ATE138107T1 - Steigerung der polynukleotid hybridisierung - Google Patents
Steigerung der polynukleotid hybridisierungInfo
- Publication number
- ATE138107T1 ATE138107T1 AT92913504T AT92913504T ATE138107T1 AT E138107 T1 ATE138107 T1 AT E138107T1 AT 92913504 T AT92913504 T AT 92913504T AT 92913504 T AT92913504 T AT 92913504T AT E138107 T1 ATE138107 T1 AT E138107T1
- Authority
- AT
- Austria
- Prior art keywords
- pct
- sec
- date
- complementary polynucleotides
- polynucleotide hybridization
- Prior art date
Links
- 102000040430 polynucleotide Human genes 0.000 title abstract 3
- 108091033319 polynucleotide Proteins 0.000 title abstract 3
- 239000002157 polynucleotide Substances 0.000 title abstract 3
- 238000009396 hybridization Methods 0.000 title abstract 2
- 230000000295 complement effect Effects 0.000 abstract 2
- 229920002307 Dextran Polymers 0.000 abstract 1
- 239000002202 Polyethylene glycol Substances 0.000 abstract 1
- 239000004793 Polystyrene Substances 0.000 abstract 1
- 239000004372 Polyvinyl alcohol Substances 0.000 abstract 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 abstract 1
- 239000000872 buffer Substances 0.000 abstract 1
- 125000002091 cationic group Chemical group 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 239000003623 enhancer Substances 0.000 abstract 1
- 238000007901 in situ hybridization Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 229920001223 polyethylene glycol Polymers 0.000 abstract 1
- 229920002223 polystyrene Polymers 0.000 abstract 1
- 229920002451 polyvinyl alcohol Polymers 0.000 abstract 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-N sulfonic acid Chemical compound OS(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-N 0.000 abstract 1
- 229910021653 sulphate ion Inorganic materials 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB919114180A GB9114180D0 (en) | 1991-07-01 | 1991-07-01 | Enhancement of polynucleotide hybridisation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE138107T1 true ATE138107T1 (de) | 1996-06-15 |
Family
ID=10697624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT92913504T ATE138107T1 (de) | 1991-07-01 | 1992-06-30 | Steigerung der polynukleotid hybridisierung |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5512436A (de) |
| EP (1) | EP0610212B1 (de) |
| JP (1) | JP3236293B2 (de) |
| AT (1) | ATE138107T1 (de) |
| DE (1) | DE69210833T2 (de) |
| GB (1) | GB9114180D0 (de) |
| WO (1) | WO1993001311A1 (de) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5853986A (en) * | 1990-04-02 | 1998-12-29 | Becton Dickinson And Company | Chemical promotion of nucleic acid hybridization |
| US6040295A (en) | 1995-01-13 | 2000-03-21 | Genemedicine, Inc. | Formulated nucleic acid compositions and methods of administering the same for gene therapy |
| US6022689A (en) * | 1995-04-07 | 2000-02-08 | University Of New Mexico | Situ hybridization slide processes |
| US5750340A (en) * | 1995-04-07 | 1998-05-12 | University Of New Mexico | In situ hybridization solution and process |
| US6194146B1 (en) | 1997-06-13 | 2001-02-27 | Bioseparations, Inc. | Situ and in vitro hybridization method and buffer |
| US5948617A (en) * | 1997-06-13 | 1999-09-07 | Biospeparations, Inc. | Methods of in situ hybridization |
| AU8327298A (en) * | 1997-07-03 | 1999-01-25 | Id Biomedical Corporation | Methods for accelerating hybridization of nucleic acid molecules |
