pests
CN106795515B (zh) 2014-06-23 2021-06-08 孟山都技术公司 用于经由rna干扰调控基因表达的组合物和方法
US11807857B2 (en) 2014-06-25 2023-11-07 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
CN106604993A (zh) 2014-07-29 2017-04-26 孟山都技术公司 用于控制昆虫害虫的组合物和方法
CN108064288B (zh) 2015-01-22 2021-11-26 孟山都技术公司 用于控制叶甲属的组合物和方法
BR112017015988B1 (pt) 2015-01-27 2024-02-27 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Métodos para a modificação sítio dirigida para um fragmento alvo de um gene alvo em uma planta inteira e para a obtenção de uma planta mutante livre de transgene
EP3095870A1 (en) 2015-05-19 2016-11-23 Kws Saat Se Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
EP3302053B1 (en) 2015-06-02 2021-03-17 Monsanto Technology LLC Compositions and methods for delivery of a polynucleotide into a plant
WO2016196782A1 (en) 2015-06-03 2016-12-08 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants
EA201890492A1 (ru) 2015-08-14 2018-08-31 Инститьют Оф Дженетикс Энд Девелопментал Байолоджи, Чайниз Акэдеми Оф Сайенсиз Способ получения риса, устойчивого к глифосату, путем сайт-направленной замены нуклеотида
CN108513584A (zh) 2015-08-28 2018-09-07 先锋国际良种公司 苍白杆菌介导的植物转化
WO2017059341A1 (en) 2015-10-02 2017-04-06 Monsanto Technology Llc Recombinant maize b chromosome sequence and uses thereof
US10003059B2 (en) * 2015-10-13 2018-06-19 Lawrence Livermore National Security, Llc Ion conductive inks and solutions for additive manufacturing of lithium microbatteries
WO2017083092A1 (en) * 2015-11-10 2017-05-18 Dow Agrosciences Llc Methods and systems for predicting the risk of transgene silencing
WO2017132330A1 (en) 2016-01-26 2017-08-03 Monsanto Technology Llc Compositions and methods for controlling insect pests
US10587005B2 (en) * 2016-03-30 2020-03-10 Wildcat Discovery Technologies, Inc. Solid electrolyte compositions
USD808490S1 (en) 2016-04-15 2018-01-23 Vista Outdoor Operations Llc Suppressor
CN109642235B (zh) 2016-06-28 2023-12-29 孟山都技术公司 用于植物中的基因组修饰的方法和组合物
WO2018005589A1 (en) * 2016-06-28 2018-01-04 Cellectis Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
CN106636025B (zh) * 2016-12-28 2017-11-14 四川天豫兴禾生物科技有限公司 一种水稻epsps突变体及其编码基因和应用
CN111263810A (zh) 2017-08-22 2020-06-09 纳匹基因公司 使用多核苷酸指导的核酸内切酶的细胞器基因组修饰
JP7578590B2 (ja) 2018-10-18 2024-11-06 インテリア セラピューティクス，インコーポレーテッド 第ｉｘ因子を発現するための組成物及び方法
SG11202107921SA (en) * 2019-02-12 2021-08-30 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
CN114280306B (zh) * 2021-05-27 2023-05-05 中国农业科学院植物保护研究所 牛筋草epsps蛋白elisa检测试剂盒及检测方法
CN115491384A (zh) * 2021-06-17 2022-12-20 杭州芳韵生物科技有限公司 一种表达植物源抗除草剂突变基因的载体及应用

