ATE124949T1 - Verfahren zur isolierung von nukleinsäuren. - Google Patents

Verfahren zur isolierung von nukleinsäuren.

Info

Publication number: ATE124949T1
Authority: AT; Austria
Prior art keywords: nucleic acids; isolation; formamide; dimethylformamide; precipitated
Prior art date: 1987-04-24

Application number

AT88106008T

Other languages

German (de)

English (en)

Inventor

Hansruedi Dr Kiefer

Original Assignee

Hoffmann La Roche

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-04-24

Filing date

1988-04-15

Publication date

1995-07-15

1988-04-15 Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche

1995-07-15 Application granted granted Critical

1995-07-15 Publication of ATE124949T1 publication Critical patent/ATE124949T1/de

Links

150000007523 nucleic acids Chemical class 0.000 title abstract 3
102000039446 nucleic acids Human genes 0.000 title abstract 3
108020004707 nucleic acids Proteins 0.000 title abstract 3
238000002955 isolation Methods 0.000 title abstract 2
238000000034 method Methods 0.000 title abstract 2
ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 abstract 3
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 2
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 abstract 2
239000000284 extract Substances 0.000 abstract 1
150000002576 ketones Chemical class 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Crystallography & Structural Chemistry (AREA)
Plant Pathology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Analytical Chemistry (AREA)
Saccharide Compounds (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

AT88106008T 1987-04-24 1988-04-15 Verfahren zur isolierung von nukleinsäuren. ATE124949T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CH157387		1987-04-24

Publications (1)

Publication Number	Publication Date
ATE124949T1 true ATE124949T1 (de)	1995-07-15

Family

ID=4213584

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT88106008T ATE124949T1 (de)	1987-04-24	1988-04-15	Verfahren zur isolierung von nukleinsäuren.

Country Status (8)

