ATE142694T1 - Neues verfahren zur produktion von nichtfusioniertem protein in e. coli - Google Patents

Neues verfahren zur produktion von nichtfusioniertem protein in e. coli

Info

Publication number
ATE142694T1
ATE142694T1 AT90810890T AT90810890T ATE142694T1 AT E142694 T1 ATE142694 T1 AT E142694T1 AT 90810890 T AT90810890 T AT 90810890T AT 90810890 T AT90810890 T AT 90810890T AT E142694 T1 ATE142694 T1 AT E142694T1
Authority
AT
Austria
Prior art keywords
rigf
fusioned
coli
protein
new method
Prior art date
Application number
AT90810890T
Other languages
English (en)
Inventor
Albert Dr Schmitz
Walter Dr Maerki
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Application granted granted Critical
Publication of ATE142694T1 publication Critical patent/ATE142694T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT90810890T 1989-11-29 1990-11-20 Neues verfahren zur produktion von nichtfusioniertem protein in e. coli ATE142694T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898927008A GB8927008D0 (en) 1989-11-29 1989-11-29 Novel process for the production of unfused protein in e.coli

Publications (1)

Publication Number Publication Date
ATE142694T1 true ATE142694T1 (de) 1996-09-15

Family

ID=10667136

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90810890T ATE142694T1 (de) 1989-11-29 1990-11-20 Neues verfahren zur produktion von nichtfusioniertem protein in e. coli

Country Status (12)

Country Link
US (2) US5210028A (de)
EP (1) EP0434605B1 (de)
JP (2) JPH03228682A (de)
AT (1) ATE142694T1 (de)
CA (1) CA2030947A1 (de)
DE (1) DE69028499T2 (de)
DK (1) DK0434605T3 (de)
ES (1) ES2091237T3 (de)
GB (1) GB8927008D0 (de)
GR (1) GR3020993T3 (de)
IE (1) IE74173B1 (de)
PT (1) PT96004B (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ207842A (en) * 1983-04-25 1988-02-12 Chiron Corp Production of human insulin-like growth factor (igf) using cdna
IL71991A (en) * 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
US5532142A (en) * 1993-02-12 1996-07-02 Board Of Regents, The University Of Texas System Method of isolation and purification of fusion polypeptides
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
KR100309700B1 (ko) * 1994-01-15 2001-12-15 성재갑 재조합단백질의활성상태로의재구성방법
EP0779927A1 (de) * 1994-09-08 1997-06-25 Chiron Corporation Methode zur gesteigerten herstellung von insulin-ähnlichem wachstumsfaktor
EP1095056B1 (de) * 1998-07-09 2006-12-27 BaroFold, Inc. Rückfaltung von protein-aggregaten und einschlussteilchen durch hohen druck
US6121421A (en) * 1998-08-21 2000-09-19 Abbott Laboratories Methods for isolating recombinant β-casein
CA2434045A1 (en) * 2000-10-31 2002-08-15 The Regents Of The University Of Colorado, A Body Corporate Improved protein disaggregation and refolding using high pressure
US7105327B1 (en) * 2001-06-15 2006-09-12 Bharat Biotech International, Ltd. Recombinant streptokinase
WO2003066676A1 (en) * 2002-02-05 2003-08-14 Geymonat S.P.A. Production process of recombinant placental growth factor
WO2003085108A1 (fr) * 2002-04-10 2003-10-16 Ajinomoto Co., Inc. Adn recombinant presentant un gene d'hydantoinase et un gene de carbamylase et procede de production d'acide amine
US6969769B2 (en) * 2002-06-14 2005-11-29 Vanson Halosource, Inc. N-halamine siloxanes for use in biocidal coatings and materials
CA2663416A1 (en) * 2006-09-15 2008-03-20 Barofold, Inc. High pressure treatment of proteins for reduced immunogenicity
WO2011057021A1 (en) * 2009-11-05 2011-05-12 Quest Diagnostics Investments Incorporated Quantitation of insulin-like growth factor-i and insulin-like growth factor-ii with high-resolution mass spectrometry
CN104911203A (zh) * 2015-06-30 2015-09-16 成都易胜科生物科技有限公司 一种重组人胰岛素的工业制备方法

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2586170A (en) * 1946-10-04 1952-02-19 Peerless Tool & Engineering Co Fishing tackle assembly
US2807905A (en) * 1955-11-07 1957-10-01 Jesse E Ford Fishing lure and plug retriever
US2809460A (en) * 1956-03-23 1957-10-15 Belty L Taylor Fishing lure retriever
US2807906A (en) * 1956-08-06 1957-10-01 Mun Henry Chan Fishing tackle retriever
US3144728A (en) * 1962-02-19 1964-08-18 Stevens Marvin Lure retriever
US3156064A (en) * 1963-08-21 1964-11-10 Herbert J Czirr Means to recover a lure or the like
US4408411A (en) * 1981-05-21 1983-10-11 Skarnells David B Fishing lure retriever
US4620948A (en) * 1982-12-22 1986-11-04 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4536984A (en) * 1983-02-18 1985-08-27 Leon Kowal Retrieval device for fishing tackle
NZ207842A (en) * 1983-04-25 1988-02-12 Chiron Corp Production of human insulin-like growth factor (igf) using cdna
WO1985000831A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of insulin-like growth factor
US4758512A (en) * 1984-03-06 1988-07-19 President And Fellows Of Harvard College Hosts and methods for producing recombinant products in high yields
DE3581528D1 (de) * 1984-07-13 1991-02-28 Chiron Corp Insulinaehnlicher wachstumsfaktor ii.
US4738921A (en) * 1984-09-27 1988-04-19 Eli Lilly And Company Derivative of the tryptophan operon for expression of fused gene products
EP0193112A3 (de) * 1985-02-22 1987-02-25 The Trustees Of Columbia University In The City Of New York Für einen insulinähnlichen Säugetier-Wachstumsfaktor II kodierende cDNS
SE8505922D0 (sv) * 1985-12-13 1985-12-13 Kabigen Ab Construction of an igg binding protein to facilitate downstream processing using protein engineering
US4885163A (en) * 1987-02-24 1989-12-05 Eli Lilly And Company Topical use of IGF-II for wound healing
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
EP0289314B1 (de) * 1987-04-28 1994-10-12 Roche Diagnostics GmbH Verwendung von IGF-II zur Behandlung von Knochenkrankheiten
US4961969A (en) * 1987-05-11 1990-10-09 Cetus Corporation Process for recovering microbially produced interferon-β
GB8723660D0 (en) * 1987-10-08 1987-11-11 British Bio Technology Synthetic gene

Also Published As

Publication number Publication date
JPH03228682A (ja) 1991-10-09
GB8927008D0 (en) 1990-01-17
EP0434605A2 (de) 1991-06-26
DE69028499D1 (de) 1996-10-17
IE74173B1 (en) 1997-07-02
DK0434605T3 (da) 1996-09-30
GR3020993T3 (en) 1996-12-31
IE904294A1 (en) 1991-06-05
JP2001008697A (ja) 2001-01-16
PT96004A (pt) 1991-09-13
DE69028499T2 (de) 1997-03-13
CA2030947A1 (en) 1991-05-30
PT96004B (pt) 1998-01-30
EP0434605A3 (en) 1991-11-06
EP0434605B1 (de) 1996-09-11
US5708148A (en) 1998-01-13
ES2091237T3 (es) 1996-11-01
US5210028A (en) 1993-05-11

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