ATE135606T1 - Fraktionierung nukleider säuren mit hilfe von kapillarer elektrophorese in gegenmigration - Google Patents

Fraktionierung nukleider säuren mit hilfe von kapillarer elektrophorese in gegenmigration

Info

Publication number
ATE135606T1
ATE135606T1 AT90912127T AT90912127T ATE135606T1 AT E135606 T1 ATE135606 T1 AT E135606T1 AT 90912127 T AT90912127 T AT 90912127T AT 90912127 T AT90912127 T AT 90912127T AT E135606 T1 ATE135606 T1 AT E135606T1
Authority
AT
Austria
Prior art keywords
fractionation
countermigration
nucleic acids
capillary electrophoresis
fragments
Prior art date
Application number
AT90912127T
Other languages
English (en)
Inventor
Allan Michael Chin
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of ATE135606T1 publication Critical patent/ATE135606T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44765—Apparatus specially adapted therefor of the counter-flow type

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
AT90912127T 1989-08-07 1990-08-06 Fraktionierung nukleider säuren mit hilfe von kapillarer elektrophorese in gegenmigration ATE135606T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/390,631 US5096554A (en) 1989-08-07 1989-08-07 Nucleic acid fractionation by counter-migration capillary electrophoresis

Publications (1)

Publication Number Publication Date
ATE135606T1 true ATE135606T1 (de) 1996-04-15

Family

ID=23543283

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90912127T ATE135606T1 (de) 1989-08-07 1990-08-06 Fraktionierung nukleider säuren mit hilfe von kapillarer elektrophorese in gegenmigration

Country Status (7)

Country Link
US (2) US5096554A (de)
EP (1) EP0486559B1 (de)
JP (1) JPH0797101B2 (de)
AT (1) ATE135606T1 (de)
DE (1) DE69026090T2 (de)
DK (1) DK0486559T3 (de)
WO (1) WO1991002244A1 (de)

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EP0720658A1 (de) * 1993-09-23 1996-07-10 E.I. Du Pont De Nemours And Company Elektrophoretisches verfahren zur isolierung und trennung von mikroorganismen
US5391274A (en) * 1993-10-18 1995-02-21 Beckman Instruments, Inc. Methods for controlling electroosmotic flow in coated capillary electrophoresis columns
US5891313A (en) 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
US5423966A (en) * 1994-01-25 1995-06-13 Perkin-Elmer Corporation On line ion contaminant removal apparatus and method for capillary electrophoresis
US6001229A (en) * 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5573651A (en) * 1995-04-17 1996-11-12 The Dow Chemical Company Apparatus and method for flow injection analysis
US6017496A (en) 1995-06-07 2000-01-25 Irori Matrices with memories and uses thereof
US6329139B1 (en) 1995-04-25 2001-12-11 Discovery Partners International Automated sorting system for matrices with memory
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US5964995A (en) 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US5948227A (en) * 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
US6537432B1 (en) 1998-02-24 2003-03-25 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
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US6818112B2 (en) 1999-04-20 2004-11-16 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
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Also Published As

Publication number Publication date
DK0486559T3 (da) 1996-07-22
JPH0797101B2 (ja) 1995-10-18
US5096554A (en) 1992-03-17
WO1991002244A1 (en) 1991-02-21
US5110424A (en) 1992-05-05
EP0486559B1 (de) 1996-03-20
EP0486559A4 (en) 1993-03-17
DE69026090T2 (de) 1996-11-14
EP0486559A1 (de) 1992-05-27
JPH04507001A (ja) 1992-12-03
DE69026090D1 (de) 1996-04-25

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee