ATE121454T1 - Ubiquitinspezifische protease. - Google Patents

Ubiquitinspezifische protease.

Info

Publication number
ATE121454T1
ATE121454T1 AT91910453T AT91910453T ATE121454T1 AT E121454 T1 ATE121454 T1 AT E121454T1 AT 91910453 T AT91910453 T AT 91910453T AT 91910453 T AT91910453 T AT 91910453T AT E121454 T1 ATE121454 T1 AT E121454T1
Authority
AT
Austria
Prior art keywords
ubiquitin
peptide
protease
protein
moiety
Prior art date
Application number
AT91910453T
Other languages
English (en)
Inventor
Alexander J One Lon Varshavsky
John W Tobias
Original Assignee
Massachusetts Inst Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology
Application granted granted Critical
Publication of ATE121454T1 publication Critical patent/ATE121454T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT91910453T 1990-05-09 1991-05-08 Ubiquitinspezifische protease. ATE121454T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52108990A 1990-05-09 1990-05-09
US57395890A 1990-08-28 1990-08-28

Publications (1)

Publication Number Publication Date
ATE121454T1 true ATE121454T1 (de) 1995-05-15

Family

ID=27060368

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91910453T ATE121454T1 (de) 1990-05-09 1991-05-08 Ubiquitinspezifische protease.

Country Status (9)

Country Link
US (1) US5391490A (de)
EP (1) EP0531404B1 (de)
JP (1) JP3041644B2 (de)
AT (1) ATE121454T1 (de)
CA (1) CA2079229C (de)
DE (1) DE69109109T2 (de)
DK (1) DK0531404T3 (de)
ES (1) ES2071315T3 (de)
WO (1) WO1991017245A1 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212058A (en) * 1990-05-09 1993-05-18 Massachusetts Institute Of Technology Nucleic acid encoding ubiquitin-specific proteases
US5744343A (en) * 1994-01-04 1998-04-28 Mitotix, Inc. Ubiquitin conjugating enzymes
US5981699A (en) * 1994-01-04 1999-11-09 Mitotix, Inc. Human ubiquitin conjugating enzyme
EP0738394B1 (de) * 1994-01-04 2000-05-17 Mitotix, Inc. Ubiquitin-konjugierende enzyme
WO1997006247A2 (en) * 1995-08-09 1997-02-20 Dana Farber Cancer Institute Deubiquitinating enzymes that regulate cell growth
US5770720A (en) * 1995-08-30 1998-06-23 Barnes-Jewish Hospital Ubiquitin conjugating enzymes having transcriptional repressor activity
US6001619A (en) * 1995-10-04 1999-12-14 Cold Spring Harbor Laboratory Ubiquitin ligases, and uses related thereto
US5861483A (en) 1996-04-03 1999-01-19 Pro-Neuron, Inc. Inhibitor of stem cell proliferation and uses thereof
GB9618960D0 (en) 1996-09-11 1996-10-23 Medical Science Sys Inc Proteases
US6747128B2 (en) 1997-08-20 2004-06-08 Gpc Biotech, Inc. Components of ubiquitin ligase complexes, and uses related thereto
US5932422A (en) * 1997-11-14 1999-08-03 Millennium Pharmaceuticals, Inc. Modulation of drug resistance via ubiquitin carboxy-terminal hydrolase
US7668658B2 (en) * 1999-10-13 2010-02-23 Sequenom, Inc. Methods for generating databases and databases for identifying polymorphic genetic markers
WO2001066763A2 (en) * 2000-03-07 2001-09-13 Millennium Pharmaceuticals, Inc. 23436, a human ubiquitin protease family member and uses thereof
US7070947B2 (en) * 2000-02-29 2006-07-04 Millennium Pharmaceuticals, Inc. Human protein kinase, phosphatase, and protease family members and uses thereof
PL366134A1 (en) 2000-06-15 2005-01-24 Smithkline Beecham Corporation Method for preparing a physiologically active il-18 polypeptide
EP1294905A1 (de) * 2000-06-26 2003-03-26 SmithKline Beecham Biologics SA Triple-fusionsprotein von ubiquitin, die zwischen thioredoxin und ein polypeptid von interesse fusioniert ist
US7122332B2 (en) * 2001-03-29 2006-10-17 Rigel Pharmaceuticals, Inc. Modulators of leukocyte activation, compositions and methods of use
AU2003298733B2 (en) * 2002-11-27 2009-06-18 Agena Bioscience, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
EP1618216A2 (de) * 2003-04-25 2006-01-25 Sequenom, Inc. Auf fragmentierung beruhende verfahren und systeme zur de-novo-sequenzierung
WO2005024068A2 (en) * 2003-09-05 2005-03-17 Sequenom, Inc. Allele-specific sequence variation analysis
CA2561381C (en) 2004-03-26 2015-05-12 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
JP2008512129A (ja) * 2004-09-10 2008-04-24 セクエノム，インコーポレイティド 核酸の広範囲配列分析法
WO2006073320A1 (en) * 2005-01-10 2006-07-13 Instytut Biotechnologii Antybiotykow A ubp1 protease mutant,and its coding sequence, their applications and heterogonous protein expression system
US8173792B2 (en) 2007-02-09 2012-05-08 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells using synthetic small molecules
US20090006002A1 (en) * 2007-04-13 2009-01-01 Sequenom, Inc. Comparative sequence analysis processes and systems
US8530636B2 (en) 2008-05-07 2013-09-10 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells in vivo using synthetic small molecules
CA3092860A1 (en) * 2018-03-06 2019-09-12 Pepvax, Inc. Nucleic acid molecules and methods of using the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE247708T1 (de) * 1986-10-02 2003-09-15 Massachusetts Inst Technology Verfahren zur regulierung der metabolischen stabilität von proteinen
US5108919A (en) * 1988-06-24 1992-04-28 Genentech, Inc. Dna sequences encoding yeast ubiquitin hydrolase

