ATE105587T1 - Verfahren zur bestimmung von bakterienkonzentrationen mit einer abtrennung von nichtmikrobiellen zellen sowie ein apparat und reagenzmittel hierfuer. - Google Patents

Verfahren zur bestimmung von bakterienkonzentrationen mit einer abtrennung von nichtmikrobiellen zellen sowie ein apparat und reagenzmittel hierfuer.

Info

Publication number
ATE105587T1
ATE105587T1 AT8888304539T AT88304539T ATE105587T1 AT E105587 T1 ATE105587 T1 AT E105587T1 AT 8888304539 T AT8888304539 T AT 8888304539T AT 88304539 T AT88304539 T AT 88304539T AT E105587 T1 ATE105587 T1 AT E105587T1
Authority
AT
Austria
Prior art keywords
bacteria
atp
substrate
extraction medium
somatic cells
Prior art date
Application number
AT8888304539T
Other languages
English (en)
Inventor
Poul Erik Aegidius
Original Assignee
Foss Electric Holding As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Foss Electric Holding As filed Critical Foss Electric Holding As
Application granted granted Critical
Publication of ATE105587T1 publication Critical patent/ATE105587T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques
    • G01N2001/4061—Solvent extraction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT8888304539T 1987-05-21 1988-05-19 Verfahren zur bestimmung von bakterienkonzentrationen mit einer abtrennung von nichtmikrobiellen zellen sowie ein apparat und reagenzmittel hierfuer. ATE105587T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK258787A DK258787D0 (da) 1987-05-21 1987-05-21 Fremgangsmaade til bestemmelse af bakteriekoncentration i en proeve
EP88304539A EP0299601B1 (de) 1987-05-21 1988-05-19 Verfahren zur Bestimmung von Bakterienkonzentrationen mit einer Abtrennung von nichtmikrobiellen Zellen sowie ein Apparat und Reagenzmittel hierfür

Publications (1)

Publication Number Publication Date
ATE105587T1 true ATE105587T1 (de) 1994-05-15

Family

ID=8113417

Family Applications (1)

Application Number Title Priority Date Filing Date
AT8888304539T ATE105587T1 (de) 1987-05-21 1988-05-19 Verfahren zur bestimmung von bakterienkonzentrationen mit einer abtrennung von nichtmikrobiellen zellen sowie ein apparat und reagenzmittel hierfuer.

Country Status (5)

Country Link
US (1) US5258285A (de)
EP (1) EP0299601B1 (de)
AT (1) ATE105587T1 (de)
DE (1) DE3889505T2 (de)
DK (1) DK258787D0 (de)

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DE4136390C2 (de) * 1991-11-05 1995-03-09 D Prof Dr Kleiner Verfahren zur Schnellbestimmung von mikrobiologischen Aktivitäten in Nahrungsmitteln, kosmetischen und pharmazeutischen Zusammensetzungen und zellfreien Körperflüssigkeiten
DE9304954U1 (de) * 1992-04-13 1993-08-12 Biolac GmbH Gesellschaft für industrielle Nutzung von Milchinhaltsstoffen, 31097 Harbarnsen Analyse-Kit zur Bestimmung der bakteriellen Keimzahl in Molke und Molkeninhaltsstoffen
FI93742C (fi) * 1992-10-23 1995-05-26 Elias Hakalehto Menetelmä ja laite solujen osoittamiseksi
AT401526B (de) * 1993-02-10 1996-09-25 Scheirer Winfried Reagenzlösung zur stabilisierung der lumineszenz bei der luciferasemessung
JP3228812B2 (ja) * 1993-02-10 2001-11-12 日本マイクロリス株式会社 生菌数を測定する方法
JP3368985B2 (ja) * 1994-05-10 2003-01-20 バイエルコーポレーション 自動供給装置
US5736351A (en) * 1995-01-09 1998-04-07 New Horizons Diagnostics Corporation Method for detection of contaminants
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US5908751A (en) * 1996-04-26 1999-06-01 Toyo Ink Mfg. Co., Ltd. Method for detecting and/or determining ATP from microorganism cells in a sample
US5916802A (en) * 1997-02-19 1999-06-29 Fritz Berthold Device for measuring ATP
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Also Published As

Publication number Publication date
DE3889505T2 (de) 1994-08-25
EP0299601A1 (de) 1989-01-18
DE3889505D1 (de) 1994-06-16
EP0299601B1 (de) 1994-05-11
US5258285A (en) 1993-11-02
DK258787D0 (da) 1987-05-21

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