ATE309263T1 - Reinigungsverfahren auf grundlage der bindung von enzymen and markierte peptide - Google Patents

Reinigungsverfahren auf grundlage der bindung von enzymen and markierte peptide

Info

Publication number
ATE309263T1
ATE309263T1 AT02015128T AT02015128T ATE309263T1 AT E309263 T1 ATE309263 T1 AT E309263T1 AT 02015128 T AT02015128 T AT 02015128T AT 02015128 T AT02015128 T AT 02015128T AT E309263 T1 ATE309263 T1 AT E309263T1
Authority
AT
Austria
Prior art keywords
protein
identification peptide
peptide
functional protein
sequence
Prior art date
Application number
AT02015128T
Other languages
English (en)
Inventor
Francis Edward Dwulet
Neil Gwyn Balgobin
Robert Crane Mccarthy
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE309263T1 publication Critical patent/ATE309263T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/36Extraction; Separation; Purification by a combination of two or more processes of different types
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/8107Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811Serine protease (E.C. 3.4.21) inhibitors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/8107Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114Kunitz type inhibitors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/8107Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114Kunitz type inhibitors
    • C07K14/8117Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/8107Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8135Kazal type inhibitors, e.g. pancreatic secretory inhibitor, ovomucoid
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT02015128T 2001-07-10 2002-07-06 Reinigungsverfahren auf grundlage der bindung von enzymen and markierte peptide ATE309263T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/901,996 US20030032146A1 (en) 2001-07-10 2001-07-10 Enzyme/tag binding and detection system

Publications (1)

Publication Number Publication Date
ATE309263T1 true ATE309263T1 (de) 2005-11-15

Family

ID=25415160

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02015128T ATE309263T1 (de) 2001-07-10 2002-07-06 Reinigungsverfahren auf grundlage der bindung von enzymen and markierte peptide

Country Status (7)

Country Link
US (3) US20030032146A1 (de)
EP (1) EP1281716B1 (de)
JP (1) JP2003093080A (de)
AT (1) ATE309263T1 (de)
CA (1) CA2390522A1 (de)
DE (1) DE60207157T2 (de)
ES (1) ES2252363T3 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003514383A (ja) 1999-11-17 2003-04-15 ロシュ ダイアグノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング 磁性ガラス粒子、それらの製造方法、及びそれらの使用
US6830917B2 (en) 2001-12-10 2004-12-14 Baxter Healthcare S.A. Method of isolation and purification of trypsin from pronase protease and use thereof
CN114040923B (zh) * 2019-04-05 2024-11-26 韩国窑业技术院 利用肌集钙蛋白标签的蛋白质的表达及纯化方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4078853A (en) * 1976-02-25 1978-03-14 Bell Telephone Laboratories, Incorporated Optical communication cable
US4420220A (en) * 1979-06-25 1983-12-13 Bicc Public Limited Company Optical guides
US4979795A (en) * 1989-06-29 1990-12-25 At&T Bell Laboratories Coilable torque-balanced cable and method of manufacture
US5125063A (en) * 1990-11-08 1992-06-23 At&T Bell Laboratories Lightweight optical fiber cable
PT1489097E (pt) * 1994-01-11 2012-01-04 Dyax Corp Inibidores de plasmina humana derivados de domínios kunitz e ácidos nucleicos codificando os mesmos
US6326155B1 (en) * 1995-03-20 2001-12-04 Dyax Corp. Engineering affinity ligands for macromolecules
US5630003A (en) * 1995-11-30 1997-05-13 Lucent Technologies Inc. Loose tube fiber optic cable
US5668912A (en) * 1996-02-07 1997-09-16 Alcatel Na Cable Systems, Inc. Rectangular optical fiber cable
US6380354B1 (en) * 1998-09-03 2002-04-30 Zymogenetics, Inc. Kunitz domain polypeptide zkun6
US6314224B1 (en) * 1999-06-18 2001-11-06 Alcatel Thick-walled cable jacket with non-circular cavity cross section
US6728451B2 (en) * 1999-09-21 2004-04-27 Tyco Telecommunications (Us) Inc. Optical fiber holding structure and method of making same
US6714708B2 (en) * 2001-03-30 2004-03-30 Corning Cable Systems Llc Fiber optic with high strength component
US6618526B2 (en) * 2001-09-27 2003-09-09 Corning Cable Systems Llc Fiber optic cables
US6749446B2 (en) * 2001-10-10 2004-06-15 Alcatel Optical fiber cable with cushion members protecting optical fiber ribbon stack
JP3596511B2 (ja) * 2001-10-18 2004-12-02 住友電気工業株式会社 光ファイバケーブル
US6847768B2 (en) * 2002-09-06 2005-01-25 Corning Cable Systems Llc Optical fiber tube assembly having a plug