| US6503709B1 (en) | 1997-07-03 | 2003-01-07 | Id Biomedical Corporation | Methods for rapidly detecting methicillin resistant staphylococci |
| US5985567A (en) * | 1997-08-15 | 1999-11-16 | Beckman Coulter, Inc. | Hybridization detection by pretreating bound single-stranded probes |
| US6203989B1 (en) | 1998-09-30 | 2001-03-20 | Affymetrix, Inc. | Methods and compositions for amplifying detectable signals in specific binding assays |
| AU2002230901B2 (en) | 2000-12-14 | 2006-09-07 | Gen-Probe Incorporated | Method and kit for enhancing the association rates of polynucleotides |
| US6919180B2 (en) * | 2002-03-22 | 2005-07-19 | Sigma Genosys, L.P. | Hybridization rate enhancement for substrate-bound specific nucleic acid-binding agents |
| US20040166520A1 (en) * | 2003-01-03 | 2004-08-26 | Connolly D. Michael | Identifying items with nucleic acid taggants |
| US8076126B2 (en) | 2008-07-18 | 2011-12-13 | Ortho-Clinical Diagnostics, Inc. | Single column immunological test elements |
| CN102257160A (zh) * | 2008-12-18 | 2011-11-23 | 西门子医疗保健诊断公司 | 用于缩短杂交分析中的培养时间的方法和试剂 |
| US10364455B2 (en) * | 2012-09-27 | 2019-07-30 | Bioo Scientific Corporation | Methods and compositions for improving removal of ribosomal RNA from biological samples |
| US9587268B2 (en) | 2014-01-29 | 2017-03-07 | Agilent Technologies Inc. | Fast hybridization for next generation sequencing target enrichment |
| US10704094B1 (en) | 2018-11-14 | 2020-07-07 | Element Biosciences, Inc. | Multipart reagents having increased avidity for polymerase binding |
| EP3947718A4 (de) * | 2019-04-02 | 2022-12-21 | Enumera Molecular, Inc. | Verfahren, systeme und zusammensetzungen zum zählen von nukleinsäuremolekülen |
| US12313627B2 (en) | 2019-05-01 | 2025-05-27 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| CN114836526B (zh) * | 2020-08-31 | 2025-01-10 | 伯科生物科技有限公司 | 一种靶向测序方法及试剂盒 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4302204A (en) * | 1979-07-02 | 1981-11-24 | The Board Of Trustees Of Leland Stanford Junior University | Transfer and detection of nucleic acids |
| US4689294A (en) * | 1984-11-19 | 1987-08-25 | Miles Laboratories, Inc. | Enhancement of hybridization of nucleic acids by anionic polymers |
| DE3687645T2 (de) * | 1986-01-07 | 1993-05-27 | Gen Probe Inc | Verfahren der beschleunigten wiederassoziation der nukleinsaeure. |
| US4886741A (en) * | 1987-12-09 | 1989-12-12 | Microprobe Corporation | Use of volume exclusion agents for the enhancement of in situ hybridization |
-
1991
- 1991-07-01 GB GB919114180A patent/GB9114180D0/en active Pending
-
1992
- 1992-06-30 JP JP50194193A patent/JP3236293B2/ja not_active Expired - Lifetime
- 1992-06-30 DE DE69210833T patent/DE69210833T2/de not_active Expired - Lifetime
- 1992-06-30 WO PCT/EP1992/001479 patent/WO1993001311A1/en not_active Ceased
- 1992-06-30 EP EP92913504A patent/EP0610212B1/de not_active Expired - Lifetime
- 1992-06-30 AT AT92913504T patent/ATE138107T1/de not_active IP Right Cessation
- 1992-06-30 US US08/108,578 patent/US5512436A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0610212A1 (de) | 1994-08-17 |
| GB9114180D0 (en) | 1991-08-21 |
| DE69210833D1 (de) | 1996-06-20 |
| EP0610212B1 (de) | 1996-05-15 |
| WO1993001311A1 (en) | 1993-01-21 |
| JP3236293B2 (ja) | 2001-12-10 |
| US5512436A (en) | 1996-04-30 |
| DE69210833T2 (de) | 1996-10-31 |
| JPH06508992A (ja) | 1994-10-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |