ATE120238T1 - Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen. - Google Patents

Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen.

Info

Publication number
ATE120238T1
ATE120238T1 AT91902569T AT91902569T ATE120238T1 AT E120238 T1 ATE120238 T1 AT E120238T1 AT 91902569 T AT91902569 T AT 91902569T AT 91902569 T AT91902569 T AT 91902569T AT E120238 T1 ATE120238 T1 AT E120238T1
Authority
AT
Austria
Prior art keywords
protein
endotoxin protein
recovery
lysed cell
endotoxin
Prior art date
Application number
AT91902569T
Other languages
English (en)
Inventor
Leigh H English
Original Assignee
Ecogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecogen Inc filed Critical Ecogen Inc
Application granted granted Critical
Publication of ATE120238T1 publication Critical patent/ATE120238T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT91902569T 1989-12-08 1990-12-04 Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen. ATE120238T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/447,966 US5173409A (en) 1989-12-08 1989-12-08 Recovery of bt endotoxin protein from lysed cell mixtures

Publications (1)

Publication Number Publication Date
ATE120238T1 true ATE120238T1 (de) 1995-04-15

Family

ID=23778470

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91902569T ATE120238T1 (de) 1989-12-08 1990-12-04 Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen.

Country Status (9)

Country Link
US (1) US5173409A (de)
EP (1) EP0504318B1 (de)
JP (1) JPH07500482A (de)
AT (1) ATE120238T1 (de)
AU (1) AU631724B2 (de)
CA (1) CA2070649A1 (de)
DE (1) DE69018093D1 (de)
DK (1) DK0504318T3 (de)
WO (1) WO1991009133A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW257792B (de) * 1992-10-01 1995-09-21 Lilly Co Eli
US6063756A (en) * 1996-09-24 2000-05-16 Monsanto Company Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor
US6093695A (en) 1996-09-26 2000-07-25 Monsanto Company Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP
KR100736253B1 (ko) 1999-03-30 2007-07-06 아그라퀘스트 인코퍼레이티드 식물 질병을 방제하는 ｂａｃｉｌｌｕｓ ｐｕｍｉｌｕｓ의균주
US6245551B1 (en) 1999-03-30 2001-06-12 Agraquest, Inc. Strain of Bacillus pumilus for controlling plant diseases caused by fungi
KR101591834B1 (ko) 2000-01-19 2016-02-18 마텍 바이오싸이언스스 코포레이션 무용매 추출 방법
WO2011153246A2 (en) 2010-06-01 2011-12-08 Martek Biosciences Corporation Extraction of lipid from cells and products therefrom
CN105939710B (zh) 2013-12-20 2020-05-08 帝斯曼知识产权资产管理有限公司 用于从微生物细胞获得微生物油的方法
BR112016014262B1 (pt) 2013-12-20 2022-04-05 MARA Renewables Corporation Método para recuperar lipídeos de uma população de micro-organismos
NZ721413A (en) 2013-12-20 2022-05-27 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
TWI646189B (zh) 2013-12-20 2019-01-01 荷蘭商Ｄｓｍ智慧財產有限公司 用於從微生物細胞獲得微生物油之方法（五）
EP3083974A1 (de) 2013-12-20 2016-10-26 DSM Nutritional Products AG Verfahren zur rückgewinnung von öl aus mikroorganismen
AU2014369046B2 (en) 2013-12-20 2019-02-28 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
WO2015095688A1 (en) 2013-12-20 2015-06-25 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells

Also Published As

Publication number Publication date
WO1991009133A1 (en) 1991-06-27
JPH07500482A (ja) 1995-01-19
AU7166791A (en) 1991-07-18
EP0504318A1 (de) 1992-09-23
AU631724B2 (en) 1992-12-03
US5173409A (en) 1992-12-22
EP0504318B1 (de) 1995-03-22
CA2070649A1 (en) 1991-06-09
DE69018093D1 (de) 1995-04-27
DK0504318T3 (da) 1995-08-07

Similar Documents

Publication Publication Date Title
DE69018093D1 (de) Rückgewinnung von bt-endotoxinprotein aus lysierten zellmischungen.
EP0233044B1 (de) Verfahren zur Reinigung von rekombinanten Proteinen und die Verwendung von dessen Produkten
AU568265B2 (en) Process for preparation of a biologically active extract
JPH06102034B2 (ja) タンパク質の生産方法
DE3688663D1 (de) Reinigung und aktivierung von proteinen aus unloeslichen einschlussteilchen.
AU7201287A (en) Recombinant human endothelial cell growth factor
JPS63105693A (ja) 細胞から親油性タン白の抽出方法
ES2061876T3 (es) Activacion de anticuerpos obtenidos por ingenieria genetica expresados en procariotas.
EP0215625A3 (en) Protein recovery
SE8402861D0 (sv) Rening av biologiskt material
DE3685487D1 (de) Enzyme fuer den abbau von lignin.
AU8210987A (en) N-alkyl and n-alkenyl aspartic acids as collectors for the flotation of non-sulfidic ores
ATE111480T1 (de) Verfahren zur rückgewinnung rekombinanter proteine unter verwendung von flockungsmitteln.
KR870002258A (ko) 유전공학적 미생물로부터 정제된 성장호르몬 회수방법
Sakaguchi et al. Studies on toxin production of Clostridium botulinum type E III: Characterization of toxin precursor
KR860002571A (ko) 재조합 폴리펩티드의 분리 방법
KR900006512A (ko) 방 법
BR9605847A (pt) Processo de preparação de um ácido orgánico
BG47043A3 (bg) Метод за изолиране на d-аминокиселинна оксидаза
KR910016926A (ko) 인간 β-인터페론의 정제방법
JPS6342686A (ja) プロテア−ゼの製造法
KR960031478A (ko) 유비퀴틴 융합 인간 칼시토닌의 정제방법
ATE220409T1 (de) Rückgewinnung von proteinen durch fällung mit ligninsulfonate
KR940014767A (ko) 아미노산 및 핵산 발효액으로부터 균체를 분리하는 방법
KR930013105A (ko) 양 성장 호르몬의 정제 방법

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties