ATE121778T1 - Expressions- und ausscheidungsvektoren für hirudin mittels transformierter hefe. - Google Patents

Expressions- und ausscheidungsvektoren für hirudin mittels transformierter hefe.

Info

Publication number: ATE121778T1
Authority: AT; Austria
Prior art keywords: hirudin; expression; transformed yeast; excretion; vectors
Prior art date: 1985-05-02

Application number

AT86400961T

Other languages

English (en)

Inventor

Gerard Loison

Paul Tolstoshev

Street

Yves Lemoine

Jean-Pierre Lecocq

Original Assignee

Transgene Sa

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-05-02

Filing date

1986-05-02

Publication date

1995-05-15

1986-05-02 Application filed by Transgene Sa filed Critical Transgene Sa

1995-05-15 Application granted granted Critical

1995-05-15 Publication of ATE121778T1 publication Critical patent/ATE121778T1/de

Links

240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 3
102000007625 Hirudins Human genes 0.000 title 1
108010007267 Hirudins Proteins 0.000 title 1
230000029142 excretion Effects 0.000 title 1
WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 title 1
229940006607 hirudin Drugs 0.000 title 1
239000013598 vector Substances 0.000 title 1
108090000623 proteins and genes Proteins 0.000 abstract 3
108020004414 DNA Proteins 0.000 abstract 1
108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
230000035897 transcription Effects 0.000 abstract 1
238000013518 transcription Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Mycology (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Biochemistry (AREA)
Biophysics (AREA)
Molecular Biology (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
Medicinal Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Zoology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Animal Behavior & Ethology (AREA)
Pharmacology & Pharmacy (AREA)
Tropical Medicine & Parasitology (AREA)
Public Health (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Veterinary Medicine (AREA)
Hematology (AREA)
Diabetes (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Instructional Devices (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Yarns And Mechanical Finishing Of Yarns Or Ropes (AREA)

AT86400961T 1985-05-02 1986-05-02 Expressions- und ausscheidungsvektoren für hirudin mittels transformierter hefe. ATE121778T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
FR8506672A FR2593518B1 (fr)	1985-05-02	1985-05-02	Vecteurs d'expression et de secretion de l'hirudine par les levures transformees

Publications (1)

Publication Number	Publication Date
ATE121778T1 true ATE121778T1 (de)	1995-05-15

Family

ID=9318888

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT86400961T ATE121778T1 (de)	1985-05-02	1986-05-02	Expressions- und ausscheidungsvektoren für hirudin mittels transformierter hefe.

Country Status (22)