Also Published As

Publication number Publication date
JPH06500006A (ja) 1994-01-06
EP0531404B1 (de) 1995-04-19
WO1991017245A1 (en) 1991-11-14
DE69109109D1 (de) 1995-05-24
EP0531404A1 (de) 1993-03-17
US5391490A (en) 1995-02-21
DK0531404T3 (da) 1995-09-18
DE69109109T2 (de) 1995-09-14
CA2079229C (en) 1999-09-14
ES2071315T3 (es) 1995-06-16
CA2079229A1 (en) 1991-11-10
JP3041644B2 (ja) 2000-05-15

Similar Documents

Publication Publication Date Title
ATE121454T1 (de) Ubiquitinspezifische protease.
Carter et al. Engineering subtilisin BPN′ for site‐specific proteolysis
DE3584260D1 (de) Die synthese eines proteins mittels eines identifizierungspeptids.
Ugai et al. Purification and characterization of the 26S proteasome complex catalyzing ATP-dependent breakdown of ubiquitin-ligated prot from rat liver
NZ232742A (en) Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced
ATE272109T1 (de) Rekombinante collagenase typ i aus clostridium histolyticum und ihre verwendung zur isolierung von zellen und zellverbänden
AU6132294A (en) Fusion proteins including a cleavage site recognized by a plant virus protease
WO1996000503B1 (en) Novel peptides
BR9813608A (pt) Polipeptìdeo isolado, sequências de ácido nucleico isolado e mutante, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, processos para produzir uma sequência de ácido nucleico mutante, para produzir um polipeptìdeo, para produzir um mutante de uma célula originária e para produzir uma proteìna, e, célula mutante
ZA8860B (en) Pancreatic secretory trypsin inhibitor and variants thereof produced by a recombinant host,process,expression vector and recombinant host therefor and pharmaceutical use thereof
EP0528686B8 (de) Verfahren zur Herstellung von Peptiden
EP0195680A3 (de) Proteinsynthese mit einem Identifizierungspeptid
ATE317443T1 (de) N-terminal verlängerte proteine exprimiert in hefe
KR940009329A (ko) 딕티오스텔륨 디펩티딜아미노펩티다아제
IL90947A (en) Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production
Sokolovsky et al. Primary structure of cytochrome c from the camel, Camelus dromedarius
Varshavsky et al. Ubiquitin-specific protease
Ohkubo et al. Molecular cloning and immunohistochemical localization of rat dipeptidyl peptidase III
KR860008282A (ko) 재조합 사람 레닌의 제조방법
Harris et al. Amino acid sequences in penicillo-pepsin: evidence for homology with porcine pepsin and chymosin
ATE309263T1 (de) Reinigungsverfahren auf grundlage der bindung von enzymen and markierte peptide
Paech et al. Unusual ligand binding at the active site domain of an engineered mutant of subtilisin BL
WO2001047958A3 (en) Kunitz domain polypeptide zkun10
Pal et al. Crystallization of an enzyme‐inhibitor complex: Proteinase K with its protein inhibitor, PK13
TH17635A (th) อะมิโนเพพติเดสชนิดใหม่ กระบวนการเตรียมอะมิโนเพพติเดสและโปรตีนจากมัน

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee