ATE136932T1 - Erhöhte produktion von thermus aquaticus dna polymerase in e. coli - Google Patents

Erhöhte produktion von thermus aquaticus dna polymerase in e. coli

Info

Publication number
ATE136932T1
ATE136932T1 AT91202731T AT91202731T ATE136932T1 AT E136932 T1 ATE136932 T1 AT E136932T1 AT 91202731 T AT91202731 T AT 91202731T AT 91202731 T AT91202731 T AT 91202731T AT E136932 T1 ATE136932 T1 AT E136932T1
Authority
AT
Austria
Prior art keywords
coli
dna polymerase
thermus aquaticus
increased production
aquaticus dna
Prior art date
Application number
AT91202731T
Other languages
English (en)
Inventor
Mark A Sullivan
Original Assignee
Johnson & Johnson Clin Diag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Clin Diag filed Critical Johnson & Johnson Clin Diag
Application granted granted Critical
Publication of ATE136932T1 publication Critical patent/ATE136932T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AT91202731T 1990-10-26 1991-10-22 Erhöhte produktion von thermus aquaticus dna polymerase in e. coli ATE136932T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/602,848 US6083686A (en) 1990-10-26 1990-10-26 Increased production of Thermus aquaticus DNA polymerase in E. coli

Publications (1)

Publication Number Publication Date
ATE136932T1 true ATE136932T1 (de) 1996-05-15

Family

ID=24413037

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91202731T ATE136932T1 (de) 1990-10-26 1991-10-22 Erhöhte produktion von thermus aquaticus dna polymerase in e. coli

Country Status (15)

Country Link
US (1) US6083686A (de)
EP (1) EP0482714B1 (de)
JP (1) JPH0759195B2 (de)
KR (1) KR940005592B1 (de)
AT (1) ATE136932T1 (de)
AU (1) AU8469091A (de)
CA (1) CA2052827A1 (de)
CS (1) CS324291A3 (de)
DE (1) DE69118809T2 (de)
DK (1) DK0482714T3 (de)
ES (1) ES2086479T3 (de)
HU (1) HUT62334A (de)
IL (1) IL99852A0 (de)
NO (1) NO914191L (de)
SG (1) SG52453A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US6872816B1 (en) 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5888780A (en) * 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
US6673616B1 (en) 1992-12-07 2004-01-06 Third Wave Technologies, Inc. Methods and compositions for characterizing nucleic acids
US5719028A (en) * 1992-12-07 1998-02-17 Third Wave Technologies Inc. Cleavase fragment length polymorphism
US6372424B1 (en) 1995-08-30 2002-04-16 Third Wave Technologies, Inc Rapid detection and identification of pathogens
US5843654A (en) * 1992-12-07 1998-12-01 Third Wave Technologies, Inc. Rapid detection of mutations in the p53 gene
US5614402A (en) * 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
US7785852B2 (en) 1992-12-07 2010-08-31 Third Wave Technologies, Inc. Cleavage of nucleic acids
US6100078A (en) * 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5985557A (en) * 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US7195871B2 (en) 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US7122364B1 (en) 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
ES2375764T3 (es) 1996-11-29 2012-03-06 Third Wave Technologies, Inc. Endonucleasas fen-1, mezclas y procedimientos de escisión.
US6346379B1 (en) * 1997-09-11 2002-02-12 F. Hoffman-La Roche Ag Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
DE19810879A1 (de) * 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
US7148340B2 (en) 2001-11-30 2006-12-12 Applera Corporation Thermus thermophilus nucleic acid polymerases
WO2010062777A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Modified type a dna polymerases
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
KR101948248B1 (ko) * 2011-11-18 2019-02-14 한국생명공학연구원 단백질 발현 미세 조절방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4703004A (en) * 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
US4782137A (en) * 1984-01-24 1988-11-01 Immunex Corporation Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
JP2970811B2 (ja) * 1987-10-30 1999-11-02 オンコゲン ア リミテッド パートナーシップ 原核生物細胞中にポリペプチドを調製するための発現システム
IL88923A (en) * 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification

Also Published As

Publication number Publication date
CA2052827A1 (en) 1992-04-27
NO914191L (no) 1992-04-27
KR940005592B1 (ko) 1994-06-21
CS324291A3 (en) 1992-05-13
US6083686A (en) 2000-07-04
EP0482714A1 (de) 1992-04-29
SG52453A1 (en) 1998-09-28
HUT62334A (en) 1993-04-28
ES2086479T3 (es) 1996-07-01
JPH05130871A (ja) 1993-05-28
KR920008186A (ko) 1992-05-27
DK0482714T3 (da) 1996-05-13
HU913365D0 (en) 1992-01-28
IL99852A0 (en) 1992-08-18
EP0482714B1 (de) 1996-04-17
JPH0759195B2 (ja) 1995-06-28
DE69118809D1 (de) 1996-05-23
DE69118809T2 (de) 1996-09-26
NO914191D0 (no) 1991-10-25
AU8469091A (en) 1992-05-07

Similar Documents

Publication Publication Date Title
DE69118809D1 (de) Erhöhte Produktion von thermus aquaticus DNA Polymerase in E. coli
AU3062989A (en) Purified thermostable enzyme
DE69627698D1 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
DE69133629D1 (de) nssystem zur Bestimmung von genetischen Krankheiten
NO984157L (no) Forandrete termostabile DNA-polymeraser for sekvensbestemmelse
DE69524653D1 (de) Amplifizierung von langen Nukleinsäuresequenzen mit PCR
ES2152923T3 (es) Enzima polimerasa de acido nucleico termoestable purificada, de thermosipho africanus.
CA2283789A1 (en) Dna polymerases having improved labeled nucleotide incorporation properties
BR8704332A (pt) Enzima termoestavel purificada,codificacao de gene de enzima,plasmidio selecionado,bactereofogo,composicao de enzima estavel,processo para amplificar pelo menos uma sequencia especifica de acido nucleico,processo para detectar presenca ou ausencia de pelo menos uma sequencia especifica de acido nucleico,processo para detectar presenca ou ausencia de pelo menos uma variacao de nucleotideos em sequencia em um ou mais acidos nucleicos,processo para clonagem em vetor de clonagem de uma ou mais sequencias especificas de acido nucleico e sequencia amplificada de acido nucleico
ES2100945T3 (es) Transcriptasas reversas de alta temperatura.
ATE147441T1 (de) Verwendung von nuklein-säure analoge in der inhibition von nuklein-säure amplifikation
DE69121975D1 (de) Spezifischer Nachweis von Mycobacterium Tuberculosis
FI923239A0 (fi) Immobilisering av nukleinsyror.
DE69031042D1 (de) Fermentationsprozess zur herstellung von pyrimidin
DE69737024D1 (en) Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius
KR880014106A (ko) 안정한 크레아틴 아미디노 가수분해효소 돌연변이체
Ivanova et al. Phylogenetic relationship of purple bacteria belonging to the family Chromatiaceae.
Esser et al. Replication of deoxyribonucleic acid (DNA) with respect to gene technology

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties