ATE140973T1 - Ausscheidung von heterologen proteinen von e. coli - Google Patents

Ausscheidung von heterologen proteinen von e. coli

Info

Publication number
ATE140973T1
ATE140973T1 AT89308753T AT89308753T ATE140973T1 AT E140973 T1 ATE140973 T1 AT E140973T1 AT 89308753 T AT89308753 T AT 89308753T AT 89308753 T AT89308753 T AT 89308753T AT E140973 T1 ATE140973 T1 AT E140973T1
Authority
AT
Austria
Prior art keywords
coli
coding
excretion
heterologue
proteins
Prior art date
Application number
AT89308753T
Other languages
English (en)
Inventor
Raymond W K Wong
Margaret L Sutherland
Original Assignee
Allelix Biopharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Allelix Biopharma filed Critical Allelix Biopharma
Application granted granted Critical
Publication of ATE140973T1 publication Critical patent/ATE140973T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5412—IL-6
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70514—CD4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT89308753T 1988-08-31 1989-08-30 Ausscheidung von heterologen proteinen von e. coli ATE140973T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23914588A 1988-08-31 1988-08-31
US07/395,797 US5223407A (en) 1988-08-31 1989-08-18 Excretion of heterologous proteins from e. coli

Publications (1)

Publication Number Publication Date
ATE140973T1 true ATE140973T1 (de) 1996-08-15

Family

ID=26932319

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89308753T ATE140973T1 (de) 1988-08-31 1989-08-30 Ausscheidung von heterologen proteinen von e. coli

Country Status (7)

Country Link
US (1) US5223407A (de)
EP (1) EP0357391B1 (de)
JP (1) JP2930984B2 (de)
AT (1) ATE140973T1 (de)
DE (1) DE68926895T2 (de)
ES (1) ES2091197T3 (de)
HK (1) HK1008048A1 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5646015A (en) * 1988-08-31 1997-07-08 Astra Ab Excretion of heterologous proteins from E. coli
USRE37919E1 (en) 1989-05-12 2002-12-03 The General Hospital Corporation Recombinant DNA method for production of parathyroid hormone
WO1991005863A1 (en) * 1989-10-11 1991-05-02 Pitman-Moore Australia Limited Recombinant growth factors
JPH05219963A (ja) * 1990-07-18 1993-08-31 Applied Immune Sci Inc 温度誘導分泌発現ベクターおよびその原核細胞での使用
DE69130872T2 (de) * 1990-11-08 1999-08-26 Japan Energy Corp. Sekretionsvektor, diesen enthaltene transformierte Mikroorganismen und Herstellung von Produkten durch obigen Mikroorganismus
US5208041A (en) * 1991-05-23 1993-05-04 Allelix Biopharmaceuticals Inc. Essentially pure human parathyroid hormone
US5382658A (en) * 1992-04-03 1995-01-17 Allelix Biopharmaceuticals Inc. Stability-enhanced variants of parathyroid hormone
US5290920A (en) * 1992-04-16 1994-03-01 Allelix Biopharmaceuticals Inc. Method of purifying human epidermal growth factor
CA2098639A1 (en) * 1992-06-19 1993-12-20 K. Anne Kronis Bone stimulating, non-vasoactive parathyroid hormone variants
SE9203753D0 (sv) * 1992-12-11 1992-12-11 Kabi Pharmacia Ab Expression system for producing apolipoprotein ai-m
AU6582594A (en) * 1993-04-26 1994-11-21 Dae Woong Pharmaceutical Co., Ltd. A novel gene coding human epidermal growth factor and process for preparing the same
US5629205A (en) * 1995-05-19 1997-05-13 Allelix Biopharmaceuticals Inc. Promoters for gene expression
AU742270B2 (en) 1997-04-16 2001-12-20 Enteris Biopharma, Inc. Direct expression of peptides into culture media
SE9702401D0 (sv) * 1997-06-19 1997-06-19 Astra Ab Pharmaceutical use
US6753165B1 (en) * 1999-01-14 2004-06-22 Bolder Biotechnology, Inc. Methods for making proteins containing free cysteine residues
US7270809B2 (en) * 1997-07-14 2007-09-18 Bolder Biotechnology, Inc. Cysteine variants of alpha interferon-2
US7153943B2 (en) * 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
ATE375363T1 (de) * 1997-07-14 2007-10-15 Bolder Biotechnology Inc Derivate des wachstumshormons und verwandte proteine
US20080076706A1 (en) 1997-07-14 2008-03-27 Bolder Biotechnology, Inc. Derivatives of Growth Hormone and Related Proteins, and Methods of Use Thereof
US6146848A (en) * 1998-07-23 2000-11-14 The Hong Kong University Of Science & Technology Bacterial expression system
US8288126B2 (en) 1999-01-14 2012-10-16 Bolder Biotechnology, Inc. Methods for making proteins containing free cysteine residues
NZ522847A (en) 2000-05-16 2004-11-26 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
US6875590B2 (en) * 2000-06-05 2005-04-05 Corixa Corporation Leader peptides for enhancing secretion of recombinant protein from a host cell
GB0027782D0 (en) * 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods fro large scale protein productio in prokaryotes
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
US6955910B2 (en) 2000-11-14 2005-10-18 Boehringer Ingelheim International Gmbh Method for large scale production of recombinant DNA-derived TPA or K2S molecules
WO2005046798A1 (en) * 2003-11-12 2005-05-26 Nps Allelix Corp. Treatment of bone loss utilizing full length parathyroid hormone
US8227241B2 (en) * 2004-03-12 2012-07-24 Unigene Laboratories, Inc. Bacterial host cell for the direct expression of peptides
PT1802334E (pt) 2004-10-21 2012-11-28 Genentech Inc Método para tratamento de doenças neovasculares intraoculares
GB0602173D0 (en) * 2006-02-03 2006-03-15 Avecia Ltd Expression system
JP5130662B2 (ja) * 2006-06-07 2013-01-30 Ｊｎｃ株式会社 タンパク質の可溶性タンパク質としての製造方法
US8617531B2 (en) * 2006-12-14 2013-12-31 Bolder Biotechnology, Inc. Methods of making proteins and peptides containing a single free cysteine
KR100844992B1 (ko) 2007-01-02 2008-07-08 한국과학기술원 ＯｍｐＦ와 인체 유래 상피세포성장인자의 동시 발현을통한 인체 유래 상피세포성장인자의 세포외 분비·생산 방법
US9017968B2 (en) 2012-11-09 2015-04-28 Gene-Vinate Limited Means and methods for producing authentic human basic fibroblast growth factor
WO2024124138A2 (en) * 2022-12-08 2024-06-13 The Board Of Trustees Of The University Of Illinois A molecular toolkit for heterologous protein secretion in bacteroides species

