ATE231165T1 - Verfahren zur herstellung von tgf-beta - Google Patents

Verfahren zur herstellung von tgf-beta

Info

Publication number: ATE231165T1
Authority: AT; Austria
Prior art keywords: tgf; sup; cation exchange; reactor; beta
Prior art date: 1989-09-11

Application number

AT95119910T

Other languages

English (en)

Inventor

Erno Pungaor Jr

Eirik Nestaas

Original Assignee

Berlex Lab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-09-11

Filing date

1990-09-10

Publication date

2003-02-15

1990-09-10 Application filed by Berlex Lab filed Critical Berlex Lab

2003-02-15 Application granted granted Critical

2003-02-15 Publication of ATE231165T1 publication Critical patent/ATE231165T1/de

Links

ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 title abstract 5
238000004519 manufacturing process Methods 0.000 title 1
102000004887 Transforming Growth Factor beta Human genes 0.000 abstract 4
108090001012 Transforming Growth Factor beta Proteins 0.000 abstract 4
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 abstract 1
102000046299 Transforming Growth Factor beta1 Human genes 0.000 abstract 1
101800002279 Transforming growth factor beta-1 Proteins 0.000 abstract 1
238000005277 cation exchange chromatography Methods 0.000 abstract 1
239000003729 cation exchange resin Substances 0.000 abstract 1
239000003636 conditioned culture medium Substances 0.000 abstract 1
230000000694 effects Effects 0.000 abstract 1
230000004151 fermentation Effects 0.000 abstract 1
238000000855 fermentation Methods 0.000 abstract 1
238000004191 hydrophobic interaction chromatography Methods 0.000 abstract 1
239000000203 mixture Substances 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
230000010412 perfusion Effects 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1
108090000623 proteins and genes Proteins 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/495—Transforming growth factor [TGF]

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Genetics & Genomics (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Medicinal Chemistry (AREA)
Toxicology (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biophysics (AREA)
Microbiology (AREA)
Sustainable Development (AREA)
Animal Behavior & Ethology (AREA)
Veterinary Medicine (AREA)
Pharmacology & Pharmacy (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Public Health (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Steroid Compounds (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)

AT95119910T 1989-09-11 1990-09-10 Verfahren zur herstellung von tgf-beta ATE231165T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US07/405,695 US5043431A (en)	1989-09-11	1989-09-11	Method and apparatus for the production of TGF-β

Publications (1)

Publication Number	Publication Date
ATE231165T1 true ATE231165T1 (de)	2003-02-15

Family

ID=23604819

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
AT90914805T ATE140927T1 (de)	1989-09-11	1990-09-10	Reinigungsverfahren von transformierendem wachstumsfaktor (tgf)
AT95119910T ATE231165T1 (de)	1989-09-11	1990-09-10	Verfahren zur herstellung von tgf-beta

Family Applications Before (1)

Application Number	Title	Priority Date	Filing Date
AT90914805T ATE140927T1 (de)	1989-09-11	1990-09-10	Reinigungsverfahren von transformierendem wachstumsfaktor (tgf)

Country Status (10)

Country	Link
US (2)	US5043431A (de)
EP (4)	EP1132469A3 (de)
JP (1)	JPH05500898A (de)
AT (2)	ATE140927T1 (de)
AU (1)	AU6504490A (de)
CA (1)	CA2066576A1 (de)
DE (2)	DE69027995T2 (de)
DK (2)	DK0491849T3 (de)
ES (2)	ES2188632T3 (de)
WO (1)	WO1991004267A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5221734A (en) *	1987-10-01	1993-06-22	Ciba-Geigy Corporation	Process for preparing a polypeptide growth factor for milk
US5043431A (en) *	1989-09-11	1991-08-27	Codon	Method and apparatus for the production of TGF-β
US5206023A (en) *	1991-01-31	1993-04-27	Robert F. Shaw	Method and compositions for the treatment and repair of defects or lesions in cartilage
US5270300A (en) *	1991-09-06	1993-12-14	Robert Francis Shaw	Methods and compositions for the treatment and repair of defects or lesions in cartilage or bone
JP3386498B2 (ja) *	1992-11-06	2003-03-17	森永製菓株式会社	ヒトＴＧＦ−βの精製法
US5331095A (en) *	1993-04-12	1994-07-19	Scios Nova Inc.	Process for purification of basic fibroblast growth factor
WO2000034521A1 (en) *	1998-12-08	2000-06-15	Boston Probes, Inc.	Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
DE19918409A1 (de) *	1999-04-22	2000-10-26	Forschungszentrum Juelich Gmbh	Bioreaktor umfassend einen Medienablauf und einen Rührer sowie Rührer und Medienablauf
FR2827290B1 (fr) *	2001-07-13	2004-07-09	Pierre Jouan Biotechnologies Sa	Procede d'obtention d'une fraction proteique enrichie en tgf-beta sous forme activee, fraction proteique et applications therapeutiques
US6849394B2 (en) *	2002-02-21	2005-02-01	Minitube Of America	Compositions comprising reproductive cell media and methods for using such compositions
EP1728852A1 (de) *	2005-05-30	2006-12-06	Chiron Behring GmbH & Co. KG	Fermentersystem für Biotechnischen Prozessen
US20110207165A1 (en) *	2008-10-06	2011-08-25	Hoffman-La Roche, Inc.	Small scale shaker flask cultivation
KR20170138436A (ko)	2015-03-17	2017-12-15	이스티튜토 바이오키미코 이탈리아노 지오바니 로렌치니 에스.피.에이.	골 형성 단백질 (bpm)의 정제
GB201509193D0 (en) *	2015-05-28	2015-07-15	Ge Healthcare Bio Sciences Ab	Method and system for cell cultivation
US9617191B1 (en) *	2016-06-09	2017-04-11	Xianggen Wu	Bioreactor system and method