Country	Link
US (1)	US4946952A (fr)
EP (1)	EP0287961B1 (fr)
JP (1)	JP2773835B2 (fr)
AT (1)	ATE124949T1 (fr)
CA (1)	CA1297431C (fr)
DE (1)	DE3854137D1 (fr)
DK (1)	DK193788A (fr)
NO (1)	NO170891C (fr)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0340609A3 (fr) *	1988-05-06	1991-07-31	F. Hoffmann-La Roche Ag	Appareil pour l'isolation des acides nucléiques
CA2067711C (fr) *	1991-05-03	2000-08-08	Daniel Lee Woodard	Purification par extraction en phase solide de l'adn
US5329000A (en) *	1991-10-31	1994-07-12	Becton, Dickinson And Company	Purification of DNA with silicon tetrahydrazide
ATE212848T1 (de) *	1993-05-24	2002-02-15	Np Predpr Sfarmeks	Niedermolekulare dna aus störmilch, methode zur gewinnung der dna und ein pharmazeutisches präparat darauf basierend
US5561064A (en) *	1994-02-01	1996-10-01	Vical Incorporated	Production of pharmaceutical-grade plasmid DNA
US20030033617A1 (en) *	1996-04-10	2003-02-13	Gyula Hadlaczky	Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6025155A (en) *	1996-04-10	2000-02-15	Chromos Molecular Systems, Inc.	Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6077697A (en) *	1996-04-10	2000-06-20	Chromos Molecular Systems, Inc.	Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US20020160970A1 (en) *	1996-04-10	2002-10-31	Gyula Hadlaczky	Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US5973138A (en)	1998-10-30	1999-10-26	Becton Dickinson And Company	Method for purification and manipulation of nucleic acids using paramagnetic particles
CN101845446B (zh) *	2000-09-28	2016-01-20	生物源食物及科学公司	脂肪酸去饱和酶家族成员fad4、fad5、fad5-2和fad6及它们的应用
EP1334113A4 (fr) *	2000-10-20	2007-08-08	Expression Diagnostics Inc	Evaluation du niveau d'expression leucocytaire
US7026121B1 (en)	2001-06-08	2006-04-11	Expression Diagnostics, Inc.	Methods and compositions for diagnosing and monitoring transplant rejection
US7235358B2 (en)	2001-06-08	2007-06-26	Expression Diagnostics, Inc.	Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en)	2001-06-08	2005-06-14	Expression Diagnostics, Inc.	Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
EP1359228B1 (fr) *	2002-04-23	2013-11-27	Accenture Global Services Limited	Authentification d'acide nucléique selon la détection de la lumière diffusée
JP4723254B2 (ja) *	2002-12-23	2011-07-13	バイカルインコーポレイテッド	プラスミドｄｎａを精製するための方法
US7601491B2 (en) *	2003-02-06	2009-10-13	Becton, Dickinson And Company	Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US20040157219A1 (en) *	2003-02-06	2004-08-12	Jianrong Lou	Chemical treatment of biological samples for nucleic acid extraction and kits therefor
WO2004094986A2 (fr) *	2003-04-16	2004-11-04	Handylab, Inc.	Systeme et procede de detection electrochimique de composes biologiques
US7892745B2 (en) *	2003-04-24	2011-02-22	Xdx, Inc.	Methods and compositions for diagnosing and monitoring transplant rejection
US20050123954A1 (en) *	2003-09-12	2005-06-09	Biocontrol Systems, Inc.	Methods, compositions, and kits for the concentration and detection of microorganisms
WO2006029184A2 (fr)	2004-09-08	2006-03-16	Expression Diagnostics, Inc.	Genes permettant de diagnostiquer et de surveiller les troubles associes a l'inflammation
WO2008021431A2 (fr)	2006-08-14	2008-02-21	Xdx, Inc.	Méthodes et compositions permettant de diagnostiquer et de surveiller l'état de rejet de greffe et de troubles immunitaires
EP2102367A2 (fr)	2006-11-09	2009-09-23	XDX, Inc.	Procedes pour diagnostiquer et surveiller l'etat d'un lupus erythemateux systemique
EP2045015B1 (fr)	2007-10-01	2011-11-23	Tecan Trading AG	Agencement de micro-cuvettes et son utilisation
CN101878304B (zh) *	2007-11-30	2015-01-07	通用电气医疗集团生物科学公司	用于从单个样品中分离基因组dna、rna和蛋白质的方法
EP2247746A4 (fr) *	2008-01-31	2012-08-01	Amyris Inc	Procédés de surveillance de voies métaboliques
JP2012502632A (ja) *	2008-09-17	2012-02-02	ジーイー・ヘルスケア・バイオサイエンス・コーポレイション	スモールｒｎａの単離法
JP5826752B2 (ja)	2009-09-14	2015-12-02	キアジェンゲイサーズバーグインコーポレイテッド	細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法
AU2011203450B2 (en)	2010-01-04	2016-01-07	Qiagen Gaithersburg, Inc.	Methods, compositions, and kits for recovery of nucleic acids or proteins from fixed tissue samples
US9145580B2 (en) *	2011-04-02	2015-09-29	New England Biolabs, Inc.	Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
US10351899B2 (en)	2013-09-25	2019-07-16	Bio-ID Diagnostics Inc.	Methods for detecting nucleic acid fragments
GB202310075D0 (en) *	2023-06-30	2023-08-16	Life Tech As	Nucleic acid preparation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CH263036A (de) *	1944-08-14	1949-08-15	Szent Gyorgyi Albert Prof Dr	Verfahren zur Extraktion von Nucleotide enthaltendem Material.
GB1170929A (en) *	1965-12-28	1969-11-19	Glaxo Lab Ltd	Antirival Nucleic Acids.
IT1187833B (it) *	1985-12-12	1987-12-23	Farmigea Spa	Procedimento per l ottenimento di polidesossiribonucleotidi non informazionali sostanzialmente puri e dotati di attivita biologiche e prodotto relativo

