ES2152923T3 - Enzima polimerasa de acido nucleico termoestable purificada, de thermosipho africanus. - Google Patents

Enzima polimerasa de acido nucleico termoestable purificada, de thermosipho africanus.

Info

Publication number
ES2152923T3
ES2152923T3 ES91920000T ES91920000T ES2152923T3 ES 2152923 T3 ES2152923 T3 ES 2152923T3 ES 91920000 T ES91920000 T ES 91920000T ES 91920000 T ES91920000 T ES 91920000T ES 2152923 T3 ES2152923 T3 ES 2152923T3
Authority
ES
Spain
Prior art keywords
pct
nucleic acid
enzyme
thermosipho africanus
purified thermostable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91920000T
Other languages
English (en)
Inventor
David H Gelfand
Frances C Lawyer
Richard D Abramson
I Lawrence Greenfield
Fred L Reichert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2152923T3 publication Critical patent/ES2152923T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Feed For Specific Animals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE PRESENTA UNA ENZIMA PURIFICADA Y TERMOESTABLE DERIVADA DEL "THERMOSIPHO AFRICANUS". LA ENZIMA PRESENTA ACTIVIDAD DE POLIMERASA DE DNA, ACTIVIDAD DE TRANSCRIPTASA INVERSA, Y OPCIONALMENTE ACTIVIDAD DE EXONUCLEASA 5''-> 3'', Y/O 3''-> 5''. LA ENZIMA PUEDE SER NATIVA O RECOMBINANTE, Y PUEDE USARSE CON TRIFOSFATOS NUCLEOSIDOS EN UNA REACCION DE CADENA CICLIDIZADA MEDIANTE LA TEMPERATURA EN LA QUE AL MENOS UNA SECUENCIA DE ACIDO NUCLEICO SE AMPLIFICA A PARTIR DE UNA SECUENCIA EXISTENTE.
ES91920000T 1990-09-28 1991-09-26 Enzima polimerasa de acido nucleico termoestable purificada, de thermosipho africanus. Expired - Lifetime ES2152923T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US59049090A 1990-09-28 1990-09-28

Publications (1)

Publication Number Publication Date
ES2152923T3 true ES2152923T3 (es) 2001-02-16

Family

ID=24362481

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91920000T Expired - Lifetime ES2152923T3 (es) 1990-09-28 1991-09-26 Enzima polimerasa de acido nucleico termoestable purificada, de thermosipho africanus.

Country Status (10)

Country Link
US (1) US5968799A (es)
EP (1) EP0550696B1 (es)
JP (1) JP2582980B2 (es)
AT (1) ATE197718T1 (es)
AU (1) AU658378B2 (es)
CA (1) CA2092317A1 (es)
DE (1) DE69132477T2 (es)
DK (1) DK0550696T3 (es)
ES (1) ES2152923T3 (es)
WO (1) WO1992006202A1 (es)

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US6100078A (en) * 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
US5939257A (en) * 1994-04-18 1999-08-17 Amersham Life Science, Inc. Thermostable alkaline phosphatases
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
WO1995030756A1 (en) * 1994-04-18 1995-11-16 United States Biochemical Corporation Thermostable alkaline phosphatase of thermus thermophilus
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
WO1997009451A1 (en) * 1995-09-08 1997-03-13 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
PT1015576E (pt) * 1997-09-16 2005-09-30 Egea Biosciences Llc Metodo para a sintese quimica completa e montagem de genes e de genomas
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1385950B1 (en) * 2001-01-19 2008-07-02 Centocor, Inc. Computer-directed assembly of a polynucleotide encoding a target polypeptide
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
EP1611254B1 (en) 2003-03-31 2014-09-10 Roche Diagnostics GmbH Compositions and methods for detecting certain flaviviruses, including members of the japanese encephalitis virus serogroup
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
WO2005030995A1 (en) * 2003-09-23 2005-04-07 University Of Missouri Methods of synthesizing polynucleotides using thermostable enzymes
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2006029184A2 (en) 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
EP2102367A2 (en) 2006-11-09 2009-09-23 XDX, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
ES2561885T3 (es) * 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
FI3461807T3 (fi) 2011-06-08 2023-09-07 Life Technologies Corp Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten
ES2569723T3 (es) 2011-12-08 2016-05-12 F. Hoffmann-La Roche Ag Polimerasas de DNA con actividad mejorada
ES2668448T3 (es) 2011-12-08 2018-05-18 F. Hoffmann-La Roche Ag ADN polimerasas con actividad mejorada
CN103998603B (zh) 2011-12-08 2016-05-25 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
CN112760305B (zh) * 2021-01-25 2022-04-29 浙江工业大学 一种栖热腔菌磷酸酶突变体及其应用

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US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
ZA876216B (en) * 1986-08-22 1989-04-26 Cetus Corp Purified thermostable enzyme
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IL88923A (en) * 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
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JP2709311B2 (ja) * 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異

Also Published As

Publication number Publication date
US5968799A (en) 1999-10-19
CA2092317A1 (en) 1992-03-29
DE69132477D1 (en) 2000-12-28
AU8907791A (en) 1992-04-28
ATE197718T1 (de) 2000-12-15
JP2582980B2 (ja) 1997-02-19
EP0550696A1 (en) 1993-07-14
AU658378B2 (en) 1995-04-13
DK0550696T3 (da) 2001-03-19
DE69132477T2 (de) 2001-06-13
WO1992006202A1 (en) 1992-04-16
EP0550696B1 (en) 2000-11-22
JPH06504196A (ja) 1994-05-19

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