ATE120238T1 - Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen. - Google Patents

Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen.

Info

Publication number
ATE120238T1
ATE120238T1 AT91902569T AT91902569T ATE120238T1 AT E120238 T1 ATE120238 T1 AT E120238T1 AT 91902569 T AT91902569 T AT 91902569T AT 91902569 T AT91902569 T AT 91902569T AT E120238 T1 ATE120238 T1 AT E120238T1
Authority
AT
Austria
Prior art keywords
protein
endotoxin protein
recovery
lysed cell
endotoxin
Prior art date
Application number
AT91902569T
Other languages
English (en)
Inventor
Leigh H English
Original Assignee
Ecogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecogen Inc filed Critical Ecogen Inc
Application granted granted Critical
Publication of ATE120238T1 publication Critical patent/ATE120238T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/803Physical recovery methods, e.g. chromatography, grinding
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/832Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT91902569T 1989-12-08 1990-12-04 Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen. ATE120238T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/447,966 US5173409A (en) 1989-12-08 1989-12-08 Recovery of bt endotoxin protein from lysed cell mixtures

Publications (1)

Publication Number Publication Date
ATE120238T1 true ATE120238T1 (de) 1995-04-15

Family

ID=23778470

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91902569T ATE120238T1 (de) 1989-12-08 1990-12-04 Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen.

Country Status (9)

Country Link
US (1) US5173409A (de)
EP (1) EP0504318B1 (de)
JP (1) JPH07500482A (de)
AT (1) ATE120238T1 (de)
AU (1) AU631724B2 (de)
CA (1) CA2070649A1 (de)
DE (1) DE69018093D1 (de)
DK (1) DK0504318T3 (de)
WO (1) WO1991009133A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW257792B (de) * 1992-10-01 1995-09-21 Lilly Co Eli
US6063756A (en) 1996-09-24 2000-05-16 Monsanto Company Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor
US6093695A (en) 1996-09-26 2000-07-25 Monsanto Company Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP
US6245551B1 (en) 1999-03-30 2001-06-12 Agraquest, Inc. Strain of Bacillus pumilus for controlling plant diseases caused by fungi
HUP0200562A2 (en) 1999-03-30 2002-06-29 Agraquest Inc A strain of baccilus pumilus for controlling plant diseases
RU2336307C2 (ru) 2000-01-19 2008-10-20 Мартек Биосайнсис Корпорейшн Способ получения липидов (варианты) и липиды, полученные этим способом
CN103124791B (zh) 2010-06-01 2016-08-24 帝斯曼知识产权资产管理有限公司 从细胞中提取脂质以及由此获得的产品
AU2014369042B2 (en) 2013-12-20 2020-04-30 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
JP6919827B2 (ja) 2013-12-20 2021-08-18 ディーエスエム ニュートリショナル プロダクツ アーゲーDSM Nutritional Products AG 微生物から油を回収する方法
KR102416853B1 (ko) 2013-12-20 2022-07-05 마라 리뉴어블즈 코퍼레이션 미생물로부터 오일을 회수하는 방법
NZ721409A (en) 2013-12-20 2022-10-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
WO2015095693A2 (en) 2013-12-20 2015-06-25 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
PL3082793T3 (pl) 2013-12-20 2020-10-05 Dsm Ip Assets B.V. Sposoby otrzymywania oleju drobnoustrojowego z komórek drobnoustrojowych
SG11201605043TA (en) 2013-12-20 2016-07-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells

Also Published As

Publication number Publication date
US5173409A (en) 1992-12-22
JPH07500482A (ja) 1995-01-19
AU631724B2 (en) 1992-12-03
DK0504318T3 (da) 1995-08-07
WO1991009133A1 (en) 1991-06-27
EP0504318A1 (de) 1992-09-23
CA2070649A1 (en) 1991-06-09
AU7166791A (en) 1991-07-18
DE69018093D1 (de) 1995-04-27
EP0504318B1 (de) 1995-03-22

Similar Documents

Publication Publication Date Title
ATE120238T1 (de) Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen.
EP0233044B1 (de) Verfahren zur Reinigung von rekombinanten Proteinen und die Verwendung von dessen Produkten
JPH06102034B2 (ja) タンパク質の生産方法
DE3688663D1 (de) Reinigung und aktivierung von proteinen aus unloeslichen einschlussteilchen.
JPS63105693A (ja) 細胞から親油性タン白の抽出方法
CA1267497C (en) PROTEIN RECOVERY
AU568265B2 (en) Process for preparation of a biologically active extract
SE8402861D0 (sv) Rening av biologiskt material
ATE86635T1 (de) Reinigung von rekombinantem interleukin-1.
DE3685487D1 (de) Enzyme fuer den abbau von lignin.
AU8210987A (en) N-alkyl and n-alkenyl aspartic acids as collectors for the flotation of non-sulfidic ores
KR870002258A (ko) 유전공학적 미생물로부터 정제된 성장호르몬 회수방법
Sakaguchi et al. Studies on toxin production of Clostridium botulinum type E III: Characterization of toxin precursor
KR860002571A (ko) 재조합 폴리펩티드의 분리 방법
KR900006512A (ko) 방 법
US4133904A (en) Treatment of single cell protein
BR9605847A (pt) Processo de preparação de um ácido orgánico
BG47043A3 (bg) Метод за изолиране на d-аминокиселинна оксидаза
US2927060A (en) Refining of proteolytic enzymes
KR910016926A (ko) 인간 β-인터페론의 정제방법
KR960031478A (ko) 유비퀴틴 융합 인간 칼시토닌의 정제방법
ATE220409T1 (de) Rückgewinnung von proteinen durch fällung mit ligninsulfonate
KR940014767A (ko) 아미노산 및 핵산 발효액으로부터 균체를 분리하는 방법
KR930013105A (ko) 양 성장 호르몬의 정제 방법
KR970006500A (ko) 재조합 효모로부터 발현된 감마 인터페론의 정제 방법

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties