ATE151433T1 - Verfahren und kit zur reinigung von nukleinsäure - Google Patents

Verfahren und kit zur reinigung von nukleinsäure

Info

Publication number
ATE151433T1
ATE151433T1 AT90911986T AT90911986T ATE151433T1 AT E151433 T1 ATE151433 T1 AT E151433T1 AT 90911986 T AT90911986 T AT 90911986T AT 90911986 T AT90911986 T AT 90911986T AT E151433 T1 ATE151433 T1 AT E151433T1
Authority
AT
Austria
Prior art keywords
kit
nucleic acid
purifying nucleic
surfactant
amphotenic
Prior art date
Application number
AT90911986T
Other languages
English (en)
Inventor
Melissa Reardon
Lisa Klein
Original Assignee
Boehringer Mannheim Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Corp filed Critical Boehringer Mannheim Corp
Application granted granted Critical
Publication of ATE151433T1 publication Critical patent/ATE151433T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT90911986T 1989-11-13 1990-07-31 Verfahren und kit zur reinigung von nukleinsäure ATE151433T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/434,324 US4997932A (en) 1989-11-13 1989-11-13 Method and kit for purifying nucleic acids

Publications (1)

Publication Number Publication Date
ATE151433T1 true ATE151433T1 (de) 1997-04-15

Family

ID=23723768

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90911986T ATE151433T1 (de) 1989-11-13 1990-07-31 Verfahren und kit zur reinigung von nukleinsäure

Country Status (6)

Country Link
US (1) US4997932A (de)
EP (3) EP0747387A3 (de)
AT (1) ATE151433T1 (de)
DE (2) DE69033304T2 (de)
ES (1) ES2100887T3 (de)
WO (1) WO1991007422A1 (de)

