ATE140927T1 - Reinigungsverfahren von transformierendem wachstumsfaktor (tgf) - Google Patents

Reinigungsverfahren von transformierendem wachstumsfaktor (tgf)

Info

Publication number: ATE140927T1
Authority: AT; Austria
Prior art keywords: tgf; growth factor; transforming growth; purification process; sup
Prior art date: 1989-09-11

Application number

AT90914805T

Other languages

English (en)

Inventor

Erno Pungor Jr

Eirik Nestaas

Original Assignee

Berlex Lab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-09-11

Filing date

1990-09-10

Publication date

1996-08-15

1990-09-10 Application filed by Berlex Lab filed Critical Berlex Lab

1996-08-15 Application granted granted Critical

1996-08-15 Publication of ATE140927T1 publication Critical patent/ATE140927T1/de

Links

102000009618 Transforming Growth Factors Human genes 0.000 title 2
108010009583 Transforming Growth Factors Proteins 0.000 title 2
238000000746 purification Methods 0.000 title 1
102000004887 Transforming Growth Factor beta Human genes 0.000 abstract 4
108090001012 Transforming Growth Factor beta Proteins 0.000 abstract 4
ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 abstract 4
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 abstract 1
102000046299 Transforming Growth Factor beta1 Human genes 0.000 abstract 1
101800002279 Transforming growth factor beta-1 Proteins 0.000 abstract 1
238000005277 cation exchange chromatography Methods 0.000 abstract 1
239000003729 cation exchange resin Substances 0.000 abstract 1
239000003636 conditioned culture medium Substances 0.000 abstract 1
230000000694 effects Effects 0.000 abstract 1
230000004151 fermentation Effects 0.000 abstract 1
238000000855 fermentation Methods 0.000 abstract 1
238000004191 hydrophobic interaction chromatography Methods 0.000 abstract 1
239000000203 mixture Substances 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
230000010412 perfusion Effects 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1
108090000623 proteins and genes Proteins 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/495—Transforming growth factor [TGF]

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Genetics & Genomics (AREA)
Medicinal Chemistry (AREA)
Toxicology (AREA)
Biomedical Technology (AREA)
Sustainable Development (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
General Engineering & Computer Science (AREA)
Biophysics (AREA)
Gastroenterology & Hepatology (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Veterinary Medicine (AREA)
Public Health (AREA)
Animal Behavior & Ethology (AREA)
Pharmacology & Pharmacy (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Steroid Compounds (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)

AT90914805T 1989-09-11 1990-09-10 Reinigungsverfahren von transformierendem wachstumsfaktor (tgf) ATE140927T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US07/405,695 US5043431A (en)	1989-09-11	1989-09-11	Method and apparatus for the production of TGF-β

Publications (1)

Publication Number	Publication Date
ATE140927T1 true ATE140927T1 (de)	1996-08-15

Family

ID=23604819

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
AT95119910T ATE231165T1 (de)	1989-09-11	1990-09-10	Verfahren zur herstellung von tgf-beta
AT90914805T ATE140927T1 (de)	1989-09-11	1990-09-10	Reinigungsverfahren von transformierendem wachstumsfaktor (tgf)

Family Applications Before (1)

Application Number	Title	Priority Date	Filing Date
AT95119910T ATE231165T1 (de)	1989-09-11	1990-09-10	Verfahren zur herstellung von tgf-beta

Country Status (10)