Also Published As

Publication number Publication date
EP1281716B1 (de) 2005-11-09
EP1281716A3 (de) 2004-04-07
CA2390522A1 (en) 2003-01-10
US20050214900A1 (en) 2005-09-29
EP1281716A2 (de) 2003-02-05
DE60207157D1 (de) 2005-12-15
US20040110255A1 (en) 2004-06-10
DE60207157T2 (de) 2006-07-13
US20030032146A1 (en) 2003-02-13
JP2003093080A (ja) 2003-04-02
ES2252363T3 (es) 2006-05-16

Similar Documents

Publication Publication Date Title
CA1195627A (en) Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
ATE421977T1 (de) Reinigung von peptiden unter verwendung von metall-ion-affinitätschromatographie
Konigsberg The structure of human hemoglobin. VII. The digestion of the beta chain of human hemoglobin with pepsin
ATE160587T1 (de) Expression rekombinanter polypeptide mit verbesserter möglichkeit zur reinigung
DE3584260D1 (de) Die synthese eines proteins mittels eines identifizierungspeptids.
DK0531404T3 (da) Ubiquitin-specifik protease
ATE123063T1 (de) Peptide zur diagnose von htlv-iii-antikörpern, deren herstellung und verwendung.
CA2443365A1 (en) Methods for the recombinant production of antifusogenic peptides
DE69841939D1 (de) Direkte expression von peptiden ins kulturmedium
CA2273196A1 (en) Isolated mammalian monocyte cell genes; related reagents
ATE420186T1 (de) Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen
DK1616878T3 (da) Udskilte og membranspændende polypeptider samt nukleinsyrer kodende for disse
KR870001311A (ko) 단백질 및 폴리펩티드의 제조 방법
James et al. Application of high-performance liquid chromatographic peptide purification to protein microsequencing by solid-phase Edman degradation
Potts Jr et al. Structural basis of biological and immunological activity of parathyroid hormone.
ATE309263T1 (de) Reinigungsverfahren auf grundlage der bindung von enzymen and markierte peptide
ATE317443T1 (de) N-terminal verlängerte proteine exprimiert in hefe
EP0195680A3 (de) Proteinsynthese mit einem Identifizierungspeptid
WO1999002708A8 (en) Fusion proteins comprising sequences derived from bovine if1 atpase inhibitor protein
WO1998029540A3 (de) REKOMBINANTE FUSIONSPROTEINE AUF DER BASIS RIBOSOMEN-INAKTIVIERENDER PROTEINE DER MISTEL $i(VISCUM ALBUM)
ES2072422T3 (es) Procedimiento para la produccion de peptidos amidados en alfa en el terminal c.
KR960705936A (ko) 돼지 췌장의 카복시펩티다제 b를 암호화하는 dna 서열(dna sequences encoding porcine pancreatic carboxypeptidase b)
KR860008282A (ko) 재조합 사람 레닌의 제조방법
DE60033583D1 (de) Eine mycobacterium tuberculosis kodierende sequenz zur expression von heterologen proteinen
DK0726951T3 (da) Fremstilling og anvendelse af polypeptider påhæftet tag under anvendelse af bindende molekyler

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1281716

Country of ref document: EP

REN Ceased due to non-payment of the annual fee