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4595658A (en) * 1982-09-13 1986-06-17 The Rockefeller University Method for facilitating externalization of proteins synthesized in bacteria
US4637980A (en) * 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
EP0177343B1 (de) * 1984-10-05 1992-07-22 Genentech, Inc. DNA, Zellkulturen und Verfahren zur Sekretion von heterologen Proteinen und periplasmische Proteinrückgewinnung
HU204563B (en) * 1984-12-06 1992-01-28 Synergen Biolog Inc Process for producing recombinant serine-protease inhibitors and dns sequencyes for them
US4994559A (en) * 1985-12-17 1991-02-19 Synergen, Inc. Human basic fibroblast growth factor
IE67035B1 (en) * 1986-07-08 1996-02-21 Genetics Inst Production and use of non-glycosylated IL-6
AU8172787A (en) * 1986-10-30 1988-05-25 Synergen Biologicals, Inc. Human pancreatic secretory trypsin inhibitors produced by recombinant dna methods and processes for the production of same

Also Published As

Publication number Publication date
ES2091197T3 (es) 1996-11-01
JPH02177889A (ja) 1990-07-10
JP2930984B2 (ja) 1999-08-09
EP0357391A3 (de) 1991-07-31
EP0357391B1 (de) 1996-07-31
US5223407A (en) 1993-06-29
HK1008048A1 (en) 1999-04-30
EP0357391A2 (de) 1990-03-07
DE68926895T2 (de) 1997-03-13
DE68926895D1 (de) 1996-09-05

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Legal Events

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EELA Cancelled due to lapse of time