Country	Link
US (2)	US5043431A (de)
EP (4)	EP1104807A2 (de)
JP (1)	JPH05500898A (de)
AT (2)	ATE231165T1 (de)
AU (1)	AU6504490A (de)
CA (1)	CA2066576A1 (de)
DE (2)	DE69027995T2 (de)
DK (2)	DK0722951T3 (de)
ES (2)	ES2093031T3 (de)
WO (1)	WO1991004267A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5221734A (en) *	1987-10-01	1993-06-22	Ciba-Geigy Corporation	Process for preparing a polypeptide growth factor for milk
US5043431A (en) *	1989-09-11	1991-08-27	Codon	Method and apparatus for the production of TGF-β
US5206023A (en) *	1991-01-31	1993-04-27	Robert F. Shaw	Method and compositions for the treatment and repair of defects or lesions in cartilage
US5270300A (en) *	1991-09-06	1993-12-14	Robert Francis Shaw	Methods and compositions for the treatment and repair of defects or lesions in cartilage or bone
JP3386498B2 (ja) *	1992-11-06	2003-03-17	森永製菓株式会社	ヒトＴＧＦ−βの精製法
US5331095A (en) *	1993-04-12	1994-07-19	Scios Nova Inc.	Process for purification of basic fibroblast growth factor
US6441152B1 (en) *	1998-12-08	2002-08-27	Boston Probes, Inc.	Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
DE19918409A1 (de) *	1999-04-22	2000-10-26	Forschungszentrum Juelich Gmbh	Bioreaktor umfassend einen Medienablauf und einen Rührer sowie Rührer und Medienablauf
FR2827290B1 (fr) *	2001-07-13	2004-07-09	Pierre Jouan Biotechnologies Sa	Procede d'obtention d'une fraction proteique enrichie en tgf-beta sous forme activee, fraction proteique et applications therapeutiques
US6849394B2 (en) *	2002-02-21	2005-02-01	Minitube Of America	Compositions comprising reproductive cell media and methods for using such compositions
EP1728852A1 (de) *	2005-05-30	2006-12-06	Chiron Behring GmbH & Co. KG	Fermentersystem für Biotechnischen Prozessen
EP2334803A1 (de) *	2008-10-06	2011-06-22	F. Hoffmann-La Roche AG	Schüttelkolbenkultivierung im kleinen massstab
EP3271380B1 (de)	2015-03-17	2021-08-11	ISTITUTO BIOCHIMICO ITALIANO GIOVANNI LORENZINI S.p.A.	Verfahren zur reinigung knochenmorphogenetischer proteine
GB201509193D0 (en)	2015-05-28	2015-07-15	Ge Healthcare Bio Sciences Ab	Method and system for cell cultivation
US9617191B1 (en) *	2016-06-09	2017-04-11	Xianggen Wu	Bioreactor system and method

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5630925A (en) *	1979-08-21	1981-03-28	Sumitomo Chem Co Ltd	Purification of human growth hormone
US4296205A (en) *	1980-02-22	1981-10-20	Verma Dharmvir S	Cell culture and continuous dialysis flask and method
US4427580A (en) *	1982-09-01	1984-01-24	Cornell Research Foundation, Inc.	Method for separation and recovery of proteins and nucleic acids from nucleoproteins using water destructuring salts
US4444760A (en) *	1983-06-17	1984-04-24	Merck & Co., Inc.	Purification and characterization of a protein fibroblast growth factor
US4897464A (en) *	1985-04-17	1990-01-30	President And Fellows Of Harvard College	Purified protein having angiogenic activity and methods of preparation
US4751078A (en) *	1985-07-03	1988-06-14	Nagabhushan Tattanahalli L	Process for the purification of gamma interferon
US4894439A (en) *	1986-05-22	1990-01-16	Cetus Corporation	N-terminal derivatives of tumor necrosis factor purified by microporous PTFE membranes
EP0268561A3 (de) *	1986-11-17	1988-10-26	Sandoz Ag	Herstellung und Verwendung eines T-Zellen-Suppressorfaktors
US4931548A (en) *	1987-01-30	1990-06-05	Techne Corporation	Heterodimer form of transforming growth factor-beta
EP0309111A3 (de) *	1987-09-03	1990-04-18	Invitron Corporation	Verfahren zur Erzielung höherer Ausbeuten an nichtaggregiertem Gewebeplasminogenaktivator aus Zellkulturen
EP0323842A3 (de) *	1988-01-07	1990-04-25	Otsuka Pharmaceutical Co., Ltd.	Methode für die Säuberung von umbildendem Wachstums-Faktor-Beta
EP0338716A3 (de) *	1988-04-21	1990-04-11	Berlex Laboratories, Inc.	Methode und Vorrichtung zur Produktion nichtdegradierter Proteine aus Säugetierzellen
US5043431A (en) *	1989-09-11	1991-08-27	Codon	Method and apparatus for the production of TGF-β