Country	Link
EP (1)	EP0200655B1 (de)
JP (2)	JP2580141B2 (de)
KR (1)	KR870700234A (de)
AT (1)	ATE121778T1 (de)
AU (1)	AU600667B2 (de)
BG (1)	BG49717A3 (de)
CA (1)	CA1341501C (de)
CZ (1)	CZ282928B6 (de)
DE (1)	DE3650306T2 (de)
DK (1)	DK174837B1 (de)
ES (1)	ES8703930A1 (de)
FI (1)	FI104635B (de)
FR (2)	FR2593518B1 (de)
HU (1)	HU202919B (de)
IE (1)	IE67136B1 (de)
MC (1)	MC1785A1 (de)
NO (1)	NO302375B1 (de)
PL (1)	PL155074B1 (de)
PT (1)	PT82490B (de)
RU (1)	RU1774950C (de)
WO (1)	WO1986006406A1 (de)
ZA (1)	ZA863261B (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3738541A1 (de) *	1987-11-13	1989-05-24	Hoechst Ag	Verfahren zur isolierung und reinigung von hirudin
FR2593518B1 (fr) *	1985-05-02	1989-09-08	Transgene Sa	Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
MY101203A (en) *	1985-12-12	1991-08-17	Ucp Gen Pharma Ag	Production of thrombin iinhibitors.
GB8530631D0 (en)	1985-12-12	1986-01-22	Ciba Geigy Ag	Thrombin inhibitors
FR2607516B2 (fr) *	1986-12-01	1989-12-22	Transgene Sa	Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu
MC1834A1 (fr) *	1986-07-10	1988-06-03	Transgene Sa	Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
FR2607517B2 (fr) *	1986-12-01	1989-12-22	Transgene Sa	Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
FR2611723B1 (fr) *	1987-02-27	1989-09-08	Transgene Sa	Vecteur d'expression d'adn recombinant codant pour l'hirudine, cultures de cellules de mammiferes infectees ou transformees, procede de preparation de l'hirudine et proteine obtenue
FI896261A7 (fi) *	1987-06-24	1989-12-22	Novo Nordisk As	Menetelmä proteiinin tai polypeptidin valmistamiseksi, polypeptidiä ko odaava DNA-sekvenssi, DNA-sekvenssin sisältämä mikro-organismi sekä po lypeptidi ja sen käyttö farmaseuttisena valmisteena
US5256559A (en) *	1988-03-04	1993-10-26	Biogen, Inc.	Methods and compositions for inhibiting platelet aggregation
DE3819079A1 (de) *	1988-06-04	1989-12-07	Hoechst Ag	Hirudin-derivate mit verzoegerter wirkung
AU633020B2 (en) *	1988-07-23	1993-01-21	Novozymes Delta Limited	New secretory leader sequences
DE3835815A1 (de) *	1988-10-21	1990-04-26	Hoechst Ag	Neue isohirudine
CA2000887A1 (en) *	1988-11-01	1990-05-01	Cecilia S.L. Ku	Thromboresistant materials and methods for making same
US5053453A (en) *	1988-11-01	1991-10-01	Baxter International Inc.	Thromboresistant materials and methods for making same
FR2646437B1 (fr) *	1989-04-28	1991-08-30	Transgene Sa	Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
DE4009268A1 (de) *	1990-03-22	1991-09-26	Consortium Elektrochem Ind	Sekretion von hirudinderivaten
US6514730B1 (en)	1991-03-21	2003-02-04	Consortium für elektrochemische Industrie GmbH	Secretion of hirudin derivatives
US5837808A (en) *	1991-08-20	1998-11-17	Baxter International Inc.	Analogs of hirudin
DE4140381A1 (de) *	1991-12-07	1993-06-09	Hoechst Ag, 6230 Frankfurt, De	Neue synthetische isohirudine mit verbesserter stabilitaet
EP1031628A1 (de) *	1992-09-04	2000-08-30	Novartis AG	Verfahren zur Herstellung von Proteaseinhibitoren
US6500645B1 (en)	1994-06-17	2002-12-31	Novo Nordisk A/S	N-terminally extended proteins expressed in yeast
IL114160A (en) *	1994-06-17	2006-12-31	Novo Nordisk As	Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast
DE19529997C1 (de) *	1995-08-16	1997-04-10	Hoechst Ag	Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
DE19543737A1 (de) *	1995-11-24	1997-05-28	Hoechst Ag	Verfahren zur Ultrafiltration von Peptide oder Proteine enthaltender biologischer Matrices
DE19544233A1 (de)	1995-11-28	1997-06-05	Hoechst Ag	Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
ZA9610456B (en) *	1995-12-20	1997-06-20	Novo Nordisk As	N-terminally extended proteins expressed in yeast
EP0787569B1 (de)	1996-01-31	2002-10-02	Sumitomo Bakelite Company Limited	Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung
US6265204B1 (en)	1997-01-17	2001-07-24	Genencor International, Inc.	DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
US7202059B2 (en)	2001-02-20	2007-04-10	Sanofi-Aventis Deutschland Gmbh	Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en)	2001-02-20	2009-12-29	Sanofi-Aventis Deutschland Gmbh	Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
DE10149678A1 (de)	2001-10-09	2009-03-12	Novel Science International Gmbh	Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
DE10301255A1 (de)	2003-01-15	2004-07-29	Cpi Creative Pharma International Gmbh	Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
US7795205B2 (en)	2004-04-12	2010-09-14	Canyon Pharmaceuticals, Inc.	Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3382547D1 (de) *	1983-01-12	1992-05-27	Chiron Corp	Sekretorische expression in eukaryoten.
AU572125B2 (en) *	1983-03-17	1988-05-05	Mabco Limited	Monoclonal antibodies with specificity for crosslinked fibrin and their diagnotic uses
WO1984004330A1 (en) *	1983-04-22	1984-11-08	Amgen	Secretion of exogenous polypeptides from yeast
NZ207926A (en) *	1983-04-25	1988-04-29	Genentech Inc	Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
JPS6041487A (ja) *	1983-04-25	1985-03-05	ジエネンテツク・インコ−ポレイテツド	酵母発現系でのアルフア因子配列の使用
JPS59227899A (ja) *	1983-05-25	1984-12-21	チロン・コ−ポレイシヨン	成長ホルモン放出因子の製造方法
WO1985004418A1 (fr) *	1984-03-27	1985-10-10	Transgene S.A.	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
ATE64956T1 (de) *	1984-06-14	1991-07-15	Ciba Geigy Ag	Verfahren zur herstellung von thrombininhibitoren.
DE3429430A1 (de) *	1984-08-10	1986-02-20	Hoechst Ag, 6230 Frankfurt	Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3445517C2 (de) *	1984-12-13	1993-11-18	Ciba Geigy	Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
FR2593518B1 (fr) *	1985-05-02	1989-09-08	Transgene Sa	Vecteurs d'expression et de secretion de l'hirudine par les levures transformees