1988
- 1988-03-30 CA CA000562889A patent/CA1297431C/fr not_active Expired - Lifetime
- 1988-04-01 US US07/175,885 patent/US4946952A/en not_active Expired - Lifetime
- 1988-04-08 DK DK193788A patent/DK193788A/da not_active Application Discontinuation
- 1988-04-15 DE DE3854137T patent/DE3854137D1/de not_active Expired - Fee Related
- 1988-04-15 EP EP88106008A patent/EP0287961B1/fr not_active Expired - Lifetime
- 1988-04-15 AT AT88106008T patent/ATE124949T1/de not_active IP Right Cessation
- 1988-04-22 NO NO881782A patent/NO170891C/no unknown
- 1988-04-22 JP JP63100014A patent/JP2773835B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
JP2773835B2 (ja)	1998-07-09
NO881782D0 (no)	1988-04-22
DK193788A (da)	1988-10-25
DK193788D0 (da)	1988-04-08
NO881782L (no)	1988-10-25
NO170891C (no)	1992-12-23
EP0287961B1 (fr)	1995-07-12
NO170891B (no)	1992-09-14
JPS63280093A (ja)	1988-11-17
CA1297431C (fr)	1992-03-17
EP0287961A3 (en)	1990-01-10
US4946952A (en)	1990-08-07
EP0287961A2 (fr)	1988-10-26
DE3854137D1 (de)	1995-08-17

Legal Events

Date	Code	Title	Description
2000-01-15	REN	Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
ATE124949T1 (de)	1995-07-15	Verfahren zur isolierung von nukleinsäuren.
DE3873842D1 (de)	1992-09-24	Verfahren zur gen-amplifikation.
DE3777417D1 (de)	1992-04-16	Verfahren zur kryokonservierung von herzklappen.
DE3574931D1 (de)	1990-02-01	Verfahren und anlage zur herstellung von sterilem wasser und sterilen waessrigen loesungen.
DE3874019D1 (de)	1992-10-01	Verfahren zur konservierung von transplantierbaren, in vitro kultivierten epithelgeweben.
DE3863391D1 (de)	1991-08-01	Verfahren zur heliumanreicherung.
DE3787515D1 (de)	1993-10-28	Verfahren zur On-Line-Vorausberechnung und -Kontrolle von alkoholischen Gärungen.
DE3873094D1 (de)	1992-08-27	Verfahren zur aufbereitung von gas.
DE3774897D1 (de)	1992-01-16	Behandelte sojabohnen mit reduziertem phosphor- und kaliumgehalt, verfahren zur zubereitung derselben und damit erhaltene sojabohnen-nahrungsmittel.
DE68914645D1 (de)	1994-05-19	Verfahren zur mikrobiologischen reinigung von wasser.
DE69014109D1 (de)	1994-12-15	Verfahren zur bräunung mit mikrowellen und so hergestelltes produkt.
DE3888443D1 (de)	1994-04-21	Verfahren zur Herstellung von Dihydrochinacridonen, Chinacridonen und Chinacridonchinonen.
FI873477A7 (fi)	1988-02-13	Muovilla päällystetty vuota tai kangas ja menetelmä sen valmistamiseksi.
DE3676892D1 (de)	1991-02-21	Verfahren zur schlammentwaesserung mit biopolymeren.
DE3881091D1 (de)	1993-06-24	Verfahren zur herstellung von phosphatidsaeurederivaten.
DE69105811D1 (de)	1995-01-26	Verfahren zur Extraktion von bestimmten Nukleinsäurefragmenten.
DE3853253D1 (de)	1995-04-13	Verfahren und System zur Sequenzsteuerung.
DE3854096D1 (de)	1995-08-10	Verfahren zur Isolierung von Basalmembranproteinen aus menschlichen und tierischen Geweben.
DE3884113D1 (de)	1993-10-21	Verfahren zur Herstellung von Pfropfpolymerisaten.
DE3871238D1 (de)	1992-06-25	Verfahren zur koproduktion von anilinen und oxamiden.
DE58909276D1 (de)	1995-07-13	Verfahren zur Übertragung von Farbstoffen.
DE3885965D1 (de)	1994-01-13	Verfahren zur Trockenkationisierung von Galaktomannanen (II).
DE3579380D1 (de)	1990-10-04	Verfahren zur elektrischen pruefung von mikroverdrahtungen mit hilfe von korpuskularsonden.
DE59101263D1 (de)	1994-05-05	Verfahren und Apparat zur Aufwärmung und mehrstufigen Entgasung von Wasser.
DE3887350D1 (de)	1994-03-03	Verfahren und system zur objektiven kennzeichnung von farbe.