ATE197479T1 - Verfahren zur vervielfältigung von nukleinsäuresequenzen - Google Patents

Verfahren zur vervielfältigung von nukleinsäuresequenzen

Info

Publication number
ATE197479T1
ATE197479T1 AT93306169T AT93306169T ATE197479T1 AT E197479 T1 ATE197479 T1 AT E197479T1 AT 93306169 T AT93306169 T AT 93306169T AT 93306169 T AT93306169 T AT 93306169T AT E197479 T1 ATE197479 T1 AT E197479T1
Authority
AT
Austria
Prior art keywords
duplication
nucleic acid
acid sequences
oligonucleotides
kits
Prior art date
Application number
AT93306169T
Other languages
English (en)
Inventor
Sherrol Mcdonough
Daniel Kacian
Nanibhushan Dattagupta
Diane Mcallister
Philip Hammond
Thomas Ryder
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ATE197479T1 publication Critical patent/ATE197479T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/702Specific hybridization probes for retroviruses
    • C12Q1/703Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT93306169T 1992-08-04 1993-08-04 Verfahren zur vervielfältigung von nukleinsäuresequenzen ATE197479T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US92540592A 1992-08-04 1992-08-04

Publications (1)

Publication Number Publication Date
ATE197479T1 true ATE197479T1 (de) 2000-11-11

Family

ID=25451694

Family Applications (2)

Application Number Title Priority Date Filing Date
AT93306169T ATE197479T1 (de) 1992-08-04 1993-08-04 Verfahren zur vervielfältigung von nukleinsäuresequenzen
AT99121906T ATE346954T1 (de) 1992-08-04 1993-08-04 Nachweis von mycobacterium tuberculosis durch vervielfältigung von nukleinsäuresequenzen

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT99121906T ATE346954T1 (de) 1992-08-04 1993-08-04 Nachweis von mycobacterium tuberculosis durch vervielfältigung von nukleinsäuresequenzen

Country Status (11)

Country Link
EP (2) EP0587298B1 (de)
JP (3) JP4372837B2 (de)
KR (1) KR100316498B1 (de)
AT (2) ATE197479T1 (de)
AU (2) AU670116B2 (de)
CA (1) CA2141430C (de)
DE (2) DE69329636T2 (de)
ES (1) ES2277679T3 (de)
NO (1) NO950381L (de)
TW (1) TW278099B (de)
WO (1) WO1994003472A1 (de)