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5630925A (en) *	1979-08-21	1981-03-28	Sumitomo Chem Co Ltd	Purification of human growth hormone
US4296205A (en) *	1980-02-22	1981-10-20	Verma Dharmvir S	Cell culture and continuous dialysis flask and method
US4427580A (en) *	1982-09-01	1984-01-24	Cornell Research Foundation, Inc.	Method for separation and recovery of proteins and nucleic acids from nucleoproteins using water destructuring salts
US4444760A (en) *	1983-06-17	1984-04-24	Merck & Co., Inc.	Purification and characterization of a protein fibroblast growth factor
US4897464A (en) *	1985-04-17	1990-01-30	President And Fellows Of Harvard College	Purified protein having angiogenic activity and methods of preparation
US4751078A (en) *	1985-07-03	1988-06-14	Nagabhushan Tattanahalli L	Process for the purification of gamma interferon
US4894439A (en) *	1986-05-22	1990-01-16	Cetus Corporation	N-terminal derivatives of tumor necrosis factor purified by microporous PTFE membranes
PT86144B (pt) *	1986-11-17	1990-08-31	Sandoz Sa	Processo para a preparacao de uma proteina tendo actividade semelhante a factor g-tsf humano e de composicoes farmaceuticas que o contem
US4931548A (en) *	1987-01-30	1990-06-05	Techne Corporation	Heterodimer form of transforming growth factor-beta
JPH01157384A (ja) *	1987-09-03	1989-06-20	Invitron Corp	非会合の組織プラスミノーゲン活性化因子を培養細胞から高収率で得る方法
EP0323842A3 (de) *	1988-01-07	1990-04-25	Otsuka Pharmaceutical Co., Ltd.	Methode für die Säuberung von umbildendem Wachstums-Faktor-Beta
EP0338716A3 (de) *	1988-04-21	1990-04-11	Berlex Laboratories, Inc.	Methode und Vorrichtung zur Produktion nichtdegradierter Proteine aus Säugetierzellen
US5043431A (en) *	1989-09-11	1991-08-27	Codon	Method and apparatus for the production of TGF-β

