ATE113991T1 - Verfahren zur reinigung hochreiner heparinase in grosser menge. - Google Patents

Verfahren zur reinigung hochreiner heparinase in grosser menge.

Info

Publication number
ATE113991T1
ATE113991T1 AT89907019T AT89907019T ATE113991T1 AT E113991 T1 ATE113991 T1 AT E113991T1 AT 89907019 T AT89907019 T AT 89907019T AT 89907019 T AT89907019 T AT 89907019T AT E113991 T1 ATE113991 T1 AT E113991T1
Authority
AT
Austria
Prior art keywords
heparinase
approximately
purification
organisms
stabilized medium
Prior art date
Application number
AT89907019T
Other languages
English (en)
Inventor
Joseph J Zimmerman
Charles L Cooney
Original Assignee
Massachusetts Inst Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology
Application granted granted Critical
Publication of ATE113991T1 publication Critical patent/ATE113991T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT89907019T 1988-06-06 1989-06-02 Verfahren zur reinigung hochreiner heparinase in grosser menge. ATE113991T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US20323588A 1988-06-06 1988-06-06

Publications (1)

Publication Number Publication Date
ATE113991T1 true ATE113991T1 (de) 1994-11-15

Family

ID=22753090

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89907019T ATE113991T1 (de) 1988-06-06 1989-06-02 Verfahren zur reinigung hochreiner heparinase in grosser menge.

Country Status (6)

Country Link
EP (1) EP0420894B1 (de)
JP (2) JP2603349B2 (de)
AT (1) ATE113991T1 (de)
CA (1) CA1341079C (de)
DE (1) DE68919360T2 (de)
WO (1) WO1989012692A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714376A (en) * 1991-10-23 1998-02-03 Massachusetts Institute Of Technology Heparinase gene from flavobacterium heparinum
US5389539A (en) * 1992-11-30 1995-02-14 Massachusetts Institute Of Technology Purification of heparinase I, II, and III from Flavobacterium heparinum
DE4429660B4 (de) 1994-08-20 2004-02-12 Dade Behring Marburg Gmbh Zusatzmittel für diagnostische Tests zur Bestimmung der Gerinnungsfähigkeit des Blutes, Verfahren zur Reduzierung der Beeinflussung von diagnostischen Tests durch Heparin und Verwendung von Metallsalzen zu diesen Zwecken
EP1109919A2 (de) 1998-08-27 2001-06-27 Massachusetts Institute Of Technology Von heparinase i und ii abgeleitete heparinasen
SI1656455T1 (sl) * 2003-08-13 2012-12-31 Sandoz Ag Postopek za čiščenje rekombinantnih polipeptidov
JP5226697B2 (ja) * 2007-01-12 2013-07-03 ダウ グローバル テクノロジーズ エルエルシー 浸透圧によって細胞にショックを与えるための装置および方法
EP3561048A4 (de) 2016-12-22 2020-08-12 Shenzhen Hepalink Pharmaceutical Group Co., Ltd. Heparinase-produzierender pseudomonas-stutzeri-stamm und heparinase daraus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54107584A (en) * 1978-02-10 1979-08-23 Seikagaku Kogyo Co Ltd Preparation of pure heparinase
US4341869A (en) * 1980-08-25 1982-07-27 Massachusetts Institute Of Technology Process for producing heparinase

Also Published As

Publication number Publication date
JP2869039B2 (ja) 1999-03-10
WO1989012692A1 (en) 1989-12-28
DE68919360T2 (de) 1995-05-18
EP0420894A4 (en) 1992-03-18
EP0420894A1 (de) 1991-04-10
CA1341079C (en) 2000-08-08
JP2603349B2 (ja) 1997-04-23
JPH03505815A (ja) 1991-12-19
DE68919360D1 (de) 1994-12-15
JPH09149788A (ja) 1997-06-10
EP0420894B1 (de) 1994-11-09

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Legal Events

Date Code Title Description
EELA Cancelled due to lapse of time