1989
- 1989-09-11 US US07/405,695 patent/US5043431A/en not_active Expired - Fee Related
1990
- 1990-09-10 DK DK95119910T patent/DK0722951T3/da active
- 1990-09-10 ES ES90914805T patent/ES2093031T3/es not_active Expired - Lifetime
- 1990-09-10 JP JP2513740A patent/JPH05500898A/ja active Pending
- 1990-09-10 EP EP00250420A patent/EP1104807A2/de not_active Withdrawn
- 1990-09-10 EP EP95119910A patent/EP0722951B1/de not_active Expired - Lifetime
- 1990-09-10 AU AU65044/90A patent/AU6504490A/en not_active Abandoned
- 1990-09-10 AT AT95119910T patent/ATE231165T1/de not_active IP Right Cessation
- 1990-09-10 ES ES95119910T patent/ES2188632T3/es not_active Expired - Lifetime
- 1990-09-10 DK DK90914805.8T patent/DK0491849T3/da active
- 1990-09-10 EP EP90914805A patent/EP0491849B1/de not_active Expired - Lifetime
- 1990-09-10 DE DE69027995T patent/DE69027995T2/de not_active Expired - Lifetime
- 1990-09-10 EP EP00250419A patent/EP1132469A3/de not_active Withdrawn
- 1990-09-10 WO PCT/US1990/005098 patent/WO1991004267A1/en not_active Ceased
- 1990-09-10 AT AT90914805T patent/ATE140927T1/de not_active IP Right Cessation
- 1990-09-10 DE DE69034037T patent/DE69034037T2/de not_active Expired - Fee Related
- 1990-09-10 CA CA002066576A patent/CA2066576A1/en not_active Abandoned
1992
- 1992-03-16 US US07/838,729 patent/US5864021A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
EP0722951B1 (de)	2003-01-15
EP1132469A2 (de)	2001-09-12
JPH05500898A (ja)	1993-02-25
DE69027995T2 (de)	1997-02-27
DK0491849T3 (da)	1996-12-30
ES2093031T3 (es)	1996-12-16
DE69027995D1 (de)	1996-09-05
DE69034037D1 (de)	2003-02-20
EP1104807A2 (de)	2001-06-06
ATE231165T1 (de)	2003-02-15
US5864021A (en)	1999-01-26
AU6504490A (en)	1991-04-18
EP0722951A2 (de)	1996-07-24
EP1132469A3 (de)	2001-11-28
DK0722951T3 (da)	2003-03-24
EP0491849B1 (de)	1996-07-31
WO1991004267A1 (en)	1991-04-04
ES2188632T3 (es)	2003-07-01
US5043431A (en)	1991-08-27
CA2066576A1 (en)	1991-03-12
DE69034037T2 (de)	2003-08-28
EP0722951A3 (de)	1996-10-23
EP0491849A1 (de)	1992-07-01
EP0491849A4 (en)	1993-04-07

Legal Events

Date	Code	Title	Description
1997-03-15	UEP	Publication of translation of european patent specification
2004-06-15	REN	Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
DE69027995D1 (de)	1996-09-05	Reinigungsverfahren von transformierendem wachstumsfaktor (tgf)
MY103892A (en)	1993-10-30	Interleukin-2 of high purity production and use
EP0391526A3 (de)	1991-01-09	Reinigungsverfahren für Antikörper
AU3143589A (en)	1989-09-21	Human nerve growth factor
FR2564859B1 (fr)	1987-09-25	Procede de preparation d'acide hyaluronique par fermentation.
CA2220515A1 (en)	1996-11-14	Process for producing erythropoietin containing no animal proteins
TNSN88111A1 (fr)	1990-07-10	Procede de purification d'une proteine
KR900009989A (ko)	1990-07-06	내피세포 생장 인자
EP0233620A3 (en)	1988-02-03	Adsorbent for purification of blood coagulation factor viii and process for purification of blood coagulation factor viii using the same
ATE136552T1 (de)	1996-04-15	Reinigung von menschlichem, aus einer escherichia coli mutante abgesonderten interleukin 4
Lucki-Lange et al.	1991	Conditions for the production of recombinant IL-2 in stirred suspension culture using a protein free medium
AU1239795A (en)	1995-07-13	Purified stable liquid prealbumin
AU7955687A (en)	1989-04-13	Method for purification of antibodies
KR920012437A (ko)	1992-07-27	인간 인슐린 유사 성장인자-i의 정제 방법
FR2611743B1 (fr)	1994-06-10	Procede pour la production de l-aminoacides ou d'acide 5'-inosinique par fermentation
DE227102T1 (de)	1987-11-26	Errichtete zellinie aus menschlichem normalem gebaermuttermuskel, verfahren zur herstellung des plasminogenaktivators mittels dieser zellinie und plasminogenaktivator.
JPS5618591A (en)	1981-02-21	Production of substance capable of differentiation and multiplication of stem cells of human granulocyte
JPS6463600A (en)	1989-03-09	Method for purifying vitronectin
KR920012436A (ko)	1992-07-27	인간 인슐린 유사 성장인자-i의 정제 방법
JPS5682095A (en)	1981-07-04	Purification of fatty acid produced through fermentation process
RU93051501A (ru)	1996-04-20	Очистка человеческого рекомбинантного эритропоэтина
EP0309111A3 (de)	1990-04-18	Verfahren zur Erzielung höherer Ausbeuten an nichtaggregiertem Gewebeplasminogenaktivator aus Zellkulturen
JPS61181395A (ja)	1986-08-14	マウスインタ−フエロンの精製法
MY102245A (en)	1992-05-15	Purification of antibodies