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US5700636A (en) * 1990-10-19 1997-12-23 Becton Dickinson And Company Methods for selectively detecting microorganisms associated with vaginal infections in complex biological samples
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
US5284940A (en) * 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
SE503729C2 (sv) * 1991-03-11 1996-08-12 Mats Malmquist Extraktionssystem
DE4143639C2 (de) * 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
IT1252174B (it) * 1991-12-09 1995-06-05 Crinos Industria Farmaco Oligodesossimibonucleotidi ad attivita' antiischemica e procedimenti per il loro ottenimento
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
WO1994000474A1 (en) * 1992-06-25 1994-01-06 Life Technologies, Inc. Method for the rapid and ultra-sensitive detection and quantification of nucleic acid
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
IL110463A0 (en) * 1993-08-13 1994-10-21 Du Pont In situ extraction of microbial DNA
US5990301A (en) * 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
DK0775150T3 (da) * 1994-02-07 1999-11-08 Qiagen Gmbh Fremgangsmåde til reduktion eller fjernelse af endotoksiner
US5545539A (en) * 1994-10-18 1996-08-13 Genzyme Corporation Method for nucleotide sequence amplification
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
CA2212446C (en) * 1995-12-21 2010-09-21 Genzyme Corporation Method for lysing cells
ES2188926T3 (es) 1996-03-20 2003-07-01 Bio Merieux Aislamiento del acido nucleico.
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
CA2294749C (en) * 1997-06-27 2007-01-02 Life Technologies, Inc. One step device and process for concentration and purification of biological molecules
US5958677A (en) * 1997-07-28 1999-09-28 The New York Blood Center, Inc. Method for purifying viral nucleic acids
JP2001516731A (ja) * 1997-09-17 2001-10-02 ジエントラ・システムズ・インコーポレーテツド 核酸を単離するための装置および方法
WO1999022009A1 (en) 1997-10-24 1999-05-06 Megabios Corporation Methods for preparing polynucleotide transfection complexes
US6241980B1 (en) 1997-11-04 2001-06-05 Becton, Dickinson And Company Sample processing method using ion exchange resin
ZA99493B (en) * 1998-01-30 1999-07-22 Akzo Nobel Nv Method for the isolation of nucleic acid.
US6750059B1 (en) 1998-07-16 2004-06-15 Whatman, Inc. Archiving of vectors
US6441152B1 (en) * 1998-12-08 2002-08-27 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
DE19903507A1 (de) 1999-01-29 2000-08-10 Roche Diagnostics Gmbh Verfahren zur Herstellung endotoxinfreier oder an Endotoxin abgereicherter Nukleinsäuren und deren Verwendung
AU4367500A (en) * 1999-04-21 2000-11-02 Annovis, Inc. Magnetic dna extraction kit for plants
EP1301591A4 (de) * 2000-07-13 2004-05-26 Invitrogen Corp Verfahren und zusammensetzungen zur schnellen protein- und peptidextraktion und -isolierung mittels lysismatrize
EP1305328A2 (de) * 2000-07-21 2003-05-02 Mark B. Lyles Materialien und verfahren zum anlagern von nukleinsäure auf oberflächen
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6833238B2 (en) * 2002-01-04 2004-12-21 Applera Corporation Petal-array support for use with microplates
CA2473376A1 (en) 2002-01-16 2003-07-31 Invitrogen Dynal As Method for isolating nucleic acids and protein from a single sample
WO2003064605A2 (en) 2002-01-28 2003-08-07 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
DE10231659B4 (de) * 2002-07-12 2006-01-19 Preanalytix Gmbh Zusammensetzung zum Binden von Nukleinsäure an eine Festphase
US20040016702A1 (en) * 2002-07-26 2004-01-29 Applera Corporation Device and method for purification of nucleic acids
JP2005538717A (ja) * 2002-09-13 2005-12-22 ヴァレンティス，インコーポレイテッド 核酸のプレパラティブスケールの精製のための装置および方法
WO2005012487A2 (en) * 2003-08-01 2005-02-10 Invitrogen Corporation Compositions and methods for preparing short rna molecules and other nucleic acids
US20050106577A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Cleavable solid phases for isolating nucleic acids
US20050136477A1 (en) * 2003-11-17 2005-06-23 Hashem Akhavan-Tafti Methods for isolating nucleic acids from biological and cellular materials
US20050106602A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Simplified methods for isolating nucleic acids from cellular materials
US20050106576A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Methods of using cleavable solid phases for isolating nucleic acids
WO2005061532A1 (es) * 2003-12-23 2005-07-07 Berthet Francois Xavier Composiciones y procedimientos para detectar infección patógena
WO2005068662A1 (en) * 2003-12-30 2005-07-28 Sigma-Aldrich Co. Rapid preparation of nucleic acids by enzymatic digestion
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
DE102005057334A1 (de) * 2005-11-28 2007-06-06 Aj Innuscreen Gmbh Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien
WO2007149350A2 (en) * 2006-06-19 2007-12-27 Becton, Dickinson And Company Methods and compositions for obtaining amplifiable nucleic acids from tissues, cells, or viruses exposed to transport media
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
EP2247753B1 (de) * 2008-02-01 2014-06-25 Siemens Healthcare Diagnostics Inc. Urintransportmittel
WO2010071833A1 (en) 2008-12-19 2010-06-24 Life Technologies Corporation Proteinase k inhibitors, methods and compositions therefor
BR112012004699B1 (pt) 2009-09-03 2019-10-15 Becton, Dickinson And Company Métodos, composições e kits para lise química direta
US9212976B2 (en) * 2013-03-15 2015-12-15 Qiagen Gaithersburg, Inc. Vision-guided aspiration apparatus and methods
EP2970962A4 (de) * 2013-03-15 2017-03-08 Pathogenetix, Inc. Verfahren und vorrichtungen zur herstellung biologischer proben

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FR2471785A1 (fr) * 1979-12-21 1981-06-26 Fabre Sa Pierre Preparations immunostimulantes a base d'arn ribosomaux et procede de preparation des arn
US4830969A (en) * 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
DE3211309A1 (de) * 1982-03-26 1983-09-29 Metin Dipl.-Ing. 6100 Darmstadt Colpan Chromatographisches verfahren zur isolierung von makromolekuelen
US4482482A (en) * 1983-01-17 1984-11-13 Pharmacia, Inc. Cesium trifluoroacetate solutions for the isolation of nucleic acids
US4514497B1 (en) * 1983-12-30 1998-02-24 Novagene Inc Modified live pseudorabies viruses
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
EP0245945B1 (de) * 1986-04-11 1993-11-10 Applied Biosystems, Inc. Automatisches Gerät zum Extrahieren von Nukleinsäuren
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US4833239A (en) * 1987-01-29 1989-05-23 Genetics Institute, Inc. Method for the isolation and purification of DNA molecules

Also Published As

Publication number Publication date
DE69030449T2 (de) 1997-10-16
EP0508985A4 (en) 1992-12-02
EP0747388A1 (de) 1996-12-11
EP0747387A3 (de) 1996-12-18
EP0747388B1 (de) 1999-09-22
EP0508985B1 (de) 1997-04-09
EP0747387A2 (de) 1996-12-11
US4997932A (en) 1991-03-05
DE69033304D1 (de) 1999-10-28
WO1991007422A1 (en) 1991-05-30
DE69033304T2 (de) 2000-02-03
DE69030449D1 (de) 1997-05-15
EP0508985A1 (de) 1992-10-21
ES2100887T3 (es) 1997-07-01

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Legal Events

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