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FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
JP3124036B2 (ja) * 1995-01-19 2001-01-15 ジェン−プローブ・インコーポレーテッド ライム病関連ボレリアに対する核酸増幅オリゴヌクレオチドおよびプローブ
US5622827A (en) * 1995-02-07 1997-04-22 Gen-Probe Incorporated Amplification primers and nucleic acid probes targeted to coccidioides immitis nucleic acid
ATE196322T1 (de) * 1995-05-05 2000-09-15 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
US5925518A (en) * 1995-05-19 1999-07-20 Akzo Nobel N.V. Nucleic acid primers for amplification of a mycobacteria RNA template
JP3996639B2 (ja) * 1995-06-07 2007-10-24 ジェン−プローブ・インコーポレーテッド 生成物の増幅後レベルから核酸標的配列の増幅前レベルを決定するための方法とキット
CA2222305A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Nucleic acid probes and amplification oligonucleotides for neisseria species
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5652126A (en) * 1995-06-07 1997-07-29 Gen-Probe Incorporated Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5739311A (en) * 1995-06-07 1998-04-14 Gen-Probe Incorporated Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
US5747252A (en) 1995-06-07 1998-05-05 Gen-Probe Incorporated Nucleic acid probes and amplification oligonucleotides for Neisseria species
DE69734576T2 (de) * 1996-03-01 2006-08-03 E.I. Dupont De Nemours And Co., Wilmington Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments
WO1997044488A2 (en) 1996-05-22 1997-11-27 Gen-Probe Incorporated Compositions and methods for the detection of mycobacterium kansasii
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
EP0946749A1 (de) 1996-11-20 1999-10-06 The Regents Of The University Of Michigan Mikrofabrizierte vorrichtungen sowie verfahren zur isothermen amplifikation von nukleinsäuren
GB9626074D0 (en) * 1996-12-16 1997-02-05 Cytocell Ltd Nucleic acids amplification assay
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
DK0975807T3 (da) 1997-05-02 2007-01-29 Gen Probe Inc To-trins hybridisering og indfangning af et polynukleotid
EP1004676B1 (de) * 1997-08-14 2006-03-15 Takara Bio Inc. Methoden zur dns vervielfältigung und material dafür
JP2002509443A (ja) * 1997-10-31 2002-03-26 ジェン−プローブ・インコーポレーテッド 核酸検出法
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法
AU4739600A (en) * 1999-05-17 2000-12-05 Mcgill University Method for subtracting cdnas by suppressing the synthesis of specifically targeted mrnas
US6294338B1 (en) 1999-07-23 2001-09-25 Gen-Probe Incorporated Polynucleotide amplification method
US6902891B2 (en) 1999-12-17 2005-06-07 Bio Merieux Process for labeling a nucleic acid
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
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AU2001291513B2 (en) * 2000-10-03 2007-06-07 Id+Plus Ltd Nucleotide detection method
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ES2300375T3 (es) 2000-10-23 2008-06-16 Gen-Probe Incorporated Composiciones y metodos para la deteccion del virus de la inmunodeficiencia humana 2 (hiv-2).
JP2002125688A (ja) * 2000-10-30 2002-05-08 Tosoh Corp C型肝炎ウイルスの高感度検出のためのオリゴヌクレオチドおよび検出法
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AU2003253651C1 (en) 2002-06-14 2010-06-03 Gen-Probe Incorporated Compositions and methods for detecting hepatitis B virus
US7115374B2 (en) 2002-10-16 2006-10-03 Gen-Probe Incorporated Compositions and methods for detecting West Nile virus
EP1694871B1 (de) 2003-12-19 2010-08-25 Gen-Probe Incorporated Zusammensetzungen, verfahren und kits zum nachweis der nukleinsäuren von hiv-1 und hiv-2
EP2975139B1 (de) 2004-09-30 2019-11-06 Gen-Probe Incorporated Verfahren zum nachweis und zur quantifizierung von hiv-1
AU2005333163B2 (en) 2004-11-09 2011-06-16 Gen-Probe Incorporated Compositions and methods for detecting Group A streptococci
DK1853706T3 (da) 2005-02-18 2011-03-07 Gen Probe Inc Prøve-fremstillingsfremgangsmåde med et alkali-chock
AU2006244460B2 (en) 2005-05-06 2010-04-08 Gen-Probe Incorporated Compositions and assays to detect influenza virus A and B nucleic acids
EP1955241B1 (de) 2005-11-14 2011-03-30 Gen-Probe Incorporated Parametrisches kalibrationsverfahren
CA2809734A1 (en) * 2006-05-24 2007-12-06 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of mycobacterium tuberculosis complex organisms in a test sample
EP1921156A1 (de) * 2006-11-10 2008-05-14 bioMerieux B.V. Verbesserte multiplexe Nukleinsäureamplifikation mittels blockierter Primer
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
EP1978111B1 (de) * 2007-04-02 2013-03-27 Gen-Probe Incorporated Zusammensetzungen, Kits und zugehörige Verfahren zur Erkennung und/oder Überwachung von Pseudomonas aeruginosa
EP2281070B1 (de) 2008-04-21 2015-01-21 Gen-Probe Incorporated Verfahren zum nachweis von chikungunya-virus
CA2756659A1 (en) 2009-02-26 2010-09-02 Gen-Probe Incorporated Assay for detection of human parvovirus nucleic acid
EP3301195B1 (de) 2010-07-12 2022-11-02 Gen-Probe Incorporated Zusammensetzungen und assays zur detektion der 2009 h1n1-influenza-a-virus-nukleinsäure in schweinen
EP2611932A2 (de) 2010-08-30 2013-07-10 Gen-Probe Incorporated Zusammensetzungen, verfahren und reaktionsmischungen für den nachweis von xenotropic murine leukemia virus-related virus (xmrv)
WO2013012708A1 (en) 2011-07-15 2013-01-24 Gen-Probe Incorporated Compositions and method for detecting human parvovirus nucleic acid and for detecting hepatitis a virus nucleic acids in single-plex or multiplex assays
WO2014018885A2 (en) 2012-07-27 2014-01-30 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
ES2795677T3 (es) 2013-03-15 2020-11-24 Gen Probe Inc Método, aparato y producto de programa informático para calibración
EP3601617B3 (de) 2017-03-24 2024-03-20 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis oder zur quantifizierung des parainfluenzavirus
EP4219770A3 (de) 2017-03-24 2023-08-16 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von rsv b in proben
WO2019032809A1 (en) 2017-08-11 2019-02-14 Gen-Probe Incorporated COMPOSITIONS AND METHODS FOR DETECTING STAPHYLOCOCCUS AUREUS
JP2021506274A (ja) 2017-12-15 2021-02-22 ジェン−プローブ・インコーポレーテッド 毒素産生Clostridium difficileを検出するための組成物および方法
GB2597566B (en) 2018-06-13 2023-02-01 Gen Probe Inc Compositions and methods for detecting group B streptococcus nucleic acid
EP3830302B1 (de) 2018-08-01 2022-10-05 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von nukleinsäuren des epstein-barr-virus
AU2019319203B8 (en) 2018-08-08 2025-10-09 Gen-Probe Incorporated Compositions, methods and kits for detecting Mycoplasma genitalium
JP7579779B2 (ja) 2018-08-21 2024-11-08 ジェン-プローブ・インコーポレーテッド ヒトサイトメガロウイルスを増幅、検出、または定量化するための組成物および方法
EP3856934A2 (de) 2018-09-27 2021-08-04 Gen-Probe Incorporated Zusammensetzungen und verfahren zur detektion von bordetella-pertussis- und bordetella-parapertussis-nukleinsäure
CA3116895A1 (en) 2018-10-22 2020-04-30 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human polyomavirus bk virus
CA3152309A1 (en) 2019-08-23 2021-03-04 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum
US20220333162A1 (en) 2019-09-05 2022-10-20 Gen-Probe Incorporated Detection of chlamydia trachomatis nucleic acid variants
AU2020402029B2 (en) 2019-12-09 2022-12-15 Gen-Probe Incorporated Quantification of polynucleotide analytes from dried samples
EP4582563A3 (de) 2020-03-04 2025-10-08 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von sars-cov-2-nukleinsäure
WO2023010008A1 (en) 2021-07-27 2023-02-02 Gen-Probe Incorporated Compositions and methods for detecting gastrointestinal pathogens
KR102544380B1 (ko) 2022-01-29 2023-06-16 (주)동방지퍼 지퍼용 슬라이더와 손잡이의 결착방법 및 이로 인해 제조된 지퍼
EP4282980A1 (de) 2022-05-23 2023-11-29 Mobidiag Oy Methoden zum verstärken einer nukleinsäure
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers
WO2024233375A1 (en) 2023-05-05 2024-11-14 Gen-Probe Incorporated Method and system for improving specificity of analyte detection using real-time nucleic acid amplification