1985
- 1985-05-02 FR FR8506672A patent/FR2593518B1/fr not_active Expired
1986
- 1986-04-30 ZA ZA863261A patent/ZA863261B/xx unknown
- 1986-04-30 CA CA000508053A patent/CA1341501C/fr not_active Expired - Fee Related
- 1986-04-30 PT PT82490A patent/PT82490B/pt unknown
- 1986-05-02 PL PL1986259298A patent/PL155074B1/pl unknown
- 1986-05-02 DE DE3650306T patent/DE3650306T2/de not_active Expired - Lifetime
- 1986-05-02 WO PCT/FR1986/000153 patent/WO1986006406A1/fr not_active Ceased
- 1986-05-02 AU AU57787/86A patent/AU600667B2/en not_active Expired
- 1986-05-02 ES ES555121A patent/ES8703930A1/es not_active Expired
- 1986-05-02 JP JP61502537A patent/JP2580141B2/ja not_active Expired - Lifetime
- 1986-05-02 MC MC86FR8600153D patent/MC1785A1/xx unknown
- 1986-05-02 KR KR860700971A patent/KR870700234A/ko not_active Ceased
- 1986-05-02 AT AT86400961T patent/ATE121778T1/de not_active IP Right Cessation
- 1986-05-02 HU HU863185A patent/HU202919B/hu unknown
- 1986-05-02 EP EP86400961A patent/EP0200655B1/de not_active Expired - Lifetime
- 1986-05-02 IE IE118386A patent/IE67136B1/en not_active IP Right Cessation
- 1986-05-04 CZ CS863217A patent/CZ282928B6/cs not_active IP Right Cessation
- 1986-07-10 FR FR868610090A patent/FR2601383B2/fr not_active Expired
- 1986-12-19 BG BG077615A patent/BG49717A3/xx unknown
- 1986-12-23 FI FI865303A patent/FI104635B/fi not_active IP Right Cessation
- 1986-12-30 DK DK198606343A patent/DK174837B1/da not_active IP Right Cessation
- 1986-12-30 NO NO865336A patent/NO302375B1/no not_active IP Right Cessation
- 1986-12-30 RU SU864028760A patent/RU1774950C/ru active
1996
- 1996-03-26 JP JP8070208A patent/JP2779930B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
FR2601383A2 (fr)	1988-01-15
HU202919B (en)	1991-04-29
FI865303A0 (fi)	1986-12-23
WO1986006406A1 (fr)	1986-11-06
PT82490A (fr)	1986-05-01
PL155074B1 (en)	1991-10-31
JPS62502661A (ja)	1987-10-15
PT82490B (pt)	1988-03-03
CZ282928B6 (cs)	1997-11-12
DK174837B1 (da)	2003-12-15
FI865303L (fi)	1986-12-23
FR2593518B1 (fr)	1989-09-08
AU5778786A (en)	1986-11-18
JP2779930B2 (ja)	1998-07-23
RU1774950C (ru)	1992-11-07
HK1006183A1 (en)	1999-02-12
BG49717A3 (bg)	1992-01-15
IE67136B1 (en)	1996-03-06
AU600667B2 (en)	1990-08-23
DE3650306T2 (de)	1995-10-12
KR870700234A (ko)	1987-05-30
DK634386D0 (da)	1986-12-30
DK634386A (da)	1986-12-30
ZA863261B (en)	1986-12-30
FR2601383B2 (fr)	1989-12-22
CA1341501C (fr)	2006-03-14
FI104635B (fi)	2000-03-15
EP0200655B1 (de)	1995-04-26
JPH09103295A (ja)	1997-04-22
DE3650306D1 (de)	1995-06-01
NO865336L (no)	1987-03-02
MC1785A1 (fr)	1987-09-02
ES8703930A1 (es)	1987-03-16
EP0200655A1 (de)	1986-11-05
NO302375B1 (no)	1998-02-23
FR2593518A1 (fr)	1987-07-31
ES555121A0 (es)	1987-03-16
CZ321786A3 (en)	1997-07-16
JP2580141B2 (ja)	1997-02-12
HUT42131A (en)	1987-06-29
IE861183L (en)	1986-11-02