Family Cites Families (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5094945A (en) 1983-01-05 1992-03-10 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use
DE3587548T2 (de) 1984-12-28 1993-12-23 Bayer Ag Rekombinante DNA, die in pflanzliche Zellen eingebracht werden kann.
US5176995A (en) 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
JP2615013B2 (ja) 1985-08-07 1997-05-28 モンサント コンパニ− グリホセート耐性キメラ遺伝子
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US4971908A (en) 1987-05-26 1990-11-20 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
US5312910A (en) 1987-05-26 1994-05-17 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
US5145783A (en) 1987-05-26 1992-09-08 Monsanto Company Glyphosate-tolerant 5-endolpyruvyl-3-phosphoshikimate synthase
US5229114A (en) 1987-08-20 1993-07-20 The United States Of America As Represented By The Secretary Of Agriculture Approaches useful for the control of root nodulation of leguminous plants
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
CA2024811A1 (en) 1989-02-24 1990-08-25 David A. Fischhoff Synthetic plant genes and method for preparation
US5310667A (en) 1989-07-17 1994-05-10 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5689041A (en) 1989-08-10 1997-11-18 Plant Gentic Systems N.V. Plants modified with barstar for fertility restoration
EP0426641B1 (en) 1989-10-31 2000-09-13 Monsanto Company Promoter for transgenic plants
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5543576A (en) 1990-03-23 1996-08-06 Mogen International Production of enzymes in seeds and their use
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
JP3173784B2 (ja) 1990-06-25 2001-06-04 モンサント カンパニー グリホセート耐性植物
US5633435A (en) * 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
ATE139566T1 (de) 1990-08-31 1996-07-15 Monsanto Co Glyphosattolerante 5-enolpyruvyl-3- phosphoshikimat-synthasen
AU9113791A (en) 1990-12-26 1992-08-17 Monsanto Company Control of fruit ripening and senescence in plants
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
FR2673643B1 (fr) 1991-03-05 1993-05-21 Rhone Poulenc Agrochimie Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide.
US5304730A (en) 1991-09-03 1994-04-19 Monsanto Company Virus resistant plants and method therefore
US5518908A (en) 1991-09-23 1996-05-21 Monsanto Company Method of controlling insects
CA2112999C (en) 1991-10-04 2007-04-24 Mark A. Conkling Pathogen-resistant transgenic plants
US6011199A (en) 1992-12-15 2000-01-04 Commonwealth Scientific Method for producing fruiting plants with improved fruit flavour
US6013864A (en) 1993-02-03 2000-01-11 Monsanto Company Plants resistant to infection by luteoviruses
JPH09506249A (ja) 1993-11-24 1997-06-24 モンサント・カンパニー 植物病原体の抑制方法
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6080560A (en) 1994-07-25 2000-06-27 Monsanto Company Method for producing antibodies in plant cells
US5750876A (en) 1994-07-28 1998-05-12 Monsanto Company Isoamylase gene, compositions containing it, and methods of using isoamylases
US5631152A (en) 1994-10-26 1997-05-20 Monsanto Company Rapid and efficient regeneration of transgenic plants
US5716837A (en) 1995-02-10 1998-02-10 Monsanto Company Expression of sucrose phosphorylase in plants
GB9515941D0 (en) * 1995-08-03 1995-10-04 Zeneca Ltd DNA constructs
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6946588B2 (en) 1996-03-13 2005-09-20 Monsanto Technology Llc Nucleic acid encoding a modified threonine deaminase and methods of use
US5958745A (en) 1996-03-13 1999-09-28 Monsanto Company Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants
US5773696A (en) 1996-03-29 1998-06-30 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
US6166292A (en) 1996-04-26 2000-12-26 Ajinomoto Co., Inc. Raffinose synthetase gene, method of producing raffinose and transgenic plant
GB9611981D0 (en) * 1996-06-07 1996-08-07 Zeneca Ltd Enhancement of gene expression
US5985605A (en) 1996-06-14 1999-11-16 Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada DNA sequences encoding phytases of ruminal microorganisms