1989
- 1989-09-11 US US07/405,695 patent/US5043431A/en not_active Expired - Fee Related
1990
- 1990-09-10 AT AT90914805T patent/ATE140927T1/de not_active IP Right Cessation
- 1990-09-10 AT AT95119910T patent/ATE231165T1/de not_active IP Right Cessation
- 1990-09-10 EP EP00250419A patent/EP1132469A3/de not_active Withdrawn
- 1990-09-10 CA CA002066576A patent/CA2066576A1/en not_active Abandoned
- 1990-09-10 DE DE69027995T patent/DE69027995T2/de not_active Expired - Lifetime
- 1990-09-10 DK DK90914805.8T patent/DK0491849T3/da active
- 1990-09-10 JP JP2513740A patent/JPH05500898A/ja active Pending
- 1990-09-10 EP EP00250420A patent/EP1104807A2/de not_active Withdrawn
- 1990-09-10 ES ES95119910T patent/ES2188632T3/es not_active Expired - Lifetime
- 1990-09-10 EP EP90914805A patent/EP0491849B1/de not_active Expired - Lifetime
- 1990-09-10 DE DE69034037T patent/DE69034037T2/de not_active Expired - Fee Related
- 1990-09-10 EP EP95119910A patent/EP0722951B1/de not_active Expired - Lifetime
- 1990-09-10 ES ES90914805T patent/ES2093031T3/es not_active Expired - Lifetime
- 1990-09-10 DK DK95119910T patent/DK0722951T3/da active
- 1990-09-10 AU AU65044/90A patent/AU6504490A/en not_active Abandoned
- 1990-09-10 WO PCT/US1990/005098 patent/WO1991004267A1/en not_active Ceased
1992
- 1992-03-16 US US07/838,729 patent/US5864021A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
DK0491849T3 (da)	1996-12-30
EP0491849B1 (de)	1996-07-31
EP0722951A2 (de)	1996-07-24
DE69027995T2 (de)	1997-02-27
ES2188632T3 (es)	2003-07-01
CA2066576A1 (en)	1991-03-12
EP1132469A2 (de)	2001-09-12
EP0491849A1 (de)	1992-07-01
DE69034037D1 (de)	2003-02-20
DE69027995D1 (de)	1996-09-05
US5043431A (en)	1991-08-27
EP0491849A4 (en)	1993-04-07
ATE140927T1 (de)	1996-08-15
DE69034037T2 (de)	2003-08-28
EP0722951A3 (de)	1996-10-23
ES2093031T3 (es)	1996-12-16
AU6504490A (en)	1991-04-18
DK0722951T3 (da)	2003-03-24
WO1991004267A1 (en)	1991-04-04
JPH05500898A (ja)	1993-02-25
EP0722951B1 (de)	2003-01-15
EP1104807A2 (de)	2001-06-06
EP1132469A3 (de)	2001-11-28
US5864021A (en)	1999-01-26

Legal Events

Date

Code

Title

Description

2003-07-15

UEP

Publication of translation of european patent specification

Ref document number: 0722951

Country of ref document: EP

2004-12-15

REN

Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
ATE231165T1 (de)	2003-02-15	Verfahren zur herstellung von tgf-beta
ES8606489A1 (es)	1986-04-01	Un metodo de producir una proteina de interleuquina-2-humana no glicosilada sustancialmente pura
DE69334327D1 (de)	2010-05-27	Verfahren zur Herstellung sekretierter Proteine
DE69001132D1 (de)	1993-04-29	Verfahren zur herstellung von mikrokapseln.
DE68925083D1 (de)	1996-01-25	Rekombinante DNS, diese rekombinante DNS enthaltender Mikroorganismus und Verfahren zur Herstellung von L-Aminosäure unter Verwendung dieses Mikroorganismus
EP0391526A3 (de)	1991-01-09	Reinigungsverfahren für Antikörper
ZA891962B (en)	1989-11-29	Human nerve growth factor
US4514387A (en)	1985-04-30	Chemokinesins and chemotaxins of leukocytes and inflamed tissues
TNSN88111A1 (fr)	1990-07-10	Procede de purification d'une proteine
ES2059854T3 (es)	1994-11-16	Nuevo polipeptido y su produccion.
US4404280A (en)	1983-09-13	Process for preparing murine interleukin 2 in the presence of interleukin 1 from an interleukin 2 nonproducer malignant cell line and cell line therefor
CA2063511A1 (en)	1992-09-21	Method for producing human serum albumin
DE69430975D1 (de)	2002-08-22	Verfahren zur Herstellung von Proteinen
US4189535A (en)	1980-02-19	Serum cell growth promoting materials
DE69017793D1 (de)	1995-04-20	Produktion von menschlichen Nervenwachstumsfaktoren.
JP2500384B2 (ja)	1996-05-29	生理活性物質の製造法
JPS63192381A (ja)	1988-08-09	無血清培地で増殖継代可能な細胞およびその取得方法
ATE47427T1 (de)	1989-11-15	Verfahren zur herstellung von arphamenin mit hoher ausbeute.
DE227102T1 (de)	1987-11-26	Errichtete zellinie aus menschlichem normalem gebaermuttermuskel, verfahren zur herstellung des plasminogenaktivators mittels dieser zellinie und plasminogenaktivator.
JPS57181695A (en)	1982-11-09	Production of heterophile antibody
JPS63185375A (ja)	1988-07-30	動物細胞増殖用組成物および増殖方法
RU93051501A (ru)	1996-04-20	Очистка человеческого рекомбинантного эритропоэтина
JPH04320681A (ja)	1992-11-11	動物細胞の培養方法