Legal Events

Date	Code	Title
1995-11-15	UEP	Publication of translation of european patent specification
1997-03-15	EEIH	Change in the person of patent owner
2003-06-15	EEIH	Change in the person of patent owner
2006-08-15	EELA	Cancelled due to lapse of time

Publication	Publication Date	Title
DE3650306D1 (de)	1995-06-01	Expressions- und Ausscheidungsvektoren für Hirudin mittels transformierter Hefe.
EP0223840A4 (de)	1987-07-09	Nukleinsäurensonde zur geschlechtsbestimmung vor der geburt.
DK0506825T3 (da)	1999-05-03	Rekombinante ekspressionsvektorer og oprensningsfremgangsmåder til Thermus thermophilus-DNA-polymerase
DK374686A (da)	1987-04-01	Knoglemorphogenetiske midler, fremgangsmaade til fremstilling deraf og dna-sekvenser, nucleinsyresonder og rekombinante udtrykkelsesmedier til anvendelse derved
FI864964A7 (fi)	1987-06-06	Nukleiinihappojaksojen määrittämiseen soveltuvia molekyylejä.
ATE302850T1 (de)	2005-09-15	Apoptose-proteasen 3 und 4, ähnlich dem umwandlungsenzym für interleukin-1beta
NO872757L (no)	1988-01-04	Alkynylaminonukleotider og fremgangsmte for fremstilling av disse.
ATE113960T1 (de)	1994-11-15	Proteingewinnung.
DE3686399D1 (de)	1992-09-17	Herstellung von thrombin-inhibitoren.
BG80504A (bg)	1993-12-24	Функционален блок на днк
FI90667C (fi)	1994-03-10	Menetelmä valmistaa uutta ihmisen tyypin I interferoniproteiinia
ATE264913T1 (de)	2004-05-15	Protease-resistente analoge des thrombomodulin
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