US5998700A (en) 1996-07-02 1999-12-07 The Board Of Trustees Of Southern Illinois University Plants containing a bacterial Gdha gene and methods of use thereof
US6063756A (en) 1996-09-24 2000-05-16 Monsanto Company Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor
US6093695A (en) 1996-09-26 2000-07-25 Monsanto Company Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP
US6017534A (en) 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
US5942664A (en) 1996-11-27 1999-08-24 Ecogen, Inc. Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants
US6121436A (en) 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
EP1717322B1 (en) 1997-01-17 2012-07-18 Codexis Mayflower Holdings, LLC Evolution of whole cells and organisms by recursive sequence recombination
DE69837916T2 (de) 1997-04-03 2008-02-28 DeKalb Genetics Corp., DeKalb Verwendung von glyphosat-resistente maislinien
US6040497A (en) * 1997-04-03 2000-03-21 Dekalb Genetics Corporation Glyphosate resistant maize lines
US6171640B1 (en) 1997-04-04 2001-01-09 Monsanto Company High beta-conglycinin products and their use
US6380466B1 (en) 1997-05-08 2002-04-30 Calgene Llc Production of improved rapeseed exhibiting yellow-seed coat
HUP0001729A3 (en) * 1997-10-31 2002-04-29 Syngenta Participations Ag Glyphosate resistant transgenic plants
US6072103A (en) 1997-11-21 2000-06-06 Calgene Llc Pathogen and stress-responsive promoter for gene expression
US6063597A (en) 1997-12-18 2000-05-16 Monsanto Company Polypeptide compositions toxic to coleopteran insects
US6284948B1 (en) 1998-05-18 2001-09-04 Pioneer Hi-Bred International, Inc. Genes and methods for control of nematodes in plants
MXPA00012100A (es) 1998-06-05 2003-04-22 Calgene Llc Secuencias de acidos nucleicos relacionados con acil coenzima a: colesterol aciltransferasa.
WO1999065927A2 (en) 1998-06-17 1999-12-23 Maxygen, Inc. Method for producing polynucleotides with desired properties
CN1219064C (zh) 1998-08-04 2005-09-14 嘉吉有限公司 植物脂肪酸去饱和酶启动子
FR2784514B1 (fr) 1998-10-13 2001-04-27 Thomson Csf Procede de controle d'un laser semiconducteur unipolaire
US6531648B1 (en) 1998-12-17 2003-03-11 Syngenta Participations Ag Grain processing method and transgenic plants useful therein
JP2002534129A (ja) 1999-01-14 2002-10-15 モンサント テクノロジー エルエルシー ダイズ形質転換方法
WO2000070069A1 (en) 1999-05-13 2000-11-23 Monsanto Technology Llc Acquired resistance genes in plants
GB9921964D0 (en) * 1999-09-16 1999-11-17 Novartis Ag Organic compounds
MXPA02006021A (es) * 1999-12-16 2002-12-05 Monsanto Technology Llc Construcciones novedosas para expresion vegetal.
EP1325136A1 (en) * 2000-09-29 2003-07-09 Syngenta Limited Herbicide resistant plants
PL214848B1 (pl) 2000-09-29 2013-09-30 Monsanto Technology Llc Sposób wytwarzania tolerancyjnej na glifosat rosliny pszenicy, plodna tolerujaca glifosat roslina pszenicy, nasiono pszenicy, para czasteczek DNA, sposób wykrywania obecnosci diagnostycznej czasteczki DNA, sposób wykrywania sekwencji zawierajacej okreslone nukleotydy, sposób wytwarzania rosliny pszenicy z tolerancja glifosatu, zestaw do wykrywania DNA, nasiono transgenicznej pszenicy, roslina pszenicy wytworzona przez hodowle nasion, sposób wytwarzania rosliny pszenicy tolerujacej stosowanie glifosatu, sposób selektywnego kontrolowania chwastów na polu zawierajacym uprawe pszenicy, czasteczka DNA, roslina pszenicy tolerujaca glifosat, roslina zdolna do wytwarzania diagnostycznego amplikonu, nasiono, komórka
AU2003247962B2 (en) * 2002-07-18 2008-06-12 Monsanto Technology Llc Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
US8158850B2 (en) 2007-12-19 2012-04-17 Monsanto Technology Llc Method to enhance yield and purity of hybrid crops
BR112012012404B1 (pt) 2009-11-23 2019-03-06 Monsanto Technology Llc "molécula de dna reconbinante de amplicon, sonda de dna, par de moléculas de dna mètodo para detectar a presença de uma molècula de dna e kit de detecçao de dna".
EP2726493B1 (en) 2011-07-01 2020-10-14 Monsanto Technology LLC Methods and compositions for selective regulation of protein expression