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Also Published As

Publication number Publication date
ATE346954T1 (de) 2006-12-15
JP4372837B2 (ja) 2009-11-25
JP3860809B2 (ja) 2006-12-20
NO950381D0 (no) 1995-02-02
JP2006238892A (ja) 2006-09-14
EP0978570A3 (de) 2000-04-12
EP0587298A3 (en) 1995-08-30
TW278099B (de) 1996-06-11
KR950702999A (ko) 1995-08-23
ES2277679T3 (es) 2007-07-16
JPH07509368A (ja) 1995-10-19
CA2141430C (en) 2009-05-12
AU700253B2 (en) 1998-12-24
AU6802796A (en) 1996-12-19
JP2004194662A (ja) 2004-07-15
EP0978570B1 (de) 2006-11-29
DE69329636D1 (de) 2000-12-14
WO1994003472A1 (en) 1994-02-17
DE69334092T2 (de) 2007-07-05
EP0587298A2 (de) 1994-03-16
EP0587298B1 (de) 2000-11-08
CA2141430A1 (en) 1994-02-17
AU670116B2 (en) 1996-07-04
KR100316498B1 (ko) 2002-06-20
EP0978570A2 (de) 2000-02-09
DE69334092D1 (de) 2007-01-11
NO950381L (no) 1995-04-03
DE69329636T2 (de) 2001-03-01
AU4792093A (en) 1994-03-03

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