Also Published As

Publication number Publication date
CN101508996B (zh) 2012-01-11
CA2851035C (en) 2018-05-29
EP1551966A2 (en) 2005-07-13
AU2008201016B2 (en) 2010-12-02
AU2010246509A1 (en) 2010-12-23
US20100138953A1 (en) 2010-06-03
US7919321B2 (en) 2011-04-05
CA2851035A1 (en) 2004-01-29
ZA200500434B (en) 2007-07-25
AU2010246509B2 (en) 2011-05-19
ES2420929T3 (es) 2013-08-27
CN1681923B (zh) 2010-06-02
AU2003247962B2 (en) 2008-06-12
EP1551966A4 (en) 2006-08-30
CN1681923A (zh) 2005-10-12
CN101508996A (zh) 2009-08-19
WO2004009761A2 (en) 2004-01-29
US9428764B2 (en) 2016-08-30
US20060191038A1 (en) 2006-08-24
WO2004009761A3 (en) 2004-07-29
US20110247103A1 (en) 2011-10-06
MXPA05000758A (es) 2005-04-28
AU2003247962A1 (en) 2004-02-09
US7615678B2 (en) 2009-11-10
BR0312771A (pt) 2005-05-03
EP1551966B1 (en) 2013-06-05
CA2492407C (en) 2014-09-30
US20140007288A1 (en) 2014-01-02
US8466270B2 (en) 2013-06-18
AU2008201016A1 (en) 2008-03-20
CA2492407A1 (en) 2004-01-29

Similar Documents

Publication Publication Date Title
AR040593A1 (es) Metodos para usar polinucleotidos artificiales y composiciones de los mismos para reducir el silenciamiento de transgenes
AR111197A2 (es) AXMI-031, AXMI-039, AXMI-040 Y AXMI-049, UNA FAMILIA DE GENES DE d-ENDOTOXINAS, Y MÉTODOS PARA USARLOS
KR970703695A (ko) 식물 바이러스 내성 유전자와 그 제조방법(Plant Virus Resistance Gene and Methods)
EA201170685A1 (ru) Гены, кодирущие токсины нематод
AR078052A1 (es) Gen plaguicida axmi 205 de chromobacterium y metodos para su uso
AR014155A1 (es) Proteina aislada involucrada en la resistencia a nematodos, secuencia de adn aislada, vector de transformacion, planta, semillas y metodo para aumentarla resistencia a nematodos en una planta
MX2009007308A (es) Plantas que tienen rasgos relacionados con el rendimiento mejorado y/o resistencia al estres abiotico aumentada y un metodo para obtener las mismas.
BRPI0606803A2 (pt) axmi-018, axmi-020 e axmi-021, uma famìlia de genes de delta-endotoxina e métodos para seu uso
AR074871A1 (es) Genes pesticidas de brevibacillus y metodos para su uso
ATE506443T1 (de) Verwendung einer nukleinsäure zur herstellung einer transgenen pflanze mit verbesserter toleranz gegenüber trockenheit
ES2483916T3 (es) RHD6 y su uso en la modulación del desarrollo del pelo radicular en plantas
AR096405A2 (es) Molécula de ácido nucleico que confiere tolerancia al herbicida dicamba, métodos para producir plantas tolerantes a dicamba y para métodos para controlar el crecimiento de malezas
AR051877A1 (es) Genes grg-8 que confieren resistencia a herbicidas
ATE319822T1 (de) Verfahren und materialien zur vorbeugung und behandlung von autoimmunkrankheiten
AR011740A1 (es) Proteinas del virus que produce enrollamiento en las hojas de la vid del tipo 2, secuencias de codificacion y metodos
AR039968A1 (es) Plantas resistentes a insectos y metodos para obtener las mismas
AR050085A1 (es) Procedimiento para la generacion de plantas transgenicas con resistencia aumentada a patogenos por modificacion del contenido y/o la actividad de factores despolimerizantes de actina.
BRPI0407296A (pt) ácidos nucléicos que codificam agentes antelmìnticos e plantas produzidas a partir deles
MX387173B (es) Genes de toxinas axmi669 y axmi991 y métodos para su uso.
AR077315A2 (es) Plantas con caracteristicas de crecimiento modificadas y metodo para obtener las mismas
AR011226A1 (es) Proteinas del virus que produce caries en los tallos del rupestris, secuencias de codificacion
AR120208A1 (es) Genes resistentes a insectos y métodos de uso
AR047991A1 (es) Plantas deficientes en la expresion de ttg3, acidos nucleicos, polipeptidos ttg3 y metodos para su utilizacion
AR040020A1 (es) Metodo para aumentar el tamano de organos y semillas en una planta
DK1499734T3 (da) Fremgangsmåde til fremstilling af miljömæssigt sikre transgene organismer

Legal Events

Date Code Title Description
FC Refusal